Advances in Animal and Veterinary Sciences
Detection of Mycoplasma gallisepticum from field samples of Poultry using conventional PCR
Davinder Singh*1, Nand Kishor Mahajan1, Aman Kumar2, Sushila Maan2, Pawan Kumar2
1Departement of Veterinary Public Health & Epidemiology; 2Department of Animal Biotechnology, College of Veterinary Sciences,
Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar–125004 (Haryana)
*Corresponding author: drdev18@gmail.com
ABSTRACT
The present study reports the use of polymerase chain reaction (PCR) for detection of Mycoplasma gallisepticum (MG) infection in poultry using tissues directly. A total of 51 samples (trachea and airsacs) were collected from clinically suspected birds originating from district Hisar, India. PCR was carried out using specie specific primers for MG and identified 18 samples (35.3%). It is concluded that tissues may be used for rapid screening and detection of MG in poultry.
Key Words: PCR; Mycoplasma gallisepticum; Poultry
