Aneela Qureshi1, Ammara Ainee1*, Muhammad Nadeem1, Masooma Munir1,2*, Tahir Mahmood Qureshi1, Saqib Jabbar2
...ifferent proportion with grape fruit albedo powder. The effect of fiber source on physicochemical, sensory attributes and microbiological properties of fruit cakes were examined during 30 days of storage. Results showed that with varying fiber content water activity, texture, color, moisture, fat, protein, ash, NFE and fiber content changed significantly (p<0.05). Gross energy level in cake which is desirable by consumers was reduced but high fiber cake was...
Rana Muhammad Aadil1,2*, Xin-An Zeng1, Saqib Jabbar3, Akmal Nazir2, Abid Aslam Mann2, Muhammad Kashif Iqbal Khan2, Abdullah2, Amna Ramzan2
...me quality parameters of grapefruit juice were investigated based on pH, acidity, ºBrix, viscosity, cloud value, hunter color values, bioactive compounds, microorganisms and enzyme analysis. The grapefruit juice was treated with processing variables of HPP treatment (0, 150, 200 and 250 MPa), temperature (40, 50 and 60 oC), for processing time of 3 minutes and thermal treatment was done in a water bath at 70 ...
Abbas Ullah Jan1, Muhammad Fayaz1*, Yousaf Hayat2, Dawood Jan3 and Ghaffar Ali3
...e is constant for dates, grapes, guava and mango and tends to increase in all fruits, apple, banana and citrus with increase in household income for urban households. For rural households with increase in household income, quantity elasticity of all the fruits tends increase with the exception of grapes where it decreases and remains constant in dates. The estimated quality elasticities reveal that both the urban and rural h...
Justyna Batkowska1,*, Lukasz Wlazlo2, Kamil Drabik1, Bozena Nowakowicz-Debek2, Karrar I.A. Al-Shammari3 and Magdalena Gryzinska1
...) after applying red grapefruit juice (Citrus paradisi) for hatching eggs disinfection. 420 eggs were randomly divided into 3 groups (150 pcs each). 1st group was not disinfected (NC – negative control), the 2nd one was disinfected with formaldehyde and KMnO4 fumigation (PC - positive control). In 3rd group fresh juice of red grapefruit was used as disinfectant (GT...
Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*
COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
...ign: justify;"> The grape (Vitis vinifera L.) is the most valuable and widely cultivated horticultural crop worldwide. In the present study, different hormonal combinations in regeneration media were studied to determine the best combination for in vitro morphogenesis of grapevine accessions viz., 4494(1) Kowar Sufaid, 4472(12) Harker Agoon and 4492(1) Kowar Naray. When levels of benzylaminopurine (BAP) were increased i...
Muhammad Ali*, Ghulam Mustafa Sajid**, M. Faisal Anwar Malik*** and Kalimullah****
ESTABLISHMENT OF IN VITRO CULTURE OF GRAPES
...(meristemetic tissue) of grapes was investigated through tissue culture technique. These explants were collected from gene bank of Institute of Agricultural Biotechnology and Genetic Resources (IABGR), National Agricultural Research Centre (NARC), Islamabad, Pakistan. Fifteen accessions of grapes were surface sterilized and tested on 75% MS media for germination and initial growth parameters. Accession No. 020017 (Dakh-1) ex...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
..., polygalacturonase from grape skin was purified by salting out with ammonium sulfate, gel filtration on Sephadex G-75 column and ion exchange on Q-Sepharose column chromatography. Polygalacturonase was recognised as a protein with 47kDa molecular weight by SDS-PAGE. The optimum pH of purified polygalacturonase activity was found to be 4.5 and stable within pH range 3.5-5.5. Temperature dependent studies revealed temperature optimum of enzyme to be 40°C an...
