Simon Mwangi Kihu1*, George Chege Gitao1, Lily Caroline Bebora1, Njenga Munene John1, Gidraph Gachunga Wairire2, Ndichu Maingi1, Raphael Githaiga Wahome1, Davis Njuguna Karanja1, Julius Otieno Oyugi3, Ernest Lutomia3
... Clinical outcome, gross lesions and histological observations were suggestive of PPRV infection, which were confirmed by the application of a reverse transcription–polymerase chain reaction (qRT-PCR). Collectively, results indicate the continuous persistence of PPRV in Kenya....
Muhammad Arif Zafar1, Murtaz-ul-Hasan1*, Aayesha Riaz1, Asim Shamim2, Muhammad Farooq Iqbal1 and Arfan Yousaf
E-mail | murtazhassan@yahoo.com
... partial excision of the lesions. To this end, nine animals were selected clinical and sporadic papillomas different body parts. These animals were divided into three groups; Group A animals were operated for partial excision of the lesions, Group B were inoculated with autogenous vaccine alone and Group C were treated with inoculation of autogenous vaccine supplemented with immune-modulators (Levamisole and Vitami...
Nadeem Khan1*, Arshad Ali Khan1, Mukhtar Ahmad2, Muhammad Nouman2 and Badshah Islam1
...and Arnold Blood, lesser lesions were found. On the basis of results and overall performance Tarocco and Hamlin performed best in all aspects while Arnold Blood and Cara Cara revealed poor growth. ...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
... diarrhea and ulcerative lesions in the oral cavity. A total of eighteen goats from a herd of sixty, were affected and two goats succumbed within two weeks. Interestingly, the disease was exclusively observed in goats and all sheep kept in the same herd were serologically positive but did not show any clinical signs of PPR. The active PPR virus (PPRV) infection was confirmed by antigen capture ELISA and RT-PCR in both swab and body tissue samples. The molecula...
A. Sajeena and T. Marimuthu
Efficacy, stability and persistence of Ganosol, a Ganoderma based fungicide against plant pathogens
...ay reduced the number of lesions produced by Groundnut Bud Necrosis virus in cowpea by 88.39% when compared to the inoculated control, suggesting the antiviral potential of the formulation. ...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...ed and histopathological lesions observed in bursa, spleen and thymus and compared with control. Mild to severe hemorrhages and lymphocytic depletion, follicle necrosis, leukocytic infiltration, cyst formation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymph...
Vladimir Avdalovic1, Marijana Vucinic2, Radmila Resanovic3, Jelena Avdalovic4, Danka Maslic-Strizak5 and Milos Vucicevic3*
...ere FPD was high. Severe lesions were significantly (p<0.01) more frequent (80%) in broilers reared on chopped wheat straw than on pelleted straw (56%). Although the high moisture content was measured in both litter substrates and the high frequency of severe FPD was estimated in both groups of broilers, pelleted straw showed lower moisture content, occurrence and severity of FPD (2.56±0.19) comparing to chopped wheat straw (3.20±0.13) at the ...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
...ameness. The major gross lesions were erosive stomatitis, hemorrhages in lung, liver, abomasum, small and large intestine. PPRV NP was detected in sheep (n=20) and goats (n=18), anti-PPRV NP was also detected among sheep and goats (n=162) by ELISA, 3ABC-FMD enzyme immunoassay were positive (n=6) when ovine sera were tested. The study revealed that PPR-FMD viruses were circulating in the region and warrants proper control measures.
...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4
...were inspected for gross lesions. Samples of the respiratory organs (trachea, lungs, and air sacs) were taken for histological analyses. All the infected subjects showed clinical signs of varying severity. Co-infected groups showed the most obvious clinical signs, associated with significant higher mortality and respiratory organ abnormalities, in comparison with the mono-infected groups (P<0.05). There was a non-significant (P>0.05) effect of the inocul...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...re isolated from carious lesions on Mitis Salivarius Bacitracin agar along with twenty potentially active Streptomyces strains were isolated from the soil of agriculture farmlands. The biochemical and physiological characterization along with the molecular identification by 16S rRNA gene sequencing confirmed that caries isolates are pathogenic S. mutans and soil isolates belong to the genus Streptomyces. The antibiotic resistance profile o...
Riffat Mehboob1*, Shahida Perveen2, Naseer Ahmed3
...evalence of inflammatory lesions, benign neoplasms and their age-wise comparison.
Methodology: The study was carried out at Pathology Department, King Edward Medical University, Lahore. Data of 368 cases of BBD during a time span of 3 years (2011-2014) was obtained retrospectively.
Results: There were 190 patients of fibroadenoma (FA), 81 of fibrocystic disease (FCD), 64 of breast abscess (BA), 12 of granulomatous ma...
Maaz Ahmad1*, Mussab Ahmad2 and Tehreem Munir3
...ce of structural organic lesions. Approximately 15-20% of the general population in many developed countries suffers from dyspeptic symptoms at any time in a year, whereas in Pakistan, 14-22 % of general population suffers from FD.
Objective: This study was designed to evaluate the effectiveness of carica papaya extract (CP) in preventing functional dyspepsia in human volunteers.
Methodology: A community based,...
G. Venkatesan* and Amit Kumar
...h regions and rarely the lesions are found in other parts of the body and internal organs. Another characteristic feature of ORFV including other PPVs is the ability to re-infect the same host and conferring short term immunity in contrast to other pox viruses and also produce localized skin lesions in humans who are in contact with infected animals and their products. Unique and specific virulence genes located at termini o...
Saman Fatima* and Shazia Iram
...f physical appearance of lesions on peal and the disease severity was then identified. Both classical and molecular method was used for identification of pathogens. Under classical approach the infected sections from the skin of kinnow was utilized to grow fungal colonies on generalized media, i.e. potato dextrose agar, from which six fungal colonies were also isolated and studied further. Under molecular approach direct DNA was isolated from the infected skin...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...owed the signs of ocular lesions associated with plaque formation on the cornea and sclera and papilloma formation on the lateral limbus of eye. The tumors were large and usually protruded through the palpebral fissure and infiltrate the entire eye ball. Diagnosis was made based upon the clinical and histopathological examination of the tumor sample. In all cases, tumor resection was performed followed by cauterization of the remaining surface with silver nitr...
Khadim Hussain Wagan1*, Muhammad Ibrahim Khaskheli2, Jamal-U-Ddin Hajano1 and Abdul Ghani Lanjar2
...arly, length of necrotic lesions was also significantly higher in MPS16 (6.67 cm) followed by MPS17 (6.33 cm), MPS12 (6.30 cm), MPS15 (6.17 cm) and MPS 13 (6.07 cm). The predicted isolate (MPS16) was aligned with different species and observed that MPS16 belongs to M. phaseolina isolate. The sequence showed high identity with sequences with Macrophomina sp. from the NCBI database (100%); In contrast, alignments among the same genera and species demonstrated 91...
