Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*
...ulating the secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) at puberty. The deficiency in release or action of GnRH leads to hypogonadotropic hypogonadism (HH) characterized by low FSH, LH and testosterone (T) and absent or impaired sexual development at puberty. Amongst others, mutations in GPR54 and GnRH receptor (GNRHR) are possible causes of HH. This study aimed at identification of mutations in GPR54 and GNRHR genes and their ...
Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1
...py. Numerous degenerated follicles with cytoplasmic vacuolization, mitotic catastrophe in mitochondria , chromatin condensation, mitochondrial vesiculation and dispersion at ooplasm were observed. In mitochondria, mitotic catastrophe , vesiculation, swelling and loss of organization of cristae were detected.Here we showed that TiO2 exposure trigger paraptotic type cell death in zebrafish ovary.
...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...d lymphocytic depletion, follicle necrosis, leukocytic infiltration, cyst formation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
...
Guang-Xin E1, Yong-Ju Zhao1, Yue-Hui Ma2, Ming-Xing Chu2, Jia-Hua Zhang1, Zhong-Quan Zhao1, Hui-Jiang Gao2, Huai-Zhi Jiang3, Di Liu4, Li Liu5, Yan-Bin Zhu6, Wang-Dui Basang6, Luo-Bu Danjiu7, Tian-Wu An8, Xiao-Lin Luo8, Shi-Cheng He7 and Yong-Fu Huang1,*
...dominant and nondominant follicles of goats. These data will contribute to research on the molecular mechanisms of dominant follicle selection in goats. In this study, 90276370 and 115579236 clear reads in dominant and nondominant follicles of goat were generated through Illumina paired-end sequencing, and their mapping rate was 84.99% and 84.47%, respectively. A total of 12577 differentia...
Abd El-Nasser Ahmed Mohammed*
...addition, visible antral follicles and corpora lutea was demonstrated on the ovarian tissues upon transplantation. Histological examination indicated that transplanted young ovarian tissues restored function better than elder ones. It could be concluded that unilateral ovarian transplantation partially restored their developmental competence for producing ova. Such result might be helpful in assisted reproductive studies for treatment of infertility and conser...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...he pre and postovulatory follicles (POF) of the freshwater teleost, Clarias gariepinus were observed by light microscopy. The germinal vesicle and the cytoplasmic organelles of the oocyte were disintegrated. The theca of atretic mature oocytes was hypertrophied and persisted to form the interstitial cells, whereas the granulosa cells were regressed and disappeared. Erythrocytes and leukocytes were also detected at the advance stage of atresia. Apoptosis...
Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1
Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
..., disappearance of lymph follicles and interfollicular edema
were the prominent histopathological changes observed in challenged group. It is
concluded that fish oil improve the growth performance and immune response in
broilers chicken against avian influenza virus infection....
were the prominent histopathological changes observed in challenged group. It is
concluded that fish oil improve the growth performance and immune response in
broilers chicken against avian influenza virus infection....
A.M. Abdul Azeem, A.N. El Shahat* and Ashraf M. Mounir
... of luteinizing hormone, follicle stimulating hormone and testosterone in addition to marked improvement in the level of total antioxidant capacity and glutathione content and the activity of superoxide dismutase activity and catalase activity of the liver and testes of lead-intoxicated rats. In conclusion GPE could ameliorate the damage and apoptotic effects occurred by exposure to toxic dose of lead.
...
Zhen-li Li1,2, Hui-fang Zhou2,*, Bo-ru Zhou2, Bei Liu2, Xiao-fei Jiang2 and Jian-ya Xu2
...vention. Then, the serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were detected by ELISA, FSHβ and LHβ expression levels in the pituitary were determined by qPCR, and GnRHR expression levels were assessed by qPCR and western blot analysis. Simultaneously, transcription factors, such as c-Jun, Elk-1, Egr-1, Nur77, CREB, and transduction molecules in PKC-MAPK, Ca2+-CAM, and cAMP-PKA signaling pathways, such as PKC, p38mapk...
