Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...l population of northern root knot nematode M. hapla and giving vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L. muscarium proved to be more effective against M. hapla whereas S. rugosoannulata and C. rosea showed better results among other tested fung...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...pulation dynamics of the root knot nematode, Meloidogyne incognita, and the nematophagous fungus, Lecanicillium muscarium, on tomato. The nematode population densities were measured by determining the number of galls and egg masses, juveniles and eggs per root system, and reproduction factor. Four initial populations (Pi) (500, 1000, 1500, and 2000 eggs) mixed with antagonistic fungal conidia levels (1×103, 1×104, 1×105, 1×106) were use...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...namide in the control of root knot nematode, Meloidogyne hapla, through soil application under greenhouse conditions. A significant (P=0.05) reduction in the number of galls and egg masses, eggs and juveniles (J2), and the reproduction factor (Rf=Pf/Pi) was observed in the plants treated with L. muscarium compared to the control. However, all chemical nematicides were also effective in reducing the nematode infestation significantly (P=0.05) in soil compared t...
Glomus mosseae (Gerd and Trappe) and Neemex Reduce Invasion and Development of Meloidogyne incognita
Atta ur Rehman Khan1,2*, Nazir Javed2, Shahbaz Talib Sahi2, Tariq Mukhtar1, Sajid Aleem Khan2 and Waqas Ashraf3
...ant parasitic nematodes, root knot nematodes are the major problem for vegetables including eggplant. Chemical control of nematodes is hazardous to health and causes environmental pollution by contaminating underground water. The bio-protectant potential of mycorrhizal fungus (Glomus mosseae) and neemex® (Azadirachtin) against invasion and development of Meloidogyne incognita was tested in eggplant roots in greenhouse pot trials. Neemex (5 g,...
Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6
.... The association of the root knot nematode species was identified based on observations on morphology of different life stages, beta-esterase phenotype and amplification of rDNA and partial sequence homology of ITS-1and ITS-2. Host differential tests confirmed the race 2 of M. incognita infecting passion fruit. Pathogenic relationship with the passion fruit was proved through inoculation studies and inoculated plants also produced almost similar above-...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...udied for suppression of root knot nematode, Meloidogyne incognita and wilt fungus, Fusarium oxysporum f. sp. lycopersici on tomato in laboratory and pot culture experiment. All the isolates were isolated by serial dilution and cultured in liquid protein supplemented broth medium and culture filtrates (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual cu...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...rentiating the important root knot nematodes species of India belonging to the genus Meloiodogyne. Further, molecular markers like RAPDs, AFLP, SSRs and PCR–RFLP of rDNA have been employed to study the molecular genetic diversity in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand t...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...ss the role of fungi and root knot nematodes in root rot disease complex of cotton and to optimize their control. For this purpose fifteen cotton growing areas in Southern Punjab were surveyed in year 2013-14. Data were recorded on disease prevalence, incidence, frequency percent of root rot fungi and nematodes population. Maximum disease prevalence was recorded in Tibba Sultanpur (100%) and Dunyapur (88%) whereas disease incidence calculated was 5% and 4.4%, ...
Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1
...lant pathogens including root knot nematodes (RKN). In the present studies, 15 isolates of Trichoderma harzianum were collected from different the root knot nematodes-infested tomato field of Malakand and Swat, Pakistan. These isolates were tested for their nematocidal effects against Meloidogyne javanica. Anti-J2 antagonistic test divided the isolates into 5 antagonistic groups or AGs. AG1 was observed as the most antagonis...
Matiyar Rahaman Khan1,*, Sabayasachi Pal2, Amit Singh2, Ashok Dhananjaybhai Patel3, Bhagubhai Ambaram Patel3, Tushar Manohar Ghule2 and Victor Phani1
...iv>Infestation of citrus root knot nematode (Meloidogyne indica) on the roots of citrus and Bt-cotton was found in Gujarat of India. The detection of M. indica and its diagnostic symptoms on some hosts (citrus and Bt-cotton) was recorded and the nematode species was authentically identified as Meloidogyne indica Whitehead, 1968 which was first time described on citrus from Delhi, India. The species is further characterized based on detaile...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
Sumaira Akram1, Sajid Aleem Khan1*, Nazir Javed1 and Saeed Ahmad2
Huma Abbas1*, Nazir Javed1,Muhammad Kamran2, Sajid Aleem Khan1, Hira Abbas3, Ehetisham-ul-Haq2, Abdul Jabbar1, Mehwish Naz1 and Ihsan Ullah4
Javeria Afzal, Muhammad Abid, Faisal Hussain* and Alia Abbas
