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Bruce L. Gordon

... we offer a synoptic critique of young-earth creationism while developing and defending an evangelically acceptable alternative for understanding the relationship between God’s works and God’s words.

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Shahid Ali, Munir Khan

...ied random sampling technique during 2009-10. Stochastic frontier analysis was employed for the estimation of technical efficiency levels and its determinants. Maximum likelihood estimation (MLE) technique was applied to estimate stochastic frontier Cobb-Douglas production function to ascertain the level of technical efficiency. The econometric computer software STATA was applied for the estimation. The estimated value of te...

Gerald Misinzo1,*, Tebogo Kgotlele1, Epaphras A. Muse1, Jan Van Doorsselaere2, Mikael Berg3, and Muhammad Munir4

...ndahimba bordering Mozambique was reported. The aim of this study was to perform molecular typing of PPRV strains that caused outbreak in Tandahimba district in 2011. A total of 17 (sheep=0, goats=17) out of 27 (sheep=3, goats=24) were positive for PPRV N gene. The nucleotide sequence and phylogenetic analysis clus- tered the PPRV from Tandahimba into lineages II and IV. The results give evidence of at least two separate introductions of PPRV into Southern Tan...

Ahmed Sultan Jatoi1*, Shahid Mehmood2, Jibran Hussain2, Hafiz Muhammad Ishaq2, Yassar Abbas3, Muhammad Akram2

 

...e analysed by ANOVA technique and means were compared by using DMR test. The results of the present study showed that the weekly mean body weight (g) of Japanese quail birds obtained from the four strains designating as M, K, S, and Z showed non-significant differences at week 0 and 1, whereas, a significant effect was found at week 2, 3, 4, 5, and 6. The average weight gain (g) was found non-significant at week 1 and week 6, however, the statistical analysis ...

Aqeel Ahmad, Zammurad Iqbal Ahmed, Irfan Aziz*

 

...ysis was a reliable technique for farmers to optimize fertilizer recommendations and harvest maximum returns from their investment in agricultural business.

 

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Hai-Bo Wang, Qiu-Hua Mo, Ze Yang*

...nd molecular beacon technique. Recently there have been two major progresses in the field, in which RT-NASBA technique was applied for the detection of diarrhoea related viruses. This mini review overviewed the basic principle of RT-NASBA and discussed the significance of these new findings.

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Hayat Badshah1*, Farman Ullah1, Abid Farid2, Ahmad-U-Rahman Saljoqi1, Sajjad Ahmad3

...-rearing production techniques of Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae) (a parasitoid of the cotton mealybug) in the Laboratory, it is necessary to know the development capacity of the cotton mealybug, Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae), on different host plants. Such studies would identify the best host plant for its efficient rearing. The present study investigated some basic life table parameters i.e. female life s...

Kemi Funmilayo Omotesho1*, Isreal Ogunlade1, Opeyemi Eyitayo Ayinde2

...age random sampling technique. Data used for the study were analysed using descriptive statistics, Likert scale and the regression analysis. The study revealed that farmers perceived the level of accountability of extension to farmers in the study area as poor. Level of education, access to training and membership of farmer-groups negatively influenced accountability at p<0.05. Based on the findings of the study, it is recommended that agricultural extensio...

Faheem Khan*, Muhammad Zafarullah Khan

...ne using scientific techniques, paying special attention to those who are illiterate, to the point of developing special extension tools for those who cannot read.

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Shahid Iqbal Awan1*, Syed Dilnawaz Ahmad2, Muhammad Amjad Ali3, Muhammad Shahzad Ahmed1, Aurangzeb Rao1

...eight. Multivariate techniques, including factor analysis (FA) and cluster analysis, indicated that the magnitude of variability existing in the gene pool was satisfactory for initiating a breeding plan. Relative water content, cell membrane stability, osmotic adjustment and grain yield per plant were major contributors towards variability. Grain yield per plant was closely linked to relative water content, cell membrane stability and specific flag leaf area. ...

Brittany D. Rife 1,2, Marco Salemi 1,2*

E-mail | salemi@pathology.ufl.edu

...scale, creating a unique opportunity for the application of phylogeography at the intra-host level, which has until recently been limited by dataset quality and the lack of a rigorous statistical framework. As recently described in studies using the SIV-macaque model of neuroAIDS, the incorporation of
spatial, temporal, and demographic inference into intra-host evolutionary analysis using Bayesian phylogeography allows for inference ...

Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*

...the efficacy of two techniques i.e. serum neutralization test (SNT) and solid-phase competitive ELISA (SPCE) for foot and mouth disease virus (FMDV) structural antibodies detection in terms of their sensitivity and specificity. These methods were performed by using set of sera collected from cattle with foot-and-mouth disease vaccination. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2...

Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah

Email | mmshah@ciit.net.pk

...Various methods and techniques have been developed and tested to improve important cereal crop species since quite long time. Among the prominent techniques, traditional plant breeding has been on leading edge. The discovery of molecular genetics and biotechnological techniques, such as genome mapping, tissue culture, genetic transformation and in vitro regeneration offered&...

Muhammad Arif Zafar1*, Arfan Yousaf1, Murtaz-ul-Hasan1, Faisal Ayub Kiayani1, Matee ul Islam2 and Muhammad Farooq Iqbal1 

...-to-end anastomosis technique in a calf treated for traumatic umbilical hernia with peritonitis. A cross-bred calf was presented with peritonitis due to traumatic umbilical hernia. The decision to operate the calf was made after confirmation of umbilical hernia resulted in septic shock. Animal was first stabilized with supportive treatment prior to surgical decompression. A modified two-layer hand-sewn anastomosis technique ...

Rehmat Ullah1*, Muhammad Zafarullah Khan1 and Kalim Ullah2

...on Sekaran sampling technique while using descriptive research design. Well-prepared and pre-tested interview schedule was utilized for collection of data. Descriptive statistics i.e. frequency, percentages and rank order was calculated. Chi-square test was performed to find out association among demographic characteristics and MFSC registration duration and Rank Based Quotient was calculated for problem identification. It was found that machinery was provided...

Abiodun D. Olabode

...and farm management techniques employed in the study area were observed. The study employed simple percentages to analyse the farmers’ responses; while regression model established significant relationships between rice yield and farm management methods adopted in this area. Results were presented using charts and tables. The study revealed that; chemical fertilizers, manual weeding and shifting cultivation are essential for rice cultivation in the study...

Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3

... phase High Performance Liquid Chromatography (HPLC. Out of total samples Aflatoxin B1 was detected in 33 feed samples, with a percent contamination rate of 83%. The level of aflatoxin B1 ranged from 0 to 225.736 ppb in different feed samples. The maximum level of aflatoxin B1 was detected in cotton seed cake (mean level 137.059 ± 22.293 ppb) followed by wanda, homemade concentrate mixture and wheat bran.. Amongst the positive concentrate feed samples, ...

Murtaza Ali*, Yasser Durrani and Muhammad Ayub

...elected dehydration techniques sun drier cabinet drier, tunnel dryer, solar drier and portable solar dryer, on the white mulberry fruit (Morus alba) variety grown in District Skardu, Gilgit Baltistan. After drying, mulberry fruit was stored for 150 days and evaluated for physiochemical and sensory parameters (water activity, ash content, percent acidity, pH, moisture content, reducing and non-reducing sugars, taste, texture, color and overall acceptabil...

Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*

 

...red to the F gene. Two unique amino acid substitutions (K423Q and E426G) were observed in Bangladeshi and Chinese isolates in the partial sequence of N protein.The recent Bangladeshi isolates are somewhat divergent from the earlier Bangladeshi isolate, indicating that the PPRV strains in Bangladesh are continuously changing their genetic character.

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Jannatul Noor, Md. Ahaduzzaman, Mir Md. Afzal Hossain, Mohammad Alamgir Hossain, Sardar Abdur Rahim and Md. Samun Sarker

...m positive sampling technique. Among the breed, Black Bengal goats were highly affected (53.33%) followed by Jamuna pari (33.33%) and cross breed (13.33%) (P=0.00). Black coat coloured (53.33%) were mostly infested than another coat coloured animals (P=0.00). Age less than 6 months goats were mostly affected (55.0%) than adult goats (26.67%) (P=0.00). In this study, males were more prone (58.33%) than female goats (41.67%) (P=0.036). The ticks were more in goa...

Mazhir Nadeem Ishaq, Li Cui Xia, Rukhsana Rasheed, Zeeshan Ahmad, Muhammad Abdullah

...embers. Econometric techniques to draw inferences include the application of t-test, binary Logistic model and Propensity Score Matching (PSM) with algorithm of near neighbor (NN) and interval matching. The impact of cooperative membership on small milk producers was evaluated by calculating the values of average treatment effects (ATE) on eight performance indicators. The outcomes of study disclosed that cooperative members have higher farm income, more milk ...

Haidar Ali, Malik Muhammad Shafi and Himayatullah Khan

...ing random sampling technique and data were collected through a pre-tested interview schedule. This study is focused on two selected districts of Peshawar and Charsadda. Four villages were studied two each from the sampled districts. Analysis showed that owners (59.31 percent off-farm employment) were performing more off-farm jobs than the tenants (50.74 percent off-farm employment) and owner-cum-tenants (41.77 percent off-farm employment). The dominancy of ow...

Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand

...ment (SELEX)” technique. Among the receptors available for biosensing, aptamer exhibit the advantages of high specificity, selectivity, stability, facile labelling and modification, which makes them ideal candidates for development of new biosensing applications for detection of specific target molecules. In the present review, we concentrate on the recent advances in the development of aptasensors for antibiotics residue analysis based on electrochemica...

Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*

...aws were then kept over liquid nitrogen vapours for 10 min and plunged in liquid nitrogen for storage. Thawing was performed after 24 h of storage, at 37°C for 30 seconds and post-thaw sperm quality parameters were assessed. Sperm progressive motility, plasma membrane integrity, acrosomal integrity, viability and chromatin integrity was higher (P<0.05) in extender containing melatonin 0.1 mM and 0.5 mM compared to con...

 Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5

... of mass production techniques in Pakistan, the development and application of entomopathogenic nematodes (EPN) depend on the use of host insects for in vivo production. As there is little information regarding relationship between nematode dosage and production of infective juveniles (IJ) of Steinernema feltiae and ability to locate hosts, therefore, in the present studies investigations were done on these aspects. A significantly greater emergence of IJ of S...

Abdur Rahim1, Ghulam Abbas1, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Noor Khan4, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6

... But the processing technique should be improved to match international standard, and to avoid declining in production of fish meal strategic management of fisheries resources will be required.

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Abdul Mannan Babar1* and Abdul Hannan Nagi2

...ng of eight refind techniques, best Keloid was produced by the technique where Transforming Growth Factor Beta 1 injection was followed by ventral skin, ventral perichondrium, cartilage, and dorsal perichondrium punch excision, leaving behind only dorsal skin.

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Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*

... other animals in this unique region of the world.

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John G. Bruno

...etic identification techniques such as PCR or gene probe hybridization.

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Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*

...ment, we established a unique murine model of femoral artery injury through a home-made wire. The mouse model of vascular injury may be used to explore the molecular mechanism of post-angioplasty restenosis with lowest costing.

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Xiangxing Zhu1, Junyu Nie1, Shouneng Quan1,2, Huiyan Xu1, Xiaogan Yang1, Yangqing Lu1, Kehuan Lu1 and Shengsheng Lu1*

...ll nuclear transfer technique, we successfully generated transgenic Guangxi Bama mini-pigs carrying a fluorescent protein (DsRed) reporter gene regulated by the 2.2-kb human glial fibrillary acidic protein promoter (hGFAP-DsRed). This study characterized transgene expression in such transgenic Guangxi Bama mini-pigs and their offspring. Our findings indicate that the hGFAP promoter contains matching regulatory elements for directing specific expression in porc...

Oluwasogo David Olorunfemi, Oluwasegun Adetokunbo Adekunle, Felix Olayinka Oladipo, Temitope Oluwaseun Oladele and Oladimeji Idowu Oladele

... two-stage sampling technique to select one hundred and sixty respondents for the study. A purposive selection of two Local Government Areas each from the two Agricultural Development Programme administrative zones (Zones C and D) in Kwara State where fish farming is prominent and well-practiced was carried out. Forty fish farmers were then randomly selected from the fish farmers’ association chapter present in each selected Local Government Area. Interv...

Hina Fatima, Lal Almas and Bushra Yasmin

... innovative farming techniques must be implemented in an efficient manner. Effectively implementation of agricultural schemes, formal education and in-formal education should be realized. During and after conducting the agricultural research, it is needed to recommend the farmers about the rate of inputs application i.e. seed, pesticide spray and NPK that are best suited to the local weather patterns and cropping systems.

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Naushad Khan1*, Shahnaz Akhtar1, Munir Khan1, Shaista Naz2, Javeria Tanveer1 and Muhammad Kaleem3

...ulti stage sampling technique. At first stage, all the three tehsils (i.e.; Mardan, Takht Bhai and Katlang) of the district were selected. At second stage, two villages from each tehsil were randomly selected. In third stage, all the beneficiaries of ZTBL credit program for maize crop was selected and interviewed. Descriptive statistics, paired t-test and correlation analysis were used to analyze the data. It was found, that ZTBL credit program has overall a p...

Zaffar Malik, Moazzam Jamil, Ghulam Hassan Abassi, Muhammmad Nafees, Muhammad Rafey and Muhammad Kamran

...ike plastic limit (PL), liquid limit (LL) and plasticity index (PI), tensile strength (TS), shear strength (cohesion (C) and angle of friction (φ)) were studied. Results showed that amendments had improved soil physico-mechanical properties. No specific trends were observed for consistency limit, however, PI decreased for all the amendments applied and LL showed correlation with φ and TS. Significant reduction (p≤ 0.05) in the coefficient of linear ...

Muhammad Afzal1 and Murad Khan2*

... purposive sampling technique. From these tehsils, 92 farms were selected randomly from 460 poultry farms. The sample farms were categorized into three groups i.e. small, medium and large. For analysing the data, descriptive statistics and gross margin were used. The average total cost of small poultry farm was Rs. 3,10,067 per flock while that of medium and large farms were Rs. 4,99,002 and Rs.7,34,290 respectively. The highest cost was estimated for feed fol...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*
...olecular biological techniques provide a useful alternative approach. Concerning with genotypic identification, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was applied for identification of Candida species, Cryptococcus albidus, Saccharomyces, Trichosporn, Torulopsis and Rhodotorula. Using universal primers ITS1-5.8S-ITS4 amplified ITS region. And it yielded a unique PCR size of approximatel...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...s, proving that the technique employed was effective and of simple execution for the treatment of dorsal patellar fixation in bovine.
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Waqar Islam*1, 2, Madiha Zaynab3, Muhammad Qasim2 and Zujian Wu1,2
... molecular breeding techniques via utilizing advanced molecular markers involving cis and trans genetics. The review adhere recent research findings regarding disease resistance against viral diseases and concludes via shedding light upon the future prospects in this exciting field of research.
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Zafar Iqbal1* and Muhammad Khurshid2 
...) is very versatile technique that has the potential to expedite the detection of plant viruses from plant species containing various forms of PCR amplification inhibitors. In current study, IC-PCR was used for the detection of Tomato leaf curl New Delhi virus (ToLCNDV) from plant extracts of Nicotiana benthamiana plants inoculated with ToLCNDV. To immunocapture the ToLCNDV, two antisera raised against Tomato yellow leaf curl virus-coat protein (...
Nadeem Rashid1,*, Masroor Ahmad Bajwa1, Mohammad Masood Tariq1, Tanvir Ahmad2, Majed Rafeeq1, Zafar Ahmad1, Mohammad Arif Awan1, Mohammad Ali1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Asad Ulah1 and Amanullah Khan3
... using spread plate technique. Identification of fungal isolates was carried out on the basis of cultural and morphological characters by using slide culture technique. While mean moisture percentage was recorded as 5.3% with a significant difference (P<0.05), the pH was noted to be slightly acidic with non-significant difference (P>0.05) among seasons. Overall mean viable fungal count was 4.72 × 103&p...

 Saqib Ali1, Suliman Ali1, Lina1, Wen Zhou1, Muhammad Irfan Waris1, Ashfaq Ali2 and Man Qun Wang1,*

...addition to biological uniqueness and development of sawyer beetles (Monochamus alternates) are also discussed.
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Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
...ce Focus Inhibition Technique (RFFIT) revealed mRVNA titer (>0.5 IU/mL) in 29 puppies while 48 puppies showed mRVNA titer less than minimum protective level (0.2-0.4 IU/mL). RFFIT did not reveal mRVNA in 23 puppies that were declared lacking maternal rabies immunity. One basis of findings of this study it was concluded that puppies population lacking maternal rabies immunity is one of the responsible factors for increasing incidence of rabies in humans and ...
Muhammad Tahirand Memoona Shehzadi*
...nvironment friendly techniques. Present study was proposed to evaluate theresponse of spring and autumn sunflower to chemical and bio-fertilizers for yield and quality traits during 2014 at University of Agriculture, Faisalabad, Pakistan. The treatments comprised on the combination of chemical fertilizers (No chemical fertilizer, half recommended dose of fertilizer and full recommended dose of chemical fertilizer) and Plant Growth Promoting Rhizobacteria (PGPR...
Abdus-Subhan1, Qudratullah Khan1, Muhammad Mansoor2, Muhammad Jamil Khan1, Ammanullah2, Matiullah Khan3
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
... using High Performance Liquid Chromatography (HPLC). The chlorogenic acid was isolated using resin. The recovered amount of compound was calculated. Aliquots of the extracts at known concentration of chlorogenic acid were then applied against different bacterial strains for antibacterial activity. The minimum inhibitory concentration of isolated compound was also measured. Two antibiotics were used as references compounds f...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...nucleic acid-based) techniques have revolutionized the diagnosis of plant viruses. While the technologies available to virologists have obviously become more diverse and improved, the challenges have also changed greatly.Detection of plant viruses is becoming more critical as globalization of trade,particularly in horticultural commodities increase. The potential effects of climate change have further aggravated the movement of viruses and their vectors, trans...

Neerja Agrawal, Mukesh Srivastava, Akhilesh Tripathi and Amrendra Singh

Survey and monitoring of pests, parasites and predators of pulse crops in central and eastern Uttar Pradesh
...terpillar, Spilarctia obliqua, plant hopper of Pentatomorpha group, termite Odontotermes spp, and cut worm Agrotis ipsilon in chickpea whereas Campoletis chlorlidae was recorded as natural enemy feeding on H. armigera larvae. In pigeonpea crop, mainly H. armigera, leaf webber Grapholita (Cydia) critica, Myllocerus spp, spotted pod borer Maruca testulalis, plume moth Exelastis atomosa, tur pod bug Clavigralla gibbosa, jassid Amrsca bigutulta, termite Odontoterm...

K.N. Ahmed, S.H.A. Pramanik, M. Khatun A. Nargis M. R. Hasan

Efficacy of plant extracts in the suppression of insect pests and their effect on the yield of sunflower crop under different climatic conditions
...ntered were Spilosoma obliqua (Walker) (Lepidoptera: Arctiidae),Homoeosoma sp. (Lepidoptera: Phyticidae),Heliothis armigera (Hb.) (Lepidoptera: Noctuidae), Monolepta signata(Oliv.) (Coleoptera: Chrysomelidae) and Epilachna septima Dieke. (Coleoptera: Coccinellidae) . The efficacy of five kinds of plant extracts or botanicals viz., Neem (Azadirachta indica) seed oil, castor ( Ricinus communis) oil, a mixture of Neem seed oil and sesame oil, leaf extracts of cus...

B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran

Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
... state fermentation and liquid state fermentation. In solid fermentation, the fungus is grown on various cereal grains, agricultural wastes and byproducts. The solid state production is highly labour intensive and fit for cottage industry. These products are used mainly for direct soil application in nurseries/main fields to suppress the soil-borne inoculum. In liquid state fermentation, Trichoderma is grown in inexpensive m...

1S. A. Khan and 2S. Jha

Effect of dates of sowing, moisture regimes, varieties and weather factors on incidences of aphid, Lipaphis erysimi (Kalt.) in rape and mustard
...on was highest during siliqua formation phase due to prevalent conducive weather conditions, followed by reproductive and vegetative phases over all varieties. Weather conditions associated with critical period of infestation remained in the range of 21.7-28.9 oC, 7.3-17.8 oC, 14.5-23.3 oC, 78-98 %, 29-73 % and 53-85 % for Tmax, Tmin, Tmean, morning RH, after noon RH and RHmean, respectively. Tmax, Tmin, Tmean and degree-days showed consistently significant ne...

A. K. Chaubey and Satyandra Kumar

Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...ilution and cultured in liquid protein supplemented broth medium and culture filtrates (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual culture of wilt fungus with antagonist fungus and bacteria isolates in PDA media plates. In in-vivo test soil was amended with antagonist and wilt fungal and bacterial isolates 2 x 106 CFU/ml and 2 x 108 CFU/ml respect...

S. K. Dutta, S. Roy Chowdhuri, D. Pandit and A. K. Bajpai

Rot diseases of muga host, Som (Persea bombycina) in Assam, India
...ate in India. India is unique in producing the golden muga silk and has monopoly in global silk market grows up to 20-25 meters and survives for 18-20 years. Pollarding of Som plant at 6 ft height is a common practice in North Eastern region of India for controlled rearing (outdoor) of muga silkworm. It was observed that stems of som plant (specifically the pollarded plants) were generally infected with rot disease when it grows older. Phellinus contigus (unde...

M. S. A. Mamun and M. Ahmed

Integrated pest management in tea: prospects and future strategies in Bangladesh
...l measures, genetic techniques and sex pheromones technology. In tea husbandry, cultural control measures such as plucking, pruning, shade regulation, field sanitation, fertilizer application, manipulation or destruction of alternate hosts and selection of pest resistant/tolerant varieties and mechanical mechanisms like manual removal, heat treatments, light traps, use of bio-pesticides, bio-control agents and sex pheromone trap need to be given more importanc...

P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi

Botanicals for control of anthracnose of bell peppers
...ed by poisoned food technique, seed germination and under green house experiments for control of Colletotrichum capsici, fungal pathogen responsible for anthracnose disease in bell peppers (Capsicum frutescence L.). The results showed that all the selected plants have potential to inhibit the radial mycelial growth of C. capsici in-vitro. The plant extract from C. aromaticus at 50% concentration showed 42% radial mycelial growth inhibition in poisoned food tec...

Madhuban Gopal, Rajesh Kumar and Arunava Goswami 

Nano-pesticides - A recent approach for pest control
...using encapsulation technique. The Dynamic Light Scattering (DLS) studies, Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) studies confirmed the nano-structure of nanopesticides. Nanoparticles of sulphur and hexaconazole thus characterized were estimated for the presence of active ingredient in the formulation adopting analytical techniques like Gas Chromatography / High Pressure L

A. Sajeena and T. Marimuthu

Efficacy, stability and persistence of Ganosol, a Ganoderma based fungicide against plant pathogens
...ial by dual culture technique against sheath blight pathogen of rice was developed as an emulsifiable concentrate. The formulation of 10EC at 0.25% was standardized as the best emulsifiable concentration for use against the pathogen. The pre inoculation spray of the formulation was observed to produce 63.62% reduction in lesion height caused by the sheath blight pathogen. Two isoforms of poly phenol oxidase, PPO 1 and PPO 3 were observed to be induced only in ...

Shams Ur Rehman, Muhammad Arif and Abdul Mateen 

 

Soil-borne viruses in major potato growing areas of Pakistan
...cal and serological techniques. Biological characterization of the field isolates revealed that variability in the symptoms produced by them on host plant. The interaction and association of vi-ruses (PMTV and TRV) and their vectors was calculated by using Jaccard index of similarity. The association of PMTV to its vector Spongosporsa subteranea in Hazara division was found 27.0% while their association in Malakand division was found 24.4%. The TRV associated ...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
... using the fast protein liquid chromatography. The lactoferrin was further characterized through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Disc diffusion method was applied for the evaluation of antibacterial activity of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lacto...
Obaidullah1, Zafar Ali Shah1* and Sami Ullah2
...umn chromatographic technique. Characterization of Lapachol was carried through melting point assessment and running its comparative TLC against standard, added with structure elucidation by FT-IR spectrum. Silver nanoparticles of Lapachol were  synthesized by treating 1 mM silver nitrate (AgNO3) solution with 1 mM solution of Lapachol in the presence of sodium hydroxide (0.1 N) in double distilled water at pH of 7.6 having temperature 40 °C for 3 hou...

Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1 

...isolates contained the unique signature of LSDV (A11, T12, T34, S99 and P199). Phylogenetic analyses showed that the GPCR genes of LSDVs identified from ticks were closest genetically to the previously detected LSDVs from infected ruminants, indicating a potential role of Ixodid ticks for transmission of LSDV. This study showed the role of A. hebraeum ticks for transmission of LSDV. So, tick control is a crucial part, which should be included as a part of LSDV...

Umer Wahid1, Shahid Ali2* and Nihal Ahmad Hadi3

...multistage sampling technique. An interview schedule was used for data collection during November-January, 2016. Maximum likelihood technique was used for the estimation of the Cobb-Douglas stochastic frontier production model. After running various econometrics software the output of the Frontier 4.1 was found good fit. The findings of the study showed that technical efficiency ranges from 0.83 to 0.99 with the mean technic...
Sogolo L. Lebelo1,* and Gerhard van der Horst2
...lectron microscopic techniques and to elucidate the association of these cells with other testicular cells. The Leydig cells occur as a group of polymorphic cells in the intertubular region, and are usually associated with large and small blood vessels and abundant loose connective tissue. Leydig cells have large spherical or slightly elongated nuclei, which possess prominent nuclei and frequently deep indentations of nuclear envelope. The nuclei are character...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and  Kui Chen2,*
...ugu fasciatus is a unique species of China which is distributed in the estuary of the Yangtze River, and belongs to the same genus with Red fin puffer T. rubripes which has been used as one of human genome model animal since mid-1990s. In this experiment, we cloned a pair of DNA fragment sequence including complete ORF (921bp) of taste receptor candidate gene T2R1 of T. fasciatus and studied the differential expression spectrum of tiss...

Muhammad Ibrahim and Ahmad Khan*

...he role of mulching techniques and nitrogen management in crop stand and phenological occurrence is not well understood. Therefore field studies were conducted at Agronomy Research Farm, the University of Agriculture Peshawar-Pakistan to quantify the effects of mulching techniques and nitrogen management on maize (Zea mays L) phenology and crop stand in 2013 and 2014. Treatments included mulching techn<...
Rahat Naseer1*, Abu Saeed Hashmi1, Zulfiqar-ul-Hassan2, H. Rehman1, Saima Naveed1, F. Masood1 and M. Tayyab1
...ifferent processing techniques using acid, alkali, water treatment and fermentation to prepare processed moiety on pilot scale. All these processed materials were included in experimental ration at a fixed level (20 %). All iso-caloric and iso-nitrogenous rations were offered to experimental animals (n=36, 22 male 14 female Lohi sheep almost evenly distributed in experimental groups) having almost uniform body weight (BW). During 60 days of the trial, no signi...
Muhammad Aslam Farooqi1,*, Mansoor-ul-Hasan2, Sohail Akhtar1Muhammad Arshad2, Muhammad Naveed Aslam3 and Muhammad Rafay4
... using High Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) was used to determine residues of commonly used agricultural insecticides (imidacloprid, thiametoxam, profenofos, endosulfan, spinosad and deltamethrin) in multi-floral raw honey of Apis dorsata F. collected from the cotton belt area of Punjab, Pakistan. The residues of these insecticides were extracted using ethyl acetate. Honey samples were spiked at 0.1 and 0.01 mg/kg....

Wali Khan1, Naushad Khan2 and Shaista Naz2*

... purposive sampling technique a sample size of 80 bee keepers was selected from the three tehsils of the agency (i.e; Khar, Salarzai, and Nawagai). The data were collected from the sampled bee keepers using a pretested questionnaire by face to face interview in 2014 production period. The collected data were analyzed using frequencies, percentages, descriptive statistics, rank orders and paired t-test. Findings revealed that bee keeping is one of the important...
Muhammad Tariq Saeed1*, Muhammad Ashfaq Wahid1, Muhammad Farrukh Saleem1, Mumtaz Akhtar Cheema2, Muhammad Shahid1, Abdul-Shakoor1, Abdul-Sattar3
...1) and five priming techniques (control, on-farm priming, hydro priming, hydration inoculation and osmo priming with 0.5% urea solution. Results revealed that hydro priming, on-farm priming and hydration inoculation significantly reduced the time to 50% emergence (E50), mean emergence time (MET) and emergence index (EI) of both soybean varieties. Moreover, osmo priming had no significant effect on E50, MET and EI that was at par with cont...
Shantanu Jha1, A. Rama Devi1, Ngalaton Kasar1 and Abhishek Mukherjee2
...sup> August provided a unique platform to scientists working in the eastern and north-eastern parts of India on fruit flies to interact, share their experience and finally to prioritize areas for future Research. The meeting started at 12:00 noon on 7th of August and continued till 5:00 pm and was attended by 31 participants. The programme was inaugurated by Prof. D. D. Patra, Hon’ble Vice-Chancellor, Bidhan Chandra Krishi Viswavidyalaya (BCKV...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...elopment. Different techniques have been used to determine the integrity of DNA in which acridine orange (AO) assay is easy and less costly. Though used in many species but the variability exists in technique. Therefore, the present study was aimed to optimize the steps of AO staining protocol for buffalo sperm which has not been explored yet. Semen was collected from two mature buffalo bulls, diluted in tris citrate egg yol...
Rana Muhammad Aadil1,2*, Xin-An Zeng1, Saqib Jabbar3, Akmal Nazir2, Abid Aslam Mann2, Muhammad Kashif Iqbal Khan2, Abdullah2, Amna Ramzan2
...re processing (HPP) techniques on some quality parameters of grapefruit juice were investigated based on pH, acidity, ºBrix, viscosity, cloud value, hunter color values, bioactive compounds, microorganisms and enzyme analysis. The grapefruit juice was treated with processing variables of HPP treatment (0, 150, 200 and 250 MPa), temperature (40, 50 and 60 oC), for processing time of 3 minutes and thermal treatment was done in a water bath at 70 ...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1,  A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
... straws and stored into liquid nitrogen for 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm characteristics such as sperm motility, viability, morphology, membrane integrity, and acrosome integrity were decreased in treated groups. DHA concentration was increased in all supplemented groups. Whereas ALA...
Rehmat Ullah1*, Kalim Ullah2, Inam Ullah3, Muhammad Zafarullah Khan1 and Asif Nawaz1 
...Rank Based Quotient technique depicted that the top priority problem identified in the study area was the scarcity of extension staff. It is recommended that public financial support should be increased which are working with same interests, to have a better Extension Service in place, functional, operational, adequate and with enough availability of staff. Furthermore funding must be improved, for proper infrastructure and latest communication technologies so...

Abbas Ullah Jan1, Muhammad Fayaz1*, Yousaf Hayat2, Dawood Jan3 and Ghaffar Ali3 

... a valid estimation technique for fruit consumption in Pakistan. The results show that quantity elasticity with respect to income is constant for dates, grapes, guava and mango and tends to increase in all fruits, apple, banana and citrus with increase in household income for urban households. For rural households with increase in household income, quantity elasticity of all the fruits tends increase with the exception of grapes where it decreases and remains ...

Shahab e Saqib1*, Himayatullah Khan2, Sanaullah Panezai3, Ubaid Ali4 and Mazhar Ali5 

...Multistage sampling technique was used to select the study area. A standardized questionnaire was used to collect data from farming households’ heads. Credit fungibility ratio and ANOVA were used to explore the credit fungibility and credit margin of investment among the groups of farmers. Results showed that a considerable proportion of loan was used in non-agricultural activities by all farmers in general and small farmers in particular. The findings o...

Sikandar Hayat, Khalid Usman* and Muhammad Tahir Amin 

...resource conserving technique, however, drill clogging and immobilization of nitrogen are problems in the standing stubbles of the previous crop. Row adjustment and an appropriate N fertilizer application method may be a possible solution. Field trials were carried out to explore suitable row spacing and N fertilizer application method for zero tillage wheat at Agronomic Research Area, Faculty of Agriculture, Gomal University, Dera Ismail Khan (D. I. Khan), Pa...
Mubashir Hussain1,Safdar Ali1, Muhammad Naveed Tahir1, Ghulam Abbas Shah1, Ijaz Ahmad2*, Muhammad Aqeel Sarwar3 and Sohail Latif4
...indi to compare the techniques for determination of soil weed seed bank during winter 2012-2013 under rainfed conditions of Pothowar. Soil samples for the weed seed bank analysis were taken from the experimental field before wheat sowing from 0-10 cm, 11-20 cm and 21-30 cm soil depth. Two soil weed seed bank determination techniques were compared viz., sieving method and seedling emergence method. The data were collected on ...
Muhammad Qaisar Nawaz
...st-effective sowing technique for oat forage production under salt affected conditions. Two sowing methods i.e. broad cast and drill sowing with 30 cm apart rows and four nitrogen levels (75,100,125 and 150 % of N recommended dose i.e. 150kg ha-) were tested. Recommended dose of PK fertilizer (85-60 PK kg ha-1) was used uniformly with experimental N rates. Data on plant height (132.00 cm), number of plants (91.33 m-2), number o...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2 
...d by immunoblotting technique. Bubaline placental lactogen was purified to homogeneity and showed a molecular weight of about 30 kD. Purified placental lactogen was administered to male albino mice for up to eight weeks for growth promoting activity. Increased mammary growth was observed in test female non lactating mice, as compared to controls when placental lactogen was applied. Increase weight gain for visceral tissues like kidney, liver, muscle and lungs ...
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*
... and bioinformatics technique were used for characterize the differential expressed proteins. Almost 78 patho-stress responsive proteins which expression level more than 1.5-fold were identified, where 50 proteins were up-regulated and 28 proteins were down-regulated; The identified proteins were categorized into 16 classes, which are mainly including energy metabolism, gene expression regulation, oxidation-reduction reaction, cellular component and stress res...

Haidar Ali* and Malik Muhammad Shafi 

...ing random sampling technique and data were collected through a pre-tested interview schedule. This study focuses on two selected districts i.e. Peshawar, and Charsadda. Four villages were selected two each from the sampled districts. Analysis showed that the coefficients of household size, educational level of the sampled respondent, age of the sample small farmer, were positively related to the off-farm employment. While farm area operated was negatively but...
Fahad Tanveer1, Adnan Afzal2, Muhammad Adeel3*, Sana Shahid4 and Maham Masood5
...onvenience sampling technique who fulfilled the specific inclusion and exclusion criteria were randomly allocated to three groups; group 1 received SNAGs, group 2 Maitland mobilization and group 3 conventional treatment. Baseline, post-intervention and follow up readings were taken through numeric pain rating scale (NPRS) for pain intensity, neck disability index (NDI) for functional status,goniometer for range of motion and manual muscle testing for muscle st...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
Shumaila Ashfaq1, Liaqat Ali2*, Muhammad Ashraf Zia2, Rizwan Ahmad Khan2 and Mehtash Butt3
Anan Kenthaoand Pornpimol Jearranaiprepame*
...ariate morphometric technique to identify fishery management units of C. apogon from six populations of three different river drainages: Pong, Chi, and Mun Rivers. Thirty-two truss measures and standard length were obtained using digital calliper from 291 fish individuals, and raw measured data were then subjected to allometric equation to remove size-dependent variation prior further statistical analyses. Multivariate analysis of variance (MANOVA) indi...

Muhammad Iqbal1, Khalid Usman2*, Muhammad Munir3 and Muhammad Sohail Khan

...le strand placement technique was used in this study. Strands were cut from a freshly opened male spathe and 2 – 3 strands were placed between the strands of the female inflorescence. Data were collected for fruit set (%), fruit drop (%), fruit yield (kg palm-1) and wide range of physico-chemical quality parameters. Results revealed that pollination times affected all the parameters significantly. Maximum fruit set of 98.12, 93.14% and fruit yield of 79....
İbrahim Aytekin1,*, Ecevit Eyduran2, Koksal Karadas3, Rifat Akşahan4 and İsmail Keskin1
...rametric regression technique was implemented. To measure predictive accuracy of MARS, model evaluation criteria such as coefficient of determination (R2), adjusted coefficient of determination (R2ADJ), SDRATIO andPearson coefficient (r) between actual and predicted values in fattening final live weight were calculated. To reveal the highest predictive ability in the MARS algorithm, numbers of terms and basis functio...
Muhammad Siraj-ud-Din1,2, Riaz Aziz Minhas1, Usman Ali3,*, Mayoor Khan2, Muhammad Siddique Awan1, Nuzhat Shafi1 and Basharat Ahmad1
...using scan sampling technique and also collected from local people, hunters and shepherds (n=78). During scan sampling, focused feeding animals were observed with the help of a telescope and spotting scope. Ladakh urial preferred montane dry sub-tropical scrub zone habitat with 41.87% evidences of its presence followed by alpine meadows/alpine scrub zone (21.14%), sub-alpine scrub zone (13.41%), and dry temperate coniferous forests (8.54%). On the other hand, ...
Abu ul Hassan Faiz1,*, Fakhar-i-Abbas2 and Lariab Zahra Faiz2
...lying line transect technique in wild area by using over 3840 trap nights in eight type of soil (sand, loamy sand, sandy loam, sandy clay loam, loam, clay loam, silt loam, silty clay loam). A total of 1571 animals were captured belonging to species of Nesokia indica, Mus musculus, Mus budooga, Millardia meltada, Tatera indica and Golunda elliotti. The maximum number of rodents was captured in loamy soil type with population density (53.3±1...
Ozgur Altan*
...eavored to separate the liquid part to the extent possible and to store them separately. At the end of the study, the fatty acids content in the liquid part, the nutrient content, amino acid and fatty acid profile in the solid part have been analyzed. According to the results obtained, it was observed that the solid part could not be used commercially by substituting the fish meal in a rate of one-to-one, and that it could b...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...s time. Several lab.techniques are developed for rapid detection of FMD but these are expensive and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple and accurate test for the rapid diagnosis of FMD. This study has been done initially with FMD known serotypes (O, A, Asia1) already in use at animal health.Viral RNAs were extracted using R Neasy Minikit (...
Nancy R. Howell
...n and redemption. In critique, I ask two questions: (1) What does Peters’ Cosmic History contribute to addressing the issue of particularity? (2) In what ways does Peters lead us to deeper thinking about the nature of God? Surely, reflection on these two questions will show that Peters has generated a fruitful method for and approach to Cosmic History.
...
George L. Murphy
... history. But, in my critique, I contend that it is God that provides this meaning, not we human beings. Instead of just an anthropic principle, I appeal to a theanthropic one. It is God who created the cosmos so that flesh would evolve for the Word to become flesh, and to unite all things in that incarnate Word.
...
Ted Peters
...er responses to each critique.
...

Ronaq Zaman*, Shahid Ali and Inam Ullah 

...ulti-stage sampling technique through well-structure interview schedule. For the estimation of Stochastic frontier tans-log production function, maximum likelihood estimation technique was used. To ensure the abnormality of data set Chow test and Dummy variable test was employed for any possible structure break across the broiler farms. Results indicated that there were significant structural breaks across the sampled farms....

Saima Akhtar Qureshi1 and Asim Anwar2*, Ather Maqsood Ahmed

...in Pakistan. In IPM technique natural parasites and predators are used to check pest growth instead of pesticides which reduces ecological and health damage. Pesticides are used at the last resort in IMP method so it reduces the cost of production and increases the farmers’ profit. However, concerns about production may hinder widespread adoption of this technology by farmers. The aim of the study is to evaluate economic feasibility of IPM method in Punj...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
...convenient sampling technique. Biochemical examination was performed to isolate S. aureus that further were tested Penicillin, Glycopeptide, and Cephalosporin classes of antibiotics for confirmation of MDR isolates using disk diffusion method. MDR were tested in vitro for response against various antibiotics. A questionnaire was filled for estimation of association of assumed risk factors with prevalence. Nonparametric tests at 5% probability wer...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...sing cheap and safe techniques and making it stable for long period of time in food products as well as in extracted form by using preservatives and antioxidants like citric acid and determining its usefulness by applying on food products. Natural edible color was extracted from T. erecta gave lemon yellow color shade which when applied on candies increased the attractiveness. The spectrophotometric analysis at 474nm of extracted color both in crude for...

Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar

...obability purposive technique of sampling was used. Diagnostic laparoscopy was conducted to make a diagnosis of endometriosis. Females were divided into two groups. Females in group A received treatment with Letrozole tablets (2.5 mg/day) and in group B received Danazol tablets (600 mg/day) for 3 months. Pelvic pain was assessed using VAS score and the mean decrease in pain score was assessed between two groups using independent sample student t-test. Results ...

Abida Sajid* and Aqsam Sajid 

...onsecutive sampling technique. Data was collected on a pre-designed questionnaire. A total of 3855 deliveries during study period in which 1318 were primigravida. Mean age in study group was 15 to 24 years. We observed mild anemia in 46% of woman, moderate anemia in 41% and severe anemia in 13% in young primigravida. Preterm delivery was noted in 25% of the females and low birth weight babies were noted by 33% in teenage and young primigravida. Statistically s...

 Aneela Afzal, Tanvir Ali, Munir Ahmad, Humera Amin and Samina Saki*

...age random sampling technique was used to collect the data from 480 respondents from three tehsils of the district. The results show that a vast majority of women were illiterate, having annual family income less than one lac, living in joint family system and having small land farms. A large proportion of the respondents reported that they remained engaged in cleaning of seeds, sowing, harvesting, weeding, etc. for corn, rice, wheat, sugarcane, and vegetables...

 Shazia Erum, Rashid Anwar and Shahid Masood*

...asuri methi acquired a unique status as a G.I of Kasur. Cluster analysis (UPGMA) of 28 genotypes including both methi and methray from various agro ecological zones of Pakistan were interlinked to some extent however Kasuri methi make their identity by standing alone among tested genotypes on the dendrogram, due to the geographical environment of Kasur in Punjab. In this connection ‘Benefit sharing’ mechanism can be accelerated by escalating farmer...

 Sarfraz Ahmad, Shaista Koukab, Nayyar Razzaq, Muhammad Islam, Aneel Rose*, and Muhammad Aslam**

...ges, oil extraction techniques, mechanized flower picking, drying/storage techniques and cultivation practices in the existing cropping patterns.

...

Muhammad Athar Khan1, Muhammad Zahid Latif2*, Syed Amir Gilani3 and Ifrah Bukhari4 

...e stage probability technique was used for sampling. In the first stage one union council was selected from each district randomly. In the second stage, 25 subjects involved in rice cultivation from the last 10 years were selected from each union council. Similarly 25 subjects who never worked in the rice paddy field were also randomly selected from each union council as controls. The serum sample of each subject was tested against each of the five antigens ag...

Halima Shoukat1*, Hafiz Sheraz Arshad2, Faiza Sharif1, Arooj Fatima1 and Filza Shoukat

...e joint. Stretching techniques are used to treat muscle tightness. In clinical settings, different stretch holding times are used to treat hamstring tightness. The purpose of the study was to determine effects of different static stretch holding-times on hamstring muscles flexibility and their impacts on improving range of motion (ROM) of knee extension in adults. This experimental work was conducted on students of University of Lahore from December 2013 to Ma...
Abul Hassan Faiz*, Fakhar-i-Abbas and Lariab Zahra Faiz
...lying line transect technique in eight types of soil (sand, loamy sand, sandy loam, sandy clay loam, loam, clay loam, silt loam, silty clay loam) to determine six soil properties (plant biomass, saturation, electrical conductivity, pH, organic matter, phosphorus and potassium) in Pothwar Plateau (250 km long and 100 km wide). We compared 216 soil samples (mound soil and undisturbed soil), to collect data on percentage water saturation, electrical conductivity ...

Imtiaz Hussain*, Hassnain Shah, M. Azeem Khan, Waqar Akhtar**, Abdul Majid*** and M. Yaqub Mujahid*

Corresponding author:izhussain@yahoo.com

PRODUCTIVITY IN RICE-WHEAT CROP ROTATION OF PUNJAB: AN APPLICATION OF TYPICAL FARM METHODOLOGY
...rent wheat planting techniques in relation with residue management. These techniques may include farmer practice (partial burning, land preparation and broadcast); partial burning zero tillage ; Farm Machinary Institute seeder (planting with in the residue). 

...

 Shazia Erum*, Muhammad Naeemullah and Shahid Masood**

Corresponding author: shazia_rm@yahoo.com

PHENOTYPIC VARIATION AMONG MENTHA SPP.
...n latest scientific techniques. Like other herbs Mentha have diverse aromatic properties that can be explored for utilization in the industry and this information will be helpful for exploitation and development of natural agricultural resources on scientific grounds.

...

  Farhat Ullah Khan, Nowshad Khan and Zaibunnisa Abdullah*

Corresponding author: farhatkhan7@gmail.com (This is part of Ph.D. work of senior author)

IMPACT OFKINNOW (MANDARIN) YIELD LOSSES ON SOCIOECONOMIC CONDITIONS OF THE FARMING COMMUNITY IN PUNJAB, PAKISTAN
...tage and chi-square techniques were used for analysis. According to analysis 91.5% respondents reported that yield losses of kinnow fruits have negative impact on socio-economic conditions of the majority respondents of the project area. Chi-square test also shows negative link of the kinnow fruits and socio-economic uplift of the farming community in the project area. Serious attention on scientific basis to yield losses through awareness and training of exte...

 Muhammad Arifullah, Muhammad Munir*, Abid Mahmood**, Saifullah Khan Ajmal and Ghulam Shabbir*

COMBINING ABILITY ANALYSIS OF SOME YIELD ATTRIBUTES IN INDIAN MUSTARD (BRASSICA JUNCEA L.)
...cept plant height and siliqua length. UCD-8/4, KJ-119 and BRS-2 were good general combiners for yield related traits. A cross BRS-2 × UCD-8/4 showed best desired SCA for number of primary branches and siliquae per plant. S-9 × Canola Raya for siliqua length, Canola Raya × UCD-8/4 for number of seeds per siliqua, KJ- 119 × BRS-2, B...

 Muhammad Zubair Anwar, M. Azeem Khan, Ikram Saeed, Akhtar Ali*, Shafique Zahid and Abdul Majid**

SMALL FARMERS PERCEPTIONS REGARDING IMPROVED FODDER AND FORAGE VARIETIES: RESULTS OF PARTICIPATORY ON FARM RESEARCH
...fodder preservation techniques for lean period. To resolve this issue improved fodder varieties and forage species were introduced. About 52 % women were of the view that in rabi season 50% grain yield was increased and 44% said that the increase in dry stalk was about 50%. While in kharif 72% respondents pointed out 55% increase in grain yield and similarly majority of the farmers (68%) pointed out 50% increase in dry stalk yield. Majority of the respondents ...

 Amir Khatam*, Sher Muhammad, and Ijaz Ashraf**

ROLE OF INDIVIDUAL CONTACT METHODS IN DISSEMINATION OF AGRICULTURAL TECHNOLOGIES
...hedule using survey technique. The results show that majority of the farmer respondents is aware of the farm visits (66.43%), demonstrations being conducted (62.50%) and home visits made by the extension staff (60.71%) as extension methods for dissemination of agricultural technologies among the farming community, whereas only 31.78% and 22.85% of the farmer respondents are aware of the office call and telephone calls, respectively, for diffusion of informatio...

 Amir Khatam*, Sher Muhammad and Ijaz Ashraf** 

ROLE OF FARMER FIELD SCHOOLS IN ENHANCING SKILLS OF FARMING COMMUNITY IN KHYBER PAKHTUNKHWA, PAKISTAN
... practicing various techniques by themselves. Considering therefore, the importance of this approach, the study was conducted in 2011 to examine the role of FFS in enhancing skills of farming community in the central region of Khyber Pakhtunkhwa, Pakistan. The data were collected through survey method on various aspects from 280 randomly selected farmer respondents. The data collected were analyzed by applying simple descriptive statistics showing means, score...

 Sara Khalid*, Khalid Mahmood Qureshi*, Ishfaq Ahmad Hafiz*, Khalid Saifullah Khan* and Usman Shaukat Qureshi*

EFFECT OF ORGANIC AMENDMENTS ON VEGETATIVE GROWTH, FRUIT AND YIELD QUALITY OF STRAWBERRY
...rganic agricultural techniques are utilized globally to protect our environment and prevent health issues resulting from pesticides and hazardous chemicals. In this regard, studies were conducted using six different organic amendments on strawberry (Fragaria ananassa Duch.) cv. Chandler which included T = planting 1 media -1 (soil + silt + farm yard manure); T = planting media + 400 mgl humic 2 -1 acid; T = planting media + 200 g kg leaf manure; T = planting m...

 Abid Hussain*, Khalid Mahmood Aujla* and Sonila Hassan**

PRODUCTION AND MARKETING OF CAMEL PRODUCTS IN SEMIDESERT AND DESERT AREAS OF PAKISTAN
...ple random sampling technique in April, 2011 from 220 camel farmers and 17 market intermediaries. It is found that both camel farmers and market intermediaries were less educated. It is observed that markets for camel milk, meat, hides and hair are less established in semi desert and desert areas of the country. Mean production of milk per farm household was 5.4 and 6.5 liters per day in summer and winter seasons, respectively; however, none of the surveyed fa...

 Muhammad Shahzad*, Akhter Ali**, Abdul Hayee Qureshi**, Naveed Jehan*, Imran Ullah* and Majid Khan*

ASSESSMENT OF POST-HARVEST LOSSES OF PLUM IN SWAT, PAKISTAN
...ied random sampling technique was used for the collection of data from different categories of respondents and tehsils. Post harvest losses were estimated through percentage method. The most important reasons were poor harvesting methods, over maturity, insufficient cold storage facility, poor grading, low-quality packaging materials and poor infrastructure. Total post harvest losses in the marketing channel of the plum were 21.51% of which 5.12% occurred at f...

 Adnan Umair*, Safdar Ali**, Muhammad Sarwar***, Kashif Bashir**, Muhammad Javed Tareen**** and Muhammad Asghar Malik*****

ASSESSMENT OF SOME PRIMING TECHNIQUES IN MUNGBEAN (VIGNA RADIATA) : A GREEN HOUSE STUDY
...  Priming technique used for achieving better crop stand, in which seeds are partially hydrated to a point where germination process begin but radical emergence does not occur. A pot experiment was conducted in a green house. The seeds were invigorated by traditional soaking (hydropriming), osmo-conditioning using phosphorous (KH PO ; 2 4 -1 200 mM and 400 mM), mannitol (20 and 40 gl ), polyethylene glycol (50 and -1 -1 100 gl ), sodium molybdate dih...

 Abid Hussain*, Khalid Mahmood Aujla* and Sonila Hassan**

ECONOMICS OF COW MILK PRODUCTION IN SINDH AND AZAD JAMMU & KASHMIR
...ple random sampling technique. Shares of different feed resources in total feeding cost have been determined and cost-benefit analysis of milk production by ecological zones was also conducted. Wide variations in share of feed sources in total feeding cost per annum, milk productivity and profitability of main cow breeds across selected areas have been observed. Concentrate feeding is the main cost item for cow milk production in irrigated areas of Sindh and c...

Tanzila Kazmi*, Abid Ghafoor Chaudhry*, Aftab Ahmed** and Shaheer Ellahi Khan**

FARMERS BELIEFS ABOUT INDIGENOUS FARMING PRACTICES AND SUSTAINABLE AGRICULTURAL DEVELOPMENT
...ing the statistical technique in which the sample of 187 farmers were drawn out of the population of 3680, to cultivate insight and beliefs regarding the indigenous farming practices and how it is related with sustainable agricultural development. During the study a mixture of qualitative techniques including interview guide(s), focus group discussions (FGDs) and case study were used. The study revealed that indigenous knowl...

 Nazir Ahmad*, Sultan Ayaz*, Sumaira Shams**, Karimullah*** and Riaz Ahmad****

PREVALENCE AND MORPHOLOGY OF HELMINTH PARASITES OF FISH FROM RIVER SWAT, KHYBER PAKHTUNKHWA
...through microscopic techniques. Overall prevalence of the fish parasites was 58% (145/250). Among these Schizothorax plageostomus fish 93.04% (107/115), Schizothorax labiatus 61.11% (33/54), Salmo trutta fario 17.85% (05/28), Gara gotyla 0% (0/09), Rita rita 0% (0/25) and Oncorhynchus mykiss were 0% ( 0/19). The intensity of the parasite varied from 1% to 9.2%. Among them high intensity was noted in Rhabdochona schizothoracis (9.2%) and Schizothorax labiatus.<...

 Muhammad Ali*, Ghulam Mustafa Sajid**, M. Faisal Anwar Malik*** and Kalimullah****

ESTABLISHMENT OF IN VITRO CULTURE OF GRAPES
...ough tissue culture technique. These explants were collected from gene bank of Institute of Agricultural Biotechnology and Genetic Resources (IABGR), National Agricultural Research Centre (NARC), Islamabad, Pakistan. Fifteen accessions of grapes were surface sterilized and tested on 75% MS media for germination and initial growth parameters. Accession No. 020017 (Dakh-1) exhibited highest viability (100%), shoot length (4.12 cm) -1 and nodes plant (3.8). Moreo...

