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Siyu Yang1, Fukuan Du2 and Pao Xu1,2*

...lia nasus) is a rare and endangered species and also an important resource with high economic value. Somatostatin (SS) is a neuropeptide family which effects growth, development and metabolism. In this study, full-length of one type of SS cDNA from C. nasus was synthesized, cloned and sequenced. This SS cDNA encodes a protein with 114 amino acids that contains the SS14 sequence at its C-terminus. This putative peptide is identical to that generated by the SS1 ...
Md. Yeamin Hossain1,*, Md. Alomgir Hossen1, Md. Nasir Uddin Pramanik1, Fairuz Nawer1, Md. Mosaddequr Rahman2, Suraiya Sharmin1, Dalia Khatun1, A. H. Bahkali3, Abdallah M. Elgorban3 and Khairun Yahya4
...ustify;">The threatened (endangered) carp, Botia dario (Hamilton, 1822), is an important, nutritionally valuable food fish in south Asian countries i.e., Bangladesh, Bhutan, India and Nepal.The present study describes the first complete, inclusive description on life-history traits of B. dario, including ─ sex ratio, length-frequency distributions (LFDs), length-weight relationships (LWRs), length-length relationships (LLRs), conditi...
Jie Wu1, Jiagui Zhu2, Ke Wang2, Dejing Cai2, Yingying Liu1, Yanzhen Bu1,* and Hongxing Niu1,*
...nippon)is a globally endangered bird. Aimed to save and restore the small population in Henan Province, China, a reintroduction program was conducted in the Dongzhai National Nature Reserve in 2007. Wild birds bred in semi-wilderness were reintroduced. Habitat selection and breeding ecology of crested ibis in Dongzhai National Nature Reserve has not been studied. To clarify the breeding status of the crested ibis and to provide more reference for the reint...
Liushuai Hua1,3, Shiping Gong1,*, Xiangjing Zhong2, Jun Tao2, Yu Chen2 and Jieming Deng2 
...non megacephalum) is endangered in the wild because of illegal trade, overharvesting and habitat destruction. Captive breeding is an effective protection against extinction, but safe overwintering is still technically difficult to captive big-headed turtles. Determining optimal hibernation conditions is important in resolving this problem. In this study, the effects of two important environmental conditions (water depth and availability of sand beds) on ch...
Minmin Chen1, Xiaoke Zhang1, Kexiong Wang2, Zhigang Liu1, An Wan3, Daoping Yu1,*, Hong Ji3 and Fangzhen Peng1
...lis) is a critically endangered species, as the whole population in the wild suffered sharp decline and fragmentation. Observations of the local spatial and temporal distribution dynamics can help establish better conservation strategies. In order to understand the characteristics of habitat chosen, a continuous 15 months observation on the distribution patterns of the Yangtze finless porpoise at the confluence of Yangtze and Wanhe rivers was conducted. Th...
Jie Wu1, Jiagui Zhu2, Ke Wang2, Dejing Cai2, Yingying Liu1, Yanzhen Bu1,* and Hongxing Niu1,*
...nippon)is a globally endangered bird. Aimed to save and restore the small population in Henan Province, China, a reintroduction program was conducted in the Dongzhai National Nature Reserve in 2007. Wild birds bred in semi-wilderness were reintroduced. Habitat selection and breeding ecology of crested ibis in Dongzhai National Nature Reserve has not been studied. To clarify the breeding status of the crested ibis and to provide more reference for the reint...
Muhammad Siraj-ud-Din1,2, Riaz Aziz Minhas1, Usman Ali3,*, Mayoor Khan2, Muhammad Siddique Awan1, Nuzhat Shafi1 and Basharat Ahmad1
...vignei vignei) is an endangered wild sheep still occurring in small pockets in certain areas of Gilgit Baltistan (GB) besides Ladakh in India. The present study was conducted to determine habitat use and feeding preference of urial in Gilgit Baltistan. Current study is the first and only detailed study about the habitat and feeding ecology of these wild sheep in Pakistan. The study provides baseline data about the study topic which will help conservation m...

