Inamullah Khan, Muhammad Subhan Qureshi, Rajwali Khan, Syed Muhammad Sohail, Ijaz Ahmad, Muhammad Shoaib and Asim Ijaz
...s compared to artificial insemination (78%). In humans overall sero-prevalence was recorded 12.12%, 09.59% and 08.59% by SPAT, i-ELISA and PCR respectively. Highest incidence was found in farm workers (14.5%) followed by butchers (10.5%), veterinary assistant (10%) and abattoir workers (08.33%). High incidence was observed in butchers 20.58%, followed by veterinary assistants 14.28%, farm workers 12.28%, abattoir workers 11.53%, farm owners 10.41% and no posit...
Relationship of PIT-1 Gene Polymorphism with Breeding Parameters and Body Weights of Cows and Calves
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...ach animal: age at first insemination, age at first calving, number of semen portions attributable to successful insemination, length of pregnancy and periods of: insemination service, postpartum resting period, interpregnancy period and intercalving period. The study also determined weight of the cow and weight of the calf at calving (including gender). Statistical calculations were perfo...
Irfan1,*, Arshad Javid7, Muhammad Ashraf2, Athar Mahmud3, Muhammad Altaf4, Syed Makhdoom Hussain5, Muhammad Shahbaz4 and Khalid Javed Iqbal6
...d mating. For artificial insemination, male (n = 3) and female (n = 12) birds were kept separately. Semen of the male birds was collected on weekly basis to record morphological characteristics and to inseminate the female birds. Mean abnormal spermatozoa rate was 14.61±1.61% with most common acrosome defects 39.67±3.80 % followed by defects in mid-piece 29.61±0.24%, head defects 10.15±1.21% and tail defects 20.57±1.97%. Laid...
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...ting (NM) and artificial insemination (AI) in fast feathering Huainan partridge chickens. A total number of 360 fast feathering Huainan partridge hens and 40 roosters were randomly divided into two equal groups. For NM, 4 mating cages were maintained each with 5 roosters and 45 laying hens. The other birds were reared in 3-layer complete ladder cages for AI, for which 180 hens were maintained in 60 cages with 3 hens/cage and 20 roosters in 20 cages. Laying per...
Akshay Sharma*, Mohit Mahajan, Madhumeet Singh and Pravesh Kumar
... 63 days post artificial insemination. Monochorionic diamniotic monozygotic twins were confirmed on the basis of different amniotic sacs and corpus luteum on one ovary. Color Doppler study was done to assess the vascularity and functional activity of corpus luteum present on the left ovary. Ultrasonography was employed at weekly interval to judge the viability of embryo (up to 42 days), fetus (after 42 days) and late embryonic mortality, if any. Sexing of the ...
Osman Ergene1,*, Isfendiyar Darbaz1, Serkan Sayiner2 and Selim Aslan1
...40 days after artificial insemination (AI). Transrectal ultrasonography (TRUS) was performed on all cows on days 30 and 40 after AI. Measurements were carried out on 9 cows whose pregnancy was confirmed and 9 cows whose embryonic mortality (EM) was determined. In PAG-Serum, there was a statistically significant difference (P <0.05, P <0.01) between the measurements on 40th day when EM was detected and on the 28th and 32nd days of pr...
An Empirical Analysis of Livestock Activities of the Model Farm Service Center in Khyber Pakhtunkhwa
Tariq Shah1*, Umar Hayat1, Muhammad Suleman Bacha2 and Muhammad3
... diseases and artificial insemination. However, some of the respondents report long distance, lack of proper facilities and high charges for not visiting the livestock experts. Majority of the respondents both member and non-member respondents have not attended any training on livestock or related program. Few of the respondents have participated in field days, workshop and seminars regarding livestock program. Moreover, results reveal that cows’ milk yi...
Olufisayo Bamidele* and Safiriyu Idowu Ola
... 60 and 120 minutes post insemination. Data recorded were Plasma LH levels, kindling rate, total kit born, total born alive, still born, weight of kits at birth and gestation period (days) and were analyzed using Analysis of variance of the General Linear Model procedure and t-test where appropriate. The plasma LH for the AI groups peaked at 60mins while it peaked at 120mins for the control. Kindling rates were 10%, 10%, 60% and 50% respectively for the groups...
