Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...fy;">Oxidative damage to sperm during cryopreservation is one of the main causes of decline in fertilizing potential. Present study aimed at evaluating the role of melatonin in extender for its cryoprotective effect on post-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ...
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
...ole in the regulation of spermatogenesis acting primarily at the hypothalamic level of the gonadotropic axis. Citric acid is synthesized and secreted by the prostate gland. Its synthesis is regulated by androgens. As, dose dependent degeneration of prostate gland has been described following intraperitoneal kisspeptin treatment. However, effects of kisspeptin administration on the levels of prostatic citric acid remain elusive till date. The present study, the...
Muhammad Afzal1,Naveeda Akhtar Qureshi1,*, Khalid Zamir Rasib2 and Irfan Hussain1
...ricana and B. monosperma were most palatable for H. indicola. Feeding tendency in descending order of resistance based on mean mass loss as a quantitative parameter was recorded as: D. sissoo, S. cumini, P. roxburghii, J. mimosifolia, B. variegata, M. indica, A. lebbeck, A. indica, B. monosperma, P. euramericana.
...
Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
...nment. We found that the sperm-specific lactate dehydrogenase (LDH-C4) gene Ldh-c is expressed in plateau pika skeletal muscles. In order to shed light on the effect of LDH-C4 on the anaerobic glycolysis in plateau pika skeletal muscle, 20 pikas were randomly divided into two groups the inhibitor group (experimental) and the control group, reach of 10 pikas. The pikas of experimental group were injected with 1 mL of 1 mol/L N
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...teristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response ...
Shrinivas Mudigoudra and Shekharappa
Evaluation of plant products aganist sorghum shoot fly, Atherigona soccata Rondani
..., Vinca rosea, Butea monosperma, Aloevera @ 5% concentration and were compared with carbofuran 3 G @ 30 kg. ha-1 to know its efficacy on shoot fly in sorghum ecosystem. The results clearly indicated that NSKE (5%) recorded less eggs of shoot fly (0.40egg/plant), per cent dead heart (14.97%) and maximum grain yield of 13.22 q.ha-1 among different plant products. The next best plant product was found to be V. negundo @ 5% in recording less incidence of shoot fly...
Sogolo L. Lebelo1,* and Gerhard van der Horst2
...ound associated with the spermatogonia type B, pachytene spermatocytes and Sertoli cells. The results from this study show that there are basic similarities in the Leydig cells of the vervet compared to other mammals, humans and nonhuman primates, however, there also seem to be species differences among the mammalian group. The association of these cells with developing cells, and Sertoli cells was established. However, the ...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...v>The integral genome of sperm is crucial for successful fertilization and embryonic development. Different techniques have been used to determine the integrity of DNA in which acridine orange (AO) assay is easy and less costly. Though used in many species but the variability exists in technique. Therefore, the present study was aimed to optimize the steps of AO staining protocol for buffalo sperm which has not been explored...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...uation was performed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm characteristics such as sperm motility, viability, morphology, membrane integrity, and acrosome integrity were decreased in treated groups. DHA concentration was increased in all supplemented groups. Whereas ALA decreased i...
LX Ye1,2, JX Wang1,2,*, P Li1,2 and XT Zhang1,2
...compartments were mainly spermatogenic cells and some Sertoli’s cells. In the insterstitial tissue, the ghrelin-ip cells were identified as Leydig cells. The ghrelin-ip cells showed cytoplasmic staining, and were irregularly shaped. These findings indicate that ghrelin may participate in the spermatogenesis in the African ostrich. Further studies into this regulation will help clarify the physiological roles played by ...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...semen parameters such as sperm volume, motility, morphology, viability, and concentration. In conclusion, gradation of electrical stimulation into three stages (our modified Method II) could help to ease the collection of semen samples from bulls with minimum discomfort signs. Furthermore, the modified method is also recommended to use for other animals, in particular, the wild animals.
...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...ity, seminal pH and dead sperm percentage in fresh and thawed semen. Data were subjected to one way analysis of variance. High sperm individual motility (85.12 ± 0.51%), progressive motility (20.25 ± 0.47%), plasma membrane integrity (82.25 ± 0.43%), acrosomal integrity (79.91 ± 0.51%), and DNA integrity (98.54 ± 0.14%) were observed in fresh semen in Sahiwal bulls, which were significantly...
Ümit Kumbıçak, Zübeyde Kumbıçak* and Gülnare Huseynli
...e first time. Results of spermatogonial metaphases showed that the chromosome number and the karyotype formula of the species was 2n♂=35 (X1X2X30) which is the first record for the family Hersiliidae. Both autosomes and sex chromosomes were acrocentric. The lengths of the chromosomes indicated no significant difference between autosomal pairs, however, X3 was the smallest chromosome but X1 and X...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...ve in the development of sperm. Meanwhile, DUSP4, PDK4 and FKBP1B were also found to participate in testis development and spermatogenesis. In this study, online software and luciferase reporter gene system were used to predict and verify the target relationship between miR-122 and DUSP4/PDK4, miR-449a and FKBP1B. Thereafter, comparison analysis was done on miR-122, miR-449a, DUSP...
Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*
...e molecular mechanism of sperm storage.
... 
SHARIF UDDIN, MD. HASIBUL HASAN, MOHAMMED MAHBUB IQBAL, MOHAMMAD AMZAD HOSSAIN
... on matured testes found spermatocytes, spermatids and spermatozoa stages during the month of June and July.
Irfan1,*, Arshad Javid7, Muhammad Ashraf2, Athar Mahmud3, Muhammad Altaf4, Syed Makhdoom Hussain5, Muhammad Shahbaz4 and Khalid Javed Iqbal6
...ale birds. Mean abnormal spermatozoa rate was 14.61±1.61% with most common acrosome defects 39.67±3.80 % followed by defects in mid-piece 29.61±0.24%, head defects 10.15±1.21% and tail defects 20.57±1.97%. Laid eggs were collected, stored and were incubated for hatching. Hatchability percentage in artificially inseminated hens was greater 88.30% than natural mating 80.51%.
