Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...on using hemotoxylin and eosin (H&E) and Mason trichrome stains (MTC). Immunohistochemical investigations for the expression of the proapoptotic protein (Bax) and the expression of macrosialin cluster of differentiation (CD68). APAP elevated of most of the previously measured parameters and decreased GSH, SOD, and total protein levels. Liver sections of H&E demonstrated liver injury characterized by centrilobular hepatocellular necrosis, CD68, and Bax ...
G.E. Odo1*, E.J. Agwu1, N.I. Ossai2, C.O. Ezea3, J. Madu1 and V. Eneje2
...in neutrophil, basophil, eosinophil and monocytes throughout the duration of exposure and decrease in the lymphocytes . The results on gill and liver tissues sampled showed concentration and time dependent significant increase (p < 0.005) in the values of malondialdehyde, catalase, superoxide dismutase, reduced glutathione, alkaline phosphatase (ALP), alanine aminotransferase (AST) and aspartate aminotransferase (AST). Similarly, aluminium phosphide enhance...
Sezgi Arman* and Sema Isisag Ucuncu
...stained with hematoxylin eosin (H-E). Sections of anterior, mid and posterior intestinal segments were also treated with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet ...
Sezgi Arman* and Sema Isisag Ucuncu
...stained with hematoxylin eosin (H-E). Sections of anterior, mid and posterior intestinal segments were also treated with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet ...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...ount (TLC), neutrophils, eosinophils, basophils, lymphocytes, monocytes, red cell distribution width (RDW) and mean corpuscular volume (MCV) were increased significantly (P < 0.05) which indicated hemolytic anemia. While, serum biochemistry showed significant elevation in alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine which indicate renal and liver damage. Concentration of N...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...a nutrient agar (NA) and eosin methylene blue (EMB) viz. 1.8×103 and 1.56×103 (cfu ml-1) in gut contents whereas on skin only total bacterial count (0.36×103)was observed and no coliform bacteria were present while least CFU’s were counted in group 2 among skin 0.14×103 cfu ml-1 and gut 0.10×103 cfu ml-1 on nutrient agar. The resul...
Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq2
...3.15, respectively. MCH, eosinophils and monocytes showed significant difference. Hb, PCV, RBC, MCV MCHC, heterophils, basophils and lymphocytes values showed non-significant difference. Furthermore, some of the hematological parameters are altered by T. gondii but not all. In literature a little information is present regarding the hematological changes due to T.gondii in chickens of Cholistan, Bahwalpur, Pakistan.This is pilot study one of a kind and need fo...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...ratio was assessed using eosin nigrosin stain under 40x magnification, concentration was calculated by hemocytometer, membrane integrity was examined by HOST solution. Conception rate was determined by inseminating 20 buffaloes (10 control and 10 best treatment group). After initial examination semen sample having motility ≥ 70% and morphology live dead ratio ≥ 80 % were diluted in Tris based extender consist of 0,2,4 and 6mM concentration of selenium. F...
Saddar Faheem1,2, Hameeda Kalhoro1, Naeem Tariq Narejo1*, Muhammad Hanif Chandio3, Memon Samina4, Shahnaz Rashid5 and Ghulam Abbas5*
...eutrophils, lymphocytes, eosinophil and monocytes did not show any significant difference in their counts of male and female fish during summer and winter season. Important serum constituents like protein, cholesterol and glucose were also assessed and it was reported that serum glucose was found more in male than female fish, while in male protein and cholesterol were high in female in summer. In winter season, female fish showed low level of cholesterol, tho...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...kocytosis, heterophilia, eosinophilia, monocytosis, and lymphocytosis. Serum biochemistry showed marked elevation in aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. Histopathological examination demonstrated degenerative changes, necrosis haemorrhages, and sloughing off epithelial cells of broad folds of caeca, mild lymph...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
...mined by hematoxylin and eosin. The levels of cytokines in lung tissues were assessed by Immunohistochemistry staining and Quantitative real-time (qRT)-PCR assays were performed to explore the effects of QZZKF on the expression of surfactant protein A (SP-A), B7 homolog 3 protein (B7-H3), interleukin (IL)-12 and IL-13. The expression levels of B7-H3 and IL-13 were upregulated after MPP infection in the mouse model (P<0.05), while the expressions of SP-A and...