Abid Hussain Shahzad1, Abdul Sattar1,*, Nasim Ahmad1, Ijaz Ahmad2, Deniz Nak3 and Yavuz Nak3
...d d90 post AI. Ovulatory follicle diameter was measured at timed AI and progesterone profile (ng/mL) on d30 and d60 post AI. Pregnancy rate was analyzed by Chi-square procedure while ovulatory follicle diameter and Progesterone profile by one way ANOVA (α=0.05). Ovulatory follicle diameter (Mean±SEM) was 15.19±0.17 (OVP0), 15.30±0.21 (OVP5) and 15.24±0.19 ...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...of middle diameter sized follicles than that of small and large sized follicles. BMP15 gene expression enhanced until morula stage but it fell sharply at blastula stage. Immunohistochemistry exhibited BMP15 protein was located in germ cells of testis, in primordial granulosa cells, primary, secondary, and antral follicles of ovary, and none in theca cells. The more conspicuou...
Khayyam1*, Muhammad Zahid1, Naiz Ali2 and Qadeem Khan3
..., 0.025respectively) but follicle stimulating hormone was significantly low (p=0.0007) in female HT patients. Follicle stimulating hormone and Luteinizing hormone concentrations were higher than normal range in both male and female Grave’s disease patients. Prolactin and serum gonadotropin showed fluctuations in Hashimoto’s thyroiditis patients but in Grave’s disease patients only the gonadotropins s...
Yan Zhou1,2, Hai Xia Han1,2, Qiu Xia Lei1,2, Jin Bo Gao1,2, Wei Liu1,2, Fu Wei Li1,2, Jie Liu1,2 and Ding Guo Cao1,2*
...nd in ovary, 6 mm - 8 mm follicles and 4 mm follicles from H2H2 chickens, and significant difference compared with those of H3H3 chickens (P<0.01). These findings suggest that VLDLR could be considered a candidate gene for egg production in chickens.
... 
Amit Sharma1* and Pankaj Sood2
Sonographic Assessment of Ovarian Follicular Dynamics during Breeding and Non-Breeding Season in Gaddi Goats
...±0.12), number of follicles at wave emergence during second (2.85±0.26 versus 1.77±0.14; P<0.01) and third wave (2.57±0.2 versus 2.0±0.16; P<0.05), along with shorter persistence of dominant follicle (DF, 9.25±0.45 versus 12.02±0.44, P<0.001) and early attainment of DF during first three waves was observed during B compared to the NB season. Whereas, significantly hig...
Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3
Muhammad Younis1, Muhammad Irfan-ur-Rehman Khan1*, Mustansar Abbas1, Ali Murtaza1, Imran Mohsin2, Muhammad Shahzad3 and Muhammad Zahid Tahir1
...In 3-wave cycles (n=14), follicles (≤ 3mm) emerged on Days 0.7, 5.2 and 10.5. In 4-wave cycles (n=2), follicles emerged on Days 0.1, 4, 8.5, and 11.5. The maximum diameter of preovulatory follicle and corpus luteum was 5.4±0.3 and 10.4±0.3mm, respectively. Regardless of the wave pattern, single ovulation occurred in each cycle. CL was first detectable on Day 4.0±0.1...
Imran Tarique1*, Muhammad Ghiasuddin Shah1, Illahi Bux Kalhoro1,
Zaheer Ahmed Nizamani2, Mansoor Tariq 2, Jamil Ahmed Gandahi1,
Saqib Ali Fazlani3 and Benazir Sahito3
...nd sweat gland, and hair follicle) was well developed and distributed in papillary and reticular layer in adult age group, while in young age group mainly observed at papillary dermal layer of all body regions. Sweat gland was mainly baggy-tubular shaped based on L/D ratio 7.64 to 8.3 range in all body skin regions, contain higher volume (3.2±0.1 μ 3 ×10−6 and 3.9±0.1 μ 3 ×10−6) in dewlap and thigh body region of adu...