Muhammad Arshad1, Muhammad Irfan Ullah1*, Muhammad Afzal1, Mian Anjum Murtaza2, Ejaz Ahraf3, Zahoor Hussain4, Syed Muhammad Ali Zahid1 and Maryam Riaz1
Chandragouda M. Patil, Shashikant S. Udikeri* and Shreeshail. S. Karabhantanal
...Koch population from grapevine orchard indicated considerable survival on dicofol 18.5 EC, fenpyroximate 5 SC, diafenthiuron 50 SC, sulphur 80 WP, abamectin 1.9 EC, hexythiazox 5.45EC, spiromecifen 240SC, propargite 57% EC, ethion 50EC, fenazaquin 10%EC treated leaves. Field populations had high degree of resistance to Sulphur 80WG with LC 50 of 17769.72 ppm against 651.17 ppm for a laboratory susceptible strain. Thus 27.30 fold resistance ratio...
Ummad ud Din Umar1, Syed Atif Hasan Naqvi1*, Ishfaq Ahmed1, Ateeq ur Rehman1, Muhammad Asif Zulfiqar2, Sibghat Ullah3, Shah Pasand4, Zehri Khan3 and Abdur Rehman5
Prevalence and Serological Detection of Spiroplasma citri, a Cause of Citrus Stubborn Disease in Southern Punjab, Pakistan
...idence. Sweet orange and grape fruit depicted themselves as susceptible ones showing maximum disease with “18.33” and minimum “02%” respectively. Kinnow mandarin and feutral’s early appeared as resistant cultivars against CSD infection in all the surveyed areas. Small acorn shaped, lopsided fruit and leaves with shortened internodes were appeared to be characteristic disease symptoms in Citrus stands of Southern Punjab....
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...d day and quickly formed grape-like clusters that were consistent with the morphological features of SSCs. These cells were identified as positive by immunofluorescence staining. This study successfully isolated, enriched and identified buffalo SSCs and established an effective platform to explore the mechanisms of proliferation and differentiation of buffalo SSCs.
...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1, Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...Citrus sinensis), grapes (Vitis), and peach (Prunus persica), respectively. Utilizing yeast extract, peptone and dextrose maximum biomass was produced by S. cerevisiae isolate recovered from peach (SCP-1) i.e. 4.83 g/l. Using isolate SCP-1 higher biomass of S. cerevisiae was produced on sugarcane molasses (6.42 g/l) followed by orange peels. Microbial biomass produced on the sugarcane molasses was rich in crude protein...
Allah Bakhsh1, Attiq Akhtar1*, Fiaz Hussain1 and Shabbir Ahmed2
...gnificant yield loses to grapes yield every year. Fruit Zone Leaf Removal (FZLR) is a canopy management technique that provides a cost-effective and high-quality grape yield. In order to boost grape quality and minimize disease incidence in the King’s Ruby variety, FZLR was used for two seasons in a row. Total leaf removal was used before bloom, during full bloom, and four weeks afte...
Om-Hashem M. El-Banna1, Maisa A.E. Awad2, M.S. Abbas3, Hoda M. A. Waziri2 and Huda S. A. Darwish2
...erious viruses affecting grapevine, tomato and other hosts. The virus was isolated from infected grapevine which was characterized by ring spots, mottling and shortening of internodes. And from tomato exhibiting necrotic rings and sinuous lines symptoms. The virus was checked in the suspected samples by ELISA using the specific antiserum. Anatomical and ultrastructure changes in leaf tissues of both Tomato and
Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...to,
tobacco, grape, apple, peach, cherry, apricot and raspberry. Both of sodium azide (NaN3) and ethyl
methanesulfonate (EMS) are known as chemical mutagens.
Objective: Study the effect of chemical mutagens (NaN3and EMS) on the resistance of (ToRSV) on
two different tomato cultivars and characterization induction mutagens by ISSR molecular method.
