El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter

Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...(AIV) of subtype H5N1 in poultry since 2008. Since then, these viruses continue to evolve. In 2006-2008, outbreaks were reported mostly during the winter season, but in 2009-2012 they occurred year round. H5N1 AIV succeeded in infecting not only domesticated birds but also many other avian species and mammals. Infections of humans were accompanied by mutations in the hemagglutinin (HA) protein which improved the binding affinity to human receptors but simultan...

Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*

...ve been used in domestic poultry in China. But H9N2 AIVs occurred frequently and caused severe economic losses to the poultry industries. H9N2 AIVs can evade the pressure of vaccination by genetic evolution and antigen variation. Moreover, the ratio of L226 in the haemagglutin (HA) receptor binding site has increased, which indicates that the H9N2 virus has the increasing potential to infection and cause human epidemics. The...

Salma Shaheen, Muhammad Jamil Khan, Saleem Jilani

...compost @ 0.7 t ha-1 and poultry manure @ 5 t ha-1 were applied with half of the recommended dose of NPK @ (75:60:30 kg ha-1) with or without EM. In 2009-10, the application of EM with Press mud @ 20 t ha-1 significantly enhanced spinach growth by exhibiting higher average spinach plant height (35 cm), number of leaves (16.4), fresh foliage yields (330 g pot-1), dry foliage yields (32 g pot-1), leaf length (40.5 cm) and leaf area (238.4 mm2) relative to

Arshad Ali Khan1, Muhammad Sajid1, Abdur Rab1, Sahib Alam2, Abdul Bari3

...-50, 25-75 and 0-100% of poultry manure (PM) and Sul- phate of Potash (SOP). All the factors (K-sources, cultivars and years) alone and in combination sig- nificantly affected the growth, yield and fruit quality of tomato. The highest number of leaves plant-1 (75.25) and yield (49.25 t ha-1) were gained by Falcon in 50-50 treatment during, 2010. However, the plant height (134 cm) and fruit size (147.1 cm3) were higher at 25-75 and 75-25 treatment, respec- tive...

Rashid Saleem1*, Zammurad Iqbal Ahmad2, Muhammad Abbas Anees2, Abdul Razzaq3 and Ashiq Saleem1

 

...-80-60 NPK kg ha-1); T4, poultry manure (PM) @ 15 t ha-1 and half PM+ half PK+ inoculation, half poultry manure (7.5 t ha-1) + half PK (40-30 kg ha-1) + inoculation. According to statistical analysis, data revealed that plots treated with PK + inoculation with Rhizobium gave 33% higher mungbean seed yield over control. Similarly, higher values for yield contributing factors were also recorded in PK+ inoculation with Rhizobiu...

Aqeel Ahmad, Zammurad Iqbal Ahmed, Irfan Aziz*

 

...0-20 kg ha-1, poultry manure 20 tons ha-1, compost (press mud) 12.5 tons ha-1 and inoculation by phosphorus mobilizing microorganisms 2.5 packets ha-1, in addition to control were evaluated under lentil-sorghum and lentil-mungbean cropping patterns. Fertilizer treatments were only applied to lentil while their residual effect was studied on sorghum and mungbean. The results revealed that net benefits and marginal rat...

Imdad Sohu1, Allah Wadhayo Gandahi2*, Ghulam Raza Bhutto2, Muhammad Salim Sarki2, Rabail Gandahi3

...s of farmyard manure and poultry manure in combination with inorganic NPK fertilizers had shown positive effect on chickpea plant height, number of branches plant-1, number of pods plant-1 and seed index. The growth and yield of chickpea significantly increased with the collective application of chemical fertilizers and organic manures. It is concluded that the application of half of recommended dose of NKP (18-36-10 kg ha-1) in combination with

Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3

Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg

...ty measures, controlling poultry movements and inactivated vaccines were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectan...

 Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid

 
...e, Mn and Zn) present in poultry egg and meat were determined in the following three districts; Peshawar, Dir Lower and Malakand of Khyber Pakhtunkhwa by using atomic absorption spectrophotometer. In all the three districts the egg albumen was found to contain significantly higher levels of Pb, Cd and Cr as compared to egg yolk which contains significantly higher levels of essential elements (Fe, Mn and Zn). The mean concentrations of toxic heavy metals; Pb, C...

Metin Duru1*, Asuman Arslan Duru1, Köksal Karadaş2, Ecevit Eyduran3, Harun Cinli4 and Mohammad Masood Tariq5

... cartons and shipment in poultry industry. As a result, it was determined in the study that CHAID analysis may be used to better prove relationship mechanism between egg quality characteristics which are of great importance for higher price and more income of fertile and table eggs.