 Mohammed Mahbub Iqbal*, Mehedi Hasan Kanon, Mohammad Amzad Hossain, Abul Hossain, Shamima Nasren, Md. Jahidul Islam, Md. Arifur Rahman

...ies 5 were vulnerable, 7 endangered, 1 critically endangered, 3 exotic, 20 not threatened and 1 not evaluated according to IUCN, Bangladesh, 2000. The Shannon-Weaver diversity index was found highest (3.12) in June and lowest (2.9) in January. Margalef richness index was found highest (3.02) in July and lowest (2.70) in December. On the other hand, Pielou’s evenness index was lowest (0.82) in three successive months i....
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

...ol for propagation of an endangered medicinal plant Neurada procumbens L. from Cholistan desert in Pakistan. Nodal segments from healthy grown plants were used as explants and cultured on standard Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best sh...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...ae) is an indigenous and endangered cold-water fish in Tsinling mountain, China, and only is distributed as spot in the Weihe River and Hanjiang River among Tsinling mountain, To access relationship of populations from the two rivers and the genetic diversity of wild population, 11 microsatellite loci were firstly used to evaluate the genetic characterization using 120 samples from 2 tributaries (ZZ and LX populations) of the Weihe river and 2 tributaries (YX ...

 Raja Zoq-ul-Arfeen*, Aamir Saleem*, Sarwat Naz Mirza*, Muhammad Akmal**, Hafiz Muhammad Tayyab* and Obaid Afzal***

BIODIVERSITY AND PHYTOSOCIOLOGICAL STUDIES OF UPSTREAM AND DOWNSTREAM RIPARIAN AREAS OF PAKISTAN: SPECIAL REFERENCE TO TAUNSA WILDLIFE SANCTUARY AND KETI SHAH FORESTS
...in future plantation and endangered species can be conserved.

...
Filiz Kutluyer* 
... fertilizing capacity in endangered trout Salmo coruhensis, Anatolian trout Salmo rizeensis and rainbow trout Oncorhynchus mykiss. Different activation media (NaCl, 0.3%; NaHCO3, 1%) were supplemented with L-tryptophan [Control (0), 0.5, 1, 2, 3, 4 and 5 mM] for assessing sperm motility and its duration, fertility and hatching rate of eggs. The results from the present study indicated that addition of L-tryptophan increased the ...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...pictus pictus) is an endangered species and captive populations are important for conservation. Canine distemper virus (CDV) and canine parvovirus (CPV) are two infections threatening the survival of this species. Based on a limited number of animals, live attenuated vaccines have been suggested to provide protective immunity in the African wild dog; however, their use has been hampered by the fear of vaccine-induced disease. Kolmården Wildlife Park ...
Liumeng Zheng1, Yanmei Wang1, Jiagui Zhu2, Ke Wang2, Dejing Cai2, Yuanzhao Qin3, Yuming Guo3, Hongxing Niu1,* and Yanzhen Bu1,*
... nippon) is globally endangered due to habitat loss and destruction. In 2007, a reintroduction project was implemented in Dongzhai National Nature Reserve, Henan Province, and 78 crested ibises were released between 2013 and 2015, with the aim of establishing a self-sustaining wild population outside the Qinling Mountains, where crested ibis was first reintroduced. The aim of this study was to detect suitable habitat areas, and evaluate the factors affecti...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
Wei Meng1,3, Tianyan Yang2,*, Yunguo Liu1, Mahmut Halik1 and Tianxiang Gao2
...othoracinaeis a rare and endangered aboriginal fish in Xinjiang. In this study, the complete mitochondrial genomes of G. dybowskii from Tarim River system (16, 677bp) and Ili River system (16, 667bp) were sequenced. Besides, their genetic characteristics were also identified and compared simultaneously. Genetic distance and sequence differentiation suggested that great genetic variation existed within species and the sample from Kaidu River in South Xin...
Peng Xu1, Bin Liu1,2, Yongqiang Zhao3, Shicheng Lv3 and Changhu Lu1,*
...n the population size of endangered birds,red-crowned crane. Then, an interpretation model with high deviance explained was explored. More and continuous attention should be paid to improve considerable measures of crane conservation based on climate change.
...
Mouslim Bara1,2,* and Luciano N. Segura3
...i>Aythya nyroca) and endangered White-headed Duck(Oxyura leucocephala). Our results highlight the importance of preserving the Garaet Hadj Tahar as a water bird refuge. 
...
Abderraouf Chouaib Rebbah1, Mohcen Menaa2,*, Salah Telailia3,Menouar Saheb1 and Mohamed Cherif Maazi2
...tected species, only one endangered species, and five endemic species to the Maghreb and/or to North Africa. The presence of these species with patrimonial value reinforces the importance of the conservation of Sidi Reghis avifauna. Bird abundance, species richness and species diversity were significantly higher in pure pine woodlands than in mixed oak-pine and oak forests. According to PERMANOVA and ANOSIM tests, and the NMDS plot, the avian assemblages of S...