Mustafa Boğa1, Kerim Kürşat Çevik2* and Aykut Burgut3
... breeding, and number of insemination (ratio) on cattle milk yield (last seven days in average). For this purpose, the milk yields of 156 Holstein Friesian cattle were investigated according to different age, lactation, calving and insemination associated parameters. Optimum values in literature were organized by an expert in establishing classification data. The expert determined the classes of the outputs data (average mil...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...l allow manufacturers of insemination doses to improve the quality of their product.
...
Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1
Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
... laparoscopic artificial insemination (LAP-AI) in indigenous ewes in Bangladesh were studied. A total of 78 non- pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; G1 (n = 18; commercial Triladyl®/ 100 × 106 sperm), G2 (n = 21; commercial Triladyl®/ 50 × 106 sperm), G3 (n = 20; home-made tris based/ 100 × 106 sperm) and...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...riment 2, a total of 533 inseminations were carried out by the extender containing best level of arachidic acid (20 ng/mL of extender). In vivo fertility was significantly improved in buffaloes inseminated with semen containing 20.0 ng/mL of arachidic acid (58.64%) compared to control (46.06%). In conclusion, addition of arachidic acid (20.0 ng/mL) in extender significantly enhanced quality and in vivo fertility of post thaw buffalo semen....
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
Atilla Yıldız
...ability of conception at insemination time. Cervical mucus in 36 clinically healthy Holstein cows was evaluated scoring system based on the five variables (colour, viscosity, spinnbarkeit, ferning and pH) describing appearance and characteristics of cervical mucus collected. Each parameter was graded from 0 to 3. Comparison of cervical mucus score including all parameters demonstrated a significant (P < 0.0001) increase between conceived cows’ mean sc...
Summara Abbasi* and Khalid Nawab
...were aware of artificial insemination and vaccination while at least 51% was reported about livestock management. Improved feeding was very active as 70% of farmers were well informed followed by breed status recorded by 70% of the farmers. Demographic results showed that 63% of respondents were literate with an average age of 51 years and farming experience of upto 11 years. Results from the binary logistic model indicate that number of family members, farm k...
Halidou Maiga Nafissatou1*, Abdou Moussa Mahaman Maâouia2, Moumouni Issa1, Marichatou Hamani1
...pine Brown by artificial insemination, is experimented in Niger. This work reports the first results of this introduction on growth and reproduction. Data from 52 crossbreds (75%, 50% and 25% of Alpine Brown blood degree) and 63 Azawak were used for reproduction parameters, and 373 animals for growth parameters. The age at first calving of the crossbred cows (34.25 ± 3.77, 29.74 ± 3.37 and 32.80 ± 3.42 months respectively in the 75%, 50% a...
Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr
...or natural or artificial insemination. There is a relationship between protein profile in BP of buffalo-bulls and buffalo-bull calves which may suggest an early prediction of semen quality in bull calves in future studies.
Keywords | Buffalo-bulls, Blood, Semen, Protein, Fertility.
...
Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6
...e, increase in number of inseminations at each conception and finally culling. The incidence of COD is ranges from 5-30%, due to the improper managemental system, and the prevalence of COD is 10-13% which is associated with selection, heredity, age, environment, improper nutrition, herd size, housing, high milk production, body condition score, lactation period, seasons, retained placenta, stress, metabolic disorders and hormonal imbalance. COD is generally at...
Hamdy Abdala Elnagar1, Wael Mohamed Wafa1*, Abdel-Khalek El-Sayed Abdel-Khalek2
... days-open and number of inseminations per conception. In most postpartum days of all groups, serum P4 and E2 concentrations were in association with conception rate. In conclusion, the dual treatment of lactating cows on day 15 postpartum with 0.6 NAC/kg (orally for 7 days) plus 1% NAC (IU infusion for 3 days) may improve the reproductive efficiency, health performance, antioxidant enzymes, and inflammatory reaction during the postpartum period.
&...
