Siyu Yang1, Fukuan Du2 and Pao Xu1,2*

...vel in the brain. During embryogenesis, SS1 mRNA was detected during early-stage embryonic development, decreased during subsequent developmental stages then increased gradually from the stage of midgastrula onward. This study provides some basic evidence that SS1 may play a role in growth, development and metabolism in C. nasus, and provides a basis for further study of SS neuropeptide family in C. nasus.

...

Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2

... the development of SCNT embryos in the BMI.

...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
... virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate at the rate of 0.1ml of EID50 (virus titer 105.50/100μl) on 2nd week of age through eye drops. Group B specific antibody negative (SAN) chicks were challenged ...
Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...atogenic effects in mice embryos.
...
Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
...ation was carried out on embryonated chicken eggs. Identification of H protein was undertaken through hem agglutination Inhibition (HI) test while N typing was done using reverse transcriptase polymerase chain reaction (RT-PCR) procedure. Out of 600 examined locations twenty two (22) H9N2 subtypes isolates were recorded. The total prevalence recorded was 3.66%and out of 498 broiler operations yielded 17 (3.4%) positive isolates and 44 gol...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

Ayşe Gül Harlıoğlu*
...n, fertilized eggs, eyed embryo, yolk-sac larvae and absorbed yolk-sac larvae were investigated in rainbow trout(O. mykiss). The polyunsaturated fatty acids (PUFA) were found the highest followed by monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) in the unfertilized eggs. On the other hand, PUFA was the highest followed by SFA and MUFA in the semen. The proportions of the some fatty acids, such as C14:0, C16:1n-7, C18:0, C18:1n-9, C18...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...essful fertilization and embryonic development. Different techniques have been used to determine the integrity of DNA in which acridine orange (AO) assay is easy and less costly. Though used in many species but the variability exists in technique. Therefore, the present study was aimed to optimize the steps of AO staining protocol for buffalo sperm which has not been explored yet. Semen was collected from two mature buffalo bulls, diluted in tris citrate egg y...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali

...erent parameters such as embryo physiological age, co-cultivation period, addition of L-cysteine in co-cultivation medium, heat shock to immature embryos prior to infection, resting stage and Agrobacterium strains that deliver T-DNA into plant cell from which whole plant can be regenerated. The results indicated that optimal embryo physiological age is 15 and 18 days after pollination and ...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali* 

... of raising the immature embryos as a starting material for genetic transformation. Unlike immature embryos, the development of coleoptilar node is seasonal free and green house independent which would accelerate the work relevant to genetic transformation of maize. MS medium fortified with 3 mgL-1 BAP and 5 mgL-1 picloram produced maize seedlings with expanded coleoptilar nodes. Longitudinal cut sections of expanded coleopt...
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
...sion levels in the early embryonic development, relatively high expression levels in the 1st day and 5th day of the pupa stage, and highest expression levels in the molting stage. What’s more, BmARM-like protein could be detected by immunofluorescence in all analyzed tissues, including head, midgut, hemolymph, fat body, testis and ovary. More importantly, the relatively high expression level of BmARM-like mRNA were observed i...
Karim El-Sabrout* and Mohamed H. Khalil
...ity (period length, dead embryos and percentage) and hatch chick performance (weight, vitality). The experiment was carried out in two groups with total number of 1800 eggs (900 eggs for each group). Eggs were incubated 12 h of light (natural) then 12 h of complete darkness (G1); and 12 h of light (natural) then 12 h of LED lighting (G2). From the obtained results, there were no significant effects of complement LED lighting” after effect on hatchability...
Teresa Napiórkowska* and Julita Templin
...first ten days of spider embryogenesis.
...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
...stently expressed during embryogenesis. These results indicated that CcSoxD plays an important role in the development of the nervous system and may be involved in sexual development in carp. And they provide a foundation for further study of the function of CcSoxD genes during carp development and neurogenesis.
...
Justyna Batkowska1,*, Lukasz Wlazlo2, Kamil Drabik1, Bozena Nowakowicz-Debek2, Karrar I.A. Al-Shammari3 and Magdalena Gryzinska1
...e infertile eggs or dead embryos, as well as samples of eggs to microbial analysis were. After 17.5 days of incubation its results were evaluated. Chicks were reared for 14 days and their livability and body weight gains were registered. The effectiveness of grapefruit juice as a disinfectant for hatching eggs was noticed in terms of reducing the development of microflora on the eggshell. Its use did not directly affect the hatching results and chick quality, ...

