Sandeep Satapathy and Rupam Ghosh
...en identified as crucial mediators for clearance of influenza by up regulating several intracellular defense mechanisms. Associated with IL-6 signalling is the expression acceleration of sequestrome 1(SQSTM1) / aggrosome and colocalization of HDAC6 to the cytoplasm. The key player HDAC6 is investigated for modelling several intracellular processes that come into action to bring out the viral clearance. The shuttling of HDAC6 takes place from nucleus to cytopla...
Ye Liu2, Yulong Cong2, Yiming Shao1*
...imizing immune responses mediated by vaccines. These examples of selected studies will provide researchers some implications and inspirations for using HIV Tat to optimize immune responses of vaccines....
Derk Pereboom
...pulation is direct and immediate, the agent is not morally responsible. Fischer’s thought is that Plum’s non-responsibility in Case 1 can be accounted for by the conditions on mechanism-ownership, a feature of the compatibilist account of moral responsibility he and Ravizza (1998) developed, which, he correctly points out, I neglect in favor of the reasons-responsiveness component....
Muhammad Irshad1, Abdur Rab1, Javed Rahman2, Muhammad Sajid1, Ilyas Khan2, Sajad Ali1, Muhammad Razaq1, Sallahuddin1
...erent planting dates and media on the growth of kiwi (cv. Hayward) cuttings at Agriculture Research Institute (ARI) Mingora Swat, during 2011. The experiment was laid out in Randomized Complete Block Design (RCBD) with split plot arrangement in three replications. Planting Dates (20th Jan, 30th Jan, 10th Feb, 20th Feb and 2nd March) were allocated to main plots, whereas soil media (Silt+ Garden soil+ FYM) at the rate of 1:1:...
Dallin D. Oaks
...reporting a sudden and immediate confusion of languages, or even a sequence in which a confusion of languages led to a scattering of the people. Indeed, a close examination of the account seems to allow an interpretation of events that is compatible with what linguists have observed about how languages can diversify, though some challenges may still remain in reconciling assumptions about the available post-Babel time frame versus the lengthy time frame that l...
Muhammad Shah Zaman1, Ghulam Muhammad Ali2, Aish Muhammad1, Khalid Farooq2, Iqbal Hussain1*
...be used in Agrobacterium-mediated gene transformation of AtNHX1 to improve their tolerance against salinity stress....
Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...ns which are essentially mediating protective immunity, may explain the extreme infectious nature of the virus and its host-specific pathogenesis. Moreover, understanding the nature of such circulating field viruses is essential to underpin the endemic potential of PPRV and its possible spread to the susceptible wild or domestic small ruminants....
Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...c, were cultured on agar media and tested against Meloidogyne hapla both in-vitro and in-vivo. All fungi proved to be efficient in reducing final population of northern root knot nematode M. hapla and giving vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L....
Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3
...s grown on two different media, i.e. Potato Dextrose Broth (PDB) and Glucose Nutrient Broth (GNB) media for active secondary metabolites. Ethyl acetate (EtOAc) extract of PDB medium gave significant results against selected phytopathogens compared to GNB medium and selected for further study. Crude (1 µg.mL-1 ) obtained from PDB medium showed 2.50 ± 0.64 mm and 1.97 ± 0.21 mm inhibition zone against X. ca...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...lpation of the ligament, medial patellar desmotomy was performed in lateral recumbence, under local anesthesia achieved by 2% lignocaine hydrochloride. The skin was apposed with simple interrupted sutures using silk. Post-operative care was continued for five days. Clinical signs abated in all operated animals, proving that the technique employed was effective and of simple execution for the treatment of dorsal patellar fixation in bovine.
...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...me were sectioned in the median sagittal plane and histological slides of the worms were prepared for detailed morphological studies. Bile tissues, aspirates and blood samples of the same animals were analysed. The infection was found prevalent throughout the year but during post monsoon season it was relatively high. Morphological identification of G. expalanatum was carried out on the basis of size and shape of worm. The hematological and bile biochem...