Khlood G. Abdelkhalek*, Aly B. A. Badawy, Mohamed Fathi, Elshymaa A. Abdelnaby
...ions in plasma levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol 17β (E2), testosterone (T), and serum nitric oxide metabolites (NOMs) were measured in 5 Baladi bucks weighting (13–15 kg; with a body condition score of 3.5 ± 0.01) from 4-months- to 10-months-old in relation to testicular hemodynamics. Testicular morphometry, scrotal circumference, Doppler examinations, image analysis, and blood sampling were pe...
Xiaoyun He1, Yongfu La2, Jinxin Wang1, Ran Di1, Qiuyue Liu1, Xiangyu Wang1, Wenping Hu1 and Mingxing Chu1*
...function of transmitting follicle-stimulating growth biological information in animal breeding activities. In this study, we analyzed the structure of FSHR gene’s promoter, expression regulation and the correlation between polymorphism with fecundity. The results show that it is highly possible that the promoter of FSHR gene is in the region of 2045-2295bp and 2038-2288bp, TATA box was also found there. The expression differences were found...
Nacira Djabali1*, Souad Dougat2, Wahida Haddadi2 and Lamis Fadhli2
...inizing hormone (LH) and follicle stimulating hormone (FSH), the level of testosterone, the biological characteristics of the sperm, the weight and the histopathology of the testes were studied. The results obtained show a significant dose-dependent decrease in LH, FSH and testosterone in the treated groups compared to control. The administered solvent caused severe deterioration in sperm quality, decreased sperm concentration, mobility and vitality, with incr...
Asmaa S. Mohammed*, Ahmed F. Abou-Elnaga, Ahmed I. Ateya, Mohammed M. Fouda, Ragab A. Darwish, Usama A. Abou-Ismail
...nd expression pattern of follicle stimulating hormone receptor (FSHR) and estrogen receptor alpha (ER-α) genes in female rabbits. A total of 84 Nulliparous Blanc de Bouscat does were randomly allocated into one of four experimental groups (non-UVB, 12-h UVB, 8-h UVB and 4-h UVB) according to the duration of exposure to UVB radiation (n = 21 for each group). Behavioural patterns of rabbits were monitored for six consecutive weeks whereas, reproductiv...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3
...bryo ...
Aiman Al-Mufarji3, Abd El-Nasser Ahmed Mohammed3*, Rashid Al-Zeidi1, Haitham Al-Masruri2, Al-Hassan Mohammed4
... number of variable size follicles, as well as the number and diameter of CL were increased due to M. oleifera supplementation. It could be concluded that M. oleifera supplementation (50.0 and 100.0 g/kg diet) from eight weeks pre-partum to eight weeks post-partum of Naimi ewes improved the negative effects of the transition period of pregnant ewes.
Keywords | M. oleifera, milk, body weight, follicle
Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6
...ar alteration in growing follicle are important components. COD has been diagnosed by animal behavioral changes, nymphomania, anestrus, repeat breeding, pelvic ligament relaxation, tails head elevation, determination of progesterone level in plasma and milk by using kits of progesterone assay, and to confirm the diagnosis of COD, mostly trans rectal palpation and trans rectal ultrasonography methods have been used. COD should be treated by using different trea...
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...;0.05) whereas estrogen, follicle-stimulating hormone and luteinizing hormone decreased significantly (p<0.05) in the non-vaccinated group compared to vaccinated and control groups in both seasons. The study outcomes shown that climate has neither significant effect on reproductive physiology nor exaggerated the effect on an experimentally infected animal. Furthermore, it is concluded that pneumonic mannheimiosis causes a negative impact on reproductive per...