... 
Md. Nazirul Islam*, A. K. Azad**, Leon O. Namuco***, Teresita H. Borromeo***, Maria Lourdes O. Cedo*** and Edna A. Aguilar***
MORPHOMETRIC CHARACTERIZATION AND DIVERSITY ANALYSIS OF A MAKAPUNO COCONUT POPULATION IN U.P. LOS BANOS
...kapuno coconut whose endosperm is thicker and softer instead of hard crispy, growing at the University of the Philippines, Los Baños, was assessed using morphometric characters from March 2004 to October 2005. Shannon 2 Weaver Diversity Index and Chi-square (÷ ) tests were used to analyze variation in both the qualitative and quantitative characters. Substantial variation in crown shape, bole category, color of young fruit, petiole and infloresce...
Filiz Kutluyer*
...tryptophan on quality of sperm and its fertilizing capacity in endangered trout Salmo coruhensis, Anatolian trout Salmo rizeensis and rainbow trout Oncorhynchus mykiss. Different activation media (NaCl, 0.3%; NaHCO3, 1%) were supplemented with L-tryptophan [Control (0), 0.5, 1, 2, 3, 4 and 5 mM] for assessing sperm motility and its duration, fertility and hatching rate of eggs. The results fro...
María Magdalena Crosby-Galván1, Bernardino Espinoza-Velasco1 and Mónica Ramirez-Mella2,*
...pkin (Cucurbita argyrosperma) residue in ruminal digestibility and gas production in an in vitro assay. Four treatments were evaluated: a control using a standard diet for ruminants elaborated with corn stover, corn grain and soybean meal, and three experimental diets with 10, 20 and 30% dry residue of chihua pumpkin instead of corn stover. Dry matter digestibility and gas production was determined at 24 h of incubation in ruminal fluid obtained ...
Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene
Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*
...xpressed gene in chicken sperm storage tubules. In this work, Immunocytochemistry, immunofluorescence and Western-blot were performed to detect the expression of neurexophilin 1 in epithelial cells of chicken utero-vaginal junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells. The immunocytochemistry results showed that neurexophilin 1 was expressed in both the epithelial cells of chicken genital tract and DF-1 cells. Immunofluorescence revealed tha...
Souheila Slimani1,*, Sonia Hamouda1, Chahrazed Souadi1, Sara Silini2, Cherif Abdennour2 and Leila Delimi3
... 10. Consequently, an azospermia and lack of germ cells in control birds was noticed,which confirm the testicular regression.In the treated groups, thiram delayed the refractory phase along the experimental period, but testicular weight were superior in the treated pigeons compared to the control during the last weeks. Treated pigeons had more dead spermatozoa compared to the control. Remarkably, abnormal
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
..., membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination, pH was determined by digital pH meter. Motility was determined under microscope at10x magnification, morphology and live dead ratio was assessed using eosin nigrosin stain under 40x magnification...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
...n Jersey bull while live sperm percentage was significantly (P<0.05) high in Jersey bulls and sperm abnormality was significantly (P<0.05) high in Sahiwal and Friesian-Sahiwal cross. In addition, semen quality was improved in all the breeds after supplementation of vitamin C. There was no significant effect of BCS on the sperm quality of bulls. The results give important information ...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...iv>...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...ey by testing indices of sperm motility, mitochondrial activity, membrane integrity and arosome integrity. The results illustrated that compared with controls, above-mentioned indices of sperm were significantly improved under concentrations of Vitamin C (400 mg/L) and Vitamin E (600 mg/L) (P < 0.05). Moreover, physiological indices of donkey semen were significantly higher than the control groups (P < 0....
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
...amoeba with Trachyspermum ammi essential oil that have the ability to kill cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48 h) was more active, being able to kill 100% of the Acanthamoeba cysts.The results showed the cysticidal activity of T. ammi essential oil, LC
Saeed Murtaza1,*, Nazir Ahmad2, Muhammad Asif Raza3, Muhammad Saleem Akhtar1, Muhammad Mazhar Ayaz4, Muhammad Ali5 and Rais Ahmed6
...various lesions included sperm granulomata in the epididymis, testicular degeneration with or without mineralization, testicular abscesses and chronic epididymitis. In early stages, sperm granuloma appeared as an anechoic mass with ill-defined border. In advanced stages, it exhibited a mixture of anechoic and hyperechoic areas, encircled by a thick hyperechoic border. In early cases of testicular degeneration, testicular par...
Khalid P. Loneand Irfana Liaqat*
...on and regression of the spermatogenesis. This detailed study is the first from the Northern latitudes of the Indo-Pak subcontinent on the fish kept in commercial ponds.
...
Naimat Ullah1, Sarzamin Khan2, Muhammad Mobashar3, Shahbaz Javaid4*, Tarique Tonio1 and Asif Mahmood5
... quality parameters i.e. sperm motility and progressive sperm motility (%), sperm concentration (million/ml), live to dead ratio of sperm, amplitude of lateral head displacement of sperm (µm) and straightness of sperm (%) were measured at interval of 15 days. The results showed...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...otility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-cysteine supplemented groups in comparison with 0.5 mM or 0 mM. The number of supra-vital hypo-osmotic swelling test positive spermatozoa at 1.5 mM and 1.0 mM was greater in comparison with lower concentration of 0.5 or 0 mM L-cysteine. Furthermore, significant improvement for acrosomal integrity was recorded in semen samples treated with 1...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
...sticular observation and spermatogenesis evaluation. PFOS ≥ 11 mg/kg (accumulative dosage) resulted in visible histopathological changing in testis, including disorder and vacuolization of spermatogenic cells. Changed testicular organ coefficient (for dosage ≥ 55 mg/kg), decreased sperm concentration (for 110 mg/kg dosage), decreased motility (for 110 mg/kg dosage) and increased
Muhammad Khalid Rafique1, Rashid Mahmood1,*, Ziyad Abdul Qadir1,Imran Bodlah2 andFarid AsifShaheen2
...er treatments. The queen spermatheca diameter, emergence rate and mating rate were not found to be affected by the treatments and rearing interludes. Additional research is suggested and needed for clear recommendation.