 Samiullah*, Mehmood Shah*, Kalim Ullah**, Rehmat Ullah*** and Ibrarullah****

PROFITABILITY OF WHEAT PRODUCTION IN DERA ISMAIL KHAN
...zed. Farm budgeting technique for estimating cost, gross return and profitability was applied. The analysis of data revealed that the average cost per acre was Rs. 24716.32. Similarly average production of wheat (output) was estimated to -1 -1 be 1618.40 kg acre . Thus the gross return was Rs. 45315.39 acre , whereas the net return (profit) from wheat production was Rs. 20599.07 -1 acre . It is thus concluded that profit is under the direct positive influence ...

 Naheed Zahra*, Hassnain Shah*, M. Azeem Khan* and M. Zubair Anwar* 

LIVELIHOOD ASSETS AND POVERTY NEXUS: A CASE STUDY FROM RAINFED POTHWAR AREA OF PAKISTAN
... poverty score card technique. The poverty status of household was highly correlated with the livelihood sources (P<0.01), water availability for irrigation(P<0.10) and tenancy status (P<0.05). The results regarding poverty revealed that 2.50% of the sampled households were chronically poor, 5.0% transitory poor, 37.5% transitory vulnerable, 32.5% transitory non- poor and 22.5% was non-poor. Most of the sampled households fall along the transition lin...

 Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari* 

ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...be used as a marker technique to ascertain the hybrid nature of offsprings and to evaluate parental lines in terms of genetic diversity.

...

 Muhammad Akhter*, Muhammad Azhar Ali**, Zulqarnain Haider* and Shahzad Muzammil***

PHYSICO-CHEMICAL CHANGES IN GRAINS OF SOME ADVANCE LINES/ VARIETIES OF RICE (ORYZA SATIVA L.) AFTER PARBOILING
...dardized parboiling technique for improving nutritional, milling and cooking qualities of these lines/varieties. For this purpose, white milled and parboiled rice samples of ten lines/varieties were analyzed for nutritional quality parameters such as ash, dry matter, crude fat, crude protein, crude fiber, vitamin B6; milling quality parameters such as total milling recovery, head rice recovery, ratio of broken grains and cooking quality parameters such as curl...

 Khurram Nawaz Saddozai*, Umme Rubab* and Abass Ullah Jan* 

STOCHASTIC FRONTIER ANALYSIS OF MAIZE FARMERS IN AZAD JAMMU AND KASHMIR, PAKISTAN
...allocation sampling technique was adopted to collect primary data from 80 sampled respondents in 2013-14. The maximum likelihood estimates of major inputs showed that seed, tractor hours, FYM and labor days have contributed significantly to increase the maize yield. However, the DAP and urea have shown no effect on maize yield. The mean technical efficiency was estimated at 83%, implying that the farmers can still enhance their technical efficiency by 11% with...

 Khurram Nawaz Saddozai*, Muhammad Nasrullah* and Noor P. Khan** 

STOCHASTIC FRONTIER PRODUCTION ANALYSIS OF TOBACCO GROWERS IN DISTRICT MARDAN, PAKISTAN
...ulti-stage sampling technique was utilized to obtain the desired sample size of 120 tobacco growers. The major findings of stochastic production frontier analysis indicate that all variables were statistically significant and have portrayed positive contribution to tobacco production except fertilizer which was found significant but has revealed inverse relation with tobacco production. The mean technical efficiency was estimated at 0.85 depicting that tobacco...

 S. Talat Gilani*, Shahid Hameed* and Hussain Shah* 

OCCURANCE AND DISTRIBUTION OF POTY VIRUSES INFECTING GARLIC IN PAKISTAN
...l testing DAS-ELISA technique was used to test the samples collected from the farmer fields. Based on the DAS-ELISA poty viruses OYDV and LYSV were detected from both provinces although the percentage incidence varied from location to location. Few areas of district Punjab were found free of LYSV but OYDV was prevalent in all locations irrespective of the varieties cultivated. Maximum disease incidence was detected in Swabi (KPK) where OYDV was 90% and LYSV wa...

 Hina Fatima** and Bushra Yasmin**

EFFICIENCY AND PRODUCTIVITY ANALYSIS OF PAKISTAN'S FARM SECTOR: A META-ANALYSIS*
...ric, non-parametric techniques, functional forms, data types, production technology dummies and variable size parameter. The analysis revealed the overall technical efficiency of Pakistan's farm sector as 73%. The results from meta-analysis clearly indicated that farm sector in Pakistan is way behind its optimum potential level. Hence there is sufficient room for improvement. To this end, government should be well-designed to evaluate the misdirected growth po...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
... of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly isolated H3N2 viruses from dogs in China and had the same evolutionary branching. The result provided a foundation for further studies on biological characteristics of CIV.
...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2 and Zafarullah Baloch3 

.... Snowball sampling technique was used to select 90 respondents. The data revealed that average age of the farmers was 53.9 years with education from secondary to graduate level. The results of the study showed that many of the farmers on average have 10 acres of land under date palm cultivation with more than 57 date palm trees per acre on average. It is also observed that respondents generally get 76 kg of dates on average per tree. Many of the farmers use R...

Jesse M. Smith

...is by employing the techniques of ethnographic content analysis consistent with the inductive logic of a constructionist grounded theory. Combing elements of rhetorical theory with a sociological view of religious movement and marketplace, I identify the major themes and evolving narrative of this American religion in relation to secular historical and cultural conditions. The data suggest the rhetorical strategies revealed in Mormon leaders’ discourse o...

Irfan Ullah, Shahid Ali*, Muhammad Fayaz and Abbas Ullah Jan 

...age random sampling technique. Stochastic frontier cost function of Cobb- Douglas type was used for the estimation of allocative efficiency. The function was estimated using Maximum Likelihood estimation technique. Results revealed that the mean allocative efficiency of broiler farms was 0.84; implies that an average farm could have used 16% less inputs for given level of output if inputs were allocated efficiently. The esti...
Aqsa Gulzar1, Tariq Mahmud1,*, Rubina Munir2 and Asma Anjum3
...rious spectroscopic techniques namely FTIR, proton and carbon-13 NMR, Mass spectrometry and elemental analysis. The results were in great consistency with the structures of the synthesized compounds and confirmed the formation of the targeted compounds. It was found that the compound 4a was potentially anti-inflammatory active while the compound 4b was found more active anti-cancerous agent as compared to 4a.
...
Grace C. Onyishi, Ifeanyi Oscar N. Aguzie, Christopher D. Nwani*, R.N.N. Obiezue and I.C. Okoye
...ia Medical Centre and Sadiq Hospital were examined for malaria prevalence and intensity from January to July 2015. Indoor biting Anopheles mosquitoes were collected by standard procedures within the study duration. Meteorological data on relative humidity and temperature was obtained from Centre for Basic Space Science, Nsukka, Enugu State. Overall recorded malaria prevalence at the hospitals from January to July was 72.8%. Malaria prevalence was highes...
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...t that reproduction technique may be an important factor affecting some layer performances, egg quality characteristics and welfare in local poultry breeds. These findings are expected to provide a theoretical guide to choice of breeding methods for poultry enterprises.
...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...emia Centre, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad and the Pakistan Thalassaemia Centre, Pakistan Baitul Mal, Islamabad, Pakistan. Data were obtained by clinical testing of 1,440 ß thalassaemia major patients visiting for blood transfusions at regular intervals. The confirmatory screening for HBV and HCV was performed through Chemiluminescent Immunoassay (CLIA). Of the total 1,440 patients studied, 930 (64.6%) were males and 510 (35.4...
Waqas Ali1,*, Arshad Javid1, Syed Mohsin Bhukhari1, Ali Hussain1, Syed Makhdoom Hussain2, Hira Rafique1
... Different sampling techniques are applied during herpetofaunal surveys and some of them might be expensive, time intensive and their effectiveness in different regions of the world are still debatable. Moreover, data recorded from inappropriate designed surveys are not suitable for statistical analysis and provide inappropriate picture regarding distribution, abundance and status of target species. Current review inspects different sampling techn
Jehangir Khan1,*, Rasheedullah1, Bakht Tarin Khan2, Ayaz Ahmad3, Muhammad Salman4 and Khalid Khan5
...rbant Assay (ELISA) techniques were applied for the presence of anti-HCV, while RT-PCR was used for active HCV infection. Our study reports an overall prevalence of HCV among 131 (14.55%), 103 (11.44%), and 94 (10.4%) by ICT, ELISA, and RT-PCR techniques in individuals belonging to 5 different age groups. The RT-PCR based results showed 53% HCV infection rate in samples from Tehsil Puran, 23% in Alpuri and 18% in Besham. The...

Nihal Ahmad Hadi, Shahid Ali* and Umer Wahid

 

...ulti-stage sampling technique was used to collect data from 150 broiler farmers. A well-structured interview schedule was used with both close and open-ended questions. Data from broiler farmers were collected during April and May 2016. Maximum Likelihood Estimation (MLE) technique was used for estimation of stochastic frontier Cobb-Douglas type production function. Results indicated the mean technical efficiency of 0.89 wit...

Shahid Ali1* and Salamat Ali2  

...Multistage sampling technique was applied for selection of sampled respondents. 120 farmers, 60 farmers of open shed and 60 farmers of EC shed were interviewed during 2014. Dummy variable regression was applied for estimation and comparison of costs and revenues of open shed and EC shed broiler farms. Results revealed that there was statistically significant difference between the cost of production and net revenue of open shed and EC shed farms. Cost of produ...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...ted egg inoculation technique. Moreover, out of 507 samples, 479 serum samples were scrutinized by enzyme linked immunosorbent essay (ELISA). Sero-prevalence of AIV among different species of wild birds was as follows; peacock (n=35; 14%), duck (n=5; 2%), migratory water fowl (n=3; 1%), pheasant (n=2; 0.8%), grey leg goose (n=1; 0.4%), turkey (n=1; 0.4%), eagle (n=1; 0.4%) and crane (n=1; 0.4%), for domesticated bird species sero-prevalence was; broiler (n= 15...

Safdar Ali1*, Obaidullah Shafique1, Tariq Mahmood2, Muhammad Amir Hanif1, Ijaz Ahmed3 and Bashir Ahmad Khan

...on. Precise farming techniques can promote crop production without damaging soil and water, reducing losses due to leaching of nitrogen and enhancing long lasting incorporation of nutrients by micro-organisms present in the soil. Another major breakthrough of nanotechnology for improvement in agriculture production is the development of insect-resistant varieties by DNA transfer in the plants or nanoparticle-mediated gene. Biofuel production from biomass is es...
Arshad Mahmood Malik1, Khalid Mahmood Mughal2, Sarfraz Ahmed Mian2 and Atta Ullah Khan2
...rket Pvt. Ltd which is unique entity involved in commercial production of hydroponic products in Pakistan. Data on various production and economic variables was collected from FMP during 2009 to 2013. Variable of the study were area, area allocation decisions of farmer, customer structure, markets, costs involved in production process and revenue earned from selling of the produce in both local and in export market. Yield of hydroponic tomatoes was measured in...
Yakup Erdal Erturk1, Adem Aksoy2 and Mohammad Masood Tariq3,*
...rametric regression technique, was used to develop a respectable prediction equation that can denote interaction effects of influential predictors in the definition of the influential factors on AFFLW as an output variable for male and female crossbred cattle. Several predictors in the current survey were province (Erzurum, Igdir, Kars and Agri), farmer age, educational level (illiterate, primary school, secondary school, high school, and college), social secu...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...C) and high performance liquid chromatography (HPLC-UV) to get the indication of the identity of the compounds responsible for antimicrobial activity and biofilm dispersion. Overall the results indicated that natural extracts from these Streptomyces strains and their active components can be exploited for the preparation of anti-caries products like mouth washes and tooth pastes etc.
...
Nadia Saeed1,Mian Sayed Khan2, Habib Ahmad3, Muhammad Abdullah4 and Muzafar Shah5,*
...ing Bearmann funnel technique. After Quantitative analysis of both treated and untreated trees, it was observed that treated trees samples showed decline in nematode number as compared to untreated trees. Organic amendments effectively controlled the nematode population. Among all applied organic amendments castor oil cakes and cow dung were found more effective and saw dust was least effective. The studies showed that organic amendments were significantly eff...
Farrukh Jamil*, Samra Raheel and Haroon Rasheed
...or.
...
Tengfei Lu1, Wenhua Pei1, Shuang Zhang2, Yangnan Wu1, Fenghao Chen2, Xiao Han2 and Weijun Guan1,*
... immunofluorescence technique. The results showed that they were positive in AMSCs. The growth of different passages cells typically appeared in S curve. Furthermore, AMSCs successfully differentiated into osteoblasts, adipocytes, and hepatocyte-like cells after procedure induction. The results showed that the AMSCs obtained from sheep demonstrated the features of multipotent stem cells. Therefore, AMSCs could be a good option for transplantation for treatment...

Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair

...he MDV virulence and a unique distortion in the Proline repeats (Proline-to-Alanine) at position 176 in the Egyptian MDV strains. The Phylogenetic analysis grouped the eight analysed sequences with the previously investigated Meq from Egypt (2011-2013) together with the very virulent European and Chinese MDV isolates. The latter confirmed the geographical structuring of the Egyptian MDV strains together with the Eurasian origin viruses in an independent taxono...
Muhammad Anwar-ul-Haq1, Amer Jamal Hashmat1, Ejazul Islam1, Muhammad Afzal1, Abid Mahmood2, Muhammad Ibrahim2,*, Muhammad Nawaz3,*, Shahid Nadeem4 and Qaiser Mahmood Khan1
...ing electrochemical techniques on pilot scale (70 L). One of the major problems encountered in the textile industry is the production of large volumes of highly colored wastewater. The scope of this new electrochemical treatment is a significant advance to the existing technologies. There were four different treatments employed as; T1: wastewater, T2: wastewater with 0.1% electrolyte, T3: diluted wastewater (50% tap water), and T4: diluted wastewater (50% tap ...
Musrrat Fatima, Muhammad Saad Khan, Hamid Rashid, Asim Mehmood, Sumaira Kanwal, Muhammad Asif Rasheed and Farrukh Jamil*
...pharmacoinformatics techniques to identify potential inhibitors against ZIKV protease NS2B-NS3, which has a well-known role in the viral replication. By using three dimensional structure of the NS2B-NS3, an allosteric site has been identified in the protein. Moreover, the identified site is used for structure-based virtual screening. After screening 100,000 compounds, 14 compounds are selected, which fulfilled different already established drug likeliness para...
Yakup Erdal Ertürk
...parametric analysis technique was preferred in the description of the influential factors and their interaction effects for each gender. Some probable factors such as age, province, education level, experience, social security, lands, and the reason at performing animal production were recorded on breeders. Also, first fattening weight and fattening period of the beef cattle were recorded. It was determined that predictive models based upon MARS algorithm expl...
Nina Bao1,*, Yongguang Yin2 and Panpan Wang3
... research the effect of liquid-to-solid ratio, standing time and centrifugation time on the extraction of crude cerebroside. The optimal extraction conditions for the cerebroside as follows: liquid-to-solid ratio 15, standing time 45 min, and centrifugation time 6 min. The extracted cerebroside was purified and identified by using silica gel column and TLC, respectively. IR analysis showed that the extraction has hydrox...
Zhuanxia Chen1, Ningping Tao1,*, Xiao Qin1 and Dayong Liu2
...d electronic tongue technique. The characteristic of the taste substances were tested, such as free amino acids, nucleotides, trimethylamine oxide and betaine. Results showed that umami was the most prominent flavor. Compared with liver, IMP is the main flavor nucleotide in meat. However, the contents of the umami amino acids and betaine in liver are much higher than that of meat. Additionally, trimethylamine oxide is mainly found in the meat. After steaming, ...
Nasreen Ishaq1, Arif Rasheed Malik2, Zameer Ahmad1, Saad Ehsan Ullah3
...ons, however, these techniques are expensive and not readily available necessitating additional techniques for identification. One of such novel approach is cheiloscopy i.e. study of lip print patterns. 
Methodology: In order to investigate the lip prints-based identification, a study was conducted in the Shaikh Khalifa Bin Zayed Al-Nahyan Medical College, Lahore. A total of 125 female and 125 male st...
Doğan Türkyılmaz1,*, Selçuk Özyürek2,Nurinisa Esenbuğa1 and Mustafa Yaprak1
...ng is pear-shaped and oblique teat (Type III). So this result can help us for selection of milking ability in Morkaraman and Tuj breeds.
...
Muhammad Kashif Munir1*, Sana Rehman1, Rizwan Iqbal1, Muhammad Saqib Saeed2, Muhammad Aasim1
...t modern and latest technique, particularly in smear negative patients, helps rapid detection of TB and rifampicin resistance, which facilitates prompt diagnosis of multidrug resistant TB. These results propose that effective treatment can be initiated at an early stage, which will greatly help in reducing multidrug resistant TB.
...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
Faisal Masood*1, Ranjit Kumar Sah2 and Ahmad Humayun Sarfaraz3
... fracture by Faisal Technique.
Methods: A total of 10 cases of volar Barton’s fracture fulfilling our inclusion and exclusion criteria were included in our study from August 2015 to August 2016. These cases, presented at our department, were managed with closed reduction under image intensifier by dorsiflexing the wrist and reducing the fragment by ligamentotaxis and percutaneous pinning in anti-glide fashion from dorsal proximal (inta...
Mumtaz Akhtar, Iffat Naheed, Khadija Waheed* and Amna Khanum
... purposive sampling techniques were used. A total of 220patients undergoing normal vaginal delivery at term after administration of per rectal misoprostol were enrolled with 95% confidence interval (C.I). The error margin was 5% and the prevalence of postpartum haemorrhage was 16.5%. Data was entered and analyzed through SPSS 17. In this study, the mean age of study participants was found to be 29.89±5.89 years while the mean gestational age was 38.86&p...
Xia Lin Zheng1, Zhen Hua Xian1, Zhen De Yang2, Xiu Hao Yang3 and Wen Lu1,*
...Castanopsis hystrix Miq. (Fagales: Fagaceae) in Pubei County of Guangxi, China were investigated from December 2012 to December 2013. Jaapiella sp. damaged leaves, while Contarinia sp. damaged stems and leafstalks. The damage ratio and the index of Jaapiella sp. in December 2012, March, July and December 2013 were 9.7±2.0%, 14.8±1.9%, 24.1±1.5%, 12.3±0.6% and 25.6%, 31.3%, 35.6%, 26.2%, respectively, while t...

G. Venkatesan* and Amit Kumar  

...ls and their products. Unique and specific virulence genes located at termini of ORFV genome with their encoded products are attributed to host immune evasion, short-term immunity both in natural infection post-vaccination in target species and repeated host susceptibility. However, these genes are variable among ORFV isolates/strains as they are not located in central part of genome and some of them are either virus specific namely Viral interferon resistant ...
Wang-Dui Basang1, Tian-Wu An2, Luo-Bu Danjiu3, Yan-Bin Zhu1, Shi-Cheng He3, Xiao-Lin Luo2, Wei-Wei Ni4, Xiao Wang4, Shu-Zhu Cheng4, Jian Wang4 and Guang-Xin E4,*
...based on the number of unique haplotypes within each population; however, they have not heavily and significantly diverged because all the populations share the most high-frequency haplotypes. Therefore, this study not only shows that the different ecotype populations of the Naqu yak carry high genetic diversity but also indicates that frequent genetic material exchanges have led to smaller differences in genetic divergence between different ecotypes.
...

Raphael Olanrewaju Babatunde, Adeyemi Esther Omoniwa and Miriam Ukemenam 

...Nigeria. A sampling technique comprising of three stages was used to randomly select 200 farming households in the study area. Data were collected using questionnaire and were analyzed using the Gini-coefficient and the Ordinary Least Square method. The result showed an overall gini-coefficient for gender inequality in schooling of 0.35, with the value for boys and girls at 0.35 and 0.49 respectively. Mother’s education, age of the household heads, marit...

Sadiqa Begum1, Murad Khan2* and Noor Pao Khan

.... Budget allocation technique is used for calculation of cost, revenue and profit and dummy approach is used for comparative analysis while multiple regression model is used to estimate the relationship between inputs and output of wheat. Per acre yield of wheat production in district Mardan was 1809.032 kgs. while in district Peshawar it was 2143.230 kgs. Total cost of wheat per acre in district Peshawar was more than that of district Mardan. The net revenue ...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...hain reaction (PCR) technique was used for the molecular detection of NPV gene from native NPV diseased insect. Multiple sequence alignment and phylogenetic analysis were performed to compare SlNPV- FSD15 based on Lef-8 with other Lef-8genes sequences clearly showed that our SlNPV-FSD15 isolate belongs to Spodoptera litura associated NPVs. The biological activities of this NPV isolates were investigated under laboratory condition. T...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2 and Mujahid Iqbal3  

...systematic sampling technique using the available list of the farmers provided by Agricultural Extension Department. The respondents were than randomly assigned into two groups, one group was comprised of experimental group (to whom a set of advisory instructions were sent by mobile phone) and other was control (face-to-face). Two- population independent t-test was applied for comparison of the two methods of instructions. The results of the study revealed tha...
Rida Zainab1, Sana Elahi1, Afshan Kaleem2,*, Daniel C. Hoessli3, Mehwish Iqtedar2, Roheena Abdullah2, Faiza Saleem2, Shanza Khan1, Anusha Ijaz1, Shagufta Naz2 and Abdul R. Shakoori4,5,*
...ased drug designing technique, has emerged to develop more efficient drugs. One of the computational methods becoming increasingly popular to develop new drugs is relying on Pharmacophores. This method was utilized to develop pharmacophore models of Akt2 inhibitors and β2-Adrenoceptor agonists. A pharmacophore model is proposed, using fourteen second generation and one first generation antipsychotic drugs for Schizophrenia that are effective against both ...

Aftab Khan, Shahid Ali, Syed Attaullah Shah and Muhammad Fayaz 

...multistage sampling technique. Primary data on net revenue from maize crop was collected through an interview schedule and secondary data on temperature and precipitation was collected from Regional Metrological Department, Peshawar. Cross sectional Ricardian Model was used to estimate the effect of temperature and precipitation on net revenue. Results of the study revealed that temperature has positive effect and temperature square has negative effect on net ...
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
..., -80 oC and liquid nitrogen freezing at -196°C) are used by different laboratories. In this study, the storage stability of five bacteriophages, JHP, RLP, RSP, SaPL, and IttPL, at distinct temperatures for complete one year is described. Our results indicated that storage of bacteriophages at 4oC was most appropriate, followed by storage at -20oC and -80oC as no drop in viral titer was witnesses in bacteriophage...

Qaisar Mehmood1*, Muhammad Riaz2, Maqbol Hussain Sail1 and Muhammad Moeen1 

...nalysis of variance techniques are used which show that variety, sowing mode, nitrogen fertilizer and phosphorous fertilizer have significant effect on the wheat yield. Irrigation mode and weedisides spray also has much impact on the wheat yield. 

...

Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...phate precipitation technique and kinetic characterization was also performed against different pH and temperature. CAT activity was measured spectrophotometrically at A240 nm. Results showed that highest CAT activity was noted from the crude extract of control fish gills (140.66±0.71UmL-1) as compared to DM+END exposed fish (99.23±0.71UmL-1). After desalted the CAT activity in gills of both control and insecticides exposed fish was calculated as...
Nimra Naseer, Adeela Fatima and Imran Sajid*
...nified the presence of unique and diverse metabolic compounds. The study predicts that the seactinomycetes strains are a potential resource to cope up with evolving multi drug resistant pathogens. Furthermore, these secondary metabolites can be identified by comparative studies and these actinomycetes can be grown on larger scale to obtain high yield of potentially useful secondary metabolites.
...
Merica Slišković1, Meta Povž2, Marina Piria3,*, Goran Jakšić4, Ana Gracanin5 andGorana Jelić Mrčelić1
...using sport fishing techniques. The age, condition, length-weight relationship (LWR), 20 morphometric and four meristic traits, were analysed. Fulton condition coefficient and LWR value indicated that sichel specimens’ were well adapted to the environmental conditions. Positive allometry in both males and females were observed (W = 0.004 L3.119). This represents the first LWR dataset for sichel. The morphometric and meristic trai...

Muhammad Israr1*, Saeed Ullah1, Shakeel Ahmad2, Asif Yaseen3, Urooba Pervaiz4 and Nafees Ahmad5 

...ate random sampling techniques form 90 sampled farmers through face to face interview survey method by filling a questionnaire. Farmer’s perceptions on agriculture LD were measured through the extent of severity scale in terms of LD dimensions and indicators resulted from water, tillage, soil, salinization, and overgrazing. Moderate (0.49) index of water degradation and the changing climatological index were light but the reduction of drainage density of...
Yuan Li1, Liyan Zhang2, Karhoe Loh3, Ji Feng1, Xinqing Zheng1, Puqing Song1 and Longshan Lin1,*
... the diverse habitats, unique haplotypes were ultimately formed under the differing pressures of natural selection. This study is expected to provide a basis for future research of the population genetics of P. minor.
...

Shaista Naz1*, Noor Paio Khan1, Naveed Afsar2 and Ashfaq Ahmad Shah3

...multistage sampling technique and data were collected from the selected respondents using a pre-tested semi-structured questionnaire through face to face interview. The collected data were subjected to analysis using descriptive statistics, frequencies, percentages, and rank orders. The results revealed that women were involved in buffalo, cattle, goats and sheep rearing with higher possession of small ruminants (goats and sheep), while men were found dominant...

Asif Nawaz* and Muhammad Zafarullah Khan 

...of Sekaran Sampling Technique and the respondents were distributed in four Agro-ecological zones of the province with the help of proportional allocation sampling method. Data from the respondents were collected through pretested and validated interview schedule. Data was analyzed using SPSS Ver.20. Mean, standard deviation, chi-square test and paired sample t-tests were calculated to achieve the study objectives. Results of the study revealed that Field Assis...

Amjad Ali1, Abbas Ullah Jan1, Lal Almas2, Noor Piao Khan1 and Khurran Nawaz Saddozai

...kelihood estimation techniques. Per acre total yield, land rent and per acre inputs cost (labor hired, seed, tractor hours, irrigation, Urea, DAP, FYM and pesticides) were considered determinants for cost function. Respondent age, farming experience, education, family size, off-farm income, farm to home distance, tenancy and extension contacts are determinants in allocative inefficiency model. Results of the study revealed that except farmyard manure and pesti...
Mehwish Aftab, Tariq Mahmud*, Muhammad Asim Raza Basra, Aqsa Gulzar, Sumaira Basharat and H.M. Junaid
...NMR, UV-Vis and ICP techniques were used the characterization and structure elucidation of the prepared Schiff base and its La (III) and Ce (III) complexes. The proposed geometries of the complexes were established on the basis of metal ligand ratio through ICP. Schiff base showed antioxidant activity against diphenylpicrylhydrazyl (DPPH). The results showed that metal complexes are good antioxidant agents as compared to Trolox (standard) and ligand was less a...
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
...lies of Lahore. The techniques used for the diagnosis of MCD were visual Acuity test by Snellen chart, phoropter, slit lamp biomicroscopy, topography, keratometer and pachymetry. In this study, 50 patients of MCD were identified among which 40 were males and 10 were females, including 9 cases with family history. Main complaint was drop in visual acuity and loss of vision in all patients (100%) due to damage and accumulation ofkeratan sulfate (KS) in macular l...
Muhammad Babar Khawar1, Muddasir Hassan Abbasi2*, Zillay Mariam1, Nadeem Sheikh1,2*
...ate is a brownish color liquid containing various toxic heavy metals and other chemicals of organic and inorganic nature which can house a variety of pathogens. As it drains it pollutes the groundwater table and if used for irrigation purpose represents a major health risk not only to humans but also to local flora and fauna. The purpose of current research work was to analyze the toxicity of leachate in Wistar rats. Wistar rats of about 250g were selected and...
Tahira Batool1*, Nabila Roohi1, Athar Mahmud
...nalysis of Variance technique (ANOVA) and means were compared by Duncan’s Multiple Range (DMR) test. The findings of this study revealed L3 lysine regimen to be the best for improving the slaughter characteristics including weight of head, shanks, lungs, liver, gizzard, heart, and carcass yield comprising percent weight of breast and thigh. 
...

Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1 

... and disk diffusion techniques while best effective seven were trialed in glass house and field experiments to determine their efficacy against Xanthomonas oryzae pv. oryzae. In vitro and in vivo studies under different conditions showed significant response of Mentha piperita, Azadirachta indica and Aloe vera either tested individually or in combination of two or more than two decoctions against the bacterium in question. When used individually, M. piperita d...

*Nageen Fatima1, Sadia Murawwat1 and Syeda Ameena Rizvi1

EVALUATION OF IMAGE COMPRESSION TECHNIQUES: AN IMPLEMENTATION OF TRUE COMPRESSION ON 2-D IMAGE
...ison of compression techniques is done using Compression Ratio (CR) and Bit-Per-Pixel (BPP) ratio as performance parameters. Image compression by Huffman encoding, Embedded Zero tree Wavelet (EZW) and Set Partitioning in Hierarchical Trees (SPINT) technique is performed. The result showed that lossy techniques are reliable to save hardware memory consumption but having low CR ratio. In los...

 *Irum Javed1, Muhammad Asgher2, Sadia Noreen1, Humara Naz Majeed1 and Tanzila Sahar1

SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE
...first step of study the liquid state fermentation (LSF) and solid state fermentation (SSF) by Aspergillus niger was compared using molasses based medium and the different carrier substrate. In a second set of study wheat bran was utilized for citric acid production via fermentation parameters was optimized. The results showed significant (P≤ 0.05) difference among LSF and SSF and between different substrates in LSF and SSF. Wheat bran showed significantly i...

 Sadia Murawwat1, *Nageen Fatima1, Qandeel Iqbal1, Syeda Ameena Rizvi1 and Mehak Fatima1

ELECTRICAL ENGINEERING BEYOND CLASSROOM: CHALLENGES AND ANTICIPATED RESOLUTIONS
...nds and pedagogical techniques to improve the status of techno-oriented Pakistan are also inspected.

...

 Mian Azhar Ahmad1†*, Nawab Mohammad Khan2, Asmatullah3

Anatomical variations of hepatobiliary triangle in patients operated laparoscopically for gallbladder diseases from Lahore and Sahiwal
...dy. Random sampling technique was employed to record the relevant information data. During the course of this study evaluation of 2500 patients, including 2350 women and 150 men, who underwent exploration of hepatobiliary triangle during laparoscopic cholecystectomy for different gallbladder diseases was performed. Total of 63.6% of patients expressed cystic duct, cystic lymph nodes and cystic artery variations. Among them 12% depicted cystic duct variations, ...

 Saiqa Andleeb* , Shaukat Ali

RNA interference; A tool for treatment of human diseases
... powerful and rapid technique to knockout the gene expression by introducing either short interfering RNA or double stranded RNA fragments into targeted host. These RNA fragments degraded specific homologous mRNA. RNAi technology used as an essential tool in several model organisms such as invertebrates, mouse and fly to explore the role of individual gene. In recent era, RNAi is directly used in therapy of various infectious and non-infectious diseases. Varie...

Durr-e- Samin Tahir*, Waqar Nasir, Sarah Bushra, Fatima Batool

Height trends in the population of Rabwah, district Chiniot, Pakistan and comparison with WHO standards
...> smoothing spline technique were applied to analyze the height trends of the collected data. The average height of
male adults is found to be 172cm (67.71" ± 3.98) and for females it is159 cm (62.55" ± 3.06).Comparison of male and
female height trend indicates that growth rate is almost the same up to 13 years, but it is higher for males after 13
years age. The female height trend revealed non-significant increase...

Dilara Abbas Bukhari1, Abdul Rehman2*

Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus
...s ostreatus. Exudate as liquid droplets on the mycelium was checked for antimicrobial activity against bacterial strain, Bacillus subtilus. Biochemical techniques, thin layer chromatography, high performance liquid chromatography and gas chromatography/mass spectrometry, were employed to study fungal exudate. A total of fifteen different metabolites were detected in the sample by GC/MS. Th...

Shaukat Ali1,*, Sundas Nasreen1, Sobia Safeer1, Saiqa Andleeb1, Mubashir Ejaz1, Saira Bano2, Hafiz Abdullah Shakir3

Medicinal plants as therapeutic agents for cancer treatment
...> medicinal industry, unique anticancer agents are being required from traditional medicines. According to recently
published data, this article reports a detailed review of ethnomedicinally important anticancerous medicinal plants. It
will provide a new way to explore the therapeutic value of plants and characterization of biologically active
compounds from them that may lead towards developing anticancer drugs and proper treatment of ca...

 Muhammad Khalil Ahmad Khan1*, Muhammad Zafar2, Munazza Perveen3, Munir Ahmad3, Asia Iqbal4, Asmatullah3

Efficacy of malathion and carbosulfan against crop invadedaphid types Aphis craccivora (Koch) and Aphis gossypii (glover) on bean and brinjal plants
...esidual
film technique with tested Aphid population. The most toxic insecticide was found
to be malathion with LC50 as 20.11 μg cm-2 for A. craccivora and 25.28 μg cm-2 for
A. gossypii while carbosulfan was found to be least toxic with LC50 as 312.80 μg
cm-2 for A. craccivora and 322.25 μg cm-2 for A. gossypii respectively. No
significant heterogeneity was to be found.

...

 Raazia Kiran1, Alya Riaz1, Muhammad Irfan1*, Hafiz Abdullah Shakir2

An overview of pre-treatment methods used for bioconversion of lignocellulosic biomasses into valuable products
...mical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bacteria, and quite a few combinations thereof to
breakdown the lignocellulose into its components. Pre-treatment process changes
cellulose morphological, chemical and physical features, making it prone to
enzymatic attack for saccharification. In this review, we have discussed different

Khush Bukhat Zahid1*, Ghaffar Ali2, Samina Sabir1 and Mohammad Fayaz2 

...production function technique is used to evaluate the relationship between climate and yield variability. The results show that higher amount of rainfall has a negative and significant impact on the production of all crops including wheat, rice and maize. It may be because unexpected rainfall in Pakistan during monsoon season severely damages crops. This result is consistent with our expectation that high level of rainfall would decrease agricultural productiv...

Akshay Sharma*, Mohit Mahajan, Madhumeet Singh and Pravesh Kumar 

...y and color Doppler technique, it is possible to determine the twins’ zygosity and viability. 

...

Abid Muhammad Shah1, Safdar Ali1*, Ijaz Ahmad2, Gulzada Wazir1, Obaidullah Shafique1, Muhammad Amir Hanif1, Bashir Ahmad Khan3 and Sumaira Zareen4 

...nalysis of variance technique and means were compared by using the least significant differences test (LSD) at 5% level of significance. Most of the studied parameters showed significant results (P≤0.05) for yield and weed indices. The results showed that highest weed control efficiency (41.86%) was recorded for hand weeding followed by Ally Max (30.8%) which were significantly different from each other; while, in case of sowing methods, weed control effici...

Neelum Andaleeb1*, Munir Khan1, Syed Attaullah Shah1 and Rahmat Ullah2 

...multistage sampling technique was used to collect a sample of 274 livestock farm households from six union councils of district Mardan. Data were collected through face to face interviews from female members of the selected livestock farm households in 2015-16. Data were analyzed by ordered logit model to determine factors affecting households welfare, measured as daily per capita income level. Variables, such as income generating individuals, milking animals,...
Faiz Muhammad1,2, Canfeng Dou1, Zhen-ming Lü1,*, Li Gong1, Xun Du1 and Muhammad Shafi3
...of the haplotypes were unique. This study solely provide species specific information on its genetic structure and population variability in Chinese waters.
...
Qingling Hu1,2,3,* and Jinian Feng3
...d. This new species is unique in this genus by the combinations of having 5 pairs of long setae on pronotum, the ultrashort chapped craspedum on posterior margin of abdominal tergites, and the shape and relative locations of setae on tergite IX.
...

Sahar Shibli1,2*, Farzana Siddique1, Saeeda Raza2, Zaheer Ahsan3 and Irum Raza4 

...t cultivars through gas liquid chromatography revealed six fatty acids namely palmitic acid, oleic acid, linoleic acid, arachidic acid, eicosenoic acid and behenic acid. The peanut cultivars Bard-479 and Local-334 were more suitable for oil extraction and peanut butter development because of their high oleic acid to linoleic acid ratio (2.3-2.4). Bard-92 was less preferable cultivar for product development owing to its high linoleic acid (42.56%) and low O/L r...

Mudassar Javed, Muhammad Zeeshan Majeed*, Muhammad Luqman and Muhammad Afzal 

...cards) and cultural techniques against okra lepidopterous borers. According to results, maximum shoot and fruit infestations (i.e. 19.86 and 15.63%, respectively) by okra borers were recorded in control (unsprayed) module, while minimum (i.e. 6.76 and 2.89%, respectively) were found in IPM module. Mean shoot and fruit infestations in farmers’ routine module were 13.91 and 10.83%, respectively during 2016. Similarly, in 2017, mean shoot and fruit infestat...

Zakiullah1, Muhammad Farid Khan1*, Muhammad Mohibullah3, Muhammad Iqbal2, Irfanullah3, Faheemullah3, Madiha Urooj1 and Uzma Arif1 

Raza Ullah1* and Jamal Shah

...multistage sampling technique and Analysis of Variance was used to compare the average wheat yield of farmers using different combinations of the two risk coping tools namely, off-farm diversification and precautionary savings. The results suggested that adoption of risk coping tools, particularly the simultaneous adoption of multiple tools at the same time, significantly reduced wheat yield. The research suggested that future studies should identify ways to m...

Shumaila Nazeer1, Iqbal Javed1*, Allah Bakhsh2, Mudassar Yasin2, Haroon Javaid3 and Kaniz Fatima1 

...convenient sampling technique. Along with export margin analysis of both markets, an effort is made to quantify impact of the major determinants on export of mandarin (Kinnow) to UAE and USA. According to data properties and suitability, multiple regression analysis technique is used for UAE and binary logistic regression technique was used for USA market. According to the finding, gross e...

Shamsher Ali1*, Muhammad Jamal Khan2, Zahir Shah2, Naveedullah3 and Abdul Jalal1 

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Nosheen Fatima1, Raheel Babar3 and Ikramul Haq4  

...not know the modern techniques to uphold crop and species diversity. Biodiversity concept of farmers is at very basic level and they know only limited conventional ways to maintain it. The statistical analysis showed weak significant association between factors responsible for biodiversity loss and two types of biodiversity since χ2 (3) = 11.338, p = 0.010 and concluded that all factors are not equally responsible for loss in both types of biodiv...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...sitive and reliable technique for the determination of circulating miRNA expression. Despite recent advancements, there is not a single consensus on the use of reference gene for quantification of circulating miRNAs through qPCR analyses in ALL. In the current study, we identified the reference gene that is the most suitable for qPCR normalization in patient and control plasma samples of ALL. Three highly reported reference genes namely RNU6-2, mir-16-5p and <...

Farhan, Shahid Ali* and Syed Attaullah Shah 

...ied random sampling technique. Primary data was collected through well designed interview schedule. Normalized restricted trans log profit function was first approximated for further estimation of output supply and input demand elasticities. Results indicated that response of output supply to the price of wheat and land was positive and elastic. Output supply in response to wage rate was negative and inelastic. Output elasticity in response to fertilizer price...

Noor ul Ain*, Malik Muhammad Shafi and Haidar Ali* 

...oportion allocation technique a total number of 120 households were selected from two villages namely Landi Arbab and Deh Bahadar. Data was analyzed through Double log model for estimation of demand for the selected vegetables. It was found that household head in Landi Arbab includes 87.10% male and 12.90% female whereas in Deh Bahadar, 94.82% household heads were male and 5.18% of the household head were female. Descriptive statistics showed that the average ...

Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3* 

...timized inoculation techniques are required. The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly influenced by pathogen inoculation method. The infection caused by S. scitamineum and subse...

Muhammad Ali1,2*, Norsida Man2 and Farrah Melissa Muharam2 

...ge cluster sampling technique. In order to measure motivation of respondents, likert scale items comprising 1 as strongly disagree to 5 as strongly agree were used. The research findings reveal that farmers were motivated to manage their agricultural risks and overall their motivation level was high. Nevertheless, still efforts are required by all stakeholders to keep the motivation level high of farming community at local, regional and international level. Ag...

Rehmat Ullah* and Khalid Nawab 

...ulti-stage sampling technique. Furthermore, four union council viz. Band Kurai, Baidara, Khanmai and Baffa from the sampled districts were selected. A total of 384 respondents were selected through unknown population sampling formulae i.e. 96 from each Union Council. Data were collected using well-structured interview schedule through personal interview method whereas binary logistic regression model was utilized (binary responses (1 if exists 0 otherwise) bot...

Shakeel Ahmad Anjum1, Muhammad Mohsin Raza2, Sami Ullah1, 3*, Malik Muhammad Yousaf2, Ahmad Mujtaba1, Mumtaz Hussain2, Muhammad Jahangir Shah2, Bashir Ahmad2 and Ijaz Ahmad4 

... is mainly based on techniques used for good seed bed preparation. The objective of this study was to check the influence of various tillage practices on yield of the autumn planted maize crop. The experiment was conducted in summer, 2014 at Agronomic Research Area, University of Agriculture Faisalabad, Pakistan in a randomized complete block design (RCBD) with four replications. Net plot size was maintained 7.0 m × 3.6 m. Different tillage practices, i....

Rafiullah1*, Abdur Rahman2, Khalid Khan1, Anwar Ali1, Arifullah Khan2, Abdul Sajid3 and Naimatullah Khan3 

...ifferent diagnostic techniques, including Microscopy, ELISA and Real-time PCR for diagnosis of Theileria parva at Veterinary Research Institute Peshawar. The overall prevalence for Theileriosis was 27.75% through Real-time PCR, followed by Indirect ELISA (24.62%) and Microscopy (11.37%). Transect wise high prevalence for Theileria parva was observed in Lakki Marwat with 33.5% for Real-time PCR, significantly different at P<0.05 from Peshawar. Cattle showed ...
Fariha Latif* and Muhammad Javed
... use of biochemical techniques along with antioxidant parameters has gained considerable attention due to their use as biomarkers to assess contaminants impact on fish at earlier level of exposure. Fish from different species has diverse ecological needs and respond differently to the environmental stress conditions. Thus, the sensitivity, bioaccumulation and antioxidant capacities of three major carps Catla catla, Labeo rohita and Cirrhina mr...
Muhammad Usman Ahmadand Ikram-ul-Haq*
...y employing Hungate Technique where its antioxidant potential was compared with common reducing agent Cysteine-HCl (Cys-HCl). Four anaerobic bacterial strains (Anaerobaculum hydrogeniformans OS1, Akkermansia muciniphila, Bilophila wadsworthia, and Roseburia intestinalis) were selected as experimental models for this purpose. Significant improvement in cell density of Anaerobaculum hydrogeniformans culture with maximum OD...
Shahid Yousaf1*, Salman Khurshid1, Saqib Arif1, Hafiza Mehwish Iqbal1, Qurrat ul Ain Akbar1, Najmus Sahar1 and Ghulam Rasul2
...ing and trade. Many techniques have been introduced to evaluate wheat quality. Near Infrared (NIR) is one of the suitable techniques that have an advantage of rapid response over other techniques. But investigations are needed to check its reliability on determining important quality parameters. The study therefore has been designed to evaluate the efficiency of NIR in comparison with stan...
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
...ision Making (MCDM) techniques combined with the data fusion technique support the proposed hybrid approach’s results. In this context, the proposed hybrid approach was applied to evaluate the monthly production performance of a commercial enterprise with Lohman Brown genus 8000 chickens. According to the findings of the analysis, it was seen that January, March, October, November and December are the months when produ...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
... spectrophotometric technique.
...
Catherine J. Allen
...lectuals united in a critique of neoliberal development paradigms. Ecuador and Bolivia have enshrined sumak kawsay and suma qamaña in their national constitutions. How does this political discourse correspond to indigenous notions of well-being experienced in the socio-culturally diverse communities of the Andean region? I begin by focusing on inhabitants of Sonqo, a rural Quechua-speaking community in the highlands of southern P...

Dilshad Ahmad1* and Muhammad Afzal2

...ges random sampling technique used for data collection of 400 Bt and Non-Bt cotton farmers for the year of 2012-13. The results of the study have indicated Bt cotton farmers of both districts with higher technical efficiency score as more efficient than Non-Bt cotton farmer. Empirical findings specified a cropped area, seed, labor, fertilizer and irrigation were the significant variable of increasing cotton production of Bt farmers while pesticides, irrigation...

Dilshad Ahmad1* and Muhammad Afzal2 

...ple Random Sampling technique was used in the study for selecting the respondents in both districts of cotton area. Pre-tested questionnaire was developed for the collection of data 320 cotton farmers’ respondents for the year of 2015-16 including 160-credit user and 160 non-credit users. The study employed stochastic production frontiers function for the empirical estimates of credit on cotton production. Empirical estimates of study have denoted, signi...

 Syed Turab Raza1,*, Zhu Bo1,*, Zulfiqar Ali2 and Tang Jia Liang3

...tida) is useful technique to recover nutrients of plants such as NPK (nitrogen, phosphorus, calcium). A vermicomposting system using the earthworm species (Eisenia fetida) and treating it with cattle dung, pig manure and biochar with crop (wheat straw and rapeseed) waste was established in upland areas of China. It was monitored for two months. Four treatments (T1 to T4) were prepared using crop residues i.e. wheat straw, rapeseed and cow man...

Muhamamd Naeem1 and Murad Khan2* 

...stic as well as OLS technique was used. The main cost of winter season consists of feed cost, lighting cost, the price of day old chicks, breeding cost, medication and vaccination cost. These costs were high in the winter season than the summer season. The mortality rates were also high in the winter season. The lighting cost was comparatively less in summer season. Broiler and breeding cost was also low. The consumption of breeding was less and the price of b...

Salman Anwar1, 2*, Farhan Anwar Khan3 and Atta-ur-Rahman4 

...bsistence and crude techniques were applied due to non-availability of modern machines and techniques. This was because of the remoteness and communication gap with the rest of the country. Therefore, this study is an effort to compare and analyze the impacts of KKH on the land use and Agricultural development of Gilgit-Baltistan before and after the construction of KKH. Results show a significant change in land use and deve...

Arjumand Nizami1, Jawad Ali1 and Muhammad Zulfiqar2* 

...ffective management techniques for on-farm water. 

...
Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
...n leaf dip bioassay technique was used. However, among tested botanicals, Azadirachta indica oil showed better results with percent CLM mortality of 35.6%, through topical bioassay and 31.8% through leaf dip bioassay. In the case of A. indica, the LC50 value was also observed lower (1.88±0.37, 1.73±0.29) in LDB and TB, respectively, as compared to other botanicals. So, our study suggested that abamectin gave better...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

...sing real time-qPCR technique in which miR-specific DNA primers were used to increase sensitivity of reaction. Expression of miRNA panel was normalized with mir-16 and expression fold change was calculated by 2-ΔΔCt method. Further analysis was done by SPSS, medcalc and Graphpad prism software. Area under curve analysis was used to get diagnosis outcome of selected miRNA panel. Upregulation of miRNA panel (mir-376c, mir-155, mir-17a and ...

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

... by bacteriological techniques on positive milk samples for confirmatory isolation of Staph. aureus as a bovine mastitis causing agent. These Staph. aureus isolates were further tested for antibiotic susceptibility. Epidemiological data was analyzed by chi-square methods at 95% confidence interval. The overall prevalence of subclinical mastitis as a result of surf field mastitis test was 42.2%. The prevalence of disease was higher in cows (54.5%)...

Bata Shalangwa Ishaku1, Batim Turdam1, Maimadu Abdullahi1, Waziri Ibrahim Anjili2 and Mayowa Olabode2 

...n and sedimentation techniques showed that 276 (92.0%) were positive for gastrointestinal nematodes. A greater proportion (70.7%) of the nematodes were Strongyles followed by mixed infection of Strongyles and Parasacaris equorum (15.2%) and Strongyloides westeri (14 1%). Chi-square test and odds ratio were used to analyzed data obtained. The prevalence of the gastrointestinal nematodes was not found to be associated with factors such as sex, age and location w...
Muhammad Shahid Nadeem*, Maryam A. Al-Ghamdi and Jalaluddin Azam Khan
...
L-asparaginase is ubiquitously found in microbes, animals and plants. The asparaginase of bacterial origin has been extensively applied in the treatment of childhood lymphomas and leukaemia. However, the glutaminase activity of enzyme can cause serious side-effects triggering a search for highly specific and more stable enzyme. In the present study the gene consisting of 972bp coding for 323 amino acids was PCR amplified from Geobacillus thermodenitrif...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...erential attachment technique. Cell morphology were observed using an inverted microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDSCs were induced into Adipocytes, osteoblasts, chondrocytes and neuron-like cells by the optimized inducing medium. ...

Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1* 

...I-based vaccines apply unique selective pressures on NDV glycoproteins and their role in changing the NDV evolution in Egypt. 

...

Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1 

...e by dilution plate technique. Among isolated isolates, 6 representative colonies were purified, multiplied and characterized using light microscopy and other biochemical tests. The bacteria were tentatively identified as Pseudomonas, Streptomyces, Bacillus and Micrococus. These bacteria were checked for their antagonistic activity against Fusarium oxysporum f.sp. alium cepa. They all were found to inhibit fungus showing inhibition zone 4.09 -74.97%. Hence, on...

Arshad Farooq* and Muhammad Zafarullah Khan 

...on and cane cutting techniques. Medium knowledge gap (21 to 50%) was observed in length of cane setts, row to row spacing in farmers of D.I. Khan district while in farmers of Mardan district medium knowledge gap was observed in land preparation, improved cane varieties, row to row spacing and stoppage of irrigation before harvesting. High knowledge gap (above 50%) was revealed in cane setts treatment, dept...

Hafiz Umar Farid1, Muhammad Zubair2, Zahid Mahmood Khan1, Aamir Shakoor1*, Behzad Mustafa1, Aftab Ahmad Khan3, Muhamad Naveed Anjum4, Ijaz Ahmad5 and Muhammad Mubeen

... these water saving techniques for the welfare of mankind. 

...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...y and real-time PCR techniques. MTT spectrophotometric tests showed no significant effects on the survival rate of T-98G cells in 24 and 48 h. Therefore, T-98G cells were not analysed by flow cytometry and RT-PCR. When LNCaP cells were exposed to 1, 5, 10, 25, 50 and 100 μM doses of TA for 24 h, the rates of living cells were determined as 91%, 83%, 82%, 76%, 61% and 49%, respectively. When the same doses were applied for 48 h, the living cell rates were 90...

Tariq Shah1*, Umar Hayat1, Muhammad Suleman Bacha2 and Muhammad3 

...riptive statistical techniques along with independent sample t-test and dummy variable regression were used for analysis. It is observed during the study that livestock extension department and private livestock doctors are the key sources where farmers get advice regarding livestock production. Majority of the respondents are quite satisfied regarding the effectiveness of these advices. Results further indicate that farmers visit or contact livestock expert m...

Shahid Ali1*, Asim Khan1, Aftab Khan1 and Bakhtawar Riaz

...allocation sampling techniques. Data was collected by using a well-structured interview schedule. Cobb-Douglas production function was employed to assess technical efficiency. Maximum likelihood estimation techniques were employed for estimation. Results showed that all explanatory variables except pesticides have significant effect on tomato production. The results showed that a 1% increase in seed rate, labor, tractor hour...

Ayat Ullah* and Ayesha Khan 

...age random sampling technique data were collected from 395 respondents according to Yamane’s formula. The study uses the Kruskal–Wallis H test for checking differences among farmers knowledge with differences in their contact with extension service providers. The results revealed significant differences in farmers’ knowledge of chemical, biological and cultural control methods of pest with differences in the frequency of extension-farmers&rsq...

Zahin Anjum1*, Farhat Shehzad1, Amina Rahat1, Hamid Ullah Shah2 and Saleem Khan3 

... different grilling techniques were applied to see their effectiveness in lowering the levels of these toxicants. Polyaromatic Hydrocarbons and heavy metals refer to a large group of chemicals that may be present in the environment as pollutants and can enter the food chain and might be carcinogenic to humans. The lead content was found high in fish with the mean value of 3.18mg/kg and the lowest values were observed in chicken with mean value of 1.38mg/kg. Th...
Iram Gull*, Muhammad Shahbaz Aslam, Imran Tipu, Roohi Mushtaq and Muhammad Amin Athar
...the results of this technique are not always reliable due to inefficient overlapping of the sequences especially those derived from authenticated native sequences. Certain forbidden sequences do appear at junction of spliced genes whenever efficiency of gene fusion is improved by inserting GC rich sequences. In this study, reliable scar-less gene fusion with the insertion of sequence of choice at junction sites has been described by optimization of various par...
Iqra Jaffar, Zainab Sehzadi, Muhammad Adeel, Khalid P. Lone and Mehwish Faheem*
...different sources, are ubiquitous in the aquatic environment. In the present study, freshwater fish (Labeo rohita) juveniles were exposed to low concentrations (10μg/l and 100μg/l) of di-n-butyl phthalate (DBP) for 7 days to evaluate oxidative stress in liver and gills by analyzing activity of glutathione-S-transferase (GST), catalase (CAT), glutathione (GSH) and by measuring lipid peroxidation (LPO). Catalase activity increased significantly in l...