Attaur Rahman1, Sadeeq ur Rahman1,*, Ashfaq Ahmad2, Abdul Sajid1, Muhammad Asif Hussain1, Mian Muhammad Salman1, Niamat Ullah Khan1, Kamran Khan3, Fazli Rabbani1, Muhammad Akbar Shahid4, Irshad Ahmad5 and Shakoor Ahmad6
...ly. Interestingly, gross lesions were found similar comprising hydropericardium, hepatomegaly, hepatitis, nephritis and spleenomegaly. Altogether, albeit, FAdV-4 caused similar symptoms and lesions in ostriche, however, mortality remained comparatively lower....
Rana Waqas Arshad1, Asim Aslam1, Muhammad Saeed Imran1, Kamran Ashraf2 and Raheela Akhtar1,*
...ently, histopathological lesions of IBD were less evident in live vector vaccine group in comparison to other groups. In addition live vector vaccine improved the feed conversion ratio (FCR) by keeping the bird healthy and by decreasing the immunosuppression. These results indicated that live vector vaccine has overall positive impact in terms of immunity, histopathology of bursa of Fabricius and FCR. These results can be implemented in field for complete prot...
Saeed Murtaza1,*, Nazir Ahmad2, Muhammad Asif Raza3, Muhammad Saleem Akhtar1, Muhammad Mazhar Ayaz4, Muhammad Ali5 and Rais Ahmed6
...d bucks with spontaneous lesions of testes and epididymides and 10 normal organs was carried out, using a B-mode ultrasound scanner. Each organ was incised and the nature of lesions and their contents was observed. Ultrasonically, testes of normal rams and bucks appeared homogeneous and moderately echogenic. Mediastinum testis was seen as a centrally located hyperechoic line in longitudinal images and by an almost circular h...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...anis antigens in the lesions of all examined tissues. Strong positive staining was primarily found in the spleen, liver, and testicle. In conclusion, this study was the first to report the isolation of two B. canis strains from pet dogs in Sichuan province, southwestern China, and to further evaluate B. canis antigen location in tissues. Our study will contribute to the understanding of B. canis pathogenicity in naturally-infected pet ...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2, Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1, Aneela Zameer Durrani4 and Muhammad Usman4*
...PP. Maximum pathological lesions (50-75% of surface area) were seen in 34.69 percent lung samples, chronic type pleurisy in 97.96 percent, maximum aggregate pathological lesion score in 65.31%, while 48.98% lung samples had 6-10 numbers of sequestra. Besides, infiltration of inflammatory cells, congestion, hemorrhages, necrosis and other degenerative changes were observed in tissue samples of infected cattle. Significantly higher (P<0.05) micro-pathological...
Muhammad Anwarullah1*, Aneela Zameer Durrani1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Ghulam Mustafa3, Muhammad Husnain1 and Muhammad Usman1
...group. Histopathological lesions were observed before and after the therapeutic trials and lesions scoring was given according to the standard. On the basis of the changes in histopathological lesions, the effect of these therapeutic agents was noted. Amikacin showed marked differences in terms of decrease in severity of lesions weather used alone or in ...
Muhammad Usman Ghazanfar1, Waqas Raza1*, Waqas Wakil2,3, Imtiaz Hussain4 and Misbah Iqbal Qamar1
...ion. Numbers and size of lesions per plant was reduced by application of SA and BABA. Plants treated with BABA exhibited increased resistance against herbivory by aphids (Myzus persicae Sulzer) (Homoptera: Aphidiae), thrips (Thrips tabaci Lindeman) (Thysanoptera: Thripidae) and whitefly (Bemisia tabaci Gennadius) (Hemiptera: Aleyrodidae) compared to SA treated plants. Potato plants treated with SA and BABA had higher peroxidase and polyphenol oxidase activity ...
Saira Saleemi1*, Zafar Iqbal1, Abdul Nasir Khalid2
... gills, damaged fins and lesions all over the body. The fish specimens showed mild to severe infection. The infection caused by fungus was confirmed by isolating the pathogenic fungus from affected skin, gills, fins, heart, liver, kidney and intestine of fish and culturing it on four different culture media; Malt extract agar (MEA), Corn meal agar (CMA), Sabouraud dextrose agar (SDA) and Potato dextrose agar (PDA). The inoculated culture plates were incubated ...
Muhammad Riaz1*, Naureen Akhtar1, Salik Nawaz Khan1, Muhammad Shakeel1,2 and Ateeq Tahir1
Neocosmospora rubicola: An Unrecorded Pathogen from Pakistan Causing Potato Stem Rot
...lants were necrotic stem lesions near the collar region. Causal organism was isolated from the infected tissues, purified and identified on the basis of morphological characters, nucleotide sequences of internal transcribed spacer region (ITS) and partial beta tubulin gene. Phylogenetic analysis was also conducted to determine the phylogenetic relationship of this species with other reported species of this genus. Pathogenic potential of the isolate was verifi...
Mohammad Enamul Hoque Kayesh1,2*, Mohammad Tufazzal Hussan3, Md. Abul Hashem2,4, Mohammad Eliyas5 and A.K.M. Mostafa Anower1
Lumpy Skin Disease Virus Infection: An Emerging Threat to Cattle Health in Bangladesh
...he appearance of nodular lesions in the skin covering all parts of the body. LSD is currently endemic in many African countries and causing significant economic losses. LSD has recently spread out of Africa into the Middle East region, and still continuing to spread other territories of the world. In September 15, 2019 the first occurrence of LSD outbreak in Bangladesh has been reported to the OIE (World Organization for Animal Health). LSD is a vector-borne d...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
...ls (50%). Major necropsy lesions observed were pin point hemorrhages in proventriculus, demarcated button-like ulcers of intestinal mucosa, hemorrhages in trachea and spleen. Histopathological lesions observed in proventriculus were erosions in epithelium, localized hemorrhages and congestion in the glandular region. In small intestine of affected birds, villi appeared hyperemic and ulcerated. Tracheal surface epithelium oft...
Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*
...;">Atrichia with papular lesions (APL) is a rare autosomal recessive form of total alopecia, characterized by hair loss soon after birth and the development of papular lesions of keratin-filled cysts over extensive areas of the body. Two consanguineous families were enrolled from Basti Mochi Wala, Mouza Gulab Shah in Muzaffargarh District of Punjab (Pakistan) having multiple siblings suffering from alopecia. The aim of this ...
Hagar E. Mohammed1, Ranwa A. Elrayess2 and Heba N. Gad El-Hak2*
...testes histopathological lesions and histochemical alteration associated with a reduction in epididymis weight, abnormality in the sperm indices and a decline in serum testosterone levels which may have a bad impact on male infertility, on the other side, treatment the rats with the combined extract of different doses of A. monosperma or M. piperita attenuated the deleterious damage to the testes, the sperm index and the sex hormones. This study ...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2,
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
...ss and histopathological lesions a total of 50 animals were necropsied infected with C. perfringens type D. A total of 43 (86%) sheep were recorded having hemorrhages on serosa of intestine followed by gas filled small intestine in 41 sheep (82%), lesions in ileum and colon in 39 sheep (78%), soft kidney 27 (54%), dark congested liver 26 (52%), nephritis 23 (46%) and spleenitis 13 (26%). Microscopic
Sajid Ali1, Muhammad Avais1*, Aneela Zameer Durrani1, Kamran Ashraf2, Saira Jabeen1, Sajid Hameed3, Muhammad Awais1, Jawaria Ali Khan1 and Imtiaz Ahmad4
... Among the hoof and claw lesions, sole ulcer was significantly (P <0.05) more prevalent than white line disease, sole hemorrhage or inter-digital dermatitis. It was concluded that lameness and hoof lesions are important health problems in studied commercial dairy herds. The occurrence of lameness could be reduced in dairy herds if producers become aware of the associated risk factors, and improve management practic...