...
Riaz Hussain Pasha1*, Muhammad Zubair Anjum2, Imran Ullah2, Muhammad Akram Khan3, Adnan Ali4, Saif-Ur-Rehman5, Sana Batool2 and Arslan Emmanuel2
...nica albuginea and rapid spermatogenesis in testes while appearance of cortical alveoli or yolk vesicle in cortex of cytoplasm in ovaries. Gonads attain maximum size and weight in spawning phase during summer. Free oozing of spermatozoa in testes and ovaries packed with fully grown eggs are the distinct features of this phase. The highest values of Gonadosomatic Index (GSI) for male and female in summer coincided with the hi...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...ikely noxious effects on spermatogenesis in mammals. Forty mice, randomly categorized into four groups (N=10) were administered orally with different concentrations of levofloxacin (0.00, 9.37, 18.75 and 37.50µg/g BW of mice) for 30 days consecutively. Mice were sacrificed on 31st day (24 hours after last dose administered) under deep chloroform anaesthesia. Blood was extracted through cardiac puncture and viscera were dissected out for furthe...
Amit Sharma1* and Pankaj Sood2
...functional damage to the spermatozoa leading to reduced motility, viability, impaired transport and fertility. Caprine sperms are extremely sensitive to cryopreservation compared to other species which is mainly attributed to the compositional variation of the sperm plasma membrane. Different factors affect affects the semen cryopreservation viz. species, breed, semen collection technique...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
... of frozen-thawed bovine spermatozoa. In this study, ten Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buserelin ...
Tingting Li, Shuangshuang Geng, Long Xie, Huiyan Xu, Aolin Luo, Pengwei Zhao, Huan Yang, XingWei Liang, YangQing Lu, XiaoGan Yang* and KeHuan Lu*
...d in the reproduction of spermatogonial stem cells (SSCs). To date, commercialized murine and human GDNF have typically been used for the in vitro culture of SSCs from domestic animals. However, whether endogenous GDNF is superior to xenogenic GDNF in SSC culture in vitro remains unclear. To answer this question, the buffalo GDNF gene was cloned and integrated into the PiggyBac eukaryotic expression vector, which was stably expressed in Sertoli c...
Paul Oludare Adetunji* and Safiriyu Idowu Ola
...parameters (age at first sperm cell and puberty) from 16-22 weeks of age, semen quality parameters (semen volume, semen concentration, sperm output, sperm motility and sperm abnormalities) from 26-52 weeks of age and sexual ornaments’ morphometry (Comb Length-CL, Comb Height-CH, Wattle Length-WL, Wattle Height-WH and Inter Pubic Distance-IPD) from ...
A. S. M. El-Nuby1† , S. A. Montasser2 and I. A. El-Khadrawy
Control of root-knot nematodes using wild plants colonized Sinai, Egypt
...rtemisia judaica, A. monosperma, Bassia muricata, Cornulaca monacantha, Salsola kali and Zygophyllum album with different dilutions (25%, 50%, 75% &100%). The potent nematicidal efficacy observed in the extract of A. judaica followed by A. monosperma. In vivo trial post inoculation treatments were effective than pre one, the maximum reduction in nematode population was recorded by A. judaica (87.0%) followed by A. mono
Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1
Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
...ber of frozen-thawed ram sperm (100 × 106 and 50 × 106 sperm dose) on pregnancy and lambing rate following laparoscopic artificial insemination (LAP-AI) in indigenous ewes in Bangladesh were studied. A total of 78 non- pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; G1 (n = 18; commercial Trilad...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...s; 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at 4°C, plunged and stored in liquid nitrogen (-196°C). Semen straws were rewarmed at 37°C for 30 seconds and ...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...of cryopreserved buffalo spermatozoa by addition of arachidic acid in semen extender. For this purpose, semen was collected from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 ...
Taqiur Rahman1, Tabassum Yaseen1*, Ali Mujtaba Shah2, Samiullah3, Ghulam Jelani3, Gul Nawaz1,Qudratullah Kalwar2
...to different families of spermatophytes have been collected, among which forty-eight belonged to angiosperm families and 2 families were gymnosperms. This comprises 39 herb, 14 shrubs, and 16 trees, respectively. The study postulated on the certification of herbal knowledge on realistic bases though former interviews for the documentation of accurate indigenous knowledge. (R1andR2) All the...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
... effect and mechanism of spermidine have been reported, the role of spermidine-induced autophay in cancer cell survival has not been fully elucidated yet. In the present study, we aim to investigate the effect and toxic mechanism of spermidine on cell survival using Hela cells, a cervical cancer cell line as a model. Cell viability was assessed by MTT assay. LDH leakage was determined usin...
Fan Sigang1, Guo Yihui1* and Xu Youhou2
...male organ formation and spermiogenesis were enriched in the testis. The glycosphingolipid biosynthesis pathway was identified for the first time in a mollusc testis.The present study provides the first transcriptomic analysis of C. nobilis, which will help clarify the molecular mechanisms of gonadal maturation.
...
Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3
...testosterone release and spermatogenesis. Intraperitoneal kisspeptin administration was carried out in 05 weeks old prepubertal male Sprague Dawley rats, twice daily for 12 days, at three different dosage regimens: 10ρg, 1ηg and 1µg compared to control (saline-treated) rats. Effects on pituitary gonadotropins [Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH)], secretion of testosterone and sperma...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2, Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5, Hamayun Khan1 and Shabana Naz6*
...lity parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treat...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
... in semen concentration, sperm motility and useable spermatozoa/ejaculate before 32 wk of age (p>0.05). Feed quantity was correlated positively with sperm concentration and sperm motility. At 50 wk of age, higher sperm motility was observed in males fed with 135.5and 125.5 g/d diet than males fed with 115.5 g/d diet...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
Eman A. Al-Shahari1,2, Abdelhalim A. Alkhazendar3, Eman R. ElBealy4 and Abeer A. Alm-Eldeen3*
..., testosterone level and sperm count and viability. Seminiferous tubules diameters were 84.3 ± 5.6 µm and their epithelium heights were 21.2 ± 3.4 µm. Only spermatogonia that were positively reacted with proliferating cell nuclear antigen (PCNA). After quercetin administration, they showed an improvement in the previous biochemical parameters. Seminiferous tubule diameters were 105.2 ± 2.1 &m...
Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2
...cellular contents of the sperm cells are important in the spermatological studies. Since homogenization processes reveal the intracellular contents of the sperm cells, we aimed to examine the optimal sonication procedure and the protective effect of the antioxidants against sonication. Four merino rams (2–3 years old) were used for semen collection, and the ejaculates were pooled and...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...s) to semen diluent. The spermatozoa freeze-thawed quality were assessed and found that total motility, progressive motility, membrane functionality, acrosomal integrity, and viability percentage were significantly higher (P < 0.05) in the diluent groups treated with 7.5 mmol of L-cysteine or 4.8 mmol of vitamin E. Correspondingly the DNA integrity and mitochondrial membrane potential percentage were found significantly higher (P < 0.05) in...
Hagar E. Mohammed1, Ranwa A. Elrayess2 and Heba N. Gad El-Hak2*
...ions of Artemisia monospermia with Mentha piperita on male fertility.Treated rats with different doses of A. monosperma or M. piperita alone induced severe testes histopathological lesions and histochemical alteration associated with a reduction in epididymis weight, abnormality in the sperm indices and a decline in serum testosterone levels which may have a bad...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
...Freezing process reduces sperm quality due to membrane damage. Antioxidants protects the membrane of spermatozoa. This study aimed to observe the effects of Snakehead Fish (Channa striata [Bloch 1793]) Extract (SFE) supplementation in tris Egg-yolk extender on mortality and motility of spermatozoa of Limousin bull (Bos prim...
Atilla Yıldız
...environment that affects sperm survival and determines the ability of cows to become pregnant. Insemination based on cervical mucus characteristics may increase pregnancy rate. Therefore, this study’s aim is to evaluate the extent to which the scores specific to the changes in physical properties of cervical mucus predict the probability of conception at insemination time. Cervical mucus in 36 clinically healthy Holstein cows was evaluated scoring system...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...ility, oxidative stress, sperm membrane integrity, morphology, and acrosome integrity. Four stallions were nominated for AI in the current study. The best result from different concentrations of cysteine and ascorbic acid were used for AI. Thirty mares were divided into three groups of 10 mares each and were then inseminated using the frozen semen with the following extenders: HF-20 extender (0) as control, 0.5 cysteine, and 0.5 ascorbic acid. The level of oxi...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...s, the normal process of spermatogenesis and quality of semen. Moreover, metals can lead to decreased or no production of prostaglandin, seminal fluid and endocrinology of reproductive process and thus results in male infertility. It was also here attempted to characterize the drastic effects of heavy metals on male infertility, the hormone analysis and genetic determination of SRY gene as some of infertile human had smaller sized testis. Blood and seme...
Gulzar Ullah* and Gohar Ayub
...tration (193.95 nmol/g), spermidine concentration (154.59 nmol/g), spermine concentration (36.51 nmol/g), yield (20.14 t ha-1), early flowering (26.99 days) and lowest electrolyte leakage (48.87%) was observed in plants treated with 20 hours heat pretreatment duration as compared to other heat pretreatment durations and control. Concerning tomato varieties, number of flower clusters plant-1 (16.99), fruit set percentage (68....
Kai Jin1,2,3, Chen Chen 1,2,3, Xinyu Sun1,2,3, Caiye Zhu1,2,3, Mahmoud F. Ahmed1,2,3,4, Qisheng Zuo1,2,3, Jiuzhou Song4 and Bichun Li1,2,3*
...ting transgenic goats by sperm-mediated gene transfer (SMGT). The expression of SCD1 gene and fatty acid metabolism genes in goat mammary gland tissues and muscular tissues was aligned with GEO database, and tested by qRT-PCR. F1 transgenic mice were mated and then the target genes and protein expression level in F2 transgenic mice, as well as fat content in F1 and F2 transgenic mice muscle were examined. Succ...
Yu Yang1,2, Shengqiang Ye1, Lixia Wang1, Xing Chen1, Yunguo Qian1 and Ping Gong1,*
... was mainly expressed in spermatogonia nucleus of seminiferous tubules and had no expression in Leydig cells. It was indicated that the DMRT1 expression was positively correlated with the testes development of adult mule duck.
... 
Khlood G. Abdelkhalek*, Aly B. A. Badawy, Mohamed Fathi, Elshymaa A. Abdelnaby
...r roles in regulation of spermatogenesis with the attainment of buck sexual developmental capacity.
Keywords | Estradiol, LH, FSH, Resistive index, Testosterone
...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...zen buffalo bull’s spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The anti...
Wassan Mhammed Husain1*, Zainab Sattar Ali2
...® might help improve sperm function in carbon tetrachloride (CCL4) -treated and healthy adult rats. Twenty-four adult rats were randomly divided into four groups and given the following treatments for 28 days: G1: as a control, the animals were oral administration with DW. G2: healthy rats were given 0.035 mg/kg BW of Phitofert ® every day by gavage. G3: twice a week, they were given 500 mg/kg B.W. of CCL4 mixed with an equivalent volume of olive oil (...
Nacira Djabali1*, Souad Dougat2, Wahida Haddadi2 and Lamis Fadhli2
...l characteristics of the sperm, the weight and the histopathology of the testes were studied. The results obtained show a significant dose-dependent decrease in LH, FSH and testosterone in the treated groups compared to control. The administered solvent caused severe deterioration in sperm quality, decreased sperm concentration, mobility and vitality, with increased <...
