Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...e damage to sperm during cryopreservation is one of the main causes of decline in fertilizing potential. Present study aimed at evaluating the role of melatonin in extender for its cryoprotective effect on post-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates we...
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...as dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO + 20% FBS was used as the standard cryopreservation solution. After 2 months, MTT assay showed significant differences in the proportion of adherent viable ce...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...ddition.
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Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...cation of L-cysteine for cryopreservation of Achai and Holstein Friesian bull semen under the subtropical conditions of Peshawar region of Pakistan. Semen of six adult bulls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental material at Government Cattle Breeding and Dairy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 42°C tempe...
Amit Sharma1* and Pankaj Sood2
...iques. ...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...rrolidone in extender on cryopreservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concen...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...idative stress OS during cryopreservation. The excessive reactive oxygen species (ROS) and the capacity of the antioxidants could affect the OS level in the frozen semen. This study was to determine the addition of different concentrations of antioxidants to evaluate oxidative stress and fertility of frozen semen in horses. Seven stallion were used for semen collection in this study. A total of twenty ejaculates was collected from three healthy stallions twice...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...-align: justify;">During cryopreservation, the spermatozoa faces osmo-chemical, mechanical and thermal stresses, which are predominant at dilution, cooling, equilibration, freezing and thawing stages. They damage functional and morphological characteristics of sperms. Beside these exogenous stresses there is an oxidative stress which damages the spermatozoa endogenously. These stresses can be controlled with the inclusion of antioxidants in semen extender at t...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
Ghulam Jelani1, Qudratullah Kalwar1*, Asmatullah Kaka2, Abdullah Channo2, Ayyaz Ahmed1, Majid Hussain Soomro1, Hidayatullah Soomro1, Deepesh Kumar Bhuptani1 and Yar Muhammad Jalbani1
... in vitro fertilization, cryopreservation and transgenesis. Among these technologies, embryo transfer has achieved a great importance having produced numerous offspring from a genetically superior female. After the first successful transfer of mammalian embryos in 1890, it was approximately 60 years before significant progress became quite noticeable in the basic technology of embryo transfer in cattle. In embryo transfer process, embryo is collected from supe...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
...ipulation, sperm sexing, cryopreservation of ovarian tissues and germ cells and embryos, and genome resource banking for mammalian species. Such biotechnologies allow more offspring to be obtained from selected parents to increase milk and meat productivity, reduce the interval between generations, therapy of diseases and conservation of endangered mammalian species. Practically, current reproductive biotechnologies are species-specific because of differences ...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...ty of sperm medium after cryopreservation. Tris-egg yolk extender with different levels (0, 4, 8, 12, and 14 mg/ml) of propolis ethanolic extract (PEE) were used. Semen was collected once/week for 20 weeks from five buffalo bulls aged 3.5–5 years. The collected semen was pooled and distributed into five equal fractions, each fraction was diluted with Tris-extender at each level of PEE (PEE0, PEE1, PEE2, PEE3, and PEE4). Semen was gradually diluted at 37â...
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
...t critical factor in the cryopreservation of milt. In the present study, variability between spermatological parameters of induced and non-induced C. carpio was documented for a period of 7 months. Further, the non-induced milt was stored for 24 h at three dilution ratio (1:10, 1:20 and 1:40) and spermatological parameters were analysed. Then the milt was cryopreserved with the best dilution ratio using 3 freezing protocols with two step cooling profile in pro...
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
...e and post thawing cryopreservation in gaga chicken semen. The research material was 10 male Gaga chickens who were 10 months old, healthy, and normal reproductive organs. Semen was collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopically and microscopically. The sperm used had a...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...nt during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice weekly at the Semangga District Animal Health Center from August to September 2023. Semen diluted to a minimum of 25 million/mL is packaged in 0.25 mL straws. Treat Andromed® as a comparison, P0 tris egg yolk (TYE), P1 TYE+MYE 1 mL, P2 TYE+MYE 2 mL. The parameters observed include fresh semen quality, spe...