John Ogunsola1*, Richard E. Antia2, Benjamin O. Emikpe2 and Olajumoke A. Morenikeji

...animals especially those endangered. This report evaluates unaided, sporadic, captive breeding of zoo animals over a 10-year span. Clinical records of observed gestation or egg-laying, as well as successful or failed parturition or hatching of various species of captive animals were obtained. The animals included reptiles, amphibians, aves and mammals, with both sexes placed in the same enclosure with some degree of access to one another. Among mammals, surviv...

 Zhi Yijin and Shao Mingqin*

...ere ascertained for this endangered species. The Xiuhe, Fuhe and Raohe river systems exhibited large steady populations, with the maximum size ranging from 25 to 68 individuals for each river. Restoration and maintenance of suitable habitats for the scaly-sided merganser is imminent as the species is at risk of extinction due to its small population size. The total sex ratio of the merganser during the study period was 0.397 (n = 790) and the average sex ratio...
Iram Maqsood1,2,3 and Ke Rong *1,2
... hope to this world most endangered species and give a chance to be part of this ecosystem biodiversity in perspective future. China contributed greatly to conserve ecological and cultural roles of Chinese alligators and its sustainable utilization.

...
Guo-hai Wang1, Zai-xi Yang1, Pan Chen1, Wei-ning Tan2 and Chang-hu Lu1,*
... septentrionalis, an endangered tree species in a karst habitat in southwest China. Twenty-seven bird species were recorded feeding on its seeds, and fourteen bird species were confirmed as seeds dispersers. The chestnut bulbul (Hemixos castanonotus), striated yuhina (Yuhina castaniceps), scarlet minivet (Pericrocotus flammeus) were the main seed dispersers. The average seed dispersal distance was 10.69±4.97 m and 82% of the fir...
Muhammad Waseem1, Ahmed Raza1, Hamera Aisha1, Muhammad Naeem Awan1, Tariq Ahmad2, Rabia Nazir2 and Tariq Mahmood2,*
..., and has been listed as endangered since 2014 and also included in Appendix-I of CITES. The IUCN estimates indicate a decrease of ≥ 50% in the global Indian pangolin population over the next 21 years, highlighting the need for protecting the species against illegal trade. In the current study, we investigated the scale of its poaching and illegal trade in the country. Results revealed that poachers use spotlight and tracking techniques (42% respondents). S...
Muhammad Raza Khan1, Tariq Mahmood1,*, Hira Fatima1, Faraz Akrim2, Shaista Andleeb1 and Abdul Hamid1
Huanxin Zhang1,2, Hongshuo Tang3, Jun Chen1, Yu Zang1,2, Xuexi Tang1,2,* and Ying Wang1,2,*
... innate immunity of this endangered carnivore. 10 TLR genes (TLR1-10) were initially identified from the African hunting dog and lesser panda. The results showed that most of the TLR genes were very conservative in the African hunting dog. We found 6 of ten TLR genes had the evidence of positive selection, only a small proportion of sites show evidence of selection, ranging from 0 to 9. In the present study, only four and three common positive selection codons...
Anila Naz Soomro1*, Hiroshi Suzuki2, Sadaf Tabassum Qureshi3 and Wazir Ali Baloch1