Ali Ikhwan1*, Dian Indratmi1, Faridlotul Hasanah1, Manar Fayiz Mousa Atoum2,3 and Irum Iqrar4,5
... – Cleome rutidosperma Linn.) can be extracted and function as an organic fungicide for environment-friendly control. This research aimed to examine the type and concentration of purple cleome extract metabolites and understand their effectiveness in inhibiting C. capsici. Purple cleome leaf is extracted with 1:1 w/v absolute methanol and concentrated with 1:1 v/v cold absolute methanol, and then the supernatant is purified using a centrifu...
Nour El-Hoda Khayrat Hammad1*, Yousef Y. El-Seady3, Azza E. Hassan1, Sara T. Elazab2, Magdy S. Amer2
...ed elevation in abnormal sperm % and significant decrease in serum testosterone hormonal levels and spermatozoa (viability and motility) which accompanied by some histopathological damage in testes and epididymis. On a molecular basis, mRNA expression of CYP17A1 and LHR genes were significantly reduced in testicular tissues of FCZ group while aromatase gene was elevated. The administration of LIN oil after FCZ treatment mark...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...ng cryopreservation, the spermatozoa faces osmo-chemical, mechanical and thermal stresses, which are predominant at dilution, cooling, equilibration, freezing and thawing stages. They damage functional and morphological characteristics of sperms. Beside these exogenous stresses there is an oxidative stress which damages the spermatozoa endogenously. These stresses can be controlled with th...
Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1
...ualitative parameters of sperm (volume, motility, concentration, survival) of rams bred in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42...
Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1
...ualitative parameters of sperm (volume, motility, concentration, survival) of rams bred in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42...
Taghreed Mohamed Nabil1, Randa Mohamed Hassan1, HebatAllah Hamdy Mahmoud2, Mohamed Gomaa Tawfiek2, Usama Kamal Moawad1*
Xian Zhang1, Erjiang Lin1, Shizhe Hong1 and Zhengjie Zhu2*
...nvestigate the effect of spermidine/spermine-N(1)-acetyltransferase (SSAT) on the proliferation and apoptosis of prostate cancer LNCaP cells under hypoxic conditions. Prostate cancer LNCaP cells were transfected with the eukaryotic expression plasmid pcDNA3.1 (D)/V5-His-SSAT, and cultured at 1% oxygen concentration. Western blotting was used to detect expression of SSAT, Akt, GSK-3β and β-catenin proteins; and flow...
Luke Chukwudi Ali*, Nnanna Ephraim Ikeh, Bright Chigozie Amaefule, Amarachi Linda Obinna, Ndubuisi Samuel Machebe
...erm motility, sperm concentration, live and normal sperms in decreased with an increase in dietary DCSM whereas non-motile, dead and abnormal sperms increased with an increase in dietary DCSM. Testicular morphometric measurements and histomorphological evaluations were similar in all treatments (P>0.05). This investigation depicts that DCSM inclusion above 15% affected semen ...
Gamal Shawki1, Tawfek Mohamed Barakat1, Attia Mohamed Samy2, Ahmed Alhamed AL-Mejren2, Ayman Mesalam1*
...ecially in semen volume, sperm count, livability, and motility during June and July. Similarly, the length, width, height and volume of testes as well as the width of total scrotum were higher (P < 0.05) during August, July and September. Moreover, the number of mounts per ejaculation, reaction time, time to erection, ejaculation times and time to ejaculation were significantly lower during summer and spring seasons (P < 0.05). A positive correlation (P ...
Emtenan M. Hanafi1*, Fouad M. Tawfeek2, Walid S. El Nattat1, Moetazza M. Alshafei3, Seham S. Kassem3, Kawkab A. Ahmed4, Enas N. Danial5, Hagar Elbakry3, Hoda S. El-Sayed2
.../font>atozoa had low sperm viability and higher abnormalities than control group. Feeding the microcapsules (G2,G3) improved the animals’ health condition. Animals in G2 showed good spermatozoa profile while, those fed SP (G3) showed low sperm count and motility. Histopathological examination declared testicular degeneration in G1 and G3 and a high sperm<...
Atta Ullah*, Nasrullah Khan, Ataur Rahman and Rafi Ullah
...ifferent indigenous angiosperms and their products in Sheikh Buddin National Park. Information from 250 respondents (240 male, 10 females) was gathered randomly about plants’ medicinal and folk uses in the park. One hundred and sixteen plants species belonging to 99 genera and 50 families were recorded and identified with the help of available literature. Of these, monocots were represented by 17 species under 15 genera and 5 families, while dicots were ...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
...osurvival of beetal buck spermatozoa with the supplementation of Glutathione (GLU) and L-cysteine (CYS) in the extender. In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM...
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
...and Y-chromosome-bearing sperms separated by using albumin gradient and swim-up methods in Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumi...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2
...tics of Bali-polled bull sperm to identify reproductive potential. Semen collection of Bali-polled bull was conducted once a week using an artificial vagina. The semen was proceeded until the frozen semen. Parameters were motility, motility progressive, kinematics sperms, viability, abnormality membrane integrity, and acrosome integrity. The Bali-polled bull semen was analyzed by using CASA with the ...
Idrissa Sacko1, Madou Dao1, Souleymane Sanogo1,2*, B. Michel Orounladji2, Kaly Diakité1, Diakaridia Traore3, Mamadou D. Coulibaly1, Amadou B. Cisse1
...tility and live and dead sperm counts (spz) were measured by direct reading and electronic microscopy with a direct image transfer system on screen, respectively. Sperm concentration and total sperm count in ejaculate were determined by microscopy using single chamber Neubauer cell. Results show that ejaculate volume was influenced (p < 0.05) by individual (buck). Besides, individual an...
