Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
E-mail | Tomas.Jinnerot@sva.se
...ratory disease (CIRD) in dogs. Other disease agents include various respiratory viruses and co-infection can exacerbate the clinical signs. CIRD is highly contagious and can spread rapidly, especially among dogs in close quarters. Fast and accurate diagnostics enables the selection of proper management. Our objective was to develop a sensitive and specific real-time PCR for detection of B. bronchisept...
Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...d tested against Meloidogyne hapla both in-vitro and in-vivo. All fungi proved to be efficient in reducing final population of northern root knot nematode M. hapla and giving vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L. muscarium proved to be more ...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...root knot nematode, Meloidogyne incognita, and the nematophagous fungus, Lecanicillium muscarium, on tomato. The nematode population densities were measured by determining the number of galls and egg masses, juveniles and eggs per root system, and reproduction factor. Four initial populations (Pi) (500, 1000, 1500, and 2000 eggs) mixed with antagonistic fungal conidia levels (1×103, 1×104, 1×105, 1×106) were used during the experiments....
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...root knot nematode, Meloidogyne hapla, through soil application under greenhouse conditions. A significant (P=0.05) reduction in the number of galls and egg masses, eggs and juveniles (J2), and the reproduction factor (Rf=Pf/Pi) was observed in the plants treated with L. muscarium compared to the control. However, all chemical nematicides were also effective in reducing the nematode infestation significantly (P=0.05) in soil compared to the control, but there ...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...or cells when produced endogenously in the cells or applied exogenously. Ceramidases, which are main component of ceramide pathway catalyze metabolism of proapoptotic ceramide to anti-apoptotic sphingosine and other fatty acids. In this study we have evaluated the pro-apoptotic effect of ceranib-2, a human ceramidase inhibitor and of a short-chain C2 ceramide on human lung adenocarcinoma cells, A549 by determining cytotoxicity of these agents by trypan blue as...
Glomus mosseae (Gerd and Trappe) and Neemex Reduce Invasion and Development of Meloidogyne incognita
Atta ur Rehman Khan1,2*, Nazir Javed2, Shahbaz Talib Sahi2, Tariq Mukhtar1, Sajid Aleem Khan2 and Waqas Ashraf3
...d development of Meloidogyne incognita was tested in eggplant roots in greenhouse pot trials. Neemex (5 g, 10 g and 15 g) and G. mosseae (100 g, 150 g and 200 g) were applied as protective treatment. The roots of eggplant were inoculated with 1000 second stage juveniles of M. incognita. Eggplants inoculated with nematodes only served as control. Each treatment was replicated tenfold. Data were recorded after one week interval up to five we...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
... of rabies in humans and dogs living in Lahore, Pakistan.
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Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6
...t knot nematode (Meloidogyne incognita) infects passion fruit (Passiflora edulis) in Mizoram, India and infected plants showed declined symptoms and poor fruit yield. Infested plants displayed stunting and yellowing and thick-root symptoms. The association of the root knot nematode species was identified based on observations on morphology of different life stages, beta-esterase phenotype and amplification of rDNA and partial sequence homology of...
Urooj Fatima1, Arslan Mehboob2, Muhammad Abid3, Muti-ur-rehman Khan4 and Tahir Yaqub4*
...gnificant threats to the dog population, around the globe. To ascertain the current situation and genetics of CPVs in Pakistan, a total of fifty samples from sick dogs suspected for parvovirus infection were collected from different geographic areas of the country. Samples were screened for positivity and verified for CPV using PCR. From a 30% positive samples for hemagglutination, nineteen (38.4%) samples were positive in P...
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...ext-align: justify;">Pseudogenes were previously regarded as molecular fossils, non-functional by-products of genome evolution. However, it has been indicated by several lines of evidences that some pseudogenes are active. Using current data of NCBI we have retrieved 65 pseudogenes from the genome sequence of human pathogenic bacteria Helicobacter pylori (H. pylori) strain 26...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...root knot nematode, Meloidogyne incognita and wilt fungus, Fusarium oxysporum f. sp. lycopersici on tomato in laboratory and pot culture experiment. All the isolates were isolated by serial dilution and cultured in liquid protein supplemented broth medium and culture filtrates (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual culture of wilt fungus with...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...nging to the genus Meloiodogyne. Further, molecular markers like RAPDs, AFLP, SSRs and PCR–RFLP of rDNA have been employed to study the molecular genetic diversity in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand the molecular signaling events during compatible plant nematode int...
Jia-xiang Wang1,2,* and Peng Li1,2
...justify;">Ghrelin, the endogenous ligand for the growth hormone secretagogue receptor, has been identified in the pancreas of many vertebrates. While ghrelin has been found in the cerebellum and gastrointestinal tract of African ostrich chicks, little is known about its distribution in the pancreas ofthe African ostrich. In the present study, the distribution and morphological characteristics of ghrelin-immunopositive cells in the African ostrich pancreas were...
