Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
...olates. The O/PAK Lahore vaccine was at two mutations from central node, while HE647819 and HE647820 were clustered into a separate single taxon at eightieth (80) mutations from central node. Taken together, these findings highlight the continuous circulation of serotype O of FMD in the region.
...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
...olates. The O/PAK Lahore vaccine was at two mutations from central node, while HE647819 and HE647820 were clustered into a separate single taxon at eightieth (80) mutations from central node. Taken together, these findings highlight the continuous circulation of serotype O of FMD in the region.
...
Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
... genes are important for vaccine development or judging the efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic diversity. Whole genomic analysis of RVAs has revolutionized the study of RVA genomics, providing a plethora of conclusive and vital data ...
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
...olates. The O/PAK Lahore vaccine was at two mutations from central node, while HE647819 and HE647820 were clustered into a separate single taxon at eightieth (80) mutations from central node. Taken together, these findings highlight the continuous circulation of serotype O of FMD in the region....
Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*
...re are three inactivated vaccines of H9N2 AIVs have been used in domestic poultry in China. But H9N2 AIVs occurred frequently and caused severe economic losses to the poultry industries. H9N2 AIVs can evade the pressure of vaccination by genetic evolution and antigen variation. Moreover, the ratio of L226 in the haemagglutin (HA) receptor binding site has increased, which indicates that the H9N2 virus has the increasing potential to infection and cause human e...
Ye Liu2, Yulong Cong2, Yiming Shao1*
...ortant immunogen for HIV vaccine candidates for a long time. However, HIV Tat also plays immunoregulation roles in improving immune responses of vaccines. Herein, we review recent developments of HIV Tat as immunoregulator using for optimizing the efficacy of vaccines. By highlighting preclinical/clinical applications of Tat and its working mechanisms, this review exhibits the superior pot...
Xingdong Yang and Lijuan Yuan
...immune responses and for vaccine evaluations. These are the basis for the successful establishment of the infection and disease models for human rotavirus, human norovirus and human enterovirus 71 using neonatal gnotobiotic pigs. The differences between germ-free and normal animals in the maturation status of immune systems caused by the lack of gut microbiota can be minimized by establishing human-gut-microbiota transplanted gnotobiotic pig models. Given the ...
James Ayukepi Ayukekbong1,2
E-mail | james.ayukekbong@microbio.gu.se
...re currently no licensed vaccines or specific therapeutics. The virus and its vector are widely distributed throughout the tropics and subtropics regions of the world. Dengue and malaria are endemic in Nigeria. Two independent studies among febrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever ...
Shen Yang1, Guang-Zhi Tong1, 2*
...ment of novel protective vaccines. In this review, we discuss the innate immune responses against HP-PRRSV infection and suggest new areas of investigation into this important interface between pathogen and host....
Huaichang Sun
...otection of conventional vaccines. RNA interference (RNAi) has becoming a feasible strategy against various virus infections. Recently, a significant advance in RNAi technology is the use of artificial microRNAs (amiRNAs) to fight virus infections. However, different strategies are needed to prevent virus variation or mutation escape. This review is intended to present the current situation of antiviral roles of amiRNAs, against variable RNA viruses in particu...
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg
...ovements and inactivated vaccines were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is st...
Muhammad Arif Zafar1, Murtaz-ul-Hasan1*, Aayesha Riaz1, Asim Shamim2, Muhammad Farooq Iqbal1 and Arfan Yousaf
E-mail | murtazhassan@yahoo.com
...utic value of autogenous vaccine along with immune-modulators and to compare its efficacy with autogenous vaccine alone and partial excision of the lesions. To this end, nine animals were selected clinical and sporadic papillomas different body parts. These animals were divided into three groups; Group A animals were operated for partial excision of the lesions, Group B were inoculated with autogenous
Kang-Seuk Choi
... promising approaches to vaccine development and are an alternative to inactivated IBD vaccines. In addition, VLP technology has many applications. This paper reviews the potential of VLPs as an alternative to IBD vaccines, along with their specific applications in the field....
Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*
...g diagnostic methods and vaccines for classical swine fever....
Behzad Hussain1, 2, Abdullah Iqbal2, Muhammad Abubakar2*
...o commercially available vaccine for this disease. It is recommended that the disease can be controlled by adopting preventive measures such as avoiding of tick bites and contact with blood of the suspected animal. ...
Moustafa Kardjadj
...quent replacement of the vaccine strains. The present review summarizes the knowledge generated in epidemiology, diagnosis and surveillance of the disease in the Maghreb countries....