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
...ty, individual motility, spermatozoa concentration, viability, and libido. Data were analyzed using the ANOVA test. The results showed that the volume and pH of semen did not differ in all treatments, but treatment A showed cream color and thick consistency over treatments B and C. The mass motility and sperm concentration of treatment C with the dose and period of GnRH administration resulted in the lowest values significan...
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
...eased (P<0.05) in all sperm characteristics and biochemical activity of extender as compared to E0 extender through semen extender preservation. The best level of BBE is marked when added up to 1.5% in the E3 extender which improves all characteristics compared to E0 and other BBE extenders. Also, E3 indicated post-thawing sperm parameters as motility, live, normal, and integrity acrosome up to 47.50, 65.10, 65.66, and 69...
Sri Suharyati, Siswanto, Madi Hartono, Kusuma Adhianto*
...om various parameters of sperm quality. Good sperm quality is very influential on reproductive efficiency. Vitamin E plays a role in male fertility because it can increase testicular weight, sperm count or concentration, sperm motility, and vitality. L-carnitine is an amino acid compound and is able to provide energy so that it can help improve
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...p>...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...AQP7 gene may affect the sperm cells of Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentr...
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...ffects semen volume, pH, sperm concentration, total sperm, total motile sperm, and frozen semen production of Aceh cattle (p < 0.05). However, it was found that there was no significant change in the sperm motility, before freezing, post-thawing, and during recovery across age (p > 0.05). Semen volume, total sperm
Saleh M. Albarrak1*, Fahad S. Aldahmashi1,2, Abdulrhman A. Alrubayan1,2, Abdel Kader A. Zaki1,3
..., ELISA ...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
... quality of Sahiwal bull spermatozoa. Qualifying semen ejaculates [motility (>60%) volume (>4ml) and concentration (>0.5 billion/ml)] collected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was fi...
Thobela Louis Tyasi*, Innocent Mokhwee Mohlabeng and Lebelo Joyceline Selala
...se animals with suitable sperm production for breeding. The objective of the study was to examine the relationship between body condition score (BCS), body weight (BW) and testicular measurements viz testicular length (TL), testicular diameter (TD) and scrotum circumference (SC). A total of 40 Dorper rams aged between 1 to 5 years were used for data collection. Pearson’s correlation was used to determine the relationship between BW, TL, TD and SC, and Sp...
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
...ditions adversely affect sperm function and quality traits of rabbit bucks. This study amid to investigate the efficacy of propolis ethanolic extract (PEE) oral administration on antioxidant capacity, reproductive hormones, immunity response, and quality and fertility of fresh and cryopreserved rabbit buck semen under HS conditions. A total of 40 APRI line rabbit bucks (7months old and 3.00±0.20 kg live body weight) were divided into four treatments (10...
Shama Sadaf1*, Komal Hassan1, Ayesha Saeed1 and Zeeshan Ahmad2
...achata indica, Butea monosperma and Litchi chinensis plants and applied on 100% cotton. Before and after applying antimicrobial finish mechanical property was checked. The antimicrobial finish was applied by pad dry cure method and finish was fixed by using of poly urethane binder. The presence of microorganisms was checked by ASTEM E2149 shake flask method before and after applying antimicrobial finish and after successive 25 washes. The results were analyzed...
Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*
...using a reduction in the sperm count and motility with a parallel reduction in the acid phosphatase activity. It also directly reduced the concentration of free testosterone and estradiol in both sexes without any apparent effect on LH or FSH hormones. It increased the total cholesterol in males only and elevated the triacylglycerols and glucose levels in both sexes. The administration of testosterone to males, females, or both reduced the fertility index to z...
Ibrahim Samir Abd El-Hamid1, Alaa Emara Rabee2, Moustafa Mohamed M. A. Ghandour2, Rasha Salah Mohammed3, Ahmed Mohamed. Sallam4
...≤0.05) mean values of sperm concentration, mass motility, sperm motility, sperm viability, and cell membrane integrity. Plasma testosterone concentration and number of red blood cells were higher (P≤0.05) in both treated groups. However alanine aminotransferase, cholesterol, total antioxidant capacity and glutathione peroxidase levels were reduced (P≤0.05) in both treated groups. ...
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
...trate-based extenders on spermatozoa quality of Brangus bull after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality.
Mustafa Erkan Özgür1*, Selim Erdogan2, Songül Aydemir3 and Hatice Yumusakbas2
...e relationships with the sperm cell velocities. According to our results, total levels of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for Salmo trutta fario occurred in higher quantities than Oncorhynchus mykiss but the quantities of total polyunsaturated fatty acids (PUFA) were less. Palmitic and stearic acid for SFA, oleic and nervonic acids for MUFA and linolenic, arachidonic, eicosapentaenoic and docosahexaenoic acids for PUFA of sem...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...ty of frozen-thawed bull sperm extended in Tris-buffered extender (TBE) supplemented with docosahexaenoic acid (DHA). Electro ejaculator was used to collect the semen and ejaculates having ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen samp...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
...ntain the quality of the sperm. This study aimed to determine additional honey to the quality of KUB rooster sperm during cold storage. The semen with superior quality was diluted into four different treatments, lactate ringer egg yolk without honey as control (LREY0), LREY with 0.2% honey (LREY2), LREY with 0.4% honey (LREY4), and LREY with 0.6% honey (LREY6). Semen was stored at 5°C for 48 hours. The...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
...squo; micromanipulation, sperm sexing, cryopreservation of ovarian tissues and germ cells and embryos, and genome resource banking for mammalian species. Such biotechnologies allow more offspring to be obtained from selected parents to increase milk and meat productivity, reduce the interval between generations, therapy of diseases and conservation of endangered mammalian species. Practically, current reproductive biotechnologies are species-specific because o...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
...ty and the proportion of spermatozoa X and Y from sexed semen using the Percoll Density Gradient Centrifugation method. The quality observed included individual motility, viability, abnormalities, concentration, total motile spermatozoa, chromatin damage, intact acrosome hood of spermatozoa, and sperm capacitation. The treatments consisted of T1 = unsexe...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
...f this study to identify sperms post-thawing quality and proteomic plasma semen as fertility performance in Bali-polled bull. The utilization of Bali-horned bulls as a point of reference was necessary to achieve this aim. The semen samples from Bali-horned and Bali-polled bulls were obtained twice weekly using an artificial vagina. The semen samples were immediately sent to the laboratory for processing. Parameters measured were sperm<...
Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi
...erved variables included spermatozoa motility, spermatozoa survival, spermatozoa abnormalities and intact plasma membranes of spermatozoa. The obtained data was analyzed using the Analysis of Variance (ANOVA). The results showed that the addition of tomato juice extract into egg yolk citrate diluent exhibited a 20% increase in sp...
Doaa H. Assar1, Rasha A. Al-Wakeel2, Zizy I. Elbialy3, Mahmoud M. El-Maghraby4, Helmy K. Zaghlool5, Adel A. El-Badawy4, Abdel-Khalek E. Abdel-Khalek6*
...lume, and percentages of sperm motility, livability, and abnormality as well as concentration and output of sperm cells were significantly by SPL. In serum and seminal plasma, glutathione, superoxide dismutase, catalase, and glutathione peroxidase significantly increased, while malondialdehyde significantly decreased in SPL groups. Based on our results, spirulina as a dietary feed additive (5 g/kg diet) could be used safely ...
Hasanain Jihad Neamah
...s known that ram’s sperms are sensitive to oxidative stress during cooling storage. The purpose of this study was the evaluation of adding an aqueous prickly pear extract (PPE) in ram semen on sperm parameters during cooling storage. Three levels of PPE were added to the diluted ram semen (PPE1=5mg +Tris extender, PPE2=10 mg +Tris extender, and PPE3=30 mg +Tris extender), and compared with control group (Tris extender ...
Galuh Chandra Agustina1*, Erwin Syahfitri1, Viski Fitri Hendrawan1, Siska Aditya1,2
...-3 and sertoli cells in spermatogenesis. A total of 20 male mouses were divided into five groups, K- (negative control, no treatment), K+ (positive control, 40 mg diazinon/kg BW); P1 (40 mg diazinon/kg BW + 200 mg black cumin/kg BW); P2 (40 mg diazinon/kg BW + 400 mg black cumin/kg BW); P3 (40 mg diazinon/kg BW + 600 mg black cumin/kg BW) throughout trials. The results showed that the black cumin seeds powder significantly decreased the caspase-3 expression, ...
Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago
...ation with variations in spermatozoa concentrations. Semen from 12 KBC was collected and evaluated to determine their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into 500µl of Ringer’s lactate solution with various concentrations of spermatozoa, namely 50×106, 100×106, and 150×106. Fertile period and fertility were evaluated...
Chun Shi, Juanjuan Guo, Meng Li, Qi Yang and Jingang Li*
Lalu Ahmad Zaenuri*, Rodiah Rodiah, Adji Santoso Dradjat, Oscar Yanuarianto, I Wayan Lanus Sumadiasa , Lukman Hy
...valuated for volume, pH, spermatozoa concentration/ml and concentration/ejaculates, plasma membrane integrity, viability, progressive motility, and morphology, including normal and abnormal spermatozoa. The statistical significance of the results was evaluated by pre- and post-test analyses using T-test with repeated measuring. Data were expressed as Means ± SD and SEM. Results revealed that semen volume,
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
...ion. Results showed that spermatozoa diluted with egg yolk citrate supplemented with 3% lycopene (P3) had better quality than that found in the other four treatments, namely motility before freezing (70.86%) and post thawing (53.00%); viability before freezing (76.00%) and post thawing (60. 14%); plasma membrane integrity before freezing (71.29%) and post thawing (59.86%); and also showed the lowest abnormality level before freezing and post-thawing, (17.29%) ...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
...ethane on the quality of spermatozoa. Bulls aged 3–4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4–5 0C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 1...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
... the longevity of canine sperm. To prolong the chilled canine sperm quality, the study investigated the effects of the adding antioxidants to semen extender on canine sperm during 12 days of chilling storage. Twenty ejaculates from five American Bully dogs were used. The sperm quality was determined by system automatic sperm
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
...>The preservation of dog sperm is impeded by oxidative stress, leading researchers to investigate the impact of incorporating essential oils derived from Ocimum gratissimum leaves in order to prolong the viability of chilled dog sperm. The sperm’s characteristics were assessed using an automated sperm analyzer and a confocal microscope to analyze m...
Khalid M. Al-Syaad1,2
...city (TAC) and number of spermatozoa compared with control group. The histological examination of the kidney revealed alterations induced by CC, including atrophy, tubular necrosis, enlargement of the glomeruli, dilatation and congestion of the glomerular capillaries (G) and the blood vessels around the renal tubules, atrophy in some renal glomeruli, hemorrhage, and around edema interstitial the blood vessels. It is concluded that utilizing propolis with CC co...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...buffalo semen to improve sperm characteristics and antioxidant capacity of sperm medium after cryopreservation. Tris-egg yolk extender with different levels (0, 4, 8, 12, and 14 mg/ml) of propolis ethanolic extract (PEE) were used. Semen was collected once/week for 20 weeks from five buffalo bulls aged 3.5–5 years. The collected semen was pooled and distributed into five equal fractions, each fraction was diluted with ...
Moustafa M. Zeitoun 1,3; Mohamed M. Mansour 2,4*
...en blood aldosterone and sperm motility percentage. Besides, plasma aldosterone levels in infertile animals were positively correlated (r = 0.26) with sperm abnormality ratios. Conclusion; The blood and seminal plasma aldosterone and testosterone levels could be used as a biomarker to predict fertility in male dromedary camels. Further, it could recommend measuring blood aldosterone relative to seminal plasma testosterone fo...