...ine the life cycle of an endangered atyid C. sakishimensis and its co-existing species C. typus. Entire continuum of stream was divided into five stations (St.1, St. 2, St. 3, St. 4 and St. 5). Monthly sampling was carried out using a scoop net (1 × 1 mm) via the sweep method, sweeping efforts were counted. The results revealed that C. sakishiemnsis was restricted atSt. 1 only, a few juveniles of C. sakishiemnsis were recorded...
Dongping Liu1, Guogang Zhang1, Chao Wang2, Baoping Qing2 and Jun Lu1,*
...ction of this critically endangered species under a changing environment.
...
Waqas Ahmad Shams1*, Gauhar Rehman1, Khurshaid Khan1, Ibrar Ahmad1, Saima Bibi1, Saif Ul Islam2, Dawood Safi1, Saba Gul Ayaz1
...udy will help to prevent endangerednes of species.  
...
Minghao Gong1*, Shiliang Pang2, Zhongyan Gao2, Wanyu Wen1, Ling Zhang3, Gang Liu1, Huixin Li1, Fawen Qian4 and Wenfeng Wang2
...="font-size: small;">The endangered red-crowned crane (Grus japonensis, RCC) has the smallest population of all Chinese cranes, and is also susceptible to climate change because of its large body size, short migration distance, and specialized diet. We examined climate change-induced RCC habitat shifts and suggested some purposeful, resolute and crucial improvements needed to protect appropriate areas that ensure the RCC’s long-term survival. Base...
Maria Syafiqah Ghazali1, Azlan Che’ Amat2 and Nor Azlina Abdul Aziz1*
...pose a serious threat to endangered species. The present study was conducted to observe the occurrence of gastrointestinal parasites in large felines in a Malaysian zoo. Ten faecal samples were collected from pumas (Puma concolor, n = 5), African lions (Panthera leo, n = 3), a spotted leopard (Panthera pardus, n = 1), and a black panther (Panthera onca, n = 1). All faecal samples were examined for parasite eggs, larvae, and oocysts ...
Qin Liu1,2, Bin Wang1,3, Yu Xu1,4, Xiuyue Zhang1, Tao Zeng1* and Jianghong Ran1*

 

...lliformes species, is an endangered bird endemic to western China. Previous studies suggested that it had the behavior of facultative cooperative breeding, which was rarely reported in Galliformes. In this study, we isolated 17 tetra-nucleotide microsatellite loci to test parentage and kinship for a wild population with 29 individuals from 10 different families (A-J). The 17 loci with the mean polymorphic information content (PIC) of 0.566 and the combined pro...
Muhammad Umar1, Mubashar Hussain1*, Muhammad Faheem Malik1, Muhammad Naeem Awan2 and David C. Lee3
...unds for this critically endangered species. 
...
Muneer Abbas1*, Dilbar Hussain2, Muhammad Saleem2, Abdul Ghaffar2Sohail Abbas3, Niaz Hussain1 and Abdul Ghaffar1
...iptera: Tephritidae) has endangered the trade of Pakistani fruits in foreign markets. Use of IPM is best way to control fruit fly by avoiding harmful insecticidal spray application in order to save natural enemies and environment. Experiment was conducted in three guava, citrus and mango producing districts of Punjab i.e. Faisalabad, Sargodha, Toba Tek Singh with five treatments. Each experimental unit comprised of 1 ha area. Five treatments viz....
Md. Alal Hossen1, Mohammad Amzad Hossain1*, A.K.M. Munzurul Hasan1,2, Bipresh Das1, Sohel Mian1, Mohammed Mahbub Iqbal1
 
