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James Ayukepi Ayukekbong1,2

E-mail | james.ayukekbong@microbio.gu.se

...e tropics and subtropics regions of the world. Dengue and malaria are endemic in Nigeria. Two independent studies among febrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever and the prevalence of dengue-malaria co-infection is high. Differential diagnosis of fever is therefore necessary to init...

Varadaraja V. Raman

...nocent civilians in many regions of the world. Governments have terrorized their own people, the most glaring instance was in post-Revolutionary France during the Reign of Terror. On the other hand disgruntled, oppressed, and frustrated citizens (Irish Catholics, Algerian Muslims, Sri Lankan Tamils, etc.) have terrorized people and governments for various causes.There has also been terrorism by the majority on the minority in communities and nations: for examp...

Gerald Misinzo1,*, Tebogo Kgotlele1, Epaphras A. Muse1, Jan Van Doorsselaere2, Mikael Berg3, and Muhammad Munir4

...disease, particularly in regions with uncontrolled livestock movements.

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Muhammad Shah Zaman1, Ghulam Muhammad Ali2, Aish Muhammad1, Khalid Farooq2, Iqbal Hussain1*

...ops in arid and semiarid regions. In vitro screening of eight potato varieties (Asterix, Cardinal, Challenger, Desiree, Hermis, Kroda, Sh-5 and Sante) was investigated for salt tolerance at 0.0, 10, 20, 40, 60, 80, 100 mM NaCl supplemented with MS medium. Kroda emerged as the most salt tolerant variety with the highest plant height (6.5 cm), number of nodes (8.8), fresh shoot weight plant-1 (0.166 g), number of roots (4.6) and root length (2.5 cm) at 60 mM Na...

Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2

...tributed in all analyzed regions (Central, Chorotega, Atlantic Huetar, North Huetar, and Central Pacific) determining regional seropositivity between 63.5% and 100.0%, as well as an overall prevalence of 80.8 %. The within flock seropositivity percentages ranged between 0% and 100.0%. Flocks with the highest seropositivity were found in low altitude regions close to the coast. Risk and protective factors determined in the pr...

Behzad Hussain1, 2, Abdullah Iqbal2, Muhammad Abubakar2*

...becoming endemic in many regions. The first case of CCHF was reported in 1976 in Rawalpindi and after that, multiple sporadic cases and outbreaks have occurred in subsequent years and till today. CCHF outbreaks have a case fatality rate of up to 40%, which is alarming. It is a zoonotic disease and the virus is transmitted mainly to the humans through hard tick bite and direct contact with blood of the infected animal. The occurrence of CCHF outbreak in Pakista...

Zuhao Huang1, Feiyun Tu2 and Dianhua Ke1*

...NA genes and two control regions CR and CCR, which was first reported in the order Coraciiformes. The overall A+T content for the mitogenome is 52%, and the GC and AT skews are -0.400 and 0.108. Unlike to many other birds, no extra base is inserted at certain position relative to ND3. Interestingly, a 484bp repeated sequence appears in both CR and CCR. Genetic distance shows that the differences between M. v. viridis and M. v. americanus is highe...

Irfan Ullah and Abbas Ullah Jan

...ls in the family in both regions. Prices of basic food items such as milk, meat, rice, other cereals, backed products and other food should be kept constant. Imposition of any sale tax could create huge loss in consumption for these commodities. The demand for most of food groups are own price inelastic in both regions except milk, rice, tea and coffee and backed products which are more than unit elastic to own price. Simila...

Sania Subhan Qureshi, Mian Saeed Sarwar and Zahir Shah

...awar and the surrounding regions. It provides a source of meat and eggs to the local population and helps in poverty alleviation and a source of family income through entrepreneurship. A hen with the complaint of swelling on abdominal region, came to Veterinary Teaching Hospital, The University of Agriculture Peshawar. Physical examination was performed and it was diagnosed a case of muscle tumor (myoma). A skin incision was given to expose and to remove tumo...

Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar

...ency of virus in endemic regions.

...
Serkan Sayiner1* and Hilal Karagul2
...evels in eight livestock regions of Northern Cyprus from two different seasons. We also evaluated whether these concentrations were adequate. Heifer blood, feed samples including concentrate feed or roughage were analysed. It was found that there are significant seasonal differences for each region and regional differences in each season were due to different feeding conditions. Heifer blood selenium and plasma vitamin E levels were generally found within adeq...
Zafar Iqbal1* and Muhammad Khurshid2 
...tropical and subtropical regions of the world. This is the first report of the use of heterologous polyclonal antibodies (PAbs), raised against a geographically distinct begomovirus, ToLCNDV. The described results have shown to exhibit a many fold increase detection sensitivity and reproducibility hence, can be adopted for routine detection of ToLCNDV from plant extracts.
...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3

 

...low as compared to other regions of Saudi Arabia.
...

 Saqib Ali1, Suliman Ali1, Lina1, Wen Zhou1, Muhammad Irfan Waris1, Ashfaq Ali2 and Man Qun Wang1,*

...the spread of ALB in the regions of China. The ecological, in addition to biological uniqueness and development of sawyer beetles (Monochamus alternates) are also discussed.
...

Sadiq Ali* and Ijaz Ahmad Khan

...vince, but more affected regions are Peshawar valley and Hazara division where it has enormously invaded most of the open spaces like roadsides, wasteland and water ways resulting in loss of local biodiversity. Field survey of district Swabi (Peshawar Valley) of Khyber Pakhtunkhwa, was carried out during May-June, 2013-14 to study the distribution of Parthenium weed. Five villages were selected and thirty locations were randomly selected from each village. The...
Abdus-Subhan1, Qudratullah Khan1, Muhammad Mansoor2, Muhammad Jamil Khan1, Ammanullah2, Matiullah Khan3
...recommended for the arid regions.
...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...nd appearing even in new regions, previously free from such diseases. Techniques for geminivirus detection include biological indexing, electron microscopy, antibody-based detection, including ELISA, nucleic acid hybridizations like dot blot, squash blot and Southern blot techniques, polymerase chain reaction and other DNA polymerase-mediated assays, and microarray detection. Of these, microarray detection provides the greatest capability for parallel yet spec...

Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram 

Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
...collected from different regions of Karnataka for isolation of chilli microflora. From these samples, 94 fungal and 89 bacterial isolates were derived, which included 50 isolates of fungi and 44 isolates of bacteria from phylloplane and pomoplane, 44 isolates of endophytic fungi and 45 of endophytic bacteria from inside the leaf and fruit tissues, respectively. Among them, 70 fungal isolates belonged to plant pathogenic genera like,Alternaria, Cercospora, Coll...
Jia-xiang Wang1,2,* and Peng Li1,2
...lets to the extrainsular regions. The distribution of ghrelin-ip cells was observed mostly atthe periphery of the islet, a few ghrelin-ip cells were found in the central portion of the pancreatic islets. The ghrelin-ip cells were fusiform or irregular polygons, and their cytoplasm was stained intensely. These results demonstrate the presence of ghrelin-ip cells in the pancreas of the African ostrich. Thus, it is speculated that ghrelin may have a physiological...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...d control groups. Exonic regions of BDNF gene were sequenced. In-silico analysis was performed to detect the possible disease causing effect of genetic variants with OCD. Sequencing of BDNF gene showed one change G>A at position c.196 (rs6265) resulting in a nonsynonymous change from valine to methionine. We found only one homozygous and heterozygous carrier for this polymorphism. Healthy siblings of the carrier individuals and control group w...
Sogolo L. Lebelo1,* and Gerhard van der Horst2
...atin and heterochromatin regions with the latter usually occupying the periphery of the organelle. The cytoplasm contains numerous mitochondria containing distinct lamellar cristae, small patches of rough endoplasmic reticulum as short cisternae, a large dense network of interconnecting tubular smooth endoplasmic reticulum, and round or oval lysosomes. Abundant lipid inclusions and collagen fibers are also found in the cytoplasm of these cells. These cells are...

Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4

...cid analysis of NA stalk regions of these isolates illustrated no insertion, deletion and substitutions compared to A/DK/HK/Y280/97 lineage and human isolates (A/HK/1073/99 and A/HK/1074/99). Several amino acid substitutions at various regions of the NA protein were determined with unknown functions and consequences. These findings warrant future studies for analysis of whole genome sequencing of prevailing H9N2 viruses in P...
Kanwer Shahzad Ahmed1, Muhammad Zeeshan Majeed1,*, Muhammad Ather Rafi2, Fatima Sellami3 and Muhammad Afzal1
...eetles in seven selected regions (Bhera, Bhalwal, Kot Momin, Sahiwal, Sargodha, Shahpur and Silanwali). A total of 1,470 coccinellid specimens were collected from all types of vegetation and were identified up to species level by means of external morphology, published descriptions and male genitalia micrometry. Nine species were identified belonging to 9 genera and 4 subfamilies occurring along with 10 trophic associations. Five species viz; Coccinella sep...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,
....0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged from two to five and the mean number of alleles was 3.67. The observed and expected heterozygosities ranged from 0.227 to 0.545 and 0.201 to 0.630, respectively. The polymorphic information content value ranged from 0.181 to 0.573. Null allele frequency varied from -0.0548 to 0.0563. The sets of loci proved to correctly discrimin...
Zuhao Huang*, Meifang Li, Rujue Ruan and Jiawei Qin
...es had duplicate control regions, except four species having only single region. The control region spans the region between the genes for tRNAGlu - tRNAPhe in the most Ardeidae species. The length of the control region sequences ranged from 1427 bp (Ixobrychus flavicollis) to 3871 bp (Botaurus stellaris). The average genetic distances among the species varied from 5.39% (between Egretta sacra and Egretta garzetta<...

Carolina Torres Alejo1,2, Laila Andreia Rodrigues Beserra1,2, Paulo Eduardo Brandão1,2, Luis Ramiro Luna Espinoza1,2 and Fabio Gregori1,2* 

...from different Brazilian regions for the presence of rotavirus (RVA, RVD, and RVF), avian coronavirus (ACoV), and avian astrovirus (AAstV), using PCR reactions followed by nucleotide sequencing and analysis. Twenty-nine pools (29/59; 49%) appeared positive for AAstV, 6 pools (6/59; 10%) were positive for RVD, forty-three pools (43/59; 72.8%) were positive for ACoV, and twenty-four pools (24/59; 40.67%) presented concomitant viral infections of two or three inv...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali

...S in the basal scutellar regions of immature embryos compared to apical and embryo axis region. Heat shock of 46 oC for 3 minutes increased the transformation efficiency of immature embryos by 26.96%. Growth of infected immature embryos on resting medium after co-cultivation period enhanced the survival of kanamycin resistant calli on selection medium by 93.97%. Agropine strain (EHA101) of Agrobacterium was found 25.67% more effective than octopine strain (LBA...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...ne 5’ and 3’ regions. Sequences of 5 fragments of SAG2 were annotated and analyzed using DNASTAR Lasergene. After phylogenetic analysis with 3 clonal types and atypical strains, and on the basis of restriction map of HhaI and Sau3AI, our 3 isolates of T. gondii were found more closely linked to a typical strain. This is the first genetic analysis of T. gondii in Pakistan. In order to develop our knowledge about the toxoplasmosis epide...
Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem
...loying 18S hypervariable regions as molecular markers, we show a sequence analysis of (V3, V4 and V9) hyper-variable regions of the small sub unit (SSU) of different natural strains of the Pyrenomycete, Sordaria fimicola, to examine genetic diversity. Strains were taken from two contrasting environments (Natural and Harsh) from the south facing and north facing slopes of Evolution Canyon, Israel. Sequenced amplicons w...
Nosheen Rafiq1, Asmatullah Kakar2,*, Arshad Ghani3, Asim Iqbal2, Wali Mohammad Achakzai2, Shagufta Sadozai2, Mohammad Shafiq4 and Mohammad Alam Mengal5 
...arm houses at three main regions (Western by-pass, Spiny village, and Sariab region) of District Quetta from March, 2013 to March, 2014.   Around 1649 ticks were captured from six main body parts of 404 cattle. Of these 346 (65.96%) were observed to be plagued by one or more tick infestation. Compositions of tick species captured from the host were Hyalomma anatolicum anatolicum (33.39%), followed by Dermacenter andersoni (31.76%), Hyalom...
Medeni Aykut
... South-eastern Anatolian regions of Turkey. A total of 3,990 dysticid specimens were collected from 118 different localities and identified. In total, 40 species in 17 genera of family Dytiscidae were recorded, of which 37 were new to the study area. Eretes sticticus (Linnaeus, 1767) is the second record for Turkish fauna. Furthermore, Hydroporus inscitus Sharp, 1882 is new record for the Turkish fauna.
...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
... there were no conserved regions flanking CcSox6. Numerous essential transcription factor binding sites (TFs) were predicted within the 2000 bp upstream of the 5’ end of these genes. These TFs include BSX, BRN4 and NGN–NEUROD, which have been shown to be involved in the early stages of neuronal determination and neurogenesis in vertebrates. Tissue distribution analyses by Quantitative real-time RT-PCR (qRT-PCR) revealed that CcSoxD ge...

Muhammad Naeem Safdar, Khalid Naseem, Muhammad Amjad, Amer Mumtaz and Saeeda Raza*

...cted from nine different regions of Punjab and analyzed for physicochemical characteristics during 2006 to 2008. The quality parameters studied were test weight, 1000 kernel weight, foreign matter (non-edible and edible), broken/shrunken grains, damaged grains, moisture, ash, protein (N x 5.7), wet and dry gluten, falling number, and minerals (copper, manganese, iron, zinc). It was observed that wheat samples varied considerably within each region and also bet...

  Abdul Hassan*, Muhammad Ishaq*, Nisar Ali Shah* and Arshad Farooq*

MILK PRODUCTION POTENTIAL IN KHYBER PAKHTUNKHWA
...in three agro-ecological regions, namely, barani areas, wet mountains and Northern dry mountains of Khyber Pakhtunkhwa, Pakistan by using concept of typical farms. The yield and production of milk gap was estimated in three scenarios. The results showed that the present milk production of Khyber Pakhtunkhwa is 2.74 mt with 1579 lit of milk per buffalo per year, 663 lit of milk per cow per year, 95 lit milk per goat per year and 64 lit of milk per sheep per yea...

 Naveed Jehan*, Khalid Mahmood Aujla**, Muhammad Shahzad*, Abid Hussain**, Muhammad Zahoor*, Majid Khan* and Ahmed Bilal*

USE OF MOBILE PHONES BY FARMING COMMUNITY AND ITS IMPACT ON VEGETABLE PRODUCTIVITY
...ous markets in different regions and can accordingly arrange transportation and labor services in time. The study was conducted to see the effect of timely information availability with mobile on productivity. The data was collected through well structured questionnaire by interviewing 60 farmers from district Charsadda. The data was analyzed through simple averages, correlation analysis and paired t-test. Average yield of bitter -1 gourd crop was 23569 kg acr...

 Anwar Hussain* and Muhammad Rahman**

ROLE OF TRAININGS ON FARMERS' PROFITABILITY OF MEDICINAL AND AROMATIC PLANTS IN MOUNTAINOUS AREAS OF DISTRICT SWAT, KHYBER PUKHTUNKHWA
...pe of trainings in other regions of the country which will not only increase the trade volume of the MAPs but would also improve the livelihood of the local community. 

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 Ahmed Fatima*

INCIDENCE OF POVERTY AND THE ROLE OF NON-FARM ACTIVITIES
...olds being better off in regions that are less fertile. The incidence of poverty was highest amongst farm wage workers, followed by non-farm wage workers, non-farm self-employed and lastly crop cultivators. 

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 Waqar Akhtar*, Muhammad Sharif**, Abdul Hayee Qureshi*, Khalid Mahmood Aujla*** and Muhammad Azeem Khan*

COMPETITIVENESS OF TOMATO PRODUCTION IN PUNJAB, PAKISTAN
...to two tomato production regions i.e., Central and Southern Punjab for analysis purpose under importable scenario by using import parity price. Results of PAM model revealed that tomato production in both regions of Punjab has competitiveness under prevailing market situation as indicated by positive private profitability and private cost ratio (PCR) which is less than 1. Competitiveness difference in two
Aitezaz Ahsan1*, Muhammad Usman1, Ihsan Ullah2, Aamer Bin Zahur3 and Adnan Rasheed Malik4
...oat, particularly in the regions of Africa, Middle East and Asia. Besides clinical signs and symptoms of PPR various diagnostic assays, both serological and molecular, have been developed and are applied for estimating the current status of the disease. This review article focuses on the development of different molecular diagnostic assays from conventional reverse transcriptase polymerase chain reaction to real time reverse transcriptase polymerase chain reac...
Muhammad Zeeshan Majeed1,2,*, Abu Bakar Muhammad Raza2, Muhammad Afzal2,Hafiz Salah-ud-Din2, Imtiaz Sarwar2, Muhammad Yahya2 and Khurram Shehzad3
...tropical and subtropical regions reported elsewhere. Therefore, keeping in view the key role of soil invertebrates in soil sustainability, it is recommended to mitigate the deleterious effects of land-management practices on these important soil biotic components.
...