Ghulam Nabi Dahiri1, Akeel Ahmed Memon2, Qudratullah Kalwar3*, Asmatullah Kaka2, Waseem Ali Vistro4, Yar Muhammad Jalbani3, Kiran Nazish3 and Jai Parkash Kolhi5
...rus signs and artificial insemination. Animals of group B and D were treated with OvSynch (GnRH at day 0 followed by PGF2α day 7 and 2nd GnRH at day 9) for estrus synchronization but D was bio-stimulated and B was not bio-stimulated. Animals in group A and C were treated with 2ml normal saline on day 0, 7 and 9 of the treatment. Group C was biostimulated and A was not biostimulated. The results showed that estrus response (60 %) and pregnancy rate (50%) ...
Pharaoh C. Sianangama1*, Brian Tembo2, Sylvia J. Harrison1, Rubaijaniza Abigaba1,3
...15, 22, 30, and 35 after insemination and used at a dilution rate of 1:4. Maize seeds were soaked in different treatment groups, followed by observation for germination and shoot length. Data were analysed descriptively to obtain the means, and inferential statistics including Kruskal-Wallis and Bonferroni tests were employed to explore significance between the means. The Eta coefficient test statistic evaluated the association between selected variables. The ...
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...
Keywords | Artificial insemination, Frozen semen production, Indonesian local cattle, Reproductive performance, Total sperm output
...
Iqra Qazi1, Sohail Raza1*, Masood Rabbani1 and Muhammad Avais2
...als bred with artificial insemination as compare to natural breeding method....
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
...s improved by artificial insemination with fresh and thawed buck semen with all PEE levels, being the highest with PEE (300 mg/buck) and slightly higher for fresh than thawed semen. In conclusion, PEE oral administration (300 mg/buck) for 40 days may be used, as an effective tool, to improve antioxidants capacity, immunity, and quality and fertilizability of fresh and cryopreserved semen of rabbit bucks under HS conditions.
Keywords | Rabbits, Propolis...
Ahmed Fathy1, Rania A. Hassan2, Essam S. Soliman3*
...rate), and days to first insemination (DFI/days). The raw data on 927 buffaloes (763 crossbred and 164 Baladi buffaloes) were collected from a dairy farm in Al Sharqia governorate- Egypt. The results showed that MY/kg, DIM/days, AFC/months, CI/months, DO/days, DP/weeks, SPC rate, and DFI/days were good discriminators between both buffalo breeds. The discriminant function showed a significant association between the predictor variables and groups of dependent v...
Atilla Yıldız* and Ayse Kılıç
...taglandin F2alpha to the insemination day. Control group did not receive resveratrol supplement. The mean erythrocyte GSH-Px levels were nearly similar in both groups before resveratrol therapy. However, at post-therapy, mean GSH-Px concentrations in group T was significantly higher (P<0.05) than that of group C. Likewise, mean erythrocyte GSH-Px concentrations raised significantly (P<0.05) in group T after resveratrol therapy compared to pre-therapy one...
Ghulam Jelani1, Qudratullah Kalwar1*, Asmatullah Kaka2, Abdullah Channo2, Ayyaz Ahmed1, Majid Hussain Soomro1, Hidayatullah Soomro1, Deepesh Kumar Bhuptani1 and Yar Muhammad Jalbani1
...chronization, artificial insemination, superovulation, cloning, embryo recovery and its transfer, in vitro fertilization, cryopreservation and transgenesis. Among these technologies, embryo transfer has achieved a great importance having produced numerous offspring from a genetically superior female. After the first successful transfer of mammalian embryos in 1890, it was approximately 60 years before significant progress became quite noticeable in the basic t...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
...ues including artificial insemination, in vitro embryo production, oestrous synchronization, superovulation, embryo transfer, germ cells or embryos’ micromanipulation, sperm sexing, cryopreservation of ovarian tissues and germ cells and embryos, and genome resource banking for mammalian species. Such biotechnologies allow more offspring to be obtained from selected parents to increase milk and meat productivity, reduce the interval between generations, t...