Ali Basim Abd Al-Hadi*, Tahreer Mohammed Al-Thuwaini, Mohammed Baqur Sahib Al-Shuhaib
...y higher (P≤0.05) and follicle-stimulating hormone (FSH) levels were lower in the CC genotype (85.10 ± 3.11) (pg/ml), (8.82 ± 0.52) (ng/ml), and (13.95 ± 2.71) (ng/ml) respectively compared to a CG and GG genotypes in twins versus singleton gestations. In conclusion, FST gene polymorphism significantly influences reproductive hormone levels in Awassi ewes. Excluding sheep that carry the GG genotypes of FST gene polymorphism suggests bei...
Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1
...upted growing and mature follicles were a distinctive microscopic feature in infected animals while such changes were absent in negative cows. The intact oocytes were clearly pronounced only in the negative animals. The fallopian tubes are free from any pathological lesions in the negative animals. Immunohistochemical, BVDV antigen was detected in ear notch, ovaries, fallopian tubes and uterus of one cow (1/30) (PI animal). Whereas, BVDV antigen was detected ...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Al-Hassan Mohammed4*
... oocyte from the ovarian follicle. Rodent oocyte maturation lasts 15-17hr. whereas that of ruminant oocyte lasts 24hr. Several factors affect on oocytes maturation either in vitro or in vivo and therefore affect development of the resulting embryos. Because of the significance of oocyte maturation stage on further embryonic development, this review aims to discuss the knowledge of oocyte maturation and its effect on further development of embryos in vivo and i...
Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid
...align: justify;">Ovarian follicles develop through several distinct phases during fetal and postnatal periods and they release their matured ova upon puberty. A finite number of primordial follicles form in the fetal ovary from primordial germ cells (PG) during the first stage of fetal development. The primordial follicles consist of oocytes surrounded by a single layer of pregranulosa fol...
N. Wachida1*, P. M. Dawuda2,1, I. U. Ate3,1, P. I. Rekwot4, 2
...ronmental heat stress on follicle diameter, and ovarian hormones concentrations. Twelve (n=12) non-pregnant, Zebu cows with 2-3 successive calving record were utilized. Temperature humidity index (THI) determined and rectal temperatures of cows were monitored. Cows were synchronized for oestrus using PGF2α. Follicular studies were carried out. Serum oestradiol and luteinizing hormones were monitored by Enzyme-Linked Immunosorbent Assay (ELISA). Blood sam...
Abhishek Pandit1, Suman Poudel2, Manish Gautam3* and Shambhu Shah4
...nd inhibits the dominant follicle maturation due to the suppression of both FSH and LH. Gonadotropin-releasing hormone or an analog is administered with PGF2α to estrous- cyclic and noncyclic cattle disrupts the patterns of follicular growth, inducing ovulation on a large follicle. To select and successfully implement the estrus synchronization regime in animals’ proper knowledge of the hormonal profile and funct...
Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana
...f exon 9 and intron 9 of follicle stimulating hormone receptor (FSHR) gene in Pesisir cattle to identify single nucleotide polymorphisms (SNP). The samples used in this study were blood samples (n= 70) collected from Pesisir cattle (aged 2-5 years). DNA was isolated and the targeted region was amplified using the polymerase chain reaction (PCR) technique. Then the amplified products were sequenced. The results showed that the population of Pesisir cattle used ...
Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4
...ent of testosterone (T), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were detected. The fasting blood glucose, serum insulin and insulin sensitive index changes were analyzed. The RT-PCR method was used to detect ovarian tissue TNF alpha and the level of IL-6 gene expression; Western blot was used to detect the changes in the expression levels of IKKβ and NF-κB protein expression. The results of fasting blood glucose and serum in...
Widiastuti Ardiansyah1,3, Osfar Sjofjan2*, Eko Widodo2, Suyadi Suyadi2, Danung Nur Adli2
...ge white and small white follicles had a significant effect (p <0.05). In conclusion, increasing the level of curcumin produced a linear increase in the number of bioactive compounds and the reproductive organs of Mojosari ducks.
Keywords | Bioactive compound, Follicle, Mojosari Duck, Reproductive Organ, Turmeric
...