...

Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A

... pullets in a commercial poultry farm in Jos, Plateau State, Nigeria with mortality of up to 79.12%. The clinical signs observed were prostration, ruffled feathers, diarrhea, anorexia, trembling and mortality that lasted for 6 days. Postmortem findings include ecchymotic hemorrhages in the breast and thigh muscles, mucosa of the junction between the proventriculus and gizzard, severely enlarged and hemorrhagic bursa of Fabricius (BF) and splenomegaly. The burs...

Sania Subhan Qureshi, Mian Saeed Sarwar and Zahir Shah

...t-align: left;">Backyard poultry farming has been in practice in Peshawar and the surrounding regions. It provides a source of meat and eggs to the local population and helps in poverty alleviation and a source of family income through entrepreneurship. A hen with the complaint of swelling on abdominal region, came to Veterinary Teaching Hospital, The University of Agriculture Peshawar. Physical examination was performed and it was diagnosed a case of muscle ...

Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan

... important challenges to poultry production worldwide. That is in addition to its ability to induce mortalities and reduce the productivity, it accused to be negatively affecting the avian immune response facilitating the secondary infection. Many reports take into account the genotypic and pathogenic characterization of H9N2 in avian species. However little is known about the impact of different strains on the innate immune response. In the present study, usi...

Salma Shaheen, Mumtaz Khan, Muhammad Jamil Khan, Saleem Jilani, Zarina Bibi, Muhammad Munir and Mehwish Kiran

 

...1 compost (T3), 5 t ha-1 poultry manure (T4) and mixed chemical fertilizer in the ratio of 100 : 40 : 56 kg ha-1 as N, P and K (T5). Each treatment was applied alone and with EM. Results showed that EM + pressmud combination significantly improved soil physical and chemical properties as well as spinach growth compared to other fertilizers with EM. Maximum soil organic matter content (OM) and K was observed in treatment where pressmud was co-applied with EM. W...

Muhammad Afzal1 and Murad Khan2*

...omic analysis of broiler poultry farms, a survey research was carried out in district Lower Dir in 2015. Four tehsils were selected namely Adinzai, Balambat, Timergara and Sumerbagh on the basis of purposive sampling technique. From these tehsils, 92 farms were selected randomly from 460 poultry farms. The sample farms were categorized into three groups i.e. small, medium and large. For analysing the data, descriptive statis...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*
...mples collected from two poultry processing plants represented broiler carcasses swabs and worker (hand swabs and nail scrapings). Phenotype-based methods for identifying yeast especially Candida species are often difficult, time-consuming and not enough to identify most yeast species. . Molecular biological techniques provide a useful alternative approach. Concerning with genotypic identification, polymerase chain reaction-restriction fragment length polymorp...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
... commercial and backyard poultry.
...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2
...quent economic losses to poultry industry. Effected birds show lymphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of avian leukosis v...

Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1

...ssing several batches of poultry carcass.Several samples were collected from three poultry slaughterhouses within Jos metropolis at intervals for a period of 6 months to check for presence of Salmonella during various stages of processing. Salmonella was isolated from at least one of the four matrices in the three LBM with an overall prevalence of 20.2%. Of the 84 samples collected, 17 contained Salmonella. With regard to th...

Muhammad Zafarullah Khan

...airy, fruit cultivation, poultry, bee keeping and edible and non-edible like forestry products etc. An Agriculture Extension Officer (AEO) plays the role of a hub in agricultural development and covers all the areas of Agriculture. The present study was carried out in all the 24 districts of Khyber Pakhtunkhwa to investigate the agricultural mechanization competencies of AEOs. Sum total of 111 AEOs were contacted for data collection. Primary data were collecte...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...losses in the commercial poultry production sectors. Due to potential of H9N2 infections in human, LPAI H9N2 poses serious public health risk. Therefore, development of new potent and cost-effective influenza vaccines is urgently needed to safeguard poultry and to contain zoonosis. The primary goal of this study was to develop H9 plasmid-based DNA vaccine against the currently circulating AIV subtype H9N2 targeting HA gene o...

Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*

...infections in commercial poultry.

...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...icius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate at the rate of 0.1ml of EID50 (virus titer 105.50/100μl) on 2nd week of age thro...

Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2

...n vaccination regimen in poultry, a total of one hundred day old commercial broiler chicks were vaccinated with LaSota. A group of chicks (n=40) were immunized using baculovirus (H5 and ND) inactivated vaccine via subcutaneous route. Birds (n=20) were then either challenged with 106 EID50 using Egyptian avian influenza H5N1 and Newcastle disease genotype VII. Comparative blood profiling in vaccinated and challenged, non-vaccinated and non-challenged and non-va...
Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
...ub>2 prevalence in poultry population was directly proportional to temperature, season and elevation.
...

Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3

...he health of animals and poultry. In the current studies the citrus waste is dried and mixed with the broiler feed replacing maize from the feed at 0%, 2.5%, 5%, 7.5% and 10%. 225 birds were reared in 5 treatments and 3 replications of each treatment having 15 birds in each replication. The chicks were being provided with the standard conditions for light, water, vaccination and experimental feed for 42 days. The body weight gain, feed intake and the feed conv...
Oaj Khurshid1*, Naureen Aurangzeb1, Ayaz Khan1, Alia Naz1, Abdullah khan1, Sobia Nisa2 and Sehrish Sajawal3
...of yard waste or cow and poultry manure etc. For land reclamation or plant productions the use of compost may be supportive not only increase moisture holding capacity but also help to sustain soil conditioning. Composting through MSW could be introduced country wise as solid waste management option for the recycling and reuse of the organic remains.
...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

... commercial and backyard poultry production sectors. Owing to public health risk, effective control of the virus in imperative. DNA vaccination is a promising new approach for the prevention and treatment of many viral diseases because of its ability to induce both humoral and cellular immune responses against antigens encoded by recombinant DNA. The goal of this study was to construct Egy-H5 plasmid DNA-based vaccine against a currently circulating avian infl...

Murad Ali1, Farmanullah Khan1, Wiqar Ahmad2* and Imran Khan1

...y), four combinations of poultry manure (PM) i.e. 10, 7.5, 5 and 2.5 t ha-1 with 0, 25, 50, 75% of the RD (T7, T8, T9, T10, respectively) and four combinations of the mixed FYM (5 t ha-1) and PM (2.5 t ha-1) with 0, 25, 50, 75% of the RD (T11, T12, T13, T14, respectively) were evaluated in RCB design. Results revealed that T13 (integrated 5 t ha-1 FYM + 2.5 t ha-1 PM + 50% RD) resulted in the maximum grain and biological yield (4.2 and 11.2 t ha-1, respectivel...

Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4

...s economic losses to the poultry industry, these viruses pose a significant threat to public health. Owing to significant involvements of neuraminidase (NA) gene in virus pathobiology by mediating virus entry and release, the present study was conducted to determine the phylogenetic relationship of currently prevailing H9N2 isolates in Pakistan. A sum of ten H9N2 AIVs were isolated from 400 suspected samples and were confirmed through serological and molecular...
Furqan Sabir1, Muhammad Tayyab1,*, Bushra Muneer2, Abu Saeed Hashmi1, Ali Raza Awan1, Naeem Rashid3, Muhammad Wasim1 and Sehrish Firyal1
...he growth of monogastric poultry birds. Current study deals with the production and characterization of recombinant thermostable phytase from Thermotoga naphthophila (PHYTN). This study was an initial step for the fulfilment of domestic industrial requirement of phytase in Pakistan.The PCR resulted in the amplification of 1.8 kb phytase gene. SDS-PAGE confirmed the size of recombinant protein as 70 kDa. The optimization studies demonst...

Carolina Torres Alejo1,2, Laila Andreia Rodrigues Beserra1,2, Paulo Eduardo Brandão1,2, Luis Ramiro Luna Espinoza1,2 and Fabio Gregori1,2* 

... continued monitoring of poultry flocks to better understand its epidemiology and understanding the impact of one virus on the pathobiology of second virus 

...

Mohsan Ullah Goraya1,2, Liaqat Ali1,2 and Iqra Younis3  

...ignificant losses in the poultry industry. However, successful establishment of infection in the respiratory tract switch on the pathogen recognition by innate immune receptors and establish first line of defense against viral infections. When specific cellular receptors recognize a virus they trigger the translation of inflammatory cytokine and chemokines leading to establishment of an antiviral state of the host. In this review, we discuss the recent underst...

Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob

Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1 

 

...ontrol viral diseases in poultry, however, the interference of physico-chemical properties of the emulsions are overlooked when compared to antigenic properties. This study was designed to assess the effect of two different commercial inactivated water-in-oil multivalent vaccines; vaccine A contains IBV (Massachusetts M41 and D274 strains), NDV (Clone 30), aMPV (BUT 8544) and IBDV (classic strain) whereas vaccine B contains IBV (Massachusetts M41 and a Brazil ...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...nt disease in commercial poultry industry. This study aimed to develop and optimized a novel tPCR approach to detect Mycoplasma gallispeticum, Mycoplasma synoviae and nonpathogenic mycoplasmas at the same in a single PCR reaction collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods we...
Aqsa Imtiaz and Abdul Rehman*
...e soil contaminated with poultry waste. From amongst nine bacterial keratinase producing isolates were found. The BML5 isolate was identified as Bacillus subtilis based on biochemical characterization and 16S rRNA Ribotyping. B. subtilis BML5 showed maximal keratinase activity at 37°C and at pH 8. An increase in keratinase activity of B. subtilis BML5 was determined in the presence of Ca2+, Mg2+, and...
Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*
...siological activities of poultry oviduct and the molecular mechanism of sperm storage.
...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4

 

...major economic losses at poultry farms especially during during co-infections of respiratory pathogens. However, impact of co-infections is not well known, especially in broilers. The current study was aimed to assess the probable synergism of E. coli (O78) and velogenic Newcastle disease virus in the broiler model. Three-week-old commercial broilers were inoculated with either vNDV, E. coli serotype O78 or both agents simultaneously or 3 days ap...
Muhammad Aqeel Sarwar1*, Abid Ali2, Manzoor Hussain2, Saadia3, Muhammad Khubaib Abuzar4, Ijaz Ahmad5 and Sohail Latif6
... + 1.5 t ha-1 poultry manure(PM), (F4) 75% of recommended dose of NPK +3 t ha-1 farmyard manure (FYM) and(F5) 75% of recommended dose of NPK + 4.5 t ha-1 press-mud (PrM) along with seed inoculants (I1) Azospirillum and (I2) Azotobacter separately for each plot. Data regarding yield, quality and nutrients use efficiency parameters were collected and analyzed. Results...

 Rubina Bano, Hassnain Shah, Muhammad Sharif and Waqar Akhtar*

...cteristics of the sample poultry farmers along with cost and profitability analysis. Broiler farming was done by adult males on full time basis. It was the main source of income of a family. The average flock size in the study area was 4033 birds. The study also bifurcates the cost structure and fixed cost accounts for 7 % while variable cost accounts for 93% of total cost of production. Feed makes the major share and accounts for 49.34%. Broiler production in...
Saba Rafique1,2,*, Khalid Naeem1, Naila Siddique1, Muhammad Athar Abbas1, Aamer Ali Shah2, Akbar Ali1, Abdul Rahim1 and Farooq Rashid1
...onchitis from commercial poultry. Initially the isolated IB-virus was characterized by RFLP using enzymes Alu I, Hae III, BstYI and XcmI. On the basis of its distinct RFLP pattern from other known IBV vaccine strains, the isolate was named as KU145467_NARC/786_Pakistan_2013 (also named as Pak-786) was subjected to Spike gene sequencing covering the amino-terminus region of 01 to 974 base pairs. The S protein sequence was submitted to the GenBank with accession...

 Farrukh Saleem*, Babar Hilal Ahmad*, Saleem Zahid* and Bilal Kabeer*

COMPARATIVE PRODUCTIVE PERFORMANCE OF INDIGENOUS NAKED NECK AND NAKED NECK CROSSBRED LAYER CHICKENS

Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***

ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...ost important disease of poultry throughout the World is caused by Newcastle Disease Virus (NDV). It is causing huge economic losses in poultry industry of Pakistan. Regardless of vaccination, other prevention and control measures are necessary to prevent ND outbreaks. Natural resources have been exploited to obtain antiviral compounds in several latest studies. In this study, the antiviral activity of Viro Care GZ-08™...

 Ibrar Ali*, Abdul Mateen Khattak*, Muhammad Ali* and Kalim Ullah**

PERFORMANCE OF DIFFERENT TOMATO CULTIVARS UNDER ORGANIC AND INORGANIC REGIMES
...s. Organic regimes (FYM, poultry manure and mushroom compost) and inorganic (NPK) regimes were allotted to main plot, while cultivars (Roma VF, Roma, Super Classic, Bambino and Rio Grande) were subjected to sub plots. Organic and inorganic regimes significantly (P≤0.01) influenced all the studied attributes of tomato cultivars. Among different -1 cultivars, Roma gave maximum plant survival (93.8%), number of leaves plant -1 -1 (84.1), number of flower inflo...