Waqar Islam*1, 2, Madiha Zaynab3, Muhammad Qasim2 and Zujian Wu1,2
...al RNA silencing, R-gene mediated resistance and host factor related recessive resistance are categorized as most beneficial plant defense approaches used by plants. The review also briefly explains about introgression of durable resistance to generate virus resistant cultivars for economically important crops through molecular breeding techniques via utilizing advanced molecular markers involving cis and trans genetics. The review adhere recent research findi...
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
...eria were cultured in LB media and further the culture of each bacterium was divided into three parts, the first part was centrifuged in order to collect supernatant. While the second and third parts were also centrifuged but however supernatants were discarded to get their pellets, which were dissolved in normal saline. Of this one part was taken for thaw and freeze treatment, while the second for ultrasonication treatment, before conducting antimicrobial act...
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...and other DNA polymerase-mediated assays, and microarray detection. Of these, microarray detection provides the greatest capability for parallel yet specific testing and can be used to detect individual or combinations of viruses with sensitivity comparable to ELISA. Methods based on PCR provide the greatest sensitivity among the listed techniques but are limited in parallel detection capability even in “multiplexed” applications. Better, easier an...
Sitansu Pan and Amrita Das
Evaluation of shelf life of some value added organic formulations of Trichoderma harzianum
.... Among the four organic media, vermicompost retained 21.3 x 10 c.f.u./g at 120 days where as leaf mold,FYMand rice bran retained 20.1 x 10 c.f.u./g, 17.3 x 10 c.f.u./g and 15.1 x 10 c.f.u. /g of substrate respectively at 120 days of incubation. When oilcake was amended with organic material, vermicompost + mustard cake produced high population of the antagonist upto 60 days....
B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran
Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
... is grown in inexpensive media like molasses and yeast medium in deep tanks on a commercial scale. Biomass from the liquid fermentation can be made into different formulations like, dusts, granules, pellets, wettable powders. Trichoderma formulations can be applied to the seed either by dry seed treatment or by seed biopriming for control of several soil-borne diseases of some field crops. Similarly, seedlings of horticultural crops and rice are treated by dip...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...bacteria isolates in PDA media plates. In in-vivo test soil was amended with antagonist and wilt fungal and bacterial isolates 2 x 106 CFU/ml and 2 x 108 CFU/ml respectively as talc formulation @ 0.2% (w/w). Twenty one days old tomato seedlings were transplanted after dip treatment in antagonist fungal and bacterial CF in earthen pots filled with amended soil. Seven days after transplanting, the seedlings were inoculated with second stage juveniles of M. incog...
M. S. A. Mamun and M. Ahmed
Integrated pest management in tea: prospects and future strategies in Bangladesh
...es ago with tremendous immediate economic gains but its abuses were not foreseen or ignored. As a consequence there arose the development of resistance to pesticides, pest resurgence and undesirable pesticide residue in the made tea as the major problems. Current trends in eco-friendly insect pest management practices emphasize the host plant resistance, preparation and application methods of new botanicals and microbial pesticide formulations, evaluation of f...
Gadeeyya G and Ratna Kumar P.K.
... different fungal growth media. The diagnostic characteristic features of each isolate were critically studied using relevant literature. The pathogenicity of the isolates was examined to select biocontrol agent against host weed.
...
Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4
...in virus pathobiology by mediating virus entry and release, the present study was conducted to determine the phylogenetic relationship of currently prevailing H9N2 isolates in Pakistan. A sum of ten H9N2 AIVs were isolated from 400 suspected samples and were confirmed through serological and molecular assays. The sequence analysis of NA genes shown 99% homology with the H9N2 AIV recently isolated from Pakistan and their phylogenetic analysis revealed that all ...
Attaullah Khan1, Muhammad Shafi1*, Jehan Bakht2 and Shazma Anwar1
...tudied in sand as growth media. The results revealed that incremental increase of salinity stresses had decreasing effect on growth parameters compared with control. Highest germination (85.42% and 84.31%) was recorded from wheat varieties (Lalma-2013 and Pirsabak-2005). Minimum germination (64.40% and 73.89%), tillers plant-1(2.83 and 3.49), leaves plant-1(11.44 and 12.79), leaf area (21.47cm2 and 25.20cm2) and more days to emergence (9.92 and 8.98), was reco...
