Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
...efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic diversity. Whole genomic analysis of RVAs has revolutionized the study of RVA genomics, providing a plethora of conclusive and vital data on the overall genetic diversity, true origin and complex evolutionary patterns of human RVA str...
Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*
...re are three inactivated vaccines of H9N2 AIVs have been used in domestic poultry in China. But H9N2 AIVs occurred frequently and caused severe economic losses to the poultry industries. H9N2 AIVs can evade the pressure of vaccination by genetic evolution and antigen variation. Moreover, the ratio of L226 in the haemagglutin (HA) receptor binding site has increased, which indicates that the H9N2 virus has the increasing potential to infection and cause human e...
Ye Liu2, Yulong Cong2, Yiming Shao1*
...ving immune responses of vaccines. Herein, we review recent developments of HIV Tat as immunoregulator using for optimizing the efficacy of vaccines. By highlighting preclinical/clinical applications of Tat and its working mechanisms, this review exhibits the superior potential of Tat as immunoregulator on optimizing immune responses mediated by vaccines. These examples of selected studies...
James Ayukepi Ayukekbong1,2
E-mail | james.ayukekbong@microbio.gu.se
...re currently no licensed vaccines or specific therapeutics. The virus and its vector are widely distributed throughout the tropics and subtropics regions of the world. Dengue and malaria are endemic in Nigeria. Two independent studies among febrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever ...
Shen Yang1, Guang-Zhi Tong1, 2*
...ment of novel protective vaccines. In this review, we discuss the innate immune responses against HP-PRRSV infection and suggest new areas of investigation into this important interface between pathogen and host....
Huaichang Sun
...otection of conventional vaccines. RNA interference (RNAi) has becoming a feasible strategy against various virus infections. Recently, a significant advance in RNAi technology is the use of artificial microRNAs (amiRNAs) to fight virus infections. However, different strategies are needed to prevent virus variation or mutation escape. This review is intended to present the current situation of antiviral roles of amiRNAs, against variable RNA viruses in particu...
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg
...ovements and inactivated vaccines were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is st...
Kang-Seuk Choi
...ative to inactivated IBD vaccines. In addition, VLP technology has many applications. This paper reviews the potential of VLPs as an alternative to IBD vaccines, along with their specific applications in the field....
Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*
...g diagnostic methods and vaccines for classical swine fever....
Moustafa Kardjadj1, Pam Dachung Luka2
...equate supply of quality vaccines and the problem of delivery to maintain cold chain, there is need for a very strong regional coordination for effective and sustainable control of PPR in Africa. The African continent is currently divided into 57 countries (or independent territories), many of which rely heavily on livestock for fuel, motor power and sustenance with many also endemic to the disease. PPRV continues to decimate the small ruminant populations acr...
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
...s for the development of vaccines and diagnostics for differentiating infected and vaccinated animals. With the advent of recombinant DNA technology, recombinant protein based vaccines and/or diagnostics are being tested in various heterologous systems across the globe for development of vaccines and/or diagnostic antigens. The recombinant viral proteins, virus like particle based
Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5
...he efficacy of live IBDV vaccines on broilers. A total of 120 broilers were randomly divided into six groups (20 birds in each) were coded A, B, C, D, E and F; vaccinated with various combinations of IBD through different routes. Results showed that the birds of different groups received different maternal antibodies did not induce any antibodies as recorded in all groups received vaccines at days 1, 9 or 12 exhibited simila...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
...ality inactivated rabies vaccines. Rapid Fluorescence Focus Inhibition Technique (RFFIT) revealed mRVNA titer (>0.5 IU/mL) in 29 puppies while 48 puppies showed mRVNA titer less than minimum protective level (0.2-0.4 IU/mL). RFFIT did not reveal mRVNA in 23 puppies that were declared lacking maternal rabies immunity. One basis of findings of this study it was concluded that puppies population lacking maternal rabies immunity is one of the responsible factor...
John G. Bruno
...lso serve as rickettsial vaccines, if fully developed. The top five highest affinity R. rickettsii and top five R. typhi anti-Fab aptamers as assessed by enzyme-linked aptamer sorbent assay (ELASA) screening were shown to specifically bind their cognate antibodies when captured by their Fc tails on the surface of Protein A-conjugated magnetic beads. While no clear similarities in the partial or complete primary DNA sequences or secondary stem-loop structures o...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...cost-effective influenza vaccines is urgently needed to safeguard poultry and to contain zoonosis. The primary goal of this study was to develop H9 plasmid-based DNA vaccine against the currently circulating AIV subtype H9N2 targeting HA gene of the virus A/Turkey/Egypt/1341V/2013(H9N2). The full length sequence of HA gene was codon optimized to the chicken biased codons and subcloned into the pCAGGS plasmid vector under the control of the chicken β-actin...
Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1
...Inactivated water-in-oil vaccines are widely used to control viral diseases in poultry, however, the interference of physico-chemical properties of the emulsions are overlooked when compared to antigenic properties. This study was designed to assess the effect of two different commercial inactivated water-in-oil multivalent vaccines; vaccine A contains IBV (Massachusetts M41 and D274 strains), NDV (Clone 30), aMPV (BUT 8544)...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
... reduces the efficacy of vaccines prepared to a particular clone of HBV isolates.
... 
Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***
ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...as pre-treatment with ND vaccines as it significantly reduced morbidity and mortality as compared to the use of vaccines alone. However, further work is recommended in future on GZ-08™ for its use as post-treatment of ND as well as on other antiviral compounds of natural origin to develop a novel antiviral drug against NDV in poultry....
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...animals, live attenuated vaccines have been suggested to provide protective immunity in the African wild dog; however, their use has been hampered by the fear of vaccine-induced disease. Kolmården Wildlife Park in Sweden has used a live attenuated vaccine without any apparent cases of vaccine-induced disease, but no studies to evaluate immune response after vaccination have been performed. The objective of this study was to gain more knowledge about...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
...immunogenicity of toxoid vaccines and bacterin toxoid vaccine were studied and compared initially in rabbits and then in goats and sheep. Experimental trials were conducted on 54 healthy animals of each species and were divided into three equal groups (n=18). The antibody titers were evaluated with indirect haemagglutination test. The results indicated significantly higher (P< 0.05) immune titer in animal group vaccinated with toxoid vaccine prepared from p...
Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2
...ous biological controls, vaccines and local medicinal plants are being explored to control the parasitic worms. The aim of this study was to evaluate the anthelmintic potential of two local plants, Albizia lebbeck L. and Camellia sinensis L against H. contortus using Adult Motility Assay (AMA). Crude Ethanolic Extracts (CEE)of dried leaves of A. lebbeck L. and whole plant of C. sinensis L. were prepared by cold ma...
Omer Baris Ince*
... performed with inactive vaccines of suitable serotype and measures are taken in order to reduce the prevalence of the disease. In this study, blood samples were collected from clinically healthy sheep and bovine in the surrounding region of the southeast of Konya province that were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aim...
Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1*
... Newcastle disease virus vaccines can provide various levels of protection against challenge with different NDV genotypes, raising the importance of the relationship between vaccines and field strains. The aim of the current work is to evaluate the effect of moisture percentage and pH on the persistence of velogenic NDV genotype VII in the poultry manure which considers a major threat to the Egyptian poultry industry since 2...
Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1*
... homologous/heterologous vaccines especially the live ones. The aim of the current work is to estimate the persistence time for velogenic Newcastle disease virus (NDV) of genotype VII in poultry manure in compare to LaSota vaccine strain as a model of genotype II. Velogenic NDV of genotype VII are a major threat to the Egyptian poultry industry since 2011 onwards with continuous evolution and diversity. In the present study, manure from specific pathogen free ...
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
...tin 28 (SS-28)DNA vaccines in promoting lactation of rats. Fifteen female SD rats were randomly divided into three groups and were orally vaccinated, respectively SS-14 (Group T1) and SS-28 (Group T2) DNA vaccines fused tPA signal peptide and CpG adjuvant and delivered by attenuated Salmonella choleraesuis at weeks 0, 3 and 6 of the study, and rats in control group (Group C) w...
Rana Waqas Arshad1, Asim Aslam1, Muhammad Saeed Imran1, Kamran Ashraf2 and Raheela Akhtar1,*
...e commercially available vaccines of infectious bursal disease (IBD). Three experimental groups, each having 100 broiler birds were placed in the same environmentally controlled house. Samples of blood and bursa of Fabricius were collected from slaughtered birds after every 7 days till the end of experiment (35 days). The size of bursas and the bursa/body weight ratios were significantly greater in live vector vaccine group than other vaccinal groups. ELISA re...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...iv>Currently a number of vaccines are under development against hepatitis C, though an effective hepatitis C vaccine is yet to come. The present study is focused on the development of recombinant vaccine against HCV by utilizing HCV3a envelope glycoprotein E2. HCV3a E2 gene was amplified and cloned first in cloning vector pTG19 and subcloned in expression vector pET21a. E2 protein, expressed in insoluble form, was purified by repeated sonications, follo...