Essossinam Ali* 

...ulti-stage sampling technique was used to collect data from 704 farm households in three regions (Central, Kara and Savannah) in subsistence agriculture in Northern Togo. Tobit and linear regression models were used to analyze the effect of farmers’ risk aversion on fertilizer and DTS uses, and labour allocation, respectively. The results indicate that on average 86.22% of farmers have risk aversion behaviors. Widening the land devoted to maize cultivati...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
...tube method compared to liquid interface coverslip assay (35%) and congo red assay. D. vulgaris was treated with various concentrations (5, 40, 80 and 100µM) of salicylidene acylhydrazide and 5-15 µM of proteases (proteinase K, trypsin, and chymotrypsin). Concentration dependent decrease was observed after 72 hours in biofilm formation of D. vulgaris on treatment with salicylidene acylhydrazide. Whereas 15 µM concentration of pr...

Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3* 

...scripts in flower and siliques while no expression in the leaf tissues was observed. A strong expression for FUL gene was detectable in mature silique and silique from upper portion of plant as compared to other tissues of “Pakola” and “Punjab Sarsoon 3”. Our results flaunt basic gene expression information about shattering genes for developing genome edited plants ...
Ghalia Nawal1, Muhammad Irfan2*, Saira Ashfaq3, Hafiz Abdullah Shakir1    
...lan involves Sub-merged liquid fermentation in fed batch manner using agricultural wastes. Bacterial lipases are preferred due to their extra cellularity, easy step down approach and practicability of increased productions via technological modifications. They have wide- ranging applications in diverse sectors of the food, medical, cosmetic, detergent sectors and in paper industry which establishes their irrefutable noteworthiness. 
...

Kamran Aziz1*, Aamir Saleem1 and Arshad Mahmood Malik2 

...t, 2017. Litter bag technique was used to estimate the decomposition rate, elemental composition was carried through chemical analysis in the laboratory and litter fall production was estimated by trapper technique. Under Cedrus deodara and Pinus wallichiana canopy, the leaf litter was collected and brought back to laboratory for the further chemical analysis. The Total annual litter fall production of Cedrus deodara was 4.2...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...phate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT remain lowest in exposed fish as 134.30±0.71 Unit mg-1in relation to control (148.02±0.41 Umg-1). The fold purificati...
Proceedings of Pakistan Congress of Zoology

Umar Hayat1*, Tariq Shah1, Muhammad Suleman Bacha2 and Muhammad

...dinary least square technique is used to estimate the model. The result shows that Institutional credit is playing a significant role in achieving agricultural growth in the country. The other variables of the model like agro-based industries, livestock, mechanised inputs and agricultural exports have shown a positive impact on agricultural growth and ultimately on the overall GDP growth of the economy. The government needs to allocate more funds for instituti...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...irectional freezing technique (UFT).
...

Muhammad Usman Ghazanfar, Muhammad Imran Hamid, Mubashar Raza, Waqas Raza*, Misbah Iqbal Qamar 

...widely distributed and ubiquitous in almost all type of soils and their species promote growth of the plants and commercially used as bio-fungicide against soil borne plant pathogens. The present study was conducted with the aim to determine the efficacy of Trichoderma species using dual culture and pot assays against Phytophthora infestans (late blight) and Fusarium oxysporum (wilt) of tomato on different compost including carbon rich compost, nitrogen rich c...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5 
...en was split into four aliquots in which tris-citric acid extender (TCA) was supplemented with various concentrations of L-cysteine 0 mM, 0.5 mM, 1.0 mM and 1.5 mM. Cooling and equilibration of the diluted semen was done at 4°C for 4 h subsequently filled in straws at the same temperature and were cooled in liquid nitrogen vapors for 10 min. followed by storage at -196°C for assessment. Progressive motility an...

Haidar Ali1, Malik Muhammad Shafi1*, Himayatullah Khan1 and Hamra Haidar

...ns. Random sampling technique was used for selecting a sample size of 195 small farmers. The present study is focused on two selected districts of Peshawar Valley namely Mardan and Peshawar. Analysis showed that there was a significant difference in the off-farm employment among various sizes of farms. Sampled farm households who were operating farm size up to 1 acre (74.88 hours/week) were performing more off-farm employment followed by farm size more than on...

Zara Ahmad1*, Fazli Rabbi2, Muhammad Zamin3, Bushra Kiran2, Tariq Shah2 and Shahzad Kouser4 

... Least Square (OLS) technique. The results show that there is a significant difference between the on-season and off-season application of pesticides to bitter guard crop. The results clearly reveal that bitter guards’ yield is significantly higher in the off-season than on-season. It was also found that off-season bitter guard fetches 66% higher price in the market than on-season bitter guard. The analysis also revealed that the cost of pesticides is 43...

 Aftab Khan1, Shahid Ali1, Murtaza1, Sufyan Ullah Khan2 and Syed Attaullah Shah1

...tage stage sampling technique for choosing the sample size. The data was collected from 200 respondents using a well–designed interview schedule. Cobb-Douglas type production function was modelled to estimated allocative efficiencies of individual inputs used in maize production. The estimated allocative efficiencies of Urea and DAP were 3.24 and 2.04, respectively. This implies that these inputs were underutilized that might be due to poor farming skill...

Sajjad Ali1, Atta-ur-Rahman1 and Sher Ali2* 

... 1985-2015. Spatial techniques like Geographic information system and remote sensing are used to detect land use changes. This study is carried out at three levels; initially the whole tehsil is studied for land use change analysis by comparing 1985 and 2015 Landsat images. Secondly, four sample union councils were targeted for medium level analysis. Thirdly, seven sample villages are targeted for micro level. The Landsat images of 1985 and 2015 are obtained f...

Muhammad Sharif1*, Sadullah1, Ghulam Rasool2, Muhammad Nasir Khan1, Zubair Rehman1, Ahmed Khan2, Ijaz Ahmad3 and Khalid Hussain4 

...resource conserving techniques. Therefore, an experiment was conducted under the treatments, i.e. conventional tillage (CT), Zero tillage (ZT) and Chisel plough (CP) during the year 2016 at the field research area of Balochistan Agriculture College, Quetta. The study was designed to compare conventional versus conservation tillage practices and their effects on soil physico-chemical properties such as total organic carbon (TOC), infiltration rates, soil water ...
Abdul Waheed1,2, Sidrah Saleem1, Naveed Shahzad3, Junaid Akhtar4, Muhammad Saeed5, Iqra Jameel1, Farhan Rasheed6 and Shah Jahan7,*
...s was tested by PCR technique. Every isolate was ESBL positive and resistant against third generation cephalosporin and Aztreonam. Notably, 15% of ESBL producing strains harbored blaSHV gene while 43% were positive for blaOXA gene. In a nutshell, diverse variety of ESBL-positive GNRs is distributed, the findings of the present study should be considered for planning strategies to treat and prevent the further spread of ESBL-...
Fiza Asif1,*, Muhammad Siddique Awan1, Nasra Ashraf1, Nuzhat Shafi1, Abdul Rauf1, Khizra Bano1, Muhammad Razzaq2 and Naeem Iftikhar Dar2
...g microhistological technique. Sixty fecal samples were collected from the study area during summer 2015 and winter 2015-16, i.e. thirty samples during each season. These samples were analyzed for the determination of food composition using microhistological technique. A total of 23 plant species were observed during summer and 15 plant species during winter season. During both the seasons Indian or Himalayan Chestnut...

Muhammad Zeeshan Manzoor1*, Ghulam Sarwar1, Mukkram Ali Tahir1, Noor-Us-Sabah1, Ayesha Zafar1 and Sher Muhammad2 

...t leaching fraction technique proved useful to mitigate the ill effects of brackish water. All the collected data were subjected to statistical analysis.  

...

Muhammad Ali1*, Norsida Man2 and Farrah Melissa Muharam

...ge cluster sampling technique. The subjective norms were measured through 5 points Likert scale (ranging from 1 as strongly disagree to 5 as strongly agree) based statements. The result exhibited that farmers had realized the significance of ICTs which helped them in staying connected with fellow farmers and extension service providers. Furthermore, majority of the farmers had a high level of subjective norms influence to use ICTs for agricultural risk managem...
Huaisheng Zhang1, Pengfei Li2, Huajun Wen2, Guangming Tian1, Hui Chen1, Lu Zhang1 and Jianqiang Zhu1,*
...vidianus). It is a unique species in China, which has been extinct in China for nearly 200 years. In 1980, the Milu was reintroduced into China and has now become a first-class protected animal in the country. Moreover, research on the Milu has begun. After 30 years of breeding and development, the Milu population has formed stable captive or wild populations in Beijing, Dafeng, Shishou and Dongting Lake. It has become a typical example of the successful r...
Muhammad Khalid Rafique1, Rashid Mahmood1,*, Ziyad Abdul Qadir1,Imran Bodlah2 andFarid AsifShaheen2
...our larval grafting techniques, addition of royal jelly (A), dry grafting (B), grafting with addition of one drop of distilled water (C) Royal jelly plus distilled water (D) on queen bee rearing were investigated under field conditions. A considerable variation in queen rearing success was observed as calculated by percentage of secluded queen cells vs. grafted larvae over the study interlude. The highest larval acceptance 80.85 ± 0.64 and 81.25 ±...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...ed by ultra-performance liquid chromatography (UPLC) and Bruker micOTOF OllQ TOF mass spectrometry, respectively. The full amino-acid sequence of the monomericpeptide was analyzed by sequential Edman degradation using a protein/peptide sequencer. The antibacterial activity of the protein monomer in antlerplate was studied by disc diffusion method, and the antibacterial ring diameter of each antibacterial agent was measured to determine its antibacterial abilit...
Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
...ing technology and its unique advantages will make molecular markers as novel and highly-efficient approaches for assessment of stock enhancement.
...
Muhammad Waseem1, Ahmed Raza1, Hamera Aisha1, Muhammad Naeem Awan1, Tariq Ahmad2, Rabia Nazir2 and Tariq Mahmood2,*
...tlight and tracking techniques (42% respondents). Summer is the best season for poaching the pangolins (44% respondents). Forest is best preferred habitat of pangolin (54% respondents) and pangolins selling and buying points are located outside the villages (63% respondents). Nomads, semi-settled people at different locations in the Potohar Plateau are actively involved in pangolin trade and poaching. Approximately 275 pangolins were poached at 46 different lo...
Khalid Khan1*, Hazrat Yousaf1, Noor Mohammad1, Munir Khan1 and Do Thi Thao2
...tributed Lag (ARDL) technique. Empirical results indicate that money supply has a significant and positive impact on food prices, as food prices are unswervingly linked to food security, real disposable income of households’and food production. The results of the study also revealed that the implication of monetary policy will be a significant instrument to influence poverty, food security, and consumption patterns in China. 
...
Ambreen Leghari1,2, Asghar Ali Kamboh2,*, Shakeel Ahmed Lakho1, Faiz Muhammad Khand1, Kanwar Kumar Malhi2,3, Iqra Bano Chandio4, Seema Baloch5 and Jan Mohammad Shah4
...revealed by culture technique showed a significantly (P< 0.05) higher percentage in >4 parity than 1 parity or 2-4 parity, having 4-8 liters/d milk production than those having 2-4 or >8 liters/d, and in late stage of lactation than early or mid-stage. In brief, BTB is prevailing in both Hyderabad and Tando Allahyar districts; however it is relatively higher in Hyderabad district than Tando Allahyar district. Infected animals shed more M. bovis...
Muhammad Mansoor1, Khalid Usman2*, Shahid Hameed Khan Khalil3, Abdul Mateen Khattak4, Muhammad Ehsan Elahi1, Muhammad Waqas Imam Malik2 and Amir Hamza2
...e preferred due to its unique taste and the highest fruit yield and the biggest fruit size; needs curing under controlled conditions. 
...
Shad Mahfuz1,2, Shuyuan Wang1, Mo Chen1, Fei Zao1, Dong Zhen1
Zhongjun Liu4 and Hui Song1,3*
...e used at 4%level as a unique feed supplement to improve egg quality, and health status of laying hens.
...
Faheem Aslam1*, Aneel Salman1 and Inayatullah Jan2
...itional econometric techniques face significant challenges in out of sample predictability tests due to model uncertainty and parameter instability. Recent studies introduce several machine learning algorithms to improve time series prediction accuracy. The purpose of this study is to develop a precise wheat production model using artificial neural networks (ANN). A total of 71 years’ wheat production data from 1948 to 2018 is divided into training ...
Taimoor Khalid*, Asad Ullah and Mussawar Shah
...ple random sampling technique. Perception of the respondents regarding illegality in forest management (independent variable) and forest governance (dependent variable) were measured on three level likert scale. Chi-square test was applied to test the association between the study variables. The results show that poor forest governance had highly significant association with people cut trees by legal permits (P=0.000), due to illegal forest use the environment...
Shagufta Naz1, Ayesha Javed1, Ayesha Saleem1, Khadija Murtaza1, Rukhama Haq1, Akbar Hayat2 and Neelma Munir1,*
...f the sterilization technique involves total demolition of all microorganisms and their spores in foods. In recent study garlic samples were treated with three different doses of gamma radiation such as 0.5, 1 and 2 kGy. Effects of gamma radiation were analyzed for different aspects like microflora, moisture, fat, ash, fiber, phenolic content, sprouting inhibition, physiological weight loss, pyruvate analysis and sugar contents. Gamma radiation affected these ...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
...zation. Collagen has a unique triple-helix structure composed of three polypeptides, and hydroxylation of proline residues is vital for the stability of this triple-helix. Prolyl-4-hydroxylase (P4H) is an enzyme with an α2β2 tetramer arrangement that functions to post-translationally hydroxylate proline residues in the collagen chain. The α subunit (P4Hα) of the P4H α2β2 tetramer contains the catalytic site, whereas the β...

Muhammad Zubair1, Sagheer Ahmad2*, Awais Rasool1, Muhammad Asad Farooq1 and Ibni Amin Khalil3
 

...onment (GGE)-Biplot Technique to identify best potential varieties of sugarcane for a region. Nineteen varieties of sugarcane were assessed in seven environmentally different locations of Pakistan for their yield response. Experiment at each location was carried out in randomized complete block design (RCBD) with three replications. The environment (E) explained 16.1% of the total variation whereas G and G × E interaction captured 48.2 and 34.5 %, respec...
Zelle Huma1*, Ahmad-Ur-Rahman Saljoqi1 and Farman Ali2
...atodes, Insect bait technique was used for Entomopathogenic nematodes collection. For this experiment, Galleria mellonella larvae were reared in the laboratory. When  samples soil were collected from different regions of Khyber Pakhtunkhwa for nematodes collection G. mellonella larvae were left in sampling jar and were collected back after 2-3 days’ post application. Dead larvae were then put on the white trap and EPNs has then collect...
QihangLiu1,2, YueliJiang2, Tong Li2, Jin Miao2, Zhongjun Gong2, Yun Duan2, and YuqingWu2*
...ocused on different techniques to capture large numbers of locusts to avoid such swarms. The exploitation of locusts phototactic response has been one such technique. The current study investigated the impact of different light wavelengths and intensities on the visual and thoracic temperature responses of locusts. Our results showed that ultraviolet (UV), violet, and orange light stimulated the visual system of the locust a...
Ahmad Ali1,2,* and Aleem Ahmed Khan1
... different trapping techniques and lure used in capturing of falcons were documented in various districts of Punjab, Pakistan where falcons are commonly captured and traded. Total eight falcon capturing techniques i.e. bal-chatri, dho-gaza, piddam, jacket, socks-ball, nest-net, adda and laggar falcon (Falco jugger)/white eyed buzzard (Butastur teesa) as bait technique were re...

Wajid Shah Yousafzai1, Irfan Ullah1*, Amjad Ali1, Mahmood Iqbal2 and Salman Khan1 

...multistage sampling technique cross sectional data was collected from 105 sample respondents in three villages (Aboha, Manyar and Parrai) of Tehsil Barikot, district Swat. Simple Linear Regression Model was employed to individual input contribution in total output. The concept of Marginal Value Products and Marginal Factor Cost was used to calculate Allocative Efficiency. Ordinary Least Square technique was used to drive lin...

Shahid Ali1*, Farhan1, Murtaza1, Neelum Andaleeb2 and Amjad Ali1 

...age random sampling technique was used to collect data from 160 respondents. Stochastic frontier production (SFP) function was used to analyze the technical efficiency of wheat growers. The Maximum Likelihood estimates of the model-1 showed that seed quantity, urea, chemicals (Pesticides and weedicides), labor and tractor hours were statistically significant with the coefficients of -0.027, 0.090, 0.060, 0.223 and 0.056, respectively. The estimated results of ...
Shaukat Ali1,*, Sadia Batool2, Fatima Javed Butt2, Sundas Nasreen1, Hafiz Muhammad Tahir1
...hers are looking for a unique treatment strategy for diagnosis and treatment of cancer; it is a second chief cause of deaths in the world. Advances in chemotherapeutic agents, various radiological techniques and bio-nanoparticles treatments (i.e., green synthesized metallic nanoparticles) attracted many researchers to conclude a novel cancer treatment strategy. Unluckily every strategy had some working limitations as a resul...

Ankur Abrol, Madhumeet Singh, Akshay Sharma* and Pravesh Kumar 

Hafiz Muhammad Tahir*, Palwasha Jabeen, Chand Raza, Shaukat Ali
...rn biotechnological techniques including of making transgenic organisms.
...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...hrough high performance liquid chromatography (HPLC), somatic cell count (SCC) and total viable count (TVC). Blood samples were analyzed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels. Aflatoxin M1 (AFM1) was detected in all milk samples of the Group B, C, and D in concentration higher than 0.05 ppb. The AFB1 was excreted in milk as metabolite AFM1 @ 1.35-1.59%. Udder health and milk quality deteriora...
Shahid Sherzada1,2,4,*, Muhammad Naeem Khan4, Masroor Ellahi Babar2, Muhammad Idrees3, Abdul Wajid2, Muhammad Nauman Sharif3, Muhammad Shahzad Iqbal3, Iram Amin3 and Muhammad Shahid3
...efficient molecular technique for the exact identification of typical fish species.
...

Mahnoor Pervez and Farkhanda Manzoor* 

...hrough high performance liquid chromatography analysis. Pollen and nectar were collected from 2016 to 2017 from different agricultural areas of Pakistan during a field survey. Pollen was collected by installing traps and nectar was collected through capillary method allied with centrifugation. The HPLC technique was used for fast and simple detection of pesticide residues of commonly used pesticides (imidacloprid, deltamethr...

Sanaullah1, Fazli Rabbi2*, Shahid Ali2, Zeeshan Khan3 and Muhammad Zamin4 

... poisson regression techniques were used for data analysis. The results reveal that 98 percent of the sampled tourists were willing to pay for the development and conservation in the area. Moreover, 37 percent of the sampled tourists were willing to pay Rs.100 (0.94 US$) per visit as an entry fee. The analysis further showed that an estimated amount of 421 million Rupees (3.97 million US$) could be generated as consumer surplus. The poisson regression estimate...

Urooba Pervaiz1*, Madeeha Iqbal1 and Dawood Jan2 

...Multistage Sampling technique was used to select the required sample, 80 farmers were randomly selected from five villages and data were collected through interview schedule. It was found that majority farmers were literate up to middle; young farmers showed more positive attitude towards extension recommendations, 93% farmers knew extension workers both by name and face and also knew extension recommendations, but they faced problems like small land holding, ...
Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*
...i> cyst wall, a shotgun-liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was applied and all identified proteins were categorised using a bioinformatics approach. The results showed that 3,472 proteins were identified with a theoretical molecular weight of 11.53–610.90 kDa and an isoelectric point of 4.13–11.77. A total of 63.07% or 50.09% proteins were annotated by Kyoto Encyclopaedia of Genes...

Ghulam Murtaza1*, Ghulam Sarwar1, Noor-Us-Sabah1, Mukkram Ali Tahir1, Fakhar Mujeeb2, Sher Muhammad3, Muhammad Zeeshan Manzoor1 and Ayesha Zafar1  

...tatistix 8.1, ANOVA technique and significance of treatment were compared by using Tukey’s (HSD) test at 5% probability level.  

...

Muhammad Qaisar Nawaz*, Khalil Ahmed, Ghulam Qadir, Muhammad Rizwan, Muhammad Faisal Nawaz and Muhammad Sarfraz 

...most cost-effective technique for turnip production under moderately salt-affected soils. 

...
Ibrahim Tapki1*, Huseyin Bahadir Ozalpaydin2, Nuran Tapki3, Mehmet Aslan4 and Muhammet Hanifi Selvi1
...seems to be a potential liquid feed additive that improves feed efficiency, health status, growth performance, and that reduces diarrhea incidents, hastens weaning age and minimizes dairy farm costs when used at the level of 100 mg/l.
...
Imdad Ullah Khan1, Shehla Gull Bokhari2, Mumtaz Ali Khan3, Ikramul Haq3*and Naimat Ullah Khan4
...ciate flap incision technique at the anal site.Finally, the urachal duct was identified at the apex of the urinary bladder, and excised after ligating itclose to the bladder wall. The calf recovered uneventfully after a post-operative antibiotic and antipyretic cover for 7 days.
...
R. Yasmeen1*, S. Shaheen1, B.N. Khan2, S.S. Bokhari1, U. Rafi1 and A.W. Qurashi1
...ve and non-invasive techniques are used. In the present study, enclosure of Axis axis commonly known as spotted dear was selected from Lahore and Bahawalpur zoo. Non-invasive technique was used to monitor heavy metal exposure and both biological (faecal, feed) and non-biological (water, soil) samples were collected. Soil was collected from different depths (6, 12, 18 inches) to study deposition patterns of metals. Sam...

Kalpana Polisetty1* and Kiran Kumar Paidipati2 

...pose of these three techniques is to capture the mutation point in time series data connected to wheat production during the period 1980-2016. Consequently, Sen’s slope estimator has been used to measure the magnitude of the trend for wheat production and their significances examined through Mann Kendall (M-K) test. The analysis provided a clear idea about the trends of wheat production over India. The results exhibit that all the states showed the raisi...

Muhammad Ramiz Murtaza1, Tahir Mehmood2*, Aziz Ahmad2 and Uzma Arshad Mughal3 

...ple random sampling technique was used to select 110 mango growers from 4 union councils of tehsil Ali Pur. To scrutinize the data double log model was renovated from Cobb-Dougles production function. Results showed that Benefit Cost Ratio (BCR) with and without intercropping was 1.72 and 2.48 respectively. Nearly 64 percent of the factors analyzed were affecting mango production and adjusted R2 was at 0.62. The whole goodness of fit model was enlightened by t...

Asif Yaseen1, Muhammad Israr2*, Allah Bux Khan1, Ahsan Javed3, Nafees Ahmad4 and Zia Ullah

...udy employed survey technique to collect data from 300 citrus farmers in Punjab, and multinomial treatments effects were used to analyze the data. The results indicated though participation in traditional marketing channels (with intermediaries) is still profitable, high value marketing channels appeared more attractive alternative, having been shown to offer increased net income to smallholder citrus growers. Findings showed the importance of production exper...

Tanweer Ahmed1*, Kashif Saeed Khan1, Zilakat Khan Malik1, Fazal Wahid1 and Muhammad Nadeem Iqbal2 

...Various econometric techniques have been applied to estimate the relationship among public spending on education, health, road length, defence, trade openness, worker remittances, total cropped area and economic growth. Pakistan’ economic growth rate fluctuated from 1972 to 2014. During this period for some years, the economic growth was high while it slowed down in some years. The study utilised Annual time series data from 1972 to 2014. Empirical evide...

Haidar Ali1*, Malik Muhammad Shafi1, Himayatullah Khan1 and Hamra Haidar

...ing random sampling technique for this study. Analysis showed that small farmers of developed villages perform more off-farm employment than small farmers of underdeveloped villages. This could be associated to the developed means of transport and communications, better education facilities, market facilities as well as availability of off-farm jobs locally. Furthermore, in developed villages average per month income of small farm households from farm output w...

Sanaullah1*, Urooba Pervaiz1, Shahid Ali2, Mohammad Fayaz2 and Aftab Khan

...ied random sampling technique. Data were collected with the help of a pre-tested and validated well-structured interview schedule. Analysis was carried through multiple regression and independent sample t-test. Results indicated that extension visits, involvement in agriculture, adoption of recommended farming practices, irrigation and HYVs had significant effect, while disease, fertilizer and pesticide had non-significant effect on maize yield. Nevertheless, ...
Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...using ultra performance liquid chomatography and mass spectrometric (UPLC-MS) analysis suggested the presence of a variety of bioactive compounds including totarol, kifunensine, talaromycin and clausine F. These results indicated that Kallar Kahar Lake harbors a significant diversity of actinobacterial strains which are able to produce a diverse range of biotechnologically and pharmaceutically potent compounds. Further studies related to the structure elucidat...
Muhammad Faheem Akbar1,*, Muhammad Abdul Haq2, Imtiaz Ahmad1, Vasileva Viliana3 and Amjad Sultan1
... using high performance liquid chromatography. First order degradation kinetics was fitted on this data and degradation rate constants and half-life were calculated. Conventional pesticides were found to be more persistent in the crop (Average half-life: 2.94, 4.06 and 2.74 days for imidacloprid, endosulfan and profenofos respectively) than bio pesticides (Average half-life: 3.87 and 1.81 days for spinosad and biosal respectively). The crops treated with bio p...
Muhammad Irfan Ullah1*, Muhammad Arshad1, Sajjad Ali2, Asad Abdullah1, Samina Khalid3, Hafiz Muhammad Aatif4, Syed Muhammad Ali Zahid1, Muhammad Afzal1 and Jaima Molina-Ochoa5
...mparable with other techniques like digital analysis by ImageJ software and leaf area meter devices. With these results, it is concluded that the BioLeaf application has resulted in an accurate leaf area measuring tool of foliar damage with minimum time and cost and can be used in various crops for damage assessment.

...
Jun Yan Bai*, Hong Deng Fan, You Bing Yang, Xu Wang, Heng Cao, Xue Yan Fu and Yu Qin Wang
... PCR and sequencing techniques. The results showed that large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep, hu sheep all have two mutation sites (C155T and C624T). For locus C155T, allele frequencies of C in large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep and hu sheep were 0.73, 0.83, 0.54, 0.69 and 0.68 respectively, which indicated that C was the dominant allele in five sheep population...

Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui

...ion is a phenomenal technique to serve rapid multiplication of vegetatively propagated plants through in vitro culture. This study was conducted to establish the optimal concentrations of plant growth regulators and sucrose for micropropagation of sugarcane. Sugarcane is cultivated through cane sets and therefore, multiplication of any new genotype through traditional practices requires interminable period of time. Eight sugarcane genotypes viz. NIA-2004,...
Daniel Cocan1, Vioara Mireşan1, Florentina Popescu1, Radu Constantinescu1, Aurelia Coroian1, Călin Laţiu1, Romulus Valeriu Flaviu Turcu3, Alexandru Ştefan Fărcăşanu3 and Cristian Martonos2,*
...redators. Using MRI techniques for anatomical investigations of venomous glands in Brown bullhead proved to be very precise both in identifying the shape and the topography of the venomous glands. Also by using MRI techniques we highlighted the complex defence mechanism that Brown bullhead has developed against its natural predators.
...
Boxin Dou, Ying Liu*, Yumeng Liu, Lili Fan, Yongqiang Ma, and Yanguo Shi
...ive chromatographic techniques successively, the acidic hydrolysates of RB proteins were fractionated and the new ACE inhibitory triplet was isolated and identified. The amino acid sequence of the ACEI was identified as Ile-Thr-Leu or Leu-Thr-Ile. In vitro, ACE inhibition assays showed that the IC50 value of the peptide was 0.0118 mg.ml-1. The inhibitory activity of the peptide slightly increased after incubation with ga...

Amit Sharma1* and Pankaj Sood2 

...d, semen collection technique, collection season, extender composition, type of cryoprotectant used, cooling rate, equilibration time, freezing and thawing rate. All these factors should be given due credit for achieving better post-thaw post thaw quality of semen to be utilized in different advanced reproductive techniques. 

...
Muhammad Rizwan Ashraf1, Asif Nadeem1,2, Eric Nelson Smith3, Maryam Javed1, Utpal Smart3, Tahir Yaqub4, Abu Saeed Hashmi1 and Panupong Thammachoti3
...tus). Molecular techniques have made it easy to elucidate phylogenetic relationships and evolutionary histories of different groups of organisms. This study is the first attempt to find the phylogenetic relationship and diversity through mitochondrial genes in saw-scaled viper from Pakistan. Tail tip biopsies of Saw-scaled vipers were used for amplification of mitochondrial genes fragments (ND4, 16S rRNA and 12S rRNA) through Polymerase Chain Reaction (PCR...

Arjumand Nizami1, Muhammad Zulfiqar2*, Jawad Ali1, Naushad Khan2 and Imran Sheikh

...her water efficient technique known as The Alternate Wetting and Drying (AWD) technology evolved by International Rice Research Institute (IRRI). AWD intervention in conjunction with laser land leveling is made through Water Productivity (WAPRO) project in Muridke tehsil of district Sheikhupura Punjab Pakistan. This study has been conducted with the aim to determine the irrigation water use efficiency at rice farms as a result of adopting AWD and laser land le...

Muhammad Zeeshan Nazar*, Ayyan Umer, Zahid Mahmood Sarwar, Faiz Ullah Faiz and Sikandar Hussain 

...cid body and haemolymph liquid impelled leading to mortality. Provide the required temperature and humidity with appropriate hygienic condition to B. mori are advised to produce healthy silk. 

...
Dongping Liu1, Guogang Zhang1, Chao Wang2, Baoping Qing2 and Jun Lu1,*
...ia, but since 1981 its unique population has been confined to a narrow band in transborder area of Yangxian, Chenggu and Xixiang County of Shaanxi Province, Central China, on the south slope of the Qinling Mountains. During 2004-2005, 23 crested ibises were experimentally reintroduced to an isolated basin in Qinling Mountains, where they and their offspring exhibited breeding variation never observed in wild population in the following 9 breeding seasons. Cres...

Aftab Khan1, Shahid Ali1*, Asim Khan1, Muhammad Waqas1 and Sufyan Ullah Khan2 

...ampling. Parametric technique of stochastic frontier production function was estimated to model output of maize growers. Results of stochastic frontier model revealed that tractor hours, labor and FYM positively and significantly affected yield of maize. Average technical efficiency was predicted to be 0.68 ranging from 0.31 to 0.91; implies that an average maize farm can increase yield by 33 percent with existing technology. Technical inefficiency effect mode...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1  
...tegies to protect this unique genetic resource of Pakistan.
...
Sikander Ali* and S. Wajiha Khalid
...gal exo-inulinase under liquid culture. Different fungal cultures were isolated and collected from various localities of Lahore and Murree (Punjab, Pakistan). Screening of these cultures was carried out in chemically defined medium i.e., 0.5 g/l (NH4)2SO4, 3 g/l KH2PO4, 1.5 g/l NaNO3, 0.01 g/l MgSO4.7H2O and 3 g/l inulin at pH 7. Kinetic parameters for comparing product yie...
Saeed Fatima1, Khalid Javed Iqbal1, Usman Atique2,3*, Arshad Javid4, Noor Khan2, Sonia Iqbal2, Hamid Majeed5, Hamda Azmat2, Bakhat Yawar Ali Khan6, Irfan7, Muhammad Tausif Shahid1, Gulnaz Afzal1
... might be due to their unique feeding mood. Cadmium presence ranged between 0.04 – 1.12 ppm while As and Hg fluctuated between 0.01 – 0.04 and 0.001 - 0.18 ppm, respectively. Conversely, higher bioaccumulation was detected in gills rather than the muscle tissues that evidently indicated gradual water quality degradation conceiving higher metal concentrations in the environment. Similarly, a higher concentration of Cd was observed in water (0.78 ppm...

Gulnaz Hameed1, Abdul Saboor1, Khuram Nawaz Sadozai2*, Ghaffar Ali2, Dawood Jan2 and Mansoor Rasheed

...Multistage sampling technique was used to draw a sample of 540 respondents from the universe comprising both registered and nonregistered members of AJKCDP. Primary data were collected through interview schedule. Foster Greer and Thorbecke measure was used to calculate incidence of poverty which is then used to model the probability expressions with the help of Logit to investigate the correlates of poverty in the research area. Poverty was found at below for ...
Urosevic Milivoje1, Matejevic Milosava2*, Drobnjak Darko1 and Ozkanal Umit3
...e;dération Cynologique Internationale (FCI). Research and analysis of morphometric parameters are required in order to write an official breed standard and recognize this breed. Previous research on this sighthound breed has shown only average values of individual morphological parameters and differences in them depending on gender, age or place of origin. However, the premise of this research is that there are several different varieties of Tazi, d...

Fazal Haq*, Asia Parveen, Safdar Hussain and Arshad Hussain 

...and random sampling technique is used to select the sample. Self made questionnaires, filled through interviewed process from the random selected farmers were used as a tool for collecting primary data. The raw data is tabulated and analyzed using Microsoft Excel. The analyzed data is used to develop the mathematical model. Simplex method is applied to find the optimal solution of the model, which showed 10.18% increase in the net revenue per year.  

...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...e straws were frozen in liquid nitrogen vapour using a computer controlled automatic freezer. After one day, straws were thawed in a water bath at 38ºC for 20 sec and then were examined. Semen analysis included viability, DNA defragmentation and motility of spermatozoa were being estimated. The addition of 2μg / mL of the GnRH analogue significantly (P <0.05) improved the percentage of live spermatozoa compared to the control group. Samples w...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
...ical, and molecular techniques. Virulence and pathogenesis of the disease were determined by intraperitoneal injection of the etiological agent to the healthy A. sinensis. Physiological and 16s rRNA molecular analysis identified Streptococcus iniae as the causative agent of the disease outbreak. The bacteria has a unique biochemical profile compared with most of the previously isolated strains. Antimicrobi...

Muhammad Ali1*, Norsida Man2, Farrah Melissa Muharam2 and Siti Zobidah Omar

...ge cluster sampling technique. Multiple linear regression through SPSS was administered for statistical procedure. The findings confirmed that the theory (TPB) was statistically feasible to predict behavioral intention of farmers to use ICTs for agricultural risk management. The results further reveal that attitude was one of the most influenced of intention, which was followed by perceived behavioral control and lastly the subjective norms. Thus, using TPB in...

Shah Nawaz Khuhro1*, Irshad Ali Junejo1, Muhammad Haroon Hullio1, Sultan Ahmed Maitlo2, Javeed Shabir Daar3 and Shahjahan Rajput

...condition at Shaheed Zulfiqar Ali Bhutto Agricultural College Dokri, Sindh-Pakistan. Different coloured sticky traps contains yellow, light green colour, green colour and pink colours were used to monitor the movement of whitefly and the number of whitefly catches at traps on different observations on brinjal. Results of field trials confirmed that yellow sticky trap attracted more number of whiteflies as compared to the green, pink and light green, so yellow ...

Olotu Titilayo Mabel*, Adeosun Idowu Jesulayomi, Kaka Mary Oluwatosin and Oladipo Elijah Kolawole 

...d the pol gene is very unique encoding the enzymes for replication. Our objective is to describe the relatedness of these HIV-1 pol genes with each other and its antiviral drug susceptibility which will provide more information on its sensitivity and resistivity to each antiviral drug. Seventeen partial genome sequences of HIV-1 Pol gene from some West African countries were retrieved from NCBI database. Evolutionary relationship was determined by Multiple seq...

Khalid Khan1, Marguerite Wotto2, Saima Liaqat3, Gulawar Khan1, Balach Rasheed1, Sara Rafiq4 and Guo Xiangyu5* 

...age random sampling technique was used for selection of the farmers. The Cobb-Douglas production function was used to estimate the technical efficiency and performance of the farmers. The study results disclosed that all explanatory variables have a positive impact on the farmers’ technical efficiency apart from pesticides. Additionally, the average technical performance of the farmer was 85% which shows that on average a farmer can spur the tomato produ...

 Salman Khan, Irfan Ullah*, Shahid Ali and Murtaza

...age random sampling technique was used to collect data from 100 respondents during 2018. A Cobb-Douglas type production function was used to approximate effect of determinants on potato yield. Major shares of the input cost per acre incurred in the study area were seed (Rs. 18720), followed by land rent (Rs.15890) and labors having an estimated cost of Rs. 14965 respectively. The analysis showed that on average per acre yield of potato was recorded as 4,953.35...

 Abdus Salam* and Muhammad Zafarullah Khan

...ulti-stage sampling technique, population for the current study, 3 zones from 5 were purposively selected on the basis of their different agricultural condition. Selection of sample size was made using Yamane formula. The number of respondents as per formula was confined to 188 respondents in district Mansehra, 153 respondents in district Swabi and 59 respondents from district Karak, where data were collected randomly. Five point Likert scale was used to recor...

Kajol, A. H. Bhat, Aasha and A. K. Chaubey

Biochemical and molecular characterization of Photorhabdus akhurstii associated with Heterorhabditis indica from Meerut, India
...a by insect baiting technique. The isolate was identified as Heterorhabditis indica based on morpho-taxometrical and molecular analyses. The present populations were identical to the original description; however, the size of the 3rd stages was longer than the topotype populations. Analysis of ITS-rDNA sequences showed 1 nucleotide base pair difference in aligned data with type description, however, no nucleotide base pair difference was seen in the D2D3 domai...

Sahib Khan1, Muneeza Wahid2, Muhammad Abeer Irfan1, Muhammad Naeem3, Asma Gul4, Muhammad Haseeb Zafar2

ROW LEVEL IMAGE FORGERY DETECTION TECHNIQUE USING EMBEDDED DIGITAL SIGNATURES
...is presenting a new technique to detect any changes made in digital images. This technique ensures the integrity of digital image at row level and using embedded digital signatures. Using message digest 5 algorithm, digital signature is generated from selected pixels of each row using selected pixels for and embedded in the least significant bits of selected pixel of the corre- sponding row of digital image. The proposed tec...

 Gulzar Mehmood1*, M. Zahid Khan1, Haseeb Ur Rahman1, and Sohail Abbas2

AN EFFICIENT AND SECURE SESSION KEY ESTABLISHMENT SCHEME FOR HEALTH-CARE APPLICATIONS IN WIRELESS BODY AREA NETWORKS
...esearch. WBAN has many unique applications especially in medical and health-care. This kind of network
provides support in on-the-spot decision making and therapeutic treatments. However, one of the main issues with
WBAN in medical applications is security and patients’ information privacy. In such applications ensuring human
vital sign privacy and security is very important. To address this important issue, in this paper we propose ...

 Tajwali Khan1*, Ihtzaz Qamar1, Feroz Shah2, Kareem Akhtar2, Rehman Akhtar3

MODEL FOR FUEL DROPLET EVAPORATION IN COMBUSTION CHAMBER OF LIQUID PROPELLANT ROCKET ENGINES
...bsp;Complete burning of liquid propellants droplets is very important to avoid combustion instability and to get higher
specific impulse from liquid rocket engines. Combustion takes place in gaseous phase and reaction is fast. So, the
time consumed by a droplet for complete burning is the time taken by a droplet to get evaporated. An analytical
mono component fuel droplet evaporation model is developed a...

Muhammad Alam Zeb Khan1*, Muhammad Ali Kamran1, Khan Muhammad2, Khizar Azam1 

IDENTIFICATION OF THE OPTIMAL OPERATING PARAMETERS OF LOCALLY AVAILABLE COAL FOR USE IN PAKISTANI INDUSTRIES
...l
combustion technique. Effect of mesh size, primary and secondary air velocity at various air fuel ratios was investigated
to quantify the combustion performance by measuring the furnace temperature. Mesh size 200 was found to
be readily combustible within the operating ranges, while tight control of the operating parameters was required
to combust coal at mesh size 150. Optimum ranges of primary air and Air-Fuel Ratio are suggeste...

Riaz Muhammad1*, Naseer Ahmeda, Abdul Basit2, Rizwan Alim Mufti2 

EXPERIMENTAL INVESTIGATION OF FRACTURE TOUGHNESS, HARDNESS AND LOAD-INDENTATION DEPTH RESPONSE OF TI-64 USING VICKERS INDENTATION TECHNIQUE
...4 using indentation technique which is easy and fast experimental techniques.
For this purpose Vickers-indentation technique is employed to report the hardness followed by fracture toughness
evaluation of the studied alloy using indentation energy model. The indentation method resulted a fracture toughness
of the studied alloy with 14.5% when compared to the results ob...

Sahib Khan1, Muhammad Naeem2, Tawab Khan3, Nasir Ahmad4* 

4LSB BASED DATA HIDING IN COMPLEX REGION OF DIGITAL IMAGES AND ITS EFFECTS ON EDGES AND HISTOGRAM
... 4LSB steganography technique has been found very efficient, creating no significant effect on edges
and undetectable histogram differences. Which prove this technique immune to histogram difference steganalysis. The
quality of the edges is preserved and a PNSR of greater than 74dB has been observed for different cover images
for the true edge based 4LSB steganography. The quality of stego images, edges ...

 M. Ihsan Ul Haq1, Ahmad Hussain2*, Farzana Yasmeen3, Shafiq R. Qureshi4

LIFE CYCLE COSTING AND PAYBACK PERIOD EVALUATION OF A SOLAR THERMAL DESALINATION SYSTEM
...e best desalination techniques in the developing stages. Solar energy, passive vacuum and recovery of
latent heat of condensation make this system a sustainable option for desalination. In this paper, economic analysis
of the solar thermal desalination system of saline water is presented. The unit cost of desalinated water is found to
be US$ 0.0147 per litre. The energy and emission payback (EEP) period for vacuum chamber and solar collect...

Naseem Ahmad1, Sahar Noor1, Rehman Akhtar1*, Misbah Ullah1, Khizar Azam2 

PERFORMANCE IMPROVEMENT OF COMPRESSOR TO RECOVER FLARE GAS USING DESIGN OF EXPERIMENT TECHNIQUES
...experimental design techniques to recuperate large
quantity of flare gas with the help of a new proposed plant process. Some factors responsible for disturbing routine
of compressor are categorized and pertinent information is togethered from various resources. The information is
thoroughly studied to enhance factors performance and the factors were set up according to the modified compressor
compression system. The suggested equipp...

Zahooruddin1*, Muhammad Altaf1, Ayaz Ahmad1, Farooq Alam1 

GRADIENT ASCENT INDEPENDENT COMPONENT ANALYSIS ALGORITHM FOR TELECOMMUNICATION SIGNALS
...A (AS-GAICA)
technique for varying block length that can also controls the maximum number of iterations adaptively. The performance
of the proposed technique is compared with Fast-ICA and Optimum Block Adaptation ICA (OBAICA) for
telecommunication signals. Simulation results show that the proposed scheme outperforms the Fast-ICA and OBAICA
algorithms. 

...

 Waqas Javaid1*, Adnan Tariq1, Iftikhar Hussain2, Sahar Noor2

AN EVOLUTIONARY APPROACH FOR CMS DESIGN
...e
(AI) based techniques. The main advantage of such approaches is their ability to handle the CFP effectively both in
terms of accuracy and computational effort. Following the same trend an evolutionary algorithm has been developed
during this research by combining Standard Genetic Algorithm with a very effective Local Search Heuristic (LSH).
The results show that it is efficient both in terms accuracy and speed of convergence (CPU ...

Razaullah1*, Iftikhar Hussain1 

A TWO-WAREHOUSE SUPPLY CHAIN NETWORK DESIGN AND OPTIMIZATION WITH CROSS-ROUTE COSTS AND BUDGET, MAXIMUM FLOW AND CAPACITY CONSTRAINTS
... chain optimization techniques for modelling the behavior of manufacturing supply chains have been used
for long in order to allow better planning, minimize total cost and improve efficiency. In this paper, a systematic
approach is presented for the facility placement, optimal production planning and product transportation across
network arcs. An optimization formulation is developed for the determination of production size, locations of n...

Nabeel Maqsood1,2*, Afzal Khan1, Muhammad Khalid Alamgir2, Shaukat Ali Shah1, Muhammad Fahad3 

PTFE THIN FILM COATING ON 316L STAINLESS STEEL FOR CORROSION PROTECTION IN ACIDIC ENVIRONMENT
... SS by spin coating technique to modify their corrosion
resistance in acidic media containing Hydrochloric acid (HCL). The anticorrosion property of the 316L SS and PTFE
coating was studied in 40% HCL by electrochemical corrosion test and potentiodynamic polarization curves at room
temperature and compared. The morphology of uncoated and coated substrates were examined by scanning electron
microscopy (SEM) while the compositional an...

Murad Ullah Khan1*, S. Zuhra2, M. Alam3, R. Nawaz4 

SOLUTION TO BERMAN’S MODEL OF VISCOUS FLOW IN POROUS CHANNEL BY OPTIMAL HOMOTOPY ASYMPTOTIC METHOD
...Homotopy asymptotic technique
(OHAM). Reynolds number is based on Suction or injection through the wall, so for different values of Reynolds, we
obtained different types of semi-analytic solutions by OHAM. 

...

Misbah Ullah1, Rehman Akhtar1, Sikandar Bilal Khattak1, Khawar Naeem1 and Khizar Azam2 

FACTORS IDENTIFICATION FOR COAL BRIQUETTES MANUFACTURING USING STATISTICAL EXPERIMENTAL DESIGN APPROACH
...ions.

...

Rafiullah Khan1, Waseem ur Rahman1, Misbah Ullah2, Kamran Afaq3, Muhammad Amjad1, Sakhi jan1 

AGE EFFECT ON THE MECHANICAL PROPERTIES OF HIP JOINT BONE: AN EXPERIMENTAL INVESTIGATION
...g and measurements) techniques. The
test result revealed reduction in mechanical properties of hip joint bone with the age. 

...

Shabana Rafique1, Shahnaz Parveen Khattak1, Tanveer Hussain2, Faiza Tauqeer1, Bashir Ahmad3 

EVALUATION OF FLEXURAL RIGIDITY AND ABRASION RESISTANCE OF POST AND META-FINISHED PIGMENT DYED P/C FABRICS
...ation of fluorine based liquid water repellent finish (NUVAFD)
at high concentration well resisted the abrasion rubs. The optimized formulation, 50g/L of polyurethane had
improved the abrasion resistance of fabrics. Generally, the finishing of polyester/cotton fabrics with Pekoflam OP flame
retardant and water repellent chemicals induced resistance against heavy brunt of abrasive action and a desirable
flexural rigidity in fabrics. ...

Asif Ali Khan*, Laiq Hassan*, Salim Ullah* 

OPEN MP-BASED PARALLEL AND SCALABLE GENETIC SEQUENCE ALIGNMENT
...stributed computing techniques in order to speed up this process. In this paper, we discuss and
evaluate an OpenMP based implementation of SW algorithm. All the experiments have been performed on a Linux
based multi-core machine thereby reducing the overall complexity of the SW algorithm from quadratic to linear. The
results obtained with various input sequences demonstrate that the parallel version of the SW algorithm runs 2.63
tim...

Suhaib*, Shahid Maqsood*, Sikandar Bilal Khattak*, Misbah Ullah*, Rehman Akhtar*, Rashid Nawaz*, Iftikhar Hussain*, Abdul Shakoor**, Khizar Azam** 

SINGLE FACILITY LOCATION SELECTIONPROBLEM FOR A PAKISTAN BASED ICE CREAM COMPANY: A CASE STUDY
...cation optimization techniques
provide optimum or near optimumsolution. In this paper, an optimization technique median method is applied to a
real world problem of a multinational ice cream company’s factory located in Lahore, Punjab (province) Pakistan,
with an objective of minimum overall cost between a warehouse and destinations. The company wanted to locate the
warehouse in Khyber Pakht...

Hassan Nasir*, Rashid Rehan,** Rizwan Younis,*** Fazal Mabood,**** Salman Saeed,**, Muhammad Sagheer Aslam** 

MACHINERY SELECTION FOR DEMOLITION AND RECYCLING OF PAVEMENTS: A SIMULATION-BASED TOOL
... This paper presents a unique simulation tool based on the
system dynamics methodology to help the construction manager in investigating the cost and duration implications of
various combinations of machinery items. Utility of the developed model is illustrated with the help of a case study
involving demolition and recycling of a flexible pavement. 

...

Muhammad Shahid1, Adiqa Kiani2* and Mahmood Shah3

Livestock Tackles the Major Sector Fluctuations in Agrarian Sector and Raises Economic Performance: A Study of Pakistan
... (ARDL) econometric technique was applied to conclude empirical results. Further, cumulative sum (CUSUM) and CUSUM square test was used to check the stability and structural breaks of the model. The results of research show the statistical significant and positive impact of livestock on economic performance while inflation effect on economic performance is insignificant. I have reviewed a lot of economic literature but not found single one study of this nature...

Muhammad Asad Ali, Mirza Jahanzaib, Muhammad Ahsan Ul Haq 

ANALYZING THE EFFECT OF SQUEEZE CASTING PROCESS PARAMETERS ON MECHANICAL PROPERTIES OF OVERCAST AL-ALLOY JOINT USING RSM
...nts produced by casting liquid 2024 wrought aluminium alloy onto the solid 2024 wrought aluminium alloy inserts using squeeze casting process. The quality of overcast joints fabricated via squeeze casting depend on mechanical properties such as ultimate tensile strength and yield strength. Mechanical properties depends upon the input casting parameters named as squeeze pressure, pressure duration and melt temperature. Response surface methodology (RSM) was emp...

 Mahmood Alam Khan, Afzal Munir, Muahmmad Shahzad Khattak, Daulat Khan, Muhammad Ajmal

GROUNDWATER RECHARGE QUANTIFICATION FROM RAINFALL IN PESHAWAR DISTRICT-KHYBER PAKHTUNKHWA
... water conservation techniques are recommended to increase water recharge in the study area.  

...

Muhammad Shahid1, Adiqa Kiani2* and Mahmood Shah3

Livestock Tackles the Major Sector Fluctuations in Agrarian Sector and Raises Economic Performance: A Study of Pakistan
... (ARDL) econometric technique was applied to conclude empirical results. Further, cumulative sum (CUSUM) and CUSUM square test was used to check the stability and structural breaks of the model. The results of research show the statistical significant and positive impact of livestock on economic performance while inflation effect on economic performance is insignificant. I have reviewed a lot of economic literature but not found single one study of this nature...

Hina Fatima1*, Sania Shaheen2, Lal Khan Almas3 and Sehrish Haroon4

Profit Efficiency among Transplanting and Direct Seeded Rice Producers (Case Study of Certain Rice Growing Areas of Province Punjab, Pakistan)
...ices and production techniques. Moreover, the results also demonstrated that farmer’s education, age, farming experience, extension service facility and land tenancy considerably effects profit efficiency of rice producers. Therefore, the efficiency of rice farmers can be improved through increase in the number of educated people in farming system, and the enrichment of extension services in the rural areas.

...

Sajjad Haydar*, Zunaira Asif*, A. A. Bhatti**, Obaidullah Nadeem***, Ghulam Hussain*, Nadeem Abbas*

EFFICIENCY OF WASTEWATER TREATMENT PLANT IN THE PUNJAB TANNERY SECTOR: A CASE STUDY OF DADA TANNERY
...on of
Mixed Liquor Suspended Solids (MLSS) in aeration tank and low Phosphorous concentration in wastewater. NEQS
limits for BOD and COD could be met by adding Phosphorous and increasing the MLSS in aeration tank.
...

Ihsan Rabbi*, Sehat Ullah**, Aftab Alam**

MARKER BASED TRACKING IN AUGMENTED REALITY APPLICATIONS USING ARTOOLKIT: A CASE STUDY
...stems, marker-based technique is widely used approach to track the video
camera pose. In this paper, we present an analysis of marker-based tracking using ARToolKit. We investigate the
effect of marker size, distance between markers and camera, the speed of marker with respect to camera, relationship
between marker size and distance, the brightness and contrast level of a camera on tracking a single marker.
Exper...

 Sikandar Afridi*, Muhammad Irfan Khattak**, Nasim Ullah***, Gulzar Ahmad**, Muhammad Shafi**

PERFORMANCE ENHANCEMENT OF FACE RECOGNITION SYSTEM USING PRINCIPAL COMPONENT ANALYSIS MERGED WITH DISCRETE WAVELET TRANSFORMS
...nhanced by proposed technique titled as PCA merged with Discrete

Wavelet Transform (DWT) instead of using the conventional PCA technique. In this technique to reduce the computational
complexity of traditional PCA the size of the image is first reduced by taking the DWT of it. After applying
the DWT the facial features of the image are extracted by calcula...