Salar Khan Yousafzai*, Shah Masaud Khan*, Khalil ur Rehman**, Junaid Khan*, Sher Aslam Khan*, Ijaz Hussain* and Ishrat Naz***

RESPONSE OF TOMATO CULTIVARS TO DIFFERENT ORGANIC FERTILIZERS UNDER AGRO-CLIMATIC CONDITIONS OF MINGORA, SWAT
...nalyzed data showed that poultry manure -1 -1 gave maximum yield (24.65 tha ), followed by FYM (24.38 tha ) and mushroom -1 compost (24.11 tha ) while minimum was recorded in plots where no organic fertilizer was used. The results revealed that cultivar, Rio Grand showed maximum number of plant survival percentage (98.33%), days to flowering -1 -1 (40.73), number of flowers plant (6.23), number of fruit plant (25.67), fruit -1 3 weight (8.84 kg), number of lea...

Mehwish Kiran*, Muhammad Saleem Jilani*, Kashif Waseem*
and Muhammad Sohail**

EFFECT OF ORGANIC MANURES AND INORGANIC FERTILIZERS ON GROWTH AND YIELD OF RADISH ( L)
...manure (FYM) @ 25 t ha , poultry manure (PM) @ 10 t ha , goat manure (GM) @ 15 t ha , press mud (PrM) @ 20 t ha , sewage sludge (SS) @ 20 t ha and nitrogen, phosphorus, potassium (NPK) @ 120-65-100 kg ha . Data on leaf plant , leaf length (cm), fresh leaf weight plant (g), dry leaf weight plant (g), root length (cm), root diameter (cm), fresh root weight plant (g), dry root weight plant (g), total biomass plant (g), root yield pot (g) and root yield (t ha ) we...
Alaeldein M. Abudabos*, Abdullah N. Al-Owaimer, Elsayed O.S. Hussein and Mutahar H. Ali
...rinking water or feed of poultry has become a common practice. Broilers are more susceptible to heat stress as compared to other animals. The current experiment was conducted to examine the effect of commercial vitamin C supplement (VC100, 200 mg/kg), on performance of broilers. Two levels of environmental temperatures: normal (22°C) and high (32°C) and two levels of vitamin C in drinking water (with and without) were arranged in a factorial arrangemen...
Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*
...iratory disease (CRD) in poultry and pheasantry birds of Northern Pakistan is underreported. In this study we report on the seroprevalence and isolation of MG from a total of 2341 poultry and 24 pheasantry birds. Overall, seropositivity of 46.56% (1090/2341) and 27.2% (6/24) against MG was observed among poultry- and pheasantry- birds, respectively. Broiler breeder and broiler showed 58.93...
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...nd welfare in local poultry breeds. These findings are expected to provide a theoretical guide to choice of breeding methods for poultry enterprises.
...
Zahida Parveen1, Safdar Sidra1*, Bushra Nisar Khan2
...afowls were preferred to poultry feed but in present research, the diet preference was in order of maize >millet >poultryfeed. During feeding, the behavioral patterns of the captive peafowls were monitored on daily basis. Time spent in different behavioral activities was noted in seconds using the stopwatch. On average, most time spent by all species and varieties of peafowls was in walking around within the enclosure ...
Ligia Neves Scuarcialupi, Laila Andreia Rodrigues Beserra, Julia Rosas Hochheim, Rodrigo Martins Soares, Fabio Gregori*
...gative impact on egg and poultry production. The objective of this work was to detect and characterize Rotavirus F (RVF) and G (RVG) found in Brazilian commercial avian farms. A total of 97 intestinal contents was collected between 2008 and 2015 from 10 Brazilian states, and submitted to two different RT-PCR, targeting a fragment of viral VP6-coding nucleotide sequence of each viral group. Amplicons were further submitted to genetic sequencing and analyzed by ...

Murad Khan1* and Muhammad Afzal2 

...nt farm sizes of broiler poultryin district Dir Lower. Four tehsils, namely Adinzai, Balambat, Timergara and Sumerbagh were purposively selected during the year 2015. From these tehsils, 92 poultry farms were selected proportionately from total 460 poultry farms and data were collected on structured questionnaire. The sample farms were categorized into three groups i.e. small, medium and l...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...ler (n= 152; 60%), rural poultry (n=14; 6%), domestic desi birds (n=12; 5%), pigeons (n=9; 3.6%), desi chicken (n=9; 3.6%), broiler breeder (n=3; 1%) and layer (n=2; 0.8%). Overall sero-prevalence was 53% and average range of the hemagglutination inhibition (HI) antibody titer (MT log2) against AIV H9 remained 7±3. Real-time PCR results showed 8.3% (42/507) of the samples were positive for viral matrix gene. Sub-typing was performed by specif...