Yan-Guo Han, Yu-Qing Han, Jia-Yuan Wu, Jia Guo, Ri-Su Na, Yan Zeng, Guang-Xin E, Yong-Ju Zhao and Yong-Fu Huang*
...oup (Group C), and these vaccines were given orally into the ewes at weeks 0, 3 and 6 of the research. Blood samples of ewes or offspring lambs were collected at week 6 or 9 of lactation, respectively. Serum SS, prolactin (PRL), growth hormone (GH) and insulin-like growth factor 1 (IGF-1) concentrations were detected by radioimmunoassay. The offspring lambs in T group showed significantly lower serum SS and higher PRL concentrations than those in the control g...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
...njected KISS1 DNA vaccines on immunocastration and in inhibiting mutton odour of male goats. In this study, 20 male goats aged eight weeks were randomly divided into the oral and plasmid-injected KISS1. DNA vaccine groups (groups T1 and T2) and the empty vector groups (groups C1 and C2) for immunisation. Blood samples were collected at 0, 3, 6, 10 and 14 weeks after primary immunisation for the detection of kisspeptin antibodies and testosterone ...
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...>Despite the presence of vaccines, Newcastle disease (ND) and Infectious Bronchitis (IB) outbreaks are not uncommon in endemic countries. Therefore, the current study was conducted to investigate the efficacy of commercially available vaccines against each virus alone as well as co-infections with NDV and IBV in commercial poultry. For assessment of vaccine efficacy, commercial chickens were vaccinated with La Sota (against ...
Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5*
...ly contrasted by current vaccines. ...
Rabia Riaz1, Khushi Muhammad1*, Masood Rabbani1, Muhammad Amjad Iqbal2, Aamir Riaz Khan2, Sadia Sarfaraz1, Mudassar Naseer3 and Khalid Majeed1
... Disease Virus) and O-MG vaccines, respectively. Serum samples were collected on 21, 28 and 35 days post vaccination for anti-MG-ELISA antibody titer. Cumulative mean anti-MG-ELISA antibody titer of birds to G-MG, O-MG, G-MG+NDV, O-MG+NDV and control was 80.30, 137.49, 91.59, 169.76 and 0.15 units respectively on 35 days post-vaccination. It is concluded that NDV has insignificant immunomodulatory effect on antibody response of birds to the MG vaccine.<...
Walaa ,S. Shabanaa;Bakr, A. Abdallaa; Eman, M. ELShalamia; I.M. Redab; M. A. Shalabyb
... compares it with single vaccines either FMD or RFV alone.The
mean of antibody titers continued with the protective level till the 32 to 36th week post vaccination,
In single vaccination either FMD or RFV alone while till to 40th WPV in combined FMD/RVF
vaccine.In the final we can conclude that the use of Montanide ISA 50 as an oil adjuvant in prepared
vaccines improve the immu...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...xpressing H5 (rFP-AI-H5) vaccines were measured. Vaccine efficacy was evaluated by percentage of protection from mortality, morbidity and reduction in virus shedding from respiratory and/orintestinal tracts. Despite the H5 antibody responses in vaccinated chickens with program I (rFP-AI-H5" vaccine then Re-5) being significantly lower prior to challenge, it provided good protection (73.3%) against the lethal A/chicken/Egypt/SHAH-1403/2011 (H5N1) AIV chall...
Walaa Abd El-Fatah S. Metwally1, Ismail M. Reda2, Ahmed A. El-Sanousi2, Mohamed Abd El-Khalek Ali2
...train H5N1. The prepared vaccines with Montanide ISA70VG varied in the HA units per dose and also differed in the ratios between two reassortant strains (Chicken and duck strains) of AI. The results of testing humoral immunity revealed that the prepared vaccines with Montanide ISA70VG containing 350 HA unit/dose were high in antibody titer than vaccines containing 256 and 100 HA unit/dose....
Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***
...inactivated oil adjuvant vaccines against infectious coryza and infectious bronchitis diseases were prepared, one adjuvanted with mineral oil and the other with Montanide ISA-70. Evaluation of both vaccines were done by challenge test, haemagglutination inhibition test, ELISA test for Avibacterium Paragallinarum, and ELISA test, indirect haemagglutination inhibition test and ciliostasis score for infectious Bronchitis virus ...