Atta Ur Rehman, Laiq Hassan, Nasir Ahmad, Kashif Ahmad, Shakirullah

COMPARATIVE STUDY OF SUPPORT VECTOR MACHINE AND HAMMING DISTANCE USED FOR IRIS RECOGNITION
...nown classification techniques of iris patterns, along with detailed

description of some preprocessing steps. In preprocessing stage, Circular Hough Transform and Canny Edge
Detector are employed for iris segmentation, while for iris normalization and feature extraction, the Rubber Sheet
Model and one-dimensional (1-D) Log-Gabor Filter are used respectively. Finally for classification/matching of iris
patterns, Ham...

 Muhammad Asad Ali 1*, Mirza Jahanzaib1, Muhammad Ahsan Ul Haq1

ANALYZING THE EFFECT OF SQUEEZE CASTING PROCESS PARAMETERS ON MECHANICAL PROPERTIES OF OVERCAST ALUMINIUM-ALLOY JOINT USING RESPONSE SURFACE METHODOLOGY
...nts produced by casting liquid 2024 wrought

aluminium alloy onto the solid 2024 wrought aluminium alloy inserts using squeeze casting process. The quality
of overcast joints fabricated via squeeze casting depend on mechanical properties such as ultimate tensile strength
and yield strength. Mechanical properties depends upon the input casting parameters named as squeeze pressure,
pressure duration and melt temperatu...

 Mahmood Alam Khan1*, Afzal Munir1, Muhammad Shahzad Khattak1, Daulat Khan1, Muhammad Ajmal1

GROUNDWATER RECHARGE QUANTIFICATION FROM RAINFALL IN PESHAWAR DISTRICT-KHYBER PAKHTUNKHWA
... water conservation techniques are recommended
to increase water recharge in the study area.
...

 Sajjad Hussain1*, Nisar Mohammad1, Zahid Ur Rehman1, Noor Mohammad1, Ishaq Ahmad1,

Naseer Muhammad Khan2 and Salim Raza1
EXTRACTION OF GOLD AND SILVER BY AGITATION CYANIDATION PROCESS FROM COMPLEX ORE OF SHOUGHUR AREA CHITRAL, KYBER PAKHTUNKHWA PAKISTAN
...ersatile processing technique used for extraction of gold utilizing

different cyanidation parameters likes grinding time, pH, cyanidation time, sodium cyanide (NaCN) and lime CaO..
The efficiency of cyanidation process depends on dosage of each parameter. In present research work the extraction
of gold and silver by cyanidation process from shoughur area of district Chitral, Khyber Pakhtunkhwa was carried
out at la...

Nisar Mohammad*, Mohammad Mansoor Khan* Noor Mohammad*

CHARACTERIZATION OF TALC-CARBONATE ROCK OF MINGORA EMERALD MINES, DISTRICT SWAT, KHYBER PAKHTUNKHWA, PAKISTAN
...mmercial processing technique for the said talc carbonate rocks. Thorough investigations were conducted using SEM-EDX X-Rays Diffraction (XRD) , Thermal Gravimetri...

 Fcroz Shah*~ Mohammad A. Irfan**, Rizwan Gul*~ Abdul Shakoor*

ALGORITHM DEVELOPMENT AND IMPLEMENTATION FOR NUMERICAL DETECTION OF HOT SPOTS IN CASTING GEOMETRY
...-asian: normal;">technique is sensitive to the itifluence ...

 Muhammad Khurram Ali*, Raft Javed Qureshi**, Mirza Jahanzaib***

SELECTING FACILITY LOCATION USING HYBRID METHODOLOGIES IN GLOBAL PERSPECTIVE: A CASE OF CEMENT PLANT
...ferent optimization techniques. Therefore such factors were explored and prioritized from available literature and by recommendations ofpanel ofexperts followed by mathematical modelling of the said problem in four standard methodologiesincluding the one presented in another 

Farid Ullah Khan*, Sheraz Ali Khan*

A SMART STARTUP AND SHUTDOWN SYSTEM FOR DOMESTIC ELECTRIC POWER GENERATORS
...rent fUels i.e. Petrol, Liquefied Petroleum Gas and Natural gas.
...

Nisar Mohammad٭, Mohammad Mansoor Khan٭, Noor Mohammad٭

EXTRACTION OF HIGH QUALITY TALC FROM TALC-CARBONATE ROCK OF MINGORA EMERALD MINES BY FLOTATION AND LEACHING
...ck and to develop a technique for up gradation of indigenous noncommercial talc resources from other locations. These rocks host emerald crystals and the debris are dumped after excavation and extraction of emerald crystals. Controll of environmental threat caused by these rock dumps is an additional benefit of the research. Two step froth flotation process with AF65 (Poly propylene Glycol, Cyanamid) as frother, Sodium hexa metaphosphate as depressant for...

Farid Ullah Khan*

ACTUATION ENHANCEMENT OF A MICRO BIMORPH THERMAL ACTUATOR
...r micro fabrication techniques such Pulsed Laser Deposition (PLD), Laser Ablation (LA) and Micro electro-discharge machining (μEDM). Different designs of bimorph thermally actuated digital micro-mirror devices (DMDs) are also fabricated by the same generic fabrication process in order to show the practical utility of this research in the field of MEMS. The DMDs actuated by BTAs, are easy to fabricate and can scan through larger angles as compared ...

Muhammad Waseem*, Irshad Ahmad**, Rawid Khan**, Bashir Alam**, Khan Shahzada**, Muhammad Javed**

STABILITY ANALYSIS OF SOIL SLOPE IN SWABI, PAKISTAN
...lts, ground anchors technique was found to be a suitable method for slope stability in Swabi.
...

Azam Khan*, Irshad Ahmad**

IN-PLACE ANALYSIS OF JACKET OFFSHORE STRUCTURE UNDER STORM CONDITIONS
...bsp;sub-structuring technique, to model the whole structure. Appropriate dead load, live loads and quasi-static action of 100 yr storm have been applied to the structure. The structure-pile interaction analysis has been performed using SPLINTER, a component of the ANSYS ASAS program suite. The platform is subjected to design action, i.e combination of permanent and variable actions, and the basic Integrity checks including member strength, stability ...

Gulzar Ahmad*, Sahibzada Faheem*, Amjad Ullah*, Waqar Shah*, S.M. Majid Ashraf*

COMPARATIVE STUDY OF RELAY PROTOCOLS USING SIMULATION
... to compare various techniques used in cooperative relaying protocols. The performance is evaluated in terms of average symbol error under various ranges of signal-to-noise ratio (SNR) probabilities. A three node system i.e. source, relay and destination, placed on the edges of equilateral triangle, has been proposed using Maximal ratio Combiner (MRC) method by combining signals from source and relay at destination point. The transmission p...

Muhammad Naeem*, Sohail Asghar**

A REVIEW OF FEATURE SELECTION TECHNIQUES IN STRUCTURE LEARNING
... structure learning technique in Bayesian belief network. We have come up with some useful findings for overall computationally efficient approach among eleven evaluators. This analysis is useful in inducing better structure from the given dataset in imparting improved performance metric for Bayesian belief network.
...

Sajad Ali1*, Jangraiz Khan2, Arshadullah Jadoon3, Muhammad Riaz4 and Abid Khan1

Evaluation of Farmers Socioeconomic Characteristics Influencing Tomato Output in District Peshawar, Khyber Pakhtunkhwa, Pakistan
...ulti-stage-sampling technique and interview method were adopted for data collection. A sample of 160 tomato (Kharif) growers was drawn from 16 villages of district Peshawar. Chi-Square and Pearson Rank correlation tests were employed to find out the association and correlation between level of productivity and socio-economic characteristics of tomato growers such as education level, work involvement, family size, farming experience, age, and source of income a...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...tic random sampling technique. The response rate was 60%. Hence, the sample of 120 respondents was used for final analysis and description of the results. The instrument’s validity and reliability were checked before data collection. In-person interviews were conducted by the researcher. The collected data were analyzed by Statistical Package for Social Sciences (SPSS). Based on the results, it is concluded that majority of the farmers were not willing t...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...tic random sampling technique. The response rate was 60%. Hence, the sample of 120 respondents was used for final analysis and description of the results. The instrument’s validity and reliability were checked before data collection. In-person interviews were conducted by the researcher. The collected data were analyzed by Statistical Package for Social Sciences (SPSS). Based on the results, it is concluded that majority of the farmers were not willing t...

Feroza Arshad*, Aftab Ahmed Memon**, Muhammad Aslam Uqaili** and Arshad Habib Malik*

SYNTHESIS OF A NOVEL ROBUST INTELLIGENT CASCADED REACTIVITY CONTROLLER FOR CANDU REACTOR
...gent soft computing technique. The original reactivity controller of CANDU nuclear power plant is a networked controller implemented on a Programmable Logic Controller (PLC). The new NICRC is designed based on two Intelligent Distributed Cascaded Power Controller (IDCPC) and Intelligent Cascaded Moderator Level Controller (ICMLC) for moderator level control in CANDU reactor core. The IDCPC is composed of three neural sub-controllers while I...

Shahrukh Khalid* and Athar Mahboob**

A SURVEY ON AUTO-CONFIGURATION MECHANISMS FOR MOBILE AD-HOC NETWORKS (MANETS)
... communicate must have unique IP addresses. Auto-configuration refers to assignment of unique IP addresses to the nodes of MANETs. Due to characteristics of MANETs, assignment of IP addresses to nodes is a non-trivial problem and renders itself as an open issue and potential problem for research. A large number of mechanisms have been proposed for configuring MANET nodes. This paper presents a survey of l...

Abroon Jamal Qazi*, Afzal Khan*, Muhammad Tahir Khan*, Muhammad Ali Kamran* and Khizar Azam*

A NOVEL APPLICATION OF PARTICLE SWARM OPTIMIZATION TECHNIQUE IN SEMI-ACTIVE SUSPENSION SYSTEM CONTROL
...;and precision. The technique results in evaluation of appropriate gains that result in superior performance of the designed system. The models are compared on the basis of suspension displacement and tire displacement for ascertaining ride comfort and road handling attributes respectively.
...
Muhammad Shaheen
APPLICATION OF LABELLED K MEANS CLUSTERING FOR GIS CONTRACT AUTOMATION
...ised classification technique which is suitable for the dataset which do not have any class labels. The absence of labels constrained its application on different text data sets. Labelled K Means clustering generate labels for the clusters obtained from K Means Clustering which makes this technique more decisive for text data sets. A novel application of Labeled K Means Clustering for automation of technical part of GIS cont...

Behram Khan1, Amir Nawaz Khan2, Said Qasim3, Atta-ur Rahman4

ANALYSIS OF HUMAN PERCEPTIONS AND ADAPTATIONS TO FLASH FLOODS IN KHYBER AGENCY, FATA
...hic and statistical techniques were applied to process and analyze the data. As a result, the data were analytically discussed and presented in the form of tables, maps and plates. The analysis reveals that people of the area perceived about the recurrence of future flash floods during rainy season. Most of the people perceived the flash floods as it hit the study area with devastating impacts and the extent of flood damages. In spite of flood risk, the level ...

 Muhammad Shahzad1, Arif Iqbal Umar1, Syed Hamad Shirazi1, Muhammad Tariq Pervez2*, Zakir Khan1, Waqas Yousaf1

HCDP: HEPATITIS C DATA BANK OF PAKISTAN
..., Methylation and ubiquitination sites in the protein sequences, motif/signature sub-sequences pattern, visual appearance of protein/nucleotide sequences for analysis of different sites, visual representation of multiple sequence alignments using colour code along with motif finding/conserved region in the sequence and analysing of graphical structure of phylogenetic tree. With the help of Format converter/Fasta generator tool user can conv...

 Nashitah Alwaz1, Safdar Raza1*, Sadaqat Ali1

A COMMUNICATION-FREE SIGNAL GENERATING TECHNIQUE FOR REACTIVE POWER CORRECTION IN ISLANDED MICROGRID
...ever, these control techniques suffer badly due to the feeder impedances and uncontrollable power coupling issues. Many techniques have been proposed to share power accurately, most of them rely on communication
link. In this work a localized/communication free signal generating mechanism is proposed. The mechanism injects the transient reactive power in frequency droop char...

 Nisar Mohammad1, Mohammad Mansoor Khan1

RECOVERY OF TALC FROM TALC- CARBONATE SCHIST OF SWAT EMERALD MINES, NWFP, PAKISTAN
...ble processing technique to recover talc. A few processing tests using flotation, gravity separation and leaching methods were conducted to see the amenability of talc to enrichment by these techniques. Analysis of seven talcum powder products available in the local market including pharmaceutical grade talc was carried out for comparison with Swat raw- talc under study and to know the level of impurities...

Abdul Shakoor1, R.A. Sayed2, Khizar Azam2

KNOWLEDGE BASE TECHNOLOGIES AS A NEW PARADIGM IN DATA DRIVEN MANUFACTURING SYSTEM
...knowledge capturing techniques. The machining examples are used to demonstrate the implementation of the system and how it will provide a basis for knowledge sharing in PLM environment. The Manufacturing knowledge is captured in order to provide manufacturing consequences.
...

 Laiq Khan1, Sidra Mumtaz2, Kamran Javed3, S.W. Shah4, M.I. Khan Babar5, S.R. Akbar6

GENERATORS MAINTENANCE SCHEDULING USING HARMONY SEARCH ALGORITHM
... new meta-heuristic technique. It is used to solve multi-objective optimization problems. Generators maintenance schedules (GMS) play the most significant role for the economical and reliable operation of a power production system. This paper presents the application of harmony search (HS) algorithm for solving generators maintenance scheduling problem. HS algorithm is quite efficient, because, the convergence rate of this algorithm is very...

 Arshad Ali1, Hashim Nisar Hashmi2

TREATMENT FEASIBILITY OF THE BLEACHING EFFLUENT OBTAINED FROM NSSC PULP AND PAPER MILL IN A UASB REACTOR
...feasible and viable technique.
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Farah Mahmood1, Mohammad Inayatullah Babar1, Syed Waqar Shah1, Hamid Ullah1, Riaz Akbar Syed1

GPS ASSISTED HANDOFF IN OVERLAPPING IPv6 WLAN WITH MULTIPLE CoA
...eless networks and the unique features of mobile IPv6 it has become possible to utilize network services at remote location. However the handoff issues due to mobility can lead to serious delays. In this paper we present a unique approach for handoff when mobile node moves into a foreign network. We have utilized GPS (Global Positioning System) for gathering location information and multiple care of addre...

 Faizullah Mahar1, Syed Saad Azhar Ali1, Abid Karim1

DESIGN OF FIXED ORDER ROBUST CONTROLLER USING H∞ -NORM AND EVOLUTIONARY TECHNIQUES: COMPARISONS AND PERFORMANCE ANALYSIS
...use of evolutionary techniques (i.e. genetic algorithm and immune algorithm) for the designing of a low, fixed order robust controller. To check the effectiveness of the proposed approach, resulting controller parameters are evaluated for performance analysis via extensive simulations. Simulation results and performance comparisons demonstrate the efficiency of proposed approaches.
...

Sikander Hiyat1, Nazim Hussain1, Muhammad Ishaq Asif Rehmani2*, Muhammad Nasir Abbas2, Smana Raza3, Javed Shabbir Dar4 and Tauqeer Ahmad Yasir5 

...text-align: justify;">Mepiquat chloride, widely used growth regulator in cotton fields to increase crop yield. The present study was performed to investigate the effects of growth regulator (mepiquat chloride) and row spacings on growth and yield attributes of cotton. The experiment was conducted on randomized complete block design (RCBD) with split plot arrangement. Three levels of row spacing (25 cm, 50 cm, and 75 cm) were...

Zahid Hassan Tarar1, Muhammad Salik Ali Khan2*, Shahzada Munawar Mehdi3, Raza Salim4, Irfan Ahmad Saleem1, Saima Nazar5, Munir David2, Tahir Majeed6, Muhammad Sufyan Mughal7, Muhammad Saleem8, Umer Iqbal9, Muhammad Mazhar Iqbal10 and Muhammad Khalid Shaheen

...he ordinary kriging technique was used to assess the micronutrient status at non-sampling sites and research area was classified into low, marginal, and adequate categories according to plant available micronutrient status. Digital maps thus developed using Quantum Geographic Information System (QGIS) would help to apply micronutrients more precisely and site-specifically.  

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Erum Bughio1, Ahmed Sultan Jatoi1*, Jibran Hussain2, Muhammad Hayat Jaspal3, Shahid Mehmood2, Hafiz Muhammad Ishaq4, Reema Bughio5 and Athar Mahmud2

Comparative Study of Carcass Traits in Four Strains of Japanese Quail (Coturnix japonica) at 3 to 6 Weeks of Age
...e calculated. ANOVA technique was used for data analysis and DMR Test for comparison of means. Significant (p<0.05) differences were observed in mean slaughter and liver weight (g) in quail birds. Significant (p<0.05) differences were noted in keel length (cm) at week 3, 5 and 6 and keel angle at week-3. A significant (p<0.05) effect was observed in mean heart weight at week 3 and 6, gizzard weight at week 4, 5 and 6 and intestinal length (cm) at week...

Oluwatoyin Olagunju1* and Luqman Abiodun Akinbile2

Farmers Perceptions of the Effect of Rural Transportation Systems on Farming Income in Ondo State, Nigeria
...age random sampling technique. Data used for the study were analyzed using descriptive statistics and inferential statistics such as chi-square and Pearson Product Moment Correlation (PPMC). The study revealed that farmers perceived the quality of transportation system causes a reduction in their income. Gender (χ2=6.472) and marital status (χ2=9.745) positively influenced income generated by farmers at p<0.05. Also, there was a significant relation...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...ital camera and PCR technique, respectively. For molecular identification, DNA was extracted through CTAB method and polymerase chain reaction was performed using mitochondrial cytochrome oxidase I (COI) gene based primers. Gel electrophoresis of the PCR products of desert locustsindicated a 710bp amplified DNA fragment on 1.5% agarose gel. DNA sequence analysis of PCR product indicated that specimens shared a 99-100% identity with Schistocerca gregaria gre...
Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*
...n method, the effect of liquid egg yolk and egg yolk powder preparations) on the activity of IgY antibodies was investigated. The results showed that IgY was stable below 70 oC, and the activity was significantly lost when it exceeded 70 °C; IgY was relatively stable in the range of pH 4-10, and the activity was significantly lost beyond this range; IgY had certain resistance to pepsin and trypsin. Among all four purification methods, the IgY an...
Aribah Naz1*, Uzma Badar1,Afsheen Arif2*, Zaid Qureshi1, Sondas Saeed1,Erum Shoeb1, Maqsood Ali Ansari1 and Obaid Yusuf Khan1
... micro flora possesses unique features that help them to survive under diverse environmental conditions including high salinity and low pH. Ease of detection is one of the prominent features of these glowing organisms to be the center of attention of many researchers and scientists. The focus of the study is to characterize and identify local Gram negative luminescent bacterial strain isolated from gut of Nemipterus japonicus commonly known as Japanese ...
Huma Abbas1*, Nazir Javed1,Muhammad Kamran2, Sajid Aleem Khan1Hira Abbas3, Ehetisham-ul-Haq2, Abdul Jabbar1, Mehwish Naz1 and Ihsan Ullah4
...modified split root technique. Efficacy of different application methods; soil drench and root dip was also tested. Results revealed that all the chemicals have more or less systemic effect against M. incognita. Cartap was found to be more effective in reduction of egg masses followed by Virtako in both treated and untreated root portion. Number of egg masses was reduced significantly in Cure and Azadirachtin in treated (45.7, 58.2) as well untreated ro...

Shahzad Malik* and Ayesha Khan

Gap Analysis of Farmers’ Capacity Building by Public and Private Agriculture Extension Sectors in Central Plain Valley of Khyber Pakhtunkhwa, Pakistan
...3%), and harvesting techniques (57.4%). There was highly significant (p≤0.01) difference among public and private services regarding capacity building of farmers in terms of farm management, improve marketing skills, harvesting techniques, organic farming, integrated pest management, seed rate ratio and orchard layout. Overall, there was a big gap in the performance of the public sector extension and private sector extens...

Khalil Ahmed1*, Amar Iqbal Saqib1, Abdul Rasul Naseem1, Ghulam Qadir1, Muhammad Qaisar Nawaz1, Muhammad Khalid2, Imtiaz Ahmad Warraich3 and Muhammad Arif4

Use of Hyacinth Compost in Salt-Affected Soils
...d soil is a simpler technique. Therefore, present research work was carried out to appraise the efficacy of water hyacinth compost as an ameliorant for improving the deteriorated properties of saline-sodic soil. Treatments included were; T1, control, T2, gypsum @ 100 % GR, T3, gypsum @ 50 % of GR, T4, hyacinth compost @ 15 t ha-1, T5, gypsum @ 50 % of GR + hyacinth compost @ 5 t ha-1, T6, gypsum @ 50 % of GR + hyacinth compost @ 10 t ha-1, T7, gypsum @ 50 % of...

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
...pproach is a useful technique for the identification of wilt complex pathogens. The study conducted to identify the polygenetic association of the pathogens of chickpea wilt complex. Universal Rice Primers based diversity analysis indicates high polymorphism within the collected wilt isolates. Primers ITS 1 and ITS 4 generated internal transcribed spacer fragments that were restricted four restriction enzymes (HhaeI, MboI, BsuRI, HinfI). PCR ITS-RFLP banding p...
Shahid Ali*, Aijaz Ali Channa, Waqar Ahmad, Rafia Akram and Imbasat Yousaf
...on, 60-70 L of amniotic liquid was expelled out and dam delivered completely developed alive male calf with a gross anomaly (presence of a hole in the skull) which could not survive and died soon after the delivery. After the completion of obstetrical case using obstetrical mutation and forced extraction, dam developed the signs of peritonitis, subcutaneous edema and labored breathing.
...
Murtaza, Shahid Ali*, Syed Attaullah Shah, Mehran Ahmad, Irfan Ullah and Jahangir Khan
Adoption of Hybrid Maize Technology in District Mardan of Khyber Pakhtunkhwa, Pakistan
...age random sampling technique. The logit model was used to measure the probability of adoption of hybrid maize technology. The estimated results of the model revealed that younger farmers were comparatively more adoptive of the hybrid maize technology. Education level, number of adult males involved in agriculture and land holdings of the farmers affected the adoption of the hybrid maize technology positively. The owners as compared to tenants and farmers havi...
Huma Abbas1, Muhammad Azhar Iqbal2, Muhammad Kamran3, Muhammad Umar Shahbaz3*, Haseeb Ullah Kamber1, Nazir Javed1, Muhammad Junaid1, Hira Abbas4 and Muhammad Ehetisham ul Haq3
Evaluation of Advanced Mung Bean Germplasm against Cercospora Leaf Spot and its In-vitro Management by Different Fungicides
...using poisoned food technique. Score at 100 ppm was found most effective (93.03%) against the disease as compared with other fungicides. From present research, it is devised that 16051 line may be used for future breeding program and Score at 100 ppm may be evaluated for field trials in future studies.

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Yasir Arafat1*, Syeda Nabahat2, Hafiz Ullah3, Afaq Ali Muluk4 and Azra5

An Analysis of Energy Supply and Oil Price Shocks on Agricultural Productivity of Pakistan
...g modern scientific techniques of testing and processing agriculture land and seeds for higher per acre yield which affects farmers directly. Most of the farmers either use electricity or oil to run the tube well for irrigation, increase in the prices of both electricity and oil affect crop productivity negatively. Consequently, government should provide electricity and oil in subsidized rates to the farmers at the time of cultivation and irrigation.

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Tahseen Ullah* and Noor ul Amin

In Vitro Antibacterial Activity of Medicinal Plant Extracts
...sing disc diffusion technique using Nutrient Agar Media for the growth of B. subtilis and E. coli bacteria. The ANOVA for inhibitions zones indicates the effect of plants, bacteria strains, and concentration on growth of bacteria in the bacteria growth culture medium and their interactions. The plants crude extracts were having significant effects on the bacterial growth in bacteria growth medium while bacterial strains and concentrations have also significant...

Shahzad Khan*, Munir Khan, Inayatullah Jan, Mahfooz Khan and Fida Muhammad Khan

Determinants of Sugarcane Yield in District Charsadda, Pakistan
...ortional allocation technique in the selected villages. For data analysis, profit margin, gross margin and Cobb-Douglas production function were applied. The study found that the per acre profit of sugarcane was 289.82 US$. Labour and fertilizers costs were found major components in the total variable cost. The profit margin was obtained as 37.37%. Empirical results of regression model found seed, farm yard manure and fertilizer positive and significant determ...

Shaiza Rasool1, Iftikhar Ahmad1*, Khurram Ziaf1, Irfan Afzal2, Muhammad A.S. Khan1, Muhammad Aashir Sajjad1 and Muhammad Zain Ali1

Seed Conditioning Effects on Germination Performance, Seedling Vigor and Flower Production of Zinnia elegans
...s seed conditioning techniques were assessed for uniform germination, seedling vigor, and flower production in zinnia (Zinnia elegans Jacq.). Two initial laboratory experiments evaluated the timing and frequency of seed magnetic stimulation to select the most promising treatments for field production testing and flowering. In the first experiment, a 3% moringa leaf extract (MLE) showed the earliest (2.8 d) and the highest germination rate (90%) with an average...
Sajad Ali1*, Naeem Ur Rehman Khatak1, Iftikhar Ahmad2, Jangraiz Khan3 and Azra4
Impact of Socioeconomic Factors and farm Size on Wheat Productivity: A Case Study of District Peshawar, Pakistan
...er to bring advance techniques or improve theirconventional agriculture practices to increase wheat productivity.

...

Khan Sher1*, Muhammad Subhan1, Muhammad Nisar2, Ali Hazrat2, Zahid Fazal1, Gul Rahim2, Imran Ahmad1, Riaz ul Haq1 and Shamia Bibi1

Genetic Diversity in Common Beans (Phaseolus vulgarus L.) Collected from Different Ecological Zones of Malakand Division (A Part of the Sino Japanese Region of Pakistan)
...s with some additional unique characteristics, such as early cooking, high productivity and protein significance as compared to other parts of the world, which could be utilized for evolving better quality and high yielding cultivars of P. vulgarus.

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Zafar Ahmad Handoo1*, Mihail Radu Kantor1 and Ekramullah Khan2

Description of Seven New Species and One New Record of Plant-Parasitic Nematodes (Nematoda: Tylenchida) Associated with Economically Important Crops of Kashmir Valley, Jammu and Kashmir (Part-1 of the series)
...red: Helicotylenchus siddiqii sp. nov. from soil around roots of Glycine max L. Miller, from Kashmir University Campus, Hazratbal, Srinagar, Kashmir; H. fotedariensis sp. nov. from soil around roots of Brassica oleraceae var. botrytis L. from Zadibal, Srinagar, Kashmir; H. harwaniensis sp. nov. from soil around roots of Lycopersicum esculentum Miller, in Harwan, Srinagar, Kashmir; H. mushtaqi sp. nov. from soil around roots of Brassica oleracea var. capitata L...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
..., plunged and stored in liquid nitrogen (-196°C). Semen straws were rewarmed at 37°C for 30 seconds and assessed for sperm motility, plasma membrane integrity (PMI), dead sperm percentage and the live sperm percentage. The data on the role of PVP on different parameters of semen quality were analyzed by using ANOVA and RCBD. Higher percentage (P< 0.05) of sperm motility (66.1±7.51 and 59.4±10.72) and PMI (72.9±5.39 and 75.7&plus...

Ghulam Akbar1*, Ali Ahmad2, Neha Arooj1, Muhammad Anjum Zia1, Aamna Rafique1, Sania Riasat1, Mohsin Raza1, Mahpara Qamar1, Shahneela Nusrat1 and Shakila Hanif1

Critical Update for the Treatment of Anemia by using Advanced Genome Editing Crispr Cas Technology
...age of gene editing technique for different types of anemia. In evaluation we defined currently developed genome editing device update used for the remedy of anemia, its mechanism of movement and sickle cellular mutation corrections.

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Rubina Munir1,2*, Muhammad Zia-ur-Rehman3, Muhammad Makshoof Athar1, Noman Javid1,4, Ayesha Roohi2 and Syeda Fatima Razavi2
...nfirmed by spectral techniques including FT-IR, 1H-NMR, 13CNMR, and mass spectroscopies. The purity of compounds has been established by elemental analysis. Free-radical scavenging activity of these compounds has been carried out by DPPH assay that showed these compounds to be effective radical scavengers. The compounds 7a, 7b, 7d, 7f and 7h have exhibited radical scavenging activity better than the reference compound BHT.
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Muhammad Umer Khan1,2*, Muhammad Farooq Sabar1, Atif Amin Baig3, Arif-un-Nisa Naqvi4 and Muhammad Usman Ghani1
...e globe and provides a unique maternal ancestry portrait of a person’s genetic pin code. Overall knowledge of mtDNA profiles of worldwide populations can benefit population genetics and forensic sciences. Consequently, this study was designed to establish the mtDNA profiles of the Shin ethnic group in Gilgit-Baltistan, the northern most territory of Pakistan. Phlebotomy was performed for a total of 79 maternally unrelated Shin volunteers. Genomic DNA was...

Asma Rashid Tariq1,2*, Saadia R. Tariq2, Tariq Mahmud1 and Misbah Sultan1*

Selective Capture of CO2 from Mixture of Different Gases using Cellulose Acetate, Polyimide, Polysulfone and some other Polymers based Mixed Matrix Membranes: A Review
...e days. A number of techniques are used for separation of CO2, methane and other hydrocarbons but mixed matrix membrane based separation is gaining significant importance day by day. A number of different polymers have been employed for the fabrication of mixed matrix membranes for example cellulose acetate, polyimides, polysulfone, pebax, polybenzimidazole, polymer of intrinsic porosity, polyurethane, chitosan and many more. Likewise, along with other fillers...

Muhammad Nazar1, Chaudhary Muhammad Ayyub2, Mujahid Ali3*, Malik Abdul Rehman4, Shoib ur Rehman5 and Saqib Ayyub2

Amelioration of Thermo-Tolerance Ability in Spinacia oleracea L. Cultivars by Foliar Application of Chitosan
...eas. There are many techniques to cope with these stresses but foliar application of chitosan was a very significant tool. Three cultivars of spinach (All Pure Green, Desi Local, and Lahori Palak) were grown at normal temperature 18/20°C (day/night temperature) and then after four weeks after emergence 40/32°C (day/night temperature) was given by gradually enhancing 2°C per day. After one week of heat stress (40/32°C), four treatments (25, 50, ...

Imtiaz Hussain1, Azhar Mahmood Aulakh2, Muhammad Sohail1*, Khalid Hussain2, Ansaar Ahmed3, Abdul Hamid3 and Muhammad Imtiaz3

Impact of Zero Tillage on Productivity of Traditional Mung Bean-Wheat Cropping System of Punjab, Pakistan
...ro tillage planting technique reduced total cost of production by Rs. 6236 ha-1 and helped farmer to earn additional Rs. 25601 ha-1 in terms of annual net returns than prevailing conventional tillage system. The benefit-cost ratio under zero tillage was calculated as 2.19 whereas 1.84 under traditional tillage practice. The results confirmed that new initiative (zero tillage) proved to be economical and resource conserving compare to conventional tillage.
Zulekha Zameer, Samreen Mohsin, Ammarah Hasnain, Asma Maqbool* and Kauser Abdulla Malik
Influence of Explant Sources on in vitro Callogenesis and Regeneration in Maize (Zea mays L.)
... case of split seed technique, the efficiency for callogenesis was 82% with the regeneration efficiency of 65.46%. For mature embryos the efficiency for callogenesis was 61% while for regeneration it was 78.06%. The results revealed that split seed technique is a preferable choice for explants for callogenesis, while mature embryos exhibit optimum frequency for regeneration in maize (Pioner 3025).

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Muhammad Yaseen1*, Muhammad Luqman1*, Zahoor Hussain2, Usman Saleem3, Asif Nawaz1, Tahir Munir Butt4 and Muhammad Umer Mehmood1

Assessment of Knowledge Level and Information Sources of Vegetable Growers regarding Tunnel Farming in District Sargodha
...e snowball sampling technique led us to have 8 off-season vegetable growers in Sargodha tehsil, 13 in Bhulwal, 27 in Bhera, 17 in Kot Momin, 9 in Shah pur, 11 in Sillanwali, and 5 in Sahiwal. This way a sample of 90 respondents was interviewed using a structured interview schedule. The content validity of the instrument was checked by the panel of experts and followed by the reliability test run through SPSS. Data were analyzed and evaluated by Microsoft Excel...
Neelam Pery1*, Fatima Ijaz1, Nayab Batool Rizvi1, Munawar Ali Munawar1 and Muhammad Imtiaz Shafiq2*
...ided drug designing techniques, we developed dual JAK3 and BTK inhibitors based on quinoxaline derivatives which show appreciable dual inhibition in enzyme assays. To our knowledge, this is the first-ever report on dual JAK3/BTK inhibitors.
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Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3

...ogenic cycle. Major techniques applied were; radioimmunoassay, light microscopy and stereology. Histological study of spermatogenesis was done at stage VII of spermatogenic cycle. After the treatment, in all groups, no alterations were observed in the levels of plasma FSH. The concentrations of testosterone and LH were found to be significantly reduced in kisspeptin treated groups of 1µg dose (p<0.01) and 1ηg dose (p<0.05) whereas the dose of 1...
Mushtaq Ahamd Khan1, Ziaul Islam2, *, Amin Ullah Jan3, Kamran Khan2 and Abdullah Shah3
...uno-chromatographic technique using strips (CTK, USA) and Indirect Enzyme Linked Immunossorbant Assay (i-ELISA). The study revealed that overall 57.28% sero-prevalence was recorded in women of child bearing age. Highest (56.46%) seroprevalence was recorded in pregnant women as compared to non-pregnant women (43.53%). Highest (57.3%) sero- prevalence was recorded in women having 21-30 years age. Notably, the highest (25%) prevalence was reported in second trime...

 Amna Arshad Bajwa1, Saher Islam1, Muhmmad Imran1, Karman Ashraf2, Arman Khan1, Muhammad Farhan Khan3, Imran Rashid2, Muhammad Yasir Zahoor1Waseem Ahmad Khan4 and Wasim Shehzad1*

... a simple molecular technique, that is precise with non-invasive sampling approaches such as faeces, would be valuable. In the present study a set of molecular markers was developed exploiting the AMLx/y gene to assess gender of Punjab urial population in Kala Bagh, using faecal samples as the DNA source. In our study, among 92 urial samples, 54 (58.69%) were identified as female samples, 34 (36.95%) were recognized as males while for remaining 4 (4.34%) sampl...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...ods, an alternative technique of propagation using in vitro methods is needed. The micropropagation method with the RITA® (Recipient for Automated Temporary Immersion System) is used in the production of a large amount of stevia biomass in approximately a short period. The forming of flowers is one of the limiting factors interfering with the metabolite production, as the content of steviol glycoside will decrease after plant flowering dramatically. This m...

Sajid Khan* and Shahnaz Akhtar

Effect of Farm Labor Transformation on Households Income in Central Districts of Khyber Pakhtunkhwa, Pakistan
...ugh random sampling techniques. The data were collected from 393 sample households and were analyzed by using paired sample t-test through Statistical Packages for Social Sciences (SPSS) and descriptive statistics, percentages, and rank orders. Farm labor transformation was found significantly affecting the earnings of households’ in the study area. Data revealed that monthly income from farm production and livestock increased significantly. Moreover, ho...
Inam Ur Rahman1, Syed Faizan Iftikhar2, Sanam Wagma Khattak3*, Wajahat Ali1, Azra4 and Sajad Ali1
South Asian Experience of Trade Liberalization: Implications for Food Security
...m effect estimation techniques. The trade liberalization link has been explored separately with each dimension of the food security. The empirical results show that the trade liberalization have negatively affect the food availability, accessibility and stability in the case of South Asia, while the food utilization is found to be positively associated with trade liberalization.

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Syed Muhammad1*, Badar Naseem Siddiqui2, Farhat Ullah Khan1 and Nawab Khan3

Self-perceived Constraints of Extension Field Staff Affecting their Working Efficiency in Khyber Pakhtunkhwa, Pakistan
...ew applications and techniques of modern technology for the purpose to increase agricultural production. The efficiency of EFS is, therefore, reflected by the farmers’ adoption level of modern recommended technology. The current study was designed and conducted during February 2018 for the purpose to find out self-perceived main constraints, affecting the efficiency of EFS in the province of Khyber Pakhtunkhwa, Pakistan. Five districts out of 26 were ran...

Asifa Kamal1*, Ghazala Noreen1 and Muhammad Amin2

Trends and Determinants of Fertility in Pakistan: Multivariate Decomposition Analysis (PDHS 1990-91 to PDHS 2012-13)
...ooled cross section techniques are used to analyse the data. It is concluded from multivariate pooled analysis of three surveys that age of women at first birth, region (Balochistan), wealth index (rich), education of both spouses and survey periods (2006-07, 2012-13) has significantly negative effect on the total children ever born to women in Pakistan. Child mortality (yes), husband’s desire for children (more than wife), ideal number of children, mari...

Muhammad Iqbal1, Muhammad Ehetisham ul Haq1*, Muhammad Kamran1, Muhammad Idrees1, Shahid Nazir2, Ihsan Ullah3, Sumera Naz1, Shaukat Ali1 and Muhammad Zafar Iqbal2

Morpho-Molecular Characterization of Xanthomonas Axonopodis Pv. Citri Associated with Kinnow (Mandarin) and its Management
...ng disc sensitivity technique. Streptomycin sulphate was found most effective to inhibit the colony growth of the bacterium. Streptomycin sulphate and Oxytetracycline were evaluated against the disease in field conditions. Streptomycin sulphate was found relatively most effective as compared to the Oxytetracycline.

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 Shafii Abdullahi Mohamed1, Abdiaziz Idiris Mohamud2*, Yonis Abukar Mohamed3, Pravin Mishra4 and Osman Sheikh Ali Jama5

Assessment of Knowledge, Attitude, and Practices of Population Towards Brucellosis in Benadir Region, Somalia
...ograms or any other technique that can be helpful.

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Muhammad Luqman1*, Roshan Hussain1, Muhammad Yaseen1, Muhammad Umer Mehmood1, Ijaz Asghar2 and Usman Saleem1

Comparative Analysis of Dietary Intake Patterns of Rural and Urban Communities of Southern Punjab, Pakistan
... equal distribution technique and interviewed by using structured interview schedule. Data were analyzed using Statistical Package for Social Sciences and the following key results were obtained. Most of the respondents from the study were above the 30 years of age (mature), illiteracy was high in rural area and most of the respondents were dependent upon business and farming as their livelihood source. Among rural communities, food group of fats and sugars wa...

Muhammad Kashif Khan1*, Muhammad Wasim Jan Khan2, Kaleem Ullah Jan Khan3, Muhammad Ishaq Khan4, Muhammad Faheem Jan Khan5 and Abdul Salam Lodhi6

 

Impacts of Construction Risk Attributes on Organizational Performance of Registered Construction Companies in Khyber Pakhtunkhwa, Pakistan
...various methods and techniques. The purpose of this study is to address the need of risk management practices in the construction industry of Khyber Pakhtunkhwa (KPK), Pakistan. The research work carried out with the involvement of 150 Pakistan Engineering Council (PEC) registered construction companies not only emphasizes on the crucial risk factors associated with construction projects but also insights into the impacts of the risk factors on the organizatio...

Mehreen Ijaz

Effect of Weave Type on Tensile Strength of Cotton Fabrics
...ype of construction technique for various functional purposes. Because it plays an important role in determining the performance behavior of finished fabric. Weaving is one of the commonly used technique and different types of weaves have different effects on mechanical and physical properties of fabrics. The study was experimental in nature. It was aimed at investigating the effect of weave type on tensile strength of cotto...

Salim Raza1*, Khan Gul Jadoon2, Sajjad Hussain1, Zahid ur Rehman1, Saira Sherin1 and Noor Muhammad1

Mitigation Plan for Identified Problems Faced by the Marble Industry in Khyber Pakhtunkhwa
... by applying modern techniques. Pakistan also hosts huge reserves of different qualities of marble but this sector could not developed to the international standards due to a number of difficulties. As a result of these problems, the raw marble excavated from quarries carries less economic value and could not compete in the international market. Marble industry of Khyber Pakhtunkhwa is selected for this study because major activities in this sector are being c...

Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu* 

... mediators, qRT-PCR technique. Quercetin significantly (P<0.001) reduced IgE levels in the asthma mice serum. Inflammatory cells in the mice BALF are significantly reduced in a dose-dependent manner through quercetin. Quercetin (P<0.05) significantly reduced the level of TNF-α, IL-1β, IL-4 and IL-5 cytokines and increased the level of IFN-π in allergic asthma mice caused by OVA. In contrast, there was no significant effect in the body weigh...

Sanaullah1*, Abdul Basit2 and Inayat Ullah3

Challenges and Prospects of Farm Mechanization in Pakistan: A Case Study of Rural Farmers in District Peshawar Khyber Pakhtunkhwa
...ulti stage sampling technique to gather data on the socio-economic features of the farmers and available machinery. A total of 240 rural farmers were randomly selected from two local union councils of provincial government Khyber Pakhtunkhwa Peshawar. The accumulated data were analysed using mean and standard deviation with an acceptance mean value of ≥3.00 and estimating logit model. Socio demographic features revealed that majority (52%) were in middle ag...
Arnab Tanveer, Shabana Naz*, Azhar Rafique and Asma Ashraf
...nd fecal floatation technique was used for isolation and identification of endo-parasites. Modified McMaster technique was used to calculate Eggs per gram. The data was subjected to ANOVA and Tukey-HSD (post hoc test). Six species of endo-parasites were identified from Jallo Wildlife Park. Eimeria (7500) and Ascaris (1100) were the most abundant. Murree Wildlife park had only two species i.e. Eimeria (38...
Muhammad Shafiq1,2,*,Rajwali Khan1, Ilyas Ali3, Sadeeq Ur Rahman4, Saif Ullah3Shah Jan Mohammad2, Mohammad Jan2 and Jinhu Huang2
...phite precipitation technique was used while IgG in colostrum were determined using digital Brix refractometer (Atago RX-1000) and serum total protein (STP) in cows and calves were analyzed by chemistry analyzer (Procan PS-520). Overall Achai breed of cattle showed highest mean value for pre and postpartum serum immunoglobulin (11.78 ± 0.92 mg/ml, 10.00 ± 1.09 mg/ml) than Jersey breed (9.80 ± 1.30 mg/ml, 7.86 ± 1.01 mg/ml) and HF br...

Shawaiz Iqbal1*, Nadeem Iqbal2, Usama Bin Khalid1, Muhammad Usman Saleem1, Adila Iram1, Muhammad Rizwan1, Muhammad Sabar1 and Tahir Hussain Awan1

Growth, Yield and Economic Analysis of Dry-Seeded Basmati Rice
...g crop establishing technique, which enables farmers to harvest economical rice production. Field study was conducted at Rice Research Institute, Kala Shah Kaku, Pakistan in 2014 and in 2015 to evaluate the different planting methods. Three dry-seeded rice (DSR) planting methods viz. (i) DSR-broadcast, (ii) DSR-drill, (iii) DSR-ridges were compared with the conventional transplanting (TR) method in lines having row to row and plant to plant distance of 22.5cm ...
Abid Mahmood Alvi1*, Naeem Iqbal1,2, Javid Iqbal3, Kazam Ali4, Muhammad Shahid1, Waqar Jaleel5,6,7, Hafiz Azhar Ali Khan8 and Tiyyabah Khan8
...o row spacing without mapiquate chloride (growth inhibitor), 30 cm row to row spacing without mapiquate chloride, and 30 cm row to row spacing with mapiquate chloride, using two cotton varieties (BS-15 and BS-70). The results indicated that the population of both sucking pests was more on variety BS-70 as compared to BS-15 cotton variety. Thrips and whitefly populations varied significantl...
Wentao Wang1,2, Xu Lin3, Jianshu Zhuo3, Dongjie Zhang2, Xiuqin Yang3* and Di Liu1, 2*
...g molecular biology technique for the first time. The CDS of the canonical transcripts (named V1) of porcine E2F3b is 1023 bp in length, and showed 93.35% and 90.62% identities with the homologues from human and mouse, respectively. The splicing variants were produced by exon skipping, alternative 5’ and 3’ splice sites alone or in combination. Minigene analysis showed that the splicing of porcine E2F3b is complicated. E2F3b isoforms are expressed ...
Maria Syafiqah Ghazali1, Azlan Che’ Amat2 and Nor Azlina Abdul Aziz1*
...ether sedimentation technique. All large felines in the zoo were infected with gastrointestinal parasites. A total of six species of gastrointestinal parasites were recovered including four nematodes (Toxocara cati, Ancylostoma spp., Toxascaris leonina, and Oxyuris sp.), a cestode (Spirometra sp.), and a protozoan (Isospora sp.). Half (n=5/10) of the large felines had mixed infections with Toxocara cati and A...
Oluwatobi Emmanuel Olaniyi1,2*, Chukwudiemeka Onwuka Martins2, Mohamed Zakaria2
...ampling point count technique using stratified random design was employed to survey (from November 2016 to December 2018) and choose 57 and 54 point stations around 14 and 24 lakes of PIW and PW respectively. Significant differences (p<0.05) existed in the encounter rate and effective detection radius of PPI and AP between PIW and PW. Both wetlands had low site occupancy, an unevenly distributed and non-significantly relative abundance (p>0.05) of PPI an...
Sana Mehmood1, Hafiz Muhammad Tahir1*, Muhammad Summer1, Sher Muhammad Sherawat2, Shaukat Ali1*, Sajida Naseem3
... of novel molecular technique of DNA barcoding to authenticate the morphological identification as compared to the conventional taxonomy. Moreover, the barcodes of the spiders has generated the reference molecular library which would help in establishing the phylogenetic relationships among various spider species for diversity analysis in future. Authentic molecular evaluation will also help to correct the misidentified specimens.
...

Shishir Sharma* and Laxmi Prasad Joshi

Current Insights on Stemphylium Blight of Lentil with its Management Strategies
...entally sustainable technique. Integrated disease management approaches along with Omics and weather prediction tools may be crucial in avoiding severe SB outbreaks. A comprehensive review article discusses the morphological as well as molecular dimension of pathogens, their effects, pathogenicity, integrated disease management approaches, and future views.

...
Luka Anthony*, Olugbenga Omotayo Alabi, Elizabeth Samuel Ebukiba and Vandi Gamba
...ulti-stage sampling technique was adopted. Data were analyzed using Multinomial Logit Model, Gini-Coefficient, Double-Log Regression Model, (Cobb-Douglas) and Principal Component Analysis. The results of the Multinomial Logit regression model show that among the significant factors that influence the choice of rice market outlets were: sex of the household head (P<0.10), educational level (P<0.10), access to credit facilities (P<0.05), extension servi...
Alam Zeb* and Amanullah Jan
...daptation of modern techniques and management practices in oil seed crops can reduce water shortage risks with optimum yield in oil seed crops. Sesame is being considered the most tolerant crop for avoiding drought conditions with higher yield. Experiments on “response of sesame to sowing methods, nitrogen and sulphur levels” were carried out in Agronomy Research Farms, The University of Agriculture, Peshawar. During two consecutive Khari...

Muhammad Riaz Gondal1*, Sultan Ahmad Rizvi1, Aaqib Riaz2, Waqas Naseem1, Ghulam Muhammad1, Mazher Iqbal3, Humara Umer3 and Inam ul Haq3

Apposite Sowing Techniques to Optimize Productivity and Profitability of Berseem
... appropriate sowing technique for optimization of fodder and seed yield as well as to maximize the net return. The experiment was conducted using five sowing techniques viz., broadcast, different row sowing with spacing of 15 cm, 30 cm, 45 cm and 60 cm. Sowing was done during first week of October during each year using seed of new berseem cultivar “Punjab Berseem” at rate of 20 kg ha-1. All fodder parameters of ...

Inam Ul Haq1*, Humara Umar2, Naeem Akhtar3, Muhammad Azhar Iqbal2 and Muhammad Ijaz1

Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review
... horticulture. This technique is used for proliferation of olive (Olea europaea L.) tree which is globally cultivated for centuries and its value added products are consumed worldwide. The traditional propagation techniques of olive are uneconomical with wastage of time while micropropagation of olive may expedite the development of important characteristics like biotic and abiotic resistance with high yield and premium qual...

Hafiz Hammad Ahmad1, Mukkram Ali Tahir1*, Noor-us-Sabah1, Ghulam Sarwar1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1 and Aneela Riaz4

Growth and Yield Response of Sunflower to Organic Amendments in Aridisol
...nalysis of variance technique and significant of treatments was tested using LSD test at probability level of 5%. The maximum fresh biomass (95.50 g), dry biomass (25.73 g), plant height (110.90 cm), head size (21.36 cm), number of achene haed-1 (864) and achene yield plant-1 (32.16 g) of sunflower was observed with application of NK + Rock phosphate at 7 g pot-1 + Wheat straw at 28 g pot-1 (T6) in cultivar Hysun-33. While, lowest value of fresh biomass (72.73...

Muhammad Umair Hassan1, Muhammad Aamer1, Muhammad Nawaz2, Abdul Rehman3, Talha Aslam3, Ubaid Afzal4, Bilal Ahmad Shahzad3, Muhammad Ahsin Ayub5, Faryal Ahmed1, Ma Qiaoying1, Su Qitao1 and Huang Guoqin1*

Agronomic Bio-Fortification of Wheat to Combat Zinc Deficiency in Developing Countries
...levels. The genetic techniques are costly, and sustainable, however, they are long term and requires large breeding activities and resources. Conversely, agronomic techniques appear to be short term, quick and economic solution to increase the Zn contents to meet human needs. The application of Zn as foliar sprays, seed priming, soil application and soil+foliar application effectively enhanced Zn uptake and grain Zn contents...
Muhammad Farooq1*, M. Javed Iqbal2, Rizwan Shukat2, Qayyum Shehzad3, Shabir Ahmed4, Elham Azadfar5, Abdul Saboor6, Ibrar Ahmad7, Iftikhar Ahmed Solangi1, Kaleem Kakar8, Shoaib Khan9 and Wang Yunyang1
Development of Onion Powder by using Egg Albumin as a Foaming Agent by Foam-Mat Drying Process
... by foam mat drying technique in which onion paste was treated with different concentration of egg albumin (0%, 4%, 8% and 12%) as foaming agent and Carboxyl methylcellulose (0.5%) as foam stabilizer and these were dried in hot air tray drier at different temperatures (55°C, 65°C and 75°C) with 3mm sheet thickness of onion foams. Effect of different concentration of foaming agent and drying temperature was studied on moisture and drying rate of oni...