Raza Ullah1*, Qaisar Shah Safi2, Ghaffar Ali3 and Irfan Ullah3
...s middlemen (market intermediaries) involved at different stages in the marketing of citrus. This study is an attempt to examine the marketing margins of the intermediaries involve in the value chain of sweet oranges of citrus group. Three villages in Bunir district are randomly selected and a total of 120 sampled respondents are selected using proportional allocation method. The findings revealed that majority of the farmer...
Jawad Anwar* and Zafar Iqbal
...ompared to other culture media (Potato dextrose broth and yeast extract broth). Similarly maximum zone of inhibition was observed in optimized parameters like shaking flask culture, 25 °C temperature, media of pH 5 and light fermentation.The EtOAc extract exhibited the minimal value of MIC (10.41±4.50 µg/mL) against gram negative E. coli, while its acetonitrile fraction yielded (6.50±2.25 µg/mL)...
Ambreen Butt1, Uzma Malik2, Khadija Waheed3, Amna Khanum3, Samar Firdous4, Sara Ejaz5 and Fawad Randhawa2, Tania Shakoori6*
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...in MS and Chu’s N6 media produced 53.33 and 35.56% embryogenic calli, respectively. Inclusion of 5 mgL-1 silver nitrate in embryogenic medium enhanced the production of type II embryogenic calli by 54.90% compared to control treatment. Maximum regeneration (58.33%) was observed when embryogenic calli were placed on N6 medium supplemented with 60 gL-1 sucrose and 1 mgL-1 NAA for one week initially in dark and then three weeks on MS medium augmented with 2...
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
...the vascular network, is mediated through VEGF/VEGFR activation of the signal transduction pathways. Thus blocking VEGF is an efficient strategy for blocking tumor angiogenesis. Several anti-VEGF drugs have been developed over the last two decades and some are still at different development stages of pre-clinical and clinical trials. In this study, we explored the anti-angiogenic properties of three anti-VEGF molecules such as two fusion proteins, VEGF TrapR1R...
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
...ady Finger-2 showed intermediate resistant reaction while KT-458, Bhindi Punjab Selection, Arka Anamika and Bhindi Sabazpari proved to be comparatively resistant. Maximum fruit and shoot infestations were recorded on comparatively susceptible cultivars while the infestations were the minimum on comparatively resistant cultivars. As comparatively resistant cultivars viz. KT-458, Bhindi Punjab Selection, Arka Anamika and Bhindi Sabazpari suffered less damage and...
Ümit Kumbıçak, Zübeyde Kumbıçak* and Gülnare Huseynli
... X2 were intermediate in the karyotype.
...
Muhammad Siraj-ud-Din1,2, Riaz Aziz Minhas1, Usman Ali3,*, Mayoor Khan2, Muhammad Siddique Awan1, Nuzhat Shafi1 and Basharat Ahmad1
... 9.69%), Ephedra intermedia (n=25, 8.65%), Pistacia khinjuk and Ephedra gerardiana (n=23, 7.69%). Out of 36 plant species, 15 were consumed during summer (June to August), 10 in spring (April-May), six (6) in autumn (September-October) and five (5) in winter (February-March). Conservation of threatened Ladakh urial could be achieved by protecting its potential habitat and preferred food plant species.
... 
Rana Hanan1*, Muhammad Waqar Shafi1, Sumair Anwar2 and Numrah Nawaz1
Naeem Iftikhar1, Qamar Zaman Qamar2, Usman Ali3, Muhammad Siddique Awan4, Syeda Shaista Bibi4, Riaz Aziz Minhas4*
... study recommended the immediate formulation and implementation of Cheer pheasant conservation action plan along with declaration of its potential habitats as protected area to ensure the further survival of this important species....
