Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6
...nfected animals (rat and horse, respectively). BDV proteins and their interactions with host proteins could be shown in cell cultures (HMGB1, Cdc2) and in brain tissues of rat (HMGB1, Cdc2) and horse (Cdc2 only) infected with BDV. In this study, we have for the rst time directly visualized protein-protein interactions between BDV and its host, and thereby con rmed previous data to demonstrate ndings in cell cultures to be ...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...>Leptospira positive horses based on ELISA and 9 healthy horses were included in the study. Blood profile, serum biochemistry and mineral profile were analyzed at day 0, 7 and 21 of infection. Results showed significant decrease (P<0.05) in red blood cells (RBC), packed cell volume (PCV), hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC) and platelets while total leucocytes count (TLC), neutrophils, eo...
Mohammad Salim1, Arshad Javid2,*, Faiz-ur-Rahman3, Khalid Javed Iqbal4 and FarmanUllah5
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
... broth supplemented with horse serum. The blood was used for serum plate agglutination assay. Thereafter, the samples were subjected to genomic DNA extraction to detect Mycoplasma spp. using multiplex-PCR. Isolation and identification data showed that a total of 58/96 samples (60.41%) were positive for Mycoplasma infections whereas a total of 61/96 blood samples were also found positive using serum plate agglutination. However, the multiplex-PCR detected Mycop...
Yasemin Bircan Yildirim* and Hediye Tugce Vurmay
...ue (Boops boops), horse mackerel (Trachurus mediterraneus), red mullet (Mullus barbatus), brushtooth lizardfish (Saurida undosquamis), grey mullet (Mugil cephalus), threadfin bream, (Nemipterus randalli), which were caught from the Iskenderun Bay at 6 different locations (local fishermen’s fish market) were evaluated. The Serratia spp., Enterobacter aerogenes, Klebsiella pneumoniae, and Enterobac...
Ikramul Haq1, Aneela Zameer Durrani*1, Muhammad Sarwar Khan1, Muhammad Hassan Mushtaq2, Imtiaz Ahmad3, Amjad Khan2 and Mehboob Ali1
...tudy 294 faecal samples (horse n=217, mules n=23 and donkey n=54) were diagnosed positive for bacteria. The overall prevalence of diarrhea was 72.56% with bacteria in 65.77% of diarrheic cases. The prevalence of different bacterial species shows that E. coli was the most prevalent bacteria with 48.77% as compared to Clostridium perfringens (18.56%) and Salmonella (17.9%). It was concluded that bacteria were the major cause of foal diarrhea...
Muhammad Jamal Khan1, Abdul Malik2*, Muhammad Shahzad Khattak2, Naveedullah1, Naeem Ijaz3 and Ishaq Ahmad4
...from 2 to 14 l/s and the horse power of the pumping units ranged from 2 to 30 hp and the pumps efficiencies of the wells ranged from 22 to 73% with an overall average of 42%. The number of hours the farmers operates their well ranged from 2 to 10 hours per day. The water quality reveals that pH of the water varies from 8.2-8.9 while ECw ranges from 0.4-4.7 dS/m. Out of 36, only inseven villages groundwater has ECw higher than the useable limit recommended by W...
Nadia Saeed1,Mian Sayed Khan2, Habib Ahmad3, Muhammad Abdullah4 and Muzafar Shah5,*
...mendments pigeon manure, horse dung, cow dung, duck manure and castor oil cakes at the rate of 8 Kg/tree and saw dust at the rate of 10kg/tree, respectively. Untreated trees were kept for the comparison. Soil samples and sub samples of both treated and untreated trees after 3, 6 and 12 month from Abbottabad and Mansehra by using Bearmann funnel technique. After Quantitative analysis of both treated and untreated trees, it was observed that treated trees sample...
Danyu Zhang1, Ying Liu1, Qinxin Shi1, Zhiwei Peng1, Yan Hua1 and Zhijun Hou1,2,*
...n Siberian tiger, panda, horse, and human, and it may originate from the more divertible diet of the sable.
...