Methods: ToRSV was isolated from infected tomato,
Hamza Tariq1, Nasir Ahmed Rajput1*, Muhammad Atiq1, Shahbaz Talib Sahi1, Abdul Rehman1, Abdul Rashid1, Muhammad Ahsan Khan2, Akhtar Hameed1,3 and Warda Modassar Saddique1
Ameliorative Effect of GSPE Against AFB1 Induced Immunotoxicity and Hepatotoxicity in Japanese Quail
Maha Khalil1, Gamal Shams2, Hosny Abdel Fadil2, Nagah Edrees2, Mostafa Abonorag1, Nasser El-Sabbagh3, Eman A. Ahmed4*
...to assess the ability of grape seed proanthocyanidin extract (GSPE) to alleviate the detrimental effects triggered by aflatoxin B1 in Japanese quail. Four groups of Japanese quail birds were fed either a basal diet (control), basal + 1mg/kg AFB1 (AFB1 diet), basal+ 500 mg/kg GSPE (GSPE diet), or basal+ 1mg/kg AFB1+ 500 mg/kg GSPE (AFB1+GSPE diet) for 5 weeks. Biochemical parameters, lipid peroxidation, pro-inflammatory cytokines, and histopathological investig...
Muhammad Ismail1,*, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1 and Umair Abbas1 and Riaz Hussain2
...e (Citrus aurantium L.), grapefruit (Citrus paradisi Macfad), lime (Citrus aurantifolia Christm), mandarin (Citrus reticulata Blanco) and sweet orange (Citrus sinensis (L.) Osbeck) against B. dorsalis using choice and no-choice fruit-dip bioassays. Results revealed a significant reduction of pupal weight and adult emergence of B. dorsalis by 4% extracts of A. indica, A. sativum and C. aurantifolia. Moreover, a significantly higher male to female adult sex rati...
Monis Hussain Shah1*, Rizwan Rafique2,6, Munawar Almas1, Muhammad Usman3, Sadia Yasin4 and Sajida Bibi5
H. M. Hassan, M. M. Tantawy*, A. M. Younes and M. O. Sayed
...ring fruiting seasons of grapevine in 2012 and 2013. The nematodes encountered were identified as Meloidogyne spp., Helicotylenchus spp., Longidorus spp., Pratylenchus spp., Tylenchulus semipenetrans and Hoplolaimus spp. Community analysis of these plant parasitic nematodes showed variability in nematodes population densities, frequencies, biomass and importance values in different Districts during 2012 and 2013. Root-knot nematodes Meloidogyne spp. was the mo...
A. Khan†, S.S. Shaukat*, N. Khatoon**, J. Akhtar and A.G. Rizwana**
...edlings and rootstock of grapevine were selected from 6 vineyard from two districts of Balochistan,
Pakistan. Seven species of nematodes were found associated with the seedlings/rootstock including Aphelenchus avenae,
Helicotylenchus indicus, Hoplolaimus indicus, Meloidogyne javanica, M. incognita, Xiphinema americanum and X. index.
A matrix of similarity between six vineyard-nurseries was computed using Bray-Curtis index.
Najam-ul-Sehar Afshan1*, Javeria Majeed1, Abdul Rehman Niazi1, Saima Khanum2, Maria Riaz1, Muhammad Fiaz2 and Shaila Anjum1
...ausing powdery mildew on grapevines is being reported in Pakistan. Infected plants were collected from districts Mansehra and Abbottabad of Khyber Pakhtunkhwa, and district Lahore of Punjab, Pakistan, during phytopathogenic surveys in 2015–2020. The causal fungus was observed and identified on the basis of morpho-anatomical and molecular analyses. This is the first report of powdery mildew infection caused by Erysiphe necator var. necator from Punjab and...
E. K. Allam.1; B. A. Othman.1; Hayam S. Abdelkader2; and Noher A. Mahmoud2
...solated from symptomatic grapevine (Vitis vinifera L.) leaf samples obtained from El Kalubia Governorate. Three out or 25 samples were positive for GFLV by DAS-ELISA using specific polyclonal antibodies raised against purified GFLV preparation and five samples were positive by RT-PCR. RT-PCR detected GFLV in both fresh and dried tissues. A fragment (321 bp) of the coat protein gene of GFLV was amplified by reverse transcription-polymerase chain reaction (RT-PC...
