Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*

...t in milk indicated that proliferation of S. aureus was successfully ceased until six hours post infection when applied at and MOI of 100.

...
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2
...mphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of avian leukosis virus subgroup A (ALV-A) infection was confirmed by necropsy, hist...

Zahir Shah, Ahmed Nawaz Tunio, Shakoor Ahmad, Ijaz Ahmad, Javid Ali, Sher Bhader Khan

...moved with care to avoid proliferation of uterine contents into the peritoneal cavity. Penicillin powder was dusted into the surroundings area of the uterus. The cow was treated with antibiotics, analgesics and liver tonics during the first week post-surgery. Local antiseptic dressing was performed using povidone-iodine daily for one week and skin sutures were removed at 14 day post-operation.

...

Sitansu Pan and Amrita Das

Population proliferation and spread of Trichoderma spp. in soil under two different delivery systems
...t application population proliferation (build-up) and spread of Trichoderma spp., Trichoderma harzianum and Trichoderma viride in three different types (sterilized, solarised and natural) clay loam soil at 50% water holding capacity (pH 6.5) was studied both under laboratory and field conditions following two types of delivery systems, through organic based (vermicompost + neem cake (90% + 10% w/w)) and seed based (sterilized wheat seed) inocula. The populatio...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...ation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
...

Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3* 

...tors (VEGFRs). Survival, proliferation, migration and invasion of endothelial cells which give rise to the vascular network, is mediated through VEGF/VEGFR activation of the signal transduction pathways. Thus blocking VEGF is an efficient strategy for blocking tumor angiogenesis. Several anti-VEGF drugs have been developed over the last two decades and some are still at different development stages of pre-clinical and clinical trials. In this study, we explore...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...nt human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regulated TF...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
... which can regulate cell proliferation, differentiation, apoptosis, and involve in the development of sperm. Meanwhile, DUSP4, PDK4 and FKBP1B were also found to participate in testis development and spermatogenesis. In this study, online software and luciferase reporter gene system were used to predict and verify the target relationship between miR-122 and DUSP4/PDK4, miR-449a and FKBP1B. Thereafter, comparison analys...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

...yric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots per shoot at an average root length of 3.86 cm. After acclimatization and transplantation, 90% of the In- vitro derived plants were found heal...

 Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*

COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
... Additionally, the shoot proliferation response to increasing level of BAP was found genotype dependent. Naphthaleneacetic acid (NAA) also showed a positive effect on in vitro growth responses (shoot proliferation and root induction) when combined with BAP. The media containing NAA, BAP and kinetin accelerated the growth in terms of shoot mass in two accessions, 4494(1) Kowar Sufaid and 4472(12) Harker Agoon and again the cu...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...lpha;1 demonstrated anti-proliferation activity as assessed by MTT assay. This study also showed that N-terminal fusion of SUMO withIFNα2-Tα1 is effective for its soluble expression and to make its purification process more convenient.
...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
...to disease, cell growth, proliferation and lipid metabolism, including PPAR, are important during development of the adipose tissue. These data indicate that future research should emphasize the study of key genes related to synthesis, metabolism, and growth of adipose tissue during development. This will allow to determine the functional relevance of the genes and pathways uncovered.
...
Ayesha Noreen1, Dilara A. Bukhari2 and Abdul Rehman1,*
...pathways, promoting cell proliferation, invasion, inducing angiogenesis, inflammation and cellular transformation in cancer. A better understanding into the mechanism of ROS in cancer progression might be useful for the development of biomarkers and therapeutic strategies. The objective of this review was to summarize the roles of ROS in different stages of cancer, cell invasion, angiogenesis and metastasis.
...
Muhammad Khan1,*, Amara Maryam2, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1 and Yongming Li3,*
...on without enhancing the proliferation of cells and BHS-I was equally toxic to both BaP-treated and untreated A549 cells. Taken together, the data of present study indicate that BHS-I not only inhibits metastasis but also induces cell death in BaP-treated A549 cells. Therefore, BHS-I may become a potential lead compound for treatment of lung adenocarcinoma.
...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...ed microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDSCs were induced into Adipocytes, osteoblasts, chondrocytes and neuron-like cells by the optimized inducing medium. After the two-step digestion and differential attachment technique, high pur...

