Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
... Although studies on the genetic diversity of the antigenically important RVA VP7- and VP4- protein encoding genes are important for vaccine development or judging the efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic diversity. Whole genomic analy...
Yanina Panzera1*, Nicolas Sarute1, Lucia Carrau1, Jaime Aldaz2, Ruben Perez1
...detailed analysis of the genetic diversity, evolution, and epidemiology of the virus in several countries. Our study allowed us to establish the presence of four genetic lineages in South America, being the continent with more lineages described to date, underscoring the high genetic diversity of the circulating strains....
Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5
...n rely upon knowledge of genetic diversity between species’ population. Two species were studied: Glossina palpalis palpalis and Glossina morsitans submorsitans using two mitochondrial DNA fragment primers- Cytochrome oxidase subunit II (COII) and Cytochrome b (CytB). Twelve samples of laboratory-reared population of each species were used for the study. Sequencing data were used to calculate haplotype, haplotype diversity and nucleotide di...
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
...nificance for preserving genetic diversity. The aim of this study was to reveal the genotype profile of indigenous types for TGC breed. In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatin gene CAST were studied. In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) o...
Atta ur Rehman1, Sher Ali2 and Sajid Malik2*
...gene differentiation and genetic diversity were estimated to be 0.01, 0.019 and 0.515, respectively. There were the highest affinities between the samples obtained from Lakki Marwat and Tank districts, whereas the least affinities were evident between Karak and South Waziristan Agency populations. Collectively, these results show the presence of sub-populations in lower KPK which may suggest a disturbed nature of these populations. Further studies are needed t...
Fukuan Du1,2, Yan Li2, Zhixin Wen1, Ruguo Chen1 and Pao Xu2*
... following investigating genetic diversity, constructing genetic maps, and marker assisted selection....
Waheed Anwar*, Muhammad S. Haider, Ahmad A. Shahid, Hamid Mushtaq, Usman Hameed, Muhammad Zia Ur Rehman and Muhammad Javed Iqbal
Guang-Xin E1, Yong-Ju Zhao1, Zhong-Yuan Zhang1, Yan-Guo Han1, Wei-Wei Ni1, Ming-Xing Chu2, Yue-Hui Ma2, Jian-Ning He3, Huai-Zhi Jiang4, Jia-Hua Zhang1, Zhong-Quan Zhao1, Yan Zeng1, Ri-Su Na1, Gao-Fu Wang5, Hang-Xing Ren5 and Yong-Fu Huang1*
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...d to study the molecular genetic diversity in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand the molecular signaling events during compatible plant nematode interactions by identifying the genes during the transition from preparasitic to parasitic stage in ear cockle nematode and molya ...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
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Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,*
...In order to evaluate the genetic diversity and population structure for the conservation and management of sables (Martes zibellina), we developed six microsatellite markers using a method of fast isolation by AFLP of sequences containing repeats (FIASCO) from them in this study. The primers designed by Primer Premier 5.0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged fro...
Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem
...fimicola, to examine genetic diversity. Strains were taken from two contrasting environments (Natural and Harsh) from the south facing and north facing slopes of Evolution Canyon, Israel. Sequenced amplicons were introduced to the NCBI database as a query in BLASTN. The query revealed >100% resemblance with the Ascomycota genus Sordaria and the topmost species in this investigation was fimicola. Circular Phylogram showed close relatedness ...
Syed Asif Imran Shah1*, Shah Jehan Khan2, Kalim Ullah1 and Obaid Ullah Sayal2
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...was planned to study the genetic diversity in the M. tuberculosis isolates isolated from clinical samples. In the current study total 794 patients were tested for suspected tuberculosis by fluorescence microscopy and GeneXpert system during the study period (2015-2016) at the Provincial TB Reference Laboratory, Khyber Pakhtunkhwa, Pakistan. Among the 794 patients studied, 170 samples gave positive results. Fifty-two Mycobacterium tuberculosis com...
Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan*
... to 1.00. Due to greater genetic diversity in Ocimum germplasm and its suitability for commercial cultivation even in the area under small holdings, the investigation suggest its genetic as well as biochemical investigation for the production of commercial varieties and exploitation of the plant for economic benefits of the local communities....
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...m the two rivers and the genetic diversity of wild population, 11 microsatellite loci were firstly used to evaluate the genetic characterization using 120 samples from 2 tributaries (ZZ and LX populations) of the Weihe river and 2 tributaries (YX and TB population) of Hanjiang river. A total of 59 alleles were found and the number of alleles in each locus ranged from 3 to 8, with an average of 5.4. There were 6 highly polymorphic loci and 4 moderately polymorp...
Mubashir Ahmad Khan*, Muhammad Yasin Mirza*, Muhammad Amjad*, Nazakat Nawaz*, Malik Shah Nawaz* and Doulat Baig*
ASSESSMENT OF GENETIC DIVERSITY IN GERMPLASM OF LINSEED ( L.)
...the existence of greater genetic diversity in the accessions for these traits. Dendrogram based on Euclidean distance coefficient using 10 quantitative traits, grouped all the linseed accessions into 13 clusters. Cluster II was the biggest and had 33 accessions followed by Cluster I having 11 accessions. For the development of high yielding varieties, best performing accessions of Clusters I and II could be used in hybridization programme by crossing with acce...
Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****
GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY
...LCuD). For estimation of genetic diversity regarding CLCuD tolerance, fiber quality and some yield contributing traits, 101 cotton genotypes imported from USA were evaluated. Different statistical procedures like cluster, principle components (PC) and correlation analysis were employed to identify the suitable genotypes that can be further exploited in breeding programme. Significant associations were found between yield contributing trait, boll weight and fib...
Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari*
ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...ure leaves to estimate 1 genetic diversity and hybrid identification. Out of the 26 RAPD primers used for DNA amplification, 14 primers produced polymorphic bands, while 12 produced monomorphic bands. These 14 primers yielded total 96 RAPD markers, out of which only 40 were found polymorphic which resulted in 41.66% polymorphism among the genotypes studied. The number of RAPD markers produced ranged from 4 to 12. Average number of 6.40 bands per primer was obs...
Muhammad Mohsin Raza*, Muhammad Aslam Khan*, Irfan Ahmad**, Ali Ahsan Bajwa***, Hafiz Muhammad Usman Aslam*, Badar Ahsan Ullah****, and Kashif Riaz*
FOREST PATHOGENS AND DISEASES UNDER CHANGING CLIMATE -A REVIEW
..., risk rating, planning, genetic diversity and facilitated migration, genetic protection and breeding for disease resistance and relating results to forest policy, planning as well as decision making, the suspicions innate to climate change effects can be minimized....
Zeqin Fu, Yunfang Tian, Yingying Ye*, Pengzhi Qi and Changwen Wu
...o choose for researching genetic diversity and population structure and provide more in-depth understanding of Mytilus coruscus.
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Tanveer Hussain1,*, Masroor Ellahi Babar1, Marcos De Donato2, Abdul Wajid1, Asif Nadeem3, Zahoor Ahmad3, Waqas Ahmad Khan4, Sunday O. Peters5 and Ikhide G. Imumorin6
...nderstand the origin and genetic diversity of native cattle breeds (Bos indicus) in Pakistan, partial sequencing of the mitochondrial gene cytochrome b (339 bp) was done in 136 individuals from 10 different breeds. Analysis of the Cyt b gene showed high conservation in Pakistani cattle as expected, with only 26 individuals showing nucleotide changes. Only 5 point mutations were present in multiple individuals (SNP), but one was specific for indicine cat...