Muhammad Jamal Khan1, Abdul Malik2*, Muhammad Shahzad Khattak2, Naveedullah1, Naeem Ijaz3 and Ishaq Ahmad4 

...ce in arid and semi-arid regions of the world. In recent past, its contamination due to lack of balance between its exploitation and recharge has posed a serious menace on its utilization for various purposes. Current study was conducted in District Karak during June to November, 2009 with the objectives to assess the changes in groundwater table depths due to withdrawal and the water quality parameters and to find the effect of small dams on recharge of groun...
Farhan Ahmad1,*, Muhammad Waris Sanjrani1, Shah Nawaz Khuhro1, Asif Sajjad2, Abid Ali3, Rashad Rasool Khan3, Farooq Ahmed3 and Junhe Liu4,*
...ds in all cotton growing regions. Cotton whitefly (Bemisia tabaci G.) being vector of CLCuV in Pakistani cotton crop is responsible to cause an economic damage. A survey of whitefly populations and its parasitism with connection to temperature and relative humidity was conducted in fourteen cotton growing districts of Sindh province (southern Pakistan) for 2012 and 2013 seasons. There was a significant difference in whitefly population and percent paras...
Sherin Reda Rouby
...neighboring, non-endemic regions with significant economic implication. Capripoxvirus isolates are extremely conserved with genome identities of at least 96% between SPPV, GTPV and LSDV. The LSDV is similar in antigenicity and in cultural characteristics to SPPV. The current study was delineated to identify capripoxviruses from different clinical samples and differentiate capripoxviruses without the need of gene sequencing. A total of 16 lumpy skin d...
Waqas Ali1,*, Arshad Javid1, Syed Mohsin Bhukhari1, Ali Hussain1, Syed Makhdoom Hussain2, Hira Rafique1
...fectiveness in different regions of the world are still debatable. Moreover, data recorded from inappropriate designed surveys are not suitable for statistical analysis and provide inappropriate picture regarding distribution, abundance and status of target species. Current review inspects different sampling techniques, various issues related to surveys design, application and ethical issues connected with these sampling techniques. It can be concluded from th...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...d species across various regions of the country. AIV remains a pandemic threat therefore vigilance for routine AIV surveillance programs and improved vaccination strategies are highly desirable.
...

Mahwish Rehman1*, Sajjad Ahmad1, Maqsood Shah1 and Inamullah Khan

... colonies from different regions of Khyber Pakhtunkhwa i.e. Peshawar, Mardan, Kohat and Chitral to discovered Varroa mite species. Identification was done by using key developed byDe-Guzman and Fernandez and Coineau, (2007), i.e. body size (length and width), location of seta, shape, size (i.e. bristle hair, stiff) on dorsal shield, chaetotaxy and structure of the sternal, , metapodal, anal, and epigynal shields, periterme, morphology of setae on the legs and ...
Baoying Guo*1, Yu Chen1, Chuan Zhang2, Zhenming Lv1, Kaida Xu3, Hongling Ping4 and Huilai Shi4
...es, and 2 long-noncoding regions [both in the control regions (CR)]. The composition and order of genes in S. lycidas were similar to those of most other invertebrates. The overall base composition of S. lycidas is 35.8% T, 14.8% C, 41.3% A, and 8.1% G, with extremely high A+T content (77.1%). Both control regions contain termination-associated sequences and conserved sequenc...

Ashraf Sayed Awaad1 and Mohamed Zaki Fathy2*  

...n seven lumbosacropelvic regions of goat cadavers and five live goats of both sexes ranged between 25-30 kg body weight. Longitudinal and cross anatomical sections were done on these samples; the sections were photographed and compared with coronal and sagittal sections of CT images. While transverse and parasagittal US were performed in goats over the selected new site (last inter-lumber space (L5-L6). The latter was compared with the L6-S space by gross anat...
Bo Wu1, Hui Zhang2, Kun Li2, Khalid Mehmood2,7, Yan Zhao1, Bin Jiang1, Chengjun Xue3, Muhammad Tariq Javed6, Fazul Nabi2, Zhaoqing Han5,* and Houqiang Luo4,*
...FMD is uncertain in many regions of China and many outbreaks are not reported. This study was undertaken to investigate the sero-prevalence and immunization program optimization of serotype O of FMD through ELISA and IHA in pigs in Zhejiang. A total of 368 serum samples were collected from June to July in 2016. Out of these samples, 23 (6.79%, 95% CI 4.4-9.9) pigs were found positive for FMD antibodies with the further distribution of 9.09% (95% CI 4.6–1...

G. Venkatesan* and Amit Kumar  

...rnal growth around mouth regions and rarely the lesions are found in other parts of the body and internal organs. Another characteristic feature of ORFV including other PPVs is the ability to re-infect the same host and conferring short term immunity in contrast to other pox viruses and also produce localized skin lesions in humans who are in contact with infected animals and their products. Unique and specific virulence genes located at termini of ORFV genome...
Ai Guo1,2,3, Jiaguang Xiao4, Binbin Shan4, Tianxiang Gao5 and Yongdong Zhou3,*
...ing genes and non-coding regions. The mitogenomes of C. mystus N and C. mystus S shared the identical structural organization and gene arrangement with those of other Coilia fishes. Both lineages of C. mystus showeda similar features in not only the strand-specific asymmetry of nucleotide composition, but also the codon usage of genes. Whereas a significant variation among Coilia species was detected in length of the control ...

Ghulam Abbas1, Khalid Pervez Akhtar1, Muhammad Ahsan2, Muhammad Jawad Asghar1, Fiaz Ahmad1 and Muhammad Rizwan3* 

...and stability in various regions or can be used as a resistant source in further breeding programs 

...
Lamiaa Elsayed Mokhtar Deef
...68 bp were identified as regions of the mitochondrial cytochrome b (Cyt b) and mitochondrial 12S rRNA genes, sequences of both genes were placed in the GenBank publication database (Accession numbers are KF783143 and KJ193305) and (Accession numbers are M95109, KF783175, AC175224 and AC160876) of Cyt b and 12S rRNA respectively. Furthermore, DNA barcoding is a widely used molecular-based identification system which aids in identifying species. 
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Shoaib Nawaz1, Muhammad Razaq1, Zahid Mahmood Sarwar1*, Muhammad Sajjad1*, Syeda Aneeza Ubaid1, Muhammad Asif Zulfiqar2 and Usman Haider

...tropical and subtropical regions and cause heavy losses. Neonicotinoid insecticide, like nitenpyram was used on calendar basis and ETL basis. Data was recorded on weekly basis. In first week jassid population in control and ETL treatments was equal with non-significant difference between them while in calendar treatment population was significantly low. ETL and calendar base treatments give maximum yield because numbers of pest in both treated plots remain con...
Tariq Mahmood1,*, Ijaz Ahmad1, Faraz Akrim1, Abdul Hamid1, Muhammad Waseem1, Abid Hussain1 and Muhammad Sajid Nadeem2
...f rough, sloping and dry regions rising to the higher foothill valleys of the internal Himalayan ranges and western Himalayas and plains, Balochistan, and dry hillsides of the Punjab and Sindh. It is the “National Bird” of Pakistan, however, very little is known about its ecology and breeding in its native range in the north of the country. The present study investigated breeding ecology of the bird in its native range of Lower Dir district of Khyb...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
...ethal levels. Within the regions and sexes no significant variations were found in metal concentrations; however within three bat species metal concentrations varied significantly. This study provides baseline data for future comparisons and management of bats and metal concentrations in environment.
...
Gangchun Xu1,2, Fukuan Du2, Yuyu Wang2, Yan Li2, Zhijuan Nie2 and Pao Xu1,2,*
...nd 3′ untranslated regions were 269 bp and 139 bp, respectively. The full length ortholog PTGES2b was 1,457 bp and contained a 729-bp ORF that encoded a protein of 242 amino acids. The 5′ and 3′ untranslated regions were 402 bp and 326 bp, respectively. One polyadenylation signal (AATAAA) was present 14 nucleotides upstream of the poly(A) tail in both the PTGES2aand thePTGES2bgene. The ...

Mudassar Javed, Muhammad Zeeshan Majeed*, Muhammad Luqman and Muhammad Afzal 

...he farmers in Afro-Asian regions. This two-year field study was carried out to evaluate an IPM (bio-intensive) module developed by selecting through in-situ evaluation and incorporating the most effective pest control options along with the biological (parasitoid Trichogramma chilonis egg cards) and cultural techniques against okra lepidopterous borers. According to results, maximum shoot and fruit infestations (i.e. 19.86 and 15.63%, respectively) by okra bor...

Sabi-ur-rehman1, Farooq Azam1, Shaheed Ur Rehman2, Tasbeeh Ur Rahman3, Ayeza Mehmood4, Afshan Gohar5 and Abdul Samad5* 

...tropical and subtropical regions of the world, throughout the America, tropical Africa, and West Indies. The main objective of this review was to highlight the relevant documented knowledge published about its botanical aspects, phytochemistry, traditional uses as well as therapeutic potential of Conocarpus erectus. This plant was selected due to its great medicinal importance like its leaves and fruits have been using traditionally as antipyretic, anti diabet...
Ajmal Khan Kassi1*, Humayun Javed1 and Tariq Mukhtar2 
...on in arid and semi-arid regions.
...
Huseyin Cetin1,*, Oner Kocak2, Emre Oz1, Samed Koc1, Yesim Polat1 and Kalender Arıkan2
...loped resistance in many regions/countries of the world. The use of synergistic substances to overcome insecticide resistance is widespread. Piperonyl butoxide (PBO) is used as a synergist in combination with SPs for the control of houseflies. The aim of this research was to determine the effect of SPs (cypermethrin, permethrin and deltamethrin) and their combination with different rates of PBO (1: 0.125, 1:0.25, 1:0.5 and 1:1) against four Turkish house fly p...

Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3* 

... all sugarcane producing regions of the world. Its incidence could effectively be minimized by planting smut resistant cultivars. For evaluating a large number of propagative materials; standardized germplasm screening protocols as well as optimized inoculation techniques are required. The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wou...
Kamran Zakaria1*, Noreen Munawar1 and Shafqat Ejaz2
...h purpose, Precipitation regions were recognized in river Indus basin in Pakistan by examining the rainfall frequencies with the help of global wavelet power spectra. Records from 52 rain gauges were studied and the outcomes of the whole power spectra exhibited a low annual frequency all over the basin; whereas additional frequencies are present with major significance that indicate changes in the rainfall system. While, the calculated global wavelet power spe...
Madeeha Khalid and Amtul Jamil Sami*
...ly whose members contain regions of very similar conserved sequences making this gene a challenging candidate to analyze. Almost 90% of the sequence of growth hormone gene family members is similar and prone to single nucleotide variations. The sequence variation occurring in the non-coding region of the GH-1 gene leads to altered transcriptional regulation causing various developmental defects in humans. In this report, we have analyzed a short sequence of ~2...

 Tazeen Jamil, Saba Ijaz, Rabia Arif*, Faiza Akram and Muhammad Saleem

...heir insertion in coding regions leads to altered gene expression. We examined the diversity of retroelements in four consecutive generations of different strains of Sordaria fimicola. The parental strains werecollected from two contrasting environments of Evolution Canyon i.e. harsh South facing slope and neutral North facing slope. Retroelements profiles were generated in order to detect retrotransposons. Strains from South facing slope exhibit...
Zhaochao Deng1, Jingchen Chen1, Na Song2, Yongzhen Li3 and Zhiqiang Han1,*
...e center of Indo-Pacific regions.
...
Ashfaque Ahmed Nahiyoon1,*, Shahina Fayyaz2 and Nasira Kazi2
... in the cotton producing regions of five districts of Sindh viz., Sanghar, Mirpurkhas, Umerkot, Mityari and Tando Allahyar. The analysis of samples resulted in the identification of one new species of soil nematode viz., Acrobeloides gossypii n. sp., along with three new record species viz., Tylenchorhynchus ewingi Hopper, 1959; T. crassicaudatus Williams, 1960 and Pratylenchus pseudofallax Café-Filho &am...
Tianlong Cai1, Falk Huettmann2, Kisup Lee3 and Yumin Guo1,*
...n, we suggest that seven regions located at the Korean Peninsula are also suitable for dispersing hooded cranes from Izumi, Japan. Artificial feeding, including setting up feeding stations with grain, artificial cranes (decoys) and artificial flowing water impoundments can be used to attract wintering hooded cranes at these seven sites. Theoretically, our models show that these habitats can accommodate approximately 3,713 wintering individuals (ca. 35% of the ...

Kubra Siddique*, Ghaffar Ali, Irfan Ullah, Atta Ullah Shah and Muhammad Fayaz 

...both the rural and urban regions are price inelastic confirms that they are having important role in the balanced diet and sustenance of Pakistani household therefore this study recommends promoting of chicken and fish production in both the rural and urban regions of Pakistan.  

...
Hayat Zada1, Ahmad-Ur-Rahman Saljoqi1,*, Ijaz Ali2, Bashir Ahmad1, Abdul Waheed Khan2 and Shabeer Ahmad2
... Matta, Madyan and Kalam regions. Out of 30 tested primers, 21 amplified 157 polymorphic bands in three populations. The mean gene frequency (f), genetic distance (I) and Shannon’s information index (h) for three populations were 1.336, 0.304 and 0.437, respectively. Nei’s unbiased measures of genetic identity and genetic distance revealed that higher genetic distance was observed among the isolates from Kalam and Madyan (97.87 %) whereas low genet...

Essossinam Ali* 

...farm households in three regions (Central, Kara and Savannah) in subsistence agriculture in Northern Togo. Tobit and linear regression models were used to analyze the effect of farmers’ risk aversion on fertilizer and DTS uses, and labour allocation, respectively. The results indicate that on average 86.22% of farmers have risk aversion behaviors. Widening the land devoted to maize cultivation would increase farmers’ risk aversion while increasing ...
Zhengfei Wang*, Dan Tang, Xuejia Shi, Huayun Guo, Xiuping Chen, Daizheng Zhang and Boping Tang*
...the important functional regions (protein binding region and helical transmembrane region) in the mitochondrial protein structure.These results help explain why Bythograeidae crabs are capable of living in the hydrothermal vents and suggest that these crabs might have acquired an enhanced capacity for energy metabolism in an extreme hypoxic environment. These findings highlight the critical role of PCGs in the evolution of extreme environmental tolerance by By...
Zhimin Yuan1, Yan Yu2, Yanmei Wang1, Yanzhen Bu1,* and Hongxing Niu1,*
...6S rRNA gene V1−V2 regions were sequenced using MiSeq high-throughput sequencing platform to study the bacterial community of both the stomach and the intestine of H. alaschanicus. A total of 21, 336 and 34, 188 high quality reads of microbiota were obtained from the stomach and intestinal tract of H. alaschanicus, respectively. The phylogenetic analyses showed that the gastrointestinal bacteria were mainly classified into five phyla, domin...
Wen-Feng Li1, Rong-Yue Zhang1, Chun-Hua Pu2, Jiong Yin1, Zhi-Ming Luo1, Xiao-Yan Wang1, Xiao-Yan Cang1, Hong-Li Shan1 and Ying-Kun Huang1,*
...rol in sugarcane growing regions in Yunnan. In general, these natural enemies can be divided into two groups: parasites and predators. The dominant species include Apanteles flavipes (Cameron), Sturmiopsis inferens Townsend and Trichogramma sp., which parasitize the sugarcane borer; Synonycha grandis (Thunberg), Lemnia biplagiata (Swartz), Cheilomenes sexmaculata (Fabricius) and Thiallela sp., which prey on C...
Shujjah Haider1,*, Ayesha Maqbool1, Tariq Pervez1, Saima Parveen1, Arfan Ahmad2, Zahid Iqbal3, Javed Iqbal3, Shahid Mehmood3, Amanullah Khan4 and Sajid Umar5
...er flocks from different regions of district Chakwal, Pakistan. A total of 358 blood samples were collected from different regions of district Chakwal and subjected to serological tests including enzyme linked immusorbant assay (ELISA) and rapid plate agglutination assay (RPA). The overall seroprevalence of MG detected through iELISA and RPA was 29.88 % and 20.67% respectively. Moreover, age-wise study revealed high prevalen...
Kun Wang1,2, Yinglin Cui2, Xu Zhao2 and Changjiang Hu1*
...hippocampus and cortical regions of ICH rats. Furthermore, this treatment significantly increased protein expression of claudin-5, ZO-1 and VE-cadherin while protein expression of DNMT3b and MMP-9 were significantly inhibited. Finally, microRNA-29b level were also lower than the Model group. Our data suggests that XN has significant neuroprotective effects on the ICH model rats, which might help to protect the blood brain barrier by regulating of a cascade inc...

Muhammad Ashfaq* and Inayatullah Jan 

...ided into three climatic regions; the northern climatic region, the central climatic region, and the southern climatic region. The study uses Autoregressive Distributed Lag (ARDL) model for maize crop in KP. This study reveals that the impacts of climatic variables are increasing from the southern climatic region to the northern climatic region. The study also reveals that any negative shock to the crops productivity can be overcome by increasing the area unde...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1Muhammad Faheem Malik3 and Muhammad Idrees4

 

...plifications of selected regions of helicase (186 bps, 2769-2954 nt) and capsid (280 bps, 5461-5741 nt) domain in the bile fluids of two birds. The histological data demonstrated pathological changes in liver and spleen tissues of layer chickens positive for viral RNA. The extensive phylogenetic analysis on the basis of partial helicase domain (ORF1) and capsid protein (ORF2) revealed clustering of Pakistani aHEV (Pak aHEV) strains with members of Orthohepe...
Yue Ren1, Peng-Fei Liu2, Wan-Long Zhu1,*,Hao Zhang1 and Jin-Hong Cai1
...t differences among five regions, food intake, RMR, NST and NPY expressions were higher in higher altitude (Xianggelila, XGLL and Deqin, DQ) than that of lower altitude (Ailaoshan, ALS, Jianchuan, JC and Lijiang, LJ). In winter, body mass and serum leptin levels were lower, and food intake, RMR, NST, NPY and AgRP expressions were higher in XGLL and DQ. All of the results suggested that E. miletus can successfully overcome the physiological challenges of...
Zelle Huma1*, Ahmad-Ur-Rahman Saljoqi1 and Farman Ali2
...collected from different regions of Khyber Pakhtunkhwa for nematodes collection G. mellonella larvae were left in sampling jar and were collected back after 2-3 days’ post application. Dead larvae were then put on the white trap and EPNs has then collected afterward. It is concluded on the basis of results that EPNs is positively recorded in grassy land, tomato field, forest land and poplar trees land in six selected districts of Chitral, Kohat, H...