Mehboob Arslan* and Xin Yang 

.... For absolute survival, proliferation, and propagation inside the host cell, viruses modulate certain metabolic pathways to destabilize the host metabolism. These alterations in host metabolic signaling as a result of virus-host interaction generate a favorable environment for the viral pathogens. This phenomenon is a hotspot for investigations in order to improve the diagnosis and drug development of future antiviral therapeutics. In fact, metabolism is cons...
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
...APs promoted erythrocyte proliferation and increased hemoglobin content. The possible mechanism is by increasing Cyclin D1, Stat5 and Bcl-2 protein expression.
...
Xiaopeng Tang1, Wen-qin Su1 and Re-jun Fang1,2,*
... calcitonin (CT) on cell proliferation, NaPi-IIb expression, and the absorption of phosphorus in porcine intestinal epithelial cells (IPEC-1). The experiment consisted of 1 control treatment and 5 levels of CT treatments (1×10-12 to 1×10-8 mol/L), and each treatment has 6 replications. Cell proliferation was determined by the MTT method. Cell morphology and phosphorus concentration in the ce...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *  
...a improved the microbial proliferation and shortened the time that the substrate reached to the fermentation temperature, resulting in the retention of N, P, K and the reduction of the NH3, H2S from the fermentation litter.
...
Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
...sarcoma U2OS cells. Cell proliferation was measured using CCK-8 assay and colony forming assay. Flow cytometry was used to determine apoptosis and cell cycle profile. Western blot was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase arrest and apoptosis. Further studies showed that piceatannol induces mitochondrial apoptosis as shown by Bcl-2 family proteins modulation. Finally, ...

 Li-na Li1, Sheng Li2, Ping Gui3, Jing-hui Li1, Fen Ai4,* and Li-li Cai1,*

...CCM inhibited MRC-5 cell proliferation and reduced collagen deposition as measured by CCK-8 assay, Sircol assay, qPCR and western blot. MSCCM also reduced the expression of the pro-fibrotic TGF-β1 and increased the expression of anti-fibrotic IFN-γ at both mRNA and protein levels. Furthermore, we found that MSCCM inhibited the activation of NF-κB signaling pathway induced by irradiation as well. Our data suggest that MSCCM could reduce irradia...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...athway of inhibiting the proliferation of MP.
...
Rimsha Munir1, 2*

 

...ased or increased– proliferation rates when cultivated as 3D tumor spheroids. The presented work aimed to study the interplay between cell proliferation rates and metabolic stress in 2D and 3D cell culture systems. Two cell line models (HepG2; liver cancer cell line and HCT-116; colorectal cancer cell line) were selected that showed inverse proliferation trends under 2D and 3D cell c...
Tian-Yi Zhao1, Zi-Qing Liu2, Shu-Fei Ma3, Bo-Yang1, Fan-Fan Guo1 and Ming-Hua Duan1*

 

...ractylolide on PC12 cell proliferation, growth inhibition rate and apoptosis. In order to assess the spatial learning and memory abilities of AD rats, Morris water maze model was applied in vivo, and the activity of the NF-κB signaling pathway and concentrations of TNF-α, IL-6, and IL-1β were measured. The results demonstrated that biatractylolide can reduce apoptosis in hippocampal cells, prevent and improve the cognitive decline induced by A...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*
...termination the index of proliferationdogs adipose-derived stem cells (DADSCs) on IVth and Xth passages. Established that DADSCs contains multipotent stem cells, that are characterized by an almost homogenous fibroblast-like cells оn the IVth and Хth passages. It was difined, that fibroblast-like cells оn the IVth and Хth passages was expressed cytoplasmic and membrane proteins, which are sp...