Vinayagamurthy Balamurugan*, Gurrappa Naidu Govindaraj and Habibur Rahman
... to the effectiveness of vaccine, timely vaccination, circulation of a single lineage virus and most importantly effective planning and implementation of the vaccination programme. Sharing the experiences on the PPR control strategies adopted by some of the states in India may motivate other Indian states or other countries of similar socio-economic and small ruminant rearing pattern to vaccinate and control PPR....
Moustafa Kardjadj1, Pam Dachung Luka2
...equate supply of quality vaccines and the problem of delivery to maintain cold chain, there is need for a very strong regional coordination for effective and sustainable control of PPR in Africa. The African continent is currently divided into 57 countries (or independent territories), many of which rely heavily on livestock for fuel, motor power and sustenance with many also endemic to the disease. PPRV continues to decimate the small ruminant populations acr...
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
...s for the development of vaccines and diagnostics for differentiating infected and vaccinated animals. With the advent of recombinant DNA technology, recombinant protein based vaccines and/or diagnostics are being tested in various heterologous systems across the globe for development of vaccines and/or diagnostic antigens. The recombinant viral proteins, virus like particle based
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...h genotype including the vaccine strains (genotype II and III). Interestingly, some of these mutations were found exclusive to the study isolate. Not only do these prime findings improve our understanding about currently circulating strains of NDVs but they also help us to envisage potential efforts to avoid future outbreaks in wild birds, as well as in commercial and backyard poultry.
...
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
... potentially applied for vaccine production, better vaccination protocols and infection control in the future....
Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5
...he efficacy of live IBDV vaccines on broilers. A total of 120 broilers were randomly divided into six groups (20 birds in each) were coded A, B, C, D, E and F; vaccinated with various combinations of IBD through different routes. Results showed that the birds of different groups received different maternal antibodies did not induce any antibodies as recorded in all groups received vaccines at days 1, 9 or 12 exhibited simila...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
...ality inactivated rabies vaccines. Rapid Fluorescence Focus Inhibition Technique (RFFIT) revealed mRVNA titer (>0.5 IU/mL) in 29 puppies while 48 puppies showed mRVNA titer less than minimum protective level (0.2-0.4 IU/mL). RFFIT did not reveal mRVNA in 23 puppies that were declared lacking maternal rabies immunity. One basis of findings of this study it was concluded that puppies population lacking maternal rabies immunity is one of the responsible factor...
John G. Bruno
...lso serve as rickettsial vaccines, if fully developed. The top five highest affinity R. rickettsii and top five R. typhi anti-Fab aptamers as assessed by enzyme-linked aptamer sorbent assay (ELASA) screening were shown to specifically bind their cognate antibodies when captured by their Fc tails on the surface of Protein A-conjugated magnetic beads. While no clear similarities in the partial or complete primary DNA sequences or secondary stem-loop structures o...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...cost-effective influenza vaccines is urgently needed to safeguard poultry and to contain zoonosis. The primary goal of this study was to develop H9 plasmid-based DNA vaccine against the currently circulating AIV subtype H9N2 targeting HA gene of the virus A/Turkey/Egypt/1341V/2013(H9N2). The full length sequence of HA gene was codon optimized to the chicken biased codons and subcloned into the pCAGGS plasmid vector under the...
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
... (H5 and ND) inactivated vaccine via subcutaneous route. Birds (n=20) were then either challenged with 106 EID50 using Egyptian avian influenza H5N1 and Newcastle disease genotype VII. Comparative blood profiling in vaccinated and challenged, non-vaccinated and non-challenged and non-vaccinated and challenged indicated that MDA for H5N1 gradually decreased from 5.1 to 0.4 log2 and disappeared with in a month. While MDA for NDV remained 2.1 log2 until 28th day ...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...t 2.4 million people get vaccine for allergy from Allergy Centre, National Institute of Health (NIH), Islamabad. Human Leukocyte Antigens (HLA) genes are located on the short arm of the chromosome number 6 and position 21 and the allele frequency is associated with allergy. The study was conducted to investigate the relationship between the aero-allergens and the frequency of HLA-DRB1*11 and HLA-DRB1*12 allele in aeroallergen sensitized in...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...Egy-H5 plasmid DNA-based vaccine against a currently circulating avian influenza virus H5N1 strain in Egypt targeting the HA gene. The HA gene of AIV Egyptian strain H5N1 was codon optimized for chicken biased codon and sub-cloned into pCAGGS mammalian expression vector under the control of chicken β-actin promoter. The construct was transfected into 293T HEK (Human Embryonic Kidney) cell line for in vitro expression of the recombinant plasmid DNA. The co...
Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1
...Inactivated water-in-oil vaccines are widely used to control viral diseases in poultry, however, the interference of physico-chemical properties of the emulsions are overlooked when compared to antigenic properties. This study was designed to assess the effect of two different commercial inactivated water-in-oil multivalent vaccines; vaccine A contains IBV (Massachusetts M41 and D274 strai...
Kashif Prince1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Atif Prince3, Naimat Ullah Khan1 and Mumtaz Ali Khan1
...alves against FMD and HS vaccine. Thirty-five pregnant cattle were selected and seven groups were made consisting five cattle. Group A was controls: Animals in Group B and C were at 45 days prepartum with and without VES: animals in D and E were at day 60 prepartum with and without VES: animals in F and G were at 75 days prepartum stage with and without VES: respectively. At day 0 of experimental trial VES was given to animals in group C, E and G. All groups w...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...t fertile eggs for virus vaccine production.
...
Saba Rafique1,2,*, Khalid Naeem1, Naila Siddique1, Muhammad Athar Abbas1, Aamer Ali Shah2, Akbar Ali1, Abdul Rahim1 and Farooq Rashid1
...ern from other known IBV vaccine strains, the isolate was named as KU145467_NARC/786_Pakistan_2013 (also named as Pak-786) was subjected to Spike gene sequencing covering the amino-terminus region of 01 to 974 base pairs. The S protein sequence was submitted to the GenBank with accession number KU145467. Phylogenetic grouping and maximum nucleotide sequence identity values were used to identify the isolate that looked to be derived from recombination. It showe...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
... reduces the efficacy of vaccines prepared to a particular clone of HBV isolates.
... 
Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***
ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...as pre-treatment with ND vaccines as it significantly reduced morbidity and mortality as compared to the use of vaccines alone. However, further work is recommended in future on GZ-08™ for its use as post-treatment of ND as well as on other antiviral compounds of natural origin to develop a novel antiviral drug against NDV in poultry....
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...animals, live attenuated vaccines have been suggested to provide protective immunity in the African wild dog; however, their use has been hampered by the fear of vaccine-induced disease. Kolmården Wildlife Park in Sweden has used a live attenuated vaccine without any apparent cases of vaccine-induced disease, but no studies to evaluate immune respo...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
... was to prepare a toxoid vaccine from the prevailing pathogenic strains of Type D Clostridiumperfringens strains and evaluate its immune responses in rabbits, goats and sheep. C. perfringens were isolated from enterotoxaemia suspected sheep and goats from the endemic areas of Khyber Pakhtunkhwa Province, Pakistan during 2016. The isolates were initially identified through colony characters, Gram staining and biochemical tests. The identifi...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ak and the adaptation of vaccine strategies. In spite of appropriate administration of vaccination, FAdV outbreaks have been reported and caused significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and gain a better understanding of the genetic properties of the FAdV strains responsible for recent outbreaks, the sequence analysis of hexon gene of 10 isolates associated with IBH and HPS was determined. Sequence alig...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...iv>Somatostatin (SS) DNA vaccine has been used to improve the growth and lactation of animals, however, the immune response is still unsatisfactory, especially in large animals. The present study aimed to investigate whether tissue plasminogen activator (tPA) could be applied as signal peptide for enhancing the effect of somatostatin DNA vaccine. A novel recombinant plasmid pEGS/2SS-tPA encoding two copies of somatost...
Hanif Ur Rahman1, Umer Saddique1, Zahoor-ul-Hassan1, Shakoor Ahmad1, Muhammad Kamal Shah1, Said Sajjad Ali Shah1, Farhan Anwar Khan1, Fazli Rabbani2, Muhammad Asif Hussain2, Attaur-Rahman2, Irshad Ahmad3,4 and Sadeeq ur Rahman2,*
... in the epidemiology and vaccine strategy against CCPP infection in small ruminants in the Northern region of Pakistan and therefore, a comprehensive country wide surveillance should further investigate the overall prevalence of members of MM cluster in suspected cases of CCPP in small ruminants.
...