Amal Hamad1, Ashraf M. Abu-Seida2*, Faisal A. Torad2, Nahed S. Thabet3, Shabaan M. Gadallah1
... leukocytes, neutrophil, eosinophil, lymphocyte and monocyte counts, serum levels of total protein (TP), albumin, urea, creatinine, creatinine clearance and serum enzymatic activities of alanine transaminase (ALT) and aspartate transaminase (AST) along the whole experiment. Meloxicam induced no sedation, no behavioral changes, insignificant increase in heart rate, significant increase in systolic, diastolic and mean blood pressure and significant increase in r...
Muhammad Zahid Iqbal1, Aneela Zameer Durrani1, Jawaria Ali Khan1, Nisar Ahmad2, Muhammad Usman1,*, Abdul Jabbar1, Saba Usman3, Ahsan Anjum3, Muhammad Husnain1, Nadeem Raza1 and Anwar-ul-Haq4
...s (11.25 ± 3.62), eosinopenia (4.95 ± 2.14), thrombocytopenia (232.50 ± 211.74), and decreased hemoglobin (7.005 ± 0.90) and PCV levels (21.29 ± 2.28). Whereas in affected cows only low hemoglobin level (7.25 ± 1.18), decreased PCV (22.29 ± 3.34), eosinopenia (9.6 ± 5.49), and thrombocytopenia (365.25 ± 227.32) were statistically significant (p<0.05). As far a...
Alaa Jaheen*, Noha Salem, Mohamed El-sherif
... alteration was elevated eosinophils in eczema-affected horses. The oxidant-antioxidant status showed a significant increase in malondialdehyde with a significant decrease in total antioxidant capacity, serum zinc, and copper concentrations. No significant changes were detected in both protein and lipid profiles. In conclusion, equine eczema is a stressful allergic skin disease that alters hematological and oxidant/antioxidant status in the equine and disrupts...
Wei Cui1, Yanwei Du1, Lijuan Jiang1, Yan Wei1, Yuguo Li1, Wenfeng Zhang1* and Ling Zhang2*
...rved through hematoxylin-eosin staining, and the expression of related proteins was detected. The results showed that, after BHD intervention, the degree of apoptosis of PC12 cells injured by HG was significantly reduced, and the cell proliferation was significantly increased. In model group, blood glucose increased significantly, weight loss, oral glucose tolerance was abnormal, while BHD group reversed the above changes. Most nerve cells in BHD group had rel...
Wafaa M.A. Ghoneem*, Muhammad M. Shaklouf, Ali M. Ali and Mohammed H. Bakr
...s, monocytes, basophils, eosinophils and neutrophils were not affected by treatment. Culbac® significantly (P<0.05) decreased blood concentration of ALT, AST, cholesterol, triglyceride and urea compared with control. While, lymphocyte transformation and phagocytic index were increased by (18 and 23%) and (20 and 62 %), respectively for G2 and G3 compared with G1. Average daily milk yield was increased by 2.23 kg/d and 0.9 kg/d in G2 and G3, respectively...
Ida Bagus Oka Winaya1*, Anak Agung Ayu Mirah Adi1, Putu Henrywaesa Sudipa2, I Gusti Ketut Suarjana2
...cessed using hematoxylin-eosin dyes (HE). On histopathological examination, congestion, edema, mild infiltration of neutrophils and macrophages in the meningen, and ischemic neuronal pyknosis were found in the brain. In the synovial tissue, there is a heavy infiltration of neutrophils with few macrophages. Microbiological examination showed the growth of small round colonies, grayish-white, mucoid but hemolysis reaction was not clearly visible. On Gram stainin...