 Md. Nazirul Islam*, A. K. Azad**, Leon O. Namuco***, Teresita H. Borromeo***, Maria Lourdes O. Cedo*** and Edna A. Aguilar***

MORPHOMETRIC CHARACTERIZATION AND DIVERSITY ANALYSIS OF A MAKAPUNO COCONUT POPULATION IN U.P. LOS BANOS
...bsp;Genetic diversity of embryo cultured makapuno coconut whose endosperm is thicker and softer instead of hard crispy, growing at the University of the Philippines, Los Baños, was assessed using morphometric characters from March 2004 to October 2005. Shannon 2 Weaver Diversity Index and Chi-square (÷ ) tests were used to analyze variation in both the qualitative and quantitative characters. Substantial variation in crown shape, bole category, c...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...in was isolated from SPF embryonated chicken eggs. Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis reveal...
Shagufta Andleeb*1, Shabana Shaukat1, Chaman Ara2
... groups. Chicks and dead embryos were studied for morphological and morphometric analysis. Liver tissues were dissected out from each group for histological analysis. Cadmium chloride induced significant developmental abnormalities such as reduced body weight and crown rump length, exencephaly, ablepharia, crossed beak, gastroschisis, crooked toes, non-alcoholic fatty liver and pyknosis. Fresh garlic juice has successfully attenuated these toxicities and incre...
Shagufta Andleeb*1, Fariha Zahid1, Noor-ul-Ain1, Yousra Nisar1, Chaman Ara2
...tudy, teratogenicity and embryotoxicity of permethrin was tested in developing chick(Gallus domesticus). Different concentrations of permethrin (0, 5, 10 and 20ppm) were prepared in sterilized distilled water. These doses were injected into eggs on 3rd day while recovery of embryos was done on 7th day of incubation. Disrupted embryos with microcephly, hydroceph...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...maturation and early embryonic development after in vitro fertilization. Sheep follicular fluid (SFF) was collected from different ovaries and supplemented to the in vitro maturation (IVM) media with proportions 0%, 10%, 20% and 40%. Oocyte maturation was assessed in different treatments and then cumulus-oocyte complexes were used for in vitro fertilization. Relative quantitative expression of mRNA transcripts related to apoptosis (Bax...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...he isolation of virus by embryonated egg inoculation technique. Moreover, out of 507 samples, 479 serum samples were scrutinized by enzyme linked immunosorbent essay (ELISA). Sero-prevalence of AIV among different species of wild birds was as follows; peacock (n=35; 14%), duck (n=5; 2%), migratory water fowl (n=3; 1%), pheasant (n=2; 0.8%), grey leg goose (n=1; 0.4%), turkey (n=1; 0.4%), eagle (n=1; 0.4%) and crane (n=1; 0.4%), for domesticated bird species se...
Tengfei Lu1, Wenhua Pei1, Shuang Zhang2, Yangnan Wu1, Fenghao Chen2, Xiao Han2 and Weijun Guan1,*
...ues, as they do with the embryonic stem cells. However, a good many experimental tissues and organs were obtained from model animals but rarely from livestock. In this study, AMSCs were obtained from aborted sheep fetuses (1-3 month) under sterile conditions. Primary AMSCs were sub-cultured to passage 25 in vitro. The gene of Oct-4, Rex-1, CD29, CD44, CD71, and CD73 were identified by RT-PCR and immunofluorescence technique. The results showed that they...
Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*
...aginal junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells. The immunocytochemistry results showed that neurexophilin 1 was expressed in both the epithelial cells of chicken genital tract and DF-1 cells. Immunofluorescence revealed that neurexophilin 1 was expressed in cytoplasm as well as cell nucleus of chicken fibroblasts and Hela cells. Western-blot analysis displayed neurexophilin 1 in the cell and cell culture fluid of DF-1 cells and Hela ce...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
...al defects in developing embryo.
...

Madeeha Arshad, Asmatullah, Chaman Ara

Embryotoxic effects of sodium arsenate in Mus musculus

Akshay Sharma*, Mohit Mahajan, Madhumeet Singh and Pravesh Kumar 

...o judge the viability of embryo (up to 42 days), fetus (after 42 days) and late embryonic mortality, if any. Sexing of the twins was done on 56th and 70th day post artificial insemination and two male twins were confirmed on the basis of genital tubercle, median raphe, scrotal and penis impression. With the aid of ultrasonography and color Doppler technique, it is possible to determine the twins’ zygosity and viability...
Mirza Imran Shahzad1, Hina Ashraf2,*, Muhammad Arshad3, Sabeeha Parveen4, Amna Aslam4, Nargis Naz4, Zahid Kamran1, Sumbul Gohar Khalid5, Sajid Hameed1, Muhammad Ashfaq6 and Muhammad Mukhtar7