Bushra Mazhar1, Nusrat Jahan1, Nazish Mazhar Ali1, Saiqa andleeb2, Shaukat Ali2*
...idues of rice bran based media. E. hormaechei produced 5.2 g/l of vanillin in culture media containing ferulic acid as a substrate. Optimum pH for E. hormaechei was 7 and the temperature was 30°C. Using optimum growth conditions and suitable fermentation medium, E. hormaechei can be used at an industrial level as producer of vanillin. Bioconversion of waste residues of rice bran oil to vanillin by bacterial strains is of...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
Aneeza Jamshed1* and Syed Amir Gillani2
...less penetration of mass media. To develop the awareness of hepatitis C among primary grade students. A total of 150 students of primary grade among 300, of Government sector Middle School Lahore were selected through multistage cluster sampling. A quasi-experimental study was conducted in the time period of 6 months from April 2014 to Sep 2014. Data analysis was done with SPSS 20. After describing the important variables, average percentages were used to find...
Ahmed Aziz Kurd, Amanullah, Saifullah Khan, Basharat Hussain Shah and Munir Ahmed Khetran*
... treated cuttings were immediately planted in polyethylene tubes (10 cm x 20 cm) filled with a sandy loam soil. In the control, the cuttings were planted directly in polyethylene bags without IBA application. Plants were kept in a shade house and humidity was increased manually by hand sprinkling (four times in a day). Highest rooting percentage (60%) was obtained in the cuttings treated with 3000 ppm. The maximum average number of roots per cutting (4.443) an...
Sana Qanber Abbasi1*, Ejaz Ahmed2 and Rabia Sattar1
... 3 cc was drawn from the median cubital vein after securing aseptic measures. The blood sample was allowed to clot for 30 minutes and serum was separated. The serum was then centrifuged andpro BNP levels were estimated by ELISA using Human pro BNP kit. Patients’ history and recorded blood pressure was documented on a questionnaire. The results were analyzed using SPSS 21. One way ANOVA and Independent sample T test were applied to compare the proBNP leve...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...ntrol group fish on both media nutrient agar (NA) and eosin methylene blue (EMB) viz. 1.8×103 and 1.56×103 (cfu ml-1) in gut contents whereas on skin only total bacterial count (0.36×103)was observed and no coliform bacteria were present while least CFU’s were counted in group 2 among skin 0.14×103 cfu ml-1 and gut 0.10×103 cfu ml-1
Nazish Shaukat1, Muhammad Javed1,Faiza Ambreen2* and Fariha Latif1
...l parameters of the test media viz. pH, dissolved oxygen, carbon dioxide, total hardness, calcium, magnesium and total ammonia were monitored on daily basis. Significantly increased peroxidase activity was observed in gills and liver of Labeo rohita after exposure to PbCl2 due to all doses as compared to the control. All results were statistically significant at p<0.05. Fish liver exhibited significantly (p<0.05) higher activity ...
Farhat Ullah Khan, Nowshad Khan and Zaibunnisa Abdullah*
Corresponding author: farhatkhan7@gmail.com (This is part of Ph.D. work of senior author)
IMPACT OFKINNOW (MANDARIN) YIELD LOSSES ON SOCIOECONOMIC CONDITIONS OF THE FARMING COMMUNITY IN PUNJAB, PAKISTAN
...ies of study area. The immediate benefit of this study will go to kinnow growers of Tehsil Sargodha and ultimately the economic growth of the country will be increased in future by improving the cultural practices and adopting modern post harvest technologies of kinnow throughout Pakistan....
Khalid Mahmood Qureshi, Fakhar ul Hassan, Qamar ul Hassan, Usman Shaukat Qureshi, Saman Chughtai and Adnan Saleem*
IMPACT OF CULTIVATION SYSTEMS ON GROWTH AND YIELD OF STRAWBERRY (FRAGARIA ANANASSA) CV. CHANDLER
...d i.e., standard growing media in polyethylene bags (T ), soil less media (T ), high tunnel (T ), green house (T ), 1 2 3 4 including open field condition on ridges (T ) by keeping plant to plant 5 distance of 30 cm and row to row distance of 60 cm. The study was conducted to evaluate the effects and suitability of different cultivation systems for better vegetative growth, production and fruit quality of strawberry. The res...
