Adv. Anim. Vet. Sci. 5(2): 83-91
ESBL Detection and Comparison of Antibiotics Resistance Pattern of Klebsiella Pneumoniae Isolated from Healthy and Acute Respiratory Tract Infected Camels
Sandeep Kumar Sharma, Kruthika Patel, Sunil Maherchandani, Brij Nandan Shringi
Department of Veterinary Microbiology and Biotechnology, Post Graduate Institute of Veterinary Education and Research (PGIVER), Jaipur; 2College of Veterinary Medicine, University of Minnesota (USA); 3College of Veterinary and Animal Science, Bikaner Rajasthan University of Veterinary and Animal Sciences (RAJUVAS).
Abstract | Emerging antibiotic resistance is a global problem which interfere with effective disease management in animals. Although antibiotic susceptibility screenings are frequently conducted in human practice, its use in veterinary is very limited and very few literatures are available on antibiotic resistance pattern of Klebsiella pneumoniae isolated from respiratory tract infections of camels. In this investigation, total 65 PCR (16S-23S rDNA internal transcribed spacer region) confirmed K. pneumoniae isolates were included. Among those, 47 were from diseased and 18 were from healthy camels. All isolates were screened for 26 antibiotics of various groups and found 100 % isolates were multidrug resistant, any single isolate from diseased camels was at least resistant to 9 antibiotics and isolate from healthy camel was resistant to more than 6 antibiotics. Out of 26 antibiotics, 100% (65) isolates were susceptible to imepenem but resistant to bacitracin, clindamycin, rifampicin and sulfadiazine. 15 (31.9%) isolates from diseased camels showed Extended Spectrum Beta-Lactamases (ESBL) phenomena while none of the isolate from healthy camels exhibited this activity. Antibiotics susceptibility pattern was found significantly different for seven antibiotics that is cefepime, ciprofloxacin, oxacillin (p≤0.05), erythromycin, norfloxacin, tetracycline and trimethoprim (p≤0.01) whereas non-significant difference for susceptibility pattern was observed for remaining 19 antibiotics. It has established pointer to a correlation between natural and acquired antibiotic resistance and substantial information of occurrence of ESBL activity in K. pneumoniae from camels. Further suggested molecular characterization of these isolates in regards of antibiotic resistance and also the genes responsible for ESBL activity.
Keywords | Klebsiella pneumoniae, ESBL, Antibiotics, Resistance, Camel.