Khaled Kaboudi* 

... H9N2 is endemic in many regions of the world, such as Middle East and North Africa, where the first outbreaks were described since the end of 1990s, many features about epidemiology and genetic characteristics are still little known. This review summarized the epidemiological situation of H9N2 subtype in poultry in Algeria, Egypt, Libya, Morocco, Sudan and Tunisia. Human infection was reported in Egypt and serosurveys programs showed high positivity, particul...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...lar analysis of specific regions overlapping VP1-VP2 and VP2-VP3 genes of CAV strain from outbreaks in broiler flock (n=45) were undertaken in the Punjab Province of Pakistan. The phylogenetic analysis clustered study strains of CAV in group II similar to those reported previously from China. These findings are helpful for an understanding of currently circulating strains of CAV; however, they require further analysis to intervene in necessary strategies to av...

Waseem Abbas1, Shakeel-ur-Rehman2, Abdul Rashid2, Muhammad Kamran3, Muhammad Atiq2 and Muhammad Ehetisham ul Haq3* 

...tropical and subtropical regions. The present research was carried out to evaluate the comparative efficacy of different against the cotton leaf curl virus vector (Bemisia tabaci) and indirectly the disease incidence. Leaves extracts of four plant extracts i.e. Calotropis gigantea, Zingiber officinale, Allium cepa and Azadirachta indica were evaluated at 3 % concentration against eggs hatchability and adult emergence of the whitefly in lab condition. Two conse...
Awais Bin Shahid1, Moolchand Malhi1,*, Saeed Ahmed Soomro1, Muhammad Giasuddin Shah2, Nazeer Hussain Kalhoro3, Asmatullah Kaka4, Rajoo Mal1, Muhammad Awais Soomro1Saba Parveen Samo1 and Muhammad Nawaz Sanjrani5
... surface area of these 3 regions in SY group compared to C. The epithelial thickness in rumen papillae wall increased (P < 0.05) which was associated with higher (P < 0.05) number of cell layers in stratum germinativum in SY group compared to C. The glutathione peroxidase (GSH-Px) activity increased (P < 0.05) in ruminal epithelium of SY goats compared to C. The present study demonstrated an improvemen...
Nurlybay Kazhgaliyev1*, Talgat Kulmagambetov2, Dulat Ibrayev1, Saule Bostanova1 and Zhanat Titanov1
...ola and North-Kazakhstan regions, the thermoneutrality or comfort temperature zone for Hereford and Aberdeen Angus breeds was within the range of +19-2 +27-31ºC. Analysis of ethology of animals showed that all groups had 0.19 functional activity index; as for the other indices, animals of group I and II groups (Hereford heifers) had a comparative advantage, they were more active and spent more time in moving and feed eating. Taken together, different bree...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
...sms (SNPs) in the coding regions (CDS) of the MyD88 gene have also been reported to be associated with inter-subject differences in responses to Salmonella Pullorum infection in chickens. However, whether the 3’-untranslated region (3’-UTR) of the MyD88 gene is associated with resistance to Salmonella Pullorum infection still remains unknown. In this study, a total of eight SNPs, including three novel mutations [SNP4 (A4...
Muneer Abbas1*, Muhammad Ramzan1, Khalid Hussain1, Abdul Ghaffar1, Niaz Hussain1, Muhammad Irshad1, Mudassar Khaliq1 and Sohail Abbas2
...y of the produce in Thal regions of Punjab. Therefore, experiment was conducted to check the efficacy and feasibility of five IPM tools viz. Pheromone traps, Light traps, Trichogramma chilonis, Bacillus thuringiensis, T-shaped bird perches and Handpicking. Results showed the cumulative effects of all IPM components having 4.81, 4.96, 6.22 % foliage, pod and grain damage, 0.71 larva/plant with 32.28 % yield increase over control treatment. But Mar...
Jun Yan Bai*, Hong Deng Fan, Shuai Yang, Xue Yan Fu, Heng Cao, Xiao Hong Wu, You Zhi Pang and Kun Peng Shi
...of quail, SNP in control regions of cytogenin gene (MyoG) 5’ in China yellow quail, Beijing white quail, Korean quail detected by PCR-SSCP method in this study. Moreover, correlations of control regions of MyoG 5’ with egg quality of quail were analyzed. Results demonstrated that: In egg quail group, six genotypes were detected in Locus A in the control region of MyoG 5’, including AA, BB, CC, AB, AC and BC...
Meriem Msaad Guerfali1,*, Heitham Hamden1, Salma Fadhl1, Wafa Djobbi1, Lotfi Sillini1, Wafa Marzouki1 and Mohammed Ammar2
...ere conducted in several regions to learn about the nature and number of treatments. The samples were subjected to ingestion toxicity bioassays of malathion and spinosad at different concentrations. Histopathological effects of spinosad were also studied and showed that for doses up to LC50, annoying effects begin to appear in glial and neurosecretory cells. The LC
Jiantong Feng, Xueping Wen, Yahong Guo, Yingying Ye*, Jiji Li and Baoying Guo
...t species in the coastal regions of China. In order to characterize the genetic diversity and population structure of M. lamarckii, a 750 bp region of mitochondrial COIII gene and an 822 bp region of 12S rRNA gene were sequenced and analyzed for 118 and 105 individuals from five populations (ZS, WZ, ZP, ST and ZJ) in the Southeast coastal areas of China, respectively. Results revealed that 33 haplotypes were defined in COIII gene, and 30 haplotypes were...
Yunfang Tian, Zeqin Fu, Jiantong Feng, Yahong Guo, Yingying Ye*, Jiji Li and Baoying Guo
...e species in the coastal regions of China. However, the previous studies of genetic information about M. meretrix were vague. None integrated research to reveal the population genetic and structure of M. meretrix in China from north to south. In this study, we investigated the genetic variation of seven location samples distributing the China coastline from north to south by mitochondrial DNA (COI and Cytb genes). The genetic divers...
Aftab Shaukat1,*, Sana Hanif2, Irfan Shaukat3, Muhammad Ahsan Naeem4,5, Shadab Shaukat6, Rizwan Shukat8, Shahid Ali Rajput9, Imran Shaukat10, Mubashar Hassan4, Khalid Mehmood7, Saqib Umar11 and Muhammad Ali Jamil1
...ich can target different regions in PDCD1gene. Multiple sequence alignments were also performed to investigate similarities among mature sequences of these miRNAs. Our data will provide concrete bases for the validation of thesemiRNAs.
...

Rehman Ali Keerio1, Nighat Seema Soomro1, Aijaz Ahmed Soomro1, Mohammad Aquil Siddiqui2, Muhammad Tahir Khan2, Ghulam Shah Nizamani2*, Muhammad Nawaz Kandhro1, Maryum Siddiqui4, Hajra Khan3 and Farheen Deeba Soomro

...d quality, especially in regions where the soils are Zn deficient. Such approaches could contribute to optimizing current fertilization strategies and can help in improving sunflower production. 

...

Nadia Mangrio1*, Muhammad Nawaz Kandhro1, Aijaz Ahmed Soomro1, Nihaluddin Mari3 and Zia-ul-Hassan Shah2

...onutrients in production regions of the sugarcane in Pakistan. This study focused on hypothesis that application of both Zn and B play a key role in getting better sugarcane yield and superiority. Experiment was conducted under field condition during 2016-17 and repeated in 2017-18 at Sugarcane Research Institute, Tandojam, Sindh, Pakistan. Crop in 1st year was planted on an experimental field by shifting on another adjacent field in 2nd year, which was fallow...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1  
...yt-b complete coding regions of both genes were amplified by PCR followed by sequencing. The sequences were aligned and edited using Bio-Edit software and single nucleotide polymorphisms (SNPs) were identified. The boot strapped Neighbor joining tree constructed from MEGA7 explained the genetic relationships of A.chukar with related members of Phasianidae family. The phylogenetic analysis also showed the genetic positioning of A. chukar with ...
Masroor Ellahi Babar*
...of different polymorphic regions includes cysteine nucleotide deletion, AT bases insertion and transvertions (C>G; G>T). Deletion of A bases at c.1-1075 and hetrozygosity (CT) at the c1-1046 were analyzed along with some other SNPs are very important. These evidences points towards the change of expression of those protein which are nearest to the region of gene regulation and enhancer region. After observing taxas several distinct clads of camel breeds ...

Muhammad Safdar Hussain1*, Muhammad Farrakh Nawaz2, Muhammad Ayyoub Tanvir2 and Noor-E-Hira

...la (recommended for arid regions) under water stress. Therefore, a pot experiment was carried out with three treatments: Well-watered, 25% and 50 % drought to achieve said objective. It was found that drought negatively affected plant growth. The mean heights of E. camaldulensis and T. aphylla were maximum (38.16 and 34.7 cm, respectively) at control irrigation while minimum height was found (17.1 and 24.9 cm, respectively) at 50% drought. So, the reduction in...

Gulnaz Hameed1, Abdul Saboor1, Khuram Nawaz Sadozai2*, Ghaffar Ali2, Dawood Jan2 and Mansoor Rasheed

...ticularly in the distant regions. Local communities may be involved and private sector should also be invited by foundation of mutual social conscientiousness to improve education related infrastructure. 

...

Ghulam Akbar1*, Muhammad Anjum Zia1, Ali Ahmad2, Neha Arooj1 and Shahneela Nusrat

...ry to truncate antigenic regions present in the SK gene for the removal of streptokinase antigenicity. The basic goal of this research work is to highlight antigenic regions founded in streptokinase molecule and to give strategy to remove these antigenic sites. 

...
Şükrü Önalan1*, Muhammed Arabacı1and Haşmet Çağırgan2
... obtained from different regions in Turkey were evaluated phenotypically in the study. In all isolates, cream colored, bright, round and S-type colonies with smooth margins were seen in TSA medium. They were alive during native examination without movement. It was observed that morphologically all isolates were Gram (+), α-hemolytic (BA), oxidase and catalase negative and were reproduced under 21, 37 and 45 °C temperatures with 0–6.5 % NaCl sal...
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
... from various geographic regions (southern and northern Xinjiang) based on morphological features, biological characteristics, and gene expression, thus providing a theoretical basis for the genetic manipulation of A. cinerarius. Illumina RNA-Seq was used to perform massive sequencing of 18 cDNA samples derived from male adults, female adults, and pupa of A. cinerarius in southern and northern Xinjiang. We extracted total RNA from 18 samples and ...

Ahmad Kiani1*, Shapour Zarifian1, Hosein Kouhestani1 and Hosein Karimzadeh

...ll the gap between urban regions and rural regions and many immigrants from rural regions to urban regions have been recorded. So, this research tries to prioritize the criteria of establishment and development of agricultural industries in the rural areas in Tehran Province. The result shows the items which refer to market and financial issue have great...

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...quences of the antigenic regions of the outer capsid proteins VP8* were compared with vaccine and field strains. Here we show that the VP4 gene of the P[14] strains detected in this study exhibited close identity with zoonotic Moroccan goat and bovine strains. The amino acid sequences of the antigenic regions inside VP8* proteins showed high conservation between the amino acid sequences of human and animal strains bearing P[...

Muhammad Rafiq1, Muhammad Tariq Mahmood2*, Mushtaq Ahmad2, Imtiaz Ali3, Muhammad Saleem1, Irfan Rasool1 and Zeshan Ali

...tivated in drought-prone regions and marginal lands where moisture stress is a major limiting factor for chickpea production. In order to identify drought resilient chickpea genotypes, a field experiment consisting of 16 elite genotypes was carried out in two environments i.e. under moisture stress and non-stress environment following randomized complete block design at Gram Breeding Research Station, Kallurkot, Punjab, Pakistan during rabi season of 2018-19. ...

Hafiz Muhammad Imran Javed* and Mushtaq Ahmed 

...in. All of the conserved regions were same regardless of the gene while the substitutions were seen at different residues other than the conserved domains. However, the analysis based upon the three-dimensional protein structures revealed that the study isolates showed mutation at C-terminus region of N protein. Such a study helps to improve our understanding towards evolution of emerging and re-emerging strains of SRMVs in the country. 

...

Abdul Raqeeb1,3, Syed Moazzam Nizami2,4*, Aamir Saleem3, Lubna Ansari3, Saeed Gulzar3, Basit Ali3, Masooma Saleem5
 

...ra tree in dry temperate regions of Himalaya has been developed from the empirical data collected from four different locations with three sites each as representative of its naturally growing area. Destructive sampling including excavation of roots was carried for overall 60 trees. The results revealed generic equations for above ground biomass and volume. However, statistical analysis pointed out several significant differences in biomass allocation among C....

Sahib Khan1, Muhammad Naeem2, Tawab Khan3, Nasir Ahmad4* 

4LSB BASED DATA HIDING IN COMPLEX REGION OF DIGITAL IMAGES AND ITS EFFECTS ON EDGES AND HISTOGRAM
...ta hiding in the complex regions of cover image make the use of edges for hiding and communicating secret
information. The complex region in a digital image is the least sensitive to human visual system (HVS) in term of
changes than smooth region. The data hiding in the complex regions affect the edges and the histogram of the image.
These two may cause the existence of hidden information to be detected....

 M. Ihsan Ul Haq1, Ahmad Hussain2*, Farzana Yasmeen3, Shafiq R. Qureshi4

LIFE CYCLE COSTING AND PAYBACK PERIOD EVALUATION OF A SOLAR THERMAL DESALINATION SYSTEM
...iseases in the different regions of the world
especially remote rural and coastal areas. About 97% of water on earth is comprised of seawater. Desalination of
saline water is a prominent approach to handle the problem of water scarcity. Conventional desalination technologies
cause economic and environmental problems due to their dependency upon fossil fuels. Solar flash desalination
is one of the best desalination techniques in the ...

Sajjad Ali1, Muhammad Shahzad Khattak1, Daulat Khan1, Mohammed Sharif2, Hamad Khan1, Asmat Ullah3,
Abdul Malik1 

PREDICTING FUTURE TEMPERATURE AND PRECIPITATION OVER PAKISTAN IN THE 21ST CENTURY
...ood of droughts in these regions. For the end century, positive precipitation departures were projected
throughout the country, except Balochistan, Gilgit Baltistan and Southern Punjab, which showed negative departures
of (-1 to -19%). With the projected rise in mean annual temperature through Pakistan, several sectors such
as agriculture, energy, water supply and health will face serious challenges in the second half of the 21st century.<...

Waqas Anwar, Iftikhar Hussain 

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...elopment between restive regions of Pakistan like Federally Administered Tribal Areas, Provincially Administrative Tribal Areas (FATA/ PATA) / hinterland of Baluchistan and remaining part of Pakistan. Government realized the importance of this under development in the aftermath of 9/11 and consequent unrest and trouble. Construction projects unleashed efforts to bring restive regions in line with rest of country. Project Man...

Waqas Anwar, Iftikhar Hussain 

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...elopment between restive regions of Pakistan like Federally Administered Tribal Areas, Provincially Administrative Tribal Areas (FATA/ PATA) / hinterland of Baluchistan and remaining part of Pakistan. Government realized the importance of this under development in the aftermath of 9/11 and consequent unrest and trouble. Construction projects unleashed efforts to bring restive regions in line with rest of country. Project Man...

 Faisal Shabbir*, Muhammad Fiaz Tahir*, Naeem Ejaz*, Daulat Khan**, Naveed Ahmad*, Jawad Hussain*

EFFECT OF COCONUT FIBER AND MARBLE WASTE ON CONCRETE STRENGTH
...ly available in tropical regions. These two raw materials can be used as a partial cement replacement
in concrete. In this research, a combination of CF and marble dust (MD) has been investigated. Cubes were formed
for compressive strength and beams were formed for flexure strength tests using different percentages of cement, CF
and MD. It has been found that the combination affects the strength of concrete. It was observed ...

Waqas Anwar1*, Iftikhar Hussain1

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...elopment between restive regions of Pakistan like Federally Administered

Tribal Areas, Provincially Administrative Tribal Areas (FATA/ PATA) / hinterland of Baluchistan and remaining part
of Pakistan. Government realized the importance of this under development in the aftermath of 9/11 and consequent
unrest and trouble. Construction projects unleashed efforts to bring restive regions in line...
Sitara Nawaz1, Shamaila Irum1, Haroon Ahmed2, Shazia Shamas1, Sadia Roshan1, Muhammad Ather Rafi4, Irfan Ullah5*, Gulnaz Parveen3*
...of sandflies in selected regions of Panjab and their relationship with hospital-reported cases of Leishmaniasis. Clinical information of 124 patients was recorded. Observable factors of prevalence include age group, gender, financial status, clinical symptoms, and travel history. The sandflies also collected and identified. Out of 350 sandflies two genera Phlebotomus and Sergentomyia were reported, P. segenti was most abundant specie and a...