Tariq Mahmood1*, Luqman Ullah Khan1 and Muhammad Naeem2
...rea, which is critically endangered and is surviving at only a few places in the country. Species richness was high in winter season than in summer. Similarly, diversity index of avifauna was also high in winter season. The study concludes that Manglot Wildlife Park contains a diverse and rich avifauna, especially the population of white-rumped vulture that needs to be monitored regularly.
...
Hui-Ming Li1, Bi-Ze Yang1, Xue-Jun Jin1,2, Lin-Miao Li1, Hai-Ying Jiang1
Xiu-Juan Zhang1 and Jin-Ping Chen1*
...anis javanica) is an endangered mammal species exhibiting scales characteristics. The conservation and management of this species could benefit from a better understanding of its genetic diversity and structure. In this study, 24 novel SSRs were isolated from full-length transcriptome and they were used for assessing of parent-offspring relationship for M. javanica. All SSR markers were highly polymorphic with a mean of 6 alleles per locus, ranged f...
Aftab Raza Khan1, Azhar Abbas Khan2*, Javed Iqbal3, Arif Muhammad Khan4, Zeshan Hassan2, Hafiz Muhammad Aatif2 and Umbreen Shahzad2

Huda Bilal1, Hasnain Raza2*, Kaynat Ahmed2, Iqra Tariq2, Qurat-ul-Ain2, Sana Sarfaraz1, Sanaullah1, Maryam Maqsood3 and Ali Raza3

...re many species that are endangered and extinct. This review illustrates that climate change is a severe environmental concern that is affecting land (plants, soil, microbes, animals, and arthropods) and aquatic biodiversity (waterbodies, phytoplankton, coral reefs, and fishes) drastically.

...
Jialiang Han1, Guohou Liu1*, Wenke Bai2,3, Qixian Zou4, Ye Cao5, Caiquan Zhou2,3*, and Guy Michael Williams6
...cus francoisi) is an endangered primate that has experienced a decline in population size and associated loss in habitat as a result of human disturbance (IUCN, 2020). We investigated human disturbance and habitat use of François’ langur in Mayanghe Nature Reserve, Guizhou, China, by analyzing the effects of various types of human disturbance on habitat use using the spatial simultaneous autoregressive error model (SAR). We found that habitat ...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...s, and help conserve the endangered germs of hills.

...
Romaan Hayat Khattak1, Zhensheng Liu1*, Liwei Teng1* and Ejaz ur Rehman2
... The presence of several endangered species in many areas remains unveiled due to the lack of faunal inventories. In such situations, it is imperative to make proper records of the existing fauna on local scales to strengthen the conservation practices. Twenty-one species representing 18 genera, 12 families and six orders of mammals were recorded in Nowshera district, Khyber Pakhtunkhwa province (KP), Pakistan from an inventory conducted from November 2018 to ...

I Nyoman Sulabda1*, I Nengah Wandia2, I Ketut Puja3 

...tle is an indigenous and endangered cattle population in Taro village, Bali and only inhabiting restricted areas in forests of Taro village. Current population are 57 head. To provide basic information concerning allele and locus in BoLA gene, the BoLA microsatellite loci (DRB3 and BM1815) were used to evaluate allel diversity using 18 samples of Taro white cattle. Genetic variability was low, with observed heterozygosity and expected heterozygosity for locus...

Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*

...lation investigations in endangered species. Based on the whole genome sequences of Macaca thibetana, we successfully developed 26 SNP loci that were sensitive to non-invasive samples, then based on which, genetic diversity and population structure of three populations of M. thibetana were estimated. Our results showed the novel SNP loci were polymorphic and bi-allelic. The observed and expected heterozygosity across 38 individuals varied from 0.184-0.605 and ...