G. Venkatesan* and Amit Kumar
...us that has been used as vaccine candidate for small pox infection in humans. This virus causes contagious form of disease, mainly but not limited to sheep and goats, which lead to high economic impact in developing countries including India. The causative agent, Orf virus (ORFV) belong to the genus Parapoxvirus (PPV) of the family poxviridae produces a typical papillomatous, cauliflower like external growth around mouth regions and rarely the lesions are foun...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...nd somatostatin (SS) DNA vaccine can enhance the effect of the vaccine and promote the growth of animals. This study investigated the enhancing effect of the fusion type of CpG motifs on SS DNA vaccine and growth of ram lambs. In this study, we constructed the ptS/2SS-asd plasmid, pCpG plasmid and the CpG-SS fusion type recombinant plasmid named ptCS/2SS-asd. Twelve ram lambs were r...
Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2
...ous biological controls, vaccines and local medicinal plants are being explored to control the parasitic worms. The aim of this study was to evaluate the anthelmintic potential of two local plants, Albizia lebbeck L. and Camellia sinensis L against H. contortus using Adult Motility Assay (AMA). Crude Ethanolic Extracts (CEE)of dried leaves of A. lebbeck L. and whole plant of C. sinensis L. were prepared by cold ma...
Omer Baris Ince*
... performed with inactive vaccines of suitable serotype and measures are taken in order to reduce the prevalence of the disease. In this study, blood samples were collected from clinically healthy sheep and bovine in the surrounding region of the southeast of Konya province that were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aim...
Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1
...ide the development of a vaccine may be required to reduce the risk of PPMV1 outbreaks. ...
Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1*
... Newcastle disease virus vaccines can provide various levels of protection against challenge with different NDV genotypes, raising the importance of the relationship between vaccines and field strains. The aim of the current work is to evaluate the effect of moisture percentage and pH on the persistence of velogenic NDV genotype VII in the poultry manure which considers a major threat to the Egyptian poultry industry since 2...
Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1*
... homologous/heterologous vaccines especially the live ones. The aim of the current work is to estimate the persistence time for velogenic Newcastle disease virus (NDV) of genotype VII in poultry manure in compare to LaSota vaccine strain as a model of genotype II. Velogenic NDV of genotype VII are a major threat to the Egyptian poultry industry since 2011 onwards with continuous evolution and diversity. In the present study,...
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
...tin 28 (SS-28)DNA vaccines in promoting lactation of rats. Fifteen female SD rats were randomly divided into three groups and were orally vaccinated, respectively SS-14 (Group T1) and SS-28 (Group T2) DNA vaccines fused tPA signal peptide and CpG adjuvant and delivered by attenuated Salmonella choleraesuis at weeks 0, 3 and 6 of the study, and rats in control group (Group C) w...
Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3
...racterized reference and vaccine strains worldwide. The IBD virus was detected in 9 out of 16 (56.25 %) investigated chicken flocks. Sequence analysis revealed that the analyzed Egyptian isolates identified as very virulent Infectious bursal disease virus (vvIBDV). The identity between these Egyptian isolates and the vaccine strains is ranged from 89.5%-95.6% and 88.1%-97.8% -at the nucleotide and amino acid sequence levels,...
Rana Waqas Arshad1, Asim Aslam1, Muhammad Saeed Imran1, Kamran Ashraf2 and Raheela Akhtar1,*
...e commercially available vaccines of infectious bursal disease (IBD). Three experimental groups, each having 100 broiler birds were placed in the same environmentally controlled house. Samples of blood and bursa of Fabricius were collected from slaughtered birds after every 7 days till the end of experiment (35 days). The size of bursas and the bursa/body weight ratios were significantly greater in live vector vaccine group ...
Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan
...class II of genotype II (vaccine strain) and 1 sample was phylogenetically related to NDV class II of genotype I with 98% identity. Furthermore, the intracerebral pathogenicity index (ICPI) for selected 5 virulent viruses reveals velogenic features with high pathogenicity index (1.60 to 1.74). ...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...iv>Currently a number of vaccines are under development against hepatitis C, though an effective hepatitis C vaccine is yet to come. The present study is focused on the development of recombinant vaccine against HCV by utilizing HCV3a envelope glycoprotein E2. HCV3a E2 gene was amplified and cloned first in cloning vector pTG19 and subcloned in expression vector pET21a. E2 protein, ...
Mustasim Famous*, Obaidul Islam, Shameema Khatun, Mohammed Mustafizur Rahman and Tania Ferdoushi
... only new-castle disease vaccine. Most of the turkey farmers rear turkey in small scale semi-intensive system (50%) was generally practiced by the rural farmers. They have very little idea about breed and variety of turkey and prevention of diseases of turkey. But farmers have very little knowledge about turkey management procedure. There is no specific feeding standard for turkey. Farmers used to feed their turkey according to broiler and layer feeding manual...