El-Habbaa, A.S.
...t. Forty samples of skin lesions from suspected 30 sheep
and 10 goats from different areas of Qaliubeya governorate were subjected
tovirusisolation on embryonated chicken eggs (ECE), antigen detection using agar gel
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were showed in 36 of
samples (28/30 of sheep samp...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...e clinical samples (foot lesions). RNA extracted from clinical samples was subjected to reverse transcriptase polymerase chain reaction( Rt. PCR) nucleotide of FMDV ID and 3D genes were determined using standard automated sequencing technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sharkia/2013/ FMD / (KJ210080) revealed (99–100%) identity with Egypt 2012/ and Palestinian-Gaza virus. Phylogenetic analysis of serotype O of this study from Ismailia...
Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5
...h or without fever. Skin lesions were collected from clinically diseased calves for virus isolation in embryonated chicken eggs for three passages followed by ten successive passages in Madin Darby Bovine Kidney (MDBK) and identification of isolated virus was done by indirect immunofluorescence test. The serum antibody titers were determined by serum neutralization test (SNT) and Enzyme linked immunosorbant assay (ELISA) in calves and also their dams. The resu...
K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2
...ere gave chlorotic local lesions. Eleven potato cultivars
(Solanum tuberosum) mechanically inoculated with PVY isolate under greenhouse condition
were divided to three categories, resistant (Diamond, Execusa, Hermes and Valor), tolerant
(Sisi, Lady Balfor, Nikola, Ditta and Lady Rosetta), susceptible (Anabel and Spunta) based on
different external symptoms, the severity infection and virus concentration. Expresse...
Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*
... the characteristic pock lesions for each strain with titers reached 4.5 and 3.5 log10 EID50/ml at 5th passage for FPV and PPV isolates, respectively. The isolated strains were further identified using the specific hyper-immune sera for each virus by virus neutrization test (VNT). As the Neutrization index (NI) for PPV and FPV isolates were 2 and 2.75 when neutrized by FPV hyperimmune sera (HIS) while it was 2.5 and 2 when neutrized by PPV (HIS). DNA was extra...
Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed
... for any cases with skin lesions as early as possible to diagnosis and apply adequate control measures. The results encountered in the present study revealed that cattle infected with LSD exposed to strong oxidative stress so recommended to use antioxidants in infected animals during treatment....
Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5
...11 day which showed pock lesions and CPE on MDBK cell culture. Isolated virus was identified by indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against Lumpy Skin Disease Virus. Further identifications were carried out by polymerase chain reaction (PCR). Results: 15 samples were +ve for LSDV by conventional PCR.The results showed that 11/23 biopsies were positive by IFAT. Molecular identification of LSD virus by using RT-PCR, r...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
...ethods: Thirty five skin lesions of nodular and crusted scab samples were collected from clinically
infected SPV sheep and were prepared and isolated on chorioallantoic membrane of 9-11 days old
emberyonted chicken egg for three passages. The isolated samples were identified by EM and PCR.
Results: Five samples were positive and showed a typical pock lesion., the isolated strain was
identified as a sheep pox viru...
Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3
...produced prominent local lesions. The virus isolate was then propagated in
potato Ditta cv. plants, The virus was identified on the bases of host range, symptomatology,
transmission and serological diagnosis, in addition to the ultrastructural changes produced in potato
leaf cells infected with AMV .
Results: Reaction of thirteen plant species and cultivars belonging to four families (Amaranthaceae,
S...
Shimaa M. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A. Eid
...d postmortem
lesions that almost associated with ARV infections. Further molecular detection of ARV within the 18
clinically affected samples (n=18) by RT-PCR using a specific primer set targeting a conserved
sequence within ARV- sigma C protein revealed 7/18 positive samples. All positive samples (7/18)
were successfully isolated on specific pathogen free embryonated chicken eggs (SPF-ECE). Additional
Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2
...ting cattle causing skin lesions. It`s a member of Poxviridea family, genus Capri-pox,
and closely related to sheep and goat pox. In Egypt, the protection from lumpy skin disease (LSD)
among cattle population was carried out using sheep poxvirus vaccine. Last year (2016-2017) revealed
many cases of LSD in sheep pox vaccinated cattle in Egypt.
Objective: In this study LSDV was isolated from sheep pox vaccinated ca...
Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1
...n to observe microscopic lesions and to detect CAV
antigens by immunohistochemically in thymus and bone marrow tissues of SPF chicks infected with
CAV.
Methods: In this study, 30 layer farms of age 40 to 100-days-old from different governorates
(Alexandria, Gharbia, Giza and Qalubia) were examined for CAV with real-time PCR and
histopathological examination. The positive samples were prepared and inoc...
Samira M. Bolis1, Magda Mahmoud2, Salah E. Gumma3, Naser E. Bilal1 and
Isam ElKhidir4
...t is symptomless and its lesions can regress by time. On the other hand there
is no cell culture for Human papilloma viruses (HPVs).
Objective: Detection of HPV genotypes assocciated with FEH in childern aged three to fourteen years
old.
Methods: Fourty seven biopsies and oral smears were collected from Children (3 -14) years old, who
attended the pediatric dental clinics of Faculty of Dentistry, Univ...
Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2
...rated IBD characteristic lesions.
Objective: In present study the aim was to molecular characterize IBDV field strains detected in flocks
located in 4 governorates. Also, a phylogenetic analysis based on the sequence of the hyper variable
region of VP2 gene was carried out.
Methods: Forty field bursa samples were collected from different localities. IBDV was detected using
RT-PCR. Sequence analysis of...
Samah A. Mokbel, Eman A. Ahmed, Hanan F. El-Kammar, Ahmed A. Kheder
...and isolation from local lesions on
Chenopodium amaranticolor as a local lesion host. Eleven plant species belonging to four families
were used to confirm the presence of CMV in the inoculum. Detection of the coat protein gene of
CMV in infected leaves has been done by reverse transcriptase-polymerase chain reaction (RT-PCR),
and the appearance of 678 bp bands confirmed the expected size of such gene. Light and t...
Yonis Abukar Mohamed1, Md. Nazrul Islam1, Md. Haydar Ali1, Mahfuza Akther1 and Abdiaziz Idiris Mohamud2*
...ghout the capsule. Gross lesions of fascioliasis found in the liver were increase in the size of the organ were 14.6%. The infected bile ducts were also filled with blackish brown exudates giving the pipe stem appearance of the liver was noticed 13.3%. In histopathological study of acute Fascioliasis, there was lot of hepatocytic alterations like swelling of individual hepatocytes by increase in size and characterized by opaque cytoplasm. Sometime there was fa...
