Giselle R.R. Ayres* and Brandão P.E
...CoV) belongs to the Gammacoronavirus genus from the Coronaviridae family and Nidovirales order. Coronaviruses have a single-stranded positive RNA genome, corresponding to the largest known RNA genome, with about 27kb for AvCoV. The first 5’ two thirds of this genome, correspondent to a wide ORF1 (also called replicase gene), code for non-structural proteins (nsps) that are enrolled i...
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...ercutaneous transluminal coronary angioplasty (PTCA) as well as translational approaches. In this Experiment, we established a unique murine model of femoral artery injury through a home-made wire. The mouse model of vascular injury may be used to explore the molecular mechanism of post-angioplasty restenosis with lowest costing....
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
... triggered by pathogenic coronavirus causing watery diarrhea and high mortality in piglets. This post-viral syndrome results in severe economic losses in the pig industry. However, limited information on the porcine epidemic diarrhea virus-infected pigs is available. Thus, the objective of this study was to identify transcriptomes with RNA sequencing (RNA-seq) in peripheral blood mononucleated cell (PBMC) of piglets, Large White x Landrace crossbreds, infected...
Carolina Torres Alejo1,2, Laila Andreia Rodrigues Beserra1,2, Paulo Eduardo Brandão1,2, Luis Ramiro Luna Espinoza1,2 and Fabio Gregori1,2*
...VA, RVD, and RVF), avian coronavirus (ACoV), and avian astrovirus (AAstV), using PCR reactions followed by nucleotide sequencing and analysis. Twenty-nine pools (29/59; 49%) appeared positive for AAstV, 6 pools (6/59; 10%) were positive for RVD, forty-three pools (43/59; 72.8%) were positive for ACoV, and twenty-four pools (24/59; 40.67%) presented concomitant viral infections of two or three investigated agents. The relative high frequency of occurrence of th...
Mohsan Ullah Goraya1,2, Liaqat Ali1,2 and Iqra Younis3
...cluding influenza virus, coronaviruses and herpes viruses are important pathogens that cause significant losses in the poultry industry. However, successful establishment of infection in the respiratory tract switch on the pathogen recognition by innate immune receptors and establish first line of defense against viral infections. When specific cellular receptors recognize a virus they trigger the translation of inflammatory cytokine and chemokines leading to ...
Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2*
...caused chiefly by canine coronavirus (CCoV) and canine parvovirus (CPV). While, CPV is considered the most important cause of diarrhea, studies have shown an increasing prevalence and importance of CCoV around the world. Fatal CCoV infections have been described, however, there is limited information on the molecular diversity of CCoV in many parts of the world. In this study, canine fecal samples from diverse States of Brazil were screened by PCRs for CCoV an...
Ashraf Sayed Awaad1 and Mohamed Zaki Fathy2*
...raphed and compared with coronal and sagittal sections of CT images. While transverse and parasagittal US were performed in goats over the selected new site (last inter-lumber space (L5-L6). The latter was compared with the L6-S space by gross anatomy, CT and image analysis. The results showed wider lumen of the vertebral canal at L5- L6 and the space between the inner part of the vertebrae and the spinal cord is wider than those at the level of L6- S. Moreove...
Iqra Arooj1,* and Shahida Hasnain2
...ascular diseases such as coronary heart disease (CHD) are a growing health concern in developing and under-developed countries. Some of their risk factors are smoking, obesity, diabetes, family history, high blood pressure etc. Previous genome wide association studies have identified association of large number of loci with risk of CHD in different populations. The purpose of this study was to determine the association of 6q25.1/rs6922269 and 9p21.3/rs1333049 ...
Manzoor Hussain1, Muhammad Awais2, Farhatullah3, Saiqa Bibi1, Muhammad Rameez Khan3, Afifa Khalid1 and Sajid Ali3*
Imad Hassan1, Bilal-ur-Rehman2, Muhammad Amir2, Faheem Ali2*, Salman Ilahi2 and Amjad Ali3
...ts stability due to back corona effect. The filtration system proposed in this paper shows the ability of an efficient tool for controlling the flow of particulate pollutant matter from biomass combustion under particular conditions.
...
Zeeshan Ahmad Bhutta1,2*, Muhammad Fakhar-e-Alam Kulyar3*, Muhammad Shoaib4, Wangyuan Yao3, Muhammad Asif5, Shah Nawaz6, Moazam Ali7 and Ayesha Kanwal8
Exploratory Study on the Psychological Impact of COVD-19 on Students
...e strike of severe novel coronavirus outbreak (COVID-19) on domestic students who were at high risk of exposure. A cross sectional study was conducted through a semi structured questionnaire. Recruitment of participants was done from different universities of Pakistan. The results showed that the students are more concerned about their family members (p=0.00). Under this influence they have increased the frequency of handwashing and use masks when they have to...