Mansoor Qadir1*, Yousaf Khan2, Shahid Khan3, Djamaleddine Djeldjli3 and Muhanned Ismael Ibrahim Al Firas4

...iple access (OCDMA) techniques have attracted much attention by allowing each communicating subscriber’s simultaneous access of the entire medium with maximum security and spectral efficiency. This paper aims to design and analyze 2 dimensional OCDMA based coding scheme and corresponding network architecture to support high capacity Gigabit Passive Optical Network (GPON). The coding scheme is proposed by deploying diagonal eigenvalue unity (DEU) scheme a...
Nasir Rashid1,2*, Javaid Iqbal1,2, Umar Shahbaz Khan1,2, Mohsin Islam Tiwana1,2 and Amir Hamza1,2
...sing classification techniques. This research article mainly focuses on classification of motor movements recorded in the form of EEG signals of four limb movements. The database used in this study has already ben been utilized by the researchers earlier with different combinations of classification techniques based of feature extraction. The dataset has been recorded at Graz University of Technology, Austria and used for BC...
Tariq Mahmood Khalil1,2*, Muhammad Ajmal3, Iftikhar Zeb1,4 and Muhammad Azeem Khan1,5
 
 
... expected that this technique will also help in calibrating the existing salinity measuring devices and may minimize the influence of bias or prejudice induced due to instrumental errors.
...
Abdul Nabi Jatt1,2*
...Intriguingly, GC-MS technique showed the presence of five different AHL-signal molecules (e.g., C-6, C-8, C-10, C-12, and C-14) produced by C. freundii B1. Moreover, extracellular cellulase enzyme (exoglucanase and endoglucanase) activity was highly induced in addition to exo...
Muhammad Umar1, Mubashar Hussain1*, Muhammad Faheem Malik1, Muhammad Naeem Awan2 and David C. Lee3
...na, and no species was unique to a single site in the DVNP. Highest abundance, richness and diversity was recorded in Deva, with lowest community measures recorded in Barmala. Species richness and diversity peaked in September and was lowest in November. Applying a suite of community composition analyses, bird communities were significantly different across all the three sites (ANOSIM), with 10 species explaining 16.5% to 17.2% of these community dissimilariti...
Muhammad Mohsin Raza1*, Hera Gul2, Malik Muhammad Yousaf1, Sami Ullah3, Ghulam Sabir Hussain4, Mumtaz Hussain1, Jahangir Shah1, Bashir Ahmad1, Rao Wali Muhammad1, Ijaz Ahmad5 and Muhammad Zeshan6
 

Muhammad Zubair1, Habib Ahmad2*, Brian E. Hemphill3, Muhammad Tariq4 and Muzafar Shah5
...rent data reduction techniques. The consistency in phenetic affinities was even evident when they were considered in the backdrop of living ethnic groups of peninsular Indians and prehistoric inhabitants of the Indus Valley and South-Central Asia. When consideration was limited to the people of Northern Pakistan, the uniqueness of Khalil and Daudzai, was emphasized. A comparison of the people from Peshawar emphasizes the un<...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6 
... red, test tube and air liquid interface coverslip, (2) biofilm quantification at different time intervals and (3) monitor antibiofilm effect of three proteolytic enzymes including trypsin, chymotrypsin and proteinase k against S. Gallinarum. We observed that S. Gallinarum has strong biofilm forming ability as observed by dark black colonies on congo red medium. Quantification assays such as test tubes revealed significantly (p<0.001) strong b...
Qiang Tang1 and Qianli Tang2*
...ossible mechanism of isoliquiritin on wound healing in scalded rats. The results show that at 24 h after the last administration, wound healing rate of isoliquiritin group was higher than that of model group (p=0.024). Compared with control group, thickness of epidermis and dermis in model group was thinner (p<0.001), and width of subdermal collagen fibers also decreased (p<0.001), inflammatory fac...
Nosheen Basharat1, Usman Waheed2,3*, Muhammad Arshad1, Noor e Saba4, Iram Masood1, Akhlaaq Wazeer1, Ahmad Farooq1, Sadaf Moneeba1Abdul Rauf5 and Hasan Abbas Zaheer2,3

 

...on Medicine, Shaheed Zulfiqar Ali Medical University, Islamabad, from November 2016 to June 2017. Total 282 samples were selected after routine blood screening of Hepatitis B and C, of which 75 were HCV positive (asymptomatic), 75 were HBV positive (asymptomatic) and 132 were from healthy (without HCV and HBV) voluntary blood donors. Selected samples were tested for TTV presence by ELISA and PCR assays. TTV DNA was detected in 55.2% of the healthy donor’...
Tanveer Hussain, Masroor Ellahi Babar*, Akhtar Ali and Fiaz Hussain
...e camel proved to be a unique animal with physiological features. These attributes of the camel instigate us to explore its genetic architecture. Pakistan boasts a camel population of about 20 different breeds and a total of one million head. However, till date, scarce genetic data is available on Pakistani camel’s genomics. In efforts to explore the genetics of the camel, we chose to study the Inteferon beta 1 (IFNβ1) gene in in Mareecha camel and ...

Ahmad Ur Rahman Saljoqi, Muhammad Salim* and Iftikhar Ahmad 

 

...ifferent management techniques were studied against thrips (Thrips tabaci)nymph and adult populations in garlic crop during the year 2017-18. The experiment was laid out in Randomized Complete Block Design having two factors with three replications and six treatments. Treatments Emamectin 1.9 EC @ 750 ml ha-1, orange (Citrus aurantium L.) peel extract @ 5%, neem (Azadirachta indica) seed extract @ 5%, neem oil @ 3%, green lacewi...
Hafiz Hammad1, Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Abid Niaz4 and Muhammad Arif4
...nalysis of variance technique and significant of treatments was tested using LSD test at probability level of 5%. The maximum P accumulation (178.37 mg P pot-1), P uptake efficiency (1.90) and PE (phosphorus efficiency) ratio(747.15 g DM (g plant-1) of sunflower was observed with application of NK + Rock phosphate at 7 g/pot + Wheat straw at 28 g pot-1 (T6) in cultivar Hysun-33. While, the lowest value of P accumulat...
Ahmad Abrar Khan* and Muhammad Idrees
...introducing in farm techniques of intercropping, proper division and allocation of lands suitable to stone fruits, vegetables, grain crops, and other cash crops and relaxation in criteria for the government’s establishment orchard and non-government agriculture departments/agencies.
...
Tariq Ali Mastoi1, Zulfiqar Ali Mastoi2*, Zahoor Ahmed Khetran3, Ghulam Hussain Alizai2, Binish Baig4, Mitha Khan2 and Syed Jahangir Shah1
 
 
...ing random sampling techniques to investigate eligibility, education and obstacles of the respondents for obtaining micro finance loan from Zarai Taraqiati Bank Limited (ZTBL). Out of selected districts; ten respondents were chosen randomly who obtained micro finance loans. The information of the selected districts and respondents was obtained (ZTBL). Thus, the respondents were interviewed personally and necessary data were collected through structured questio...
Javaria Sherani1,2*, Muhammad Saleem Jilani1, Tanveer Ahmad2, Sohail Kamaran3, Abdul Manan2, Tehseen Ali Jilani2 and Mateen Sajid2
 
...ng is a widely used technique to improve yield, disease resistance and fruit quality. This work aims to assess the effect of grafting on production, fruit ripening, physical and chemical properties, and nutritional quality of the fruit. Rootstock has a great influence on plant growth and development, including fruit ripening. However, the presence of a cross-interaction between grafting and rootstock is often neglected. To explore cross-interaction between gra...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
...pe VII and contain the unique cleavage site motif 112RRQKRF117 with high relation to very virulent NDV Chinese strain Chicken /China/SDWF07/2011 strain with nucleotide identity percentage (99.3% -100%). The main causative agent of recent ND outbreaks in vaccinated broiler flocks in Menofia governorate, Egypt was found to belong to very virulent genotype VII. This strain was genetically identical to Egyptian genotype VII isolates isolated in the last period.
Ryan Septa Kurnia1* and Radiana Dhewayani Antarianto2
 

... tissue engineering techniques for reconstruction to mimic the microenvironment in respiratory tissue engineering.
...

 Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2

...ivided into five equal aliquots. The samples were diluted with a solution having different additives at 37 °C. The first two groups contained L-Cystine as an additive (2 and 4 mM), the next two groups had methionine as an additive (2 and 4 mM), and the last group had no additives (control). After dilution, the semen samples were cooled at 5 °C for 2 h and then stored in liquid nitrogen. For further evaluation, the sa...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2,  Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
... and, split into seven aliquots while extended with three different concentration (5, 7.5 and10 mmol) of L-cysteine diluents and followed by other three concentrations (1.2, 2.4 and 4.8 mmol) of vitamin E diluents and, the last aliquot of semen was extended with control (no additives) to semen diluent. The spermatozoa freeze-thawed quality were assessed and found that total motility, progressive motility, membrane functional...

 Amjad Ali1*, Pirzada Jamal Ahmad Siddiqui1, Naveed Ahmad2,Shabir Ali Amir3, Rafaqat Masroor3,Seema Shafique1 and Zaib-un-Nisa Burhan1

...rmined using visual techniques. Sea water physiochemical parameters were determined. A total of 58 fish species in 33 genera and 24 families were recorded at 10 different dive sites. High diversity occurred at Churna Island following Mubarak Village and Astola Island. Majority of the recorded fishes were planktivorous. Compare to Churna and Mubarak Village, physico-chemical parameters at Astola Island were found in limits as preferred by fish and coral communi...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

...d immune-peroxidase technique. Betacoronavirus 1 was detected by ELISA in 6 out of 20 fecal samples (30%), PCR detected 4 out of 6 ELISA positive samples at specific M.W. band of 236 bp by electrophoresis, and one sample was sequenced and submitted on Genbank with acc.no. MW173144, further phylogenetic sequence analysis revealed high percentage of identity with reference strains from different countries. Phylogenetic tree cleared that current research strain w...
Titin Herawati1,*, Indra Adiwiguna1, Ayi Yustiati1, Iis Rostini1, Roy Hendroko Setyobudi2, Hafizan Juahir3 and Asep Sahidin1
 

...and census sampling technique were applied. Research commenced from November 2017 to January 2018. Parameters measured are diversity index, dominance index, equitability index, and water quality. Data analysis employed comparative-descriptive method by mapping the species and comparing the diversity of fish living in both ends of Jatigede Dam. The results indicate that six families of fish are identified, consisting of 14 species. The fish diversity of Cimanuk...
Iftikhar Alam1, Atta Ulllah Jan1, Muhammad Ali2 and Muhammad Farooq3*

... three-round Delphi technique to identify and make consensus on opinions regarding priority research areas from experts in the field of nutrition. The initial questionnaire for ‘Round one’ consisted of single-open-ended, asking the experts to identify at least five most important nutrition research areas. Consequent upon repeated consensus building process, the experts identified and agreed upon ten most important nutrition research areas. The thre...
Saira Batool1, Safdar Ali Shirazi1 and Syed Amer Mahmood2*
 
 
...obtained from RUSLE technique. Tree plantation, check-dams and strip cropping can also reduce soil loss when implemented in critical areas highlighted in this research.
...

M. Javed Iqbal1, Rizwan Shukat1, Muhammad Farooq2*, Iftikhar Ahmed Solangi2, Naila Ilyas3, Rahman Ullah4, A. Shakoor5, Muhammad Bakhtiar6 and Faraz Ahmed7 

... by foam mat drying technique in which onion paste was treated with different concentration of soy protein (0%, 4%, 8% and 12%) as foaming agent and Carboxyl methylcellulose (0.5%) as foam stabilizer and these were dried in hot air tray drier at different temperatures (55°C, 65°C and 75°C) with 3mm sheet thickness of onion foams. Effect of different concentration of foaming agent and drying temperature was studied on moisture loss drying rate of on...

Salma Javed and Samreen Khan*

...mann’s funnel techniques. Specimens were killed, fixed and identified up to species level under light microscope. Afterward, hundred fresh handpicked nematodes were inoculated on both fully grown fungal species at 25ºC. The nematode attraction and mass culture were appeared on Fusarium oxysporum only. On the first week, propagation rate was noted approximately 2.5x10³ followed by 3.9x10³ on the second week and finely after three weeks incu...

Aamer Sattar1, Sadia Sultana1*, Abid Niaz1, Muhammad Aftab1, Ghulam Sarwar2, Irfan Rasheed3, Muhammad Shoaib4, Raheela Naz1, Amina Kalsom1, Nisa Mukhtar1, Farah Rasheed1, Arfan ul Haq1, Munazza Rafique5, Muhammad Arif1, Sarfraz Hussain1 and Jafar Salim6

Raza Ullah Khan1*, Ahmad Khan1, Mohammad Zameer Khan1, Fayyaz Hussain1, Zafar Islam2 and Muhammad Asad Hameed2

...characterized using techniques such as spectrophotometer, High Performance Liquid chromatography, Fourier transform infrared spectroscopy and atomic absorption spectrophotometer. The analysis showed the presence of hydrophobic and hydrophilic sites. The Fourier infrared spectroscopy analysis showed spectrum bends at different wavenumbers while elemental analysis show existence of different macro and micro nutrients which can...

Manzoor Hussain Memon1, Khalid Khan2, Anjum Parvez3, Sarfaraz Ahmed Shaikh4, Khan Mir Khan2, Guo Xiangyu5*, Mohammad Nasrullah6 and Saima Liaqat7

...multistage sampling technique. Building on quantitative industry figures the study also highlighted various qualitative problems and factors which are responsible for the production, and profitability of the poultry industry in the district. The results of the study revealed that the unfair and unwarranted distribution of profits among producers, commission agent and retailers are the major impediment to the extension of the poultry industry in the district. T...
Aiguo Zhou1,2, Di Sun1,2,Shulin Liu 1,2, Yongyong Feng1,2, Yue Zhang3
Yanfeng Chen4, Shaolin Xie1,2* and Jixing Zou1,2*

 

...uals, which were eight unique and one shared haplotypes. In addition, the genetic distance for inter-species of all the haplotypes ranged from 0.000 to 0.004. And the mean pair-wise genetic distance between the two color morphs were estimated to be 0.001. This indicated that the “Bicolor” and “White” types of northern snakehead belong to the same species at the molecular level. Moreover, all the haplotypes were gathered together by the ...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
... Gram staining, gelatin liquefaction test (GLT) and polymerase chain reaction (PCR), respectively. Out of total, 85 (31.3%) were positive for C. perfringens type D on PCR. The results revealed 6-34% prevalence of C. perfringens type D in different regions. Similarly, significantly (P˂0.001) higher prevalence was observed in lambs (54%) as compared to sheep (23%). For gross and histopathological...

Muhammad Yousif Jakhrai1, Ahmed Nawaz Tunio2, Ali Mujtaba Shah1*, Tarique Ahmed Khokhar1, Muhammad Mohsen Rahimoon1

...epidural anesthetic technique and high epidural anesthetic drugs injected to induce epidural analgesia in small ruminants like caprine and ovine. The information was obtained from published papers of last twenty years via research gateways such as; PubMed, Scopus and Google Scholar. Small ruminant anesthesia, local anesthesia in ruminants and high epidural analgesia in sheep and goats were key points for this review. However, these research papers were include...

Hafiz Basheer Ahmad1*, Sajida Habib2, Waqas Shafqat Chattha3, Rizwana Qamar2, Shazia Saeed2, Abdul Khaliq1 and Salsabeel Rauf2

Qaiser Shakeel1*, Rabia Tahir Bajwa1­, Yasir Iftikhar2, Mustansar Mubeen2, Muhammad Luqman3, Waqas Ashraf1 and Ifrah Rashid4

...rough poisoned food technique with triplicates. The crude extract of all the plants showed a better inhibitory effects on the pathogen’s (P. digitatum) mycelial growth rather than fungicides. Among all the plant extracts, the best result was showed by garlic extract with 87% of mycelial growth inhibition followed by moringa with 82%, the best concentration is 100mg/mL. Besides, Ridomil gold showed the most effective control against ginger soft rot with 8...
Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
... purposive sampling technique was applied to collect n=200 milk samples from different regions of goat populated areas of district Faisalabad-Pakistan. Using surf field mastitis test, collected milk samples were screened for subclinical mastitis at the spot for subsequent identification of pathogenic strains of S. aureus through microbiological examination in the laboratory. Non-probability statistical tools conferred 42% (84/200, CI=35.37-48.93) preval...

Allah Bakhsh1, Attiq Akhtar1*, Fiaz Hussain1 and Shabbir Ahmed2

...a canopy management technique that provides a cost-effective and high-quality grape yield. In order to boost grape quality and minimize disease incidence in the King’s Ruby variety, FZLR was used for two seasons in a row. Total leaf removal was used before bloom, during full bloom, and four weeks after bloom, and was compared to an untreated control (non-defoliated). In all leaf removal treatments, titratable acidity and bunch rot incidence decreased sig...
Suman Bhattarai1*, Subodh Raj Pandey2, Jaya Prakash Dutta3, Meghnath Timalsena4 and Rajendra Bam5
...ple random sampling technique. Interview using semi-structured questionnaire schedule was carried out for primary data. Cobb Douglas production function, multiple regression, and paired t-test were used for the analysis of collected data. Multiple regression model revealed that family labor (p=0.68), age(p=0.007), family size (p=0.001), experience (p=0.007), harvesting (p=0.000) and maintenance of flowering plants (p=0.057) have significant effect on honeybee ...

Mohammad Tariq Aziz* and Ayesha Khan

...ilization of modern techniques. Access to information in agriculture can be extended through ICTs which support and share knowledge. The research study was conducted to find out the role and utilization of information and communication technologies (ICTs) for availing agricultural information in the research area. District Charsadda was the universe of the study and Multi Stage Sampling technique was used to select responden...

Chala Hailu Hussen and Fikiru Temesgen Geleta

... two-stage sampling techniques, data was collected from 160 farm households of selected Western Shewa zone districts, namely Ejersa-Lafo, and Bako-Tibe. Data was emanated from primary sources, which administrated by semi-structured questionnaire using personnel interview. On top of this, the focus group discussion was carried out on particular topics to supplement primary data source discussions. Both descriptive statistics and a probit regression model was em...

Muhammad Waqas1*, Muhammad Shoaib2, Muhammad Saifullah1, Adila Naseem4, Sarfraz Hashim1, Farrukh Ehsan1, Irfan Ali3 and Alamgir Khan1

...lligence (AI) based techniques: TB (Tree Boost), DTF Decision Tree Forest, SDT Single Decision Tree and conventional Multilayer Perceptron Neural Networks (MLPNN) are used for predicting streamflow of Jhelum River basin. The dataset was divided into two sections, i.e., training dataset (1971-2000); and testing dataset (2001-12). The tendency investigation was done by the Sen’s slope and Mann–Kendall (MK). Decreasing trends annually and seasonally f...

Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*

... acetone extraction technique. Ponds water sample (50 liters) was filtered through plankton net of 20 µm pore size and preserved in 4% formaldehyde for the analysis of phytoplankton. Subsequently, the phytoplankton were counted under microscope. Result showed that water quality parameters of all fish farms were suitable for farming purpose excluding Shamimul Hassan Farm, Fish Farm Green Co. and Ghulam Ali Nizamani Farm, where high level of total hardness...

Mian Muhammad Arif* and Malik Muhammad Shafi

...ugh random sampling technique and data were collected for 483 flocks by means of interview schedule. Descriptive statistics was used for general analysis, while budgetary technique was used for profitability analysis. Ordinary Leased Square (OLS) technique was employed to find the effect of different inputs on profit of broiler poultry farms. The ANOVA tool was used to know the variations ...

Fazal Subhan1,2, Abdul Malik1, Zia Ul Haq1 and Tariq Mahmood Khalil1,3*

...rements, irrigation techniques need improvement to get maximum yield with reduced irrigation. A field experiment was performed at Mardan region, Khyber Pakhtunkhwa, Pakistan, during 2018-19 with the objectives to evaluate the effect of different furrow irrigation techniques and deficit irrigation on cauliflower yield and water use efficiency (WUE). Three furrow irrigation techniques; alter...

Kiran Shahjeer1*, Gauhar Rehman1, Khurshaid Khan1 and Toheed Iqbal2

...engthened by modern techniques of molecular identification.

...

Misbah Ali1, Safdar Ali1, Muhammad Ahmad Zeshan2*, Rana Binyamin3, Nadeem Ahmed4, Muhammad Usman Ghani5 and Awais Ahmed Khan2

.... The poisoned food technique was used for in vitro trials with three concentrations (150 ppm, 200 ppm, and 250 ppm). The most effective concentration of fungicides from laboratory experiment was used in field conditions against red leaf spot of sorghum. Results showed that Cabrio Top was the most effective fungicide by showing 88% fungal inhibition while Native also exhibited good results by showing 78.1% fungal inhibition. The remaining 2 fungicides Melody D...

Weenghar Ali Chandio1, Tajnees Pirzada1*, Abdul Majid2 and Farzana Rashid3 

... by using different techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR), and scanning electron microscopy (SEM). Finally, the effect of extracted humic acid on wheat seed germination profile including root/shoot length, germination and fresh/ dry weight was investigated by applying varied concentrations of HA (0, 5, 10, 15, and 20ppm).UV-Visible spectroscopy confirmed the presence of HA in soil sample. XRD demonstrated that HA extract...

Mehnaz Safdar1, Urooba Pervaiz1* and Dawood Jan2

...multistage sampling technique. Descriptive analysis revealed that 305 women farmers were involved in rearing poultry. A 5 point Likert scale was used, showed that women farmers had various constraints out of which Pardah, mobility and lack of information source were ranked on top with Likert score value of 4.12, 4.00 and 3.94, respectively. Due to male dominant society, 62.8% of the decisions were taken by spouse. Results of Chi-square analysis showed a signif...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
...scence quantitative technique to test ZFX, Bcl-2, and BAX expression in mice kidney tissue on 1 d, 7 d, 14 d, 21 d, 42 d, and 90 d after birth. The results showed that with the maturation of mouse kidney, the cell proliferation activity decreased, and the expression of ZFX, Bcl-2, and BAX were down regulated. The ZFX gene may participate in the developmental process of the kidney through the balance of cell proliferation and apoptosis regulation.
...

Ahmed A. Kheder

...olecular diagnostic techniques. Virus incidence was performed in different locations in five governorates during 2018-2020. Forty-one samples out of 693(5.94%) reacted positively to SLRSV. Specifically, the percentages of infection were 7.3, 6.7, 5.4, 5.16 and 2.63% in El-Qalyubia, El-Beheira, El- Menoufia, El-Sharkia and El-Ismailia governorates respectively. SLRSV was mechanically transmitted from infected strawberry plants onto ten herbaceous plant species,...

Muhammad Shahzad1*, Syed Amer Mahmood2, Athar Ashraf3, Amer Masood2 and Saira Batool4

...d on the DD and TSR techniques. These are the interrelated procedures, i.e. TSR investigation may be used to distinguish areas with low and higher DD values and their connection with the active tectonics. The three-dimensional maps for TSR and DD are generated using a moving window size of (1-4 km). TSR and DD maps show interesting results that correspond to the relative sensitivity of the landscape to regional neotectonics. DD exposed the neotectonic activity...

Iftikhar Jan* and Sahib Alam

...dium by using ELISA technique. The results showed that isolate AFS32 produced highest level of total aflatoxin (230.78 µg Kg-1) while isolates AFS5, AFS17, AFS25 and AFS33 produced no aflatoxin. These nonaflatoxigenic isolates were considered as isolates of interest and could be used as biocontrol agent in agricultural fields for prevention of pre-harvest aflatoxins production in different crops.

...

Shahzad Khan1*, Munir Khan1, Arif Alam2, Ikram Shah2, Mahfooz Khan1 and Fida Muhammad Khan1

...ortional allocation technique. However, primary data were randomly collected through face to face interview from the selected respondents with the help of a semi-structured questionnaire. For data analysis, profit margin, gross margin and Cobb-Douglas production function were applied. Therefore, the labour, tractor, and fertilizers were found major components in the total variable cost. The profit per acre was obtained as Rs.12714. The profit margin was obtain...

NawalM. Abdulla1, M. Haroun1*,Mohamed A. Shalaby2and Ahmed A. Elsanousi2

...d as a confirmatory technique, successfully amplified and
detected cDNA fragments corresponding to the NDV RNA extracted from amnioallantoic
fluids (AAF). Sequence analysis and phylogensis of the NDVQF13 strain using 150bp
primer sets revealed 112R-R-Q-K-R-F117 amino acid motif confirming velogenicity of the
strain and its relation to genotype group VII, respectively. NDVQF13 failed qualification
for ...
El-Dougdoug K.A.1, Sofy A.R.2, Rehab A. Dawoud3 and Korkar H.M.4
...on osmopreservation techniques. Osmotherapy wasapplied for
eliminationbanana bunchy top virus(BBTV) through preservation of shoot tips on
MS media containing high sucrose concentration at low temperature. Shoot tips and
subculture 1st of banana plantlets were preservedsuccessfully for 12 months on
storage MS medium supplemented with 40, 50, 60 and70 gL-1 at 10, 15 and 20ᴼC.
The highest survival perc...

D. N. Abd-elshafy1, 2 and M. M. Bahgat2, 3

... using cell culture techniques.

...

Amal Mahmoud1&2 and Medhat H. Hashem3

... alignment resulted in unique amino acid substitutions (V320→L and N464→T) and (I284→L, T300→I and K334→R) in pol and env genes respectively. Further prospective studies are needed to confirm the role of these mutations in the development of HCC.

...

Enas K. Abo-Elmagd,Kouka S. Abd El-Wahab,Azza H. El- Salakawy

...e tested by another technique like RT-PCR.

...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...utomated sequencing technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sharkia/2013/ FMD / (KJ210080) revealed (99–100%) identity with Egypt 2012/ and Palestinian-Gaza virus. Phylogenetic analysis of serotype O of this study from Ismailia (KJ210076) , Alexandria (KJ210074) and El-Mania (KJ210077) showed 100% identity with each other and 92% identities with Asian type O sequences from Pakistan, Iran and Turkey while FMD/A/3D/Egypt/Al-Fayoum /2013/K...

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

...luorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotavirus (15%) infection using ELISA test. Successful isolation of the rotavirus on MDBK cell line from two samples from the positive ELISA samples. The beginning of characteristic cytopathic effects (CPE) were observed from the 3rd...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...luorescent antibody technique (IFA) which was considered a rapid and accurate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven out of twenty one nodular samples were positive by (IFAT) in CAM, which prove that PCR and Real Time PCR are much sensitive and rapid diagnostic tool of L...

Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3

...luorescent antibody technique and isolation on tissue culture using BHK cells. Direct confirmation was performed by Real time RT- PCR and gene sequencing was performed directly from brain samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conducted. The % of nucleotide identities of the Egyptian recent isolate (Egy/cairo /2014) with three previous Egyptian isolates were 98.4-98 and 98.4...

El-Bagoury, G.F., El-Nahas, E.M., El-Habbaa, A.S.

... immunoflourescence techniques, and then molecularly identified using reverse transcription polymerase chain reaction (RT-PCR). Both cattle and buffalo isolates behaved the same pattern for serological and molecular identification in correlation with BEFV Webster reference strain and showed an amplicon size 473 bp for G2 gene. In conclusion, BEFV field strains from cattle and buffaloes seem like but further molecular characterization was required to study thei...

Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4

...ent assay (I-ELISA) technique. The produced antisera were evaluated and compared with foreign antisera using tissue printing immunoassay (TPIA) and dot blot immunoassay (DBIA). The produced antisera were found more efficiency in development the purplish blue color than the positive reaction when the foreign antisera applied, therefore it should be use produced antisera from Egyptian isolates specific for detection of sugar beet viral infections as a good repla...

Samah A. Mokbel, Ahmed K. El-Attar

...culture
technique and radiation treatment represented a short method for high level
management. To investigate the effect of gamma rays to produce healthy plantlets, the
hibiscus witches' broom phytoplasma was transmitted by grafting into healthy
hibiscus plants, the in vitro-infected hibiscus explants were exposed to gamma
radiation at different doses - 5 gray (Gy), 10Gy, 15Gy, 20Gy and 25 Gy- emitte...

Ahmed K. El-Attar; Samah A. Mokbel; Ali H. Hamed

...culture
techniques. To achieve our aim, the virus has been isolated from naturally infected onion
plants grown in five Egyptian Governorates, Gharbia, Qalyobia, Giza, Fayoum and Beni-
Suef then mechanically transmitted onto healthy onion plants in an insect proof
greenhouse. The virus identification was done by indirect ELISA using specific
antiserum and confirmed by Reverse Transcriptase-Polymerase C...

K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2

...On the other hand, the unique band appeared only in infected plants of Valor cv. with MW
85 KDa. Total phenols and proline were increased in tolerant and resistant potato cultivars
compared to susceptible cultivars and healthy ones. Scavenging enzymes (SOD, CAT, POX and
PPO) were increased mostly in tolerant and resistant cultivars compared with susceptible
cultivars and healthy ones. The total number of phenol o...

Mohamed I. Azzam1, Safaa M. Ezzat1, K. A. El-Dougdoug2, Badawi A. Othman2

... 6, 2, 9, 10, 8, 10, 7 unique fragments and HindIII
produced 2, 3, 0, 1, 2, 5, 4, 3 unique fragments while BamHI produced only 4, 0, 1, 1, 0,
4, 2, 1 unique fragments, for the eight phage isolates, respectively. Out of fifteen tested
sites, two only (El-Rahawy drain outlet and Sabal drain outlet) were found to be polluted
with enteroviruses w...

Rehab A. Dawood1; Entsar A. Nassar2; and K.A. El-Dougdoug 3

...sing tissue culture technique. Osmotherapy refers to culture meristem – tip and stem nodes at MS medium saturated with sucrose. Stem nodes of potato were successfully stored for up to 12 month at osmo-preservation medium supplemented with sucrose 50, 60 and 70 gl -1 at 210C. The survival percentage was recorded 85.1, 81.2 and 50.8 for 50, 60 and70 gl -1 respectively for 12 months at 210C. All survival plantlets revealed viability and resumed growth on fr...

Amel, S.M. Abo-Senna1; M.A. Nasr-Eldin2; B.A. Othman3 and A.A. Megahed4

...d as ZYMV by RT-PCR technique using high specific primers as 113 bp fragment within the nuclear inclusion protein b (NIb) coding region of the virus genome. The cytopathological effects of ZYMV on the infected cells were completely chloroplasts destruction, and deformation of vascular bundles. The effect of late ZYMV infection decreased fresh weight and yield of squash plants.

...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...er DNA manipulation techniques such as restriction digestion,
Southern blotting and cloning.
...
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1 
...ght blue. Different techniques for transmission of
phytoplasma to healthy tomatoes and periwinkles in an insect-proof greenhouse were
tested, including mechanical inoculation, wedge grafting, parasitic plant dodder (Cuscuta
campestris), insects in the family Cicadellidae (leafhopper, Empoasca decipiens) and in
germinated seeds within the fruit, suggesting mechanical inoculation and seedtransmissible
w...

Samah A. Mokbel, Soad H. Taha, Mahmoud M. Abd-El Hamid, Ali H. Hamed

... evaluated by ELISA technique. Results demonstrated that application of 1000 mg/L lactoferrin by spraying or combined with tissue culture proved to be an effective method for PVX-inhibition as compared with other concentrations. Also, results of antiviral activity of lactoferrin at concentration 1000 mg/L showed great potential as phytotherapeutic source to produce quality and health plantlets for rapid and large scale in vitro production.

...

Aly M. Abdel-Salam1 and Samah A. Mokbel2

...the electro-elution technique yielded nucleoprotein which
had Amax and Amin at 260 and 240 nm respectively. Electron microscopy examination
showed spherical virions with ca. 26 nm in diameter.
...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

... immunofluorescence technique in the brain sections of positively inoculated mice. Reverse transcriptase polymerases chain (RT-PCR) reaction was performed directly on buffy coat samples for molecular identification of the virus showed 470 bp clear band in gel electrophoresis. The sensitivity of the utilized techniques in the identification and diagnosis of bovine ephemeral fever disease revealed that the virus isolation is a...

Sahar A. Youssef1; Manal A. El-Shazly1,2; Azza G.Farag1,3; Eman A. Khattab1,2

...tests and molecular technique. The virus was identified serologically by direct ELISA, dot and tissue blotting immune-binding assay using authentic and induced antiserum for PNRSV.RT-PCR with specific PNRSV primer was used to confirm the obtained results. No amplified product was obtained from healthy control. The partial RNA3 movement protein product from PNRSV infected rose directly cloned using the TA cloning system. Nucleotide sequencing revealed the clone...

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and M. A. Shalaby2

..., 2002 using RT-PCR technique that based on the amplification of fragments of N-protein. All batches are also subjected to sterility tests for detection of bovine viral diarrhea virus as possible extraneous virus contaminant using RT-PCR and detection of mycoplasma as possible bacterial contaminant using PCR technique. All of the four batches were positive when tested using specific primers and they were free from BVDV or my...

Asfand Raheel1, Syed Zulfiqar Ali2, Muhammad Waris2, Muhammad Basharat2, Basheer Ahmed2, Muhammad Arshad Ullah3, Syed Ishtiaq Hyder4 and Taqi Raza4*

...with these advanced techniques. Keeping an eye on good agricultural practices (GAP) standards, new advanced approaches should be reflected as gear of a combined decay control strategy. Postharvest decay is totally dependent upon harvest and handling practices. Environmental conditions mainly affect the fruit infection and source of contamination under subtropical climate while in tropical climates contamination of horticultural products affecting postharvest d...

Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed

...luorescent antibody technique (IFAT) using specific hyper immune serum against LSDV. Further identifications were carried out by polymerase chain reaction (PCR) and clinco-pathological investigation. Results: The results showed that 11/30 biopsies were positive by AGPT, 19/30 by IFAT and 30/30 by PCR. While results of sero-diagnosis showed that 45/100 from apparently healthy non vaccinated cattle and 68/100 from vaccinated cattle were positive by SNT respectiv...

Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5

...luorescent antibody technique (IFAT) using specific hyper immune serum against Lumpy Skin Disease Virus. Further identifications were carried out by polymerase chain reaction (PCR). Results: 15 samples were +ve for LSDV by conventional PCR.The results showed that 11/23 biopsies were positive by IFAT. Molecular identification of LSD virus by using RT-PCR, revealed positive for amplification of bands at the predicted molecular size (1926bp). Neutralizing antibod...

Aly M. Abdel-Salam

...the electro elution technique devised by the author of the present study. Rabbit immunization was used for induction of antisera for CYSDV and CVYV. RT-PCR was used to amplify coat protein genes for the two viruses using specific primers. DAS-ELISA and immuno-blotting were used for evaluating the induced antisera. Results: Antisera for CYSDV and CVYV were produced efficiently and used for virus diagnosis through DAS-ELISA, DBIA, and TBIA. RT-PCR confirmed the ...

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

.... Egypt/Qal/014p had a unique amino acid substitution at residues 39 (Serine), 40 (Tyrosine), 41 (Lysine), 64 (Glutamate) and 69 (Valine) of the S1 proteins from H120 and M41.The cross-neutralization test revealed antigenic relatedness of Egypt/Qal/014p with Massachusetts serotypes. The commercial H120 vaccine conferred partial protection against proventriculitis induced by Egypt/Qal/014p. Conclusion: IBV strains exhibiting proventriculitis were found co-circu...

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and Mohammed A. Shalaby2

...e of some molecular techniques as conventional reverse transcriptase polymerase
chain reaction (RT-PCR) and real time RT-PCR in identity test of PPR vaccine.
Methods: Four batches of PPR vaccines that produced in Egypt by Veterinary Serum and Vaccines
Research Institute (VSVRI) were collected and tested for identification of PPRV by conventional RTPCR
using two primer sets targeting F-gene and N-gene and real tim...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
...sion: The molecular techniques based on the RPO30 and ORF 103 genes are efficient for the
characterization and differentiation of Capripox viruses. This finding may provide new information on
the epidemiology of SPV in El Menofiya governorate.
...

Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2

...>
the rRT-PCR technique from final product of these inactivated vaccines.
Results: The data of this study showed that sufficient HA antigen content and similar HA sequences
with the field HPAI challenge viruses are needed to produce a consistent and high protection percent,
but, they are not enough to assess the vaccine efficacy.
Conclusion: The invitro assessment of protection was not considered an alterna...
Wen-Feng Li, Rong-Yue Zhang, Jiong Yin, Hong-Li Shan, Xiao-Yan Cang, Zhi-Ming Luo, Xiao-Yan Wang, Jie Li and Ying-Kun Huang*
...ly friendly control techniques of Sesamia inferens Walker and Argyroploce schistaceana (Snellen). The use of new sex pheromone traps of sugarcane borers (6 a/ha) + 0.05% Abamectin•10 billion active gemma/g Bacillus thuringiensis WP (1800 g/ha) or new sex pheromone traps of sugarcane borers (6 a/ha) + 200g/L Tebufenozide SC (1500 mL/ha) were the best. The control effect of dead heart rate and bored stem rate could be more than 69.98% a...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...R is an appropriate technique for the detection of EHV-1 present in blood
of a latently, or silently infected horses. The application of this nested PCR is considered to be a rapid
diagnostic tool for use in veterinary laboratories. Thus it will enhance the detection and
characterization of the circulating EHV-1 in Egypt. Control strategy for EHV-1 viruses in vaccinated
and non-vaccinated animals needs to be addr...
Othman N. O. Mansour 1, Naglaa Hagag 2, Momtaz A. Shahein 1, Sayed S. Hassan 1 Ahmed
A. EL-Sanousi 3 and Mohamed A. Shalaby3
...sing indirect ELISA technique.
Results: From 1500 nasal swabs of imported camel breeds collected in Birqash veterinary quarantine,
ten samples (0.66%) were positive for MERS-CoV by real time RT-PCR. Out of 195 serum samples
which collected from imported camels in abattoirs of some governorates, 110 samples (56.4%) were
positive for the presence of specific antibodies against MERS-CoV while the nasal swabs of thes...
Allam A. Megahed, Badawi A. Othman, Samar S. El Masry, Abeer A. Faiesal and Mohamed A. Nasr Eldin
...pt using the RT-PCR technique.
...

Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2

...he laboratory-based techniques
such as (DAS)-ELISA, and RT-PCR using a pair of primers specific to the AMV-CP gene.
Phylogenetic analysis results indicated that AMV-Egypt that isolated from Basil is most closely
related (96.4%) to the AMV-Spain strain isolated from Hibiscus plants.
Conclusion: Pathological investigation may provide insights into the alterations of the cell after
viral infection and un...
Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...ato cv. SSB revealed 3 unique fragments
with 98-A ISSR primer, 3 unique fragments appeared in SSB cv. treated with 3mM NaN3 with 98-B
ISSR primer, and E-9 ISSR primer revealed 6 fragments in EMS (0.40%) treated CR cv. and 5
fragments in 3mM NaN3 treated SSB cv. Indicting changes in the genetic make-up of the treated
tomato plants. No similar fragments were observed in the contr...

Tahsin S. Shoala1, Ahmed K. El-Attar2, Ahmed I. Abd El Maksoud3

...r PLRV using RT-PCR technique, and all of the plants were PLRV positive. MS
media were prepared, autoclaved, and active natural materials (Glycyrrhizic acid ammonium
salt (GAS), Glycyrrhizic Acid (GA), Curcumin (CU) and Rosmarinic Acid (RA) were
sterilized and added to MS media at different concentrations.
Results: Potato plants those treated with Glycyrrhizic acid ammonium salt (2ng, 4ng, and 8
ng/ml...

Ibadullah Jan1*, Iqbal Munir2, Inamullah Khan3, Syed Muhammad Suhail4 and Aqib Iqbal2

...al model. Different techniques such as xylene induced ear edema model, Carrageenan induced paw edema model, Hot plate pain model, Ethanoic acid-induced pain model, Yeast instigated pyrexia model acute toxicity model was used to investigate Dosiflavone. Dosiflavone showed significant (p<0.05) reduction of the ear edema at a high dose. However, at 20 mg/kg for 60 minutes, the impact was less important. In vivo anti-inflammatory testing revealed that Dosiflavo...

Mian Ibad Ali Shah*, Yasir Saleem Afridi and Laiq Hasan

...tion of controlling techniques for new cases using controllable factors have been provided. The results have been achieved using descriptive analysis of the time-series data. The goal was to check the effect of various parameters (like new tests, government implemented lockdown index, etc.) on the new/ active COVID-19 cases, so policies can be devised accordingly to control the spread of the disease. The data was first converted into summary numbers, followed ...

Iftikhar ud Din* and Yousaf Hayat

...ent and appropriate techniques for analyzing multiple traits simultaneously, is the multivariate analysis of variance (MANOVA). This study deals with the illustration of MANOVA using simulated data, related to agricultural trials. The appropriate design, methods of analysis, interpretation and conclusion are carried out on simulated data. For illustration three factors are considered in a factorial arrangement in completely randomized design (CRD). For the two...

Hina Afzal*, Sarfraz Hassan, Muhammad Khalid Bashir and Asghar Ali 

...e growth accounting technique to obtain reliable estimates of total factor productivity. The study covered total factor productivity growth period from 1973-2020. Results indicated that the overall economy average TFP growth rate is higher from 1981 to 1990 in the agriculture sector of the country. The TFP growth of agriculture sector was higher in this decade. Comparison between the TFP with other sectors like industrial and services, having 1.51%, 4.01% and ...
Wenjuan Yan1, Qunlan Zhou2, Bo Liu2,3*, Cunxin Sun2, Huimin Zhang3 and Changyou Song2
...f Isaria cicadae Miquel (I. cicadae) on growth performance and meat quality of giant freshwater prawn (Macrobrachium rosenbergii). The prawns were fed with five isonitrogenous (40.54% crude protein) and isolipid (6.16% crude lipid) diets with different I. cicadae levels (0, 100, 200, 400, and 800 mg Kg-1). Results revealed that I. cicadae significantly increased specific growth ratio (SGR) and protein efficiency ra...

Zain Ullah1*, Anwar Ali Shad1 and Saqib Ali2

...1D, and 2D NMR data techniques. The antibacterial assays of the obtained six oil sub-fractions (H-1 to H-6) were accomplished against S. epidermis, E. coli, and S. aureus bacterial strains and the results ranged from 10-18 mm zone of inhibition. The GC-MS analysis of the six oil sub-fractions (H-1 to H-6) confirmed the existence of linoleic acid, palmitic acid, and oleic acid in the highest amount.

...

Sana Javeid* and Khalid Nawab

...ple random sampling techniques. The data were collected using an interview schedule; analysis were carried out by using cross-tabulation, frequency counts, percentages through Statistical Package for Social Sciences (SPSS). To measure the poverty level of apple orchard growers, poverty score card was employed. In addition, bivariate analysis was used to examine relationship between various extension practices and poverty. Results of the study revealed that maj...

Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1

...osiphon aristatus Blume Miq) is empirically used in Indonesia, Malaysia, Australia, and Southern Asia as an antibacterial, antidiabetic, antihypertensive anti-inflammatory. The country of Indonesia has three varieties of O. aristatus, namely purple, white-purple and white. The purple and white-purple population has started to decline. Efforts are needed to maintain the two varieties’ population, one of which is plant tissue culture. It is hoped that in v...

Moheem Khan1, Arif Ali1*, Shafique Ahmed Memon1, Taimoor Khan Qambrani2, Ghulam Khaliq3, Jahanzaib Khan1, Saghir Ahmed1, Azmat Hussain Abro4 and Sohail Azeem Baloch1

... light is a helpful technique for the management of T. castaneum because we found that all developmental stages of T. castaneum showed susceptibility on UV-B light that may be useful for the protection of stored grain products from T. castaneum infestation. 
...
Pir Meher Ali Shah1*, Shahryar Shafique Qureshi1, Rizwan Aslam Butt2, Haider Ali3 and Shahid Khan4
 
... multiplexing (WDM) techniques are employed to ensure high capacity. Furthermore, free space optical (FSO) links are employed at the distribution level (DL) to facilitate dense deployment of RRUs near the subscriber’s premises. The proposed model is analyzed in Optisystem, and it is observed to support 40 Gbps of data, over 4 km length of FSO.  

...

Hina Fatima1*, Bushra Yasmin2 and Lal Khan Almas3

...d intensive farming techniques to attain self-sufficiency in production. This study conducted the efficiency and productivity analysis by applying the parametric and non-parametric techniques to evaluate the optimal utilization of farm resources in multiple cropping system and the efficiency gains from tunnel technology. The data were collected from 150 multiple bitter gourd-capsicum cropping system farms. The empirical find...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
...ed using the F-MSAP technique, including brown adipose tissue (BAT), brain (B), heart (H), kidney (K), liver (L), and muscle (M) tissues. An average of 67.0% CCGG loci was methylated in each tissue. There were significant methylation differences among tissues in full-methylation (p < 0.001) and total methylation (p = 0.001). Moreover, various unique methylated loci (15–59) were also detected. Non-negli...

Kawa A. Ali1*, Sazar S. Noraldeen2 and Arshad A. Yaseen2, 3

...orophyll estimation techniques for two vegetable crops (aroma tomato Solanum lycopersicum L. and green pepper Capsicum annuum L.) in three different environments (open field, lath house and plastic house) to indicate the best method of chlorophyll estimation. After 80 days from sowing chlorophyll estimation was implemented first by nondestructive methods using SPAD (SPAD-502 Plus Konica Minolta Tokyo, Japan) and atLEAF chlorophyll meter (at-LEAF FT-GREEN-LLC W...

Khaliq Dad1, Muhammad Nawaz2*, Muhamamd Ibrahim3, Fengliang Zhao4, Rumsha Hassan2, Humaira Nawaz5, Muhammad Usman Saleem6, Kinat Javed2, Ayesha Komal2 and Hajra Naz2

...ironmental friendly techniques. 

...
Arsalan Khan1*, Navid Anjum2, Muhammad Shuaib Khan3, Shakeeb Ullah3
Ali Zaman3, Kamran Safdar3, Khalid Muhammad3, Ambrina Tariq4
Muhammad Inam Malik3, Muhammad Kamal Shah3, Atiq ur Rehman3 
and Hamza Maris3
...factory alternative technique is the immunological control against ticks infestation. Therefore, this study was planned to investigate the effect of crude extract of hard ticks and its application as a vaccine against hard ticks of the cattle. For this purpose, ticks were collected from cattle and crude extract of ticks was prepared using standard procedures i.e. mixed with formalin, antibiotic and vegetable oil to prepare the vaccine. This vaccine was ...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...ard microbiological techniques, and API 20E and for final confirmation multiplex PCR was performed using genus and species-specific primers. The susceptibility to various antimicrobial agents including the aminoglycosides was determined by the Kirby-Bauer method and minimum inhibitory concentration (MIC) to aminoglycosides was evaluated by the broth microdilution method. Moreover, the armA genes were studied by PCR followed by Sanger sequencing. The iso...

 Yanqiang Zhou1, Lixin Wang1, Chunxiao Hao1, Xiuyun Li2, Shakeel Hussain1, Dongdong Shen1, Zhiwei Peng1, Qi`an Zhai1 and Zhijun Hou1,*

...chloride floatation technique. The microscopic analysis, based on the morphology of oocysts, indicates that the present parasites are Capillaria sp. in blue wildebeest; Eimeria christenseni, E. alijevi, Trichuris sp. and one Strongy-type species in goats; Trichuris sp., Nematodirus sp., Moniezia sp., E. macusaniensis, another Eimeria sp. and another Strongy-type (different with goats) in alpacas. It was d...
Bushra Shaikh, Sadia Muniza Faraz*, Syed Riaz un Nabi Jafri, Syed Usman Ali
...n and path planning techniques. A map matching algorithm based on matrix matching is being proposed in this paper. The localization of robot is determined by the path acquired during the random maneuvering on the grid based maze, whereas, the shortest path to the target position is determined by estimating x and y coordinates. The algorithm interprets the map of the environment, expressed by grid based model and generates an optimal or near optimal path. The p...

Muhammad Yaseen*, Muhammad Adnan, Muhammad Luqman, Muhammad Zeeshan Majeed and Muhammad Umer Mehmood

...multistage sampling technique. Statistical Package for Social Sciences (SPSS) was used to have descriptive statistics of data. The majority of youth respondents were taking part in agricultural activities like land preparation, sowing practices, irrigation, harvesting and storage obtaining a percentage of 80.2%, 77.6%, 79.3%, 90%, and 75.8% respectively. Lack of basic farming knowledge and skills is the topmost factor hindering youth involvement in agricultura...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*
...CR and Western blot techniques were performed to determine the expression levels of apoptosis-related genes including Bax, Bcl-2 BAG-1, BAG-3 and STAT3 in H9N2 infected erythrocytes of chickens for incubation time intervals of 0, 2, 6 and 10 h. The expression of all the genes related to apoptosis were significantly up-regulated at different time periods, except of Bcl-2 which was significantly down-regulated at 6 and 10 h of infection. To conclude, there are a...

Milkessa Wakjira, Fikiru Temesgen*, Chala Hailu and Dabesa Wegari

...multistage sampling techniques was employ to select 372 MSE in six district towns of Western Shewa Zone by administering personal interview. Descriptive statistics such as percentage, frequency, maximum, minimum, mean and standard deviation were used to measure the association and growth probability of MSEs. The major problems finding in the study area were facing multiple issues such as increase in cost of production, shortage of workers and reduction in sale...

Kemi Funmilayo Omotesho1*, Philip Akintunde Fatodu1 and Toyin Benedict Ajibade2

...age random sampling technique was used to select 178 youth farmers on whom a structured interview schedule was administered. Data was analyzed using descriptive statistics and the Multiple Regression Analysis. Results reveal that most of the respondents were male (73.6%), with mean age of 28 years and average of 12 years farming experience. Farmers’ level of record keeping was low (Mean=2.26), and their attitude was poor (index=0.43). Farmers’ know...

Malik Muhammad Shafi, Rabia Habib and Haidar Ali*

...ugh random sampling technique, a sample size of 90 households from 452 farm households were taken. Total consumption expenditures, total income, and land size were the variables which showed significant results. Because their overall p-values indicates significant results at 5% significant value. Results of these variables revealed a positive relationship between household (farmers) savings and total income with coefficient 0.332143 and P-value 0.00*** and lan...

Saadu Mustapha1,2, Norsida Man1*,  Jasmin Arif Shah1, Nitty Hirawaty Kamarulzaman1 and Ahmadu Abubakar Tafida3

...ge cluster sampling technique, using organized questionnaires administered to 254 respondents. Descriptive and inferential statistics was used. The research used Rogers’ Theory of Innovation Diffusion, Rogers and Burde’s Theory of Social Change. The findings from the study have shown that GSM, radio, television, camera, internet, computer, CD/DVD player and VHS video are the most commonly used ICT components in various aspect of extension work. The...

Inam Ur Rahman1, Sanam Wagma Khattak2*, Muhammad Israr3 and Saima Hashim4

...ough linear scaling technique. The study has developed two thresholds each one for overall developing countries and regional countries (South Asia). The food security situation of Pakistan is compared to both thresholds. The results show that Pakistan is comparatively a food insecure country. It lags behind in terms of food availability and utilization that makes the overall food security scores lower. The study suggests that food security situation in Pakista...

Tajnees Pirzada1*, Weenghar Ali Chandio1, Mansoor Ali Kalhoro2, Mir Munsif Ali Talpur1, Waheed Ali Mirbahar1, Abdul Ghafar Solangi1, Zulfiquar Ali Jumani1 and Rehana Kerio1

...ous physicochemical techniques such as X-ray diffraction, Fourier transform infrared (FTIR), UV-Vis spectroscopy, and scanning electron microscopy (SEM).The data confirmed the adsorption of humic acid on the surface of ZnO nanoparticles. The HA/ZnO NPs exposure significantly increased germination by 83 %, root length (60 %), shoot length (78 %), fresh weight (71.4 %) and dry weight (45.9 %).. The HA/ZnO NPs were applied as co-fertilizer to upgrade the Brassica...

Muhammad Yaseen1*, Muhammad Sallam Shahzad2, Farhat Ullah Khan2, Muhammad Luqman1, Usman Saleem3 and Shoaib Nasir4

...ulti-stage sampling technique was used for this study; firstly, one tehsil out of three tehsils i.e. Narowal, Shakargarh, and Zafarwal was selected, secondly, 6 union councils were selected, and then two villages from each union council and 10 farmers from each village were selected randomly. Thus, a total sample comprised of 120 rice growers. A well-structured interview schedule was used for data collection. Descriptive statistics i.e. frequency, mean and sta...

Thomby Paul, Sreekanta Biswas, Sabiha Zarin Tasnim Bristi, Debashish Sarker, Saroj Kumar Yadav and Bhajan Chandra Das*

...nal fixation (ORIF) technique was performed to repair the olecranon fracture using two Kirschner wires and tension band wire, and immediate radiographs were taken to confirm the position of implants. On 30th day of post-surgical observation, mediolateral radiograph revealed migration of implants but callus formation was observed between the fracture ends of olecranon in order that the implants were removed. On 45th day of clinical observation, the dog showed g...
Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and 
Carlos Alfonso Alvarez-Gonzalez4
... most commonly used techniques, due to its precision, sensitivity and high performance, particularly in gene expression to compare between cells, tissues and organs; as well as different populations, stages of development, metabolism, among other conditions. This study analyzed the stability and normalization of six commonly used reference genes such as alpha elongation factor (ef1-α), beta-actin (actb), 18S ribosomal RNA (18s rrna),...
Shoaib ur Rehman1, Jabbar Khan2*, Raaza Malja Khan3, Maimoona Azam4 and Zeeshan Mutahir5
...reaction (ARMS-PCR) technique, all samples were analyzed for the most common β-thalassemia mutations reported in Pakistani population. The most common mutations detected in karak region were frameshift codons (FSC) 8/9 (þG) (HBB: c.27_28insG), followed by IVS-I-5(G>C), FSC 5 (–CT) and Codon 15 (G>A). The present study hence showed differences with previous results from other regions of the Pashtun ethnic group, which demarcates the heter...

Dilshad Ahmad1*, Muhammad Afzal2 and Asif Ali Abro3

...uction. An economic technique of log-linear regression model was applied to identify the factors influencing dairy production and estimate household income difference from dairy production due to formal credit from Zarai Taraqiati Bank Limited (ZTBL). Estimates of the study indicated that agricultural credit facilitated to enhance household income 23.58%, increases herd size 35.5% and raises the average number of milking animals 39.74%. The elasticity of milki...