Yonis Abukar Mohamed1, Shafii Abdullahi Mohamed1,2, Abdiaziz Idiris Mohamud1,3*, Abdiaziz Ahmed Mohamud1,3, Kassim Abdullahi Jimale1,2 and Said Ali Ibrahim2
...d or active wounds (skin lesions), 17.4% scars, and 28.0% both skin lesions and scars. Moreover, dermatological diseases were also encountered, habronemiasis 59.1%, ectoparasite 35.7%, Sarcoid 5.1%. Results that were obtained from the indirect assessment of donkeys’ welfare indicated that most donkey owners in the region have little or no knowledge and information on donkey’s welfare matters. Limitation of taking...
Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4*
...the association of udder lesions with Subclinical Mastitis (SCM) in dairy cows of Chattogram Metropolitan Area (CMA) and Patiya Upazila of Chattogram district during the period from December 2017 to May 2018. For this, 264 milk samples and 155 swab samples were collected from 50 different dairy farms available in CMA and Patiya Upazila. A pretested questionnaire was implemented during sample collection for identifying the associated risk factors of udder
Jiao Ma*
...f the treatment of heart lesions. The work of cytokines and growth factors has been found to play an important role in increasing endogenous cardiac stem cell proliferation and migration capacity. One of the successful approaches is to use these factors along with cardiac stem cells to boost the repair efficiency of heart injury. Therefore, in the present review study, the types of cardiac stem cells and their molecular phenotype, cardiac stem cell genetics an...
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...ltrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenoty...
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...ltrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenoty...
Fujun Miao1, Chunlan Shan2, Wei Yang3, Hao Wang3, Shuxiang Geng1 and Delu Ning1*
...antly decreased, and the lesions of ethanol-induced liver injury were relieved compared with the ethanol (ETH) group. Walnut oil significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the liver of ALD. Walnut oil exerted the anti-inflammatory effects in the liver of ALD by decreasing the liver index, malondialdehyde (MDA) contents, and the expression levels of F4/80. The expression levels of key factors in t...
Kanakuntla Sandhyarani*, Dhoppalapudi Madhuri, Yadala Ravikumar
...t, Poultry, Pathological lesions, Treatment ...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...cal signs, mortality and lesions of IBD in this study. The collected bursae showed pathological changes related to those of IBD infection. Furthermore, the isolates were molecularly characterized using reverse transcription polymerase chain reaction (RT-PCR) technique and gene sequencing of partial portion of VP2 gene. The two isolates (Genbank accession numbers MW925051 and MW9250522) were characterized as very virulent IBDV (vvIBDV). The obtained nucleotide ...
Barirah Rehman Talpur1, Zaheer Ahmed Nizamani1*, Imdad Hussain Leghari2, Mansoor Tariq1, Aisha Rehman3, Shahnawaz Kumbhar1
...While in broilers, toxic lesions were observed on mucus membranes of duodenum and proventriculus along with nephritis. Histological lesions observed in livers of both rabbits and broilers included dilation of central vein and sinusoids and fatty degeneration of hepatocytes. Kidney tissues of both rabbits and broilers revealed marked shrinkage of glomeruli with widened bowman’s spaces along with inflammatory cellular in...
Hany M.R. Elsherif1, Ahmed Orabi2, Hussein M.A. Hassan3, Ahmed Samy3*
...cored zero in intestinal lesions scale. The addition of SF or SP were significantly enhanced (P<0.05) the lactobacillus count when compared to the control, while the quantity of Clostridium perfringens intestinal colonization was not exist. When OAS’s were introduced to broiler diets, total protein, globulin, albumin/globulin, T3, T4, and total antioxidant capacity levels were dramatically increased, while alanine aminotransferase (ALT), aspartate ami...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
... No characteristic gross lesions were observed except heamorrhages in brain, congested liver, spleen, brain and muscles besides greenish content of the gizzard. The histopathological examination of lung, brain, liver, and trachea of these diseased quails revealed non-suppurative encephalitis, perivascular cuffing, congestion of hepatic sinusoids, degenerative changes in the hepatic cells and interstitial lymphocytic aggregations in the liver and focal pneumoni...
Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*
...58.3%). The frequency of lesions and E. coli densities in the heart, liver, and spleen of chickens treated with MHH6 and PR2 phages were greatly decreased in infected chickens. After 98 days, the body weight of birds from the E. coli-infected groups treated with MHH6 and PR2 phages was lower than individuals in non-E. coli-infected groups (1,313-1,324 g/bird) but higher and significantly different from those in the PC group (1,172 g/bird). The majority of comm...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
...passage and typical pock lesions appeared at 3rd passage. Indirect immunofluorescent technique (IFT) was used for confirmation of BoHV-1 presence in CAM; the CAM with clear pock lesions showed yellowish-green fluorescence under the fluorescent microscope. The phylogenetic tree constructed from partial sequencing of gB of our isolate was divided into two clades; clade 1 BoHV-1 (BoHV-1.1 and BoHV-1.2) clade 2 (BoHV-5). Our iso...
Tariq Mehmood1, Muhammad Arif Khan1, Ayesha Safder Chaudhry1, Kamran Ashraf2, Muhammad Mohsin Ali1,3, Muhammad Faisal Ayoob3*, Mudassir Hussain4, Farrukh Saleem3 and Muhammad Fayaz3
...verse local injuries and lesions are corrected by resection of the intestine. Twelve cats were subjected to 3 variable degrees of jejunal resection, i.e. group A (20%) and group B (40%), while group C (0%). Sampling was performed on days 0, 15, and 30 to test various clinical and biochemical parameters. Results showed weight loss in all groups until day 15 post-surgery while in group A and C gradual weight gain was noted. Fecal consistency was observed in all ...
Veronica Ngozi Emenuga1, Clara Idara Eleazar2*, Seto Tunrayo Aladenika1
...ilk. Scrapings of fungal lesions from 302 cattle were examined alongside with samples of milk and sawdust beddings. Culture, identification and characterization of isolates were carried out using standard methods. Antifungal biogram was performed. Result showed that all the cattle and the bedding tested positive for fungi, while 41(82%) of the milk samples had fungal contamination. Rate of isolation from cattle, bedding and the milk was not significantly (P=...
Muhammad Kashif Iqbal1*, Khalid Mehmood2,4, Shakeel Ahmed3, Fazul Nabi4, Muhammad Arif Rizwan1, Muhammad Kaleem1, Mushtaq Ahmed1, Abdul Waheed1 and Jiakui Li5
...scular growth plate with lesions in proximal tibiotarsal bone. The aim of present study was to investigate the antioxidant capacity of FK228 and role of vascular endothelial growth factor (VEGF) and Flk-1 genes in thiram induced TD. One hundred and fifty broiler chicks were equally divided into 3 groups: control; thiram fed; and FK228 treatment group. Expressions of VEGF and Flk-1 genes were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR...
Muhammad Haris Raza Farhan1*, Qamar Iqbal1, Tariq Jamil1, Muhammad Fayaz2, Abdul Kabir2,3, Eid Nawaz2, Marjan Ali2, Shumaila Manzoor2
...f some animals. Pox-like lesions were also found on the skin of some goats.
Keywords | Parasitic infections, Babesia, Malnutrition, Worm infestation, Stress
...
Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1
...ee from any pathological lesions in the negative animals. Immunohistochemical, BVDV antigen was detected in ear notch, ovaries, fallopian tubes and uterus of one cow (1/30) (PI animal). Whereas, BVDV antigen was detected only in the genital organs of acutely infected animals (2/30) but ear notch was negative. The positive BVDV antigen was clearly observed in the PI cow in the ovaries (primordial, growing, follicles, follicular fluid, granulosa cells, ovarian s...
Caiyun Ma1, Hebao Wen1, Lu Li2, Gaofeng Liu1, Chunjing Wang1, Yu Guo1, Shiji Fang1, Hongyu Wang1* and Changqing Liu1*
...t of articular cartilage lesions remains an important challenge. Articular cartilage, the target of osteoarthritis (OA), contains renewable cartilage stem/progenitor cells (CSPCs) to maintain the tissue homeostasis. Compared with other adult stem cells, the self-renewal capacity and preference for chondrogenesis of resident CSPCs have propelled their exciting therapeutic prospects for cartilage repair. Tibetan mastiff, the ancestor of large breed dogs worldwid...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed Elbayoumi1 , Sameh Abdel- Moez Ahmed Amer1, Asmaa Mahmoud Matoaq1 , Mohamed Abdel Aziz Kutkat1, Gomaa Abd El-Rhim Abdel-Alim2
...signs, post-mortem gross lesions, growth performance as well as mortalities are all recorded and evaluated. All vaccination protocols were able to induce antibody levels for NDV after vaccination with varying titers. Overall, the serological response at challenge day were significantly with higher titers in groups vaccinated with inactivated NDV genotype VII vaccines when compared with other groups. Regarding broiler performance parameters it was noticed that,...
Ahmed H. Massoud1, Mohamed S. Ahmed2, Moustafa Saad-Allah1, Aly S. Derbalah1, Ashraf Albrakati3* and Ehab Kotb Elmahallawy4*
...he obtained histological lesions were correlated with the increased level of biochemical enzymes in target organs. Additionally, hepatotoxicity and nephrotoxicity were obvious in rats treated with cymoxanil, meanwhile, neuronal and pulmonary changes were nearly the same in all doses of the used pesticides. Taken together, our study recommends examining pesticides for their possible adverse effects on animals and humans in case of repeated use, even in small do...
Muhammad Izhar ul Haque1, Farhan Anwar Khan1*, Umar Sadique1, Hamayun Khan1, Zia ur Rehman1, Salman Khan1, Hayatullah Khan1,2, Faisal Ahmad1,3, Mumtazur Rahman1, Faiz Ur Rehman1, Muhammad Saeed1, Mehboob Ali1 and Saqib Nawaz1
...ciated histopathological lesions and PCR in sheep (Ovis aries) and goats (Capra aegagru hircus) in district Peshawar. Serum and fecal samples were collected at random both from commercial farms and abattoirs. Additionally, tissue samples (intestine and mesenteric lymph node (MLN)) were collected at random from sheep and goats at abattoirs of district Peshawar. Analyses of serum samples by iELISA revealed the presence of antibodies against MAP in 9% sheep and 5...
Maria Al Mazed1*, Md. Ashikur Rahman1 and Sk. Istiaque Ahmed2
...e retardation of growth, lesions in liver and damages in kidney. They are also causing infertility in animals. Chronic exposure and excessive concentrations are also deleterious for the normal physiological functions of human. Consumption of fishes contaminated with toxic metals are neurotoxic and carcinogenic to blood, lungs, kidneys, bones, liver and other vital organs of human. The present review outlines the contamination of aquatic environment with heavy ...
S. Feroza1, A. G. Arijo1†, F. M. Bilqees2 and M. S. Phulan1
...ealed histo-pathological lesions of infected liver that showed traumatic and toxic effects produced by the migrating A. galli larvae. Except on the top, central veins were not obvious. Inflammatory tracts produced by migrating larvae were prominent. Large vein with portal sluggish consisted of fibrinous material and inflammatory cells, disarrangement of hepatici and shrinkage of hepatocytes were found to have resulted into dilation of sinusoids....
Öznur Özil*, Öznur Diler, Mevlüt Nazıroğlu, Aşkın Atabay
... fibrotic muscle tissues lesions in Garra rufa.
Keywords | Clinostomum complanatum, Garra rufa, Histopathology, Prevalance, Intensity
...
S. Feroza 1, A. G. Arijo1†, F. M. Bilqees2* and M. S. Phulan1
...ealed gross pathological lesions of infected organs viz., small intestine, liver, lungs and heart. Hemorrhagic enteritis and acute fibrinous enteritis with clotted blood, hemorrhagic and reddish spots on intestinal wall, inflammation and necrotic patches with consolidation of intestinal contents were generally observed. Infected liver showed dark red coloration. Severe congestion, chronic pneumonia, exuded in one lobe of the lung were seen, whereas, necrosis a...
Lulu Ding, Ke Wang, Ruxue Huang, Wenjing Yu, Bingzhao Yan, Mengli Jiang and Jicang Wang*
...ted, and a large area of lesions occurred. However, treatment with Nar slowed down the occurrence of oxidative stress induced by Cd. Therefore, as an effective antioxidant, Nar has a certain protective effect on oxidative stress and kidney injury induced by Cd....
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...s (73.9%) showed visible lesions on postmortem examination while the remaining 18 animals (26.1%) showed no visible lesions. Using bacteriological examination of tissue specimens, 48 out of the 69 positive tuberculin reactors (69.6%) revealed successful M. bovis isolation. Using ELISA on serum samples collected prior to skin testing from the positive tuberculin reactors, only 26 cases (37.7%) were positive. The IQRT Anigen l...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed El-Bayoumi1, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila1, Gomaa Abd El-Rhim Abdel Alim2
...th IBD and NDV infection lesions were found in dead birds of all challenged groups. Chicken groups challenged by IBDV or NDV after treatment with Lector showed milder histopathological changes than Orego solution and non-treated groups showed the most sever lesions. Chicken groups challenged with both virulent IBDV and NDV either treatment with Lector® or Orego® solution or non-treated showed no marked difference in ...
Hussein Jabar Jasim1*, Amer Murhum Al-Amery2
... the most frequent gross lesions. Meanwhile, the histopathological examination showed hyperplasia, fibrous proliferation in the portal area and coagulative necrosis of hepatocytes with large numbers of inflammatory cell infiltration such as eosinophil, Kupffer cell and scattered lymphocytes in the area previously migrated by young flukes as well hepatocytes displayed a fatty change with dilated hepatic sinusoids. Furthermore, our findings show that the bile du...
Esam A Razin1, Hassan Sobhy2, Tarek R. AboElnaga1, Asmaa A. Darwish1*, Rasha S. Mohammed1
...ination clarified severe lesions similar to TG. While, BG clinicopathological parameters and histopathological results were so close to CG. CK, CK-MB, LDH, and GR had high sensitivity and NPV values and moderated specificity, PPV, LR, and accuracy rate values. Conclusion: Basil plant extract is an efficient anti-trypanosomal drug. CK, CK-MB, LDH, and GR are moderate biomarkers for disease diagnosis and its treatment monitoring.