 Sikandar Bilal Khattak1*, and Iftikhar Hussain1

ASSESSMENT OF ENVIRONMENT CONSCIENCE CONSTRUCTION PRACTICES IN PAKISTAN USING SIGNAL TO NOISE RATIO
...ion differs in different regions. Identification of these factor is the first step towards achieving it. However, no all-inclusive study exists for a developing and terror hit country like Pakistan. This paper identifies and quantifies the environmental conscience factors, respectively. The data is then analyzed through Taguchi Signal to Noise Ratio (SNR) to rank the factors. The results indicate that factors such as type of soil, which has...

 Muhammad Waseem*, Irshad Ahmad**, Muhammad Asif Khan**

DETERMINISTIC SEISMIC HAZARD ANALYSIS AND SELECTION OF TIME HISTORIES FOR DYNAMIC ANALYSIS FOR A SITE IN SWABI
...most tectonically active regions of the world. The latest destructive 2005 Kashmir earthquake of magnitude 7.6 struck the northern part of Pakistan which caused death toll of about a hundred thousand people and monitory losses of about five billion US dollars. After this earthquake, most of research activities were directed to define earthquake hazard. However, amongst other problems in those studies, one hard decision is the use of attenua...
Muhammad Rizwan1*, Bilal Atta1, Ana Maria Marino de Remes Lenicov2, Roxana Mariani2, Arshed Makhdoom Sabir1, Muhammad Tahir3, Misbah Rizwan4, Muhammad Sabar1, Ch. Muhammad Rafique1, Muhammad Afzal5
...e Oriental and Paleartic regions. Laodelphax striatellus was recorded on rice for the first time in Pakistan. Among alternate hosts, Trifolium alexandrium, Leptochloa chinensis, Helianrhus allus, Medicago polymorpha and Sorghum bicolor were recorded for L. striatellus, while Leptochloa chinensis, Helianrhus allus, Medicago polymorpha, Sorghum bicolor, Zea mays and Cynodon da...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...custs found in different regions of the world. In early 2020, in Pakistan, huge swarm of desert locust infestation was observed in different provinces of Pakistan. Precise and correct identification of any pest is very important to start a proper control strategy against it. The current study was conducted to identify and characterize locust species and to determine their association with other swarm forming locust species worldwide. Morphological features and...

Tao He1,2,3, Cheng-jing Chen4, Jian-guang Qin3, Yun Li1,2, Rong-hua Wu1,2 and Tian-xiang Gao4,*

... Wen Zhou and Guang Zhou regions and test a complementary method to quantify the variation of sagittal shapes to discriminate L. haematocheilus stocks. The sagitta variation of five L. haematocheilus stocks was examined using nine shape indices (Roundness, Circularity, Form-factor, Rectangularity, Ellipticity, Radius ratio, Feret ratio, Aspect ratio and Surface density). Multiple comparisons on shape indices showed that there was no signif...

 Honghai Zhang1,*, Yao Chen1, Xiaoyang Wu1, Shuai Shang2, Jun Chen2, Jiakuo Yan1, Qinguo Wei1, Xibao Wang1, Yongqiang Lu3 and Huanxin Zhang2

...gene V3-V4 hypervariable regions. The differences of gut microbiota in the Tibetan wild ass at different altitudes were compared. We obtained 1474595 16S rRNA gene sequences. The study observed 163genera belonging to 19 phyla in Group A while 210 genera belonging to 19 phyla in Group B withBacteroidetes and Firmicutes predominating. Additionally, Bacteroidetes and Firmicutes linearly decreased (P < 0.05) in Group A and linearly increased (P &l...
Zumama Khalid1, Muhammad Abrar Yousaf2, Abeedha Tu-Allah Khan1Farah Rauf Shakoori2, Muhammad Munir3 and Abdul Rauf Shakoori1*
...s prevalent in different regions of the world about the rate of deaths and survivals. Therefore, the current comprehensive review has been written in view of all these aspects so as to present a complete picture of where the world stands, how can the disease be coped, what myths have been developed along with this new pandemic, and what measures should be taken both at individual and states’ levels to help fight against COVID-19.
...

Adnan Arshad1*, Huma Qamar2, Ristina Siti-Sundari3, Zhang Yue1, Muhammad Zubair2, Muhammad Ali Raza4, Muhammad Habib-ur-Rehman5 and Lizhen Zhang1*

Phenotypic Plasticity of Spineless Safflower (Carthamus tinctorius L.) Cultivars in Response to Exogenous Application of Salicylic Acid under Rainfed Climate Conditions
...of production in rainfed regions worldwide.

...

Md. Aminul Islam1*, A.N.M. Aminoor Rahman2, Mohammad Shah Alam3 and Md. Taimur Islam4

Gastrointestinal Nematodiasis in Quail in Bangladesh
...quail farms from various regions of Bangladesh. The fecal samples analyzed by direct smear methods and nematodes identified by the presence of characteristic eggs in the feces. Five types of nematode eggs found on different farms. The overall prevalence of gastrointestinal nematodiasis were (17.5%), which could consider relatively higher. The highest rate of infection found for Heterakis gallinarum (7%) and the lowest rate of infection found for Trichuris spp....

Mohammad Javad Golmohammadi1, Hamid Reza Mohammaddoust Chamanabad1*, Bijan Yaghoubi2 and Mostafa Oveisi3

GIS Applications in Surveying and Mapping of Rice Weeds in Guilan Province, Iran
... important species in 10 regions of Guilan province. The Langarud and Rudsar regions each with 47 species and Anzali and Shaft with 25 species (with 71.2 and 37.9 percent, respectively) had the highest and lowest species diversities, species diversity in 2015 was more than 2014 and 2016 years. The frequency of weed species in Guilan rice farms in 2014-2016 was 3.38, 3.87 and 4.82percent, respectively. Cyperus esculentus and ...
Yawar Iqbal Erum*, Hussain Sagir, Kazi Nasira and Fayyaz Shahina
Compendium of the Genus Psilenchus de Man, 1921 (Tylenchida: Psilenchidae)
...e anterior and posterior regions taken either from the original descriptions or subsequent re-descriptions is also incorporated herein. A total of seven species have so far been reported from Pakistan.

...
Gulzhan Aubakirova1*, Zhanat Adilbekov2, Assylkhan Inirbayev2, Assel Zhamanova2 and Aibar Akhmetov3
...he water bodies of three regions, and the most infection was observed in the fish of the DSU-58 reservoir in the Nurinsky district, where the infestation rate was 42.8%, intensity 3-5. Most likely this is due to the presence of fish-eating birds (herons and tree frogs) and a large number of gastropods in these reservoirs. We detected isolated cases of fish infection with diplostomiasis in roach from Lake Toxumac, where IR was 12.5%. This is due to the presence...
Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
...cted from four different regions of Khyber Pakhtunkhwa province and processed for isolation of campylobacter species. A total of 182 (14%) Campylobacter jejuni were isolated using enrichment and plate media followed by confirmation through multiplex PCR. Isolates were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% bei...

Saeed Ahmad1, Abdul Ghaffar1, Muhammad Habib ur Rahman1,3*, Tanveer-ul-Haq2, Mahmood Alam Khan4 and Arshad Mahmood5

Evaluation of Different Production Systems in Combination with Foliar Sulphur Application for Sunflower (Helianthus annuus L.) under Arid Climatic Conditions of Pakistan
...tems especially in south regions of Pakistan having high arid climatic conditions. A study was conducted in South Punjab (30°1575 N, 71°5249 E) to evaluate the performance of sunflower production systems (nursery transplanted and direct seeded) and sulphur foliar spray (0, 50, 100 and 150 ppm) on sunflower in a randomized complete block, with split plots arrangement. Results revealed that tallest plant (175 cm) with maximum number of leaves (32.7) reco...
Muhammad Ilyas1,2*, Imran Khan2*, Muhammad Umer Chattha2, Muhammad Umair Hassan2, Muhammad Zain3, Wajid Farhad4, Sana Ullah1, Ateeq Shah5, Safeer Ahmed6, Bismillah Khan1 and Muhammad Adeel1
Evaluating the Effect of Zinc Application Methods on Growth and Yield of Wheat Cultivars
...eat crop under semi-arid regions.

...
Hong Hu1, 2, Li Qian1, Yuanlang Wang1, Chaodong Wu1, Xiaodong Zhang1, Yueyun Ding 1*, Li Wang1, Xudong Wu1, Wei Zhang1, Dengtao Li1, Jian Ding1, Min Yang1 and Zongjun Yin1*
... intergenic and intronic regions. There were 2601 upregulated genes, but 284 downregulated genes in Yorkshire pigs compared with those in Anqingliubai pigs. The top 10 most significant Gene Ontology (GO) terms included catalytic activity, binding, cell, cytoplasm, positive regulation of multicellular organismal process, biological regulation, cellular process, etc. There were 54 significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including cyto...
Atef  Mohamed El-Sagheer
Status of Phytonematodes in a Main Commercial Banana Production of Upper Egypt
...viz., Abo-Teg and Assiut regions but present in Manfalot and East Mangabad with the prominence value 1.82 and 0.22 and population densities 43 and 11, respectively.

...

Mohammad Mohsin Faqeer1, Muhammad Aquil Siddiqui2*, Nighat Seema Soomro1, Shameem Raja3, Muhammad Tahir Khan2, Ghulam Shah Nizamani2, Fareen Deeba Soomro4 and Muhammad Mahran Aslam2

Impact of Row Spacing on the Growth and Yield Parameters of Lentil (Lens culinaris L) under Semi-arid Region of Pakistan
...me crop in the semi-arid regions of Pakistan. The current two years study was conducted to determine the impact of row spacing on the growth and yield parameters of lentil varieties. Three lentil genotypes (M-85, NIA Masoor 2005 and NIA Masoor 2016) were evaluated at three spacing treatments (15, 30 and 45 cm) under the RCBD. The morphological characteristics of lentil genotypes depicted that maximum germination, plant height, number of branches plant-1, numbe...

Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
... cells or the regulatory regions that control these genes have been isolated. Transproteins toxic to different plant parasitic nematodes can be expressed into tissues and cells feed upon by nematodes. Transgenic products with a potential to interefere with nematode physiology such as digestive enzymes or structural proteins of the intestine are considered.

...

Ghulam Murtaza1*, Muhammad Ramzan2, Amna Razzaq4, Muhammad Numan3, Rukhsar Beanish4, Ayesha Zafar4, Muhammad Shan Latif3 and Muhammad Adnan3

Screening of Brinjal (Solanum melongena) Varieties against Jassid, Amrasca bigutulla bigutulla
...tropical and subtropical regions of the world. Both nymphs and adults of Amrasca bigutulla bigutulla suck the cell sap from underside the leaves resulting in leaves curling and yellowing. Jassid (Amrasca bigutulla bigutulla) inject toxic materials into leaves which results necrosis. An experimental study was conducted by using randomized complete block design (RCBD) during 2018 to check the resistant brinjal varieties (Nirala, Dilnasheen and Bemisal) against j...

Muhammad Jahanzaib1*, Nazakat Nawaz1, Muhammad Arshad1, Shehzadi Saima4, Muhammad Suhaib2, Muzammil Husain3, Haris Khurshid1 and Shahid Ali Khan1

Effect of Temporal Application of Gypsum on Mineral Uptake and Economically Important Morphometric Traits in Groundnut (Arachis hypogea L) under Rain-fed Conditions
... crop grown in semi-arid regions of Pakistan. Groundnut is a good source of protein, edible oil, and vitamins. A gradual decline in groundnut yield has been reportedly subjected to various agro-climatic conditions and soil fertility problems. In this study, various regimes of gypsum application and its effect on groundnut yield, morphometric parameters, and minerals (Ca, K, P) concentration in root and shoot have been examined. A newly released Pothowar ground...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...-6) and its promoter regions have extensively been studied because of their potential role in the pathogenesis of rheumatoid arthritis (RA). Due to the confounding results, reported in several studies, we performed the systematic literature search on IL-6 -572 G/C polymorphism (rs1800796 SNP) and assessed its association with RA in the overall world population, and in the subgroup analysis of Asian and Caucasian populations. The results showed that in over...
Muhammad Shoaib1, Imran Rashid1*, Haroon Akbar1, Ali Ahmad Sheikh2, Shahid Hussain Farooqi3, Muhammad Asif3, Mumtaz Ali Khan4, Shakeel Mahmood5 and Farhan Anwar Khan6
... and adult animals. Body regions wise distribution of tick infestation revealed that, external genitalia was the most favorite site for tick infestation (73.73%) followed by inner thighs (65%), dewlap (62.21%), neck and back (56.68%), tail (34.56%), around eyes (16.13%), legs (13.36%), ears (11.06%), and flank region (5.76%). This study concludes that Rhipicephalus is the most prevalent tick genus of the study districts. Furthermore, crossbred cattle, y...
Jun Yan Bai*, Kun Peng Shi, Xiao Ning Lu, Xiao Hong Wu, Xue Yan Fu, Heng Cao, Hong Deng Fan, Meng Ke Chen and Yong Kang Ma
...of quail, SNP in control regions of cytogenin gene (MyoG) 5’ in French giant quail, and Savimit quailwas detected by PCR-SSCP method in this study. Moreover, correlations of control regions of MyoG 5’ with slaughter performance of quail were analyzed. Results demonstrated that: In meat quail, three genotypes (AA, BB and AB) were detected at locus A in the control region of MyoG 5’. For ...
Eslam Moradi-Asl1*, Behnam Mohammadi Ghalehbin2, Kamran Akbarzadeh3, Jafar Mohammad-Shahi4, Hajar Afshin4
... were occurs in tropical regions and rural areas where animal contact is common. Wound myiasis occurs when fly larvae infect open wounds. We describe an old male with wound myiasis by agent of Lucilia cuprina, who had a history of diabetes and also blind that reefed to hospital in 13 September and was admitted to date 18 September 2019.Larvea extracted manually from thumb finger of left foot and sent to Medical Entomology Laboratory of the School of Pub...

Bina Khanzada1, Arfan Ahmed Gilal1*, Bhai Khan Solangi1 and Imtiaz Ahmed Nizamani2

Effect of Morphological Characters of Indigenous Sugarcane Varieties on Population of Chilo infuscatellus (Snellen) and its parasitoid, Cotesia flavipes (Cameron)
...widely used in different regions of the world for its management. Considering the significant role of morphological characters of sugarcane varieties on infestation of C. infuscatellus, thus, on parasitism of C. flavipes, this study was conducted in 2013 and 2014. Ten sugarcane varieties i.e., NIA-98, NIA-2004, Thatta-10, Gulab-95, BL-4, L-116, SPS-26, AEC86-223, AEC82-1026 and Larkana-201 were used in the study, each sown on half acre area at Experimental fie...
Ashara Sajid1, Muhammad Usman Ghazanfar1, Saeed Rauf 2, Zahoor Hussain3, Salman Ahmad1 and Yasir Iftikhar1,*
...n";">~703bp) regions. The present study not only detects the molecular detection of CGD in already identified samples through the iodo-starch test but also provides reliability for quick indexing of disease in the aforesaid field. Moreover, molecular detection also revealed the (standardization) of primers used in previous and current studies. Characteristic symptoms combined with molecular detection would be useful to formulate the management stra...
Imran Haider1, Muhammad Aown Sammar Raza1*, Rashid Iqbal1, Salman Ahmad1,2, Muhammad Usman Aslam1, Muhammad Israr3, Umair Riaz4, Maliha Sarfraz5, Nasir Abbas6, Salman Haidar Abbasi7, Zafar Abbas8 and Muhammad Aamer9
 
 
...lly in arid to semi-arid regions. Overcome this, various management practices are used one of them is the use of a growth enhancer. In the present study drought ameliorating effects of amino acid and yeast on wheat (Triticum aestivum L.), development and yield were observed. A field experiment was performed at Agronomic Research Area, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur in November 2019. Factorial arrangement in ...
Guofang Wu1,2, Xingxing Xue1, Wenjuan Shen1, Lei Wang1,2*, Yuhong Ma1 and Jiping Zhou1
...re located in intergenic regions on SSC14 and SSC5, respectively. TGA repeats within the L4-(TGA)n locus were located in the inner mitochondrial membrane peptidase subunit 2 gene (IMMPL2) on SSC18. Further association analysis revealed that the L1-(GT)n locus was significantly associated with teat number (p < 0.05) and that the 152-bp allele exhibited the most positive association. Our data indicate that the L1-(GT)n...
Muhammad Bilal Anwar1, Mirza Azhar Beg1, Amjad Rashid Kayani1, Muhammad Sajid Nadeem1*, Syed Israr Shah1, Sajida Noureen2
Muhammad Mushtaq1 and Tariq Mahmood3
...oup of birds in northern regions. In this study, we compared seasonal differences in the diet of three owl species in an uncultivated area with rapid urbanization all around to better understand their ecological significance. Regurgitated pellets of three owl species (n = 434) were collected seasonally from the study area. The food of the spotted owlet and eurasian eagle owl was found significantly different (P< 0.05) across seasons whereas no...
Iftikhar Hussain1, Sardar Azhar Mehmood1, Muzafar Shah2*, Mohammad Salim3, Shabir Ahmed1, Sana Ullah1,Sidra Batool4, Nadia Saeed1 and Khalid Usman1

 Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*

...over lapping intron exon regions of HR gene followed by DNA sequencing. Analysis of the DNA sequence revealed a novel deletion c.429delC in exon 2 of HR gene. Due to this deletion Proline at 144 changed into Lysine causing frame shift leading to premature termiation of the protein after 23 amino acid residues (p.P144LfsX23), resulting in a truncated HR protein with 166 amino acid residues. The mutation followed Mendalian pattern of inheritance as...