Omar Berkani1*, Souheila Slimani2, Nora Sakhraoui3 and Cherif Abdennour1
...ssion up to week 10. Azoospermia in control birds was noticed, with a significant increase in FT4 level and a fall of flying feathers in the 8th and 10th weeks, which assert the testicular regression. Compared to the control, CYP1 and CYP2 increased the testicular weights significantly during the last weeks by delaying the refractory phase until the end of the experimental period, while most of spermatozoa were malformed or ...
Zainab Sattar Ali* , Safa Azhar Razzaq , Layla Hammody Hashem
... observed damage in this sperms DNA and alteration of histopathological section. In addition reduction the levels of testosterone, inhibin b, FSH and LH hormones in group that ingestion BTSeNPs comparing the remnant groups, also been noted this reduction in above hormones getting worse with time progresses of experimental. In group4 show the propolis was enhancer factor for adverse effect of BTSe where FSH, LH, testosterone and inhibin b hormones values are el...
Wisit Ketpanyapong1, Kulisara Marupanthorn2*
... antioxidant status, and sperm quality in 24 Duroc boars at 1.0-1.5 years. The boars were randomly divided into four groups of three replicates each and their feed was supplemented with MOLE at concentrations of 0, 10, 50, and 250 mg/kg body weight (BW). Supplementing Duroc boras with MOLE increased leukocyte, erythrocyte, and hemoglobin levels, and significantly influenced serum antioxidant concentrations. The 50 mg/kg showed the highest serum antioxidant cap...
Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2
...idants on the quality of spermatozoa in chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4th day of observation showed that the additi...
Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2
...ty, and abnormalities of spermatozoa. The data obtained were analyzed using the analysis of variance (ANOVA) followed by Duncan’s multiple range test to determine the difference among the treatments. The current study showed that adding 15% sugarcane (T2) improved the semen parameters compared to the other treatments with 54% progressive motility, 74.8% viability, and 8.8% total abnormalities at 5°C for 4 days of storage. 15% sugarcane juice in egg y...
Amer Hakeem Chyad1, Omar S.I. Al-Janabi2*, Orooba Mohammed Saeed Ibrahim1, Najeeb Mohaamed Hussein3
...d all tested parameters (sperm count, total testosterone levels, FSH, and LH) compared to the CP group (P 0.05). Root extract of Chelidonium majus L. can be administered. Increase in all parameters and decrease in CP’s adverse effects on the testis.
Keywords | Herbal medicine, Biochemical indices, Chelidonium majus L. roots extract, Sperm count, Testosterone, ...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...ffalo was the quality of spermatozoa post-thawed. Therefore, research was conducted on the use of lycopene as an effort to improve the quality of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were ...
Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4
...zoa. ...
Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*
...n alternative to improve sperm quality. The difference in sample collection time after administration is assumed to have a positive effect on testosterone concentration. This research aimed to determine the effect of PGF2α injection and the optimal time for sample collection on improving semen quality and testosterone concentrations in Aceh bull. Aceh bull was used in this study and allotted into two treatments. In treatment 1 (P1), the bull received 25....
Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin2
...ther with the absence of spermatids, no epididymal sperm in the epididymal tubules. In conclusion, Folcord has a severe toxic effect on mice, as evidenced by histological and immunological changes in the male reproductive mice, according to the results of the current study.
Keywords | Spermatogenesis, Mice, Experimental design, Folcord, Oil adjuvant vaccine (...
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
... quality of Gaga chicken spermatozoa during liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitore...
Noor A. Namaa*, Hayder A.N. Al-Zamely
Hilwah Nora1,2, Rajuddin2*, Hafizuddin3, Rachmad Suhanda4, M. Fathurrahman5
...iminary studies based on spermatozoa quality parameters, morphology, and morphometry of testes and seminal vesicles that have never been reported. The main objective of this research is to examine the effects of curcumin on several aspects of reproduction and determine the optimal dose for using curcumin as an antifertility agent. The test animals in this study were Wistar rats (Rattus norvegicus) three-month-old males weighing 300 g. Rats were acclimatized fo...
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
... bestow superior quality sperm. Freezing and thawing rate are the most critical factor in the cryopreservation of milt. In the present study, variability between spermatological parameters of induced and non-induced C. carpio was documented for a period of 7 months. Further, the non-induced milt was stored for 24 h at three dilution ratio (1:10, 1:20 and 1:40) and spermatological parameter...
Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei
...pression also induce hypospermatogenisis in testes and sperm stasis in epididymis. So, the objective of the present study was to evaluate the toxic effect of tetrodotoxin on the histological structure of male reproductive system and oxidative stress induction in male albino rats. The methods included sixty male albino rats at age 8-9 weeks and weighted 190-200 gm were divided into three groups: The first group: Consisted of ...
Aziz Ahmed1*, Anila Naz Soomro2, Muhammad Ramzan Ali1, Muhammad Aleem Khan1 and Hasina Basharat1
...xt-align: justify;">Fish sperm-activating media are chemical solutions having certain properties now thought to be a prime indicator for the peculiarity of spermatozoa. Present research evaluated the effect of two laboratory-prepared media and one commercially available media i.e., ACTi fish while control is freshwater on sperm quality parameters i.e., percent motility, motility interval, ...
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
...and microscopically. The sperm used had a motility of over 80%. The semen was diluted Lactate Ringer with 10% egg yolk and the addition of 7% DMSO cryoprotectant. Then the semen was equilibrated at different times and then frozen. The frozen semen was then stored in a liquid nitrogen container at a temperature of -196°C.After 24 hours, the semen was thawing at a different temperature. The evaluation of semen quality included motility, viability, plasma mem...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...ative stress and affects sperm motility. Antioxidants in the diluent can maintain the motility of bull sperm during the cooling process until after thawing. This research examines the effect of Myrmecodia extract (MYE) supplementation, which has high antioxidants, on semen diluent during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) t...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...(chilled and frozen-thaw sperm traits) and in-vivo conception rate of Kamohri buck semen. ...
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