Yasmin M. M. Mahmoud*
...te blood cells count and eosinophils% were significantly higher in G1 and G2 comparing with the control diet. In conclusion, kids fed diet supplemented with dried chicory leave showed the best results especially that of 1% supplementation, concerning digestion coefficients, productive performance, blood parameters as well as hematological profile.
Keywords | Goats, Chicory, Digestibility, Productive performance, Blood
...
Shujaat Hussain1*, Muhammad Saqib1, Khurram Ashfaq1 and Zia ud Din Sindhu2
...unt (11.94±0.04), eosinophils% (3.10±0.20), basophils% (0.76±0.06), platelets count (103.2±0.56103µ/l), glucose (55.13±0.84mg/dl), albumin (4.25±0.01g/dl) and sodium (110.26±1.21mEq/L) were observed in infected camels. This investigation demonstrated significant differences in hematological and serum biochemical variables of infected and healthy camels. Moreover, to set a reference line of blood variables ...
Chemoprotective Effect of Daphnetin against Benzene Induced Leukemia Cancer via Alteration of CYP2E1
Junling Liu, Chen Sun, Qiao Yu, Yanzhi Liang, Shanshan Lin and Meng Tian*
... neutrophils, monocytes, eosinophils, monocytes and basophils, respectively. Daphnetin treated rats showed the increased level of p21 and p53 and reduced level of cyclins D1 and E. RT-PCR showed the up-regulated of mRNA expression of CYP2E1 of daphnetin treated group rats as compared to other groups. The current study showed the anti-leukemic effect of daphnetin and highlights the possibility of its use in leukemia to minimize the side effect of the usual ther...
Tertia Delia Nova1*, Yulia Yelita2, Rizky Machicula1
... monocytes, heterophils, eosinophils, and basophils. Three treatments of 5%, 10%, and 15% levels of kiambang flour in feed were ready. The variables measured were also ration consumption, weight gain and conversion as well as carcass, and abdominal fat during growth. The experimental results showed that the average feed consumption ranged from 4034.89 to 4207.40 g/head, weight gain was 710.22–889.84 g/head, and conversion was 4.8–5.9. The highest c...
Dhiya Altememy1, Mohammad Darvishi2*, Samira Shokri3, Saber Abbaszadeh4
... 14, and 21, hematoxylin-eosin slides (H&E) of the tissue, angiogenesis rate, and division of fibroblast cells and Masson’s trichrome slides were used to determine the rate of collagenization, wound, and extracellular matrix. After the treatment, the size of the wound in the Tio2 N.P. synthesis by Origanum vulgare, Tio2 N.P. synthesis by carvacrol, methicillin, Tio2 N.P., and alginate pad groups of diabetic rats and alginate pad group of non-diabetic...
Tlou Grace Manyelo1,2, Nthabiseng Amenda Sebola1, Jones Wilfred Ng’ambi2, Monnye Mabelebele1*
... heterophils, monocytes, eosinophils, and basophils compared to other treatments. At the age of 90 days, the chickens which were treated with 5, 10, and 15% ALM had higher (P < 0.05) heterophils, lymphocytes, eosinophils, and basophils than other treatments. However, 5% ALM had a higher (P < 0.05) nutrient digestibility in the Boschveld chickens. ALM inclusion did not affect the gastrointestinal tract, small intestine,...
Muhammad Mudassar Shahzad1*, Hamna Rashid1, Syed Makhdoom Hussain2, Sana Bashir1, Fatima Khalid1 and Nisar Ahmad3
...utrophil, lymphocyte and eosinophil) are higher in fish that were fed on control, 1, 3 and 5 g/kg of probiotics respectively. Based on these recordings it was concluded that probiotics supplementation at 2g/kg in an RSM-based diet was very helpful for maximum performance of C. catla fingerlings in contrast to the control and other test diets....
Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1
...(neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best concentration of sericin and curcumin was successfully achieved and was used in further studies. In the group that was given carrageenan alone, the number of erythrocytes, hemoglobin and platelets were decreased, whereas count of white blood cells was increased significantly. The effect of sericin and curcumin was dose depe...
Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1
...(neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best concentration of sericin and curcumin was successfully achieved and was used in further studies. In the group that was given carrageenan alone, the number of erythrocytes, hemoglobin and platelets were decreased, whereas count of white blood cells was increased significantly. The effect of sericin and curcumin was dose depe...
Ayma Aftab, Samia Afzal* and Muhammad Idrees
...D-dimer, serum ferritin, eosinophil sedimentation rate (ESR) test, tuberculosis test, real time PCR and high-resolution computed tomography (HRCT) were done to rule out the cause of flu like symptoms. HRCT reveals a haze area in the right perihilar region adjacent to medial part of horizontal fissure on the 3rd day of manifestation of symptoms. Radiological studies showed early consolidation of COVID-19 whereas RT-PCR showed negative results. Chest CT imaging ...
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
...meters were stained with eosin-nigrosine, intact plasma membrane was stained with Hypo-osmotic (HOS) solution, acrosome status was stained with peanut agglutinin (PNA), and DNA integrity was stained with acridine orange (AO). The results showed that the volume, pH, motility, abnormality, intact plasma membrane, and concentration of fresh semen of Bali and Simmental bull were not significantly different (P>0.05). Likewise, the frozen semen of Bali and Simmen...
Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5
...vealed severe multifocal eosinophilic dermatitis and inflammation of subcutaneous hypodermis (panniculitis) whereas, in the skin with high ivermectin residue, there was mild multifocal epithelial hyperplasia with mild perivascular mononuclear cells infiltration. Conclusion: Different levels of ivermectin residue were detected in bovine skin samples collected from slaughterhouses in Egypt however the degree of damage caused by ectoparasites exceeds the damage c...
Jawad Ahmad1, Huang Yun-He2, Arab Hussain1, Amjadullah1, Ahmad Yar1, Subhanullah1, Zeshan Ali1, Hamid Sattar3, Tariq Hussain3, Hamayun Khan3, Ali Gohar6, Obaidullah4, Nan Zhu Fang5, Yanya Fang5, Ihsan Ali3* and Zhongshu Li5,*
...igh in other groups, the eosinphil was high in group 3 where the monocytes was high in group 1 and group 3 as compared to other groups. The ESR was high in group 2 and low in group 4 as compare to other groups....
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...ntrast-phase microscope (eosin-nigrosin staining) was investigated. Moreover, superoxide dismutase (SOD) assay, a fatty acid composition with gas chromatography and lipid peroxidation TBARS (Thiobarbituric acid reactive substances) content were used for oxidative stress evaluation. Frozen-thawed results showed significant (p < 0.05) enhancement in all sperm parameters with 10 ng/ml of DHA supplementation. DHA improved (p < 0.05) SOD levels in all supplem...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
..., the viability with the eosin-negrosin staining method, and membrane integrity using the hypoosmotic swelling test (HOST) at the fresh semen, 5th, 24th, and 48th hours post-cold storage. The results revealed that in the 5th hour, percentage of motility, viability, and membrane integrity were not significantly different. However, at the 24th and 48th-hour results revealed significant differences by adding honey (P < 0,05). The 0.4% honey supplementation was...
Hussein Jabar Jasim1*, Amer Murhum Al-Amery2
...ell infiltration such as eosinophil, Kupffer cell and scattered lymphocytes in the area previously migrated by young flukes as well hepatocytes displayed a fatty change with dilated hepatic sinusoids. Furthermore, our findings show that the bile ducts have hyperplasia, which is associated with fibrous cholangitis and an abundance of inflammatory cells with accumulated bile pigment (hemosiderin) in the dilated bile ducts.
Keywords...