Muhammad Ijaz1, Shahid Hussain Farooqi1, Rahmatullah2, Amjad Islam Aqib1, Sadaqat Ali3,*, Awais Ghaffar1, Ahmad Ali1 and Sehrish Saleem1
... diarrheic foals (n = 35 horse foals, n = 35 donkey foals, and n = 35 mule foals) were selected regardless of their etiologies. Additionally, 12 healthy foals (n = 4 horse foals, n = 4 donkey foals, and n = 4 mule foals) as control animals were selected for this study. Packed cell volume (PCV) and serum electrolytes, trace and ultra-trace elements were measured in both groups (diarrheic and healthy). The results showed signi...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
...netic diversity among 12 horse breeds present in Pakistan was determined by using RAPD markers. The blood samples of purebred animals of eight exotic breeds (Thoroughbred, Arab, Anglo-Arab, Suffolk, Percheron, Clevland, Noriker and Hanovarian) present in Pakistan were collected from Army Remount Stables while those of four Pakistani indigenous breeds (Morna, Saen, Kakka Bralanwala and Anmol) from local horse breeders of Punj...
Muhammad Raza Khan1, Tariq Mahmood1,*, Hira Fatima1, Faraz Akrim2, Shaista Andleeb1 and Abdul Hamid1
...consuming mainly donkey, horse, cow, sheep and goats, resulting in a negative perception among the locals. Most of the depredation occurs at night time while maximum livestock are depredated during winter season.
... 
Muhammad Shuaib Khan1, Shakeeb Ullah1, Nisha. A.R2, Muhammad Umar3, Muhammad Inamullah Malik1*, Syed Muhammad Kamal Shah1, A. Zaman1, Shakir Ullah1, Imdad Ullah1, Sumera Ali Khan4
...i.e. camel, bull, horses/ mules and donkey. Score from female respondents by different pack animals i.e. camel, bull, horses/ mules and donkey receive were 33.67±2.55, 29±1.00, 29.52±2.52 and 58.67±2.082 respectively (% means ± Standard deviation), while from male respondents with the % means ± Standard deviation of 34.67±6.43, 35.00±1.00, 36.00±2.6...
Sumaira, Ali Mujtaba Shah*, Ghulam Asghar Solangi, Ifra Anwar, Qudratullah Kalwar
...es including sheep, ass, horse, yak, goat, bison and camel while the 80 % milk is produced by cows. Milk of camel plays an essential part in the diet of human. Additionally, camel milk comprises numerous fatty acids and enzymes. Hence camel milk has many beneficial effects, such as antiviral, antibacterial, anti-diabetic, anti-carcinogenic and anti-ageing. Besides, camel milk contains abundant proteins which are conductive to improve the immunity functions. Th...
Moazam Ali1, Wajid Ali2, Ayhan Ceyhan2 and Zeeshan Ahmad Bhutta3*
Pigmentation Genome Influence in Animals and Human Interventions in its Course of Action
... species (sheep, cattle, horses, camels, dogs, cats, pigs) new variants of coat pigmentation are achieved by generating mutation in MC1R and ASIP allele. A row of scientists is working on genome sequences and mutations for getting a better and healthier pigmentation besides animal welfare. This review article contains a complete elaboration about the worth of pigmentation in animals, natural pigmentation process and new mutation and deviation in animal genome ...
Małgorzata Dzierzęcka1, Sławomir Paśko2, Marcin Komosa3, Karolina Barszcz1, Bartłomiej Jan Bartyzel1 and Ewa Czerniawska-Piątkowska4*
...y analyzed the impact of horse age and body weight on the angle between the parietal surface of the coffin bone and the ground. A precise description of the plantar margin and the inclination of the parietal surface of the bone may contribute to a better understanding of the biomechanics and strength of the hoof, in particular in horses with higher body weights. The study material included coffin bones of the thoracic limb, ...