Mohammad Aslam Khan*, Samiullah* and Zulfiqar Ali*

DYNAMICS OF URBAN SPATIAL GROWTH AND PLANNING: A CASE STUDY OF PESHAWAR
...le to control their proliferation for sustainable development of the city in future.
...
Feng-Mei Yang1, Rui Li2, Xiu-Xue Hu3, Yong Liang4, Bo Gao3* and Wei Chen3*
...) and western blot; cell proliferation inhibition rates (CPIR) of HSF were obtained by MTT, and HSF apoptosis was detected using Flow Cytometry (FCM). UVB irradiations significantly inhibit HSF growth, showing positive correlation with the increasing of CPIRs and apoptotic rates; The relative expression levels of β-catenin and Senescence marker protein-30 (SMP30) gene in UVB treated group were significantly lower than that of the control group; Gene expre...
Aribah Naz1*, Uzma Badar1,Afsheen Arif2*, Zaid Qureshi1, Sondas Saeed1,Erum Shoeb1, Maqsood Ali Ansari1 and Obaid Yusuf Khan1
... stability, survival and proliferation of species in their respective habitat.
...
Yujun Zhao and Jianping Fan*
... without medication, the proliferation inhibition rate of cells processed with Kanglaite injection (10, 20 and 40 μL/mL) significantly increased (p <0.05), the apoptosis rate increased (p <0.05), the expression of Bcl-2 protein decreased (p <0.05), the expression of Bax protein significantly increased (p <0.05), and the activity of Caspase-3 and Caspase-8 increased (p <0.05) in a dose-dependent manner. Hence...
Shikang Deng1,2, Yan Jin2, Jing Xu2, Xiufang Zhu2, Pinghai Hu2, Jiao Li2, Li Zhang2 and Jianzhong Tang2,*
... used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed that after 5-FU intervention, the proliferation of bile duct scar fibroblasts was inhibite...

Inam Ul Haq1*, Humara Umar2, Naeem Akhtar3, Muhammad Azhar Iqbal2 and Muhammad Ijaz1

Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review
...is technique is used for proliferation of olive (Olea europaea L.) tree which is globally cultivated for centuries and its value added products are consumed worldwide. The traditional propagation techniques of olive are uneconomical with wastage of time while micropropagation of olive may expedite the development of important characteristics like biotic and abiotic resistance with high yield and premium quality olive oil. However, the commercial micropropagati...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
... and are also capable of proliferation and differentiation, ensuring the efficiency of passing transgenes from parents to subsequent generations. In this research, to understand the growth characteristics of buffalo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then ...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...on, degradation and cell proliferation.
...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
...The relationship of cell proliferation and apoptosis is important in normal kidney development. The ZFX gene is important for regulation of growth, proliferation and differentiation in tissue cells while the Bcl-2 and BAX genes regulate apoptosis. This experiment adopted the real-time fluorescence quantitative technique to test ZFX, Bcl-2, and BAX expression in mice kidney tissue on 1 d, 7 d, 14 d, 21 d, 42 d, and 90 d after...

Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.

...tocytes leading to their proliferation and triggers HCC. Manganese superoxide dismutase (MnSOD) is a nuclear-encoded antioxidant enzyme that neutralizes free radicals. Val16Ala single nucleotide polymorphism (SNP) is one of the most widely investigated SNPs in the MnSOD gene, where C to T transition resulting in the con-version of valine to alanine at amino acid 16 within the mitochondrial signaling se-quence. This study was designed to investigate the associa...

Shimaa M. Gad1, Ahmed A. Kheder1, Mohamed A. Awad 2

...ing phyllody, yellowing, proliferation, virescence and little leaf symptoms
was observed Giza, Egypt.
Objective: The current work aims the detection and molecular identification of phytoplasma infecting
Gazania in Egypt.
Methods: Phytoplasma disease was detected and isolated from naturally infected gazania plants during
surveys in flower nurseries and open filed in Giza governorate, it was transmitted...

Abdelaziz A. Yassin, Ayman H. El Deeb, Abeer E. Mansour, Hussein A. Hussein

...vaccination. Lymphocytic proliferation assays demonstrated the continuous response of lymphocytes started at the 3rd day till reaches peak at 28th day post vaccination then start to decline. Whereas the peak titers for antibodies were reported at the 28th day post vaccination. Indeed, the study reports the role of ail adjuvant in stimulating the cellular response for inactivated vaccine.

...
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
... AGR2 was tested by cell proliferation and wound healing assays, while the auto-fluorescent property of DsRed was confirmed by fluorescence microscopy and immunofluorescence. The potential application of his-AGR2-DsRed was demonstrated by its internalization in fibroblasts. Therefore, the purified recombinant fluorescent protein would be a very useful tool for further investigation of AGR2’s extracellular function and molecular mechanism.
...
Kanwal Haneef1,2, Rakhshinda Habib1,3, Nadia Naeem1,3 and Asmat Salim1,*
...their ease of isolation, proliferation, anti-apoptotic and proangiogenic properties. MSCs have the ability to fuse with somatic cells in vivo and depending upon the microenvironment, the fusion products may perform tissue-specific function. Stem cell factor (SCF) is one of the important cytokine for cardiomyocyte survival following cardiac injury. The main objective of the present study is to determine the fusion potential of SCF transfected MSCs and ca...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
... of homoharringtonine on proliferation and apoptosis of osteosarcoma cells through long non-coding RNA (lncRNA) LINC00857/microRNA (miR) -340. U2OS of osteosarcoma cells was treated with harringtonine at a concentration of 0.25, 0.5, 1, 2, 4 μg/ml. P21 and cysteine-containing aspartic protease 3 (Caspase-3) protein expression in U2OS cells were detected by western blot, thiazole blue (MTT) and plate cloning experiments to determine cell survival and colony ...