Sonila Hassan, Abid Hussain*, Muhammad Azeem Khan** and Irfan Mahmood*
RURAL-URBAN RETAIL PRICES AND MARKETING MARGINS OF FRESH FRUITS AND VEGETABLES IN PAKISTAN
.... The net margin to intermediaries for potatoes, onion, persimmon, pear, banana, sweet lemon, and guava was accounted at 43%, 111.4%, 316.5%, 104.5%, 353.9%, 370.8% and 152.4%, respectively....
Sajida Taj*, Muhammad Tariq Iqbal Khan**, Mazher Abbas and Arshed Bashir***
PRICE SPREAD AND MARKETING MARGINS OF CUT ROSE IN PUNJAB, PAKISTAN
...and other marketing intermediaries are quantified along the existing two marketing channels for cut flowers. In the first channel the main players were producers, wholesalers-cum-commission agents, retailers and consumers. In the second channel producers were directly linked with retailers. In Channel-I the highest consumer's price of Rs. 444.78 per 100 pieces of cut roses indicated that consumers have to pay more there. The price spread was greater in Channel...
Sara Khalid*, Khalid Mahmood Qureshi*, Ishfaq Ahmad Hafiz*, Khalid Saifullah Khan* and Usman Shaukat Qureshi*
EFFECT OF ORGANIC AMENDMENTS ON VEGETATIVE GROWTH, FRUIT AND YIELD QUALITY OF STRAWBERRY
... included T = planting 1 media -1 (soil + silt + farm yard manure); T = planting media + 400 mgl humic 2 -1 acid; T = planting media + 200 g kg leaf manure; T = planting media + 200 3 4 -1 -1 g kg vermicompost; T = planting media + 200 g kg plant fertilizer and T = 5 6 -1 planting media<...
Rabeea Tariq*, Khalid Mahmood Qureshi*, Imran Hassan*, Muhammad Rasheed* and Usman Shaukat Qureshi*
EFFECT OF PLANTING DENSITY AND GROWING MEDIA ON GROWTH AND YIELD OF STRAWBERRY
...e requirement of special media is very important step because plant growth is largely depended on the physiochemical properties of the growing media used. Winter strawberry production in a greenhouse using high plant densities and various media may be a viable alternative to openfield production system. Planting density can be increased thrice by using different production systems. Studies...
Abid Hussain*, Khalid Mahmood Aujla* and Sonila Hassan**
PRODUCTION AND MARKETING OF CAMEL PRODUCTS IN SEMIDESERT AND DESERT AREAS OF PAKISTAN
...rmers and 17 market intermediaries. It is found that both camel farmers and market intermediaries were less educated. It is observed that markets for camel milk, meat, hides and hair are less established in semi desert and desert areas of the country. Mean production of milk per farm household was 5.4 and 6.5 liters per day in summer and winter seasons, respectively; however, none of the surveyed farmers reported milk sale. ...
Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*
COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
...inations in regeneration media were studied to determine the best combination for in vitro morphogenesis of grapevine accessions viz., 4494(1) Kowar Sufaid, 4472(12) Harker Agoon and 4492(1) Kowar Naray. When levels of benzylaminopurine (BAP) were increased in the basal MS media for culturing the grape plantlets, there was a corresponding increase in the number of shoots and root mass. Additionally, the shoot proliferation r...
Muhammad Ali*, Ghulam Mustafa Sajid**, M. Faisal Anwar Malik*** and Kalimullah****
ESTABLISHMENT OF IN VITRO CULTURE OF GRAPES
...zed and tested on 75% MS media for germination and initial growth parameters. Accession No. 020017 (Dakh-1) exhibited highest viability (100%), shoot length (4.12 cm) -1 and nodes plant (3.8). Moreover, it was found that response of cultures to different treatments was dependent both on accession and treatment duration. In conclusion, this protocol proved to be useful in optimizing the dose and duration of the treatment of grape explants with the surface disin...