Md. Sahidur Rahman
Origin and Spillover of Coronaviruses: Prospects of One Health Action
...t as a reservoir of alphacoronavirus and betacoronavirus whereas, gamacoronavirus and deltacoronavirus originate in avian species. Domestic animals also play a critical role in disease transmission as they are found seropositive against different coronaviruses. At least seven coronav...
Mardan Aghabey Turghan1, Roller MaMing1*, Li Weidong2, Di Jie1 and Xu Guohua1
...Warbler (Phylloscopus coronatus), Blue-cheeked Bee-eater (Merops persicus), Robin Accentor (Prunella rubeculoides), Tibetan Rosefinch (Carpodacus roborowskii) and Japanese Sparrowhawk (Accipiter gularis) are being reported for the first time from Xinjiang. Among them, Blue-cheeked Bee-eater is reported from China for the first time.
...
Muhammad Akram Muneer1, Khalid Munir1, Ghulam Abbas1’*, Isra Munir1, Mumtaz Ahmad Khan1, Asif Iqbal1, Munsoor ud Din Ahmad1, Muhammad Arshad Javid2, Zareen Fatima1 and Maria Arshad1
...e EastRespiratorySyndromecoronavirus(MERS-CoV)emerged as very importantissuesduringlastthree decadesas these infectionscaused quitelargenumberofhumandeathsworldwide.Coronavirusesaresingle-strandedpositive sense RNA viruses which mainly in past were considered responsible for high percentage of (around 30%) of common cold/flu cases. Viruses causing SARS, MERS and COVID-19 are members of family Coro...
Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3
... characterization of betacoronavirus 1 (bovine coronavirus) BCoV in newborn gastroenteritis calves in the Delta Region, Menofia Province, Egypt, also for isolation and identification of the local circulating BCoV strain for further diagnosis or vaccination. In cattle, Betacoronavirus 1 bovine coronavirus (BCoV) is primarily involved in enteric infections...
Arsalan Rasheed1, Tahir Usman2,*, Sadaf Niaz1, Irfan Khattak2, Saira Gul2, Nawab Ali1,Naimat Ullah Khan2, Hazrat Ali2, and Mian Saeed Sarwar2
...r and spring. Genomes of coronaviruses have large enveloped +RNA. First human corona viruses, causing respiratory diseases, were identified in 1960s, but they were not considered highly pathogenic to humans until severe respiratory syndrome broke out in China during 2003. Middle East Respiratory Syndrome, Severe Acute Respiratory Syndrome, and novel Corona Virus Disease (nCOVID-19) or Seve...
Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3
...ed during replication of coronaviruses including IBV which called quasispecies. Serotypic and or genotypic classification of IBV is mainly based on the S1 subunit of Spike (S) gene. In the present study, thirty tracheal samples of broiler flocks suspected to be infected with IBV were collected from different Egyptian governorates during 2012. Isolation and genetic characterizations were carried out for only ten samples which were positive for IBVs. Multiple nu...
Coronavirus Disease (COVID-19) with Special Reference to Pakistan: A Review on its Different Aspects
Sahar Mubashar*, Tariq Mukhtar and Nasir Ahmad Khan
...-size: small;">The novel coronavirus took the whole world by storm and in no time spread across the globe and claimed many lives. The most affected countries of the world are USA, Brazil, India, Italy, Iran, Spain, Russia, South Africa, United Kingdom and Peru. In Pakistan, the pilgrims from Iran became the major source of spread of the virus in the country, as the Government did not impose quarantine measures to restrict their movement. In Pakistan, the most ...
Roheela Yasmeen1*, Nida Mobeen1 and Samia Chaudhry2
...age, arrhythmias, severe coronary syndrome and venous thromboembolic complications. Patients of COVID-19 having already cardiovascular problems showed severe symptoms of this disease and have greater chance of death. This review article especially pays attention on cardiovascular symptoms of COVID-19 and its enhanced effect on patients with already existing cardiovascular diseases.
...
Othman N. O. Mansour 1, Naglaa Hagag 2, Momtaz A. Shahein 1, Sayed S. Hassan 1 Ahmed
A. EL-Sanousi 3 and Mohamed A. Shalaby3
...l disease of
coronaviridae family which appeared firstly in Kingdom of Saudi Arabia. MERS affects the lower
respiratory tract of human. There are many researches which emphasized that camels are carrier for
MERS-CoV.
Objective: This study aim to know the extent of prevalence of new Exotic diseases like MERS among
imported camels from some endemic countries in Africa, and to check our local breeds of c...
Mian Ibad Ali Shah*, Yasir Saleem Afridi and Laiq Hasan
... to understand the novel coronavirus using a global call. In response to but not limited to the call and extending the work to similar global pandemics, this work has been conducted on a dataset acquired from Pakistan’s COVID data. Analysis of the statistics and trends has been carried out and recommendations regarding the precautionary measures and implementation of controlling techniques for new cases using controllable factors have been provided. The ...
