Advances in Animal and Veterinary Sciences
Molecular Identification of Candida Species Isolated from Ears of Dogs Infected with Otitis externa by Detecting Internal Transcript Spacer (ITS1 and ITS4) in Sulaimania, Iraq
Hanan Hasan Ali1*, Rana Mohamed Al-Obaidi2, Chiman Hamarahim Fattah1
1Department of Microbiology; 2Department of Basic Science, School of Veterinary Medicine, University of Sulaimania, Sulaimania, Iraq.
*Correspondence | Hanan Hasan Ali, University of Sulaimania, Sulaimania, Iraq; Email: dr.hananh23@gmail.com
Abstract
The goal of this study was to determine internal transcribed spacer/5.8S ribosomal DNA (rDNA) to detect Candida spp. in dogs with Otitis externa. PCR-based detection of internal transcribed spacer 2 (ITS2) regions of the rRNA genes was evaluated as a means of fungal identification by using Internal transcribed spacers 1 (ITS1) and Internal transcribed spacers 4 (ITS4). These were amplified by PCR to detect fungal DNA. Sixty swab ear samples from domestic dogs with and without O. externa were examined for clinical signs including head shaking, scratching the external ear flaps, bad odor, obstraction of the ear canal, anorexia, emaciation and auricular discharge (unilateral in 20 and bilateral in 10 dogs). The isolation of Candida species was performed from the external otitic canal in all animals. Yeast species isolated in 30 cases involving mainly Candida spp. (20 cases) and more often Aspergillus niger (5 cases), Penicellium spp. (3 cases) and Aspergillus fumigatus (2 cases). Twenty isolates of Candida used to test the selected primers and conditions of the PCR. Molecular fungal identification is successful in most isolates; with a band size 600-800 bp. This study concluded that size of PCR-amplified ITS2 region DNA is a rapid and reliable method to identify clinically significant yeasts.
Keywords | Candida spp, Candida famata, Candida kefyr, Otitis externa, Dogs, Colony PCR
Editor | Kuldeep Dhama, Indian Veterinary Research Institute, Uttar Pradesh, India.
Received | June 27, 2015; Revised | July 17, 2015; Accepted | July 19, 2015; Published | July 29, 2015
Citation | Ali HH, Al-Obaidi RM, Fattah CH (2015). Molecular identification of Candida species isolated from ears of dogs infected with Otitis externa by detecting internal transcript spacer (ITS1 and ITS4) in Sulaimania, Iraq. Adv. Anim. Vet. Sci. 3(9): 491-499.
ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331
Copyright © 2015 Ali et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