Ayesha Khan1*, Asmatullah2, Urooba Perviaz1, Khalid Nawab1 and Mahmood Iqbal1

...ilizers. One of the techniques for organic farming is EM-Technology. EM-Technology is used in addition with animal manure and other organic wastes effectively in agriculture. The universe of the study was Malakand division of Khyber Pakhtunkhwa. The EM-Technology using 128 farmers’ data were gathered from the District Director Agriculture of six districts. Each farmer was interviewed face to face to collect accurate information of EM-Technology used thro...
Nasir Ali Tauqir1, Asim Faraz2*, Annamaria Passantino3, Muhammad Asif Shahzad1, Rana Muhammad Bilal4, Adeel Tahir1, Hafiz Muhammad Ishaq2 and Abdul Waheed2
...influence of corn steep liquor and enzose mixture as a non-conventional protein ingredient in day old broiler chicks. For this purpose, 42 days study was conducted using hubbard broiler chicks (n=200). The birds were divided into 5 groups containing 4 replicates having 10 chicks each. Birds were offered 5 iso-caloric and isonitrogenous broiler starter (metabolizable energy 2850 kcal/ kg; crude protein 20%) diet from day 1-21 and broiler finisher ...
Shabana Naz1*, Nishat Ali Khan1, Arnab Tanweer1, Shifa Moazzam1, Qudrat Ullah2, Farkhanda Asad1, Irfan Khattak3, Sajida Batool4 and Rifat Ullah Khan5
...squo;s egg counting technique. The results showed that 21 peafowl (8 males and 13 females) were infected with Eimeria sp. (66.33 %), Ascaridia sp. (3.33 %,) Strongyloides sp. (3.33 %), Ascaris sp. (6.67), Heterakis sp. (3.33 %), and Hymenolepis sp. (10 %). The overall prevalence of gastrointestinal parasites was 70 % having EPG 9150. The efficacy of Lemavisole (97...
Iqra Mobeen1*, Rabia Arif1*,Maimoona Ilyas1, Siu Fai Lee2 and Muhammad Saleem1
... A subset of SNPs were unique in SFS strains and others were fixed in the NFS strains of S. fimicola. These polymorphisms might be adaptive in stressful environmental conditions. Genotyping of eight SNPs of RK-MTases genes of S. fimicola was accomplished by designing allele specific primers via amplification refractory mutation system–PCR (ARMS-PCR) yielding amplicons of different sizes. Th...
Farah Deeba1, Hafiz Abdullah Shakir1, Muhammad Irfan2, Muhammad Khan1 and Javed Iqbal Qazi1*
...ence identification technique. B. subtilis yielded highest chitinase on CPM at pH 7 with 3% inoculum size after incubating at 37 °C for 3 days. Plackett-Burman design was used for screening of medium components. The optimization of concentration of significantly impacted medium components for chitinase production was carried out using central composite design of response surface methodology. The maximum chitinase production was achieved employing 5%...

Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2* 

...abbits by molecular techniques. To establish this goal, lungs, liver, and heart (50 for each organ) were collected from 50 diseased rabbits (suffering from respiratory signs) from backyard rabbits at different localities in Sohag governorate, Egypt. All samples were submitted for PCR test and other conventional methods of identification. Recovery of P. multocida isolates of diseased rabbits from lung, liver, and heart were 23 (46%), 11 (22%), 13 (26%), respect...
Longjun Guo*, Jiaqi Teng, Juan Wang and Yukun Chen
...the two groups. DTI technique was taken to compare DTI anisotropic diffusion coefficients of the two groups. According to our results there was a statistically significant difference in cerebrospinal fluid Tau protein concentration between the experimental group and the control group (P<0.05), and there was no statistically significant difference in CSF IgG index between the experimental group and the control group (P>0.05). DTI was used to...

Tilahun Woretaw1, Netsanet Beyero2*, Yonatan Kassu3 

... Purposive sampling technique was used to select two agroecology (midland and highland) and five representatives (two from midland and three from highland) kebeles based on the coverage of P. schimperiana. A total of 100 respondents, 58 from three high land peasant associations and 42 from two midland peasant associations, were purposely selected for an interview who had at least one livestock. Secondary data sources, field observation, and semi-structured que...

Arshad Farooq1, Abdul Hassan1, Muhammad Ishaq2, Asif Nawaz3*, Iltaf Ullah4 and Hidayatullah5

...eties (26.67%), IPM techniques (25.84%), wheat on ridges/seedbed (24.17%), heat and drought tolerant maize (OPVs) varieties (20.84%), heat and drought tolerant wheat varieties (20%) and organic farming (16.67%). Knowledge index revealed that both districts’ farmers had 35.71% knowledge level in fourteen different indicators in the study area. The farmers of district Charsadda had slightly high knowledge level (38.67%) compared to district Nowshera farmer...

Asad Ullah1*, Umar Sadique2, Ibadullah Jan2, Imad Khan1, Raheela Taj3, Mumtaz Ali Khan4, Salah-ud-din4, Naimat Ullah Khan1

...M bovis through PCR technique. A significant association (p<0.05) was found between livestock farm workers and abattoir workers with the prevalence of zoonotic tuberculosis. The level of knowledge and awareness about the zoonotic bTB were very low and considered high-risk occupational groups of gaining zoonotic TB. Bovine tuberculosis is a main public health issue in different job-related groups in district Peshawar, Khyber Pakhtunkhwa, Pakistan. The survei...

Prima Mei Widiyanti1*, Mirnawati B Sudarwanto2, Etih Sudarnika2, Raphaella Widiastuti1 

... using high performance liquid chromatography (HPLC) method. This study showed that no heating (control), heating at 72 oC for 15 seconds (high temperature short time pasteurization), and 89 oC for 1 second (higher heat shorter time pasteurization) did not affect on enrofloxacin antibiotic residue concentration, however heating at 121 oC for 15 minutes (sterilization) affected the antibiotic enrofloxacin. The stability of enrofloxacin antibiotic could result p...

Nedžad Hadžiomerović1*, Ozan Gündemir2, Senad Kovačević1 

... facial skeleton has a unique morphological characteristic and importance for the taxonomy. This study aimed to investigate size and shape differences of the mandible of the red fox and golden jackal. The research included 17 adult red fox and golden jackal mandibles from B&H. The morphometric analysis was performed on the 11 measurements and geometric analysis evaluates shape variation between two species with ten landmarks. The study revealed that the ma...

Ali Zohaib*, Muzzammil Hussain, Iftikhar Ahmad and Adnan Bashir

...uite a new planting technique for rice. However, optimization of seeding rate for producing mat type nursery is crucial to acquire suitable seedlings and planting density for better yield of mechanically transplanted rice (MTR). Current 2-years field study was conducted to determine effect of seeding rate (80, 90, 100 and 110 g per tray) for preparing nursery mats of fine basmati rice on plant growth, grain yield and economic benefits. Seeding rate significant...

Rehman Shahzad1, Saba Irshad1* and Faisal Amin2

... of H9N2 isolates. Two unique substitutions, Gln39Arg and Lys47Glu have been noted in NA stalk domain of all isolates of this study which might be indirectly enhancing the virulence of virus. These findings demonstrated that new mutations are emerging in B2 sub-lineage and there is need for constant surveillance of evolving genome of H9N2 virus prevailing in the country to combat the future challenges of avian influenza out breaks in Pakistan.

...
Amjad Ali1, Muhammad Amir2*, Bilal Ur Rehman2, Zafar Khan3 and Abid Munir1
 
...g, image processing techniques include: low-quality-fingerprint enhancement, normalization, binarization and thinning of fingerprint image followed by feature extraction. In this paper, a novel encryption method for minutiae based low-quality-fingerprint framework is proposed. The proposed encryption algorithm works through addition of spurious information to the originally extracted fingerprint data. The addition of spurious information is in correlation with...
Lin Ye, Jie-Lan Jiang*, Jia Xu, Rui-Ting Liu, Yin-Qiu Tian, Qing-Qing Zang, Jia-Yi Cao and Ming-Guang Mao*
...iption PCR (RT-PCR) technique. The results showed that TrCLCN5 cDNA contained a complete open reading frame of 2505 bp continuously encoding 834 amino acid residues. Sequence alignments revealed that the TrCLCN5 cDNA is highly homologous (96.2% and 96.1%) with that of Takifugu bimaculatus and Takifugu flavidus. SignalP and TMHMM analysis showed that TrCLCN5-deduced protein was a type III transmembrane protein and lacked a typical signal peptide. Conserved Doma...

A.M. Abdul Azeem, Ashraf M. Mounir and Amr N. El-Shahat*

...n be used as useful technique to improve the antioxidant activity of PSDP without loss in its quality characteristics. Also, the results suggested the effectiveness of GPSDP as a natural antioxidant that can reduce diabetic disorders.

...

Xiaoli Cai1,2, Saeed Ahmed3, Zhixin Lei1,*, Jianping Wu1,* and Taolei Sun1,2,*

.... The nanoparticle has unique physical and chemical properties, a large surface area, and an electric charge. Additionally, its antibacterial drug encapsulation can make it easier to enter the cell and improve the concentration in the target tissue. In the current review, the purpose is to find a more effective nanometer system to fight against intracellular bacterial infection. Current observation describes the drug resistance mechanism of bacteria, the bacte...

Irshad Ahmad1,2*

...so include cultural techniques comprising in-groove humidity and palm density, judicious use of insecticides, using biological control agents including entomopathogenic fungi, nematodes as well as adopting the regulatory methods of domestic and international quarantine. This paper provides updated information about IPM, which look like a promising paradigm that can be adopted for the control of red palm weevil.

...

Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

...h a semi-nested PCR technique.

Keywords | ELISA, PCR, 16srRNA 

...

Mahreen Alam*, Muhammad Ashfaq, Sarfraz Hassan and Asghar Ali 

...ied random sampling technique was used to collect data, and three stratums like head, middle, and tail of 11-L distributary were selected based on groundwater quality. The results showed that the benefit-cost ratio for milk production was higher with good groundwater quality. The Data Envelopment Analysis was used to calculate the technical efficiency. It found that farmers with good groundwater quality were technically more efficient. Tobit model used to anal...

Muhammad Abdullah1, Sana Zahoor1*, Akhtar Ali2 and Masroor Ellahi Babar3

...nd -308 by ARMS PCR technique in asthmatic and control group. It was a prospective study constituting 17 clinical diagnosed asthmatic patients (Diseased) and 19 non-asthmatic patients (Control). The female was 6 (31.6%) and males were 13 (68.4%) in control group while females were 9 (52.9%) and male were 8 (47.1%) in asthmatic group. Mean age (49.59±15.82 year) and BMI (21.96±06.67 kg/m2) were recorded for asthmatic group while mean age and BMI f...

Chukwudi Njoku1*, Inyang Etim-Inyang2, Prince-Charles Itu1 and Arinze Uzoezie1

...ia Evaluation (MCE) technique was applied using secondary data and considering factors such as elevation, land-use, accessibility (roads), river width, population density, distance from anthropogenic activities and climatic variables (temperature and rainfall intensity). The criteria and threshold for siting Tilapia cage culture were adopted from previous studies and processes such as classification, buffering, interpolation, slope analyses, single and multipl...

Saba Iqbal, Asmat Ullah*, Muhammad Luqman, Hafiz Muhammad Akram, Muhammad Kashif Munir and Nawal Zafar

...l to explore useful techniques in crop production that utilize less water without compromising yield, sacrificing income of the farming community and evidencing sustainability in production systems. Thus, a two years field study (during 2017-18 and 2018-19) was conducted at research area of Agronomic Research Station Khanewal, Punjab, Pakistan to compare the existing traditional winter wheat (Triticum aestivum L.) planting techniq
Anjum Ara1, Usman Ali1, Muhammad Furqan2*, Zulfiqar Ali2
Muhammad Mudassar Shahzad3, Khawaja Basharat Ahmed4
Riaz Aziz Minhas4, Waqar Ahmed2, Zakir Hussain2 and Mian Aman Ullah2
...s and cruel fishing techniques are serious issues for their survival while anthropogenic activities (10%) are responsible due to destruction of habitat. Limited awareness was observed among local inhabitants regarding importance, ecological role and legal status of freshwater turtles. Awareness and strict law enforcement is recommended to reduce illegal turtle trade and conserve these important species.

...

Saydat Saad1, Doaa Abdel-Fattah1, Tarek Khamis2, Aya El-Sobky1* 

...polis using various techniques, nano-propolis can be more effective without compromising its qualities. Propolis has numerous benefits, including anti-inflammatory, antioxidant, anticancer, and antifungal properties. Low bioavailability, solubility, absorption, and untargeted control, the advantages of free form propolis are limited due to previous reasons. Nano-propolis is made using a variety of nanoencapsulation processes. Because of their smaller size, nan...

Sozan A.A. Ismaeil1*, Hassan Emam2 

...cted from streets at Shariq province. The morphological findings showed that the liver of two species of birds is located in a hepato-peritoneal cavity, and each is composed of two undivided lobes, a large right lobe and a small left one which have light brown to dark red in pigeon and dark red in ibis. The histological structure of the organ in both species was different, were consisted of several lobules that were not separated from each other by trabeculae ...

Hussain Ali1, Shahid Sattar Khan1, Fazal Maula2, Said Hussain Shah3* and Misbah Uddin1

...chemical management techniques for rice stem borers.

...

Shoaib Hameed1, Shakeel Ahmad1, Jaffar Ud Din2 and Muhammad Ali Nawaz3*

...ea. Multiple survey techniques questionnaire surveys, sign surveys and camera trapping were used during the period 2008–2010 in five study blocks delineated on natural watersheds in Pakistan’s Chitral district. Based on questionnaire surveys, sign surveys and direct sighting, Himalayan brown bear presence was confirmed only in the Yarkhun and Laspur valleys. Ninety-six respondents (59 from Laspur Valley and 37 from Broghil Valley) reported a total ...

G. Basana Gowda*, N.B. Patil, M. Sahu, S.R. Prabhukarthikeyan, S. Raghu, G.P. Pandi, T. Adak, C.K. Swain, S. Pokhare, S.D. Mohapatra and P.C. Rath 

...mical and molecular techniques. Among nine isolates of bacteria from resistant and susceptible populations, six isolates belonged to gram positive bacteria and three belonged to gram-negative. The 16S rRNA gene sequences displayed 96 to 100 per cent homology to other 16S rRNA gene of strains within the National Centre for Biotechnological information (NCBI), Genbank. Among different bacteria strains, two, Bacillus subtilis and Staphylococcus saprophyticus were...

Mirza Lena1,2, Dinda Fathia Syahramadani2, Alfi Nurrachma Gustya2, Arif Darmawan2,3, Sumiati2, Wiwin Winarsih4, Minoru Maeda5, Komang Gede Wiryawan2*  

...CFU/g, and T6 = B. amyloliquefaciens spore at 109 CFU/g. At the pre-starter, the dietary combination T4 yielded lower (p < 0.05) feed conversion ratios compared to the T6 group. Broilers fed L. lactis showed higher body weight gains (p < 0.05) than those fed T5 and T6 groups. All treatments had no significant effect on broiler performance in starter, grower, cumulative periods, as well as E. coli and lactic acid bacteria populations. The combination of L...

Hafiz Adnan Ahmad1,2*, Muhammad Aslam2, Salman Gul2, Tariq Mehmood2 and Munawar Ali Munawar2

... with spectroscopic techniques and further evaluated for anti-inflammatory potential. The synthesized hybrids were screened in vivo by employing carrageenan-induced paw edema method at 10 mg/kg dose. Among all derivatives, two compounds 4g and 4b displayed better anti-inflammation potential than standard drugs celecoxib and indomethacin. While the anti-inflammation profile of compounds 4h, 4e and 4f during in vivo screening was also comparable to celecoxib and...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...s for 4 h and frozen in liquid nitrogen at -196 °C. The parameters evaluated were percentage motility, plasma membrane integrity (HOST assay), acrosomal integrity (NAR), viability (Live/Dead), DNA integrity (Acridine orange assay) and oxidative stress (TBARS assay). Five straws from each Gallic acid group were thawed individually in water bath at 37°C for 30 seconds. The addition of 15 µM GA to semen extender improved marginally the buffalo bull ...

Nargis Sardar1*, Maria Binte Sarfraz2, Sufian Rasheed3 , AKM Rezwan Sardar4 , Fahamida Zaman5, Arsalan Rasheed6,7* 

...l as quick and easy technique for its determination. This review focuses on the Vitamin D investigations of electrochemical biosensors that have been conducted in recent years. According to the research, the practical use of electrochemical biosensors is attributed to the existence of UV radiation and transactivation of pharmaceutical items, food, or even human blood plasma in the detection of Vitamin D from diverse samples, including Vitamin D production in n...
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
... using High Performance Liquid Chromatography. The results revealed that the incidence of tetracycline residues was 260 (87%) in all examined chicken meat. The average concentration values of oxytetracycline (OTC), tetracycline (TTC), chlorotetracycline (CTC), and doxycycline (DOC) in broiler samples were 160.26, 89.19, 98.75, and 175.64µg/Kg respectively. Based on the local and international regulations, the results revealed that out of 260 positive sam...

Fatma Desouki Mohammed Abdallah

...ological data. This technique and its related techniques play an important role in many statistical methods like principal component regression.
 
Keywords | Carcass traits, Eigen-values, Japanese quail, Principal component analysis, Scree plot
...

Naila Shahzadi1, Hafiz Muhammad Tahir1*, Shaukat Ali1, Muhammad Farooq Bhatti2, Azizullah1, Shafaat Yar Khan3, Abdul Khaliq4

...s, B. subtilis, B. amyloliquefaciens, B. licheniformis, Lactobacillus casei, Saccharomyces cerevisiae and Spirulina), vitamins (C and E), royal jelly, ascorbic acid, cowpea seed powder, AgNPs, secondary metabolites (phenols flavonoids, phenolic amino acids and proline) and white hen’s egg at different larval instars. Economic parameters (pupal weight, shell ratio (%), cocoon weight, filament length, shell weight, denier, fibroin and sericin contents) and...

Nora Faten Afifah Mohamad1, Hassan Haji Mohd Daud1, Ruhil Hayati Hamdan2, Sharifah Raina Manaf3, Ain Auzureen Mat Zin2, Nik Nur Fazlina Nik Mohd Fauzi2

... were performed using a liquid gas chromatographic examination of methyl esters. The overall mean of saturated fatty acid (SFA) composition was significantly higher (p<0.05) in C. macrocephalus (48.21 ± 5.11) as compared C. gariepinus (32.15 ± 1.23) and hybrid CMxCG (37.70±0.35). Total monounsaturated fatty acid (MUFAs) composition of C. macrocephalus, C. gariepinus, and hybrid CMxCG was 32.14 ± 5.86, 45.24 ± 3.21, and 30....
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...n reaction (RT-PCR) technique and gene sequencing of partial portion of VP2 gene. The two isolates (Genbank accession numbers MW925051 and MW9250522) were characterized as very virulent IBDV (vvIBDV). The obtained nucleotide sequences of partial portion of VP2 gene of 2 isolates revealed 97.0 - 100% and 91.2-92.5% identity with the Egyptian strains and vaccine strains respectively. Finally, significant genetic changes in our IBDV isolates were observed as com...

Safaa M. Barghash1*, Amani A. Hafez2

...oma evansi is the most ubiquitous protozoan parasite with a wide geographical distribution. The current study aimed to investigate if Trypanosoma evansi induces immunosuppression that may interfere with the development of immunity after vaccination in rats against Pasteurella multocida. T. evansi-infected and non-infected Wester Albino rats immunized against pasteurellosis with two vaccines; one is commercial, and the other is a formalin-killed vaccine (prepar...
El-Kholy, K.H.1*, Tag El-Din H.1, Seham N.E. Seleem1, A.M. El-Shhat2
...o;s eggs could be a technique to boost hatchability and ducklings’ quality. The objective of the present study was to determine the effect of in-ovo dipping of SV on the hatchability%, embryonic mortality, and internal and external of newly hatched ducklings. A total of 1560 fertile eggs used in the current experiment were obtained from Sudani ducks flocks of 33 weeks of age. The eggs were randomly distributed into of five different SV solution concentra...

Ahmed Abdel-Rady1,2, Walaa Mostafa3* 

...smear and flotation techniques were done to determine the presence of the eggs. Out of these, 104 animals were infected with strongyle eggs with (26%) prevalence. Sex, season, and age were the factors that affect the prevalence of trichostrongyle infection in this study. Data analysis reported that there was a significant effect for the season and sex on the trichostrongyle infection level; the highest nematode infection level was observed in the cold months (...

Abid Hussain1*, Saifullah Khan2, Shamas Liaqat3 and Shafiullah4 

...istan. The regions are unique in topographical and agricultural perspective from other areas of the country. Cultivated area is just 0.57% of the geographical area. Operational per capita land holding in the region is just 0.05 ha (less than 500 m2) which is insufficient to produce sufficient amount of food to feed a person throughout the year. The arable land in the region is 1.37% of the geographical area. Thus, production base of the region can be almost do...

Ejaz Ali and Nageen Hussain*

...akistani population is unique for the study of recessive genetic diseases due to a higher rate of consanguinity and Hearing Loss (HL) is one of them that affects more than 466 million people worldwide; will increase to 900 million by 2050. GJB2 mutation is one of the main causes of hearing loss in different populations, including Pakistan, that encodes a gap junction protein involved in the homeostasis of the inner ear by recycling potassium ion. This research...

Muhammad Ammar Dilawar1,2, Hong Seok Mun1, Myeong Gil Jeong1, Eun Ju Yang3, Hyeoung Seog Park4 and Chul Ju Yang1,2,*

...ergii (GT) extracts and liquid mineral (LM) in drinking water, on the growth performance, breast and thigh meat yield, digestive organ weights and meat quality characteristics in broilers. A total of 210 broilers were divided into the following treatment groups: (i) control, (ii) T1 (0.05% 4 MA: 1 GT + 0.01% LM), (iii) T2 (0.05% 4 MA: 1 GT + 0.05% LM), (iv) T3 (0.05% 4 MA: 1 GT + 0.1% LM), (v) T4 (0.1% 4 MA: 1 GT + 0.01% LM), (vi) T5 (0.1% 4 MA: 1 GT + 0.05% L...

Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*

...oining tree revealed a unique genetic cluster of HS population and a similar genetic background and close relatedness between the EM and FJ population. It is the first reported polymorphic SNP markers in M. thibetana that can be applied to non-invasive samples, and the SNPs based analysis on the population structure and genetic diversity has provided new insight into the relationships among wild populations of M. thibetana.

...

Abida Shehzadi*, Muhammad Shafique and Ahmad Ali Shahid

... in recognition of 124 unique haplotypes with diversity value of 0.996. Locus DYS385a/b demonstrated highest power of discrimination, polymorphism information content and gene diversity as 0.907, 0.854 and 0.807 respectively. Analysis of Molecular Variance (AMOVA) and multidimensional scaling plot (MDS) were also generated by using online tools at YHRD database. The data from this study could have significant importance in forensic applications, population stu...
Sajad Ali1*, Naeem Ur Rehman Khatak1, Shahab E. Saqib2 and Saleem ur Rehman1
...ulti-stage sampling technique was employed. Furthermore, to estimate wheat productivity, differences in wheat productivity and socio-economic factors between owner and tenant wheat growers, a log-log regression model and an independent unpaired sample t-test was used in conjunction with face-to-face interview schedule. In addition, a dummy variable was added to the production function to analyze the impact of tenancy status on wheat productivity. The results r...

Mubashar Ahmad, Roheela Yasmeen*, Aisha Waheed Qurashi, Muhammad Hassan Ali Shah 

...als. A non-invasive technique was used to measure the cortisol levels. Urine and faecal samples of male and female blackbucks (Antilope cervicapra) were collected from Lahore Zoo, Safari Zoo and Jallo Zoo in winter and summer season to determine the stress levels. Both faecal and urine samples were prepared and further analyzed by radioimmunoassay (RIA) kit to determine the faecal and urine cortisol levels. Higher concentrations of cortisol were recorded in bo...

Ibrarul-Haq* and Malik Muhammad Shafi

...various econometric techniques. Empirical evidence from several forest based communities across the globe suggests that NTFP’s collection contribute significantly to household’s income. A total of 410 households were randomly selected for data collection from upper two tehsils of the district Swat. Data were collected from the selected sample households using simple random sampling technique with the help of well...

Asim Faraz1*, Bernard Faye2, Cem Tirink3, Ayman Balla Mustafa4, Amal AlKharusi5, Morteza Bitaraf Sani6, Nasir Ali Tauqir7, Muhammad Arslan Akbar8, Muhammad Usman Saleem9, Rana Muhammad Bilal7, Abdul Waheed1, Muhammad Shahid Nabeel10

... This newly applied technique of forced mating could be an efficient tool to slight overcomes these issues. 
...

Arshad Farooq1, Muhammad Ishaq2, Abdul Hassan1, Muhammad Zafarullah Khan3 and Asif Nawaz3*

...cultural management techniques. Out of 203 subscribers (beneficiary farmers), sixty farmers were interviewed through interview schedule. Knowledge index was used for measuring farmers’ knowledge level. The study revealed that application of mobile phones decreased the communication gap (88%) between the farmers and agricultural extension department. The agricultural advisory services provided through mobile phones have improved the knowledge level of far...

Judith Kiptoo1*, Daniel Mutisya2, Paul Ndegwa1, Ruth Amata3, Lucy Irungu4 and Rotich Godfrey5

...ed Baermann’s technique and identification by morphological features. The findings indicated that four major genera were abundant in different citrus growing ecological zones in Kenya. The identified species were Meloidogyne incognita, Tylenchulus semipenetrans, Helicotylenchus dihystera and, Pratylenchus brachyurus. The most abundant species in all the surveyed localities was T. semipenetrans. Factor regression analysis results showed that modest rainfa...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
...ugh chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa.

...

Shuhuan Li1*, Yongheng Bo2, Youzhi Li3 and Xiuzhen Yang2

...study, high performance liquid chromatography (HPLC), a simple but potent method was used to quantify atropine residues in beef. The results indicated that the method could measure atropine residues accurately, with the average recoveries between 82.37 and 88.31 % when the additional concentration ranged from 0.5 to 5.0 mg/kg, and a detection limit of 0.25 mg/kg, providing an effective and reliable method for the detection of atropine residues in beef. 

Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4 

...CPV-2 studied, several unique and existing mutations have been discovered, indicating the emergence of CPV-2c variation in Iraq.  Genetic differentiation study based on the genetic variation on the VP2 gene has shown that (CPV-2/Sul) strain belongs to new CPV-2b and CPV-2c, however, the (CPV-2/Erbil & CPV-2/krk) strains belong to new CPV-2a. A Phylogenetic tree constructed basis on the VP2 gene revealed that the field virus sequences from the present ...

Nour H. Abdel-Hamid1*, Walid Elmonir2, Eman I. M. Beleta1, Rania I. Ismail1, Momtaz Shahein1, Mahmoud E. R. Hamdy1 

... DNA fingerprinting techniques: Enterobacterial repetitive intergenic consensus (ERIC) and Random amplification polymorphism DNA (RAPD), for genotyping the Brucella isolates, as alternative rapid tools for epidemiological tracing and investigation of brucellosis in Egypt. We recovered Brucella isolates (n=29) from different host species and governorates. The isolates were identified by the bacteriological and molecular techniq

Tariq Mahmood1*, Shakeela Ismail1, Faraz Akrim2, Muhammad Farooq1, Nadeem Munawar1 and Muhammad Raza Khan1

...y using noninvasive technique of scat analysis. Results showed grey wolf being distributed at an elevation range of 3921 m to 4282 m above sea level (asl) in the park. Scat analysis showed 7 wild and 6 domestic prey species in its diet, with approximately 47 % contribution from livestock and 53 % from wild prey. Most frequently consumed domestic prey were donkey, yalk, goat and sheep, while among wild prey golden marmot (Marmota caudata), house mouse (Mus musc...
Chinar Mustafa Mohammed1*, Thamer M. Bashir1, Omar A.M. Al-Habib2, Farhad Ramadhan1, Mohammed Bassam1
...: justify;">Taurine, a ubiquitous endogenous sulfur-containing amino acid, possesses numerous pharmacological and physiological actions, including antioxidant activity, modulation of calcium homeostasis, and antiapoptotic effects. There is mounting evidence supporting the utility of taurine as a pharmacological agent against heart disease, including chronic heart failure. This study investigated the potential protective effects of taurine, on behavior and card...
Gamalat Y. Osman1, Elham M. Hassan1*, Tarek A. Salem2, Azza H. Mohamed1
...the present work and Praziquantel (PZQ) was applied as a standard drug. FWE was chemically analyzed by the high-performance liquid chromatography (HPLC) technique. The main recorded constituents were rutin, P-coumaric, hydroxyl-benzoic acid, ferulic acid, and sinapic acid. Results of the present study indicated that FWE (0.5 ml/mouse for 10 successive days) exhibited a significant reductio...

Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby* 

... HPLC (High-performance liquid chromatography). In this research, we used polymerase chain reaction (PCR) for the detection of the mcr-1 gene, and phylogeny was performed on three isolates to detect mcr-1 gene mutations and relationships. The percentage of isolated E. coli was 25.5%. All isolates showed resistance to colistin in disc-diffusion assay, while in MIC (minimum inhibitory concentration) method 68.8% exhibited resistance. Colistin was recorded as 33....

Nur Prabewi , Supriyanto, Budi Purwo Widiarso* 

...arination is a flavored liquid that serves as the ingredients of the meat marinated, and is usually used to improve the yield and shelf-life of meat. The research aims to study the effect of herbal marination on the quality characteristics of broiler meat. The variables measured including antioxidants, protein, cholesterol, and chemical residues in meat, as well as meat tenderness and color. The study conducted with Completely Randomized Design (CRD) consistin...

Asim Zubair1*, Iffat Batool2 and Ghulam Akbar Malik3

...h. Pakistan occupies a unique position in the world as a poor agricultural country with few obvious indicators of poverty. The purpose of this quantitative study was to investigate the “function of NGOs in agriculture.” The aim of this research was to see if non-governmental organizations (NGOs) are having a good impact on Pakistan’s agriculture industry. The study used simple random sampling procedures to select 160 respondents as the study&...

Naseem Sharif1,2*, Imran Muhammad Siqqique2, Muhammad Kashif Raza3, Urwa Irshad1, Muhammad Ikhlaq Khan4, Ammara Noreen4 , Muhammad Ahsan Qureshi1, Mohsin Abbas5, Muhammad Maaz Aziz5, Sitwat Riaz5, Komal Aslam5 and Naseem Akhtar6

...etic resources of this unique jujube specie.

...

Iftikhar Alam1*, Muhammad Ali2 and Muhammad Farooq3*

...tovoice-elicitation technique engaging students of different age categories, both male and female, from a variety of study disciplines at Bacha Khan University, Charsadda, KPK, Pakistan. Data on food and physical activity were collected using a set of food and lifestyle photos, which were intended to describe various situations of on-campus food and active lifestyle environment. Students identified support and barriers for healthy eating. They also identified ...

Hamdy Abdala Elnagar, Wael Mohamed Wafa*, Abdelaziz Mustafa Sakr, Mohammed Mahmoud Hegazy 

....889 and 0.971 for the unique measurement of age-TES and both age-leptin and age-PRL, respectively. A negative correlation was recorded between TES-leptin at all ages (r ranged between -0.656 and -0.995). The correlation between TES-PRL was significant at 1 (r=-0.701), 12 (r=-0.609), and 24 (r=-0.724) month of age. Leptin significantly correlated with PRL at 1 month (r=0.666), 6 (r= -0.659), and 24 (r=-0.804) month of age. The present results may suggest an in...

Inam Ul Haq1*, Muhammad Ramzan1, Mansoor Khan Khattak1 and Ahmad Khan2

... nutrients. Various techniques have been studied for declining the loss of nutrients. Therefore, this research was carried out in the University Research Farm, The University of Agriculture Peshawar, Pakistan to find the effect of raised seed bed dimensions and integrated nutrient management on the yield of winter wheat (Triticum aestivum) cultivar (Pirsabak 2015) under deep tillage in silt clay loam (Pedocals) soil during 2019. The levels of planting techn

Oluwaseun Adetarami1*, Oluwatoyin Olagunju2, Babatunde Adebayo Oyebamiji1, Adegboyega Abel Odeyemi3 and Sina Basil Johnson4

...s such as budgeting technique and logitistic regression were used. Results of the budgeting technique showed that each farmer of the FBS approach had a mean profit of ₦ 81,134.65 with a cost benefit ratio of 1.09. The results of logistic regression revealed that the age of cocoa farmer, level of education, extension contact, farm size and cocoa annual income are major determinants influencing the utilization of FBS approac...

Muhammad Islam1,4*, Farrukh Shehzad1, Muhammad Omar2, Abdul Qayyum3 and Rabia Siddiqui4

...ght explode the food critiques in the region. It is concluded that wheat productivity must be raise to ensure the food demands. The results of this study demonstrated that the CGREM found to be superior comparing with benchmark LRM.

...
Widiastuti Ardiansyah1,3*, Osfar Sjofjan2, Eko Widodo2, Suyadi Suyadi2, Danung Nur Adli2
..., Batu District and the liquid probiotics used contained Lactobacillus sp. bacteria in concentration of 1.4 x 1010 CFU/ml. A completely randomized design with six treatments and four replications was employed, with each experimental unit consisting of eight Mojosari ducks. The treatments consisted of T0 = basal diet, T1 = basal diet + 0.2% turmeric, T2 = basal diet + 0.8% turmeric, T3 = basal diet + 0.2% turmeric + 0.1% probiotics, T4 = basal diet + turmeric 0...
Amreen Zahra1*, Mushtaq A. Saleem2, Hasnain Javed3, Muhammad Azmat Ullah Khan4 and Abdul Rauf Shakoori4
...ombinant had 3.33% and unique recombinant form URFs accounted for 13.3% in the HIV Gag sequences. All Pol (URFs) demonstrated 40% of ratio among the isolates, and Subtype D with 6.66%. The present study shows that multiple subtypes of HIV-1 are present in the affected population. Hence, this study highlights the demand of continuous future studies on disease surveillance coupled with responsible evaluation of drug resistance in HIV-1 at higher resolution. This...
Dalia M. Aboelhassan1*, Inas S. Ghaly1, Noha E. Ibrahim2, Nermeen M. Shaffie3, Mariam G. Eshak1, Aboelfetoh M. Abdallah4, Ibrahim M. Farag1
...tention because of its ubiquitous presence in our living environment. However, it was revealed that this component has potent genotoxicity, causing various disorders to human and animal health. Hence, the present work is designed to investigate the capacity of Fagonia cretica extract (FCE) to inhibit bisphenol A-induced genotoxicity and histopathology in rats. In the genetic study, the assaying of expressions of IkBα and COX-1 genes in liver tissues, as ...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
...t immunofluorescent technique (IFT) was used for confirmation of BoHV-1 presence in CAM; the CAM with clear pock lesions showed yellowish-green fluorescence under the fluorescent microscope. The phylogenetic tree constructed from partial sequencing of gB of our isolate was divided into two clades; clade 1 BoHV-1 (BoHV-1.1 and BoHV-1.2) clade 2 (BoHV-5). Our isolate showed 100% homology to BoHV-1.1 reference strains away from BoHV-5 reference strains that were ...

Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi 

...he albumin gradient technique. From the results, it could be concluded that the motility, PMI and AI was slightly higher for Y-sperm than X-sperm regardless of semen sexing technique.

Keywords | Pesisir bull, Albumin gradient, Swim up, Motility, PMI, AI 

...

Reyad R. Shawish, Reda E. Hamed*, Zakaria H. Elbayoumi 

...king and processing techniques making it safer for human consumption.

Keywords | PAHs, Ready-to-Eat, Meat products, GC-MS, Egypt. 

...

Mostafa El-Sebelgy1*, Hanafy Madbouly2, Sabry Tamam2, Nagwa Ata1, Kawther Zaher1

 

...rate was analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) using a technique called untargeted (label-free) shotgun proteomic analysis. The resultant peptides were used along with RefSeq proteins and primers for multiple sequence alignment. Multiple extrinsic proteins [NS1, NS3 and VP3 of African Horse Sickness Virus (AHSV) and NS3 of Equine Encephalosis Virus (EEV)] were detected and at the same ...

Ochieng Onunga George*, Owuor Ndonga Millicent Florence, Mario Kollenberg  

...and hairs. In contrast, liquid parts consist of blood, dissolved solids, urine, gut contents, and wastewater from slaughter operations and floor cleaning. It has been reported that Abattoir wastes can have negative impacts on the areas surrounding them by causing pollution and public health concerns. Therefore, this research was initiated with the main objective being to evaluate the Parasitological and Microbiological Characteristics of Wastes Generated and t...
Ali Basim Abd Al-Hadi*, Tahreer Mohammed Al-Thuwaini, Mohammed Baqur Sahib Al-Shuhaib
...s of the genotyping technique identified three genotypes: CC, CG, and GG. Sequencing analysis revealed a novel mutation c. 25760691 C>G in the CG genotype. Association analysis revealed significant differences (P ≤ 0.05) between CC and other genotypes in reproductive hormone levels. Estrogen and progesterone were significantly higher (P≤0.05) and follicle-stimulating hormone (FSH) levels were lower in the CC genotype (85.10 ± 3.11) (pg/ml), (8....

Ayesha Khan*, Mohammad Tariq Aziz, Urooba Pervaiz and Muhammad Zafarullah Khan

...ilization of modern techniques can enhance agricultural productivity. ICT can increase and improve access to agricultural information which helps in knowledge sharing and creating awareness. The study focuses on farmer’s access to ICT tools for productivity enhancement in district Charsadda, the universe of the study. For selection of sample multi stage sampling method was used and respondents were selected from three villages namely Hajizai, Katozai and...

Md. Sabuj Rahman and Md. Mahmudul Alam*

...ncisional cecostomy technique in a calf treated for intestinal atresia. A 2-day-old cross-bred bovine calf was brought to Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh, Bangladesh having atresia coli with atresia ani et recti. Bypass surgery was performed using incisional cecostomy techniques. After 10 days post-surgical administration of antibiotics and non-steroidal anti-inflammatory drugs, a...

Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2

...molecular detection techniques were done using the tentative diagnosis by rt-PCR. The presence of 16 liver and spleen samples out of 20 (80%) from aborted fetuses were positive for EHV-4, but all were negative for EHV-1. Virus isolation trial for EHV-4 were done, eleven samples out of 20 (55%) on the CAM of ECE were positive. The virus glycoprotein B (gB) fragment (580pb) was amplified in selected isolates using the nested PCR (n-PCR), sanger sequencing of gB ...
Muhammad Jalees
...h alpha reliability technique with the help of Statistical Package for Social Sciences (SPSS) version 23.0. Regression has been conducted to test the hypothesis. The result of the study confirms a significant relationship exists between the variables, while the search engine optimization shows an insignificant impact on SCM profit. With the use of digital medium, the telecom industry in Pakistan can utilize its website platform to attract the customer’s ...

Muhammad Hassan Naseem1, Muhammad Mansha1*, Muhammad Triq1 and Peter C. Thomson2

...filtering effect of the liquid used in the device. The present study was conducted to investigate the effect of Shisha Smoking on haematological indices, body mass index, and lung capacity of shisha smokers. Forty shisha smokers (case group) and 40 non-smokers (control) of age ranging 17-30 years were recruited in this study with their consent. Peripheral blood samples of case and control subjects were analysed to evaluate the effect of shisha smoking on blood...

Nguyen Hai Quan, Nguyen Huu Van*, Nguyen Thanh Thuy, Vo Thi Minh Tam, Le Duc Thao, Le Duc Ngoan 

...sition and ensiling techniques of whole-plant sunflower Aguara 6. In Exp. 1, whole sunflower plant was harvested at the seeding period (SP) to measure biomass yield, chemical composition and energy values. Results showed that, fresh biomass yield was 62 tonnes/ha, in which, heads consisting of 46.8% with the highest, leave accounting for 35.5% and stems consisting of 17.7% as fresh matter with the lowest proportion. The dry matter (DM) content of the whole pla...

Hanan Saad El-Samahy, Amani Abd El-Naby Hafez, Mohamed Talat Ragab, Disouky Mohamed Mourad*  

...te rapid diagnostic technique. Exudates of swollen infra-orbital sinus were aspirated and aseptically collected from affected turkeys of seventeen flocks and examined by multiplex PCR against Escherichia coli (E. coli), Mycoplasma gallisepticum (M. gallisepticum), Mycoplasma meleagridis (M. meleagradis), Ornithobacterium rhinotracheale (ORT), Pasteurella multocida (P. multocida), and Pseudomonas aeruginosa (P. aeruginosa) . The most prevalent detected bacteria...
Muhammad Sajid Hamid Akash1*, Hina Sharif1, Kanwal Rehman2, Sumbal Rasheed1 and Shagufta Kamal3
... liver using HG-AAS technique. ATO exposed rats showed a significant elevation in serum glucose, reduction in serum insulin, rise in insulin resistance and alteration of carbohydrate metabolizing biomarkers when compared with unexposed rats. Arsenic concentration was found to be significantly high in liver tissues of ATO exposed rats. Contrarily, RSV was found to be effective in regulating normal glycemic level, insulin tolerance, and metabolic biomarkers. Hen...

Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5

...and Tetra- ARMS-PCR techniques were used to genotype rs2276886 of CXCL9 and rs5606198 of CXCL10 for all the samples. The results showed significant association (OR, 2.1 (0.99 to 4.30), p = 0.04) of A allele of rs2276886 with EPTB group as compared to the control. In genetic model analysis, we found strong association (OR, 0.4 (0.181 to 0.94), p = 0.03) of GG genotype with male with EPTB as compared to female in gender stratified dominant model. Furthermore, in...

Victoria Rankotsane Hlokoe, Thobela Louis Tyasi* 

...stepwise regression techniques were employed for the analysis of data. The association findings showed that BW had a positively high significant association (P < 0.01) with WH (r = 0.94), HG (r = 0.91), RH (r = 0.88), SH (r = 0.90), RW (r = 0.73), and a positive statistical significant correlation (P < 0.05) with EL (r = 0.47), and BL (r = 0.46) in males. On the other hand, BW had a positively high remarkable relationship (P < 0.01) with HG (r = 0.75)...

Khurram Sheraz* and Taj Ali Khan

...e signal processing technique Discrete Wavelet Transform (DWT) with different versions of Mann-Kendall (MK) trend tests for investigating the dominant climate systems characterizing the long-term time-series of the two most important meteorological parameters: mean air temperature (oC) and total rainfall (mm) for the different stations lying in the upper Indus River basin of Pakistan. The observed time series were categorized into monthly, seasonal, seasonally...

Hassan Raza1, Muhammad Zeeshan Majeed1*, Muhammad Irfan Majeed2, Mujeeb-Ur-Rehman1 and Muhammad Asam Riaz1

... direct applications of liquid insecticides which usually get off the target site resulting in unsatisfactory and short-term termite eradication along with environmental contaminations. This situation necessitates looking for more target-oriented and ecologically safer strategies such as baiting insecticides with some cellulose attractant. In this study, 5% technical grade fipronil was formulated as small beads (3.5 mm) made by a matrix composed of 65% maize c...

Ayesha Khan*, Zubair Ahmad Khan and Urooba Pervaiz

... Purposive sampling technique (sample taken on certain judgments about the population or researcher chooses who would be appropriate for the study) was employed to select three districts i.e. Peshawar, Mardan and Swabi from Agro-ecological Zone C of KP. The population of the study comprised of all extension agents of the selected district. Data was collected through a well-developed interview schedule from all Agriculture Officers and 41% of the Field Assistan...

Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid

...sisted reproductive techniques.

...

Sajjad Ali1*, Atta ur Rahman1 and Sher Ali2

...urvey (USGS). ARIMA technique is employed to get the forecasted values regarding Acreage, Yield, and Production of crops till 2050 in Peshawar valley. Remote sensing (RS) and geographic information systems (GIS) were used to analyze the satellite imageries. The analysis showed a persistent increase in built-up areas from 1990 to 2020 as the built-up area increased by 57090 hectares. However, the barren land indicates a decreasing pattern of growth, decreasing ...

Onwusiribe Ndubuisi Chigozirim*, Okpokiri Chibuzo Ikechukwu, Agu-Aguiyi Fortune Nneka and Oteh Ogbonnaya Ukeh

...multistage sampling technique was applied in choosing the 270 smallholder farmers in the studied area. Focused group discussions, interview sessions and surveys were used to obtain the required data. For the data analysis, descriptive statistics and correlation analysis were used to analyze the data. The findings revealed that the socio-economic status of rural farmers, especially income and education are significant factors influencing their access to institu...

Zayed M.A1, M.F. Shehata1, I.M. Ismail1, Mona Mohammady1, M.A. Radwan2*  

...ddition, ultrasound technique is commonly used in the livestock sector, such as a diagnostic tool and a tool in animal science and veterinary research. So, the present study was performed to study the effect of different weaning weights on growth performance, ultrasound measurements, carcass characteristics and meat quality of Barki ram-lambs. Moreover, this investigation aimed to examine the relationship among ultrasound measurements, body measurements, carca...

Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3 

...mined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose...

Naheed Zahra1*, Saira Batool1, Mubbashira Nazir1, Ghulam Akbar Malik2 and Iffat Batool3

...the random sampling technique. Research was based small landholders. The study’s main findings demonstrate that agriculture, together with livestock rearing is the primary occupation of the area’s residents. Females were completely involved in all aspects of farming. According to survey findings, only 32.5% of female respondents said they were part-time farmers, while 67.5% said they were actively involved all in farming activities. The respondent ...

Ejaz Ul Haq, Urooba Pervaiz*, Muhammad Zafarullah Khan and Ayesha Khan

... modern agriculture techniques and practices. This study analyzed off-season vegetables production in District Peshawar with the objectives to highlight the problems faced by farmers in cultivation of off-season vegetables, evaluate the yield and income of off-season vegetables and the role of extension workers in creating awareness among farming community regarding off-season vegetables. Data were collected from 60 sample respondents from three circles (Matta...
Zain-ul-Aabdin Abro1*, Naheed Baloch1, Raza Muhammad Memon2, Niaz Hussain Khuhro2 and Iram Shaikh1
...friendly management techniques for fruit flies.

...

Ghulam Qadar* and Khalid Nawab

...r by using sampling technique and well-structured interview schedule of 5-point likert scale was prepared in light of the study objectives. In addition, SPSS software V. 20.0 was used to analyze the research data. Principal Component Analysis (PCA) test was conducted to overcome the multidimensionality and multicollinearity issues in the data to produce valuable components. Results showed that among teaching competencies, 15 variables were analyzed through PCA...

Asma Hassan*, Zuhair Hasnain, Muhmmad Asadullah, Syed Saqlain Hussain and Muhammad Abbas Anees

...-1 and seed priming techniques water soaking, K2HPO4, K2SO4, and CaSO4 (2%) seeds for half-day used to find out effects on yield of quinoa. The maximum plant height (66.133cm) and the number of branches (5.667) were with N 95 kg ha-1. Leaf area plant-1 from 0 to N85 kg ha-1 was increased gradually but later on, decreased. Gradually the Dry matter formation increase with an increase in N level, but above 85 kg ha-1 dry matter formation was regressively declined...

Abhishek Pandit1, Suman Poudel2, Manish Gautam3* and Shambhu Shah4

...e drawbacks of this technique. Successful estrus synchronization necessitates optimum nutrition, a good body condition score, the best semen quality, general health, and an efficient estrus detection technique.

...

Hossam Mahrous Ebeid1, Ahmed Abdelkader Aboamer1*, Amgad Ahmed Abu Elella2, Ibrahim Mohamed Khattab3, Osama Hefny Matloup1, Fatma Ibrahim Hadhoud1  

...DMSC using in sacco technique. Fifteen lactating Damascus goats (averaging 45.4± 0.5 kg, body weight and averaging milk yield 900±65 g/head/day) were randomly assigned to one of three treatments for 6 weeks to study DMSC’s effect on blood metabolites and quality and quantity of the produced milk. Treatment groups were provided either a control diet or control diet with 40 g/head/day (DMSC40) or 80 g/head/day (DMSC80) of DMSC. DMSC was high ...

Shabana Ehsan1*, Aneela Riaz2, Muhammad Amjad Qureshi1, Abid Ali1, Ifra Saleem3, Muhammad Aftab3, Khalid Mehmood4, Fakhar Mujeeb1, Muhammad Asif Ali1, Hina Javed1, Fraza Ijaz1, Anwar-ul-Haq5, Khaliq-ur-Rehman3 and M. Usman Saleem

...ve biofortification technique. The current study includes the collection of rhizobacterial isolates from wheat, maize, sorghum, millet, and maize rhizosphere soil of Rawalpindi and Sargodha divisions. The screening of bacterial isolates for siderophore production through CAS-shuttle assay (quantitative) and CAS-agar (qualitative) was done. Isolates were further characterized for Fe and phosphorus solubilization, indole acidic acid (IAA) equivalents, and organi...

Khalid Khan1*, Mahmood-ul-Hassan2, Ihsan Ullah1, Haris Khurshid1, F.Y Saleem Marwat1, Salman Saleem1, M. Jehanzeb1 and Zubair Ahmad1

...fferent statistical techniques. Total 14 sorghum hybrids including standard Check, were used and data was collected on morphological traits. Cluster analysis, based on morphological traits revealed five major groups. Maximum cluster distance was observed in group I and V, while minimum cluster distance was observed in group IV and V. Highest emergence (33.7 m-2) was noticed for ICSA220XICSR90, highest leaves per plant (11.68) were reported of hybrid combinatio...

Syed Ishfaq Ali Shah1,3, Azaz Ahmad2 and Wanzhi Cai1,*

... context, Acanthaspis rafiqi Shah and Cai sp. nov., is described, including descriptions of genitalia and detailed illustrations giving important diagnostic characters. Interestingly, this species has relatively similar body size and color patterns including the structure of male pygophore and parameres, however, differs from A. quinquespinosa in the structure of the phallus.

...
Azra Kalhoro1, Abdul Aziz Mirani2, Fozia Khan Siyal1, Tahira Jatt2*, Abdul Razak Mahar1, Sadia Iram3 and Muhammad Abbas Bhutto4
...e atomic absorption techniques revealed that among the five treatments, accumulation of the six metals was found higher with 100% SW, which was decreased with decrease in SW concentration up to 25% SW. The minimum accumulation of the metal was noted with 100% FW (control). Among the five types of vegetables, cabbage and cauliflower revealed a high tendency of accumulating the metals. Hence, in order to avoid exposure of excess heavy metals to human health thro...

Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2 

...tine paraffin block technique. The sections of infected livers that were embedded in paraffin underwent immunohistochemistry. Several immunolabeled liver sections with anti-CD3, CD4, CD8, CD20, CD68, and caspase-3 were examined under a light microscope. The histological alterations caused by the hydatid cyst were cell degeneration, necrosis, hemorrhage, congestion, and fibrosis. The findings revealed that the CD3 T cells were the most predominant inflammatory ...
Rafiq Ali Khan1, Sajjad Ahmed1, Salim Raza2* and Shehla Gul1
...s and extrapolation techniques, a benchmark value for fracture gradient of Lockhart Formation is derived that is 0.91 psi/ft. This value is very important parameter in drilling operations and can be used in proper designing of drilling fluid to avoid losses and subsequent kicks/blow out for safe and successful drilling of wells. The Lockhart Formation has reservoir potential in the stratigraphic units lying on the southern side of the Main Boundary Thrust (MBT...

Marat Kuibagarov1, Assylbek Zhylkibayev1, Dinara Kamalova1, Anara Ryskeldina1, Nurdina Yerzhanova1, Yerlan Ramankulov1, Alexandr Shevtsov1*, John A Angelos2  

...e studied isolates had unique amino acid sequences that were assigned to new M. bovoculi PilA groups designated K (2 isolates), L (2 isolates), and M (1 isolate).

Keywords | Moraxella bovoculi, infectious bovine keratoconjunctivitis, pilin, pilA, PilA 

...

Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana  

...hain reaction (PCR) technique. Then the amplified products were sequenced. The results showed that the population of Pesisir cattle used in this study was polymorphic. There were 5 polymorphisms in the exon 9 and intron 9 regions, which were located at the 18 A>G, 213 T>C, +49 A>T, +232 A>C, and +252 G>A. There were 3 transition type mutations (at positions 8, 213 and +252) and 2 transversion type mutations (at positions +49 and +232). Based on ...

Lobna M.A. Salem, Nashwa O. Khalifa, Marwa O. Abd El-Halim* 

...ional morphological techniques to differentiate the two species can be inaccurate, especially when hybrid forms are present. The definitive identification of Fasciola species, including their hybrids, has been made possible by advanced molecular techniques. Our study aimed to estimate the prevalence of Fascioliasis and identify the phenotypic features of Fasciola that infecting sheep, cattle, buffaloes, goats, and camels in ...
Farzana Bashir2, Aysha Saleem1, Khalid Iqbal Khichi2, Rubina Nelofer3* and Rauf Ahmad Khan2
...M, EMAX and ICP-OES techniques. The antibacterial activity was determined by column studies using response surface methodology (RSM) based on three levels, three factorial Box Behnken Design (BBD). The significance of independent variables (flow rate, bacterial count and column height) and their interactions were tested by means the analysis of variance (ANOVA) with 95% confidence limits. The antibacterial susceptibility was optimized for flow rate, bacterial ...

Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1 

...oassays, Diagnostic Techniques, Chronic Mastitis 

...

Syeda Batool Zehra1, Abdullah G. Arijo2, Aly Khan3, Nasira Khatoon1* and Samina Waheed1

...ional morphological techniques used with, molecular biology technologies it has proven to be effective in distinguishing closely related species. Statistical analysis is used to determine the intensity of infection in relation to age and gender. This study will focus on children who are suspected of being afflicted with pinworms. The presence of adult worms or ova in the feces is required to make proper diagnosis. The swabbing of the perianal region is utilize...

Ahmed H. Abed1, Ahmed Orabi2*, Wafaa Hassan3, , Enas Hammad4 , Ghada Gaber5 

... essential oils have a unique immune-potentiating effect on broiler chickens’ immunity challenged with E. coli. One hundred and twenty broiler chicks were separated into eight equal groups in an in vitro experiment (15 birds for each group). A negative control group was the first group. There were 3 groupings were given immune modulatory agents (lectins, probiotic, and essential oil) for five days without being challenged; Immune modulatory agents were g...

Adefunke Fadilat O. Ayinde1*, Peter Allison Johnston2, Olanrewaju Olusoji Olujimi3, Purnamita  Dasgupta4 and Dare  Akerele5

...ulti-stage sampling technique. Farmers perceived climate variability as unpredictable weather situation over the years (65.41%), though some (23.31%) perceived it as the act of God or the wrath of God (11.28%). In terms of adaptation measures, 63.4% of the respondents had access to the weather forecast, some (55.4%) utilise it, while 58.4% engaged in artisanship (blue-collar jobs) and vegetable production (63.2%). Lessons on adaptation are critical for putting...

Irshad Ahmad1,2*

...sh health but also its unique array of bioactive compounds can improve coloration and quality of fillet. The aim of this review is to provide an update of the current knowledge of microalgae as a supplement or feed additive to substitute FM and FO in aquafeeds. This review will provide a platform to highlight the potential of microalgae-based aquafeeds for a sustainable aquaculture industry.

...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
...t 4°C and kept over liquid nitrogen vapors for 10 min. Then straws were plunged into liquid nitrogen (-196°C) for storage. After 24 h of storage, straws were thawed at 37°C for 30 seconds and assessed for sperm motility (%), plasma membrane integrity (%), viability (%), normal apical ridge (%) and chromatin integrity (%). Sperm motility was recorded higher in extender containing 15mM alanine compared to control. ...

Ayma Aftab, Samia Afzal* and Muhammad Idrees

... a highly sensitive technique that has also been used in detection of corona virus. This case study emphasizes the importance of HRCT for early and confirm diagnosis of COVID-19 whereas RT-PCR results can vary. This process may show negative results and is time consuming.

...

Iqra Qazi1, Sohail Raza1*, Masood Rabbani1 and Muhammad Avais2

...convenient sampling technique. A questionnaire having variables related to animal species, age, herd size, parity, breeding method and housing management was filled at sample collection time. The serum samples were processed by indirect ELISA to detect antibodies against BoHV-1. The overall sero-prevalence was 68.40%. Post pubertal animals (>3yrs) contributing more to disease occurrence. High sero-positivity was observed in buffalo (73.12%) as compared to c...

Anum Syyam1,2, Nazish Saqlain1, Sidra Hareem1, Naghmana Mazhar1, Liaqat Ali3 and Samia Afzal2*

...g the standard tube technique and the samples positive for hemolysis were further evaluated for titers of IgG type anti-A and anti-B. All the titers were confirmed microscopically. The total prevalence of IgG anti-A and anti-B hemolysins was 15.9%. The most frequently observed IgM anti-A and anti-B titers were 64 in 29.4% (n = 103) and 32 in 24.9% (n = 87) donor samples, respectively. IgG type anti-A and anti-B with hemolytic activity were present in 10.8% and...

Andy1,4*, Ratmawati Malaka2, Sri Purwanti3, Hikmah M Ali2, Tutik Lusyta Aulyani4, Danung Nur Adli5 

...ion of the encapsulated liquid smoke on growth performance, intestinal profile, and microbial profile of broiler chickens. A total 100 broiler chickens were reared for five weeks on the experimental diets. A general linear models with five treatments and four replications was employed. The treatments consisted of T0 = control, T1 = basal diet + acidal, T2 = basal diet + 0.5% encapsulation of liquid smoke, T3 = basal diet + 1...

Neni Widaningsih1,2*, Budi Hartono2, Hari Dwi Utami2, Eni Siti Rohaeni3 

...accidental sampling techniques with the main respondents, namely breeders, collecting merchants, wholesalers, retailers, and consumers. The number of respondents in this study was 135 persons. Methods of data analysis carried out include; qualitative analysis and quantitative analysis. Qualitative analysis is intended to find out the characteristics of marketing institutions, and the marketing channels of buffalo cattle. While quantitative analysis is intended...

Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*

...ffect of post pelleting liquid phytase supplementation was evaluated in broilers on the basis of growth production, plasma and tibia phosphorus. The (n=504) day-old broiler chickens procured randomly allocated into 6 treatments dietary treatments were: B positive control (PC) diet (0.42% available phosphorus in grower feed, A negative control (NC) diet with (0.30% avail. P in starter and 0.26% avail. phosphorus in grower) while four diets C, D supplemented wit...

Emtiaz Ibrahim Ghoniem, Gaber Mamdouh Abdelgalil, Amira Farouk Gad* and El-Sayed Hassan Eshra

 
...topical application technique were estimated. Additionally, in vivo evaluation of acetylcholinesterase (AChE), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in T. pisana intoxicated with two sublethal doses of CuSO4 (0.25 and 0.5 of LD50) after 24, 48 and 72 h were examined. The results indicated that the LD50 values of CuSO4 were 166.5, 92.59 and 70.63 µg/g b.w for 24, 48 and 72 h, respectivel...

Muhammad Nadeem1, Muhammad Rizwan2,3*, Tanveer Ahmad4, Muhammad Kashif5, Aneela Zameer Durrani2, Muhammad Ali6, Asghar Abbas7, Safder Imran8, Sidra Saher9, Syeda Fakhra Waheed10

... to be a worth full technique to obtain trustworthy data that may be helpful in making most effective policies for control and eradication of livestock diseases in and Pakistan. 
 
Novelty Statement | Participatory surveillance for infectious and infectious diseases of livestock is effective for diseases eradication program in Pakistan.
...

Md. Aminul Islam1*, SM Mostafizur Rahaman Sumon1, ANM Aminoor Rahman2, Fahima Khatun3 

...ll’s Dilution Technique using a McMaster counting chamber. A total of 85 faecal samples from five different dairy farms were examined. The overall infection rate of helminths in dairy cows is 37.65%. A total of five different types of nematodes were observed in all the investigated dairy farms. The highest infection rate (11.76%) and the lowest infection rate (4.71%) was found for Neoascaris spp. (Toxocara spp.) and Fasciola spp., respectively. Hemonchus...

Umair Faheem1*, Madiha Mobeen Khan2, Qurban Ali3, Muhammad Jamil4, Wajiha Anum2, Mashal Rehman2, Imran Akhtar2, Mussurrat Hussain1, Qaisar Abbas1, Ghayour Ahmad5 and Abrar Ahmad6

...eight (cm), number of silique per plant, yield loss in unsprayed blocks was compared to the yield of pesticide sprayed blocks. The yield components and yield in the insecticide free plots were much lower than that of insecticide sprayed plots which were kept aphid free. Maximum percentage reduction (14%) in plant height was observed on BRJ-9072, the silique per plant loss percentage was maximum on KP-raya (36.97%), whereas m...

Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5 

...ng the high-performance liquid chromatography (HPLC) technique. Skin histopathology and immunohistochemistry of collagen were performed. Results: Ivermectin was detected in 36 samples, out of them 15 contained high ivermectin levels (100 ppb). Chlorpyrifos, piperonyl butoxide, and acetamiprid were below the limit of quantification in 3 samples only. Histopathology of tick-infested skin revealed severe multifocal eosinophilic...

Mochamad Rizal Umami1, Zainuri*2, Sebastiana Viphindrartin2 and Rafael Purtomo Somaji2

...multistage sampling technique. The data processing includes data reduction, data display using Decision Explorer version 3.3 software. Data were analyzed through descriptive methods and content analysis. The study found that the COVID-19 pandemic formed a new pattern of sugarcane trading system, switching from a contractual trade model to a free market model (redeemer). The model refutes the theory stating that the contractual model will be more efficient than...

Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*

...ological laboratory techniques (Total plate count, Gram staining, Hydrogen sulphide, Citrate, Urease and Indole Tests). A high level of total plate count (TPC) and E. coli was observed from both raw and pasteurized milk. The results also indicated a high incidence of ETEC i.e. 63.63% and 50% in raw and pasteurized milk, respectively. The most occurring enterotoxins are ST-I and ST-II (40%) followed by LT-II and ST-II, 20% each, followed by ST-I (13.33%) and LT...

Talat Farid Ahmed1*, Muhammad Azeem Afzal1, Hashim Nisar Hashmi2, Hafiz Muhammad Yousuf2, Shamim-ul-Sibtain Shah1 and Muhammad Atiqullah Khan1 

Farah Abid1,2, Mohammad Saleem1,3*, Tahir Maqbool4, Tania Ahmed Shakoori5, Faheem Hadi4, Tahir Muhammad4, Saira Aftab4, Yasir Hassan7 and Shabana Akhtar4,6*
...itis. Real-Time PCR technique was applied to analyze the expression levels of different cytokines and interleukins involved in inflammation. Real-Time PCR results showed a down-regulation of TNF-α, COX-2, IL-6, IL-1β, NF-κβ1and up-regulation of IL-4, IL-10 and I-κB genes. Levels of antioxidants, superoxide dismutase and catalase were determined to be significantly higher (p<0.05) in the group treated with E-AM compared to the art...

Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2

... using High-Performance Liquid Chromatography (HPLC) and sugar contents were increased in acid pretreated samples.

...

Faisal Khurshid1*, Asad Ullah1 and Sana Manan2

...ied random sampling technique. A sample size of 322 farmers was selected randomly from three villages namely Palosai, Badizai and Shahi Bala of the District Peshawar, Pakistan. The conceptual framework of the study comprised of an independent variable (knowledge of innovations) and dependent variable (adoption of agricultural innovations). Chi-square test was applied to ascertain the association among the study variables. It was found that adoption of agricult...

Muhammad Azam1*, Shaista Naz1*, Malik Muhammad Shafi2, Muhammad Jamil Afridi3 and Ahmad Waheed4

...he main-statistical techniques for data analysis. It has been found that majority of the farm households (65%) were food secure, still the issue of food insecurity prevailed among the 35% of the farm households. The positive determinants included family type, monthly income, and small ruminants, while tenancy and household size were found as the negative determinants of food security. The issue of food insecurity has been coped through the adoption of various ...
Wasim Akram1, Azhar Hussain2*, Sartaj Ali2, Iqbal Hussain3 and Muhammad2
...tatistical analytic techniques were used. Using an inverse distance weight with a spherical semivariogram model, the spatial distribution maps were created. Both species had nugget/sill ratios of 0.00, 0.69, 0.61, and 0.73, indicating moderate to strong spatial dependence. The semivariogram graph also displayed moderate to high spatial dependence. The east-west and south-north trend lines of both fruit fly species crossed through the projected area of the samp...

Salma Bessalah1, Samira Jebahi2, Asim Faraz3*, Amel Raoufi2, Cem Tırınk4, Walid Dridi2, Mahmoud Daouad2, Hassib Keskes5, Touhami Khorchani1, Khaled Farah2, Abdul Waheed3 and Mohamed Hammadi1

...by physico-chemical techniques such as electron paramagnetic resonance (EPR), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) for the first time. Results revealed that gelatin yield increased significantly (P<0.05) as the extraction temperature and time increased (19.71%) at 70°C for 12 h. EPR spectroscopy demonstrated that 10 kGy of radiation-induced the decomposition of side groups. New peak positions (Paramagnetic centers) ...

Hanaa H.A. Gomaa1*, Shimaa A.S. Hasan1, Nashwa Harb1, Amal H.A. Gomaa2 and Maha Anani3

...n reaction (RT-PCR) techniques. Our findings revealed a very high frequency of HSV-1 and 2 infections among the studied pregnant women with percentages of 74.5% and 98.9%, respectively. Upon the interpretation of the HSV serological profiles, the past latent infection with HSV-1 and 2 were the most prevalent types of infection representing 74.5% and 92.5%, respectively, followed by HSV-2 recurrent infection which was more prevalent (6.4%) than HSV-1 recurrent ...

Monu Karki, Amit Kumar and Gnanavel Venkatesan*

...ORFV possesses several unique strategies, to fight against host immune environment. Some of the genes have been acquired from the host and some are flowing through the family and genus evolving with the virus to adapt and efficiently set up an infection in immune-loaded host cells. This review focuses on the important virulence genes of the orf virus and their functions with recent advances and the way they can be manipulated for different benefits to the rese...

Oluwatoyin Adenike Fabiyi1*, Mariam Temitope Baker2 and Gabriel Ademola Olatunji2

...hetics. A promising technique is the application of bio-pesticides. Organic fatty acid esters (FAE) are reassuring materials with nematicidal activities. Medicinal plants are rich source of acid esters, hence Alstonia boonei (Apocynaceae) leaves were extracted cold in ethyl acetate. This yielded crude extract that was subjected to column chromatography (silica gel 100-120 mesh grade), which afforded fractions that were analysed with GCMS and FTIR for constitue...

S. Samina and Y.I. Erum†

... richardsoni Grewal, Siddiqi & Atkey, 1992, Aphelenchoides unisexus Jain & Singh,1984, Filenchus microdorus Chawla, Prasad, Khan & Nand, 1969, Helicotylenchus urobelus Anderson,1978, Hemicriconemoides brachyurus (Loos,1949) Chitwood & Birchfield, 1957 and Irantylenchus clavidorus Kheiri, 1972 were reported. All these species were re-described with their measurements as well as illustrations. In addition, 17 new host records were reported viz., ...

M. Israr1†, M. Habib1 and K. Nasira2

...y modified Baermann technique (Baermann, 1917). After processing, the nematode genera and species were identified (Siddiqi, 2000). Tylenchorhynchus usmanensis is reported from the first time from Pakistan is briefly redescribed and illustrated.

...

M. Israr1, F. Shahina2† and K. Nasira2

...e first record of A. siddiqii Fortuner, 1970 from around the roots of carrot (Daucus carota L.), from Hasan Abdal, Punjab, Pakistan. Morphometric data of a known species A. bicaudatus (Imamura, 1931) Filipjev & Schuurmans Stekhoven, 1941 is also given.

...

S. Aatika, K. Nasira and F. Shahina†

...lenchus gulabi Jain, Siddiqui & Aruna
Parihar, 2000, Helicotylenchus jasminii Jain Saddiqui & Aruna Parihar, 2000, Pratylenchus goodeyi Sher & Allen,
1953 and Telotylenchus indicus Siddiqi, 1960 and two new host records of maize viz., Tylenchorhynchus tritici
Golden, Maqbool & Hando, 1987 and Malenchus labiatus Maqbool & Shahina, 1985...
K. Nasira, S. Shamim and F. Shahina†
...4 and Metoncholaimus siddiqii Shahina, Nasira & Shamim, 2015
while six species were reported as new records of Pakistan. These nematode species belong to 36 genera, 27 families
and seven orders. Frequency of occurrence and distribution of nematode species varied from site to site. The overall
occurrence (%) of fresh water nematode samples ranged from 3.22-32.25% at different sites. The highest number of
posit...

K. A. Tabassum, F. Shahina†, K. Nasira and Y. I. Erum

...separate lines and had unique ribosomal DNA-ITS, sequence. A compendium of the genus Oscheius
(of both insectivora and dolichura groups) based on the following characters: body length, tail, spicules and
gubernaculum length, ratios a, b, c, c' and vulva percentage is given. The morphometric and allometric characters
with anterior and posterior regions were derived from the original descriptions. An identification key to 42 v...

Y. I. Erum and F. Shahina†

...
(RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum
percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and
molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the
rest of the genotypes studied and the most effective loci to scree...

S. Anwar1, K. Wieczorek1 and E. Inselsbacher2

...ed on Ultra Performance Liquid Chromatography (UPLCTM) was performed. The results showed significant increase in arginine, proline and glutamine amounts in syncytia. Ammonium ions were also increased as an important precursor in arginine, proline and glutamine biosynthesis. In addition, branched chain amino acids and amino acids of shikimic acid based pathway were also enriched in syncytia. This study opens a door to understand the remodeling of amino acid met...

Uzma Ishaque1, Nasira Kazi1, Erum Iqbal1* and Shahnaz Dawar2

... Discolaimus conicus Siddiqi, 2005 from the roots of Saccharum officinarum L. in Mirpurkhas, Sindh, Discolaimus laksi Khan and Laha, 1982 from soil around the roots of Gossypium hirsutum L. in Sargodha, Punjab, Discolaimus omanensis Siddiqi, 2005 from roots of Mangifera indica L., in Faisalabad, Punjab, Discolaimus paratenax Siddiqi, 2005 from decaying matter in Peshawar, KP, Dorylaimoides...

Z. Gill and K. Firoza†

...nothus Allen, (1955) Siddiqi,
1980, is reported as a new record nematode species in Pakistan. It is briefly redescribed and illustrated herein.
...

Jax Vincent Gamulo, Maye Pearl Bolina, Jessica Serena Brion, Via Crishiela Nicole Dela Rosa, Roxanne Francesca Maglaya, Carl Lexter Tan, Aimee Caye Chang* 

...arts and extraction techniques used affected the resulting fasciolocidal activity of the plant extracts. Linear regression revealed a low inverse relationship between the plant extract concentration and the mortality time (R2 = 0.37). Meta-analysis revealed a significant summary effect among included studies, with high statistical heterogeneity (P < 0.05). Moreover, the forest plot showed significant difference (P < 0.05) between the activity of plant ex...
Ghulam Jelani1, Qudratullah Kalwar1*, Asmatullah Kaka2, Abdullah Channo2, Ayyaz Ahmed1, Majid Hussain Soomro1, Hidayatullah Soomro1, Deepesh Kumar Bhuptani1 and Yar Muhammad Jalbani1
...e embryo production technique, transfer of embryo from donor to recipient and the factor that are necessary for transfer of embryo from donor to recipient for production of offspring. Previously limited information existing regarding embryo transfer and its significance in farm animals therefore, in future this review might be helpful in improving the reproductive potential of farm animals.

...

Mohsin Shad1, Muhammad Usman2 and Qurratulann Afza Gardner1*

...ns in bc1 complex from ubiquinone to cytochrome c reductase in the mitochondrial respiratory chain, and similarly in b6f enzyme complex, from plastoquinol (QH2) to plastocyanin (Pc) within the thylakoid membrane. Mitochondrial cyt b in bc1 complexes contains eight transmembrane helices A to H; two of these, B and E along with two histidines, are cross-linked with two hemes located at the top and bottom of the membrane. The b6f complex contains four large subun...

Ahmad Abrar Khan1*, Ghulam Qadar1, Asif Ali Abro2 and Muhammad Awais3

...nd physical control techniques. Selection of right time, control methods, appropriate insecticides/pesticides along-with recommended dose and treatments must be defined by the recommendations of agriculture experts instead of fellow farmers and agrovet sellers. Trainings, workshops and field activities should be organized regarding improved farm management practices especially on the aspect of insects/pests management to minimize the yield losses. 

...

Saima Mumtaz1,3*, M. Imran Kasana1, Riaz Alam1, Noorullah Khan1, Muhammad Noman1, Sanjeela Sabahat1 and Hussain Shah2

...sues an alternative technique has been employed for quick multiplication of litchi. In the present study we have investigated the influence of various levels of Indole Butyric Acid (IBA) on four Litchi cultivars (Gola, Surahi, China and Bedana) for root induction, its development and success rate of survival in hardwood cuttings. Six treatments of IBA (1000, 2000, 3000, 4000, 5000, 6000ppm) and one with control (without IBA) were applied at base of hardwood cu...

Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4 

...s, and cryopreserved in liquid nitrogen for 24 hours. Consequently, straws were thawed at 37C0 for 30 seconds and motility was accessed with the help of computer-assisted semen analyzer (CASA) and membrane integrity with the hypo-osmotic swelling test. Meanwhile morphology, viability and acrosome integrity with contrast-phase microscope (eosin-nigrosin staining) was investigated. Moreover, superoxide dismutase (SOD) assay, a fatty acid composition with gas c...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
...ive technologies or techniques (ARTs) have advanced greatly during the past couple of decades. The ARTs include several techniques including artificial insemination, in vitro embryo production, oestrous synchronization, superovulation, embryo transfer, germ cells or embryos’ micromanipulation, sperm sexing, cryopreservation of ovarian tissues and germ cells and embryos, and genome resource banking for mammalian species...
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...ed expert-dependent techniques. The current work’s main objective is to evaluate the reliability of two widely distributed rapid kits for as a suitable substitution for the gold standard bovine tuberculosis diagnostic techniques. IQRT Anigen Rapid Bovine TB Ab kit and Ubio quick VET Bovine Tuberculosis Antibody kit were used in testing 3750 dairy c...

Aamir Mushtaq1*, Fatima Habib2, Umar Farooq Gohar3, Abdul Malik4 and Mobasher Ahmad2

... by cold extraction technique. Acute toxicity studies were performed in female albino mice by administration of single oral doses (4, 5, 6, 7 and 8 g/Kg/po) respectively, to individual groups (n=5) and after that the behavioral changes were observed. To find the chronic toxicity, the animals were divided in three groups (n=6) which were orally administered with normal saline, P. anisum methanolic extract 400 mg/Kg, and 800 mg/Kg respectively for three months. ...

Khasan Khamidovich Shakhbiev1*, Saidkhamzat Saidmagomedovich Abumuslimov1, Larisa. Vakhaevna Taramova1, Islam Khasanovich Shakhbiev1, Svetlana Aleksandrovna Shemyakova2 

...including of 1 g per praziquantel 250 mg, azithromycin 150 mg, fenbendazole 250 mg, copper chelate 100 mg, cobalt chloride 100 mg and zeolite 150 mg, at a dose of 20 mg / kg dog body weight which the is a biologically safe, effective therapeutic and prophylactic agent for echinococcosis of young of dogs complicated by streptococcosis. For the first time, results have been obtained confirming that the components of azithroprazifene (praz

Saba Iqbal1, Salman Khurshid1*, Hafiza Mehwish Iqbal1, Qurrat-Ul-Ain Akbar1, Aqeel Ahmed Siddique3, Saqib Arif1, Shahid Yousaf2, Masooma Munir4, Abdul Karim Khan4, Shazia Arif5,  Abdul Ahad6 and Muhammad Arif7

...he NIR spectroscopy technique showed better performance for grains possessing medium hardness. Since the NIR technique is rapid, reliable and does not require expertise for operation, it has a great potential for utilizing in grain testing laboratories to support our grain industry and trade. 

...

M. A. Haq, M. Shahid and K. Mahmood

...ng perineal pattern technique (Eisenback et al., 1981), a most common and abundant species of root-knot nematode 

W. Khan, N. U. Nisa, A. Khan and S. M. H. M. Naqvi

...Swat, Pakistan. The techniques used were wet mount (WMT), sedimentation and centrifugation. A number of 171 individuals were found infected with any single species of parasite, 81 cases were found infected with double species of parasites, 21 individuals were having triple and 4 individuals were found to be infected with four species of parasites. Nematodes were the most prevalent intestinal parasites than cestodes and protozoans. The prevalence rate was: ...

B. Archana and R. Saxena

...cognita by in vitro technique. Aqueous extract of all plant roots exhibited nematicidal activity. LC50 values calculated as 1024 ppm for C. roseus, 1867 ppm for S. nigrum, 1968 ppm for A. spinosus, 3428 ppm for C. album and 962 ppm for O. sanctum. LC50 values showed that out of these extracts O. sanctum found to be the most effective. In vivo experiment was conducted to study the effect of root extract of O. sanctum on M. incognita infecting French bean. Diffe...

Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5 

...and membrane integrity. Liquid chromatography-mass spectrometry (LCMS/MS) analysis was used to assess the confirmation profile protein of seminal plasma. The conception rate from artificial insemination was used to determine the fertility rate (AI). This investigation revealed that the quality of the sperms from Bali-polled bulls and Bali-horned bulls did not substantially change after thawing (p > 0.05). However, sperm acrosome integrity was considerably (...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

SAEED AHMED*1, ZIA-UR-RAHMAN1, SALMA KHALID2, TARIQ KHAN1, ARSHAD IQBAL3
& ZAMARUD SHAH4
...membrane filtration technique and MacConkey agar for the isolation of Enterobacteriaceae. Different identification tests have been carried out by using various selective and differential media (MacConkey agar, Eosine & Methylene Blue agar) for isolation and identification of microorganisms.Water samples showed the presence of faecal contamination; especially coliform bacteria which is a risk for the health of local community. Salmonella spp. was detected i...

Nguyen Thi Kim Dong1*, Nguyen Van Thu2 , Nguyen Hoang Qui3 

...r was the digestion technique (ileal and total tract digestibility) and the second factor was the diets. Four diets included a basal diet (SBP0), which consisted of fish meal 20% (FM), broken rice 80% (BR) and the other three diets, in which the content of FM was 25.0, 50.0 and 100% of the FM level in the basal diet and was replaced by ensiled shrimp by-products corresponding the SBP1, SBP2 and SBP3 diet, respectively. Daily intakes of ether extract (EE) and ...

AISHA, MUHAMMAD ASAM RAZA* AND UMME FARWA

...erent spectroscopic techniques and subjected to biological evaluation. In vitro model was used for enzyme inhibition activity through spectrophotometer and suggested that compounds may be helpful in the medicinal field. Compound 2 exhibits more inhibition against BChE (67.7%) while compound 1 showed maximum inhibition of α-glucosidase (65.1%). Theoretical study i.e. docking of the prepared compounds was also related to experimental work. In-Silico ADMET ...

AZKA ASIF, HAREEM MOHSIN*, SADIA N CHAUDHRY, & WARDA FATIMA

...uding oil spreading technique (OST), tilted glass slide assay (TGS), drop collapse assay, emulsification index E24 and emulsification assay were used to evaluate production of biosurfactants in five biochemically identified strains isolated form oil contaminated sites. All strains used were known producers of biosurfactants but showed variable results for different tests. Four of five strains produced similar results for OST, TGS and emulsification assay. Drop...

HIRA JAVAID*1, MALEEHA MANZOOR2, JAVED IQBAL QAZI3, XIONG XIAOCHAO4 & FOUZIA TABSSUM5

...omic crisis. Of all the liquid biofuels, an important one is biodiesel, the fatty acids methyl esters. Due to beneficial decomposition of bio-waste resources towards clear environments, oils from single cell microbes (yeasts) are gaining attention. Further cost reliance of these microbes to produce single cell oils (SCOs) could be reduced by using lignocellulosic waste (LCW) biomass produced in millions of metric tons annually all over the world covering 60% o...

Zubair Ali, Muhammad Sohail*, Yasir Ameen, Hamidullah, Ishtiaq Ahmed and Mehwish Malik

...onography is modern technique being utilized for management of reproductive efficiency of dairy cows all over the world. The ultrasound facility was not available in the study area due to which farmers were complaining about reproductive issues of their animals. Therefore, this study was conducted for assessment of reproductive health of cow genital tract using real time B mode Ultrasonography in order to improve the economics of the farmer through decreasing ...
Jian-Cheng Zhai1,2, Sheng-Nan Wang1,3, Qiang-Hui Wang1, Yan-Ling Xia1, Wei-Shi Liu1, Ya-Jie Yin4 and He-Ping Li1*
...ate for regulating the unique growth of female reindeer antler.

...

Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3

...people worldwide. This unique RNA virus escapes the immunity in most infected people and forms a chronic infection which can progress to liver cirrhosis, fibrosis, hepatic cancer, and death. The goal of this work was to assess the cytotoxicity and in vitro inhibitory activities of local medicinal plant Azadirachta indica (A. indica) extracts on expression of NS3 and NS5A nonstructural proteins of HCV 3a genotype. Owing to the fact that HCV patients can develop...

Hemayet Hossain1, Md. Masud Parvej1*, Khadiza Akter Brishty3, Muhammad Ali4, Junayed Ahmed4, Asibul Hasan4, Rubel Miah4, Md. Piplu Mia4, Saad Muhammad Rafe-Ush-Shan4, Md. Nazmul Alam4 and Md. Zahidul Islam Khan2

...g common laboratory techniques. A total of 28 infectious and non-infectious diseases and disorders in ruminants were detected, whereas the pooled prevalence (PP) of the infectious disease group in cattle was 38.44%, (95% CI: 33-42) and in goats it was 34.43%, (95% CI: 29-39). Among them, FMD was recorded the highest prevalence at 5.78%, (95% CI: 3.8–8.4) and the lowest prevalence at 0.89%, (95% CI: 0.2-2.1) for Tetanus in cattle. The PP of metabolic dise...

Ovirup Bhushan Paul1, Shodipta Sharma Urmi2, Md. Ashraf Ali Biswas3* 

...ants. Novel feeding techniques were implemented that combined fermentation to improve digestibility with locally accessible feed supplies using a completely randomized design. TMR feed was produced with 70% roughage of silage & rice straw and 30% concentrate mixture. FTMR feed was produced by mixing TMR feed with molasses containing Saccharomyces cerevisiae. Prepared TMR and FTMR feed mixed with rumen fluid buffer for in vitro digestion at 6h, 12h, 24h, &a...

Riko Noviadi, Dwi Desmiyeni Putri, Gusma Gama Maradon*, Agung Adi Candra, Nani Irwani, Gadis Apriani, Made Guntur Candra Adinata, I Made Krisnanda

... various processing techniques in broiler rations. This study used a completely randomized design (CRD) with 4 treatments and 5 replications, each replication consisting of 5 broilers. We designed four treatments, P1 = Sundried for 2 days + grinding, P2 = Oven dried at 50°C for 7 h + grinding, P3 = Roasting at 100 °C for 15 min + grinding, and P4 = Water scalding at 96 °C for 1 min + grinding. The processing techniq<...

Imran Khan1*, Shahid Amin2, Safiullah3, Shah Zeb Khan3, Abidullah3, Amjad Ali3, Saghir Imdad Hassan3 and Israr Ud Din3

...d thin blood smears techniques as per method described by (Adam et al. 1971). A higher infection rate was found in females (6.67%) as compared to males (4.67%). Highest (9.00%) infection was noted in camels above 4 years of age group followed by (5.00%) in camel under 2 years age group while lowest (3.00%) in 3-4 years age group camels. It was concluded that 5.67% (17/300) samples were found positive for Trypanosoma evansi infection. Infection rate was found h...

Anhar Ibrahim Elhanafy*, Amr Mohamed Mousa, Amaal Mohamed Kamal 

...he radioimmunoassay technique. Results suggested that BV can be an effectual and safely naturalistic immune stimulator, anti-inflammatory and antioxidant substitute to pharmaceuticals to be applied in rabbit farms. Furthermore, rabbit wellbeing was significantly influenced by injecting < 0.3 mg BV/rabbit twice weekly. 

...

Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi

...">Cryptosporidium is a ubiquitous protozoan parasite causing gastrointestinal disorders in various hosts worldwide. The current study was undertaken to study the biology of Cryptosporidium meleagridis and examine the anti-Cryptosporidial efficacy of curcumin in experimentally infected quails compared with that of paromomycin. This study carries out from September 2022 to January 2023 in Baghdad city, Iraq. Oocysts were isolated from naturally infected quails i...

I Nyoman Sulabda1, Anak Agung Gde Oka Dharmayudha2, I Wayan Nico Fajar Gunawan3, Dwi Fortuna Hashilonda4, Luh Gde Setyawati5, I Ketut Puja6*

...by the Federation Cynologique Internationale since 2019. To become a definitive breed, it takes ten years to improve its quality and population size. If this was not achieved, the Kintamani dog was removed from the breed category. Therefore, efforts must be made in the future to increase the population and genetic quality of Kintamani dogs. This study aimed to provide frozen semen of Kintamani dogs for rapid improvement of the quality and population of Kintama...
Lubna Anjum Minhas, Abdul Samad Mumtaz*, Muhammad Kaleem, Rooma Waqar and Jamila Annum 
...inardtii, Acutodesmus obliquus and Cosmarium isthmocondrum are new record from Pakistan. Among identified taxa, Scenedesmus was the dominant genus with 8 species and their contribution was 26.6%. The 2nd most dominant genus was Cosmarium with 6 species and their contribution was 20%. The other genera Chlorococcum, Ankistrodesmus, Coelastrum, and Closterium were (6.6%). Some genera represented only one (3.3%) species that were Chlamydomonas, Eudorina, Tetraspor...

Muhammad Ehsan Safdar1, Muhammad Asif1, Amjed Ali1, Ahsan Aziz1, Naeem Akhtar2, Muhammad Shahid Gulrez1 and Waqas Raza3*

...eight (195.0 cm), and silique length (6.06 cm) biological yield (15.67 tons ha-1), seed yield (3.85 t ha-1) and oil yield (1188.3 kg ha-1) of canola were recorded with 2 kg ha-1 B + 10 kg ha-1 Zn treatment. The enhancement in seed and oil yield of canola seems to be due to improvement in seeds per silique (29) and 1000-seed weight (4.25 g). A synergistic relationship might exist between these two nutrients as their combined ...

A.S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik 2; Nashwa M. A. Abd El-Mohsen1; and H. M. Abdelmaksoud1

... by gene expression technique in E. coli through: (I) Insertion of CP gene isolated by RT-PCR into Pinpoint Xa-l Vector by ligation and propagation after transformation process in E. coli. (2) Isolation of plasmid DNA. then used restriction enzymes Bam HI and Bgl II to identify clones containing inserts. To confirm the fragment inserted of CP gene sequence. PCR was performed using specific primers for PVYN CP gene. (3) The gene expression was performed using 1...

Hala A. Amin1; A. Barakat2; A.A. Abou Zeid1

... on immunoenzymatic techniques requires significant amounts of immunoreagents. Hence, using directional cloning technology, a 38-KDa fusion protein containing a complete coat protein (CP) gene or Citrus tristeza virus (CTV) encoded by the expression vector plasmid (Pinpoint™ Xa3, Promega) with a fragment of biotin binding protein (BBP-rCTV-CP) was highly expressed and purified from E. coli cell culture. Antiserum obtained from rabbits after injection wit...

A.Y. Ahmad1, M. A. Amer2, T. A. Mostafa1, F. M. Abo El-Abbas1, and M. El-Hammady1

...udied using several techniques. Many different symptotypes were appeared on Chenopodium amaranticolar. Ch quinoa. Datura metal and Nicotiana tabacum cvs. Samsun and White Barley as a result of mechanical inoculation. All isolates reacted positively with monoclonal antibody specific to Potato virus Y group N. Reverse transcription-polymerase chain reaction (RT-PCR). hemi-nested and nested-RT-PCR were used to study their molecular characters. Three pairs of prim...

Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2

...6 hrs. Local lesion technique for biological purification and electron microscopic analysis confirmed the production of mature infectious virus particles underlining the conclusion that a full infection cycle was completed in this system. Both the structural viral proteins nucleoprotein (N) and the envelope glycoproteins GI and G2 and the nonstructural viral proteins NSs and NSm were accumulated to amounts sufficient for detection and cytopathological analysis...

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

...PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysis of the extracts on 5% polyacrylamide gel. A reverse transcription-polymerase Chain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA product of approximately 360 bp was successfully hybridized with digoxigenin labeled PSTVd...

Om-Hashem M. El Banaa l, A.A. Abou Zeid2, Fawzia I. Moursy3 and Azza, G. Farag

...n Reaction (IC-PCR) technique was applied for detection of S. citri Saglio et al in which the Spiroplasma was captured with the specific polyclonal antibodies on a solid-phase. Specific primers based on the sequence of the Morocco strains of S. citri Saglio (R8A2HP and G113) were used. Two pairs of primers SC. SC’ and SC8. SC9 were used. PCR fragment of correct size 330bp was amplified with primers SC. SC expressing spiralin gene and 760bp was amplified ...

Sahar. A. Shoman1 and B. A. Othman2

...specificity of this technique. the n-PCR-expected product (869bp) was sequenced, and this nucleotide sequence was analyzed using a software program. Sequence analysis of the amplified fragment revealed a high conserved sequence homology located at the 3'-terminal region of the 126KDa (ORF1) sequence of the published TMV strains.  Detection of the TMV-E using RT-PCR raises the availability of species-specific primers for the TMV-E that will be helpful for ...

Muhammad Ismail1, Abu Bakar Muhammad Raza1,2, Zarina Qasim3, Muhammad Zeeshan Majeed1*

...ent pest management techniques, i.e. field sanitation practices, installation of methyl eugenol pheromone based male fly trapping and application of protein hydrolyzate based female fly trapping baits, for the management of fruit fly infestation in citrus orchards. Combination of these IPM strategies significantly reduced fruit infestation and enhanced fruit yield.
...

Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5

...lant tissue culture technique to develop a mass propagation system for the effective multiplication and conservation of P. minima. Juvenile leaves were cultured on MS basal medium supplemented with 0.1, 0.5, 1.0, 2.0, and 5.0 µM thidiazuron (TDZ). As a result, 100% callus induction was achieved on the medium containing 1.0 µM TDZ. Notably, calli grown on 1.0 µM TDZ exhibited greater potential for shoot regeneration and proliferation in the gr...

Muhammad Arshad Iqbal*, Aamir Ghafoor, Irfan Ahmad, Muhammad Ijaz 

...rictions. Bacillus amyloliquefaciens has been investigated as potential probiotic. The study involves the evaluation of competitive exclusion ability of B. amyloliquefaciens against S. Enteritidis and Cl. perfringens. A total of 180 commercial broiler chickens were obtained from local hatchery and were reared for 42 days. Three groups (A, B, C) of birds were divided into 3 subgroups with 20 birds in each group. Group A (-ve ...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...sed microbiological techniques, while chain reaction technique (PCR) was used for detection and characterization of Stx-1 and Stx-2 genes. In total, 75 (46.8%) isolates were detected as E. coli. Among them, 14 (8.75%) isolates were tested positive for Stx genes (Stx-1 and Stx-2). The detection of pathotype of shiga toxin producing E. coli O157:H7 in chicken meat is a significant finding because this pathogen has been related...

Kétomon Pierre Challaton1*, Coovi Guénolé Akouedegni1, Kadoéito Cyrille Boko2, Goué Géorcelin Alowanou1,3, Pascal Venant Houndonougbo4, Aboudou Habirou Kifouly1, Mawulé Sylvie Hounzangbé-Adoté1  

...ing the Mini-FLOTAC technique for the quantitative study and the Baermann method for the qualitative research. The results showed an overall prevalence of gastrointestinal parasites of 96.82 %. Furthermore, goats were mainly infected with coccidia (92.24 %); strongyles (83.91 %), of which the critical genera were Haemonchus sp., Trichostrongylus sp., and Oesophagostomum spp. ; Strongyloides spp. (73.25 %) followed by Moniezia spp. (21.8 %). Other gastrointesti...
Muhammad Fiaz1, Muhammad Farooq Hussain Faisal1
Muhammad Imran Khan1*, Kashif Ishaq1, Tanveer Ahmad1 and Qazi Jamil Akbar2
...lyzed through ANOVA technique using SAS 9.3 software. Results indicated that weight gain was higher (P<0.05) and feed conversion ratio was lower (P<0.05) in lambs fed diets with 20 and 30% peanut haulms than other two diets. Heart girth and body length were increased with increasing inclusion rate of peanut haulms from zero to 30%. It is concluded that peanut haulms might be potential and economical roughage source to be used as roughage ingredient up to...

Jihan Abd Wahab, Nur Bahiah Mohamed Haris* and Jasmin Arif Shah

...g a random sampling technique. A total of 362 extension agents from Peninsular Malaysia were participated in this research. Based on the result, all independent variables (PIME skills) indicated a positive correlation towards work performance. The monitoring and evaluating are the skills that significant towards work performance, and the evaluating skill became the highest independent variable that contributes to the work performance of extension agent. About ...

Hassan Raza1, Muhammad Kashif Afzal2, Muhammad Luqman1*, Tahir Munir Butt3, Muhammad Yaseen1 and Muhammad Umer Mehmood1

...advanced production techniques. Keeping in view the above-mentioned facts, it was imminent to assess the awareness level and adoption of olive growers of the Pothwar region of Punjab so that they can contribute effectively to the uplift of agriculture as well as their livelihoods. Pothwar region comprises Attock, Chakwal, Jhelum, and Rawalpindi districts. Three districts (Chakwal, Jhelum, and Attock) were purposively selected due to the maximum number of olive...

Seyi Olalekan Olawuyi1*, Adedotun Oluwagbenga Anjorin2, Oluwagbenga Titus Alao3, Tosin Dolapo Olawuyi3, Rachael Ajibola Ayinla3 and Rasheed Ayodele Ayinla3

...age random sampling technique. Data were analyzed using frequency distribution and percentages, cross-tabulation, and food insecurity experience-based scale for the continuum categorization of farmers’ food security status. Ordinal logistic regression model was applied to estimate the effect of adoption of bio-security practices and other dynamics on the farmers’ levels of food security status. Findings revealed that crop farmers were aware of bio-...

Nitya Salsabila, Djalal Rosyidi, Agus Susilo* 

...onication coconut shell liquid smoke at different times on the activity of water (Aw), water, fat, ash, carbohydrates by difference, and organoleptic quality (color, texture, aroma, and taste). The research material used was jerky made from duck meat with the addition of coconut shell liquid smoke and spices such as salt, coconut sugar, garlic, galangal, coriander and tamarind. The method used was a laboratory experimental m...

Muhammad Mukhtar1*, Lely Ummi Wakhidah2,Mohammad Zubair Hippy3 

.... The data analysis techniques used are Location Question (LQ) analysis, LQ Shift Share, Analytic Hierarchy Process (AHP) and SWOT (Strength, Weakness, Opportunities, Threat) analysis. The result of this study indicate that beef cattle are livestock that have regional advantages (base sector) and are a progressive sector in the development of beef cattle in Gorontalo because they can be a catalytic factor in regional economic development and meeting people&rsq...

Rukhsana Anwar1*, Muhammad Usman Khalil1 and Kanwal Ashiq1,2

...e (NIBP) monitoring technique was used to assess the blood pressure of rats. The frequency of hippuric acid synthesis from the hydrolysis of Hippuryl-L-Histidyl-L-Leucine was utilized to calculate angiotensin-converting enzyme (ACE) activity. The results showed that in comparison to hypertensive rats, the test doses of the extract significantly decreased systolic blood pressure (96.75±3.9 mmHg and 104±3.8 mmHg), diastolic blood pressure (73.12&pl...

Asim Zubair1*, Ayesha Aziz2, Ghulam Akbar Malik3, Iffat Batool4 and Zohaib Atta Mehdie5

...ing random sampling technique. The data that had been gathered, was examined using SPSS. According to the findings of our research, women in the study area were energetically involved in agricultural activities. To better meet the requirements of their family, they invested more time in agricultural activities.

...

Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3

...traw, and refrozen into liquid nitrogen vapor about 5 cm above the liquid nitrogen surface for 10 minutes. Freezing was run by immersing the forage in liquid nitrogen for 24 hours before the post-thawing examination. Results showed that spermatozoa diluted with egg yolk citrate supplemented with 3% lycopene (P3) had better quality than that found in the other four treatments, namely motili...

Ying Liu1,2, Ji-Yong Fang2, Na Zheng3 and Hai-Long Wu1*

...dica and exhibit a few unique characteristics, including more denticles in the lip, shorter intestinal caecum, longer spicular, more caudal papillae, and pre-, ad-, and post-cloacal caudal papillae pairs in the ratio of 3: 1: 6-7. BLAST analyses of the COI sequences show 59.31% nucleotide divergence with Seuratascaris numidica (Seurat, 1917) (GenBank acc. no. MG434691 and MG434692). Through morphological and molecular characterization of S. schmackeri sp. nov....

Yajuan Zheng, Qiuping Mo, Hongchao Tang, Qinghui Zheng and Dandan Guan*

Abdul Zahir1*, Asad Ullah1, Muhammad Jawad1 and Syed Attaullah Shah2

...ied random sampling technique from three irrigation sections in District Malakand, District Charsadda, and District Mardan, Khyber Pakhtunkhwa (KP). This study revealed that majority of the respondents did not attend formal school, cultivated 5–12.5 acres arable land (small farmers), and had moderate monthly family incomes (PRS 21000–50000). However, the respondents had the least information regarding revision of water distribution schedule, mutati...

Ahmad Noor and Malik Muhammad Shafi*

.... Boreholes pumping technique was used mostly for irrigation. The Logit model investigated the impact of climate change was responsible for 73 percent variation in groundwater level. These climate factors included precipitation (Rainfall), temperature, humidity and delayed seasons. Rain patterns such as decrease in rainfalls with low frequencies showed positively significant impact on the groundwater level as climate factor and decline in rainfall showed signi...

Skenndri Safae1*, Charrat Nadia2, Jmiai Mehdi3, Nassik Saadia1 

... panel of tools and techniques, ranging from numerical tools used to gather data from the farms to bacteriological material geared toward pathogens detection. The microscopic lesions found on most of the farms of the P group on day 35 indicated the ongoing of a regenerative process, contrarily to the farms of the D and G groups, in which the guts still underwent inflammation on day 35. Coccidiosis infection was heavily widespread on birds at day 35. Difference...

Asifa Hameed1*, Haider Karar2, Abdul Ghaffar1, Abid Hameed Khan1, Muhammad Mubashir1 and Ghulam Mustafa1

...ous globally for their unique sweet taste and are exported to the Middle East, China, Afghanistan, and different European countries. Although mangoes have been cultivated in Punjab, Pakistan since ancient times, yield of mangoes is still very low, about 8-10 tons per hectare. Successful pollination is required to achieve a bumper crop. In this regard, an experiment was conducted at Mango Research Institute, Multan to understand the role of insects’ in su...
Muhammad Rizwan1, Hamid Akbar1*, Aftab Ahmad Anjum1, Muhammad Arif Khan1, Aneela Zameer Durrani2, Muhammad Abid Hayat3, Anjum Masood4, Muhammad Talha Sajjad1 and Nadeem Raza2
...ted through Dirksen technique. Blood samples of both groups were collected on days 0, 7, 14, 21, and 28. Levels of various oxidative, metabolic and hematological parameters were measured by authenticated standard methods. We observed that serum levels of MDA and BHBA were higher at day 0 to day 14 (P<0.01), while the level of nonesterified fatty acid (NEFA) was higher at day 0 to day 7 (P<0.01) which dropped down to normal at day 21 (P>0.05) post-surg...

Muhammad Salman Ikram1, Tahir Mehmood1,2*, Sehrish Firyal3, Huma Sattar4, Shagufta Saeed3, Fareeha Nadeem3 and Muhammad Imran Mahmood Khan5

...s review describes the uniqueness of structure and relative position of Y-STRs loci, history of Y- chromosome, role of genetic markers like Y- Short tandem repeat (Y-STR) and Y- Single nucleotide polymorphism (Y-SNP), and reveal the importance of next generation sequence (NGS) as an advanced technology in forensic genetics. This manuscript also demonstrates the unique features of Y-STR markers and their different application...

Muhammad Hasnain1*, Muhammad Hussnain Babar1, Ghulam Sarwar1, Muhammad Kashif Nadeem2, Sajid Nadeem3, Muhammad Akram4, Ali Raza4, Mussurrat Hussain5, Qaisar Abbas5 and Muhammad Shahid4

..., fenpyroximate 5% SC (Unique-M), diafenthiuron 500 SC (Trophy), hexythiazon 10% WP (Nissorun) and abamectin 1.8% EC (Commando) against brinjal mites, Tetranychus urticae, on brinjal crop under field conditions. Brinjal vegetable was raised under standard agronomic practices. Field recommended dose of these acaricides were used knapsack sprayer. Results showed a significant mite reductionby allacaricides as compared to control. Among the tested acaricides, hex...

Ramzan Ali1, Erum Iqbal1*, Muhammad Ismail Bhatti2 and Saboohi Raza1

...horhynchus mashhoodi Siddiqi and Basir, 1958, Pratylenchus curvicauda Siddiqi, Dabur and Bajaj, 1991, Pratylenchus elamini Zeidan and Geraert, 1991, Hemicyclophora punensis Darekar and Khan, 1981, Tylencholaimus shamimi Islam and Ahmed, 2021, Makatinus punctatus Heyns, 1965 and Labronema mangalorense Ahmad and Ahmad, 2002 are being presented in this paper. The morphology and morphometric traits of native populations of these...

Muhammad Ali1*, Rukhsana Perveen2, Khalil Ahmed3, Ghulam Raza1, Muhammad Akbar1, Maisoor Ahmed Nafees3 and Muhammad Ayub4

...akistan. This may be a unique contribution to the genus Rodolia with the description of this species on the basis of important diagnostic characters including male and female genitalia. It is an important predator of scale insect pest, Icerya aegyptiaca (Douglas, 1890) which is dangerous insect pest upon different fruit trees including date palm, mango, mulberry and other important fruit trees in this region. This study also investigates the predatory role bot...
Maria Azam1, Tahir Mahmood Qureshi1, Saddam Hussain2, Amjad Islam Aqib3*, Shanza Rauf Khan4, Kashif Akram1, Misbah Ijaz5, Maheen Murtaza6, Afshan Muneer6 and Sammina Mahmood7
... purposive sampling technique. Following Bergey’s manual of determinative bacteriology, S.aureus was identified and antibiotic susceptibility was tested using clinical laboratory and standard institute guidelines. On the other hand, resistant strains were put to antibacterial testing assay against metallic nanoparticles both by well diffusion and broth microdilution method. To analyze the data, both probability and non-probability statistical tools were ...
Tehreem Fatima1, Jabbar Khan1,*, Hamid Shafiq2, Dost Muhammad3, Muhammad Rafi1 and Shoaib ur Rehman1
...sing sandwich ELISA technique. The maximum serum Hsp70 level observed was 42ng/ml and minimum serum Hsp70 value was 13ng/ml, with median value of 28ng/ml. In control group, maximum concentration observed was 38ng/ml while the minimum serum level was 18ng/ml with median value of 13ng/ml. In longevity males, serum Hsp70 levels increased in individuals between 89 to 91 years of age, peaked between 92 to 97 years but comparatively lower having the age of above 98 ...

Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2* 

...are clustered within a unique lineage (Africa-4 lineage of the cosmopolitan clade), no report provided detailed description of this lineage genome and the last published whole genome dates back to 2009. In this context, a complete RABV genome (5EG-QH19) was obtained from a stray dog, analyzed, and compared to other RABV sequences to update the present knowledge. Methods: Rapid immune-chromatography and direct fluorescent antibody test (DFAT) were applied to de...

Wizna1, Rusfidra2, Robi Amizar3, Romi Andika1, Muhammad Haikal1, Zurmiati1*

...tels with Bacillus amyloliquefaciens on total colony count of Bacillus sp. and nutrition content of leftover food.   The research, a 3x3 complete randomized design factorial, consisted of two factors, and each treatment was repeated three times. Factor A is the dose of B. amyloliquefaciens (3, 4, and 5%), and factor B is the fermentation time (3, 4, and 5 days). The total colony count of Bacillus sp., water content...

Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2

...h straws, and frozen in liquid nitrogen at -196 °C. Evaluation of semen was achieved pre-freezing in equilibrated semen and post-thawing in semen thawed in a water bath at 37oC for 30 seconds. Results showed that PEE2 showed the highest (P < 0.05) positive impact on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm p...

Rida Ahmad1,2*, Zulfiqar Ali2*, Farkhanda Manzoor1, Usman Ahmad3, Safdar Sidra4, Irfan Zainab2, Muhammad Furqan2, Aliza Batool1,2 and Zaidi Zona2

...7 using point count technique. Thirty points were selected from each habitat. A total of 175 species and 24,933 individuals belonging to 16 orders and 55 families were recorded. Human settlements had the highest species richness (106) while Dry Temperate habitat had the highest value of species diversity (H’=3.71). The most abundant species were Common Myna Acridotheres tristis (RA=8.599), Carrion Crow Corvus corone (7.486), Large-billed Crow Corvus macr...

Lichun Jiang1,2*, Lan Zhu1, Meiqi Li1, Xinyue Bao1, Zhenkun Zhao2, Haifen Qin2 and Wei Chen3*

...odus cephalophus) is a unique species in the genus Elaphodus. Currently, very little is known about its nuclear and mitochondrial genomes and their phylogenetic position within the family Cervidae. In this study, we have determined the complete sequence of the mitochondrial genome of the tufted deer, by using a long polymerase chain reaction (PCR) technique. The entire mtDNA sequence is 16,196 bp in length, which compared wi...