Mohamed Adam1*, Hassan Sobhy2, Mohamed Abouzid3, Dalia Elhafny4 and El-Desouki Ibrahim5
...Meloidogyne incognita on grape, in comparison with chemical nematicides: Nemacab (ethoprophos 20% Ec), Dento (fenamiphos 40% Ec) and Vaydate (oxamyl 24% Ec) during two successive seasons (2016 and 2017) under field conditions. Drenching of soil with a suspension of all tested products significantly reduced the nematodes populations in soil compared with the untreated control in both trails. Among chemical and bio-nematicides, there was no significant differenc...
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
...a natural source such as grapefruit was effective in combining the antioxidant and anti-inflammatory properties of both the flavonoids present in grapefruit and selenium and increasing the efficiency of the selenium nanoparticles....
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
...a natural source such as grapefruit was effective in combining the antioxidant and anti-inflammatory properties of both the flavonoids present in grapefruit and selenium and increasing the efficiency of the selenium nanoparticles....
Xiang Li1, Zhijun Zhao1, Lei Zhang2, Jinhuan He1, Fengling Li1, Jing Li1 and Chunmei Pan1*
...f fermented feed (taking grape pomace residue as example) on the nutrient apparent digestibility of piglets. Through the determination of tannin content in grape pomace residue, the nutritional value of grape pomace residue was evaluated, and the influence of feed with different grape pomace residue content on the nutrient apparent digestibility of pigle...
El-Dougdougi, Kh. A.; Dawoud2, Rehab A.; Rezk2, A.A. and Sofy3, A.R.*
...rees and occasionally in grapevine and mango. 'Ihe principal characteristics of the disease on sweet orange trees. It was incidence with 15.4, 4.5 and 1.5% respectively. HSVd was detected mainly in sweet orange trees and occasionally in apple, apricot, mandarin, grapevine, mango, peach, pear and plum trees with 25.2, 2.2, 7.2, 10.5, 12.4, 15.7, 65.6, 40.5 and 5.7% respectively. The principal characteristics of the disease on...
Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.
...ed in naturally infected grapevine by biological indexing and molecular methods. In the present study, direct tissue-printing and dot-blot on the membranes, procedures has been applied on a large scale for an initial screening of HSVd, CEVd and PSTVd in Egypt. The results showed that the tissue print assays allowed clear discrimination between healthy and viroid-infected grapevine plants than dot-blot hybridization, the numb...
El-Dougdoug, Kh.A. t , S.A. Ghaza12 , A.A. Mousa2, H. Fahmyj and A.R. sofy2
Sahar, A. Youssef t ; M.M.A. Al-Dhaher 2 and A.A. Shalaby
Abdul Haseeb1, Yasir Iftikhar1, Muhammad Ahmad Zeshan1*, Safdar Ali2, Rana Binyamin3, Salman Ghuffar4 and Muhammad Usman Ghani5,6
..., feutral’s early, grape fruit and sweet lime, both in healthy and declining orchards. Whitehead and Hemming tray method was used for the isolation of T. semipenetrans which showed that the population was low in declining orchards as compared to the population densities found in healthy orchards. Kinnow was the most infested by nematode with mean population 1161 while sweet orange was the least infested with 595 nematodes, in all localities. Maximum nema...
Bushra AL-Khaqani, Amira Mohammed*
...al plants, red wine, and grapes, has been researched for its potential anti-inflammatory, and antioxidant qualities, and to induce relaxation of airway smooth muscle. The current study investigated whether Resveratrol (RES) could lessen allergic asthma that ovalbumin (OVA) causes in rats. Current results revealed that RES improved significantly (P<0.05) allergic asthma attenuation by decreasing the number of infiltrated mononuclear cells in Bronchoalveolar ...
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