SAEED AHMED*1, ZIA-UR-RAHMAN1, SALMA KHALID2, TARIQ KHAN1, ARSHAD IQBAL3
& ZAMARUD SHAH4
Xiaowei Huang1, Chao Ma1, He Lin1, Yuchen Wang1, Yan Xu1, Guangfu Lv2* and Zhe Lin1*
Thekra Fadel Saleh, Omar Younis Altaey*
Tabark F Ahmed, Nibras Z. Yahya*
...onkey agar, chrome agar, eosin methylene blue agar, then VITEK® 2 System was performed Data showed the sample was positive as a result of E. coli O157. The second experiment examined the pharmacodynamics of ascorbic acid in various states, including alone and in combination with ciprofloxacin and erythromycin. Results showed that the MIC of ascorbic acid (AA), ciprofloxacin (CIP), and erythromycin (ERY) against E. coli O157 was 16 mg/ml, 2 g/ml, and 256 g/...
Zhipeng Song1,2, Jialiang Xin1,3, Xiaoli Wei1,2, Abula Zulipiya1,3, Kadier Kedireya1,2 and Xinmin Mao1,3*
...r 12 weeks. Haematoxylin-eosin (H&E) staining was used to detect the pathological changes of retinal tissue. Cell apoptosis was analysed by TUNEL staining. The expression of retinal proteins was detected by tissue immunofluorescence and immunohistochemical staining. The mRNA level of VEGF was detected by RT-qPCR. In the in vitro experiments, cell viability was detected by CCK-8 reagent. The expression of related proteins was detected by western blot. We fo...
Al Salihi karima Akool1* , Almas. M. Al-Bayati2, Iman Mousa Khaleel3
Promising Immunomodulation Potential of Ganoderma lucidium and Oregano as Mixture on Broiler Chicken
Fateh A. Badi*, Anwar A. G. Al-Kubati, Naif Al-Gabri
..., .03 respectively). The eosinophils were significantly increased in the GO group in comparing to G and C groups (p=.04, .05 respectively). G group result in significantly increase (p<.05) of lymphocytes count compared with other groups including the C group. While the O group showed significantly decreases in lymphocytes (p<0.05) compared with other groups. In Monocyte count the O group showed significantly (p<.05) increase the count comparing with...
Sajida Batool1, Mubeen Akhtar1, Shafaat Yar Khan1,2*
...utrophils, monocytes and eosinophils percentage and serum level of luteinizing hormone, follicle stimulating hormone and interleukin-3 in both groups. Two tailed, independent t-test and Pearson co-relation was applied to analyze hematological and biochemical parameters while anthropometric parameters like weight and height were compared as percentages. Results of current study showed that body mass index was significantly (P<0.05) elevated in PCOS patients ...
Ying Chen1, Chao-Zheng Li2, Zhun Yu3, Jia Zhou4, Yan Xu4, Zhe Lin4, He Lin4* and Xiao-Wei Huang4*
...y ELISA. Hematoxylin and eosin (HandE) staining was used to detect the histological changes of rat myocardium. Western blotting (WB) was used to detect the protein expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and NADH quinone oxidoreductase 1 (NQO1) in rat myocardial tissue. In the results, ECG showed that the MIRI model was successfully established; VAP can significantly reduce the activities of LDH, CK-MB ...
Maria Kikelomo Adegun1*, Femi Godwin Ekundayo1, David Daisi Ajayi2
...ed except lymphocyte and eosinophil. Lymphocyte was lowest (p<0.05) in T2 (23.33±1.53%) compared to similar (p>0.05) values obtained in the other treatments whereas eosinophil was highest (p<0.05) in T1 (3.00+0.00%) compared to the similar (p>0.05) values obtained in T2, T3, and T4. Additionally, the serum biochemical indices demonstrated typical immune reactions (p<0.05) to the test substances in total...
SHAGUFTA ANDLEEB1*, ASMATULLAH2, SHABANA SHAUKAT1 & SIDRA NAWAZ2
Wasan Ali Hasan1*, Manal Abdulkhaliq Ibrahim2
...lysis was conducted. The eosinophils, neutrophils, mononuclear cells, and total white blood cell count were all increased (P<0.05) in the positive control (B_group) when compared to the negative control group. Both D and E_groups, when treated with famotidine, showed a significant reduction (P<0.05) in the eosinophils, neutrophils, mononuclear cells, and total white blood cell count. Additionally, the histopathological...