Jiao Ma*

...d and studied. Increased proliferation and differentiation of these cells in areas of cardiac ischemia is a significant factor in the repair and enhancement of heart injury. This role of endogenic cardiac stem cells can be regulated by factors. Numerous causes, such as paracrine and autocrine factors, extracellular matrixes and genetic factors. In general, it is well known that these variables could have an appropriate role to improve the effectiveness of the ...

Dabeeran Zehra1, Zahida Memon1, Shumaila Usman2,*, Almas Jabeen3, Kauser Ismail1 and Rehan Ahmed Siddiqui2

...associated with cellular proliferation, however the effects on cellular apoptosis were found to be insignificant.

...

 Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4

...increase in cell growth, proliferation and mineralization markers of osteoblast cells that increased with time as the highest concentrations of CeO2 innanobioactive glass (sample CL/CH/C10). Cell viability proved also that all scaffolds and their extracts showed proliferation inhibition with time < 25% reference to final cell number of control cells. Among the composites, having CL/CH/C5 showed the highest...

Sheng Dong1*, Ning Li2, Jiawei Zhang2 and Ting Wang2

...scurainia sophia (DS) on proliferation, apoptosis and endoplasmic reticulum stress of high glucose (HG)-induced mouse podocytes and its regulation of LncRNA KCNQ1OT1. In vitro cultured immortal mouse podocytes were divided into Con group, HG group, HG+DS-L group, HG+DS-M group, HG+DS-H group. si-NC and si-KCNQ1OT1 were transfected respectively into podocytes, followed by 30 mmol/L glucose intervention for 24 h. pcDNA, pcDNA-KCNQ1OT1 were transfected into podoc...

Xian Zhang1, Erjiang Lin1, Shizhe Hong1 and Zhengjie Zhu2*

...ransferase (SSAT) on the proliferation and apoptosis of prostate cancer LNCaP cells under hypoxic conditions. Prostate cancer LNCaP cells were transfected with the eukaryotic expression plasmid pcDNA3.1 (D)/V5-His-SSAT, and cultured at 1% oxygen concentration. Western blotting was used to detect expression of SSAT, Akt, GSK-3β and β-catenin proteins; and flow cytometry was used to detect cell apoptosis rate and mortality rate. SSAT was successfully t...

Wei Cui1, Yanwei Du1, Lijuan Jiang1, Yan Wei1, Yuguo Li1, Wenfeng Zhang1* and Ling Zhang2*

...el was established, cell proliferation and apoptosis were detected. The protein expression levels were detected by western blot. Rats with type 2 diabetes mellitus were fed high-fat-sugar diet and low dose of streptozotocin for 4 weeks to observe the body weight, fasting blood glucose, etc. The pathological changes of hippocampal CA1 region were observed through hematoxylin-eosin staining, and the expression of related proteins was detected. The results showed...

Feng Xu, Weijing Fan and Guobin Liu*

... rutin on ox-LDL-induced proliferation, apoptosis and oxidative stress of vascular endothelial cells. It is concluded that rutin had a protective effect on ox-LDL-induced vascular endothelial apoptosis and oxidative injury, and its mechanism may be related to the up-regulation ofNEXN-AS1/miR-410-3p pathway.
...
Noha M El-Motaily1, Ossama M Abdou1, Heba S Farag1, Kawkab A Ahmed2, Mahmoud Saber1*
... and caused by excessive proliferation of the normal skin commensal Demodex mites. Hemato-biochemical changes were previously reported in patient dogs with demodicosis due to the effect of stress caused by mites. This study was conducted on 38 dogs, 20 of them were used as control group and 18 were demodicosis infected dogs, for evaluation of hematological, biochemical and histopathological alterations. Beside detection of the antioxidant trace elements (Zn, C...

Rizwana Abdul Ghaffar* and Kamil Nadeem

...ecrosis and dilation and proliferation of blood vessels.

...

Pakistan Journal of Nematology

December

Vol. 39, Iss. 2, Pages 68-159

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