Wang-Dui Basang1, Tian-Wu An2, Luo-Bu Danjiu3, Yan-Bin Zhu1, Shi-Cheng He3, Xiao-Lin Luo2, Wei-Wei Ni4, Xiao Wang4, Shu-Zhu Cheng4, Jian Wang4 and Guang-Xin E4,*
... the Naqu yak carry high genetic diversity but also indicates that frequent genetic material exchanges have led to smaller differences in genetic divergence between different ecotypes.
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Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
...We assessed the level of genetic diversity and genetic isolation of different langur populations using Randomly Amplified Polymorphic DNA (RAPD) genetic markers. We collected 86 different samples (feces 64, hair 13, blood 5, tissues 4) from 5 geographic langur populations of Pakistan and Azad Jammu and Kashmir (AJK) and succeeded in extraction of DNA from 23 samples, which were used for further genetic analysis. RAPD makers (n=8) produced 245 bands (30.62&plus...
Ai Guo1,2,3, Jiaguang Xiao4, Binbin Shan4, Tianxiang Gao5 and Yongdong Zhou3,*
...To better understand the genetic diversity and phylogeny of Coilia, the complete mitochondrial genomes of two lineages in Coilia mystus were compared. They were all typical circular double stranded DNA molecule with 17075 bp in C. mystus N and 16964 bp in C. mystus S, respectively, containing the standard metazoan set of 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and non-coding regions. The mitogenomes of ...
Muhammad Amir Maqbool1*, Muhammad Aslam1, Waseem Akbar2, Muhammad Waheed Anwar3 and Ehtisham Shakeel Khokhar1
Yuan Li1, Liyan Zhang2, Karhoe Loh3, Ji Feng1, Xinqing Zheng1, Puqing Song1 and Longshan Lin1,*
... China and Malaysia. The genetic diversity of all P. minor populations was moderate, and two major haplotype lineages were detected that were differentiated approximately 0.3 million years ago. These two haplotype lineages differed significantly in frequency distribution of Chinese and Malaysian populations, showing an imperfect geographical pedigree structure. Results of AMOVA also showed that the genetic differentiation was mainly among populations. A...
Fehmeeda Fatima, Asif Nadeem* and Maryam Javed
...enced (1344 bp) to study genetic diversity and evolutionary characteristics of Sindh ibex. In this research, samples of Sindh ibex were collected from Kirthar National Park, Sindh, Pakistan, the gene of interest was amplified, PCR products were sequenced and data was analyzed. The results of genetic diversity indicated that Parsimony informative sites were found to be 11 while Singleton variable sites were not detected. The ...
Aiguo Zhou1,3,4, Shaolin Xie1,4, Zhenlu Wang1, Lanfen Fan1, Yanfeng Chen2, QiaoYe1, Fang Zeng1 and Jixing Zou1,*
...ze the relationships and genetic diversity of two color morphs of northern snakehead. For the Chnannidae family, the average genetic distances of Cyt b gene was 0.171 (ranged 0.000 to 0.248) with the inter-species genetic distances. The intra-species genetic distance ranged from 0.000 to 0.002. For the haplotypes of two color morphs of Channa argus, the mean pair-wise genetic distances were estimated as 0.002, which were within the intra-species ...
Mubashir Ahmad Khan*, Nazakat Nawaz, Muhammad Arshad, Muhammad Ayub Khan, Tahira, Doulat Baig, Ihsan Ullah Khan and Shamim ul Sibtain Shah
...here was a wide range of genetic diversity in this set of sesame germplasm which could be utilized for crop improvement. It is further recommended that for making improvement in sesame crop, identified superior sesame lines viz. SG-115, SG-103, SG-78, SG-79, SG-31, SG-117, SG-108, SG-41 and SG-1 could be used in breeding program and further by making selections in segregating generations. This will help to increase sesame crop yield in the country. ...
Muhamad Jawad*, Shahid Riaz Malik, Muhammad Aqeel Sarwar, Muhammad Asadullah, Israr Hussain and Rabia Khalid
...onducted to evaluate the genetic diversity among 36 lentil genotypes for mechanized harvesting. Analysis of variance for qualitative and quantitative traits showed significant variability among the genotypes. Correlation analysis indicates negative correlation of yield with lowest pod height and highly significant correlation between lowest pod height and plant height. Cluster analysis performed for qualitative and quantitative traits separately. It was observ...