Abdirahman Barre*, Karanja D Njuguna, Bebora Lilly Caroline and George Chege Gitao
...testing and pathological lesions that encountered at post mortem inspection of camel meat. Three sub-counties; Garissa Township, Dadaab and Balambale were purposefully recruited based on presence of camel slaughterhouses and accessibility. A hundred and sixty (160) camels were selected from 238 presented during the visits based on clinical manifestations suggestive of Brucellosis obtained upon ante-mortem examination and clinical history from owners. Sero-prev...
JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5
A. S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik2; Nashwa M. A. Abd El Mohsen1; and H. M. Abdelmaksoud1
...osts as it induced local lesions and top necrosis on Capsicum annuum L. cvs. California Wonder & Godion as well as Lycopersicon esculentum Mill. cvs. Streen B and Casel Rock. Solanum tuherosum L cv. Cara. Nicotiana glutinosa L. Nicotiana benthamiana gave systemic infection only. On the other hand. systemic symptomless infection had occurred with N. tabacum L. cvs. White Burley and Xanthine. Whereas PAMV didn't infect Ch. umaranticolor Cost. Reyn and D. met...
Sen Ye1, Jiangang Pang2 and Xiuli Wang3*
...y in ischemic peripheral lesions. Warfarin pretreatment enhanced the expression of PAR-1 and PAR-2 in preischemic and post-ischemic lesions, whereas rivaroxaban pretreatment had no such effect. This study suggests that rivaroxaban pretreatment causes lower risk of cerebral hemorrhage in mice than warfarin pretreatment. we conclude that, compared with warfarin pretreatment, the relative down-regulation of PAR-1 and PAR-...
Munir Ahmad1, Abdul Ghaffar1, Riaz Hussain2* and Rifat Ullah Khan3*
...ferent histopathological lesions in liver of treated test specimens including edema, hemorrhages, atrophy of hepatocyte and hepatocyte having eccentric nuclei. In the brain, different histological alterations like necrosis and degeneration of neurons, and microgliosis were seen, whereas the heart of treated fish showed hemorrhages, edema, and neutrophilic myocarditis. In conclusion, Pymetrozine appears to have harmful effects on blood biochemistry and histolog...
Falah Mahmood Hameed1*, Asaad Khalaf Talal AL-Shuwaili2, Eman Jawad Jabber3, Mayada Sahib Hassan1
...full-thickness cutaneous lesions, 24 clinically healthy adult male rabbits weighing 1.5 to 2 kg were utilized in the experiment. The interplay between intramuscular of 5 mg/kg xylazine hydrochloride, 35 mg/kg ketamine hydrochloride, and 1 mg/kg diazepam were carried out. Based on the sort of treatment applied, these animals were allocated into three groups (A, B, and C). Chitosan powder was applied locally to wounds in group (A), as well as administered orall...
Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin
...ocation, and gross tumor lesions were documented, and positive ovaries were collected for further histological and immunohistochemical examination.Results: Interestingly, our results diagnosed 15 cases of sex cord-stromal tumors (8.30 %) in the ovary of dromedary camels included; adult granulosa cell tumor (0.55 %), interstitial cell tumor (0.55 %), thecoma or fibrothecoma (1.10 %), steroid cell tumor-NOS (0.55 %), and granulosa-theca cell tumor (5.55 %). Spec...
Skenndri Safae1*, Charrat Nadia2, Jmiai Mehdi3, Nassik Saadia1
...tection. The microscopic lesions found on most of the farms of the P group on day 35 indicated the ongoing of a regenerative process, contrarily to the farms of the D and G groups, in which the guts still underwent inflammation on day 35. Coccidiosis infection was heavily widespread on birds at day 35. Differences between the farm in D and P groups weren’t striking as the disease was detected on the intestinal portions of almost all the broilers of the f...
Walaa I. Mohameden1, Haidy G. Abdel-Rahman2, Ibrahim M. Hegab3*
...lves suffering from skin lesions were enrolled in this study and the diet was supplemented with mineral mixture for 60 days. Skin conditions and behavioral modifications were monitored alongside blood and plasma samples were harvested at days 0, 30, and 60 of the study for hematological and biochemical analysis. Results revealed an overall improvement in animal health, skin conditions, and liver and kidney functions. Significant (P < 0.05) increases in plas...
Mohamed Mahrous Amer1*, Hanaa Sayed Fedawy2, Hoda Mohamed Mekky2, Khaled Mohamed Elbayoumi2, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila2
...in thickness, postmortem lesions in the 3rd day post infection with s.c. yellowish suppurative exudates, pericarditis and perihepatitis were prominent E. coli infected with hepatic subcapsular hemorrhage mostly in S. aureus groups. Hematological parameters were mostly affected in all infected non-treated groups compared to negative control without significant difference. Histopathological changes of infected non-treated groups showed inflammation of s.c tissue...
Muhammad Nasir Bhaya1,2* and Hikmet Keles2
...decrease of pathological lesions was found in CP+EA groups especially in the CP+EA75 group. In immunohistochemical sections, 8-hydroxy-2-deoxyguanosine (8-OHdG) and hypoxia-inducible factor-1 alpha (HIF-1α) revealed less positivity in EA received groups but B-cell lymphoma-2 (Bcl-2) revealed more positivity as compared to CP. Both histopathological and immunohistochemical findings revealed that EA has a protective effect against CP-induced renal damage i...
Irtaza Hussain1, Muti ur Rehman Khan1*, Asim Aslam1, Masood Rabbani2 and Ahsan Anjum1
...all feeding, presence of lesions on the lips, and injuries on the skin caused by prickly trees and cotton stubbles were found significantly (p<0.05) associated factors in goats; and age and injuries on the skin were important associated risk factors in sheep. The maximum occurrence of contagious ecthyma infection was found from September to December in both goats and sheep; having a significant association in goats (χ2=39.2) (P<0.0001). Likewise, the...
Peter Amuguni Mbato*, Wanzila Usyu Mutinda, Noah Kipkoech Maritim, Nicholas Edwin Odongo
...redisposing them to skin lesions from moist litter. This study aimed to determine whether the free-range production system (FRPS) for broiler birds would reduce foot pad lesions and the risk of bacterial infection. The study followed a completely randomized design, comprising of four treatments and three replications, with each replication consisting of 20 birds. Treatments A, B and C, where the birds were under FRPS at 2, 3...
Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2
...tions, gross post-mortem lesions, Humoral immune response and also quantification of virus shed post-challenge (PC) via real-time QRT-PCR were all screened and precisely recorded. The results revealed that, group A chickens developed the highest humoral antibody titers throughout the vaccination schedule and conferred a complete protection against mortality with milder clinical signs, moreover displayed a significant decrease in the shedding of NDV with drasti...
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
...rtality rates and bursal lesions in al-El-Fayoum province, Egypt during 2019 and 2020. Reverse transcription PCR was used to detect IBDV in clinical samples by amplification of the hyper variable region (HVR) of VP2 gene. Genetic analysis of the PCR amplicons has revealed that 3 samples belonged to very virulent IBDV (vvIBDV). The genotyping of Egyptian vvIBDv indicate progressive evolution compared with previously isolated strains which indicates persistence ...