Mumtaz Ali Khan1,*, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Ikramul Haq4, Kashif Prince1, Asad Ullah5, Muhammad Shoaib2, Shahid Zaman6, Amjad Islam Aqib7, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4,Imad Khan5, Naimat Ullah4 and Muhammad Shahid8
...ated with enterotoxaemia vaccine. Group C was kept as negative control in both rabbits and goats. The immune titer against C. perfringens type D was evaluated on 0, 7, 14, 28th and 60th day of vaccination with indirect haemagglutination test (IHA). The results showed significantly higher (P< 0.05) immune titer in vitamin E supplemented animals groups against C. perfringens type D epsilon toxin.
...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...isolation of MAP to make vaccine against paratuberculosis using local isolates.
...
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
...y due to the lack of HCV vaccine. HCV genotypes are helpful for analyzing disease severity and start of therapy. Therefore, this study was designed to determine the genotyping of clinically isolated HCV from a Liver Center Faisalabad, Pakistan. A total of 3200 clinical samples were screened for the presence of anti-HCV antibodies by ICT method. Among positive samples, confirmation was carried out by RT-PCR and clustered into four groups (acute, chronic, cirrho...
Detection and Phylogeny of Infectious Bursal Disease Virus (IBDV) during Field Outbreaks in Broilers
Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1, Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8
...homology with attenuated vaccine strains. Our study revealed exclusive circulation of vvIBDV and these evidences emphasize the need of further detailed and more systemic approaches to study IBDV distribution for the implementation of effective control measures.
...
Naglaa M. Hagag1*, Mervat E. Hamdy1, Mary A. Sargious1, Sara M. Elnomrosy1, Nahed A. Ahmed1, Ayman A. Hamed1, Ahmed R. Habashi1, Essam I. Ibrahiem1, Mahmoud A. Abdel-Hakim2 and Momtaz A. Shahein1
...vaccination. Because the vaccine strains should match those strains circulating in the field, an updated vaccine is required to control the disease. Monitoring of FMDV in neighboring countries is essential to predict those strains that might escape into Egypt. ...
Yan-Guo Han, Yu-Qing Han, Jia-Yuan Wu, Jia Guo, Ri-Su Na, Yan Zeng, Guang-Xin E, Yong-Ju Zhao and Yong-Fu Huang*
...of somatostatin (SS) DNA vaccine in maternal animals on serum related hormones in offspring. In this study, ten ewes with two newborn lambs were randomly divided into SS DNA vaccine group (Group T) and empty vector group (Group C), and these vaccines were given orally into the ewes at weeks 0, 3 and 6 of the research. Blood samples of ewes or offspring lambs were collected at week 6 or 9 o...
Anand Kushwaha, Amit Kumar, Aparna Madhavan, Durga Goswami, Golmei Poulinlu and Gnanavel Venkatesan*
...ssays and also effective vaccine candidates which are vital elements in control and eradication of any infectious disease from an endemic country. In this review, we describe different structures of mature pox virion with reference to vaccinia virus (VACV), list of immunogenic candidate genes presents in CaPV genome and their potential use in diagnostic/vaccine developments. ...
Housh Muhammad Solangi1, Javaid Ali Gadahi1*, Mansoor Tariq2, Bachal Bhutto1, Zubair Ahmed Laghari1, Jamila Soomro3, Taufeeq Ahhmed Khosa1 and Abdullah G Arijo1
...unity and can be used as vaccine against the infection. However the results of FECRT indicated that HcCP produced partial immunity against the infection.
...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
...njected KISS1 DNA vaccines on immunocastration and in inhibiting mutton odour of male goats. In this study, 20 male goats aged eight weeks were randomly divided into the oral and plasmid-injected KISS1. DNA vaccine groups (groups T1 and T2) and the empty vector groups (groups C1 and C2) for immunisation. Blood samples were collected at 0, 3, 6, 10 and 14 weeks after primary immunisation for the detection of kis...
Adewumi Olubusuyi Moses1,2*, Faleye Temitope Oluwasegun Cephas,1,3, Ope-Ewe Oludayo Oluwaseyi1, Ibitoye Ibipeju Henrietta1, Arowosaye Abiola Opeyemi1, Adelowo Oluwatosin Deji1 and Adeniji Johnson Adekunle1,2,4
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
...ted as antigens and even vaccine candidate proteins in ticks or other pathogens but not in H. longicornis. Few reports have described dual specificity phosphatase 3 (DUSP-3) in parasites as an antigen. We successfully screened four potential antigens and found that DUSP-3 is a potential novel antigen for ticks.
... 