Joseph Anejo-Okopi1*, Jennifer Ifeoma Okpara1, James Mogoret Dabol1, Joshua Adetunji1, David Adeniyi2, David O. Amanyi3, Otobo I. Ujah4, Veronica David1, Patience Omaiye5 and Onyemocho Audu6

...is B patients in endemic regions and other high-risk populations.

...
Tanay Dineshkumar Shah
 

...s are grown in different regions of India. However, the per capita consumption of mushroom in India is very less as compared to other countries though mushroom has many health benefits as compared to other regular diets a normal Indian consumes. Gujarat is one of the states of India where the consumption of mushrooms is less. The paper concentrates on finding out the probable reasons for the people of Gujarat who are not consuming mushrooms regularly in their ...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...onic and three in exonic regions, were identified in STAT5A gene. One exonic polymorphism G→A, in exon 5, was significant that causes a non-synonymous amino acid change from Serine (S) to Asparagine (N). Further, 64 genetic variants were also identified in STAT5A gene and these variants were due to cattle and buffalo differences. Phylogenetic analysis and evolutionary divergence were also estimated. The sequence was submitted to GenBank (NCB...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
... type D in different regions. Similarly, significantly (P˂0.001) higher prevalence was observed in lambs (54%) as compared to sheep (23%). For gross and histopathological lesions a total of 50 animals were necropsied infected with C. perfringens type D. A total of 43 (86%) sheep were recorded having hemorrhages on serosa of intestine followed by gas filled small intestine in 41 sheep (82%), lesion...
Imran Tarique1*, Muhammad Ghiasuddin Shah1, Illahi Bux Kalhoro1
Zaheer Ahmed Nizamani2, Mansoor Tariq 2, Jamil Ahmed Gandahi1
Saqib Ali Fazlani3 and Benazir Sahito3
...n skin of different body regions (dewlap, abdomen, scrotum / udder and thigh) of young and adult of either sex of red Sindhi cattle (Bos indicus). Skin of red Sindhi cattle showed epidermis thickness rang of 41.1-50.13 µm and dermis thickness rang of 443.97-597.05 µm in all body regions and significant (0.05) in age and sex group. The epidermis possessed stra...

Muhammad Ashraf Sumrah1*, Muhamad Jan1, Azhar Hussain2, Shoaib Akhtar1, Hussain Nawaz1, Muhammad Afzal3 and Humara Umar1

Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
...k samples from different regions of goat populated areas of district Faisalabad-Pakistan. Using surf field mastitis test, collected milk samples were screened for subclinical mastitis at the spot for subsequent identification of pathogenic strains of S. aureus through microbiological examination in the laboratory. Non-probability statistical tools conferred 42% (84/200, CI=35.37-48.93) prevalence of subclinical mastitis, 38.1% S. aureus (32/84, C...

Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Abdul Moeez1, Muhammad Zeeshan Manzoor1 and Aneela Riaz3

... growth and yield of dry regions of the world and likewise in Pakistan. Silicon role in plant growth and especially strengthening of plant defence system is well documented particularly upon exposure to salt stress. Present research was performed using wheat variety Punjab-2011 with Randomized Complete Block Design (RCBD) under field conditions using three treatments and each replicated three times. Treatments were wheat florae were developed in plots having n...

Muhammad Rafique1, Inam Ul Haq1*, Humara Umar2, Muhammad Jan2 and Muhammad Azhar Iqbal2

...ion, especially in those regions where wild olive groves exist particularly in Punjab (Pothwar), Khyber Pakhtunkhwa and Balouchistan. Few areas of these regions are considered suitable for its commercial cultivation, where minimum temperature occurs between 10oC and 12oC during end March before olive flowering. In recent past, mass scale olive plantation over an area of 10,000 acres was done in these parts of the country. So...
Amreen Zahra1, Mushtaq A. Saleem1*, Hasnain Javed2, Muhammad Azmat Ullah Khan3, Muhammad Naveed1 and Abdul Rauf Shakoori4
... Pol in the most favored regions. Dolutegravir, a protease inhibitor, was used for molecular docking to reveal the drug resistance sites among the isolates under investigation. However, strikingly the binding energies resulted were -7.9 kcal/mol for Pol and -5.7 kcal/mol in Gag that predicted that SNPs in the Pol gene could be one of the reasons for drug resistance and the primary site of mutations progressing the onset of infection. These computational strate...

Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*

...rms located in different regions of Sindh and to suggest a guideline for favorable physicochemical parameters for fish and shrimp farming. Water samples (n = 3 each) were collected from these farms and analyzed for contents of ammonia, alkalinity, temperature, nitrate, salinity, nitrite, pH, total hardness, dissolved oxygen (DO), chlorophyll-a and phytoplankton-density during the period from March, 2015 to June, 2016. The chlorophyll-a concentration was determ...

Ayaz Ahmed* and Muhammad Zulfiqar

...lakand and Hazara forest regions. The study based on cross sectional data. Data were collected from 321 randomly selected households in three JFM (experimental group) and nearby three non-JFM (control group) villages, located in the same forest-ecological and socio-economic settings. The study analyzed the effects of JFM on local livelihood by using binary logistic regression. Odds ratio analysis of the social and natural assets data showed that the Joint Fore...
Zeynep Sonmez1 and Memis Ozdemir2,*
...s of the identified gene regions of 72 head Holstein cattle, samples were separated by the high-resolution melting analysis (HRMA), and sequence analysis was applied to some randomly selected samples. New polymorphisms were identified in the determined genes and gene regions as a result of the sequence analysis. In the 1st exon of the EPS8 gene, 9 polymorphic regions were...
Muhammad Ramzan1*, Unsar Naeem-Ullah2, Muhammad Umair Sial2, Naeem Iqbal2 and Shafqat Saeed2
...inal purposes in various regions and also known for absorbing various toxic pollutants from the environment. Several insect pests like thrips, whiteflies, mealy bugs and caterpillars attack on these plants. Among them, leaf eating caterpillar, Trilocha varians (Lepidoptera: Bombycidae) is serious insect pest for these precious plant species in various countries. The larvae of the pest can cause 80-100% defoliation. The various confirmed host of T. varians incl...

Muhammad Akhtar1, Muhammad Tariq Mahmood2*, Kaiser Latif Cheema1, Mushtaq Ahmad2, Muhammad Jahanzaib Khalid1, Amir Amin1, Javed Anwar Shah1, Zeeshan Qadeer1 and Zeshan Ali3

...tivars for drought prone regions of the country. For this purpose, a research experiment was conducted in moisture stress (drought) and non-stress conditions (irrigated) at Gram Breeding Research Station, Kallurkot, Pakistan during 2019-20. Mean performance of various traits revealed significant differences among all the included chickpea advance lines. Drought indices i.e. drought tolerance efficiency (DTE), drought susceptibility (DSI) and yield reduction pe...

Ghulam Murtaza1*, Muhammad Ramzan2, Assad Ullah3, Abid Ali5, Ayesha Zafar3, Rukhsar Beanish3, Ahmad Ali4, Ghulam Mustafa4 and Mudassar Aslam4 

...ortant pest in different regions of the world. Due to cosmopolitan in nature, it causes huge losses in various fruits and vegetables production throughout the globe. The current experiment was designed at Insect Rearing Laboratory, MNS. University of Agriculture, Multan to evaluate host and oviposition preference of Bactrocera zonata on different fruits under laboratory conditions. Infested fruits were collected from local fruits and vegetables market and brou...

Aly M. Abdel-Salam

...v>
RT/RNase genome regions of ORF III detected the virus in infected sugarcane leaves
and in its vector Saccharicoccus sacchari and in another unidentified
Pseudococcidae- mealybug. Amplicons for the RT/RNaseH motifs of the two virus
isolates were cloned, sequenced, and submitted to the GenBanak. Pair-wise nucleotide
identity indicated 86% identity between the Giza and Assiut isolates. NCBI-blastn
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1 
...oom and phyllody, in all regions of the screened governorates, reacted
positively when assayed by nested polymerase chain reactions (PCR) using universal
phytoplasma-specific primer pairs P1/P7 and R16F2n/R16R2. Similar assays were used
to detect phytoplasma interactions with experimentally host plant. Dienes’ stain was also
used for detection of natural infection of phytoplasma. The phloem of infected tiss...

Naglaa F.S. Awad1, Gamelat K.F. Kotb2

...of antigenic
regions of VP60 (part of E and F regions) revealed neither detection of recombination points nor sites
under positive selection.
Conclusion: The obtained results indicate that non-hemagglutinating RHDVa G6 variant viruses are
currently circulating in Sharkia with the ability to infect both young and adult rabbits that may be due
to inadequate applicatio...

Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3

...tato
in some regions where vectors easily move into potato fields from reservoir host, particularly if a tuber
necrosis-causing strain is involved.
Objective: : The purpose of this study is to characterize biologically and serologically AMV infecting
potato (Solanum tuberosum L.) in Egypt. Moreover, the study described the histological and cytological
effects of AMV infection in potato leaf cells.
Ruqayya Bint Khalid1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Zubair Shabbir3 and Masroor Ellahi Babar4
... characterize the exonic regions of β-casein gene and to explore the status of A1/A2 β-casein type in Cholistani cattle breed of Pakistan. Blood samples of Cholistani Cattle were collected from Government Livestock Farm, Jugait Peer, Bahawalpur. Genomic DNA was extracted from whole blood by organic method. PCR primers were designed and optimized according to respective melting temperatures. PRALINE tool, MEGA 6.0 and POPGENE tool were utilized for ph...
Zain-Ul-Aabdin Abro1*,Naheed Baloch1, Raza Muhammad Memon2 and Niaz Hussain Khuhro2
...n;1.28,13.4±1.39) regions, respectively. The abundance of fruit flies showed a significant correlation with temperature and slight negative relativity with humidity. The result suggests that high temperature enhances the fruit flies activity in connection with the availability of fruiting parts. The present investigations would be helpful to attain effective integrated pest management campaign in guava orchards.
...

Ahmed M. Soliman

...nd stunting in different regions of Al-Ahsaa at Eastern Province in Saudi Arabia was conducted during the spring of 2016 and 2017. The viral isolates were biologically isolated by single chlorotic local lesion on Chenopodium amaranticolor and propagated in healthy Nicotiana benthamiana. Enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) with specific primers designed for the coat protein gene of CMV were used...

Nusrat Habib

...es living in mountainous regions due to Pakistan’s limited livelihood opportunities. Climate change impact is gendered in the real sense. This study is designed to analyze women’s livelihood situation in mountainous regions in the face of climate change with a qualitative methodology. Focus group discussions, participatory observations, and key informant interviews were used in collecting women’s perspectiv...

Muhammad Rafique1, Muhammad Azhar Iqbal2, Inam Ul Haq1*, Muhammad Ramzan Anser2, Humara Umar2 and Muhammad Ashraf Sumrah2

...n selected agro-climatic regions. The demand for local plants is increasing and no scientific study has been previously carried out to standardize propagation technology concerning optimization of light intensity under greenhouse conditions. The current study was conducted at Izhar farms (Pvt.) Ltd. under the collaboration with Barani Agricultural Research Institute (BARI) Chakwal to find out the appropriate light intensity for successful olive propagation und...
Arshad Khan1, Mohammad Ihsan1, Ali Hazrat1*, Mohammad Nisar1, Muhammad Laiq1, Maryam Bibi1, Nasir Ali2, Ulfat Naz1, Muhammad Zakria1, Nausheen Nazir3, Adam Khan4 and Muhammad Asif Nawaz5
...collected from different regions of district Dir and Swat, Khyber Pakhtunkhwa Pakistan, and were characterized for 11 phenotypic traits (4 qualitative and 7 quantitative). A significant diversity was found for leaf length with the range of 4 to 15 cm, leaf width 1 to 7.0 cm, petiole length range from 0.5 to 4.5cm.The internodes length ranged from 0.5cm to 6. The seed length ranged from 4 to 11mm. The 100 seed weight ranged from 7.7 to 48g. Correlation analysis...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
... distribution in various regions was noticeably different. Differences in dairy farming practices, geographical environments, and the use of antibiotics may be the reasons for the varied distribution of different genotypes in those provinces.
...
Ayman Balla Mustafa1, Abdalla Elgenaidi1, Aldukali Alkeskas1, Asim Faraz2,*, Ahmed Eisa Elhag3, Mohanad Bashari4 and Bernard Faye5
...n desert and semi-desert regions; this milk provides the man with all essential nutrients in addition to its medicinal properties. A study was conducted to determine the impact of parity order on nutrition values of camel milk overall four months postpartum under modern system. Eight she-camels were selected immediately after calving and assigned to two equal groups, early lactation (GY) and mid lactation (GD). Both groups were managed together at the same env...

Muhammad Dawood1,2, Naeem Sarwar2, Muhammad Umer Chattha1, Imran Khan1, Muhammad Bilal Chattha3*, Faiz Hussain4, Muhammad Mahmood Iqbal5, Muhammad Akram4, Hammad Husnain5, Muhammad Shahid5, Mussarrat Hussain6 and Muhammad Umair Hassan1

...od quality for semi-arid regions.

...
Gong Xue-na1, Jia Ting2, Zhang Di2 and Zhu Wanlong1*
...s from Hengduan mountain regions in response to high-fat diet, E. miletus in Jianchuan (JC) and Xianggelila (XGLL) were fed a high-fat (HF) diet for 4 weeks and returned to a low-fat (LF) diet for another 4 weeks, body mass, food intake, resting metabolic rate (RMR), activity behavior, serum leptin levels, hypothalamic neuropeptide expressions and body compositions were measured. The results showed that regions and HF...
Shoaib ur Rehman1, Jabbar Khan2*, Raaza Malja Khan3, Maimoona Azam4 and Zeeshan Mutahir5
...vious results from other regions of the Pashtun ethnic group, which demarcates the heterogeneity in mutations found in the Pashtun ethnicity. These observations may help in arranging counseling about disease recurrence in future, large scale mutation screening and PND not only for Kohat region but also the whole community of KP province. Such study could provide valuable information regarding thalassemia prevention like prenatal diagnosis (PND), genetic counse...

Amina, Muhammad Zahid Rashid and Amina Rashid

...tropical and subtropical regions, but the consumption time is three or four days, so it cannot be sold in a distant market. This trial was carried out to investigate the role of chitosan coating on partially mature guava to retain good quality and prolong shelf life in cold (11°C). Different chitosan concentrations i.e. 20% 30% and 40% were coated and stored at 11°C temperature. These fruits were compared with control (non-treated) fruit after 15 days....

Iffat Nawaz1*, Tahseen Zeb1, Bibi Saima Zeb2 and Javaria Sherani3

...from the three different regions (Himalayan, Mesoamerican and Andean). Furthermore, diversity in qualitative traits (leaf color, leaf pubescence, hypocotyls pigmentation, flower color, pod color, growth habit, dry pod color, pod curvature, pod beak position, pod beak orientation, seed shape, seed coat pattern and seed color) was studied by using standard Chi square test for homogeneity of populations. Results showed that agro climatic conditions have no influe...
Zekeriya Özüdoğru1, Hülya Kara2*, Adem Kara3, Derviş Özdemir3 and Hülya Balkaya2
...denum, jejenum and ileum regions. These distribution patterns also provide further evidence of species-specific differences.
...
Sadaf Ashraf1, Masood Ahmed Siddiqui1*, Kanwal Nisa1, Samar Ali1 and Naeem Rashid2
...ained the four conserved regions that are a characteristic of GH13 family members. Recombinant Pcal_0222 was purified to apparent homogeneity using cation exchange and gel filtration column chromatographies. Purified Pcal_0222 exhibited optimal α-amylase activity at 85°C and pH 5.5. The activity was not dependent on any metal ion. The hyperthermophilic nature and metal ion independence make it a suitable candidate for both basic as well as applied re...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...in different mountainous regions in Pakistan. A proficient way for large-scale and fast propagation of C. tuberculata through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and 2,4-D, alone as well as in combinations. The highest growth response for callusing 78.3% was obse...
Hongbin Li1,2, Lei Fang1, 3 and Lei Cao1,2*
...WI) at different staging regions and on breeding areas. Results showed that, at stopovers south of 52°N, eastern tundra bean geese fed mainly on cropland and arrived at stopover sites ahead of 50% GWI. At all subsequent stopover sites in Russia, eastern tundra bean geese fed exclusively on natural forage land, and followed GWI northwards, finally overtaking GWI to arrive at the breeding sites in advance of these dates. These data show that satellite-derive...

Sanjeela Sabahat1*, Juliya Abbasi2, Mushtaq Ahmad1, Saima Mumtaz1, Taj Naseeb Khan1, Sudheer Tariq1 and Muhammad Imran1

...or a few low-temperature regions, it is mainly grown as an annual fruit crop where runners are transplanted every year. Owing to its short growing period and high profitability, it is mostly produced by small scale farmers. However, the changing climate from the past few years has affected strawberry production in Pakistan. Heavy rainfall, along with hailstorms causes damage at the fruit initiation stage. Moreover, the rainfall also lead to the leaching down o...

Muneeb M. Musthafa1,* and Fauziah Abdullah1,2,3

...si. Lower elevational ecoregions of Malaysian tropical mountains are good laboratories to assess beetle diversity which can be utilized for climate change studies and ecological disturbances. Long term monitoring program between different mountains on beetle diversity and distribution would enhance the understanding of their habitat pattern in relation to seasonal pattern and anthropogenic disturbances.

...