Khalid Mahmood Aujla* and Abid Hussain*
MARKETING SYSTEM OF LIVE CAMELS IN THE DESERT ECOLOGIES OF PAKISTAN
...lage dealers/market intermediaries. The regulatory laws of livestock markets applied in Pakistan are not comprehensive and do not cover all the aspects necessary for the markets to function efficiently. It is, therefore, recommended that the proper marketing system and physical infrastructure for live camels should be developed to benefit the poor camel farmers....
Tasawar Sultana*, Farah Deeba* and S.M. Saqlan Naqvi*
HIGH-THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION OF MEDICAGO TRUNCATULA IN COMPARISON TO TWO EXPRESSION VECTORS
...o efficient Agrobacteriummediated transformation for a long time. The selection of Medicago truncatula as a model legume plant for molecular analysis resulted in the development of efficient Agrobacterium-mediated transformation protocols. In current study, M. truncatula transformed plants expressing OsRGLP1 were obtained through GATEWAY technology using pGOsRGLP1 (pH7WG2.0::OsRGLP1). The transformation efficiency of this ve...
Abdul Rehman Khan*, Muhammad Mahmood–Ul-Hassan**, Rizwan Ahmad** and Anjum Munir ***
ARSENIC III TOLERANCE POTENTIAL OF FUNGI ISOLATED FROM POLLUTED AND NON-POLLUTED SOILS OF PAKISTAN
...significant role in bioremediation due to their large surface area and metal sorption capacity. In present study, arsenic (As) tolerance of indigenous filamentous fungi was explored. Fungal strains were isolated from peri-urban soils of Multan and Gujranwala irrigated with untreated industrial or municipal effluent. Some fungal strains were also isolated from non-polluted soils of Islamabad area. Arsenic (III) tolerance potential of 18 fungal isolates was test...
Aitezaz Ahsan1*, Muhammad Usman1, Ihsan Ullah2, Aamer Bin Zahur3 and Adnan Rasheed Malik4
...verse transcriptase loop mediated isothermal amplification over the last decade. These diagnostic assays vary in their sensitivity, specificity, reliability and reproducibility. Although these assays are detecting PPRV with precision but there is need to develop some cost effective diagnostic assays which would be applicable in the field conditions and in the developing countries like Pakistan.
...
Filiz Kutluyer*
...i>. Different activation media (NaCl, 0.3%; NaHCO3, 1%) were supplemented with L-tryptophan [Control (0), 0.5, 1, 2, 3, 4 and 5 mM] for assessing sperm motility and its duration, fertility and hatching rate of eggs. The results from the present study indicated that addition of L-tryptophan increased the motility rate and duration in O. mykiss, S. rizeensis and S. coruhensis compared to control group. Highest sperm motility rate ...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
...4257;ed from the culture media. The immunogenicity of the protein was studied in an animal experiment on mice, in which mice were injected subcutaneously. These findings suggest that the truncated Sf200 expressed in the pET-32a (+) prokaryotic vector can be used as antigen to detect antibodies against IBV.
...
Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*
...on modified fray’s media indicating typical mycoplasma like colonies with appearance of fried egg or nipple like characteristic of 0.1 to 1 mm in diameter with a dense raised center in the middle. Interestingly, culture positivity for broiler samples was found higher (36.8%) as compared to breeders (33.5%) and layers (29.5%), respectively. Overall, of the cultured samples, 71.19% (215/302) were confirmed as M. gallisepticum by specie specific PCR....
Ejaz Ashraf1*, Hafiz Khuram Sharjeel1, Raheel Babar2, Muhammad Junaid1, Qamar Iqbal3, Rabia Rasheed1 and Nosheen Fatima1
...verage, less use of multimedia, unavailability of training facilities to EFS, manifold duties assigned to extension officers and top-down dimension of planning of implementation of extension approaches were main weaknesses. The steps like effective evaluation of extension system, use of democratic nature extension approaches, and training facilities to EFS could improve working efficiency of Extension Field Staff and extension approaches thereby. ...
Muhammad Tariq Zahid1, Farah Rauf Shakoori2, Soumble Zulfiqar3, Khalid A. Al-Ghanim4 and Abdul Rauf Shakoori3,4*
...nological tools for bioremediation.
... 