Irtaza Hussain1, Muti ur Rehman Khan1*, Asim Aslam1, Masood Rabbani2 and Ahsan Anjum1

...assay (ELISA). This technique is sensitive and cost-effective for detecting disease in large no of animals. For this purpose, serum samples from 350 goats and 91 sheep were collected on the 20th day post-infection for detection of antibodies, and essential information related to potential risk factors was collected through a questionnaire from fourteen districts of Punjab, Pakistan. Serologically positive samples were processed through PCR for confirmation usi...

Tahir Rasool Qamar1, Sanaullah Iqbal1*, Muhammad Nasir1 and Habib-ur- Rehman2

...onding author: sanaullah.iqbal@uvas.edu.pk
...

Nasir Ali1,2,6, Muhammad Ilyas2,3, Nazia Akbar1,2*, Gohar Rahman2, Shakirullah Khan4, Mujaddad Ur Rehman6, Abdul Samad5, Habib Ahmad1,2 and Bibi Nazia Murtaza7*

...r results revealed 25% unique haplotypes in ancient samples whereas 28% unique haplotypes have been observed in the modern samples. The most frequent haplotype found was H2a (23% in ancient and 10% in modern samples). Our results show that with the exception of ancient Dir, there are genetic similarities among the ancient lines between Hazara, Chitral and Swat. It revealed that most of the ancient and modern samples belong t...

Sumaira Kousar* and Safdar Ali Shirazi

...mation System (GIS) techniques. The RUSLE model, celebrated for its simplicity and efficiency, offers a lens to assess soil losses quantitatively, categorizing the basin area into five distinct vulnerability groups predicated on their proneness to soil erosion. The study’s findings spotlight alarming terrain vulnerability, specifically in the district’s northern expanse, encompassing locales like Kalam, Bahrain, and Pashmal. Within these regions, t...

Nadeem Akmal1, Abid Hussain1*, Muhammad Yousaf2, Waqar Akhtar1 and Hassnain Shah1

...g economic analysis technique. As, Basmati-Super and Basmati- 386 are main rice varieties grown in the country. Thus, this study is based on data of 46 purposively selected farmers for the crop season 2021, including 30 and 16 farmers of Basmati-386 and Basmati-Super, respectively. Cost comparison of the crop produced through mechanical and conventional manual transplanting techniques revealed that costs of raising the nurse...

Muneer Abbas1*, Sohail Abbas2, Imran Faraz3, Niaz Hussain1, Muhammad Aslam1, Muhammad Irshad1, Mudassar Khaliq1, Abdul Ghaffar1, Zubeda Parveen1, Muhammad Nadeem1, Sana Ullah4* and Malik Akhtar Iqbal5

...ssfully adopted IPM techniques to manage crop pests. However, in countries like Pakistan, the adoption of IPM practices remains limited among farmers, resulting in a lack of significant success stories in this regard. In light of the challenges posed by pest adaptation and pesticide resistance in the context of climate change, it becomes imperative to consider IPM as a viable solution. Farmers aiming to implement successful IPM programs should prioritize under...

Muhammad Nadeem1, Jamshaid Iqbal2, Tariq Mustafa3, Gul Rehman2, Muhammad Faisal Shahzad2, Muhammad Younas4,5*, Aftab Ahmad Khan6, Ameer Hamza2, Abdul Ghaffar1 and Muneer Abbas1

...ble pest management technique. Entomopathogenic fungi (EPF) are currently used as biocontrol agents and are alternatives of synthetic insecticides in sustainable agriculture. The bio-efficacy of entomopathogenic fungi (EPF); Metarhizium anisopliae (PacerMA), Verticillium lecanii (Zimm) (Mealikil-VL), Isaria fumosorosea (Wise) and Beauveria bassiana (Bals) (Racer-BB) were investigated against the mung bean thrips, Megalurothrips distalis Karny (Thysanoptera: Th...

Momina Raheem1, Muhammad Fiaz2*, Muhammad Mushtaq1, Umana Niazi1, Evelyn Saba3 and Mansoor Abdullah3

...lyzed through ANOVA technique using SAS 9.3 Portable and Sigma Plot Software under Completely randomized design. Weight gain of ewes was increased (P<0.05) substantially throughout period with CRS 25g/d and 50g/day but difference amongst treatment was not significant. The weight of the lambs at birth and 7th day age was increased (P<0.05) with CRS 25g/d and 50g/d as compared to control but no difference (P>0.05) was found between treatments. The highe...

Syed Nouman Shah1, Anees Ur Rahman1, Abdul Kabir1,2*, Midrar Ullah3, Shahab Ahmad Nawaz1, Muhammad Said1, Abdul Hafeez Bukero1, Maaz Ahmad1 and Muhammad Sadiq1

...arvest and ensiling techniques, silage additives and preservatives, postharvest management practices, feed management practices, and mycotoxin binders. We found that proper harvest and ensiling techniques; silage additives and preservatives that prevent fungal growth; regular monitoring of storage conditions and feed samples; well-mixed feed formulations with mycotoxin binders; diversified feed ingredients and optimized feed...

Saime Betül Baygeldi1, Barıs Can Güzel1*, Ramazan Ilgün2 and Zait Ender Özkan1

...licone plastination technique to the tongue, it is aimed to compare the SEM images before and after plastination. German mast geese used as study material were obtained from goose breeders. After the micro-anatomical features of the German goose tongue were determined, SEM (FEI-Quanta; FEG 250, USA) analyzes were made and their general structures were photographed. Then, silicone plastination processes were applied at room temperature. The SEM images of the fr...

Khalid Mahmood1*, Muhammad Akbar Khan1, Sayyed Ghyour Abbas1,2, Muhammad Adeeb Babar3 and Muhammad Asim4

... recovered material is unique and rare in the Siwaliks of northern Pakistan. It also increases the stratigraphic range of this barbourofelines species from the Chinji Formation to the Nagri Formation.

...

Muhammad Faizan1, Muhammad Nasir1, Muhammad Waqar Hassan1*, Ghulam Sarwar2 and Moazzam Jamil3

... chickpea (BWP-white, Sadiq-2021 and Bittle-21) against the seed weevil Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) under laboratory conditions. Biology of beetles was assessed in the form of growth index (GI) which showed maximum GI values in cowpea and mung bean varieties and least in chick pea genotypes and because of this cowpea and mung bean genotypes were classified in susceptible category and chickpea varieties were classified in resistant cat...

Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1 and Abdur Rahman Ansari1

...ed through McMaster technique for the Eimeria was 700, while that for Capillaria was 850. Proper control measures, good management and public guidance are needed to control these infections. This is the preliminary study performed for determining the incidence of endoparasites in doves in the district Jhang. However, the incidence of these parasites in doves requires confirmation through molecular assays. 

...

Ahmed Jaafar Mousa*, Nothaila Rasheed Hamid 

...hain reaction (PCR) techniques, 30 (30%) of the 100 collected samples were found to be positive. A DNA sequence of 372 bp length that partly encompassed the coding region of the ITS1-5.8s rRNA-ITS2 gene within ten samples (assigned 1-10) was amplified in this study. The PCR amplicons that were found in the amplified genetic region were directly sequenced. The detected variations were then used to build a phylogenetic tree, which was used to evaluate the precis...

Ihab G. AL-Shemmari1*, Ali Hussein Fadhil1, Mohammed Assad S. Alkabi1, Eman Jawad Jabber2 

...n of molecular techniques and culture yielded the highest reliable results, suggesting that this method might be employed as a rapid regular screening test. 

...
KHALID JAVED IQBAL1, ARSHAD JAVID2, NOOR KHAN3, IRFAN BABOO4, AHMED ALI5, KASHAF
MOHSIN6, AZRA ANWER7, USMAN ATIQUE8, MUHAMMAD ALTAF9, DILAWAR HUSSAIN10 & ASMA
CHAUDHRY11
...rvey data, analysis techniques were carried out to achieve attitude
measurement for a set of scales. Significant percentage effects were
found with age, profession and education of fish consumers. Percentage
of those fish consumers found to be higher which were with age of <25
(43%), master passed (40%), students (49%). Quality, quantity and
effectiveness of fish consumption were also found to caus...
FARZANA SIDDIQUE*1, NAZIMA FIRDOSE2, MUHAMMAD ARSHAD2, WARDAH HASSAN2
& SHAFAAT YAR KHAN2
...sticides using HPLC technique. Results showed that 100 % of milk
samples were contaminated with pesticide residues. Maximum
concentration of malathion (0.72±0.02 μg/kg) and deltamethrin (0.75±0.04
μg/kg) was found in cow milk samples collected from Sargodha. Minimum
pesticide residues were found in cow milk collected from Tehsil Bhera.
Both deltamethrin and malathion concentration ...

SHEIKH AJAZ RASOOL1*, MUNAZZA DANISH AJAZ2, MUHAMMAD SALMAN RASOOL3, AHSAN SATTAR SHEIKH4 & FIRDAUS KOUSAR BUKHARI5

...associated deferment of liquid re-absorption from the gut plus invasive inflammatory manifestation along with diarrhoea. Acute or chronic or persistent campylobacteriosis are dealt with antibiotics and proton-pump inhibitors. However, an ever increasing problem of antibiotic resistance evolution has been there and that is alarming. Thus, urgency exists for finding out non-classical therapies as reduction factors against Campylobacter nuisance in humans and pou...

Salam N. Aritonang*, Elly Roza, Rizqan, Winda Habsani 

... purposive sampling techniques for determining the research location. We observed variables such as body weight, chest circumference, body length, and shoulder height. Data analysis was carried out descriptively based on arithmetic mean, standard deviation, and coefficient of variance. The result showed that the body weights of male and female buffalo crosses at <6, 7−12, 13−18, and 19−24 months were 210.26 and 196.48, 304.14 and 288.42, 3...

Muhammad Syarif Djaya1, Osfar Sjofjan2, Hartutik Hartutik2, Irfan Hadji Djunaidi2 

... using High Performance Liquid Chromatography (HPLC) for lutein content. Second, 200 day-old-Alabio laying ducks by age 4-5 months was used in this study. The basal diet was used in this study T0: basal diet, T1: basal diet + 10% lutein, T2: basal diet + 20% lutein, T3 basal diet + 30% lutein, T4 : basal diet + 40% lutein. A completely randomized design was adjusted with five treatments and six replications for each pen, consisting of ten Alabio laying ducks....

Romedi Çelik

...named because of their unique vocalizations, which sound vaguely like low laughter. The data obtained on the morphological features of angut pigeons support the hypothesis that angut pigeons are a form pigeon and should be defined as a different breed.

...
Ummul Firmani1,3, Rahmi Nurdiani2, Arning Wilujeng Ekawati2 and Happy Nursyam2*
...dentified using PCR technique and 16S rRNA gene sequencing. The probiotic potency was determined using amylolytic assay, synergistic activity, hemolytic activity, and antagonistic activity. The results showed that there were several types of bacteria found in the digestive tract of milkfish. One of these bacteria could produce an amylase enzyme with an amylolytic index of 5.16, identified as Bacillus paramycoides. This bacteria also synergized with other bacte...

Shafi Muhammad1, Bibi Nazia Murtaza2, Aftab Ahmad1, Muhammad Shafiq3, Nurul Kabir4 and Hamid Ali1*

...istochemistry (IHC) techniques in liver tissues in HCV patients from the southern districts of Khyber Pakhtunkhwa, Pakistan. In addition, the network of IL-17A and its KEGG pathway related gene IDs were constructed using GeneMANIA and STRING online web tools. IL-17A serum levels were significantly higher in fibrotic patients compared to chronic HCV patients and showed a positive correlation with age. The constructed gene network revealed interactions between I...

Lu Liu1, Xiang Zhao2, Yue Qin2, Tianxiang Gao3 and Tianyan Yang3*

...neration sequencing techniques. The complete mitochondrial genome of Lophius litulon contained 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, and 22 transfer RNA (tRNA) genes. The total length of the mitochondrial genome of L. litulon was 16,430 bp, and the overall base composition of the mitochondrial genome was 26.96% A, 30.13% C, 17.58% G and 25.33% T. Our sequence is consistent with the mitogenome (NCBI accession number AP004413) na...

Al Salihi karima Akool1* , Almas. M. Al-Bayati2, Iman Mousa Khaleel3  

...sed by histological technique and stained with Haematoxylin and Eosin. This study showed that SC’s udder appeared with four quarters, covered by brown to solid black tint skin. Both conical and cylindrical shapes of teat were observed, which constituted 75%& 25% and 37.5% & 62.5% for SCPMG & SCN-PMG), respectively. Microscopical examination of teats revealed two streak canals lined by a stratified squamous keratinized epithelium. Furstenberg&...

Salma Sharif1, Rana Arsalan Javaid2*, Abid Majeed2, Muhammad Shahzad Ahmed2, Qurat ul Ain Sani2, Faiza Siddique2, Muhammad Arshad3 and Niaz Ali

...l as novel breeding techniques for rice development.

...

Aamir Shakeel1*, Anwar Ali2, Tahir Iqbal1 and Ziad Raza1

...age Analysis (OBIA) techniques the changes in LULC can be examine. OBIA uses image segmentations on the basis of scale, colour, form compactness and smoothness. The research is an effort to apply OBIA and classification techniques to Landsat satellite images to detect the changes in LULC in Kaghan valley with range of observation of 4 years i.e., 2004 to 2008 and 2012.

...

Anwar Ali1*, Muhammad Ayaz Khan2 and Nowsherwan Zarif1

...tic random sampling technique. The total carbon stock in the study area was estimated as 1,374,702 tonnes. Out of this, 56% is in the soil, 33 % in aboveground biomass, 9% in belowground biomass and 2% in litter. Average carbon density was estimated as 100 t/ha in the landscape. Mean aboveground carbon density was determined at 33.60 t/ha. In Reserved Forest the aboveground carbon stock is 42.29 t/ha whereas in Guzara Forests, this carbon is 27.20 t/ha. Simila...

Retno Setyaningsih1, Sri Murtini2*, I Wayan Teguh Wibawan2, Surachmi Setiyaningsih2, Ekowati Handharyani3

...ELISA Kit using DAS Technique. The informations (age, sex, breed) of antibody titers against RHDV from previous study is used for samples profiling. The results showed all samples negative for RHDV antigen with average value ±0.04 (positive cut off >0.334). Seroconversion of RHDV from all seven villages where most seroconversion are showed by samples from young rabbits compare to adult rabbits, female rabbits compare to male rabbits but insignificant...

Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2

...ozoa in chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4th day of observation showed that the addition of ascorbic acid as NEZ at a ...

Irfan Safdar Durrani*, Noreen Asim and Ammar Sohail

...roteins (GLPs) are the ubiquitous family of plant proteins that belong to the cupin superfamily and have been reported to play a major role in plant defense against pathogens attacks and different abiotic stresses. Current research deals with the study of cis-regulatory elements located in the promoter region of OsGLP12-3 gene of Nipponbare and newly sequenced Oryza sativa L. cultivar Dilrosh-97 using bioinformatics approach. The possible cis-acting regulatory...

Saman Rizwan and Sohaib Aslam* 

...er two treatments were unique from each other. The results showed that all treatments enhance the carbon mineralization in soil with addition of residues compared to control. Soil treatment with incorporated residues (using 1g of residues) resulted in fastest respiration rates followed by mulched treatment. The soil treatment with incorporated residues (using 0.5 g of residues) exhibited comparatively slower rates of respiration which was understandable owing ...

Ahmad Khan1, Muhammad Amjad Ali1, Muhammad Tahir1, Samreen Nazeer2*, Muhammad Zubair Akram3*, Muhammad Arslan Azmat1 and Sabina Asghar4

Muhammad Mukhtar*, Syamsul Bahri, Syahruddin 

... the effect of applying liquid organic fertilizer on the growth and biomass production of elephant grass (Pennisetum Purpureum). This study used a completely randomized design consisting of 4 treatments and 4 replications. The study treatment was liquid organic fertilizer (LOF) from various manufactured products, namely Genetic-plus and Bio-love, and as a comparison, LOF Bio-urine produced from the biogas process. The resear...

Imtiaz Khan1*, Abdullah1, Muhammad Ibrahim1, Muhammad Ishfaq Khan1, Saima Hashim1, Shomayela Afzal2 and Khalid Nawab3 

...nal weed management techniques to maintain effective weed control in the long term.

...

Muhammad Nauman Arif1, Muhammad Mansha1* and Tanveer Hussain2

...otect Pakistan’s unique genetic resource.

...

Yanmei Wang1,2, Haoyun Li1, Lijuan Han1 and Wenkui Wang1*

...rge amount of yellowish liquid accumulated in the body cavity, and has become one of the most causes of non-infectious death in broiler industry worldwide. The macrophage migration inhibition factor (MIF) and related protein and gene expressions in its downstream signal pathways need to be studied to explore the mechanism of MIF participating in PH. In this study, 4-5 weeks broilers with clinical PH were collected as experimental (PH) group, while the healthy ...

Muhammad Ayub1*, Syed Arif Husain Rizvi2, Ishtiaq Hussain3, Musa Ali Hashmi4, Iqbal Hussain5, Shahid Hussain1, Zakir Hussain1 and Rehmat Kabir6 

...of cultural control techniques including soil racking, egg burning, and egg removal from stem crevices, were evaluated for their effectiveness in controlling this invasive insect pest. Three types of forest trees including willow (Salix wilhelmsiana M. Bieb), poplar (Populus ciliata Wall.), and Russian olive (Elaeagnus hortensis M. Bieb) were examined for mealybug population density trends and host preference. The findings indicated that implementing both soil...

Sara H. Zughayyar*, Amer H. Gyad 

...reated animals produced liquid feces. Enteropooling test findings revealed that castor oil generated an accumulation of water and electrolytes in the intestinal loop. Weight and volume of the intestine were reduced after treatment with the fruit extract of Prosopis farcta L. In contrast to loperamide, all groups intestinal contents varied significantly (P≤0.05) in volume and weight. Histopathological examinations of the duodenum corroborated the antidiarrhe...

Syed Nouman shah1, Anees Ur Rahman1, Midrar Ullah2, Shahab Ahmad Nawaz1, Muhammad Said1, Abdul Kabir1,3*, Abdul Hafeez Bukero1, Maaz Ahmad1 and Muhammad Sadiq1

...arvest and ensiling techniques, silage additives and preservatives, postharvest management practices, feed management practices, and mycotoxin binders. We found that proper harvest and ensiling techniques; silage additives and preservatives that prevent fungal growth; regular monitoring of storage conditions and feed samples; well-mixed feed formulations with mycotoxin binders; diversified feed ingredients and optimized feed...

Danish Kamal2, Muhammad Abbass Khan1, Ghulam Mujtaba-Shah1, Naveed Ahmed1, Maryam Iqbal¹, Basharat Hussain Shah1 and Imtiaz Ahmed1*

...nalyzed by standard techniques. Morphological character of various tea varieties and clones were record both qualitative and quantitative data such as stem colour stem, stem length, stem girth, number of leaves plant-1 leaf length, leaf width, leaf type, leaf apex, leaf base, leaf shape, distance between nodes, pedicel length, flower colour, and flower diameter. Tea samples were analyzed for anatomical parameters such as epidermis cells, mesophyll cells and ep...

Nusirat Aderinsola Sadiku1, Oluwatoyin Adenike Fabiyi2* and Tesleem Taye  Bello

... justify;">Pyroligneous Liquors are obtained from diverse biomasses and have been found to contain important biologically active components with high phenolic, carbonyl and organic acid contents which have several pest control properties. Bamboo Pyroligneous Liquor (BPL) has proved effective in controlling several insect pests of many crops but its nematicidal potential is yet to be fully determined. We, therefore, investiga...

Shu-Zhi Qin, Wen-Pei Ling, Mei-Fang Yin, Chun-Yu Luo and Cheng-Guo Zhao*

...re determined by WB technique. The results showed that, compared with control group, LDH, AST content, ROS level, apoptosis rate, Caspase-3, p38 MAPK, ERK1/2, JNK protein expression in H/R group were significantly increased, while cell survival rate and Bcl-2 protein expression were significantly decreased (P <0.05). Moreover, compared with H/R group, H/R+PF group could reduce the levels of AST, LDH, ROS (P <0.05), reduce the apoptosis rate and the expre...
Xiao-Wei Huang1,2, Mei-Li Liu1, Jin-Ji Wang1, Yue-Xin Liu3, Zhe Lin1, Chun-Shu Rong4* and Ji-Xiang Ren4*
...zation-high performance liquid chromatography (PD-HPLC). Wistar rats were randomly divided into 5 groups (n=10 per group): control, model, piracetam (PIR), VAPH (300 mg/kg VAP) and VAPL (200 mg/kg VAP) groups. After intragastric administration, the rats’ behavioral indexes were observed through the open field test (OFT), and the levels of superoxide dismutase (SOD), malondialdehyde (MDA), brain-derived neurotrophic factor (BDNF), and nerve growth factor ...
Akram A. Salama1, Moustafa A. Zaghloul2*, Ahmed A. Zaghawa1, Mohamed A. Nayel1, Ahmed M. Elsafey1, Mohamed F. Azooz2, Hanem S. Harb1 and Walid S. Mousa1
...cattle skin. By PCR technique, 55 samples out of 100 (55/100; 55%) were positive for LSDV. A representative sample was sequenced and had a very high identity percentage of > 99.7-100% with virulent LSDV isolated from Egypt (Sharkia Governorate) or different countries abroad (Kazakhstan, Turkey, Israel, and South Africa), while the identity with vaccinal strains of LSDV was 94.1%. The alignment analysis revealed a distinct 27-base deletion in the vaccine str...

Abdul Aziz1,2, Hamad Bin Rashid1*, Muhammad Arif Khan1, Asim Khalid Mahmood1, Ayesha Hassan1, Hamid Akbar1, Sadaf Imran1, Naveed Hussain1, Muhammad Umar1, Muhammad Asif1, Sajjad Javaid1 and Mamoona Chaudhry3

...igestion is an easy technique requiring less time for SVF isolation and gives better cell yield per gram. SVF preparation process has advantages of minimum cell contamination and processing time. Hence, it is a cost effective and alternate procedure for developing countries like Pakistan.

...

Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1

...EPL20 as Myoviruses. In liquid culture, phage virulence activity was rapid and high at a high (100) multiplicity of infection with phage SEPL01 depicting the best activity among all. Compared to the solophage suspensions at high MOI, the phage cocktail comprising all three phages did not let the bacteria regrow until 24 h overcoming bacterial resistance. All the three bacteriophages demonstrated a broad host range within the genus Salmonella (Enteritidis, Typh...

Khalid Ali Khan1,2,3* and Hamed A. Ghramh1,2,4

...Middle East due to its unique taste and quality. Pakistan exports around 4,000 tons of honey with a worth of about $ 23.00 million to Arab countries every year. It is believed that the Pakistani beekeeping industry is entering an era full of opportunities.

...
Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1, Abdur Rahman Ansari3, Faiza Yasmeen1 and Tahira Batool3
... advanced molecular techniques. 
...
Annum Razzaq1*, Zia Ullah2, Arooj Naseer1 and Abdul Nasir Khalid1
...n, Punjab, Pakistan, a unique fungus Asterophora lycoperdoides found growing on a decaying Russula species. Previously, no species of Asterophora has been described so far from Pakistan. Descriptions and illustrations are made using morpho-anatomical details. Moreover, molecular techniques have been used to amplify its ITS region of nrDNA and sequenced for phylogenetic analyses. This genus is a new recor...
Nauman Jamil Khan1, Rabia Tariq2, Hina Saleem2 and Muhammad Waheed Mushtaq2*
... Various analytical techniques including atomic absorption spectroscopy, chromatography methods, ion selective electrode method, laser-induced breakdown spectroscopy, complexometric titration method and thermometric endpoint titrimetry are developed to determine the quantity of sodium. Spectrophotometric methods are cheap, easy to handle and more precise to quantify sodium in food contents.
...

El-Kenawy, A. A. and El-Tholoth, M. S.

...munoperoxidase (IP) techniques and polymerase chain reaction (PCR) were used in our study. Chorioallantoic membranes (CAMs) of embryonated chicken eggs (ECEs) were inoculated with Known previously isolated and identified LSDV of 104 8 EID5d0.1 ml for virus follow up in these CAMs by using IF and IP techniques and PCR. The results indicate that IF and IP techniques are useful in the quick d...

Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.

... blot hybridization technique for diagnosis of bovine ephemeral fever virus.

...
Nassarl , Entsar A.; El-Dougdoug , Kh. A.; Osmanl , M.E; Dawoud3, Rehab
A. and Kinawy l , Aliaa H.*
...single local lesion technique on Nicotiana gultinosa. The induced antiserum for the isolated virus had a titer 1\1024. 600 bp DNA fragments from the coat protein gene (CP) of TMV—Ch—EG was amplified with Rt-PCR technique. Phylogenetic analysis of the TMV-Ch-EG/CP- gene showed 89% nucleotide sequence homology with other published strains of TMV in GenBank and 81% amino acid sequence homology. Tissue culture approa...

Eisa*, Nawal A.; Abd El-Ghafar **, N. Y. Abd EL-Mageed*, M.H.; Mohamed* , F.G. and Hasan*, Eman O.

...l, using enrichment technique. The phages produced plaques (4-5mm, diameter) with a distinct translucent spreading halo. Presumptive phage particles associated with X vesicatoria were observed by Transmission Electron Microscope (TEM). Particle size and morphology of each phage isolate were examined by electron microscopy. The obtained results indicated that, the isolated phages were of the head and tail types. Four phages were detected and designated A,B,C an...

Iftikhar Alam1*, Sumbal Ali1, Anwar Ali Shad2, Aneela Tahmeed1, Hafsa Nawaz1, Shumail Bakht1 and Muhammad Farooq3*

...ment is an emerging technique that seeks behavioral changes in the patients to achieve health targets. There are many challenges one may face while developing a story for health communication. A big challenge usually faced by a health story writer or developer while compiling the story maybe being evidence-based vs. staying true to the story. While understanding literary elements are essential for a health story and while there are numerous tools available to ...

Sharawil , S.S.A. and Abd El-Rahim2, I.H.A.•

...d. The isolates showed unique phylogenic analysis had not previously been identified internationally and defined as Egypt 2009. In addition; by using the obtained sequences generated from this gene coding protein; Egyptian PPRVs were grouped phylogenetically belongs to lineage IV. To the author knowledge, this is the first report describing the fusion protein (F) gene sequence, phylogenic analysis and -lineage typing of Egyptian isolates of PPRVs.

...

SHARAW11'2*S S. S. A.; AL-HOFUFY2, A. N. and AL-BLOW12, M. H.

...l-time amplificationtechniques are currently used to determine the viral nucleic acid (NA) in clinical samples for diagnostic purposes. In disease management contexts, until now, a little have been described for the molecular detection of camel pox virus (CPV). This study reports the development of a Real-time polymerase chain reaction (RT-PCR) for detection of CPV using SYBR green I chemistry. A total of 15 specimens from camels suspected of being infected wi...

Hassanein, S.A.*; Ibrahim, A.K**; Mervat, M.M.* and   Nahed,K.A.*

...e virus. Thus, both techniques can be applied for the molecular epidemiology of BHV -l.

...

*Hanan M.M. Elzahed, Abd EL-Moneim A.A, *Afaf A. Khedr, *Elham A. Elabiare, Waleed S. Abd El-Latif, Reda M.I.

...ion inhibition (HI) technique. Dams showed sufficient of antibodies to (NDV) through this period indicating that the vaccinated hens were immunopotent. No sign of immunosuppression in progeny chicks was detected by measuring (NDV) HI antibody titers pre and post lasota vaccination and by vaccination  challenge experiment, more over no (CAV) shedding was observed in progeny chicks at early stage of breeding period. In this stage CAV shedding was recorded t...

Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy

...uorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on bovine lung. Haematological examination revealed leucocytosis accompanied with neutrophilia (immature form) and lymphopenia. Histopathological examination clarified the most common lesion of field cases in lung in the form of pulmonary emphysema and alveolar collapse as well as interstitial pneumoni...

Abdel Razek,B.Omar*and Magda M.Sayed**

...luorescent antibody technique (IFAT). Serum neutralization test (SNT) and ELISA tests were applied for titration and evaluation of the hyperimmune sera before conjugation with fluorescent. Total protein concentration of the prepared LSDV antisera was 0.8g/dl. Separation of anti-LSDV immunoglobulins 1gG were done using ammonium sulphate followed by conjugation with fluorescein isothiocyanate at pH 9.6. The anti-LSDV IgG conjugated fluorescein sterile and was us...

Sabry Y. M. Mahmoud; Maher H. Hosseny and Mamdouh H. Abdel-Ghaffar

...) by tissue culture technique using different therapies, such as thermo-, chemo- and electrotherapies. Potato plants cv. Diamond cultivated at Faculty of Agriculture farm, Sohag University were tested by direct antigen coatingenzyme linked immunosorbent assay (DAC-ELISA) using antisera against PVY, Potato virus X (PVX) and Potato leaf roll virus (PLRV). The results indicated the occurrence of single and mixed infections of three viruses in potato plants. Surve...

Mayada A. Abd Elgalell; B.A Othman2; Th. Radwan, 1 and Amal S.M. Abo-Sinna3

... enrichment culture technique and signed as PPI and PP2. The phages were propagated and purified by polyethylene glycol, 6000-sodium dextran sulfate 500 two phase system. The two phages had different ultraviolet absorption spectra. Results of the lytic pattern of both phages showed that, they had a restricted host range. Particle morphology of the isolated phages was determined and it was found that the two viruses have tadpole shape, which phage PPI has an is...

E.F. Mohamed1 and I.M. Sabra2

...gically using ELISA technique. The effectiveness of gibberellic acid (GA) and indole acetic acid (IAA) against Tomato mosaic virus (ToMV) in vitro and in vivo has been evaluated. GA and IAA reduced in vitro and in vivo the infectivity of ToMV to a certain extent expressed as the number or local lesions induced by ToMV on Nicotiana sylvestris. This reduction increased gradually by increasing GA and IAA concentrations. ToMV inhibition percentages induced by GA a...

S.A. Sidaros1; R.A. Omar; S.A. El-Kewey l and Samaa Abd El-Khalik2

..., and thermotherapy techniques. Three different meristem sizes (5, 3 and 1 mm long) were excised from three garlic cultivars (Chinese. Italian and Balady). Meristems were cultured on MS medium amended with 0.5 mg/L BA (Benzyle adenine). The best size for virus elimination and survival plants was 3 mm long whereas 5 mm long produced highest survival plants without virus elimination and I mm long failed to produce survival plants in two of the tested three culti...

Seham A. El-Zeedy1, Dalia A. Abd El-Moaty1, H. A. Hussein2 and M. A. Shalaby2

...t isolate shared three unique a.a residues at positions 222A, 256I, and 294Ile that were found only in vvIBDV as well as the conserved serine rich heptapeptide region. Giza/2000 showed two additional unique a.a residues at positions 220Phe and 321Thr that resulted from two unique nucleotide substitution at positions 609 (A to T) and 911 (G to A). Surprisingly Giza/2000 shared the un

Huda M. Hamid1, Fadia W. Al-Azawi2* and Zaid F. Makki3

...sing remote sensing techniques and GIS. Due to its arid to semi-arid climate, limited water resources, and growing population, Iraq is facing significant challenges in managing water resources. Dams play a crucial role in managing the country’s water resources through storing and releasing water during periods of high and low precipitation. Understanding the hydrology characteristics of Iraq dams is essential to sustainable water resource management. In ...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

... blot-hybridization techniques were employed. Obtained results showed that 1mM isopropyl ß-D-1-thiogalactopyranoside induction at 2℃ for 18 hours was the most optimal condition for expression of recombinant GM6 T. evansi antigen. From a 500 ml of growth medium, a total of 3.4 mg of recombinant protein was successfully obtained. A recombinant GM6 antigen of T. evansi encoding a 30 kDa polypeptide was developed, which has diagnostically significant sensi...

Estabraq Hayder Khayoon, Ammar Ahmed Abdulwahid*  

... the heart puncture technique after the subjects had been given an intramuscular injection of ketamine and xylazine to induce anesthesia. The findings revealed the exposure of Acrylamide to rats significantly reduced their tendency to gain weight, a marked rise in the concentration of malondialdehyde and decrease levels of Total antioxidant in blood serum and behavioral differences like Acrylamide greatly decreased the struggling and ascending time in the ...
Saliha Bashir*, Muhammad Shafique, Muhammad Shahzad, Muhammad Sohail Anjum and Ahmad Ali Shahid
...K sample set. Based on unique iris patterns, these phenotypic traits would be helpful for individuals’ discrimination in the population. In future, there is need to explore genetic associations and functional differences of these traits.

...
Raed M. Al-Atiyat1,2*, Gamaleldin M. Suliman3, Khaled Abu-Alruz4, Firas Al-Zyoud5, Amer Mamkagh6, Ahmed M. El-Waziry7, Abdullah N. Al-Owaimer8 and Rifat Ullah Khan9
...;">The multivariate technique analysis was utilized for assigning and discriminating three Saudi sheep breeds, Naemi, Najdi and Hari based on live body and carcass traits. The traits were body height at wither and rump, body length, heart girth, body depth, head length, ear length, body weight at slaughtering, empty body, dressing percentage, hot carcass, cold carcass, and head weight which had a significant effect on breed. The phenotypic associations between...

Sarah G. Yousef1*, Ahmed Shehta2, Hend M. El Damaty1 and Hussein A. Elsheikh3

...ene-based molecular technique for MAP detection in small dairy cattle farms.

...
Asad Ullah1, Hayat Ullah2, Muhammad Lateef3, Imrana Niaz Sultan4, Afrasiab Khan Tareen4 and Muhammad Waseem Khan4,5*
...son to conventional techniques used for the diagnosis of TB. Our study is one of the first ones from Pakistan investigating and assessing the performance of GeneXpert. We recruited eight hundred clinically TB suspects initially and included seven hundred and sixteen clinically TB suspects in the final analysis. The results of GeneXpert were compared with Mycobacteria Growth Indicator Tube (MGIT) and Ziehl-Neelsen (ZN) staining. In comparison to MGIT and ZN sta...
Syed Roohullah Jan1, Kareem Akhtar2*, Uroosa1, Muhammad Zeeshan Zahir3, Abdul Shakoor4
 
...pressure cold spray technique. The powder when mixed with Mercury is called amalgam and is commonly used in dental applications for cavity filling. However, Mercury is health hazardous. In the present study coating of Ag-Sn-Cu is achieved through cold spray without adding mercury. The particle size and morphology of Ag-Sn-Cu powder are studied, before and after coatings. Hardness tests are carried out to determine surface hardness before and after the coatings...

Aalaa S. Saad1*, Taghreed H. A. Ali2, Nayerah Alatfeehy3, Dalia Elmasry4 

...ct-based biocontrol techniques have the potential to be developed to control the development of L. monocytogenes in chicken and chicken products. This result indicates that using natural and bio-based products can effectively manage diseases in the food industry.  

...

Nurul Fajeriana1*, Akhmad Ali1 and Retno Puspa Rini2

...ithout conservation techniques (plots with soil loosening only). Through the implementation of appropriate soil management and planting adapted to soil characteristics and land suitability for crop canopy development, the utilization of sloping land for agriculture must be able to support sustainable farming.

...

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

...cken spermatozoa during liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in ...

Amina1, Muhammad Zahid Rashid1*, Muhammad Asim Rashid2 and Amina Rashid2

...t-align: justify;">The unique fruit characters of new strawberry genotypes reveal their importance for the current and next breeding initiatives. The aim of present research was to assess the plant, flower and fruit characteristics of three exotic strawberry genotypes. In this respect, an experiment was conducted in the research area of the Horticultural Research Institute of Ayub Agricultural Research Institute, Faisalabad, Pakistan during the year of 2019-20...

Muhammad Tahir Latif1*, Muzzammil Hussain1, Ali Zohaib2 and Ishtiaq Hassan3

...valuate four sowing techniques, i.e., CT, happy seeder, ridge sowing and SS with Randomized Complete Block Design. As compared to CT the SS has been estimated as resource conservation technique regarding sowing time (62.50%), irrigation time (7.69%), seed cost (14%), fertilizer cost (10.81%), and fuel cost (58.33%) with the constrained requirement of higher hp tractor (preferably 85 hp) for sowing operation. An increased whe...

Muhammad Usman1*, Muhammad Uzair Khalid2, Muhammad Hasnain3*, Muhammad Tauseef4, Ali Raza4, Muhammad Akram4, Muhammad Shahid4, Abrar Ahmad4, Muhammad Shoaib Ismail1, Rabia Afzal2, Atta-Ulla2 and Muhammad Hussnain Babar3

...ng the seed priming techniques. An experiment with randomized complete block design (RCBD) with two factors on area of four-acre land was conducted. Further, four treatments have been adopted like hydro priming, osmo-priming, on-farm priming, and control under different tillage operations just like zero tillage, conventional tillage, deep tillage, and bed sowing. Result showed that osmo-priming is the best method to increase the plant height (94.74cm), tillers...

Noor A. Namaa*, Hayder A.N. Al-Zamely

...n Ginseng and creating unique NPs based on one of these components can result in the development of novel medicines with distinctive properties. Additionally, there is a clear need for greater research on the enhancement of Ginseng NPs and correct regulation of genes associated to steroid genesis and mitochondrial biogenesis.
 
Keywords | Succinate dehydrogenase, Ginseng xtract, Bicinchonic Acid Assay , Sperms, Nanoparticales, Rats...

Mahmoud Hamouda1*, Ahmed Al-Jazzar1, Fahad Al-Hizab1, Ahmed Azab2, Mohamed Al-Hammadi3

...rameters and biopsy techniques for early diagnosis in order to proceed with the appropriate treatment.
 
Keywords | Buffaloes, Uterus, Infertility
...

Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2

...se segment, there were unique bands that can be further investigated. By using species-specific primers, most fibrolytic bacteria were detected in the foregut and hindgut, whereas most non-fibrolytic bacteria were located in the foregut and midgut. This study provides a preliminary glimpse of the bacterial microbiota profile of dairy buffaloes’ gut in a qualitative and semiquantitative way. Thus, the results of this st...

Alif Rahman Rohim Puarada*, Safika, Agustin Indrawati

...ntricately shaped by a unique ecological milieu, presents a compelling avenue for exploring AMR genes’ reservoir and dissemination mechanisms. Fecal samples were collected from Papua, Indonesia. The DNA was extracted using the DNeasy Power Soil Pro Kit. A shotgun metagenomic sequencing process from the MGI DNBSEQ-G400 platform was carried out to obtain DNA sequence data from the genes in the sample. The resulting DNA sequence data were then analyzed usin...

Babi Kyi Soe1*, Toe Win Naing2, Su Lai Yee Mon1, Nay Chi Nway3, Hiroshi Sato4

...scopy and molecular techniques. A total of 59 samples were collected from Mingaladon township, Yangon region, and the presence of infection was assessed using a Giemsa-stained thin blood smear and PCR of 16S rRNA gene amplification. Both microscopic and PCR-positive samples were further analyzed for hematobiochemical alteration. As a results, Anaplasma spp. was detected in 3.38% (2/59) of sampled animals. Hematology results revealed severe anemia with a low he...

M. Imran Kasana, Rashid Iqbal Khan*, Noor Ullah Khan, M. Noman, Shahid Ali, Saima Mumtaz, Shamaila Rasheed and M. Qamar-Uz-Zaman

... different grafting techniques (Cleft, Tongue, Patch and T-budding) in ten different cultivars of avocado. The outcomes suggested that Cleft grafting was most suitable one among all other evaluated techniques. The highest survival rate (27.40%) was recorded in cleft grafting. Moreover, vegetative characters, i.e., no. of leaves and leaf area, no. of internodes and internodal length, shoot length and diameter were also higher...

Shirina Akter Toma1, Shah Ahmed Belal2, Md Abdul Baset3, Md Nazim Uddin3*

... hens has a variety of unique qualities. The current information can be useful for consumers’ healthy lifestyles for the selection of desired muscle meat from commercial spent hen and broiler.
 
Keywords | Broilers, Spent hen, Meat, Muscles, Nutritional properties, Quality traits
...

Genlong Zhan1*, Yunhua Zheng2, Desheng Li*4 and Hongmei Zhang3

...s verify the effects of Yiqi Huoxue Tongmai Decoction on patients with diabetic peripheral neuropathy (DPN). A total of 130 patients with DPN who were treated in our hospital from January 2015 to July 2016 were selected and randomly divided into a control group and a treatment group, with 65 cases in each group. The control group was treated with conventional western medicine, and the treatment group was treated with self-made Yiq

Fazal Ur Rehman1*, Saeeda Fatehullah1, Ayaz Qadir1, Suleman Ahmad2, Siddique Usman2, Muhammad Asif2, Mukhtiar Ahmed1, Sunaina Rafi3, Muhammad Mohsin2, Ayesha Amanullah4, Faisal Shahzad2, Hasnain Raza2 

...To compare surgical techniques, various hemato-biochemical, physiological parameters and post-operative complications were evaluated. The data was analyzed by applying t-test using SPSS-20 version. There was a no significant difference between both groups with respect to vital signs and hematology profile. However, seroma, abscess, stricture of anal sacs, urine incontinence, wound dehiscence and fistula formation was observed more in group A of open anal saccu...

Ediset1*, Jaswandi2, Fuad Madarisa1, Amrizal Anas1, Rizqan2

...oportional Sampling technique is 50 breeders from 5 sub-districts in Padang Pariaman Regency. Data analysis was carried out in a quantitative descriptive manner, namely the adoption rate of A.I. was measured by the percentage of adoption, and the success rate of A.I. was measured by calculating Service PerConception (S/C), Conception Rate (C.R.), and Calving Rate (CvR). The result shows the adoption rate of A.I. innovations in terms of speed, area, and quality...

MADIHA AFTAB1, ARIFA TAHIR*1, TAYYABA ASIM1 & IRFANA MARYAM2

...g these statistical techniques more than 13 folds increase in enzyme yield (4022±0.25 U/mL/min) was observed. It is concluded that cultural conditions have great influence on enzyme production and enzyme yield could be enhanced by using advanced statistical techniques.

...

ATEEQUE RAHMAN KHOOHARO* & PIRZADA JAMALUDDIN A. SIDDIQUI

...f Pakistan. Quadrat techniques were used to establish abundance of species. Overall, 49 species of invertebrates were recorded. Two of these species were annelids, 35 molluscs (Polyplacophora 1, gastropods 30, bivalves 4), 9 arthropods (cirripediae 2, malacostraca 7) & 3 echinoderms (stelleroids 3). The Highest diversity was found during the mid & high tidal zones. Diversity & distribution configurations were found to be linked with topography &...

MUSHTAQ HUSSAIN LASHARI1*, RABIA HAMEED1, UMER FAROOQ2, MUSADIQ IDRIS2 & ZIA UR REHMAN2

...his cyto-diagnostic technique is quite certain and precise as compared to that in dogs.

...

SEHRISH MUSHTAQ1, MUHAMMAD SHAFIQ1, MUHAMMAD ASHFAQ*1 FAIZA KHAN1, SOHAIB AFZAAL1, UMMAD HUSSAIN1, MUBASSHIR HUSSAIN1, RASHID MUKHTAR BALAL2 & MUHAMMAD SALEEM HAIDER1

...ontrols. Aureobacterium liquifaciens (27.66%), Acinetobacter sp. (25.66%), Bordetella pertussis (26.63%) also showed equal potential for inhibition. In contrast remaining isolates Enterobacter cloacae (19.33%), Azomonas agilis (17%) and Kurthia sp. (19%) were less efficient as compared to the others. Bacterial endophytes are colonized inside plants and have antagonistic potential for fungal pathogens. These endophytes should be further explored for disease con...

*SHAJAHAN BAIG1, MUSHTAQ AHMAD SALEEM1, MUHAMMAD AZMAT ULLAH KHAN1 ZEESHAN NADEEM2, SUFIAN AHMED2, MEMUNA GHAFOOR SHAHID3 & TANZEEM AKBAR CHEEMA3

*ARIFA ZEREEN1, ALMAS JAHAN1,SHEIKH SAEED AHMAD2 & ZAHEER-UD-DIN KHAN3

MUSHTAQ HUSSAIN LASHARI1*, UMER FAROOQ2, AMMARA ARSHAD1, MUHAMMAD NAEEM TAHIR2, MUSADIQ IDRIS2, ANAM KHURSHID3 & ZIA UR REHMAN2

...his cyto-diagnostic technique is quiet certain and precise.

...

HANNANA MARYAM, SANA MAQSOOD & SIKANDER ALI*

...ed Brevibacillus sp. in liquid culture. Soil samples were collected from different habitats of Lahore(Pakistan) for the isolation of bacterial cultures. After primary and secondary screening, ISL-4 (0.53 IBU/ml) and ISL-7 (0.6 IBU/ml) were selected having better potential for bacteriocin. A significant (p≤0.05) increase in laterosporulin biosynthesis was observed when the parameters like medium volume, buffer pH (7.0) and incubation temperature (37°C) w...

UMER YOUNAS, SHAHID IQBAL* & JAMSHED AKBAR

...assisted extraction technique. Antioxidant potentials of extracts were determined in terms of total phenolics, total flavonoids and ascorbic acid contents. In addition, radicals (DPPH• & ABTS•+) scavenging potentials were also determined. Results revealed that drying methods have no significant influence on recovery of antioxidants from sample matrix. However, methanolic extracts exhibited highest antioxidant potential that confirmed superiority ...

UZMA PARVEEN1, MASROOR ELLAHI BABAR1*, NIDA BABAR2, TAHIRA MUGHAL3 & AKHTAR ALI1

...ths. DNA extraction technique was applied. Primers were designed, amplified by PCR and RFLP were performed to identify the TNF–alpha -308 and -238 polymorphisms. The frequencies of TNF-alpha -308 genotype TNF GG, GA and AA were 37%, 10% and 3% with measurably estimation of 0.07039 (P<0.05) as indicated by HWE for the preterm and postterm conveyances while for the typical one proportion of GG, GA and AA were of 5%,10% and 5% with factually critical est...

Aziz Ahmed1*, Anila Naz Soomro2, Muhammad Ramzan Ali1, Muhammad Aleem Khan1 and Hasina Basharat1

...divided into different aliquots before applying them to in vitro fertilization. It was clear from the results of the current study that, the commercially available activation media C showed a highest significant effect on percent sperm motility i.e., 89.66%±2.88, motility interval 300sec±2.67%, Viability: 74.48±0.39% and fertilization rates: 90.04±1.70% followed by activation media A. motility: 78.24±1.90, interval: 280sec&pl...

Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1

...en was then stored in a liquid nitrogen container at a temperature of -196°C.After 24 hours, the semen was thawing at a different temperature. The evaluation of semen quality included motility, viability, plasma membrane integrity, and acrosome integrity. All data obtained were analyzed with two way-analysis of variance. The result showed that equilibration time treatment and differential temperature of thawing yield good results on the motility and viabil...
Muhammad Atif Raza1, Muhammad Tariq Javed2, Muhammad Fiaz1*, Muhammad Shakeel3, Muhammad Shahbaz Ul Haq2, Amna Kanwal2, Syeda Maryam Hussain1 and Muhammad Zubair Siddiqi4
...esign through ANOVA technique. Mortality percent was found minimum 13.3% in birds under T3 group. Effect of all nanoparticle levels was not different (P>0.05) to that of antibiotic in terms of total serum protein and lipid profile; total glycerides, very low-density lipids, high density lipids and total cholesterol. It was concluded on the basis of findings of current study that nanoparticles zinc oxide and copper oxide mixture (37.5 + 15 mg/Kg/d) was found...

Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain

...abies, conventional techniques such as Direct Fluorescent Antibody Test (dFAT) and histopathology are still employed. The OIE and WHO both frequently propose the gold standard test, the Direct Fluorescent Antibody Test (dFAT), for the diagnosis of Rabies in fresh canine brain tissues. The preferred methods for routine diagnosis include PCR (Polymerase Chain Reaction), and Mouse Inoculation Test (MIT). In endemic locations, vaccinations with DNA, recombinant va...

Tanveer Jilani1*, Irfan Khan2, Asmat Salim2, Fareena Bilwani1, Bushra Moiz3 and Mohammad Perwaiz Iqbal1,4

...s compared to other techniques. Human EPCs differentiated by this protocol can then be used to study normal and abnormal erythropoiesis.

...
Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
... humans. The thirds technique involving administering hormones has been found to be most effective method but this too have some significant side effects. Alternatively, natural products can potentially serve as an affordable and effective substitute for the treatment of MS with minimum or no side effects. Blackseeds (Nigella sativa) and honey possessing potent neuroprotective, antioxidant, and anti-inflammatory properties with no reported side effects can be ...

Zuhair Dardona1,2, Adnan Al-Hindi3, Mohamed Hafidi1, Ali Boumezzough1 and Samia Boussaa1,4*

... both ELISA and PCR techniques. For this purpose, 60 red meat samples were collected from butcheries in Gaza city, during the period from January to March 2021. These samples were divided evenly between beef and mutton. This study found that beef is devoid of T. gondii, whether tested using ELISA or PCR. On the contrary, both approaches detected T. gondii in mutton; however, the percentage of positive samples reported differed. For example, whereas T. gondii w...

Muhammad Asif Iftikhar1, Talat Naseer Pasha2, Saima Inayat1, Khalid Javed3 and Rahman Ullah4*

...gated through ANOVA technique under complete randomized design (CRD) using SAS 9.1 software. The results showed that yoghurt made from Nili Ravi buffalo milk (To) showed a significant (p≥ 0.5) overall acceptability as compared to other treatments. Yoghurt made from Nili Ravi buffalo milk (To) were followed by T3, T4, T1 and T5 respectively. The Nili Ravi buffalo milk showed higher acceptability to the consumers.

...
Luis Fernando Díaz-Toral1, Carlos Eddy Alvarado-Zamora1, Ernesto Gonzalo Cañarte-Bermúdez2*, José Bernardo Navarrete-Cedeño2, Gilmar Jesús Cañarte-Cañarte1 and Fernando David Sánchez-Mora1* 
...ose of application of mepiquat chloride (MC), as a plant growth regulator (PGR), in the Coker cotton commercial variety. This field trial was carried out during February-August 2020 in La Teodomira experimental farm of the National Institute of Agricultural Research (INIAP, Lodana, Manabí, Ecuador), with three periods of application and four doses of growth regulator in addition to control (without regulator). A randomized complete block design in addit...
Yanis Cruz-Quintana1*, Ana María Santana-Piñeros1, Byron Manuel Reyes-Mero1, Leonela Griselda Muñoz-Chumo1 and Lenin Cáceres-Farías1,2 
... paraffin inclusion technique, to evaluate the damages associated with parasites. Trichodina heterodentata was detected in specimens of P. brachypomus from the farm located in El Coca, with prevalence of 100%, and mean intensity of 0.21 ± 4.7 parasites per host. Histopathological analysis showed the presence of slight hyperplasia and thickening of the lamellae of parasitized fish. The fish from El Coca farm showed significantly lower avera...

Evy Rossi1*, Fajar Restuhadi1, Raswen Efendi1, Yusmarini1, Rahmayuni1, Emma Riftyan1, Bisma Panca Winata2, Yolanda Ashara2, Usman Pato1

..., which can be found in liquid or powder form in microencapsulated form. This powdered yogurt starter will be applied using back-sloping fermentation for the practicality of the yoghurt-making process. This research aimed to determine how many cycles the back-sloping fermentation using microencapsulated yogurt (MY) starter was applied and evaluate the quality of yogurt in each back-sloping fermentation cycle. The lactic acid bacteria contained in MY starter we...

Muhammad Ali Raza1*, Aneela Zameer Durrani2, Bilques Bano3, Muhammad Muddassir Ali4, Nazia Rubab5, Syed Tasadak Mehdi6, Muhammad Wasim Iqbal7, Kumay Hassan Akhtar8, Aqeel Raza9, Ujala Fatima Shan10, Muhammad Aftab11

...ry and applied HPLC technique on samples and found residues of Penicillin and oxytetracycline in different percentages (11% and 9%, respectively) and percentage of both combine Penicillin and oxytetracycline is 3%. In a yoghurt sample, the highest penicillin was found as 13.95 µg/Kg, the lowest penicillin was detected as 7.07 µg/Kg, the highest oxytetracycline was observed as 306.73 µg/Kg, the lowest oxytetracycline was figured out as 197.77 ...

Jinzhao Li1, Yawen Zhang2, Binghan Jia1, Yuqiong Zhao1, Huijuan Luo1, Xiaojie Ren1, Yuan Li3, Xiaoyan Bai1, Jing Ye3 and Junping Li1*

...To conclude there is a unique distribution pattern of tau within the distal colonic musculature of OIC rats. The abnormal activation of MORs contributes to the hyperphosphorylation of tau protein, which in turn induces the development of OIC.

...

Pakistan Journal of Zoology

April

Pakistan J. Zool., Vol. 56, Iss. 2, pp. 503-1000

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