Muhammad Tahir Waseem1, Abdul Majid Khan1*, Abdul Ghaffar2, Ayesha Iqbal1 and Rana Manzoor Ahmad1,3
...ich represent that these horses dominantly inhabited C3 vegetation however, the C4 grasses were being indicated as the diet of Hipparionines the latest Miocene. All these species are believed to inhabit more open environment with patchy grasslands. Non-significant differences were observed between the species which explains that the dietary niche of these species was more or less similar. However, the values for carbon isotopes are highly...
Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3
...ces of Serbia. Breeds of horses, donkeys, cattle, buffalo, sheep, goats, pigs, chickens, geese, turkeys, ducks, guinea fowls, dogs and bees were analyzed. The number of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic resources, be included in the Decree in the future, in order to p...
Iftikhar Hussain1, Sardar Azhar Mehmood1, Muzafar Shah2*, Mohammad Salim3, Shabir Ahmed1, Sana Ullah1,Sidra Batool4, Nadia Saeed1 and Khalid Usman1
...inx and C. horsefield was supported by 83% bootstrap value. The relationship between C. titthaecheilus and C. horsefield was supported by 98% bootstrap value. These analyses confirm the utility of the COI gene sequence of C. sphinx, obtained in the present study for species level identification and phylogenetic relationships.
...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...ility of frozen semen in horses. Seven stallion were used for semen collection in this study. A total of twenty ejaculates was collected from three healthy stallions twice a week for the experiments 1 and 2. All ejaculates were divided into two parts of ten ejaculates each, ten ejaculates for cysteine in experiment 1 and the other ten for ascorbic acid groups in experiment 2. Each ejaculate from an individual horse was divid...
Ahmed F. Afify1, Mohamed A. Shalaby2, Ahmed A. El-Sanousi2 and Amal S. Gaber1
...private studs of Arabian horses
and also foreign horse breeds, including 540 serum samples, among these, 4 EDTAblood
and 4 semen samples. Serologically, indirect ELISA was performed on 540
serum samples. 130 samples were clearly strong positive at a percentage of 24%.
Molecular detection of EAV genome was performed only on 8 selected semen and
EDTA-blood samples whi...
Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3
...our study, three Arabian horses from Cairo, Egypt, were died after nervous manifestation expecting Rabies. Brain tissues were sent for direct fluorescent antibody technique and isolation on tissue culture using BHK cells. Direct confirmation was performed by Real time RT- PCR and gene sequencing was performed directly from brain samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conduct...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
... anti equine IgG labeled horse radish peroxidase, concluding that the expressed envelope glycoprotein D of EHV-1 (EHV-1gD) could be used as a good candidate for manufacturing of diagnostic Antigen for detection of the circulating antibody either in vaccinated or latent infected horses....
Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1
...(EHV)-1 is a pathogen of horses, well known for its ability to induce abortion and nervous system disorders neonatal foal pneumonitis, highly contagious among susceptible horses, with viral Transmission to cohort animals occurring by inhalation of aerosols of virus-laden respiratory secretions. Extensive use of vaccines has not eliminated EHV infections, and the worldwide annual financial burden from these equine pathogens i...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...iv>
domestic pigs, horses, and numerous bird species. Maternal derived antibodies could interfere with the
efficacy of vaccination against avian influenza in early age in chicks.
Methods: Eight groups of one-day old commercial broiler chicks were kept in isolators along the whole
period of the study. Groups 1, 2 and 3 were vaccinated with the prepared vaccine; group 4, 5 and 6 were
vaccinated with one of th...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...ntly (silently) infected horses is the main cause of circulation of such virus in horses.
Methods: In the present study, molecular characterization of circulating EHV-1 viruses among horses
of equestrian clubs in Egypt was carried out. Sixty-two samples of whole blood with anticoagulant
were collected and screened for EHV-1 using nested PCR that amplify ...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
Wei Luo 1,2,3, Yongtao Jin 1,2,3, Xuqing Li 1,2,3 and Ke Liu 1,2,3*
...Tibetan sheep, yaks, and horses, and three kinds of wild herbivores: Tibetan antelopes, Tibetan wild assess, and blue sheep.All the kinds of large herbivores in the aerial images are detected and located using deep learning model of the MASK R-convolutional neural network (CNN), and the average recall, correct, and leakage are 89%, 98.4%, and 10.8%, respectively. Furthermore, the contour vector of the herbivores is obtained by extracting the mask generated in ...
