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Preliminary Development of an Enzyme-Linked Fluorescent DNA Aptamer-Magnetic Bead Sandwich Assay for Sensitive Detection of Rickettsia Cells

John G. Bruno¹*, Chien-Chung Chao^2,3, Zhiwen Zhang³, Wei-Mei Ching^2,3, Taylor Phillips¹, Allison Edge¹, Jeffery C. Sivils¹

Feasibility of Anti-Idiotypic Aptamers to Detect Rickettsia Exposure

John G. Bruno

...Rickettsia rickettsii and Rickettsia typhi. A feasibility study was conducted to generate anti-idiotypic aptamers against Fab fragments of these antibodies and to determine if the aptamers were capable of emulating rickettsial antigens to enable sensitive detection of rickettsia exposure in humans who have seroconverted. Such anti-idiotypic...

Amitava Konar, Palash Mondal and N. Johnson Singh

Occurrence of potato pests in different dates of planting in Gangetic plains of West Bengal, India

... soil pests such as molecricket, cutworm and potato tuber moth were found minimum in crops of 1st date of sowing resulted in producing highest number of healthy tubers. The rat damage was not so influenced by different dates of planting. But, the total tuber yield was obtained maximum in second date (4th week of November) which was closely followed by 1st, 3rd, 4th and 5th dates of sowing.

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Amitava Konar, Kiran A. More and Pradip Mondal

Efficacy of some insecticides against cutworm and molecricket of potato in West Bengal

...e: Lepidoptera) and molecricket (Gryllotalpa africana P. de Beau; Gryllotalpidae: Orthoptera) infesting potato at Adisaptagram Block Seed Farm, Mogra, Hooghly, West Bengal (India) during 2007-08 and 2008-09. The field trial was conducted in Randomized Block Design with six treatments and five replications. Among the various treatments for managing soil pests of potato (cv. Kufri Bahar) i.e. cutworm and molecricket soil treat...

Study of Rickets and Osteomalacia in Tibetan Gazelle

Bin Huo¹ and Xiaoyun Shen^1,2,3*

...le have been affected by rickets and osteomalacia, characterized by emaciation, growth retardation, lameness,enlargement of the costochondral junctions and abnormal curvature in long bones. The aim of the study was to determine possible relationships between the illness and mineral deficiency. The present result showed that phosphorus contents in soil and forage from affected pastures were significantly lower than those from healthy areas (P<0.01) an...

Prevalence of Anaplasma marginale in Tropical Area of Khyber Pakhtunkhwa, Pakistan

Saira Gul^1,2, Sohail Ahmed²*, Tahir Usman¹*, Khalid Khan³, Sultan Ayaz¹, Saleha Gul⁴and Nawab Ali⁵

...se caused by a rickettsial parasite Anaplasma marginale. This study investigated the prevalence of A. marginale in cattle on different commercial farms in subtropical conditions of Khyber Pakhtunkhwa (KP). A total of 120 cattle from different farms were randomly sampled and tested for the presence of A. marginale between May to August 2016. The study revealed that 15.0%, 20.8% and 29.1% of sampl...

Impact of Feeding Habits on Population Ecology and Breeding Biology of Grey Francolin (Francolinus pondicerianus) in District Bhakkar, Punjab, Pakistan

Aftab Raza Khan¹, Azhar Abbas Khan²*, Javed Iqbal³, Arif Muhammad Khan⁴, Zeshan Hassan², Hafiz Muhammad Aatif²and Umbreen Shahzad²

...including grasshoppers, crickets and locusts.

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Extended List of Orthoptera Fauna of Cholistan Desert (Punjab, Pakistan)

Santosh Kumar¹*, Riffat Sultana²* and Martin Husemann³

...pecies of grasshoppers, crickets and tree crickets. Caelifera were more diverse than Ensifera with 16 and 9 species, respectively. This work is provided an extended list of Orthopterans registered from Cholistan desert for the first time.

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BIOCONTROL OF BLACK CRICKET, GRYLLUS BIMACULATUS (ORTHOPTERA: GRYLLIDAE) NYMPHS WITH ENTOMOPATHOGENIC NEMATODES

A. N. Mahar, N. D. Janâ€ , A. Q. Mahar and S. R. Gowen

...control nymphs of black cricket Gryllus bimaculatus. Larvae (6

Investigation of Ehrlichia canis and Anaplasma platys from Dogs in Thailand, with Molecular Characterization and Haematological Profiles

Supawadee Piratae1*, Noraphat Khiewkham2, Nattawut Maungmungkun2, Chanakan Tippornwong2, Tossapol Seerintra2, Sirikanda Thanasuwan3, Luyen Thi Phung4

...and Anaplasma platys are rickettsial pathogens that cause infections in the blood and have adverse health effects in animals, especially dogs and other canids. PCR targeting the 16S rRNA gene was performed to determine the prevalence of ehrlichiosis and anaplasmosis in 127 dogs collected from Rayong province, Thailand. To confirm the identification of these two pathogens, PCR was performed to detect the citrate synthase (gltA) gene followed by sequencing. In a...

First Molecular Evidence of Anaplasma marginale Infection in Naturally Infected Cattle in Myanmar with Severe Hemolytic Anemia

Babi Kyi Soe¹*, Toe Win Naing², Su Lai Yee Mon¹, Nay Chi Nway³, Hiroshi Sato⁴

...e obligate intracellular rickettsial vector-borne pathogens that impact on livestock farmers with major economic constraints and eventually threaten human health in cases of zoonotic species involvement. Therefore, our study aimed to identify Anaplasma spp. infection in a bovine host using both microscopy and molecular techniques. A total of 59 samples were collected from Mingaladon township, Yangon region, and the presence of infection was assessed using a Gi...

Molecular Identification of Ixodid Tick Species and their Screening for Selected Protozoan Pathogens Collected from Large Ruminants of Azad Kashmir, Pakistan

Anisa Mushtaq¹, Murtaz ul Hasan¹*, Asim Shamim², Muhammad Ali Abdullah Shah¹, Muhmamad Arif Zafar³, Abdul Asim Farooq⁴, Aayesha Riaz¹, Muhammad Kamran¹ and Saif ur Rehman¹

... like viruses, bacteria, rickettsia and protozoa and cause mortality in humans and animals. This study was focused on morphological and molecular identification of ixodid ticks, using morphological keys and an internal transcribed spacer (ITS-2) Deoxyribonucleic acid (DNA). Moreover, the presence of Babesia and Theileria species was also investigated in ticks using 18S rRNA gene. Identification of ticks collected from 384 cattle and 384 buffaloes screened reve...