Mehran Ali, Inamullah*, Sajjad Ahmad and Arsalan Khan 

...(control, cattle manure, poultry manure, sheep manure, mushroom spent, mungbean residue and urea) in subplots. The results exhibited that, higher plant height (200.4 cm), grains ear-1(351), seed index (228.8 g), grain yield (3522 kg ha-1), and biological yield (10799 kg ha-1) were observed with nitrogen sources incorporated with mould board plough. In case of nitrogen sources; poultry manure (3507 kg ha-1), sheep manure (346...

Madeeha Alamzeb1, Shazma Anwar1, Asif Iqbal1,2*, Song Meizhen2, Mazhar Iqbal3, Sara4, Muhammad Ramzan2 and Afza Tabassum1
 

...urces (cattle, sheep and poultry manure each at 5 t ha-1) and nitrogen levels (0, 75, 100, 125 and 150 kg ha-1) on dry matter partitioning of wheat under tillage systems (conventional and deep tillage). The experiment was laid out in randomized complete block design with split plot arrangement, having three replications. Tillage systems and organic sources were used as main plot factor, nitrogen levels as subplot factor. The results revealed that organic sourc...

Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1* 

...eat and/or prevent NE in poultry. Further studies on the effect of EOs on other enteric infection, intestinal microbiota, poultry feed components, and poultry meat quality are required. 

...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
...ble limit (0.1 mg/kg) in poultry meat set by WHO and European Union. The estimated daily intakes (EDIs) of the tested metals through the consumption of the Baladi chicken were found to be lower than their respective reference oral doses (RfD) set by USEPA. The non-carcinogenic health risks to the target population due to exposure of the tested metals were assessed by estimating the Hazard Quotient (HQ) and Hazard Index (HI) values. The HQ and HI values observe...

Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair

...culating in the Egyptian poultry flocks during 2015 -2016 was conducted. Twenty one vaccinated chicken flocks suffering from different form of lymphomas, representing different governorates in the Egyptian Delta, were screened for MDV infection. Pathological findings in the visceral tumours and presence of viral DNAs with variable mitotic figures were observed in the examined visceral organs. Eight samples were screened positive for the major MDV-encoded oncog...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ed significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and gain a better understanding of the genetic properties of the FAdV strains responsible for recent outbreaks, the sequence analysis of hexon gene of 10 isolates associated with IBH and HPS was determined. Sequence alignment and phylogenetic analysis based on direct sequencing of hypervariable region of hexon gene grouped these sequences into two distinct gro...

Tanzim Ullah Khan1*, Mohammad Tariq Jan1, Ahmad Khan1, Gulzar Ahmad2, Muhammad Ishaq2, Khilwat Afridi2, Murad Ali2, Muhammad Adeel Qureshi2, Imtiaz Ahmad3 and Muhammad Saeed2  

... fertilizer nitrogen and poultry manure for wheat improvement at New Developmental Research Farm, Khyber Pakhtunkhwa Agricultural University, Peshawar in 2010-11. Three levels of fertilizer nitrogen (75, 100 and 125 kg ha-1) and two poultry manure levels (0 and 4 tones ha-1) were allotted to main plot while time of fertilizer nitrogen application were as: full at land preparation, full at boot stage and ½ at land prep...
Muhammad Anwar Iqbal1, Habib Rehman2, Ali Hussain3,*, Faiza Jabeen4, Ijaz Ahmad2, Kamran Ashraf5, Muhammad Irshad Arshad6 and Omaima Khan7
...n each group. Corn-based poultry feed (basal diet) was given to control group birds (Group A) and the same feed mixed with three different concentrations of MOS i.e., 0.1, 0.5 and 1.0 %, was offered for 35 days to the birds of experimental groups, B, C and D, respectively. Results showed that the birds of group C were heavier (P < 0.05) during 2nd and 3rd weeks compared to the other groups. Feed conversion ratio was not affected by the dietary...