Muhammad Hamza Tariq1, Mahjabeen Akram2 and Yasir Sharif3*
...s caused by a novel beta coronavirus strain known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Once SARS-CoV-2 manages to enter the body, it identifies and binds to the angiotensin converting enzyme 2 (ACE2) receptors through the binding receptor of Spike Protein (S-protein). The present study aimed to investigate the phytochemicals as potential inhibitors of the binding domain of S protein so that the bi...
Zheng Tan*, Li Li, Yuxin Song, Meirong Tian and Fan Jia
... patients diagnosed with coronary heart disease (CHD) were divided them into three groups, each containing 50 patients, namely stable angina pectoris (SAP) group, unstable angina pectoris (UAP) group, and acute myocardial infarction (AMI) group. Fifth healthy people were enrolled as control. The changes in serum endothelin, brain natriuretic peptide and high sensitive c-reactive protein in patients with CHD were observed and analyzed. The levels of serum endot...
Zunping Ke1, Chao Li2, Qiang Liu3, Gang Bai4, Junfeng Wang5, Ming Zhou5 and Xiao Dong5,*
... tetralogy of Fallot and coronary artery to bronchial arterial fistula is reported. The communication of fistula arising from the proximal left circumflex coronary artery was discovered by routine coronary artery angiography before correction surgery. The patient then underwent TOF correction surgery. Considering the significance of the fistula, it better be found out and ligated prior to ...
Roheela Yasmeen1* and Samia Chaudhry2
...align: justify;">A novel coronavirus commonly known as COVID-19 has resulted in an ongoing outbreak of viral pneumonia. This pandemic started from Wuhan City, China and has spread throughout most parts of the world (210 countries). COVID-19 is a large sized, enveloped, positive stranded RNA virus. Out of the four known genera, alpha and beta corona viruses are the most commonly recognized viruses infecting human beings. COVI...
Muhammad Altaf1* Arshad Mahmood Abbasi2, Muhammad Shoaib Amjad3, Sadia Naseer1 and Muhammad Umair4
...fied into 4 genera: Alphacoronavirus, Betacoronavirus, Gammacoronavirus and Deltacoronavirus. Betacoronavirus most probably originated from bats and the virus may have jumped to avian species and evolved as Deltacoronavirus group. The avian coronav...
Tehseen Ali Jilani1*, Muhammad Saleem Jilani2, Javeria Sherani3, Kashif Waseem2, Muhammad Sohail Khan2, Hasnain Saleem3, Abdul Manan2, Rashid Jawad2 and Sami Ullah4
Muneeza Zafar1,2,3, Fazli Rabbi Awan2,*, Munazza Raza Mirza3,*, Sumaira Nishat2,4, Sajid Ali Rajput5 and Imran Riaz Malik1,*
... may result in premature coronary artery disease, familial hypobetalipoproteinemia, hypocholesterolemia, mono-genic dyslipisimias and other atherogenic events in CVD. Here we evaluated the impact of all missense and non-coding single nucleotide polymorphisms (SNPs) of APOB retrieved from dbSNP using 17 different computational tools and further evaluated the structural impact of these convergent deleterious SNPs on APOB through HOPE. We found 9 missenses, 15 in...
Ayma Aftab, Samia Afzal* and Muhammad Idrees
...een used in detection of corona virus. This case study emphasizes the importance of HRCT for early and confirm diagnosis of COVID-19 whereas RT-PCR results can vary. This process may show negative results and is time consuming....
Abdul Kabir1, Muhammad Rasheed2, Hubdar Ali Kaleri5, Depeesh Kumar Bhuptani3, Mithan Kumar4, Raza Ali Mangi6, Abdul Wahid Solangi4, Sheva Dari1, Saqib Kakar4, Panah Munir4, Ekra Akbar4 and Rameez Raja Kaleri4,5*
...n: justify;">Pandemic of corona virus was started on the 11 March of 2020 year in Wuhan city of Peoples Republic of China by SARSCov 2 novel corona virus. In Covid 19 lockdown situations different types of industries got suffered and including livestock and poultry farming at high range. In the December of 2019, outbreak of corona virus started in China. The most common symptoms of this vi...
Farah Naz1*, Asad Umair2 and Ghazala Noureen3
...lth. The outbreak of the corona virus plunged the world in a state of chaos and uncertainty. As fear and anxiety built up, governments around the world responded to the pandemic by imposing long, nationwide lockdowns in an attempt to “flatten the curve”-a term that refers slowing down the spread of the disease in order to prevent healthcare facilities from being overwhelmed by too many new patients. However, lockdowns and related strategies designe...