Aamir Ibrahim1, Bingyao Chen1, Hassan Ali2, Imran Ali3, Yang Cao1 and Guang Yang1*

...(Platanista minor) is an endangered species found in the Indus River system of Pakistan including Beas River in India which is a part of Indus River system, enlisted in Appendix I of CITES Red List of threatened species. Currently, the whole population across the Indus River in Pakistan is divided into four subpopulations. Although photo-identification efforts on freshwater dolphins were successfully made on the Irrawady dolphin (Orcaella brevirostris) in Sout...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
...ases and conservation of endangered mammalian species. Practically, current reproductive biotechnologies are species-specific because of differences in estrous cycle, seasonality, structural anatomy, gamete physiology and site for semen deposition or method of embryo transfer. Therefore, this review was conducted to collect and consolidate the current knowledge of assisted reproductive techniques of males and females in addition to their importance for oocyte ...

Omnia M. Khattab1, Morcos I. Yanni2, Hala K. Abdelmegeed2, Mahmoud Eliwa1, Naglaa M. Hagag1, Sara M. Elnomrosy1

...arnivores including many endangered animals. Regular appearance of the virus increases the importance of evaluating the percentage of identity in relation to vaccine commercially used, detecting sequence changes related to virus virulence or tropism. In this study Fifty-seven fecal samples were collected from suspected cases of cats. 19 fecal samples were positive using Rapid FLV Ag test kits kit (VDRG FLV/FIV Ab rapid kit). Fifty-seven blood samples of the sa...

Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5

...conservation of multiple endangered plant species.

...

Yong Bin Zhao1, Hui Song1, Guo Gong Yi1*, Xi Lian Hao1, Bai Wang2, Zhi Wen Chen2

...feather samples. As some endangered birds cannot be captured, feather sampling is the only feasible method for isotopic research. However, whether it can be used for research on individuals remains to be clarified. In this study, the values of δ13C and δ15N of the rachises and barbules of 27 feathers from 3 pigeons were measured, and cluster analysis of these samples was carried out by using the system clustering method. The results showed a signif...

Yingying Wang, Jianyi Yu and Lichen Zhou*

...th China tiger, the most endangered tiger subspecies endemic to China, remain only a little more than 200 today, was susceptibly infected with Toxoplasma gondii. In this study, we used polymerase chain reaction to detect DNA, and enzyme-linked immunosorbent assay to test for T. gondii antibodies. Antibodies (S/P>0.31) to T. gondii were found in 20 (26.32%) of the 76 tigers, while all blood samples tested through nested PCR were negative. This is the first i...

Wen Xiong1*, Shengao Chen2, Dong Xie3, Qiang Wang4, Yanxia Li5, Lei Pan6, Xiaolei Ren7*, Wei Tang8*, Kang Chen9 and Ross N. Cuthbert10

...4 species are classed as endangered by the IUCN. We identify and discuss habitat loss, pollution, sand mining, over-fishing and non-native species as the major threats to freshwater fish biodiversity in this system. Thus, we propose that protected areas and bans on fish introductions, should be adopted and better enforced in this and other freshwater systems.

...

Adeel Kazam1, Safdar Sidra2, Zulfiqar Ali1*, Rida Ahmad1,3, Ahmad Bilal1 and Aliza Batool1,3

...rcus macrourus), and one endangered species steppe eagle (Aquila nipalensis) were observed during the study. These species require special attention for conservation as illegal hunting is one of the major threats to the species in the study area. Other threats include fishing, use of insecticides and pesticides.

...
Yongtao Xu1, Dandan Wang1, Xiaolong Hu1, Minling Li1, Ming Tang1, Wuhua Liu2, Jianwen Zhan2 and Weiwei Zhang1*
...whole is thriving, it is endangered and locally extinct in many areas of China, and the South China sika deer (Cervus nippon kopschi) is one of three subspecies left in China. Diet analysis is one of the core contents in studying animal habitat requirements, and a study of their diet could provide valuable reference for species conservation and management. The metabarcoding trnL regions of the chloroplast genome from 30 fecal samples of Taohongling sika deer w...

Pakistan Journal of Zoology

April

Pakistan J. Zool., Vol. 56, Iss. 2, pp. 503-1000

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