1Mona A. El-Manzalawy, 2Abeer A. Boseila , 3Hanan M. El Zahed
...y and sterility of these vaccines and compare between them with the local RVF vaccine with Alum gel alone in sheep. This peppermint formulation including Alum gel adjuvant was the best of choice as it induced higher immunological enhancement. In addition, synergistic action was discovered in RVF inactivated PMO vaccine including Alum gel as the immune response detected by serum neutralization test (SNT). This vaccinated group of sheep was the highest while the...
Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1
...etions. Extensive use of vaccines has not eliminated EHV infections, and the worldwide annual financial burden from these equine pathogens is immense. Clinical signs may occur despite the presence of a virus-specific immune response in the horse. The current study focus on outbreak recorded in El Zahraa Stud for Arabian horses during 2014 and recorded officially for the first time by OIE in Egypt by the notification presented by Chairman, General Organization ...
Amir M. Walaa1 ; Ausama Yousif1; Walid H. Kilany2; Magdy El-Sayed1; Ahmed A. El-Sanousi1
...ined inactivated NDV+IBV vaccines and investigate its ability to protect experimental broiler chickens against challenge with wild ND and IB viruses. Two hundred and eighty commercial one-day-old broiler saso chicks were divided into 8 groups (35 birds each). Groups 2, 3, 4&5 were vaccinated at one-day-old with live hitchner + IB Primer. Then all groups at 7 days old were boosted with inactivated combined NDV+IBV vaccines
Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar
...icken anemia virus (CAV) vaccines. One was vaccinated with 26 P4 strain vaccine subcutaneously and the other was vaccinated with Cux strain vaccine through drinking water (DW). Then the inactivated avian influenza (AI) H5N1 vaccine was inoculated intra-muscularly either at the 1st, 2nd or 3rd WPV with CAV vaccine forming three subgroups per each group. It is clearly observed that a relatively high levels of CAV ELISA antibody titers in all the vaccinated chick...
Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and M. A. Shalaby2
...y of four batches of PPR vaccines was tested as per the Office International des Epizooties (OIE) guidelines and Couacy-Hymann et al., 2002 using RT-PCR technique that based on the amplification of fragments of N-protein. All batches are also subjected to sterility tests for detection of bovine viral diarrhea virus as possible extraneous virus contaminant using RT-PCR and detection of mycoplasma as possible bacterial contaminant using PCR technique. All of the...
Lamya A. F. Ateya1 , Said. A .Ahmed2, Khamees K.S. Ashraf3, Heba A. Abdel-Hady4
...y local and imported FMD vaccines in cattle sera in Kalubeya Governorate. Methods: 220 serum samples were tested for FMD NSP by 3ABC Trapping ELISA.20 serum samples were used as control (no vaccination),100 serum samples collected from animals received local vaccine and 100 serum samples from animals received imported vaccine. Results: 34/220(15.5%) were +ve and 186/220(84.5%)were –ve for FMD NSP test preinoculation with vaccines...
Ola Youssef 1; EL-Deeb A.H.2 Nassif S.A.1 and Ahmed A. El-Sanousi 2.
...combinant H5
vaccines had been established yet so, these vaccines are evaluated by applying the conventional QC
tests such as identity, safety, purity, potency and efficacy that were not satisfactory in vaccine
evaluation.
Objective: The present study was conducted to standardize a protocol for quality control and
evaluation of the recombinant HVT-H5 vaccine.
...
Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and Mohammed A. Shalaby2
...ods: Four batches of PPR vaccines that produced in Egypt by Veterinary Serum and Vaccines
Research Institute (VSVRI) were collected and tested for identification of PPRV by conventional RTPCR
using two primer sets targeting F-gene and N-gene and real time RT-PCR.
Results: By F-gene based RT-PCR, PPRV could be detected in only 2 samples out of 4samples (50%).
By N-gene based RT-...
Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden
...vaccinated birds with AI vaccines under strictly controlled condition with
virulent HPAI virus may also be used to predict flock response to AI infection. The reduction in
challenge virus replication can be quantified using classical virus isolation (VI) and titration methods
in (ECE) or by molecular assay for detection AI virus specific nucleic acids such as (rRT-PCR).
Methods: Titration of the HPAI virus for in...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...luenza virus
vaccines is urgent to reach protective antibody titers and reduce virus shedding.