 

...in 7-11 days old chicken embryonated eggs. The viral loads were determined through heamagglutination (HA) test. The methanolic extract of each plant was found effective against NDV but in varying order. The active extracts were further used in different concentrations and IC50 of each extract was calculated. The extract of Achyranthes aspera was found most effective with IC50 3.125μg and the extract of Oxystelma esculentum

Rizwan Ullah Shah* and Iqbal Munir 

...l medium through somatic embryogenesis (cotyledon and hypocotyl) for callus induction followed by shoot and root regeneration of Brassica carinata. Seeds were sterilized and germinated, of which one week cotyledon and hypocotyl were used for callus formation and shoot regeneration at five different level of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l), and root regeneration at f...
Osman Ergene1,*, Isfendiyar Darbaz1, Serkan Sayiner2 and Selim Aslan1
...nfirmed and 9 cows whose embryonic mortality (EM) was determined. In PAG-Serum, there was a statistically significant difference (P <0.05, P <0.01) between the measurements on 40th day when EM was detected and on the 28th and 32nd days of pregnancy. In the PAG-Milk and PSPB-serum tests, there was a difference (P <0.05) in terms of blood values between the 32nd day and the 40th day when EM was detected. Statistically significant d...

 Xu Lijie, Obaid Ullah, Liu Haixing, Ihsan Ali, Zhongshu Li* and Nan-Zhu Fang*

...ammalian preimplantation embryos. Peroxisome proliferators-activated receptor gamma (PPARγ) functions in the nuclear regulatory factor 2 (Nrf2) antioxidant pathways by binding to the promoters of antioxidant genes and regulates the expression of genes that associate with NADPH oxidase. To understand the role of PPARγ in the development of early embryos and define the mechanism responsible for the arrest in develo...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*

 

...tal efficiency of cloned embryos from somatic cells remains low, and many factors contribute to overall development of the embryo, including gene expression by the donor cells. The human hematopoietic stem/progenitor cell 117 (HSPC117) protein has been identified as being involved in placental formation, and can be modified by epigenetics. However, whether HSPC117 affects development of cloned
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
... from microopetations on embryo development, we analyzed 60 the spent culture media of porcine parthenogenetic embryos experienced different types of microopetation using Raman spectroscopy. The results showed that Group II with the puncture of egg zona and plasma membrane had the highest cleavage rate and blastocyst rate of porcine parthenogenetic embryos, which was significantly higher t...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...ent of Nili Ravi buffalo embryos. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium al...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
... in the whole process of embryogenesis and folliculogenesis, early high level and then down regulated. It was significantly expressed higher level in COCs of middle diameter sized follicles than that of small and large sized follicles. BMP15 gene expression enhanced until morula stage but it fell sharply at blastula stage. Immunohistochemistry exhibited BMP15 protein was located in germ cells of testis, in primordial granulosa cells, primary, sec...
Beenish Zahid*1,2,7, Javed Iqbal Qazi2, Amir Zohaib2, Asim Aslam3, Raheela Akhter3, Haleema Sadia4, Qurat ul Ain5, Razia Sultana6, Irfan Irshad3 and Sobia Alyas7
...e suspected samples into embryonated eggs of 9-11 days. Isolated virus was confirmed through haemagglutination and haemagglutination inhibition test. Virus isolation rate of NDV from cloacal swab was 12% (6/50) and from oropharygeal swab was 8% (4/50). The 238-bp region of F gene of NDV was amplified by using specific primers of reverse transcriptase-polymerase chain reaction (RT-PCR). Out of 50 field samples 11 cloacal swabs (34%), were positive and out of 50...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
... on meiosis progression, embryo developmental competence and DNA double-strand breaks (DSBs) in mouse oocytes and resultant early embryos. Germinal vesicle (GV) oocytes were first cultured to monitor the progression of germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) during in vitro maturation (IVM), then the harvested metaphase II (MII) oocytes were parthenogenetically activated to evaluate pronuclear...
Sara Zafar* and Asmatullah
...e harm to the developing embryo and Pomegranate juice significantly reduced the detrimental effects caused by atenolol.
...

Naseem Zahra1* and Shajia Jabeen

...husk. In Brown rice, the embryo may or may not be left undamaged depending upon the hulling process. Brown rice may become white rice when the bran layer is exposed of in the milling process. In present era where diseases are common and people are in search of nutritious diet containing minerals and essential compounds, brown rice is found to be healthiest and minerals rich food commodity. Due to its appearance, color and texture it is rarely used. Brown has a...

Pakistan Journal of Agricultural Research

June

Vol. 33, Iss. 2, Pages 192-421

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