Ahmed Jaafar Mousa*, Nothaila Rasheed Hamid
... of this illness include lesions in the upper gastrointestinal tract. The present study’s goal would be to study the prevalence and genetic characteristics of T. gallinae in racing pigeons in Thi-Qar province, Iraq, utilizing the ITS1-5.8s rRNA-ITS2 gene. 100 samples were collected from racing pigeons between September and December 2022. The swabs were taken from the throat, pharynx, crop, and esophagus with a cotton swab. Utilizing direct smear and poly...
Md. Ariful Alam1, Mst. Misrat Masuma Parvez1, Md. Salauddin2*, Rakibul Islam1 and Md. Bazlar Rashid1
...ps showed fewer abnormal lesions whereas T3 showed significantly better lesions than others. Yeast showed good anticoccidial effects and can be used to explore novel therapy against coccidiosis....
Md. Ariful Alam1, Mst. Misrat Masuma Parvez1, Md. Salauddin2*, Rakibul Islam1 and Md. Bazlar Rashid1
...ps showed fewer abnormal lesions whereas T3 showed significantly better lesions than others. Yeast showed good anticoccidial effects and can be used to explore novel therapy against coccidiosis....
Wenxiao Jia1*, Yilinuer Yilihamu1, Yunling Wang1, Shuang Ding1, Dilinuerkezi Aihemaiti1, Hanjiaerbieke Kukun1 and Yanhui Ning2
...ine the abdominal aortic lesions. The overall mortality rate of the New Zealand rabbit model of unstable atherosclerotic plaque was 17%. The MRIs indicated that the abdominal aorta in the experimental group was eccentrically thickened, while the vascular wall of the abdominal aorta in the control group exhibited no abnormality. The intima of the abdominal aorta in the experimental group showed pathological manifestations of unstable atherosclerotic plaque, whi...
Abdul Kabir1*, Anees Ur Rahman2, Inzamam Ali Shah2, Ibad ur Rahman2
...is characterized by pock lesions on the skin and mucous membranes of the affected animals and humans, with complications such as mastitis, secondary infections, and reduced milk production. Buffalopox is transmitted through direct contact with infected animals or their secretions, or through fomites. The disease poses a serious threat to the livestock industry and public health, especially in the absence of smallpox vaccination. This review summarizes the curr...
Sara H. Zughayyar*, Amer H. Gyad
...ng 500 mg/kg showed mild lesions and lower inflammatory cell infiltration, closely resembling the histological profile of the loperamide-treated group. Subsequent phytochemical analysis revealed the presence of tannins, carbohydrates, phenols, resins, flavonoids, saponins, alkaloids, coumarins, terpenes, and glycosides, while steroids and proteins were absent. The extract displayed antidiarrheal activity comparable to loperamide, showing a significant reductio...
Sidra Hafeez1, Tayyaba Sanaullah2, Hafsa Naeem3, Mah Noor Hassan4, Muttalib5, Farhana Kausar6, Muhammad Salman Hameed7*, Muhammad Anayat Ullah8, Abdul Samad9, Memoona Bashir10 and Sadaf Shabbir11
...e symptoms of dark brown lesions but causal organism identification through DNA is still unknown. We collected infected leaf from C. pepp from the vicinity of The University of Agriculture, Faisalabad and isolated D. bryoniae on the respective media. Then morphologically identification was observed through fungal growth pattern, growth color, spores shape, spores color, hyphal septation. For the confirmation of Koch’s postulates, the pathogenicity test w...
Rasema Majeed, Alaa Kamil Mahmood*
...lls also showed no clear lesions in the islet cells. whereas fifth group was administered 81.7 mg/kg of chitosan nanoparticles orally for 45 days and showed few destructions and vacuolation in the islet cells, regeneration of the islet cells. Pancreatic DNA damage analysis were assessed the results showed that ginger ethanolic extract, chitosan nanoparticles, and ginger ethanolic extract loaded with Chitosan nanoparticles all significantly reduced DNA damage c...
Asma Sadia Authoy2, Aneek Chanda2, Aparna Datta3, Md Shohel Al Faruk4, Towhida Kamal1*
...and cats exhibiting skin lesions. A cross-sectional study was conducted, utilizing comprehensive questionnaires based on various risk factors such as age, sex, breed, vaccination history, deworming, etc. A total of 174 case sheets of dogs and cats with skin lesions were analyzed. The findings indicate a higher prevalence of ectoparasite infestation among dogs and cats in semi-urban areas, recording rates of 56.92% and 76.92%...
El-Kenawy, A. A. and El-Tholoth, M. S.
...e samples include 7 skin lesions and 16 internal organs (lymph nodes (6), lung (4), kidney (3) and liver (3)). Hyperimmune serum was prepared against reference LSDV (Ismailyia88 strain). Immunofluorescence (IF), immunoperoxidase (IP) techniques and polymerase chain reaction (PCR) were used in our study. Chorioallantoic membranes (CAMs) of embryonated chicken eggs (ECEs) were inoculated with Known previously isolated and identified LSDV of 104 8 EID5d0.1 ml for...
Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.
...fered from oral and foot lesions associated with lameness in addition to fever in some and inappetance in others. Saliva and vesicular lesions were taken on glycerol buffer saline for serotyping of FMD virus by using antigen detection ELISA. The saliva and vesicular lesions of the diseased animals were serotyped as FMD serotype O....
SHARAW11'2*S S. S. A.; AL-HOFUFY2, A. N. and AL-BLOW12, M. H.
...as demonstration of pock lesions and cytopathic effect (CPE) due to CPV were observed while CPV virus antigen was detected and identified by indirect immune-fluorescent assay (IFAT) in Vero cells. A trial for development of a simple and rapid qualitative Real-time polymerase chain reaction (RT-PCR) was applied using primer site belongs to Capripowirus to detect CPV load in prepared tissue samples comparing to inoculated...
Ahmed, AmalA.; Zein, Salwa N. and Khatab, Eman A. H.
...rified from single local lesions formed on Chenopodium amaranticolor. CeMV was identified by its host range, symptom expression, modes of transmission and particle morphology. CeMV able to infect only 20 plant species and varieties from 22 tested by mechanical inoculation. The virus was transmitted in a non-persistent manner using Myzus persicae Sulz. Infected plants were reacted positively only with the specific antiserum for the CeMV using double antib...
Ahmed, Amal A. and Fath-Allah, Mervat M.
...rified from single local lesions which formed on cucumber cotyledon and/or Chenopodium quinoa. Identification of this virus was based on host range, transmissibility, and serological tests. CMV was able to infect only 20 plant species and varieties out of 24 tested plants by either grafting or mechanical inoculation, and PPV was able to infect only 19 plant species and varieties. CMV and PPV can be transmitted by Aphis gossypii and Myzus persicae from cucumber...
Abou zead, A.A., Ali, A.A.H.*and Abd El Hkeem M.M.