Muhammad Faheem Jan1*, Asad Ali Khan1, Waqas Liaqat1, Haseeb Ahmad1, Muhammad Dawood Ahmadzai1 and Wazir Rehan

... organic source includes poultry manure (PM) while inorganic source includes sulphate of potash (SOP). Potassium (K) was applied to field in such an arrangement that 100, 80, 60, 40, 20 and 0% K was obtained from the organic source and the remaining was balanced form inorganic source for supplying a total K of 80 kg ha-1. Randomized complete block design with split plot arrangement was used. Hybrids were allotted to main plots and K ratios to subplot. Analysis...

Madiha Naz1, Qudrat Ullah Khan1*, M. Jamil Khan1, Obaid Ullah Sayal2, Asim Afridi3 and Aziz Ullah Sayal3 

...st in the pots recieving poultry manure either with Foliar NPK or NAA. The yield of tomato was found greater in the treatments recieving soil applied NP, which was statistically similar in the treatment recieving poultry manure with foliar NPK. The treatments in the current study have shown to influence the qualitative parameter of tomato as total soluble solids, while the water content and fruit pH were non significantly ch...

Ejaz Ashraf1*, Bushra Hassan2, Nadeem Anwar2, Hafiz Khurram Shurjeel3 and Shafique-ur-Rehman

...unities, and training in poultry farm management are the core requirements for economic prosperity of rural communities in the area. It is concluded that respondents were keen enough to get maximum opportunities to participate in economic activities in order to get financial independence and to promote rural business in the area. There is a dire need to invest in expert knowledge and vocational training opportunities to bring change in the skills level of the ...

 Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1

Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...nt infectious disease of poultry which cause
immune suppression and immune organs damage in broilers. Supplementations of
fish oil (FO) enhance the immune status and subside inflammation. This study was
design to observe the effects of Fish oil on lymphoid organs through
histopathology, growth performance and antibody response in H9N2 challenged
broilers. A total of 80 chicks were divided in to 4 groups A, B, C & D. Group...

Mehran Ali1*, Inamullah1, Muhammad Bilal2, Salman Ali1, Farooq Nawaz1 and Muhammad Owais Iqbal1 

...(control, cattle manure, poultry manure, sheep manure, mushroom spent, mungbean residue and urea) as subplot. The results exhibited that, improved bulk density, grain yield, harvest index and soil total N (STN) were observed when N incorporated with MB plough. In case of N sources poultry manure, sheep manure and mushroom spent gave at par yield with urea. However, STN and other soil physical properties were significantly hi...
Kalimullah Khan1,*, Hafsa Zaneb2, Zia Ur Rehman3, Hamza Maris4 and Habib ur Rehman1
...n for future research on poultry feed in Pakistan.
...
Muhammad Usman Ahmadand Ikram-ul-Haq*
... isolates from 30 intact poultry intestines were cultured and identified preliminary on the basis of microscopy and biochemical testing. E. coli strain EC4 was screened as best oxyrase producer with activity of 0.41±0.008U/mL/min with 41% reduction in dissolved oxygen at pH 7.5, temperature 37°C, 25mM lactate as H+ donor after 20 min. This strain was later on confirmed by 16S ribotyping procedure. Oxyrase was used for improved cult...
Sakhra Mahmood1, M. Younus1, A. Aslam1, A.A. Anjum2, S. Umar3, Aamerzish Mushtaq3 and M.L. Sohail4,*
...mance from livestock and poultry, proper detoxification of AFB1 in feed is mandatory. This study was designed to analyze the biological detoxification potential of Saccharomyces cerevisiae (SC) in experimental quails fed with AFB1 added diets. For this purpose 180 quails, divided in 3 groups were reared and fed with 3 different experimental diets having 0.5 mg/kg of AFB1 contamination and varying levels of SC (0.5, 1 and 2 mg/kg of feed). Another 120 bi...
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
...eding and the climate in poultry house on production performance in a commercial poultry enterprise with a hybrid approach. In accordance with this purpose, the hybrid approach has two main objectives: i) Determination of the effective period for which the output factors [(chicken survival rate (%), egg yield (%)] are optimized at the same time and the appropriate value ranges for the input factors [temperature (o...

Muhamamd Naeem1 and Murad Khan2* 

...asonal variations in the poultry industry. There were 400 poultry farms in district Nowshera but the preliminary survey showed 127 operational poultry farms scattered over in the study area. Out of total farms, 60 firms were selected randomly. To capture the seasonal variation and effects of different factors on seasonal fluctuations in the poultry indus...

