CAFSCs morphology comparison of complete medium with and without adding chicken serum. A, cell morphological of CAFSCs without adding chicken serum culture in 24 h; B, cell morphological of CAFSCs with adding chicken serum (5%) culture in 24 h. Few differences have been found between A and B; C, cell morphological of CAFSCs without adding chicken serum culture in 72 h; D, cell morphological of CAFSCs with adding chicken serum (5%) culture in 72 h,cell number and morphology are better compared with C. Sparklets are hemocytes which come from matrix and will disappear after passage.