...cal signs, , post mortem lesions and histopathological changes were recorded. Serosurvey of 80 serum samples revealed 5 (6.25%) positive for the presence of IBDV precipitating antibodies. 3 out 12 pooled bursae (25%) were positive to the presence of IBDV precipitinogen. Trials for virus isolation on the CAM of chicken embryos revealed the isolation of 3 IBD viruses from diseased quails. Isolated IBDV viruses '.vere examined by electron microscopy. The dis...
* Amal Abou El-Ela, A.
...rified from single local lesions formed on cucumber cotyledon. Identification of this virus was based on host range, properties in crude sap, transmissibility, and serological tests. PNRSV was able to infect only 12 plant species and varieties from 19 by grafting or mechanical inoculation, it has a limited range of experimental hosts. The dilution end-point of infectivity was 10-3, the thermal inactivation point was 540C, and longevity in vitro was of 10-14 h ...
S.Y.M. Mahmoud1 and M. Hashem2
...inducing chlorotic local lesions spreads into the veins. The virus was also transmitted by a soilborne parasitic fungus, Polymyxa betae Keskin as a vector and by seed coating with viruliferous cystosori. the thick-walled resting spores of P. betae. as well as by root vortexing in virus inoculum containing carborundum. The virus is not adsorbed to the exterior of the fungal vector but is internalized. Our results indicated that BNYVV is a widespread in sugar be...
A. A. Kheder1; I. A. M. Ibrahim2; H. M. Mazyadl
...s causes chlorotic local lesions on Chenopodium quinoa Wild. Ch. amaranticolor Cost&Reyn and N. tabacum L. cv. White Burley. It also causes local infection followed by systemic chlorotic leaf spot on Petunia hybrida. Lycopersicon esculentum cv. Castle Rock and Vitis labrusca L. Typical chlorotic spots was expressed on the woody indicator GF305 leaves 30 days after double chip budding. Stability experiments of PRMV showed that the thermal inactivation point...
E.F. Mohamed1 and I.M. Sabra2
...d as the number or local lesions induced by ToMV on Nicotiana sylvestris. This reduction increased gradually by increasing GA and IAA concentrations. ToMV inhibition percentages induced by GA and IAA varied according to the time of treatment (1, 2, 3, and 4 hours). High percentages of inhibition were recorded for in Vitro treatment. ToMV inhibition of pre-inoculation treatment was higher than that of post-inoculation treatment. IAA was more effective than GA.<...
H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3
...clinical signs and gross lesions. The mortality rates in these flocks ranged between 9.4-12.8 % (average 11.1%); 16.4-30 % (average 23.2 %) and 12-23.5 % (average 17.75 %) in commercial broilers. commercial layer replacement pullets and native Baladi varieties, respectively. On the other hand. 30% (9/30) of these outbreaks occurred at 18-21 days of age and the mortality rates ranged between 1.4-4.9% (average 3.15%) in commercial broilers and native Baladi vari...
Rawaa Saladdin Jumaa
...ected pigeons with crust lesions in the eye and nostril cere. The DNA extracted from the collected samples was amplified using conventional PCR by amplification of the P4b gene. The positive samples had been inoculated on chorioallantoic membrane (CAM). Thickening of CAM were seen in the first passage, whereas pock lesion at the second (2nd) and third (3rd) passages. The chicken embryo fibroblast (CEF) cells propagated by the prepared infected CAM have shown t...
Mahmoud Hamouda1*, Ahmed Al-Jazzar1, Fahad Al-Hizab1, Ahmed Azab2, Mohamed Al-Hammadi3
...gically. Several uterine lesions either external or internal were noted in 78/340 animals (22.94%) as follows: internal lesions as endometritis in 33 animals (9.71%), metritis in 11 animals (3.24%), endometrial hyperplasia in 7 animals (2.06%), adenomyosis in 5 animals (1.47%) and fibroma in 3 animals (0.88%). External uteri lesions as perimetrial nodules in 10 animals (2.94 %). Perimetria...
Raza Ali Mangi1,2, Ali Raza Jahejo1,2, Afrasyab Khan1,2, Meng-li Qiao1,2, Muhammad Farhan Qadir1,2, Mazhar Hussain Mangi3, Shi-xiong Yang1,2, Xin-yu Han1,2, Sheng Niu1,2, Ding Zhang1,2, Ying Wang1,2 and Wen-xia Tian1,2*
...er TD score with typical lesions in the proximal growth plate than the rGSTA3 supplementary group. A morphological characteristic of TD was measured by TD index. Chicken erythrocytes constitutively expressed transcripts for HDR and PRRs genes. Moreover, the quantitative real time PCR (qRT-PCR) results showed that HDRs and PPRs expressions were suppressed by thiram, which found upregulated in rGSTA3 protein supplementary group. These findings demonstrated that ...
Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil
...less severe pathological lesions in vaccinated challenged chickens than in non-vaccinated challenged chickens. Quantitation of viral loads following challenge indicated reduction of viral loads shed from birds vaccinated with the attenuated AOAV-1 virus. These preliminary insights collectively underline a new hope for the attenuation of live AOAV-1 genotype VII.1.1 as a vaccine candidate that can be used to control ND outbreaks in Egyptian poultry.
...
Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee
...e dose of 1/10 LD50, the lesions were more severe than the lesions at 1/20 LD50. The characteristic histological finding in the liver is severe and generalized hepatocellular necrosis, which revealed congestion, hemorrhage, and degeneration of hepatic cells. Some sections of the kidneys show vacuolation of mesangial cells in the glomerular tuft. Muscles of treated rats revealed severe histopathological changes under stress f...
Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain
...years. Neuropathological lesions are typically modest, despite significant neurologic symptoms and a deadly prognosis. The Rabies virus uses several strategies to circumvent the host’s defenses. As a major zoonosis, initial treatment and successful preventive and control efforts depend on a clear and prompt diagnosis. For the diagnosis of Rabies, conventional techniques such as Direct Fluorescent Antibody Test (dFAT) and histopathology are still employed...
Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
...though, the demyelinated lesions develop throughout the brain, but more frequently are extensive in white matter. Currently, three different approaches are being utilized to treat MS, where synthetic drugs are the most frequently used but they do not cure the disease. Secondly, the stem cell therapy but this too has limited success in treating MS in humans. The thirds technique involving administering hormones has been found to be most effective method but thi...
Fika Yuliza Purba1,4*, Andi Magfira Satya Apada1, Andi Ariyandy2, Irwan Ismail1,4, Subaedy Yusuf3,4
...the animals: Group A (no lesions), Group B (early stage), Group C (advanced stage), and Group D (recovery stage). The results showed that the white blood cell and granulocyte count in Group B was significantly higher than in Group A, while the lymphocyte and mid-cell count in Group B were higher compared to the other groups. The concentration of hemoglobin and hematocrit in Group A was significantly higher compared to the other groups. In contrast, the mean co...
Hayder S. Rwayyih*, Tahani S.S. Al-Azawi
...ted groups show no clear lesions with normal spindle shape cardiac muscle and intercalated disc with normal aorta and epithelial cells. From the other hand, the ovariectomized non- supplemented rabbits show clear lesions in their heart and aorta. There was clear necrosis of cardiac muscle fibers with fatty change in endocardium or papillary muscles due to estrogen deficiency, irregular myocardial endothelial degeneration and...