Methods: Groups of one-day old commercial broiler chicks were divided in to 8 groups 1, 2 and 3 were
vaccinated with a prepared vaccine contain 500HAU of H5N1 reassortant antigen; while group 4, 5 and 6
were vaccinated with an imported reassortant vaccine with 500HAU antigen content of H5N1 at 1, 5 and 10
da...
Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2
...uation methods of the AI vaccines are very important
for controlling the license of the bad vaccines. Comparative studies between the invitro and invivo
methods for 8 types of AI vaccines (5 batches for each) to evaluate their efficacies were done.
Methods: The invivo method, was done through the vaccination-challenge pathway of SPF chickens
...
Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2
...ent
and safe vaccines.
Aim: The present study investigated the effect of simultaneous administration of inactivated AI
vaccine and live attenuated TRT vaccine on the immune response of turkey chickens.
Methods: Four groups of bronzy turkey chickens were used in this study; group-1 received live
attenuated TRT vaccine, group-2 received inactivated AI vaccine, group-3 received TRT and AI
Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2
...assical IBDV
vaccines and demonstrated IBD characteristic lesions.
Objective: In present study the aim was to molecular characterize IBDV field strains detected in flocks
located in 4 governorates. Also, a phylogenetic analysis based on the sequence of the hyper variable
region of VP2 gene was carried out.
Methods: Forty field bursa samples were collected from different localities. IBDV was detected u...
Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2
... develop the recommended vaccines. Objective: Our study aimed for Molecular Diagnosis of Avian influenza virus (AIV) from Different Avian Species that would be useful to detect the current strains present in Egypt to use it in vaccination program. Methods:In this study, we collected 122 sample representing 12 different area in Egypt and seven different species of poultry produced and traded in Egypt. These samples were isolated on SPF eggs, detected for AIV in...
Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2
... current live attenuated vaccines against CaPVs. The virus isolation,
identification, and vaccine or other diagnostic preparations mainly depend on the proper culture used.
Objective: Our aim was to study the growth kinetic of recent LSDV isolate to determine the culture
properties of recent LSDV isolated from skin nodule that could be useful in the attempts to develop
more effective LSDV vaccine and diagnostic p...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
...VII inactivated and live vaccines initiated a high level of antibody
titers that can limit the transmission and virus shedding to the contact birds. These results
may help in controlling the NDV of GVII virulent strain that is commonly circulating in the
field.
...
Theophilus Miebi Gbigbi* and Rodney Akpoviri Isiorhovoja
... chicks, feed, drugs and vaccines, labour, shelter and equipment significantly influenced exotic chicken production. The resource use efficiency result infers that DOCs and drugs and vaccine were under-utilized while feeds, labour and housing and equipment were over-utilized by the farmers. The elasticity of production was 1.08. This is increasing returns to scale. This paper concludes that poultry farmers were inefficient in the application of productive reso...
Mustafa Ozan Atasoy
...fer and efficient vector vaccines, which convey immunogenic genes of interest obtained from vitally important diseases. Herpesvirus of turkeys (HVT) has long been considered a versatile tool owing to its non-pathogenic characteristic, relatively higher transgenic capacity, and enabling the DIVA strategy. Numerous bivalent and multivalent HVT constructs have been generated by various methods and some of these constructs have been made commercially available so ...
Sameh Abdel-Moez Ahmed Amer1*, Mohamed Abdel-Aziz Kutkat1, Mohamed Mahmoud Abdel-Baki1, Asmaa Mahmoud Maatouq1, Omnia Mohamed Kutkat2, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1
... Egypt. The conventional vaccines can no longer provide an adequate protection for birds against ND outbreaks, where expected to be due to genotype mis-matched vaccines to the rapidly evoluted NDV field strains. Herein, the present study investigated the prevalence and the phylography of currently epidemic genotypes of NDV. Out of 550 samples were collected during an active surveillance in 2020 and 2021 from different poultr...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...d evaluation of the used vaccines are required.
Keywords | IBDV, Identification, Phylogenic tree, Native chickens, RT- PCR
...
Safaa M. Barghash1*, Amani A. Hafez2
... pasteurellosis with two vaccines; one is commercial, and the other is a formalin-killed vaccine (prepared from a local strain), and subsequently challenged virulent P. multocida. The differences in the regulation of IFN-γ, TNF-α, IL12, IL6, and IL10 cytokine levels expression, were assessed as biomarkers of the clinical phase of infections. Results revealed that T. evansi infection has a significant influence on the performance of rats, and antibo...