Liyan Zhang1,2, Zhidong Zhou1,3, Haiping Li1,2, Yanlong Qiao4, 5 and Yueping Zhang1,3,*
...t waters. The results of genetic diversity and genetic structure analysis of S. japonicus showed that these four populations exhibited moderate genetic diversity across the sampled geographical range of the species. However, no phylogenetic structure corresponding to geographic location was observed from the neighbor-joining (NJ) tree and haplotype network analyses, and gene flow and exact P test results reveal...
Zhen-Yang Wu1, Guang-Xin E2,*, Hui Ran1, Da-Hui Wang1 and Tian-You Yang1
...tions in Tongren had low genetic diversity and little genetic flow, which has led to substantial differences in genetic divergence between western and eastern populations. These results show that geographical isolation, especially by mountains, has played an important role in limiting rice frog migration.
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Tazeen Jamil, Saba Ijaz, Rabia Arif*, Faiza Akram and Muhammad Saleem
... an inevitable source of genetic diversity due to their abundance in eukaryotic genomes and their insertion in coding regions leads to altered gene expression. We examined the diversity of retroelements in four consecutive generations of different strains of Sordaria fimicola. The parental strains werecollected from two contrasting environments of Evolution Canyon i.e. harsh South facing slope and neutral North facing slope. Retroelements profile...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
...eeds implied the loss of genetic diversity and emphasized the need for conservation efforts for the Pakistani indigenous horse genetic resources....
Zhaochao Deng1, Na Song2, Yongzhen Li3, Tianxiang Gao1 and Zhiqiang Han1,*
...he genetic structure and genetic diversity of L. olivaceus, a 495 bp segment of mitochondrial DNA control region was sequenced. We collected a total of 65 individuals from three archipelagos (Xisha, Zhongsha and Nansha) in the South China Sea and 107 polymorphic sites were obtained defining 39 haplotypes. Based on the NJ tree, two distinct lineages were detected, with strong frequency differences in the geographical distribution. Both lineages were foun...
Jie Zhang1,2,*, Baradi Waryani3,4 and Qihai Zhou2,*
...t for Tibet Plantae. The genetic diversity and molecular invasive mechanism are urgently needed for both native and invasive populations. Present investigation details 12 polymorphic microsatellite loci and 12 to 26 alleles per locus denoted. Whereas, d expected and observed heterozygosity values ranged from 0.902 to 0.972 and 0.750 to 0.958, respectively. Moreover, the polymorphic information content (PIC) values ranged from 0.869 to 0.949. The cross-species ...
Yan-Bin Zhu1, Wang-Dui Basang1, Zhan-Dui Pingcuo1, Yang-Ji Cidan1, Sang Luo1, Dun-Zhu Luosang1, Yang-La Dawa1 and Guang-Xin E2,3,*
...ent study was to perform genetic diversity and population structure estimation on 382 individuals from seven Chinese Tibetan ecotype yak population using twenty-one microsatellites. The results revealed that the HO ranged from 0.4854±0.0194 in NNV to 0.6086±0.0267 in YRY, and the NA ranged from 3.86±1.98 in XMY to 6.05±3.37 in NNV. The least number of markers which deviated from Hardy-Weinberg equilibrium within the population was W...
Yacheng Hu1,2, Xueqing Liu1,2, Jing Yang1,2, Kan Xiao1,2, Binzhong Wang1,2 and Hejun Du1,2,*
...useful tool to study the genetic diversity of Dabry’s sturgeon.
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Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
...results also showed high genetic diversity and a certain degree of heterozygote deficiency of natural swimming crab populations. The advancing technology and its unique advantages will make molecular markers as novel and highly-efficient approaches for assessment of stock enhancement.
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