Kubilay Ucar1, Görkem Oruk2 and Sait Engindeniz1*

... Leading plum production regions are the Mediterranean Region, the Aegean region, the Marmara Region and the Central Anatolia Region. In the last 10 years, plum production increased by 113.34% and reached 11,373 tons in Izmir. It has been determined that plum production and area will increase in the next five years. Within the scope of the research, cost and profitability of plum production for 2018 were calculated in Izmir province. The data of this paper is ...

Riaz Shah1*, Shaikha Hilal Al Ismaili2, Sheikha S. Al-Siaby1, Amal Mohammed Al Nasiri2, Thuraiya Hafidh Al Maskari2, Jamal AlSabahi3 and Huda Al-Ruqaishi3

...for basil. The different regions seem to have different yield/composition.

...

Omer Eyuboglu

... habitation of secondary regions pose a critical threat that adversely affects the marine environment and marine based economic activities and applications. The transportation of NIS causes a decline in fish industries, spoilage of culture fishing stocks, rising production costs, adverse effects on human health and changes in biological diversity. The most prominent alien species in Turkish Black Sea waters are  species invasions which are witnessed in th...

Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain

...s. Moreover, immunogenic regions within HA gene may contain potential immunogenic epitopes. The specificity of HA protein produced from E. coli was confirmed through an antibody-antigen reaction on the nitrocellulose membrane. The appearance of 67 KDa protein on the nitrocellulose membrane confirmed its specificity. The intraperitoneal immunization of mice with HA protein along with enhancer (ferund adjuvants) was done and produced antibodies in serum was dete...

Abid Hussain1*, Saifullah Khan2, Shamas Liaqat3 and Shafiullah4 

...it-Baltistan and Chitral regions of Pakistan. The regions are unique in topographical and agricultural perspective from other areas of the country. Cultivated area is just 0.57% of the geographical area. Operational per capita land holding in the region is just 0.05 ha (less than 500 m2) which is insufficient to produce sufficient amount of food to feed a person throughout the year. The arable land in the region is 1.37% of ...
Muhammad Tariq Khan1, Muhammad Ather Rafi2, Riffat Sultana3*, Anjum Munir1 and Sajjad Ahmad4

Jabbar Khan1, Mehwish Jehan1, Zeeshan Mutahir2, Muhammad Rafi1, Muhammad Ismail1, Aamer Abbas1 and Jabbar Tanveer3

...ess workers in different regions of under-developed countries. The present study was conducted to determine the effect of vitamin E and selenium (Se) on physiological and hormonal status of Damani goat in Dera Ismail Khan, under high ambient temperature. Forty Damani healthy, non-pregnant goats having similar initial body weight were selected. The diets of goats in the treated groups were supplemented with Se (0.3 mg/Kg) and vitamin E (50 mg/Kg) for 4 weeks. T...

Nahla Hamada Magd Khalil1*, Ihab Mahmoud Helal2, El-Desoky Hassan Ibrahim Dorrah1, Soad Ahmed Soliman Ismail3 

...y collected from various regions at Damietta governorate, Egypt. The samples were analyzed for detection of organochlorine pesticide residues (OCP) by gas chromatography. The results revealed presence of different types of OCP residues (Dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethane (DDD), chlorodiphenyldichloroethylene (DDE), Alderin, Dieldrin, Heptachlor, Heptachlor epoxide, Alpha hexachlorocyclohexanes (αHCH), Gamma hexachlorocy...

Waqar Khan1, Muhammad Atif Khan1, Waseem Abbas1, Muhammad Shakeel1, Hammad Uddin2 and Bismillah Shah1*

...ens sampled from various regions of Pakistan during 2019–­2021. The study revealed the presence of two species, Exitianus nanus (Distant) and Exitianus indicus (Distant) in the region. Their morphological descriptions, an identification key, and photographs are given. Specimens of all examined materials are deposited at the department of Entomology, The University of Agriculture, Peshawar, KP, Pakistan.

...

Asim Faraz1*, Bernard Faye2, Cem Tirink3, Ayman Balla Mustafa4, Amal AlKharusi5, Morteza Bitaraf Sani6, Nasir Ali Tauqir7, Muhammad Arslan Akbar8, Muhammad Usman Saleem9, Rana Muhammad Bilal7, Abdul Waheed1, Muhammad Shahid Nabeel10

...kistan is at the hotspot regions where the disasters of environmental changes hit severely. The future hope for food security is a camel particularly for drought-stricken areas of the country, such as Cholistan, Thal, in Punjab, and Thar Deserts in Sindh. Camels have the ability to adapt to the harshest climatic conditions when kept for milk, meat, wool, and hides production. Camel production can be enhanced by reducing the age at puberty and calving interval....

Bridget Adah1*, Clara Kwanashie2, Haruna Kazeem2 and Samuel Mailafia1

...inhabitants in different regions in Nigeria.

...

Judith Kiptoo1*, Daniel Mutisya2, Paul Ndegwa1, Ruth Amata3, Lucy Irungu4 and Rotich Godfrey5

...d in most citrus growing regions in Kenya to assess the abundance, distribution and diversity of plant parasitic nematodes from different soil rhizosphere. Nematode population in 200cc of soil and 5g of roots were collected for PPNs extraction by using modified Baermann’s technique and identification by morphological features. The findings indicated that four major genera were abundant in different citrus growing ecological zones in Kenya. The identified...

Eman Alsayed Hammad and Atef Mohamed El-Sagheer

... Pomegranate cultivation regions. By studying the structure of the plant-parasitic nematodes community inhabiting the rhizosphere of Pomegranate fields, it was found that Meloidogyne is the most common genus and harmful one. So, the efficacy comparative of Chitosan, Marjoram emulsion oil, Trichoderma asperellum, and Vermicompost as alternative eco-friendly control agents for root-knot nematode M. incognita infected Pomegranate were evaluated under greenhouse a...

Zilu Zhang1, Ning Wang2, Ce Hou1, Laiba Shafique1, Saif ur Rehman1, Zhuyue Wu2, Zhiqiang Wang1, Mingsong Wei3* and Kuiqing Cui1*

...ults showed that the PIS regions have no effect on polled phenotype of Guanzhong dairy goat, genetic diversity in PIS region of the goat were identified and the key gene polymorphism sites still waiting to be determined.

...

Mujahid Tanvir1, Muhammad Asam Riaz1*, Muhammad Zeeshan Majeed1, Mazhar Iqbal Zafar2, Muhammad Tariq3 and Muhammad Bilal Tayyab1

...tropical and subtropical regions of the world and transmits many zoonotic diseases in humans and in wild and domestic animals. It is primarily controlled by the extensive use of conventional synthetic insecticides against most of which it has developed resistance. This study was aimed at determining the toxicity of selected microbial and synthetic insecticide formulations and botanical extracts against C. quinquefasciatus larvae. Among the n-hexane extracts of...
Mudassar Jehan1, Masroor Ahmed Bajwa2, Mohammad Masood Tariq2, Asim Faraz3*, Abdul Samaad2, Jameel Ahmad1 and Yousaf Hassan Barozai2
...inhabits in the Northern regions in Balochistan. Molecular studies are the base of breed characterization. Hence, nearly 25 unrelated (including both male and female) animals of Balochi breed were sampled for DNA extraction. Some specific markers (15 out of available 30 SSR markers) were employed in the present study to highlight genetic polymorphism. The PCR was utilized for amplification of individual DNA samples. All of the 15 SSR markers were amplified. Af...

Samira Merabet1, Nora Khammes-El Homsi1,*, Lydia Aftisse1 and Stéphane Aulagnier2

...rodents in the temperate regions is adaptively timed with the season under the influence of photoperiod, temperature and food availability. The aim of this study was to investigate the responses of three small-sized murine species to the same physical and ecological conditions in a locality of Great Kabylia (Algeria). Apodemus sylvaticus, and Mus spretus synchronized their reproduction in winter and spring, Lemniscomys barbarus in spring and summer. Photoperio...

Muneeza Zafar1,2,3, Fazli Rabbi Awan2,*, Munazza Raza Mirza3,*, Sumaira Nishat2,4, Sajid Ali Rajput5 and Imran Riaz Malik1,*

...nd 17 SNPs in non-coding regions are proposed as novel most deleterious variants of APOB. We also try to predict the structural model of APOB through protein docking. The results indicate the applicability of in silico approach to propose the most deleterious SNPs of APOB that should be prioritize for future genetic association studies in cohort of cardiovascular patients. While their structural impact on APOB may suggest these predicted nsSNPs possibly be a b...

Muhammad Zeeshan Nadeem1, Muhammad Farrukh Saleem1*, Muhammad Ashfaq Wahid1 and Muhammad Anwar ul Haq2

...equirements in semi-arid regions, particularly during lean period. To improve initial stand establishment and the fodder yield and quality in pearl millet cultivars through seed priming, a two years field study was conducted at research area of department of agronomy, University of Agriculture Faisalabad, Pakistan. Three seed priming treatments viz. hydro priming (48h), ascorbate (40 mg L-1) and calcium chloride (100 mg L-1) including one control (no-priming) ...
Amreen Zahra1*, Mushtaq A. Saleem2, Hasnain Javed3, Muhammad Azmat Ullah Khan4 and Abdul Rauf Shakoori4
...Pakistan including three regions of outbreak i.e. Kot Imrana Sargodha, Faisalabad and Gujrat. Drug resistance mutations such as T12A, I13A, K14R, I15V, K20I, T31S, E35D, M36I, N37D, R41K, K43X, R57K, Q61H, H69K, K70R and L89M were observed across several sites across the whole length of PR region among the isolates. A significant accessory resistance mutation V32I conferring a low level of resistance against non-nucleoside reverse transcriptase inhibitors was ...

Deepesh Kumar Bhuptani1, Atta Hussain Shah1*, Gul Bahar Khaskheli1, Zubair Ahmed Laghari2, Ghulam Shabir Bahram1, Muneer Ahmed Jamali1, Tarique Ahmed Khokhar1 

...rom dairy farms of three regions in Sindh province i.e., southern (Karachi, Thatta, Hyderabad), central (Mirpurkhas, Umerkot, Shaheed Benazirabad) and northern (Naushahro Feroze, Sukkur and Larkana) were collected and analyzed using commercial ELISA kit. The results revealed that AFM1 was statistically higher (P˂0.05) in district Karachi (0.7223 µg/L) as compared to Thatta and Hyderabad (0.5897 µg/L and 0.5697 µg/L, respectively) than FDA li...

Sohail Ahmed Talpur1, Imran Khatri1*, Maqsood Anwar Rustamani1 and Zubair Ahmed2

...e collected from various regions of Sindh, Pakistan, in total three species of the genus are described; their habitus images and male genitalia is provided; Chaetocnema belli Jacoby, 1904, Chaetocnema concinnicollis Baly, 1874 and Chaetocnema pusaensis (Maulik, 1926). Distributioanl map for each species is also provided.

...

Ngoc Tan Nguyen1*, Pham Kim Ngan Nguyen1, Thao Huong Phan1, Tan Loi Le1, Thi Khanh Ly Nguyen1, Tuan Thanh Hoang2, Cong Thieu Pham3, Cong Dinh Nguyen3, Nguyen Khang Duong4 

...populations in different regions of Vietnam based on nucleotide sequence in a displacement loop (D-loop) of mitochondrial DNA (mtDNA). Thirty-one blood samples that were collected from three native buffalo populations such as Bao Yen (BY: 7 samples), LangBiang (LB: 7 samples) and Thanh Chuong (TC: 7 samples) and two imported buffaloes from Thailand (T: 5 samples) and India (Murrah – M: 5 samples) were extracted for total DNA and amplified about 760 bp mt...

Marcos E. Bollido1,2*, Renell Jay G. Villaluz1,2 and Ronald L. Orale1

...were imported from other regions. Locally sourced pig supply is pegged at 53%, and the rest (47%) are imported from various towns in Samar and other regions. The demand for pork and live pigs is 27% and 55% higher than the supply. The said gap can be filled in by local farmers/raisers with an overall market opportunity of 70,996 kilograms monthly.

...

Shakeel Ahmad1, Muhammad Israr2*, Rasheed Ahmed3, Anam Ashraf1, Muhammad Amin4 and Nafees Ahmad5

... Karakoram and Himalayan regions of Pakistan. For the sustainable maintenance of the mountainous ecosystem, comprehensive research is recommended about these LULC vulnerabilities locally and regionally.

...

Muhammad Ikram Ullah

...ut, and three homozygous regions were identified. Whole exome sequencing was carried out to identify the mutation in putative gene/s. The data was arranged, and MAN2B1 was selected for the DNA sequencing. Protein homology was analyzed by the pymol tool to predict the effect on the mutant protein. A novel missense mutation c. 2710A>T; p.904Tyr>Ser was detected, and co-segregation analysis was established in the complex neurological family. The pymol analy...
Zain-ul-Aabdin Abro1*, Naheed Baloch1, Raza Muhammad Memon2, Niaz Hussain Khuhro2 and Iram Shaikh1
...rchards of both climatic regions. Minimum population of both fruit flies were obtained in January from guava and mango orchards of different climatic zones. The fruit flies density were affected by temperature and relative humidity. However, the stronger connection of population increase with matured fruits were exhibited. The present efforts would be supportive to eliminate Bactrocera species from different orchard agro-ecosystem of Sindh by using MAT in inte...

Zain-ul-Aabdin Abro1*, Naheed Baloch1, Raza Muhammad Memon2, Niaz Hussain Khuhro2 and Waseem Akbar Qazi2

...nd upper (Larkana) Sindh regions for population surveillance of parasitoids during 2019. The results shown (P<0.05) maximum number of Trybliographa daci were (55.60±4.52, 46.40±5.47) obtained from guava infested fruits of both regions. Moreover, Maximum number of Bactrocera zonata were emerged from infected guava (381.00±10.85, 259.40±16.95) collected from lower and upper ...
Rafiq Ali Khan1, Sajjad Ahmed1, Salim Raza2* and Shehla Gul1
...t Sub basin and frontier regions of Samana Ranges. The Kohat Sub basin and adjoining areas are contributing more than 50% of to the current oil production of Pakistan.

 

...

Marat Kuibagarov1, Assylbek Zhylkibayev1, Dinara Kamalova1, Anara Ryskeldina1, Nurdina Yerzhanova1, Yerlan Ramankulov1, Alexandr Shevtsov1*, John A Angelos2  

...rom geographically close regions, some of the studied isolates had unique amino acid sequences that were assigned to new M. bovoculi PilA groups designated K (2 isolates), L (2 isolates), and M (1 isolate).

Keywords | Moraxella bovoculi, infectious bovine keratoconjunctivitis, pilin, pilA, PilA 

...

Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana  

... the exon 9 and intron 9 regions, which were located at the 18 A>G, 213 T>C, +49 A>T, +232 A>C, and +252 G>A. There were 3 transition type mutations (at positions 8, 213 and +252) and 2 transversion type mutations (at positions +49 and +232). Based on the result of this study, it can be stated that the genotype frequency of Pesisir cattle population was not in the Hardy-Weinberg equilibrium.

Keywords | SNP, FSHR gene, Pesisir cattle, DN...

Lobna M.A. Salem, Nashwa O. Khalifa, Marwa O. Abd El-Halim* 

...es and 79 camels} in the regions of Qualyobia, 51 (17.8%) had Fasciola spp. morphologically {27/51(52.9%) were identical to F. hepatica while 18/51(35.3%) were identical to F. gigantica, 3/51(3.9%) were larval stages and 3/51(3.9%) mixed infections}. DNA from 10 flukes extracted, amplified, and analyzed to identify species using the ITS2 locus. Ten flukes were identified as F. gigantica and F. hepatica. PCR products from 2 flukes were sequenced for phylogeneti...

Reza Aghnoum*, Hamid-Reza Sharifi, Masoud Ghodsi and Ahmad Zare Fizabadi

...n widely adopted in many regions of the world; however, the effect of CA practices on non-plant pathogenic nematodes is not well understood. This study was carried out to determine the effect of CA on population density of fungivorous (Aphelenchus avenae) and free-living nematodes under four crop rotation patterns. The experiment was arranged as a split plot in randomized complete block design with three tillage systems (conventional tillage, minimum tillage a...

Ty Nguyen, Giang Thi Huong Phung, Lan Le Thuy Hoang and Giang Van Tran*

...ty of M.cunnesius in two regions of the North and the South of Tam Giang lagoon. Amplified and sequenced 10 samples of CO1 gene of M. cunnesius sample in Tam Giang lagoon were recorded size of 650 bp. The average ratio of four types of nucleotides is A: 23.38%; T: 32.62%; G:18.15%; C: 25.85%. The rate of difference in CO1 sequences of the 2 samples is low compared to previous studies. The similarity coefficient of M. cunnesius in the two areas of Tam Giang lag...

Sherif Abdelghany1, Hossam Mahrous Ebeid2*, Ahmed Abdelkader Aboamer2, Mohamed Ali Radwan1, Rania Agamy1 

Masixole Maswana, Thinawanga Joseph Mugwabana and Thobela Louis Tyasi*

...ristics of the different regions. The current state of information on breed level characterization documented data is not enough and generates a low level of research interest on conservation of donkey genetic resources. The objective of the current review was to describe the morphological variations between different donkey breeds. This review study shows forms of slight variation in morphological traits among donkey breeds of the same area within that countr...

Chanathip Thammakarn1*, Sawanya Umphonphison1, Paitoon Kaewhom2, Kanokrat Srikijkasemwat1 

...mercial farms at various regions of Thailand during the year 2018-2021. A total 2,002 serum samples were collected from 16 farms located in 10 provinces and tested by Enzyme-Linked Immunosorbent Assay. Among total serum samples, 914 samples were further tested by polymerase chain reaction (PCR) to detect ORF2 gene. The results revealed that 81.17% of pigs had low positive level antibody against PCV2, while 10.29% had high positive titer. On the other hand 8.5...