 Asad Sultan1, Rabia Ali1, Rifat Ullah Khan2,*, Sarzamin Khan1, Naila Chand1 and Ambrina Tariq3

... for energy component in poultry ration. However, certain anti-nutritional factors hamper the bioavailability of nutrients and need to be addressed to avoid compromised birds’ performance. The present study assessed the impact of phytase on enhancing the nutrient retention and phosphorus utilization in two different sorghum cultivars fed to broiler. In an open sided house, a total of 160, 14-days-old broilers were allotted to four dietary bioassay treatm...

Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1* 

... is still a havoc to the poultry flocks and outbreaks have occurred even in vaccinated flocks. Commercial Newcastle disease virus vaccines can provide various levels of protection against challenge with different NDV genotypes, raising the importance of the relationship between vaccines and field strains. The aim of the current work is to evaluate the effect of moisture percentage and pH on the persistence of velogenic NDV genotype VII in the
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
... gene is more studied in poultry, and studies in wild birds are rare. To understand the antiviral site of the Myxovirus-resistance (Mx) protein in the evolution of different wild birds, 10 wild bird species, including Anas formosa, Anas crecca, Anas strepera, Mergus squamatus, Accipiter nisus, Buteo hemilasius, Buteo lagopus, Passer montanus, Psittacula roseata and Emberiza elegans, were sel...

Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1* 

...ignificant threat to the poultry industry. Bad management for poultry byproducts (e.g. manure) might be a reason for environmental pollution which contaminate the environment either chemically through haphazard usage of medications and or biologically through massive use of homologous/heterologous vaccines especially the live ones. The aim of the current work is to estimate the persistence time for velogenic Newcastle diseas...
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
...for its use in paper and poultry feed industry. Under the optimal conditions we could able to produce 48 U/mL of recombinant cellulase.
...

Muhammad Rehan1, Asim Aslam1, Muti-ur-Rehman Khan1, Muhammad Abid2, Shakeel Hussain3, Javeria Umber4, Ahsan Anjum1 and Altaf Hussain2* 

... outbreaks in commercial poultry throughout the world. Despite intensive vaccination, ND is endemic in Pakistan and locally known as Ranikhet. Wild birds are considered natural reservoirs of Newcastle disease virus (NDV) and some common resident or migratory wild birds are associated with outbreaks of ND in commercial poultry in Pakistan. Continuous isolation of new genotypes in Pakistan shows the evolving nature of the viru...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...As a result, majority of poultry isolates was susceptible to most of the tested antibiotics and highest resistance rates were detected for ciprofloxacin (2.8%) followed by ertapenem, imipenem, meropenem with equal resistance rates of 1.4% with CLSI interpretative criteria. However, analyzing of the same data according to EUCAST interpretative criteria resulted in higher number of resistant isolates (n=16). In this case, isolates showed resistance to amoxicilli...

Muhammad Sohail1*, Asad Sultan2, Said Sajjad Ali Shah3, Muhammad Sajid1 and Adnan Khan4

...tively higher than other poultry species particularly broilers.

...

Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassan 

... and widely in different poultry sectors in Egypt causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, 1026 confirmed positive H9N2 cases by RT-PCR out of 23182 different examined samples of different species and sectors in Egypt, with a prevalence rate 4.4% by the year 2015/2016. However, the LPAI H9N2 showed a wide...

Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan  

...ly in different Egyptian poultry sectors causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, a total of 23182 cloacal and tracheal samples were collected from suspected cases between 2015 and 2016 and submitted to the Reference laboratory of veterinary quality control on poultry

Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan 

...ly contagious disease in poultry, also considered as a major threat for commercial and traditional poultry industry in Egypt, even in vaccinated flocks. A total of 8121 different samples (organs and swabs) that represented 588 NDV suspected flocks were collected from poultry farms showing respiratory manifestation and/or drop in egg production during 2015-2018. Out of 588 NDV outbreaks 95 ...

Zafar Abbas1*, Muhammad Mubashir1, Umair Riaz1, Zeenat Javid1, Muhammad Ashraf1, Saeed ur Rehman1, Muhammad Javid Qamar1, Syed Ali Zulqadar1 and Shahzada Munawar Mehdi2 

...zers like kitchen waste, poultry manure and compost were used while the inorganic sources were NPK fertilizer. This experiment was laid out in the field area of soil and water testing laboratory for research, Bahawalpur in growing seasons from July to October of 2017. Treatments were arranged in Two factorial Completely Randomized Design CRBD fashion with three replications and six treatments. Treatments are arranged as control T1 (Without fertilizer + No

Pakistan Journal of Agricultural Research

June

Vol. 32, Iss. 2, Pages 218-415

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