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
...E levels increased serum follicle-stimulating hormone, luteinizing hormone, testosterone, immunoglobulin, and antibody titer, while reduced lysozyme. All PEE levels improved antioxidants capacity, while reduced malondialdehyde in blood serum and in seminal plasma. Advanced motility, membrane integrity, livability and sperm viability were improved, but sperm abnormality, apoptosis and necrosis were reduced in cryopreserved semen by all PEE levels. Total antioxi...
Aiman Al-Mufarji, Shaker Al-Suwaiegh, Abd El-Nasser Mohammed*
...medium and large ovarian follicles were recoded postpartum on day 18, and corpora lutea (CL) were recorded on day 21. Collection of blood samples from ewes and lambs were performed at -8 weeks and -4 weeks pre-partum, parturition, +4weeks, and +8weeks postpartum. The blood samples were analyzed for blood and metabolic profiles, liver enzymes, and minerals. The results indicated that ewes suffered from thermal stress during the study and the stress was alleviat...
Thekra Fadel Saleh, Omar Younis Altaey*
...d higher levels of lymph follicles and B lymphocytes in the proximal portion compared to the middle and distal portions. The complex architecture suggests that caeca in Muscovy duck play a significant role in microbial digestion and immunological functionality and Insight into caecal morphology enhance bird’s productivity and health.
Keywords | Biochemical, Food security, Histology, Histochemistry, Histomorphometry, Muscovy...
Beenish Shahid1* and Muhammad Ijaz Khan2
...ere collected from 2-8mm follicles. A series of experiments were conducted to evaluate the effects of oestradiol (2 μg/ml), recombinant human follicle stimulating hormone (0.05 IU/ml), human chorionic gonadotrophin (2 IU/ml) and insulin (0.12 IU/ml) alone or together in different combinations at intervals of 8, 16 and 24 h of incubation period during in vitro maturation on buffalo oocyte. The supplementation of TCM-199 wi...
Sri Rahayu*, Muhaimin Rifa’i and Widodo
...riables regulate ovarian follicle growth from the preantral to the antral stages. Growth differentiation factor 9 (GDF9), which is secreted by oocytes, is a paracrine factor that plays a vital role in follicular development. We performed a computational biology analysis to determine the role of GFD9 in mammalian ovarian function. We constructed a three-dimensional model of GDF9 using I-TASSER and human GDF9 protein sequences obtained from UniProt. We also anal...
Qingjin Li1,2, Zhiyuan Sui1,2, Jihu Zhang1,2, Zhishuai Zhang1,2 and Feng Xing1,2*
...lopment and ovulation of follicles in the ovary....
Waqas Alam1, Sar Zamin Khan1 and Rifat Ullah Khan2*
...hemistry of the cells of follicle stimulating hormone (FSH), leutenizing hormone (LH) and growth hormone (GH). The results of the present study indicated that birds kept under cage had enhanced egg production, fertility, hatchability and lower mortality than the birds managed on floor....
Sajida Batool1, Mubeen Akhtar1, Shafaat Yar Khan1,2*
... of luteinizing hormone, follicle stimulating hormone and interleukin-3 in both groups. Two tailed, independent t-test and Pearson co-relation was applied to analyze hematological and biochemical parameters while anthropometric parameters like weight and height were compared as percentages. Results of current study showed that body mass index was significantly (P<0.05) elevated in PCOS patients when compared with BMI of control females. Number of successful...
Cut Intan Novita1,2, Kartini Eriani3, Amalia Sutriana4, Tongku Nizwan Siregar5,6*, Ni Wayan Karja7
...ptosis of granulosa cell follicles. Bovine pituitary has been proven as an effective substitute for the functions of FSH and LH in some studies related to superovulation due to its high content of both hormones. Therefore, this study aimed to determine the effect of bovine pituitary extract (BPE) supplementaion to vitrification media. A total of 18 ovaries were usedwhich was divided into two groups: the vitrification medium group without Bovine Pituitary Extra...