Mushtaq Hussain Soomro1, Jan Muhammad Mari1, Imtiaz Ahmed Nizamani1, Arfan Ahmed Gilal2* and Muhammad Ibrahim Kubar2

...against RPW in different regions of the world, we started experiments in June 2018 to evaluate the synergistic effect of various food baits on the performance of Ferrolure+ against RPW. Three locally available food baits i.e., date palm, sugarcane, and banana along with Ferrolure+ (positive control) and detergent water (negative control) were evaluated against RPW. Three hundred grams of each food bait along with Ferrolure + was used in the traps as weekly obs...
Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3
...b samples from different regions of Turkey (n=350) were collected and studied. The validation of the GlobalFilerTM Amplification Kit was carried out in accordance with the guidelines published by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our study was conducted to show the efficacy and performance of the kit for sensitivity, specificity, limit of detection, dynamic range, limit of quantification, stochastic threshold study, repr...

Monis Hussain Shah1*, Rizwan Rafique2,6, Munawar Almas1, Muhammad Usman3, Sadia Yasin4 and Sajida Bibi5

...ubtropical and temperate regions. Pakistan is one of the top fruits producing country globally. Pakistan ranks 4th in Mango (1.880M Tones), 6th in Guava (2.4 M Tones), Dates (0.72 M Tones) and Citrus (2.1 M Tones) production around the globe. Since the year of 1969 Pakistan builds the infrastructure for agricultural development due to that; significant increase in production were observed during second, third and fourth development plan. Small land holdings, p...

Cristina Stanca Moise1*, Cornelia Chimisliu2, Mihaela Arinton3, Tom Brereton4 and George Moise1

... in Romania’s nine regions, also considering the collecting period. Considering the gaps in studying the distribution of this protected species in Romania, our paper forms a new baseline for future surveys and studies on Lucanus cervus.

...

Asim Zubair1*, Zohaib Atta Mehdie2, Iffat Batool3, Ghulam Akbar Malik4 and Ayesha Aziz5

...ns that characterize the regions of the world. So, if we look at the situation in Pakistan it is affecting rapidly that’s why Production and growth rate of Agriculture is badly affected because of it. Its destruction on Agriculture sector is massive in Pakistan. Changing in climate is directly affecting the livelihoods of Agricultural communities in Pakistan. It has also become the reason of rising temperature and lot of other problems in agriculture sec...
Alia Tajdar1,3, Shaghef Ejaz2, Muhammad Shah Zaib1, Anum Ishfaq1, Syed Muhammad Zaka1*, Hafiz Muhammad Safeer1 and Khalid Abbas1
...tropical and subtropical regions. It damages the fruits at early ripening stage and reduces the quality and the final yield of fruit crop. Moreover, to maintain the quality parameters of fruits after harvesting, is an important aspect of post-harvest management. For the management of fruit fly and to keep the natural quality of fruit after harvesting different methods are used (cultural practices, mechanical practices, biological practices), but chemical contr...

Quang Minh Dinh1*, Tran Thi Huyen Lam2,3, Y Thi Nha Nguyen4, Thanh Minh Nguyen1 and Tien Thi Kieu Nguyen5

Salma Bessalah1, Samira Jebahi2, Asim Faraz3*, Amel Raoufi2, Cem Tırınk4, Walid Dridi2, Mahmoud Daouad2, Hassib Keskes5, Touhami Khorchani1, Khaled Farah2, Abdul Waheed3 and Mohamed Hammadi1

...apped in the crystalline regions were moved toward the boundary regions and the amorphous phases disappeared. XRD patterns showed a new peak at 2θ= 65.8° and a diminution of the relative intensity for the peak of 2θ= 20.54° after 30 kGy. FTIR measurements revealed structural deformations from 5 kGy through chain scission of C–H chains as well as the deformation of the C=O carboxyl groups with increa...

Barbosa Carla1*, Gregori Fabio2, Thomazelli Luciano1, Oliveira Amanda1, Araújo Jansen1, Ometto Tatiana1, Marcatti Roberta3, Nardi Marcelo3, Paludo Danielle4, Utecht Nathalia1 and Edison Durigon1

...n samples from different regions of Brazil were subjected to a RT-Nested-PCR protocol, with primers targeting RdRp region. Positive samples were confirmed using Sanger followed by Ion Torrent S5 sequencing platforms and results were submitted to phylogenetic analysis. From total of 8 confirmed samples, 3 clustered to Deltacoronavirus genus and 5 to Gammacoronavirus. Two of those samples showed partial sequences of other regions<...

R. Singh1† and R. Z. Sayyed2

...ntration in the infected regions as compared with normal healthy sections.
Protein localization found more pronounced at the sites of infection of M. incognita viz., cortical cells, giant cells,
abnormal xylem, medullary region and nematode body. Active RNA synthesis in infected cortical, medullary and
vascular regions was observed by positively dark stained nucleoli, nucleoplasm and cytop...

K. A. Tabassum, F. Shahina†, K. Nasira and Y. I. Erum

... different agro-climatic regions of Sindh, Punjab and Azad Jammu & Kashmir, Pakistan. All species
belong to insectivora group on the basis of leptoderan bursa, crochet needle-shaped spicules, normal rectum, lateral
field with six separate lines and had unique ribosomal DNA-ITS, sequence. A compendium of the genus Oscheius
(of both insectivora and dolichura groups) based on the following characters: body length, tail, spi...

Zarema Alimsultanova Magomedova1*, Kheda Khalitovna Dadaeva1, Roza Said-Akhmedovna Zakhkieva1, Khasan Khamitovich Shakhbiev1, Boris Kazievich Laipanov2 

...ns are widespread in the regions of the North Caucasus (KBR, CHR, KCHR) with a total EI of 44-68%. Associative invasion of intestinal nematodes (Toxocara canis and Ancylostoma caninum) was observed mainly in rural settlements with EI = 36.0%. in urban settlements with EI = 24.0%. In the group of dogs infected with the mixed invasion of Toxocara canis and Ancylostoma caninum, the new tableted Helmicide F at a dose of 70 mg / kg of body weight had EE and IE - 10...

Syed Shah Mohioudin Gillani1, Muhammad Naveed Tahir1, Adeel Anwar1*, Syed Ijaz Ul Haq2, Muhammad Awais3, Mujahid Iqbal4, Javed Iqbal5, Hina Ahmed Malik3, Syed Muhammad Zaigham Abbas Naqvi6, Raza Ullah7 and Muhammad Abdullah Khan1

...tial resolution at wider regions. This will help the scientists to monitor and manage the nutrient requirements of plants as well as will enable them to accurately and rapidly respond to plant health by determining chlorophyll contents in plants.

...

Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5 

...nt of local bull in many regions is currently leading to the development of local bulls and Indonesia is no exception, especially in South Sulawesi Province. One of local bulls in which developed for approximately last ten years is Bali-polled bull. Thus, the selection of polled bulls is crucial, mainly in modern livestock management. The aims of this study to identify sperms post-thawing quality and proteomic plasma semen as fertility performance in Bali-poll...
 
 SADAF SARFRAZ1*, SAFDAR AMEER1, REENA AMBREEN1, SHOMAILA SIKANDAR 2, SHAISTA NAWAZ3 AND YASIR SALEEM
...n collected from various regions of Asia. In this study, antimicrobial activity of cinnamon was estimated using well diffusion method against various bacterial and fungal strains. As shown by the findings of the present study, Cinnamon oil is found a potent antibacterial agent against all microbes used. Additionally, it was found that cinnamon samples collected from Sri Lanka has the highest oil content (3.1%), followed by China (2.8 %) and India (1.9 %), wher...
Jian-Cheng Zhai1,2, Sheng-Nan Wang1,3, Qiang-Hui Wang1, Yan-Ling Xia1, Wei-Shi Liu1, Ya-Jie Yin4 and He-Ping Li1*
...). The 5´ flanking regions of IGF1, KGF and NGF were cloned from reindeer and sika deer antlers, and the lengths were 2089bp/2107bp, 1474bp/1474bp and 865bp/865bp, respectively. Based on bioinformatics analysis, the regions of IGF1 (-50bp ~ +615bp), KGF (-285bp ~ +207bp) and NGF (-388bp ~ +109bp) were selected for studying the methylation status. The antler mesenchyme of male reindeer was the only methylation sample in...

Irene S. Gamil1* and Dalia Fouad1,2

... bp of its 18S rRNA gene regions was carried out to determine the phylogenetic position of the new species among other proteocephalideans. Ophiotaenia tessellata sp. n. shows a close relationship to O. lapata Rambeloson, Ranaivoson and de Chambrier, 2012 parasite of the endemic snake Madagascarophis colubrinus from Madagascar; both infect African colubrid snakes.

...

Usman Ali1,2*, Basharat Ahmad1, Riaz Aziz Minhas1, Muhammad Kabir3, Muhammad Siddique Awan1, Liaqat Ali Khan1 and Muhammad Bashir Khan1

...t Baltistan and Mansehra regions of Pakistan.

...

Oussama Zghari*, Mouloud Lamtai, Sofia Azirar, Mohamed Yassine El-Brouzi, Hajar Benmhammed, Aboubaker El-Hessni, Ali Ouichou, Abdelhalem Mesfioui

...icant, affecting various regions of the brain and causing many neuropathological and neurobehavioral abnormalities as well as oxidative stress (OS). Conversely, melatonin (MEL) has been considered as an antidepressant, anxiolytic substance and protects neurons from OS. The present study was designed to evaluate the neuroprotection effect of MEL against Al neurotoxicity and OS in male Wistar rats. Rats received an intraperitoneal and/or a single intrahippocampa...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...collected from different regions of Northern Punjab, Pakistan. Isolation of pathogen from meat samples were performed with the use of International Organization for standardization based microbiological techniques, while chain reaction technique (PCR) was used for detection and characterization of Stx-1 and Stx-2 genes. In total, 75 (46.8%) isolates were detected as E. coli. Among them, 14 (8.75%) isolates were tested positive for Stx genes (Stx-1 and Stx-2). ...

Asim Zubair1*, Ayesha Aziz2, Ghulam Akbar Malik3, Iffat Batool4 and Zohaib Atta Mehdie5

...h and stability of rural regions as well as to agricultural production. Rural men and women both contribute significantly to the agriculture business, including cottage industries, animal production, and crop production. For this research data was gathered from 100 female respondents using random sampling technique. The data that had been gathered, was examined using SPSS. According to the findings of our research, women in the study area were energetically in...

Budi Guntoro1*, Agung Triatmojo1, Bambang Ariyadi2, Nguyen Hoang Qui1 

...al of 868 farmers from 4 regions in Yogyakarta Province participated in this study through face-to-face interviews using a multi-stage random sample method and quota sampling. Descriptive statistics were used to analyse primary data from the survey. Farmers were chosen by the criteria of who are staying and raising beef cattle in Yogyakarta. The results showed that farmers had a high experience and more than 50 years old, they worked as a farmer at the farm wi...

Fakhra Nazir1*, Sahar Fazal1 and Fakhar-i-Abbas2

...collected from different regions of Pakistan. Mitochondrial DNA was successfully extracted from keel tissue and Folmer region of COI gene comprising ~650 bps was amplified using universal primers and PCR products were confirmed by 1% agarose gel electrophoresis. Sequencing was carried out by Sanger’s method and BLAST analysis identified these samples as five species (Urocissa flavirostris, Dendrocitta vagabunda, Corvus splendens, Corvus corax and Corvus ...

Aqleem Abbas1, Mustansar Mubeen2, Waqar Younus1, Qaiser Shakeel3, Yasir Iftikhar2*, Sonum Bashir2, Muhammad Ahmad Zeshan2 and Azhar Hussain1

...smitted to GB from other regions with food supplies and spillover. In addition, the evolution of new pathogen and pest strains causes catastrophes to GB’s cereal and horticultural Crops. Moreover, Traditional plant varieties that resist disease and pests have been supplanted with fragile but high-yielding cultivars. As a result, diseases like rust, smut, black scurf, early blight, late blight, potato leaf roll virus, potato virus Y, grey mold, mildew, cr...

Ying Liu1,2, Ji-Yong Fang2, Na Zheng3 and Hai-Long Wu1*

...1892, acquired from four regions of Anhui Province, southeastern China. To our knowledge, one species of the Seuratascaris genus, namely Seuratascaris numidica (Seurat, 1917) Sprent 1985, has so far been recorded. The morphology of S. schmackeri sp. nov. differs from that of S. numidica and exhibit a few unique characteristics, including more denticles in the lip, shorter intestinal caecum, longer spicular, more caudal papillae, and pre-, ad-, and post-cloacal...

Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*

...man nutritional needs in regions of the world where they can be properly managed.

...

Muhammad Salman Ikram1, Tahir Mehmood1,2*, Sehrish Firyal3, Huma Sattar4, Shagufta Saeed3, Fareeha Nadeem3 and Muhammad Imran Mahmood Khan5

...s diversity in different regions of world which helps to understand the pattern of variation in DNA within and between species, importance of Y-STR in relation of its haplotype diversity and frequencies among different population, role of paternal lineages identification which helps to determine the mutation rate of Y-STR and its relationship with paternal lineages determination. When familial searching is coupled with genealogy inquiry, the review article wil...

Abdullah*, Imtiaz Khan, Muhammad Ishfaq Khan, Muhammad Ibrahim and Muhammad Fawad

...pea crop in the southern regions of Khyber Pakhtunkhwa, Pakistan, during the Rabi season 2022. Five chickpea growing regions were selected in chickpea growing area of district Karak. The quadrate method was used to determine the relative weed density. The data were recorded on density (%), relative density (%), frequency (%), relative frequency (%) and importance value of weed species. The present findings revealed that Asph...

Dede Kardaya*, Deden Sudrajat, Dewi Wahyuni 

... source feed in tropical regions. Therefore, in this study, we substituted protein sources of the feed with urea which was processed with slow-release technology to avoid urea poisoning. Slow-release urea-impregnated zeolite (UZ) and Indonesia’s natural zeolite (Z) were examined using 24 heads of seven-eight months old of local male lambs (20.12 ± 2.1 kg BW) allotted to four treatments in a randomized group design to determine how it affects lamb ...

Alisher Safarov1*, Nasreen Nasreen4, Firuza Akramova5, Shukhrat Djabbarov1, Adolat Mirzaeva5, Javokhir Esonboev5, Djalaliddin Azimov5, Mourad Ben Said2,3 

...istan and encompassed 12 regions (Andijan, Namangan, Fergana, Samarkand, Jizzakh, Syrdarya, Bukhara, Kashkadarya, Surkhandarya, Navoi, Khorezm, and Tashkent regions). Results: The study identified a total of 23 ectoparasite species belonging to the classes Arachnida and Insecta, encompassing 758 carnivorous mammals. Among Arachnida, three families were represented: Ixodidae (12 species), Sarcoptidae (1 species), and Demodeci...

Kinza Javed1, Muhammad Ali1, Waqas Farooq2, Majeeda Rasheed1*, Farkhanda Yasmin3, Samiya Rehman3, Sadaf ul Hira1 and Jawad Ali3

... is about 3 % in endemic regions of Asia while the morbidity is upto10%. The LSDV is transmitted through the entry of blood from outsource by biting of arthropods including mosquitoes and flies from infected animal to healthy one. To control the dispersal of LSDV, infected animals should be quarantined, restricted its movement and place them in a place of no entry of vectors, arthropods. The recent review investigated new knowledge on several facets of the dis...

Nadia Jabeen1, Abdul Mubeen Lodhi1*, Rehana Naz Syed1, Muhammad Ali Khanzada2 and Alia Gul3

...igned from the conserved regions as described in the previous studies. The amplified PCR products were sequenced from Macrogen Korea, via worldwide scientific Pakistan. Phylogenetic analysis of the obtained sequences was done based on the maximum similarity method using Mega 6.0. The spore was smaller as compared to Marasmiellus scandens (6.2-8.7 µm). The newly described species i.e., Marasmiellus agrianum N. Jabeen and M. Lodhi differs from other specie...

Abeedha Tu-Allah Khan and Abdul Rauf Shakoori*

...itions in distinct brain regions is still lacking. To address the said gap in knowledge, first we have identified the TFs specific to IL-1β a key player in mediating inflammatory process using online databases. Next, we identified the interacting partners of the significantly differentially expressed TFs out of the selected ones, and the mRNA expression changes were studied for both the differentially expressed TF along with its interacting partners. Fina...
Yousef Ahmed Alkhamis1,2*, Basanti Mondal3, Roshmon Thomas Mathew2, Ganesan Nagarajan4,5,6, Sheikh Mustafizur Rahman3, Md. Mostafizur Rahman7, Adnan Alhajji1 and Md. Moshiur Rahman3,8*
...ventually die in coastal regions where salinity changes happen regularly because of different natural calamities. This study was conducted to explore the effects of periodic changes of salinity on different phenotypic traits in Nile tilapia (Oreochromis niloticus) in order to find out any traits that can be expressed through compensatory growth. Equal sized juvenile Nile tilapias were randomly assigned into two treatments including control treatment (CT: const...