Adnan Yousaf1,Jia Wu1, Qaiser Shakeel2, Yasir Iftikhar3, Muhammad Irfan Ullah4, Uzma Tahira5,Mustansar Mubeen1 and Wubei Dong1,*
...ultivars against Meloidogyne incognita and Sclerotium rolfsii and their integrated management by using derosol, cadusafos and Trichoderma harzianum in the green house at 25 ± 4ºC. Primarily, 15 chilies cultivars viz., 28-2010, C-72, Sanam, C-73, Gola Peshawari, 27-2010, Tata Puri, C-302, C-33, C-19, 5-2010, C-68, 11-2010, 18-2010 and 15-2010 were evaluated against M. incognita and S. rolfsii. Two wee...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...Among nematodes, Meloidogyne incognita (Kofoid and White) chitwood showed the maximum frequency percentage (72.3%). Results confirmed the presence of fungi and nematodes interacting each other to enhance the disease. Paecilomyces lilacinus (Thom) and Trichoderma harzianum (Rifai) (alone and in combination) were used as bio-control agents against isolated pathogens at three different concentration viz., R, R/2 and R/4. Combination of bio ag...
LX Ye1,2, JX Wang1,2,*, P Li1,2 and XT Zhang1,2
.../div>...
Xiaoting Zhang1,2, Jiaxiang Wang1,2,*, Peng Li1,2,* and Lixun Ye1,2
.../div>...
Senol Celik1,* and Orhan Yilmaz2
.../div>...
S. Shahid Shaukat*, Mian Sayed**, Aly Khan, M. Azhar Khan* and Babar Qazi***
...ulus reniformis and Meloidogyne incognita had similar population density levels and were more or less equally suppressed by all the treatments in the order: Carbofuran > Tridax procumbens > Fertinemakil Plus > Withania somnifera. The yield of guar was only slightly increased but not significantly....
Aly Khan* and S. Shahid Shaukat**
...ominant species was Meloidogyne incognita. Species diversity (H’) was highest in Wadh while lowest in Ali Dasht. Equitability (J’) component of diversity was highest in Kork and lowest in Ali Dasht, while species richness (d’) component was highest in Alat. The soils associated with Pomegranate orchards were coarse-structured, alkaline with low maximum water holding capacity. In most cases similarities were low between localities....
Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2*
...ommon infection in young dogs and is caused chiefly by canine coronavirus (CCoV) and canine parvovirus (CPV). While, CPV is considered the most important cause of diarrhea, studies have shown an increasing prevalence and importance of CCoV around the world. Fatal CCoV infections have been described, however, there is limited information on the molecular diversity of CCoV in many parts of the world. In this study, canine fecal samples from diverse States of Bra...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...bs were collected from 6 dogs with severe respiratory syndrome at the Animal Clinics of Nanjing Agricultural University in 2014. One viral strain was isolated from SPF embryonated chicken eggs. Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of ge...
Muhammad Zameer Kayani1,*, Tariq Mukhtar2 and Muhammad Arshad Hussain3
...aging effects of Meloidogyne incognita have not been assessed on cucumber, therefore, the present studies report the effects of five initial population densities of M. incognita on growth and yield of cucumber and nematode reproduction. All inoculum densities caused significant reductions in growth and yield of cucumber and were found to be positively correlated with the latter. On the other hand, ages of plants at inoculation had negative correl...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...;
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Muhammad Iftikharul Husnain1, Aneel Salman1, Inayatullah Jan2*, Tahir Mahmood3
...trolling for potential endogeneity by using locality as an instrument. Unsurprisingly, the magnitude of temperature coefficient increases substantially when endogeneity is controlled and becomes significantly higher (6 percent to 13 percent) than the previously reported findings in the literature. The findings suggest that endogeneity is a crucial issue and must be controlled before carryi...
Shuai Shang1,2, Honghai Zhang2,*, Xiaoyang Wu2, Qinguo Wei2, Jun Chen1, Huanxin Zhang1, Huaming Zhong2 and Xuexi Tang1,*
...s2r genes in raccoon dog (Nyctereutes procyonoides) was still unknown. As a true omnivorous mammal, the Tas2r genes may play a critical role in food selection of raccoon dogs. Thus, we explored the evolution of Tas2r genes in raccoon dogs. In this study, Tas2r genes including eleven intact genes and four pseudogenes from r...
Ghulam Muhae-ud-Din1,4, Anam Moosa1, Umer Farooq Ghummen1, Muhammad Jabran1, Amjad Abbas1, Muhammad Naveed2, Abdul Jabbar1 and Muhammad Amjad Ali1,3,*
...ria. ...
Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1
...dal effects against Meloidogyne javanica. Anti-J2 antagonistic test divided the isolates into 5 antagonistic groups or AGs. AG1 was observed as the most antagonistic group, causing an average of 66.6% J2 mortality. Genetic variability among these isolates, was studied at molecular level by randomly amplified polymorphic DNA procedure. A selected set of three random primers was used for this purpose, which resulted in 113 bands on gel for the 15 isolates of T. ...
Tariq Mukhtar
...valuated against Meloidogyne incognita on tomato. The application of T. harzianum and T. viride significantly increased shoot weight and decreased root weight of tomato in a dose dependent manner. Doses of 8×103 and 1×104 cfu/g of soil showed maximum increase in shoot weight and decrease in root weight. On the other hand, both the antagonistic fungi caused significant reductions in number of galls, egg mas...
Muhammad Zameer Kayani1,*and Tariq Mukhtar2
...ive potential of Meloidogyne incognita on different cucumber cultivars commonly cultivated in Pakistan, therefore, the objective of the present study was to evaluate the reproductivity of M. incognita on fifteen cucumber cultivars with varying levels of resistance and susceptibility. Significant differences were found among all the cucumber cultivars regarding formation of galls, egg masses, fecundity and reproductive factor. Maximum galls and eg...
Tayfun Karataş
...ting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss). Eight-days of fasting caused a significant decrease in glucose, total protein, triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein levels as well as protein and lipid reserves in liver and muscle tissue of fish (p<0.05). The fasting period had no significant effect on the hepatic thiobarbituric acid reactive subst...
Matiyar Rahaman Khan1,*, Sabayasachi Pal2, Amit Singh2, Ashok Dhananjaybhai Patel3, Bhagubhai Ambaram Patel3, Tushar Manohar Ghule2 and Victor Phani1
...t knot nematode (Meloidogyne indica) on the roots of citrus and Bt-cotton was found in Gujarat of India. The detection of M. indica and its diagnostic symptoms on some hosts (citrus and Bt-cotton) was recorded and the nematode species was authentically identified as Meloidogyne indica Whitehead, 1968 which was first time described on citrus from Delhi, India. The species is further characterized based on...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...ly infested with Meloidogyne incognita in Layyah District. Six okra genotypes, including 19,224; 19,235; Pusa Swami; Ikra-1; Ikra-2; and Sabzperi China Red, were selected from our previous study according to their galling index and level of susceptibility. These genotypes were used as test genotypes for the interaction between M. incognita and the entomopathogenic fungus, Lecanicillium muscarium. The initial inoculum was measured be...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
...sis (CVL). A total of 30 dogs, comprising 10 clinically healthy and 20 dogs with leishmaniasis were enrolled in this study. The diagnosis of canine visceral leishmaniasis was performed by the immune fluorescence antibody test with antibody titter ≥1:128 and lymph node smear examination. In blood samples of dogs with visceral leishmaniasis, levels of malondialdehyde, total antioxidant ca...
Aisha Siddiqua1*, Munir Ahmad1 and Nusrat Habib2
...alysis and Multinomial Endogenous Switching (MES) regression model to analyze and compare the impact of different adaptation strategies in isolation or in combination on yield. The primary data of ‘Climate Change Impact Survey 2012-13’ used for this purpose. Crop-specific strategies of change in the time of sowing, use of inorganic fertilizer, change in irrigation, and varietal change. Change in irrigation practice gave rise to the highest yield su...
Khalid Khan1, Saima Liaqat2*, Somaiya Rasheed1 and Ihsanullah Kakar3
...s. However, orthodox and dogmatic methods, inadequate awareness and lack of animals’ hospitals lead to hamper livestock efficiency than the potential. Therefore, it is urged that government should make veterinary services available to the farmers at their door steps to improve their income, livelihood and standard of life. Moreover, this can be one of a feasible solution to the problems of poverty, malnourishment and starvation in backward and undevelope...
Muhammad Arshad Hussain1 and Tariq Mukhtar2,*
...-knot nematodes (Meloidogyne spp.). As very little information is available on the distribution of root-knot nematodes in okra plantations, therefore, in the present studies surveys were conducted to record incidence, prevalence, and severity of root-knot nematode species associated with okra in the major vegetable growing districts of the Punjab province of Pakistan. The overall incidence of 39%, prevalence of 85% and severity of 4.1 in terms of the ga...
Shamsudin Bojang1, Idris Abd Ghani2, Jugah Kadir1, Adamu Saidu Paiko1, Yasir Iftikhar3 and Muhammad Kamran3,4,*
...ia for nematode, Meloidogyne graminis infections on the turf grasses. Samples were collected based on the sparsely growth and chlorotic appearance of the greens. A total of 36 soils and roots sample were collected. Scanning electron microscopy (SEM) was used to identify the parasitic nematode. Both the field symptoms and SEM micrographs confirmed that the nematode isolate was M. graminis. Since this nematode has been known to damage the greens an...