Hendrikus Fatem1,3, Abdul Latief Toleng2, Muhammad Yusuf2*, Herry Sonjaya2
...pplication of artificial insemination (AI). This study was designed as field research that involved farmers and Bali cows reared under palm oil plantations. A total of 71 heads of Bali cows that have been calving for two consecutive times were used in the present study. The parameters measured were services per conception, length of pregnancy, the interval from calving to the first AI, the interval from calving to conception, and the calving interval. The resu...
Shah Murad Khan1, Hamayun Khan1, Younas Muhammad1, Haq Aman Ullah1*, Muhammad Tariq Tunio2, Ali Gohar2 and Fazli Rabbani3
...ng with timed artificial insemination after 16 hrs. Animals in MSS group were administered with a controlled internal drug release (CIDR) insert on day -7 with simultaneous use of prostaglandin 2-alpha (PGF2-α), GnRH-1 at day -5, removal of CIDR at day 0, concurrent with two doses of PGF2-α at 8 hr. interval, GnRH-2 on day 3, followed by TAI after 16 hrs. Results showed higher estrogen (E2) and lower progesterone (P4) concentrations for MSS than PO...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
...ion rate from artificial insemination was used to determine the fertility rate (AI). This investigation revealed that the quality of the sperms from Bali-polled bulls and Bali-horned bulls did not substantially change after thawing (p > 0.05). However, sperm acrosome integrity was considerably (p < 0.05) higher in Bali-polled bulls than in Bali-horned bulls. In the profiling protein seminal plasma, ZPBP protein expression was found in the Bali-polled bul...
Gulzhan Kalenovna Mussayeva1*, Alikhan Meldebekovich Meldebekov2, Nurgul Alikhanovna Meldebekova2, Gulshat Imanzhankyzy Shaykamal1, Batyrkhan Azimkhanovich Buralkhiyev2, Nurkuisa Mirzabekovich Rametov3 and Kanat Zhumanov4
...to select for artificial insemination bulls from the line whose offspring are most productive and physiologically more adapted to the climatic, technological and forage conditions of the farm. When selecting, special attention should be paid to the strength of the hoofed horn and the duration of commercial use. For the given purposes it is recommended to use such bulls of the Wis Burke Ideal line whose average milk productivity exceeds that of their coevals an...
I Nyoman Sulabda1, Anak Agung Gde Oka Dharmayudha2, I Wayan Nico Fajar Gunawan3, Dwi Fortuna Hashilonda4, Luh Gde Setyawati5, I Ketut Puja6*
...ogs using the artificial insemination method. Eight bitches were used in this experiment. The dog was in good health. The time of artificial insemination was performed 11 days after the onset of proestrus. Bitches are inseminated intravaginally. Pregnancy was confirmed by ultrasound 25 days post-AI. To our knowledge, this is the first successful case of AI in a Kintamani dog. From these results, it can be concluded that free...
Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago
...s a result of artificial insemination with variations in spermatozoa concentrations. Semen from 12 KBC was collected and evaluated to determine their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into 500µl of Ringer’s lactate solution with various concentrations of spermatozoa, namely 50×106, 100×106, and 150×106. Fertile period and fertility were evaluated by ...
Achmad Firman1*, Sondi Kuswaryan1, Lilis Nurlina1, Muhamad Hasan Hadiana1, Marina Sulistyati1, Unang Yunasaf1, Dwi Cipto Budinuryanto2, Iman Trisman3
...ugh a massive artificial insemination program (SIWAB/SIKOMANDAN). Previously, the artificial insemination program was carried out partially and not with the feed, health, and community services programs. However, feed, animal health, veterinary public health, and community services are a part of the SIWAB/SIKOMANDAN program. The purpose of the study was to assess the program performance through several aspects, such as the n...
Trinil Susilawati1*, Aulia Puspita Anugra Yekti1, Amir Firdaus2, Dinda Ayu Damayanti2, Rizki Prafitri1, Nanang Febrianto1, Kuswati1, Achadiah Rachmawati1, Sri Wahjuningsih1, Nurul Isnaini1
...wed the estrus sign, the insemination was repeated with the same treatment and then continued to observe NRR 2 on the next cycle. After two months, the pregnancy and AI failure were observed using ultrasound. A Chi Square was performed using SAS OnDemand for Academics (ODA, Cary, NC, USA). Moreover, probability values were calculated using the least significant different testing. The results showed that NRR-1 was 83.75% and 85%, while NRR-2 was 62.5% and 68.75...
Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*
...led semen for artificial insemination were used to obtain different stage embryos: bovine 8 cell XX embryo, 8 cell XY embryo, 16 cell XX embryo, 16 cell XY embryo, morula XX embryo, morula XY embryo, blastocyst XX embryo, and blastocyst XY embryo. A sequencing library was constructed by the Smart-Seq2 amplification. The transcriptome was sequenced by Illumina HiSeqXten high-throughput sequence technology, and effective sequences were analyzed by functional ann...
Mohamed A. Abu El-Hamd1*, Abdelslam M. Metwally2, Zahya R. Ghallab2, Wael A. El-Hamady1 and Mohamed A. El-karamany1
... G2 and G3 at artificial insemination (AI), while it was higher at days 7, 14 and 24 post-AI in pregnant than in non-pregnant cows. At oestrus, the P4 concentration was significantly decreased in G1, G2, G3 and G4, in pregnant cow than in non-pregnant of high or low fertility. Economic analysis revealed that the PGF2-GnRH protocols were the least expensive (L.E. 65/animal), while the OvSynch treatment was the most expensive (L.E 95/animal)....
Ferry Lismanto Syaiful1*, Jaswandi Jaswandi2, Mangku Mundana2, Ilham Ilham2, Novirman Jamarun1, Efrizal Efrizal3
...t essential aspect after insemination, this can increase reproductive efficiency and buffalo birth. This study is aimed is evaluating the use of mung bean seeds on multiple dosages in the growing seeds tests for the accuracy, sensitivity, and phases of local buffalo pregnancy, and detecting buffalo pregnancy quickly, massively, and cheaper. The object of this study was 40 local buffalo post-AI with the criteria of BCS ≥3 and 2-3 parturition. The research me...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...successful of artificial insemination (AI) in buffalo was the quality of spermatozoa post-thawed. Therefore, research was conducted on the use of lycopene as an effort to improve the quality of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-5...
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
...gn: justify;">Artificial insemination is beneficial for the poultry industry and breeding programs due to the ability to efficiently use superior male chickens for mating with multiple females. This study aimed to examine the effect of adding BHT (butylated hydroxytoluene) into the diluent and storage time on the quality of Gaga chicken spermatozoa during liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled wi...
Ediset1*, Jaswandi2, Fuad Madarisa1, Amrizal Anas1, Rizqan2
...ttle pregnant. The first insemination (Conception Rate) with a C.R. value is 57.9%, and the percentage of birth rate (Calving Rate) with a CvR value is 47.9%. The study concludes that the adoption rate of A.I. in the buffalo breeding business in Padang Pariaman Regency, West Sumatra Province is in the moderate category. However, the success rate of its implementation is good.
Keywords | Innovation adoption, Adoption rate, Pregnan...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...tation. After 45 days of insemination, pregnancy was confirmed utilizing a transabdominal probe and real-time B mode ultrasonography. The results showed that group B had a significantly (P<0.05) higher conception rate (50%) than group A (30%). In conclusion, supplementation of TEY extender with 2 mM selenium improved in-vitro (chilled and frozen-thaw sperm traits) and in-vivo conception rate of Kamohri buck semen. ...
Gokarna Gautam1*, Hari Adhikari1, Bhuminand Devkota1, Subir Singh2
...y fixed timed artificial insemination (FTAI) at 16-20 hours after second GnRH. Buffaloes in Group T2 (CIDR-synch, n=30) were treated as in group T1 except that a CIDR containing 1.9 gm progesterone was inserted into the vagina on d0 and removed on d7. Ovarian cyclicity status during CIDR insertion and the presence of corpus luteum (CL) on 8-9 day after FTAI were determined using transrectal ultrasonography. Estrus expression rate (96.7% vs 68%; P=0.008) and ov...