Nasir Ali1,2,6, Muhammad Ilyas2,3, Nazia Akbar1,2*, Gohar Rahman2, Shakirullah Khan4, Mujaddad Ur Rehman6, Abdul Samad5, Habib Ahmad1,2 and Bibi Nazia Murtaza7*

...les from three different regions (Chitral, Swat and Hazara) were analyzed for hyper variable sequences of mitochondrial DNA for the determination of their maternal affinities. Our results revealed 25% unique haplotypes in ancient samples whereas 28% unique haplotypes have been observed in the modern samples. The most frequent haplotype found was H2a (23% in ancient and 10% in modern samples). Our results show that with the exception of ancient Dir, there are g...

Jemi Diporianto1,4, Denny Widaya Lukman2*, Yusuf Ridwan3 

...f disease spread between regions. Trichinellosis is one of the diseases transmitted through animal-based food products. Currently, the utilization of wild boar meat has increased for feed and food purposes, making it challenging to control the movement of wild boar meat for trade. Wild boars are more likely to transmit Trichinella spp. than domestic pigs due to the lack of animal health monitoring in wildlife. This study aimed to assess the risk of introducing...

Sumaira Kousar* and Safdar Ali Shirazi

...allenge, particularly in regions susceptible to a confluence of natural and anthropogenic factors. This study endeavors to unravel the intricacies of soil erosion within the Swat district, employing the Revised Universal Soil Loss Equation (RUSLE) model alongside cutting-edge Remote Sensing (RS) and Geographic Information System (GIS) techniques. The RUSLE model, celebrated for its simplicity and efficiency, offers a lens to assess soil losses quantitatively, ...

Ayoola J. Shoyombo1, Ake A. Moses1, Comfort I. Ukim2, Mustapha A. Popoola2, Olayinka O. Alabi1, Noah C. Edozie1, Faith Ogbor1, Jacob Kuusu1, Ekemini M. Okon3* 

...or protein in developing regions. The effect of seasonal variation on reproductive endocrinology was examined in a group of West African Dwarf (WAD) does and bucks. Throughout the study, the animals were kept in the same environment. The results showed that most hormones in the does were nominally superior during the wet season compared to the dry season, while progesterone and luteinizing hormones were significantly (p<0.05) higher during the wet season. O...

Razia Sultana1, Shinawar Waseem Ali1*, Ghulam Murtaza2 and Shahid Mahmood2

...uencing of hypervariable regions (V3 and V4) of the 16S rRNA gene. In the composite yogurt sample, 100% bacteria were detected with a total count of 40423. The number of phyla was 3, of which proteobacteria showed the highest abundance (89.9%). Four classes of bacterial microbiota were detected in which the proportion of class Gamma proteobacteria was the highest (84.8%). The numbers of orders, families, and genera to which bacteria belonged were 9, 10, and 15...

Waseef Ullah Khan*, Yasser Durrani and Muhammad Ayub

...tropical and subtropical regions. Its renowned taste is celebrated worldwide, whether enjoyed fresh or in processed forms. This research was undertaken to assess the efficacy of employing both natural olive leaves extract (OLE) and minimal chemical preservatives (sodium benzoate and potassium sorbate) for preserving mango OLE blend over a 90 days period. The findings demonstrated that the inclusion of OLE effectively preserved the chemical quality and sensory ...

Shafqat Ullah1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Raheela Taj3, Fatima Syed3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Muneeb Islam7 and Sumaira8

.... Samples from different regions (duodenum, jejunum, and ileum) of the small intestine were collected and kept in the fixatives. Slices from each part were stained with H and E stain to make it fit for microscopy. Picture were taken with different magnifications, i.e., 4X, 10X and 40X and histo-morphometry was done through Pixel-Pro (Labomed America Inc). The statistical analysis was through IBM SPSS (Statistics.V22.X86). The present study concluded that selen...

Ikramullah and Inayatullah Jan*

...a and other water-scarce regions

...

Shudong Liu1,2, Shuai Wang1,2, Haoran Cui1 and Genyuan Chen1,2*

...hisms (SNPs) and genomic regions that are associated with ACMS in the Chinese Merino sheep population. To identify the genetic risk factors of alopecia in Chinese Merino sheep population, we carried out a genome-wide association study (GWAS). The 60 Chinese Merino sheep alopecia cases and the 190 Chinese Merino sheep controls were from the same livestock farm. DNA was extracted from ear tissue using the saturated phenol-chloroform method. The DNA was genotyped...

Muhammad Mansoor1, Shahid Hameed Khalil2,4*, Muhammad Arshad Khan3, Ghani Akbar2, Muhammad Shahzad Khan4, Rana Naveed Mustafa2 and Salah ud Din5

...all productivity in arid regions of Pakistan. This study underscores the importance of adopting appropriate irrigation schedules for mung beans in such environments.

...

Nafissa Sahel1*, Fadela Chougrani2, Abderrahim Cheriguene3 and Zineb Hamani1

...collected from different regions of Southwestern Algeria. Samples were collected from 48 camels of El Bayadh, Bechar, Adrar and Tindouf. A wide variation was observed in the physico-chemical properties due to variation in feed, lactation period and environmental conditions. Microbiological results indicate that camel milk from Tindouf was the most contaminated with total aerobic flora, psychrotrophic flora, total and thermos-tolerant coliforms, faecal streptoc...

Adnan Ibrahim Khamis1, Jasim Mohammed Saleh2*, Najwa S. Ali3 and Anwer Thamer Ghaffoori2

...Governorate through nine regions that represent the focus of capacity development. The research was conducted in Anbar province for two reasons: First, because it represents a third of Iraq by area, and the second reason is that it was described as one of the least stable provinces because of the military operations it was subjected to and had a great impact on all aspects of economic, social, and cultural life. The research method is to use the questionnaire ...
Yi Zhao1,2,3, Lei Luo1,2, Liqi Tan4, Jianhua Huang1,2, Dongliang Liu1,2,5, Shigui Jiang1,2 and Lishi Yang1,2*
...neither in the different regions nor in the different season.

...

Khalid Ali Khan1,2,3* and Hamed A. Ghramh1,2,4

...wa, central and northern regions of Punjab provinces but nowadays it is growing rapidly across the country. Three native species of honey bees including Apis dorsata, A. florea, and A. cerana whereas one exotic species A. mellifera are present in Pakistan. Honey produced in Pakistan enjoys good repute in the Middle East due to its unique taste and quality. Pakistan exports around 4,000 tons of honey with a worth of about $ 23.00 million to Arab countries every...

Abdul Aleem Memon1*, Inayatullah Rajpar2, Ghulam Murtaza Jamro2, Javaid Ahmed Shah3

...calcareous soils on many regions. High calcium amount in soil solution suppresses potassium (K) uptake by plants possibly due to K-fixation and variation in cation ratios. Soils in country are thought to be well supplied with K, little or no K fertilizer is applied to majority of the crops, including sunflower. However, recent studies have shown that the sunflower is becoming more responsive and exhibiting superior growth and yield with the addition of K. To d...

 Shawki, Khaled K.; Carter, M.J.; Alnashar, Nariman M., El-Farrashl, Mohamed A. and Taher, Sahar

...ing those immunodominant regions (P2subdomains) that evoke type-specific responses and bridging the resulting gap with a synthetic poly-glycine chain. This construct was expressed using the baculovirus system and the obtained purified protein was examined by EM which showed that the new engineering approach is permitting some form of structural assembly. Our study points a way to produce a more pan-reactive vaccine in future studies by producing antisera direc...

Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni

...y in at least two coding regions.

...

Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.

...okh Tambesha and Salamon regions) and Demiate Governorate and were tested in dot-blot hybridization. BEFV was detected in 24 out of 36 samples (66.7%) as follow: 9/9 (100%) correspond to Berket El-Sabaa and Tokh Tambesha regions and 3/9 (33.3%) correspond to Demiate and Salamon regions. In conclusion, a local Egyptian probe was designed and applied in the dot blot hybridization technique f...

El-Absawy, E.A.; Mahmoud, Amal; Hemeida, A.A. and Helmy, M.

...gene and 3' untranslated regions (UTR). Phylogenetic tree showed two main strain groups: Group I regroups PVYN and PVY stains, while Group 11 includes pvy0, pvyw and PVYN:O strains. The Egyptian PVY isolate was clearly classified within group I, and was more closely related to PVY strains. Ten nucleotide substitutions resulted in 3 conserved amino acid substitutions (VI*I, G7*E, M or V and S8*G) and were able to differentiate between both groups. The partial c...

El-Dougdoug, Kh.A.; Rezk , A.A.; Dawoud, Rehab A. and Sofy, A.R.

...nd USA at sites occur in regions corresponding to the conserved, variable and right terminal domains which are believed to control viroid pathogenicity. Finally, this constitutes the first isolation and identification of CSVd from diseased Chrysanthemum plants in Egypt

...
Ashraf M. Metwally*, Ausama A. Yousif*k , Iman B. Walaa A.   Attia M. Samy * , and Ismail M. Reda *
 
...e in some of the exposed regions of the viral protein. Our findings explain the continued presence of vvIBDV in intensively vaccinated flocks.

...

*Amal Abou El-Ela A., M. A. Amer and Eman A. H. Khatab,

...s derived from conserved regions in the genome of potyviruses was designed to amplify a 335 bp cDNA fragment from infected plant using degenerate oligonucleotide primers specific for detection of Potyvirus group. Amplification of total RNA extracted from infected carnation suggesting the presence of one Potyvirus in the tested carnation plant. Nucleic acid spot hybridization assay also was used to confirm identification of the present virus as one of the Potyv...

A-New-Whitefly-Transmitted-Geminivirus

...at least at the flanking regions of each gene. According to the results obtained from the PCR products and indicator host plants, it can be concluded that they are two different Geminiviruses. Based on host range and symptomatology, the TYMV-QaIubia appeared to cause infections only to some species of the family Solanaceae, in contrast to TYLCV. It was indicated that TYMV could be mechanically transmitted to several species of the family Solanaceae with the ai...

Musaad A. Al-Dubaib

...-Qassim, and surrounding regions were tested for bovine leukemia virus (BLV) antibodies for different animal species. Agar gel immunodiffusion was carried out using commercial diagnostic reagents. All samples of herds having positive cases with immunodiffusion were tested by ELISA. Positive cases were recorded among samples of the Holstein cattle with both serological tests. Prevalence rates differed from one farm to another and with both serological tests. Wi...

Maria Kikelomo Adegun1*, Femi Godwin Ekundayo1, David Daisi Ajayi2 

... the grasses in tropical regions dry up during the prolonged dry season, sheep can survive on urea-treated cassava peels and Gliricidia sepium fodder. 

...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

...GM6 antigen from several regions was carried out. The protein sequence was reverse translated to the nucleotide sequence, after which the gene was synthesized using solid-phase method. The gene fragment, encoding the GM6 protein, was inserted or cloned into the pET28 expression vector after synthesis. The obtained sequences were checked by sequencing for correspondence to the matrix molecule. For transformation E. coli BL21 (DE3) strain was used. To assess the...

Shakir Ullah1* and Lubna Shakir

...in the Arrang and Bajaur regions. while it was present in Ghargu samples at even < 2 months. The phasing out by red fluorescing compounds in stems (SG5) and leaves (SG7) as the plant age increased was attributed to the loss of chlorophyll (green pigments) as a plant part matures. The results indicated that the relationship between phytochemical yield and age of harvesting is not linear. Harvesting at a lower age of < 2 months gives fewer phytochemicals t...

Taleeha Roheen1, Shagufta Kamal1*, Shazia Anwer Bukhari1, Ghulam Mustafa1 and Saima Rehman2

... paddy soil of different regions of Punjab, Pakistan were studied using phylogenetic analysis. Morphological and biochemical analysis showing the presence of different species of novel methanogenic archaea and sMMO-producing methanotrophic bacteria in rice paddy soil samples. The evolutionary analysis indicated a phylogram with five monophyletic groups (MPG-I to MPG-V). Results revealed that three isolated species (Sphingomonas sp. MG-1, Sphingomonas sp. MG-2 ...

Shakir Ullah1*, Lubna Shakir2, Ghani Subhan3 and Mohammad Sohail4

... production in semi-arid regions. Developing new soybean cultivars with high efficiency and ability to drought resistance is very important. The field experiment was conducted to investigate the impact of irrigation regimes at different growth stages (Full irrigation, irrigation regimes at vegetable growth, flowering, and pod full stages) on yield and yield components of two soybean varieties (1448 and Egyptian) under semi-arid regions...
Yongtao Xu1, Dandan Wang1, Xiaolong Hu1, Minling Li1, Ming Tang1, Wuhua Liu2, Jianwen Zhan2 and Weiwei Zhang1*
.... The metabarcoding trnL regions of the chloroplast genome from 30 fecal samples of Taohongling sika deer were amplified with universal primers and sequenced by high-throughput. We found that 28 out of 30 valid samples had a total of 677,856 valid amplified sequences with an average valid sequence of 24,209.14±323.83. A total of 326 OTUs were obtained from 28 valid samples, and the MP, SS, and NJS groups shared 222 OTUs, accounting for 68.09% of the tot...

Muhammad Nadeem1, Muhammad Naveed2,3*, Muhammad Shafiq2, Irfan Rasool2 and Muhammad Afzal Zahid2

...cross different climatic regions of Punjab, and other provinces could contribute in attaining self-sufficiency in local chickpea production as well as plummeting import bill.

...

Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2

... microbiota in three gut regions (foregut: the rumen, reticulum, omasum, abomasum; midgut: duodenum, jejunum, ileum, and hindgut: cecum, colon, rectum) of two riverine type buffaloes using PCR-denaturing gradient gel electrophoresis (DGGE) method to profile the bacterial community and PCR amplification using species-specific primer sets for 16S rDNA fragments to detect major fibrolytic and non-fibrolytic bacteria. Results from the PCR-DGGE analysis showed diff...

Shahid Iqbal1, Arshad Khan, Ali Hazrat1*, Gul Rahim, Mohammad Ihsan1, Umar Zad Gul1, Maryam Bibi1, Khadija Bibi1 and Muhammad Mukhtiar2

...collected from different regions of District Dir Lower. A total of 12 morphological parameters were recorded, of which 3 of them were qualitative characters: leaf colors, seed coat color, and seed shape. For quantitative trait, the maximum coefficient of variance (0.95%) was found in petiole length, biomass per plant (0.49%), and pod per plant (0.41%), whereas the minimum coefficient of variance showed by leaf width (0.25%), followed by leaf length (0.27%) and...

Aniqa Qaisar, Shabana Naz* and Sajida Arooj

...t different geographical regions of Punjab (Jallo Wildlife Park Lahore, Wildlife Park Bahawalnagar and Wildlife Park Murree). For that purpose, Surface soil, deep soil, water, fecal and feed samples were collected as indicators to estimate the heavy metals pollution in the environment of captive P. cristatus. Cr. Pb, Ni, Co and Mn were identified through atomic absorption spectrophotometer. The highest accumulation of heavy metals was found in feed samples fol...

Bela Putra1*, Budi Prasetya2

...g livestock nutrition in regions with prevalent acidic soils. The experiment involved the application of various gamma radiation doses (0, 5, 10, 15, 20, 25, and 30 Gy). Four replications were done using a randomized block design, with 25 Pennisetum purpureum cv. Mot seeds in each repetition, totalling 700 seeds. The plants were grown in acidic soil with a pH of approximately 4.5–5. After two months of growth, the plants were harvested, and various param...

Tri Anggraeni Kusumastuti1, Ikuo Kobayashi2, Ahmad Juwari3, Lovin Dika Antari4*

...airy cattle farms in two regions, namely East Java Province and Miyazaki Prefecture based on the consideration that they are cattle production centers. SWOT analysis is used to support a description of the condition of internal factors (strengths and weaknesses) and external factors (opportunities and threats) of animal husbandry in two countries. Data analysis was carried out descriptively and quantitatively. Primary and secondary data were collected from Jul...

Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2

...buffaloes in subtropical regions of southern Nepal and to characterize the ovarian cyclicity status during the months of long day length. Data regarding seasonality pattern of breeding and calving of Murrah cross bred buffaloes (n=400) from two locations of subtropical region were collected. In a subset of buffaloes (n=104), the ovarian cyclicity status during late spring (May to June) was determined through transrectal ultrasonography and measurement of blood...

Xin Niu1, Xuelan Fang2, Sang Ba3, Yihao Fang1,4, Davide Fornacca1,7, Kun Tan1,4, Yanpeng Li1,4,5*, Zhipang Huang1,4,5* and Wen Xiao1,4,5,6,7

...in high altitude montane regions, the habitats along the overall elevational gradients should be included in wildlife conservation policies.

...

Ahmed Kareem Kadhim Al-Wasmee1, Waddah Salam Hassone2, Hawraa Talib Al-Janabi3, Hamed AH Al-Jabory1

...December 2022 in several regions of Babylon Province. Ninety jugular vein blood samples were collected. The calves ranged from <1 M to ≥4 M and both sexes were represented. Two ml of blood were placed in sterile, EDTA-treated tubes, and were sent to the parasitology lab in an ice pack. The blood was put in a deep freezer set at -20oC for DNA extraction. This genomic DNA purification was used following the instructions provided in the kit to extract the p...

Igor Ponjiger1, Milutin Kovacevic1*, Vladimir Markovic1, Zoran Ristic1 and Vukan Lavadinovic2

...specially in the lowland regions. Even though European hare mostly adapted to intensive agricultural habitats, the population densities have decreased throughout Europe since the 1960s. Previous research reported field size to be one of the most important predictors of the hare population. We aimed to assess the effect of habitat heterogeneity and structure on hare habitat selection i.e. population density. Using hunting organization census data we explored th...

Pakistan Journal of Zoology

April

Pakistan J. Zool., Vol. 56, Iss. 2, pp. 503-1000

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