Mary A. Sargious1, Hanaa A. Ahmed1, Ragab M. El-Shawarby2, Hatem H. Bakery2, Sherif I. Ramadan3, E. Gus Cothran4 and Ayman Samir Farid5*
...">A total of 229 Arabian horse hair samples including 120 samples from El-Zahraa stud and 89 from two private farms in addition to 20 hair samples of Dutch Warmblood were genotyped by 16 microsatellite markers. The purposes of this study were; firstly, to investigate the current status of the genetic diversity and inbreeding of Arabian horse populations reared in Egypt. Secondly, to examine the traditional maternal based str...
Grace Chinenye Onyishi, Chinedu Ifeanyi Atama*, Fejiokwu Esther Chioma, Ifeanyi O Aguzie, Godwin I Ngwu and Christopher Didigwu Nwani
...parasites of donkeys and horses of Udenu Local Government Area (LGA), Enugu state, Nigeria. Standard procedure of parasite search in vertebrates was used. A total of 23 donkeys and 24 horses were examined for gut helminth parasites. In total, out of 47 equines examined, 43 (91.5%) were infected. Out of 24 horses, 20 (83.3%) were infected while all the donkeys were infected (100.0% prevalen...
Alaa Jaheen*, Noha Salem, Mohamed El-sherif
...recurrently manifests in horses, especially in the summer season due to insect bites. This study aims to evaluate the hematological alterations, oxidant/antioxidant changes, and protein and lipid profiles in horses suffering from equine eczema. Thirty (30) horses were included in this study (20 males, 10 females), classified into the healthy control group (n = 10) and the equine eczema gro...
Uzochukwu Ephraim Emeto, Chukwuemeka Calistus Okolo* and Nwakaego Ernestina Nweze
...nd welfare challenges on horses. In countries such as Nigeria, where control of these endoparasites relies on periodic use of anthelminthics, epidemiological evaluations are necessary for deploying meaningful intervention strategies. Such investigations are lacking in the region; hence, the occurrence, risk-factors and nature of cestodiasis and nematodiasis of horses at Obollo-Afor southeastern Nigeria were investigated.
Iqtidar Hussain1, Haroon Shahzad2*, Sami Ullah2*, Muhammad Jawad Nazir1 and Muhammad Rizwan3
Mostafa El-Sebelgy1*, Hanafy Madbouly2, Sabry Tamam2, Nagwa Ata1, Kawther Zaher1
Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2
...outbreak was reported in horse studs, Egypt, in 2019. Investigating the role of EHV-4 in this abortion outbreak in mares using various molecular detection techniques were done using the tentative diagnosis by rt-PCR. The presence of 16 liver and spleen samples out of 20 (80%) from aborted fetuses were positive for EHV-4, but all were negative for EHV-1. Virus isolation trial for EHV-4 were done, eleven samples out of 20 (55%) on the CAM of ECE were positive. T...
Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*
...nd identified in humans, horses, and bovines, but rarely in ducks. We isolated and cultured cartilage stem/progenitor cells (CPSCs) from the articular cartilage of 14-day-old Peking duck embryos using the fibronectin adhesion method. The morphology of CPSCs was observed under an inverted microscope. Based on the growth curve test, we found that the growth mode of the cells was “S” type growth. The clonogenic assay was performed to determine the clo...
Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4
...n of 1:6400 with sera of horses and donkeys infected with trypanosomosis. The proposed test system can be used for diagnosis of trypanosomosis in the early stages of the disease. ...
Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2
...wound healing in rescued horses. Twelve horses were divided into two groups: group A (PRP-treated) and group B (control). The group A (n=6) animals were treated with autologous PRP gel, while the group B (n=6) animals were treated with sterile saline. Full-thickness (3 x 3 cm2) skin wounds were selected in each horse on either left or right region of back. Neovascularization was assessed o...