Saba Rehman1, Faisal Salih Hayat1, Sadia Norin2, Abdul Aziz1, Siddiq Ur Rahman1* and Noor ul Haq1*
...ute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly evolved in the last couple of years. This has created major havoc and concern because it has affected millions of people around the world. The new variants of covid-19 are classified into two types, VOI (variant of interest) and VOC (variant of concern). The major variants of concern (VOCs) have shared mutations in severe acute respiratory syndrome coronavirus 2...
Barbosa Carla1*, Gregori Fabio2, Thomazelli Luciano1, Oliveira Amanda1, Araújo Jansen1, Ometto Tatiana1, Marcatti Roberta3, Nardi Marcelo3, Paludo Danielle4, Utecht Nathalia1 and Edison Durigon1
...spersed by birds are the coronaviruses (CoVs). Despite its relevance and complexity, studies on the detection and genetic characterization of these viruses are scarce, especially in South American wild birds. Thus, this paper aims to detect and discuss the diversity of these agents in birds at migratory stopping and wintering sites, along Brazilian Segment of the Atlantic Flyway. Therefore, 738 avian samples from different regions of Brazil were subjected to a...
Caiping Duan
...t puncture point). After coronary angiography in each group, the serum nitric oxide (NO), endothelin-1 (ET-1) and cardiac troponin (cTnT) contents in different treatment groups were detected by enzyme-linked immunosorbent assay. Coronary blood samples were obtained during coronary angiography. Expressions of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-&...
Jihan Abd Wahab, Nur Bahiah Mohamed Haris* and Jasmin Arif Shah
Somia Eissa Sharawi
...y by driving the ongoing coronavirus disease (COVID-19) pandemic. COVID-19 in humans is characterized by a wide range of symptoms, from asymptomatic to mild or severe illnesses, including death. The existing literature shows the roles of insect vectors in aiding the transmission of viral pathogens and the possible roles of some insects in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. In this stud...
Ahmad Shahzaib1*, Tabish Raza2 and Aisha Areej2
...ute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel respiratory infection that has caused the most recent epidemic of coronavirus disease 2019 (COVID-19). Although COVID-19 has a wide range of clinical manifestations, affecting many vital organs, the virus enters via the respiratory system, and the lungs are the leading site of the infection. The virus spreads primarily by respiratory droplets generated by coughin...
Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li
...avirus (BRV), and bovine coronavirus (BCV) have increased calves morbidity and mortality in Hebei Province. To detect these three pathogens simultaneously, we designed specific primers based on the conserved gene sequences of the three pathogens available in GenBank. After optimization of the reaction conditions and system, we successfully established a novel multiplex PCR method for detection of the aforementioned three pathogens. The results show that the am...
Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.
...expression of the bovine coronavirus (BCV) nucleocapsid protein was carried out in a baculovirus expression system. The specific RT-PCR product of N gene was cut and extracted from gel using DNA gel extraction kit (Millipore). Eluted DNA was successfully cloned in pBlueBac4.5N5-His TOPO TA baculovirus transfer vector and transformed in chemically competent E. coli. A modified colony PCR assay was utilized to identify the positive bacterial colonies that harbor...
Makmun1, Imam Mujahidin Fahmid2, Muhammad Saleh S. Ali2*, Muhammad Yamin Saud2, Rahmadanih3
Qing Cao1, Wei Jiao2, Huihui Lu1, Jing Zhang2, Meiling Ren3, Yan Xu1* and Shuyang Hu1*
...II diabetes mellitus and coronary heart disease (CHD) on the hypercoagulability and thromboembolism. A total of 236 patients with type II diabetes mellitus and CHD who were treated in 904 Hospital of Joint Logistic Support Force of PLA were enrolled between January 2018 and January 2020. These patients, according to their 2 h postprandial glucose (2hPG), were divided into the hyperglycemia group (n = 126) and hypoglycemia group (n = 112). Patients in the hypog...
Qing Cao1, Wei Jiao2, Huihui Lu1, Jing Zhang2, Meiling Ren3, Yan Xu1* and Shuyang Hu1*
...II diabetes mellitus and coronary heart disease (CHD) on the hypercoagulability and thromboembolism. A total of 236 patients with type II diabetes mellitus and CHD who were treated in 904 Hospital of Joint Logistic Support Force of PLA were enrolled between January 2018 and January 2020. These patients, according to their 2 h postprandial glucose (2hPG), were divided into the hyperglycemia group (n = 126) and hypoglycemia group (n = 112). Patients in the hypog...
Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*
...mice induced with canine coronavirus (CCoV) vaccine. The research utilized male mice (Mus musculus) aged two to three months, divided into three groups: a control group administered with normal saline (D0), a CCoV-induced group (D1), and a group induced with CCoV along with 1 mL/kg body weight of Lactobacillus acidophilus for seven days (D2). The CCoV vaccine was administered subcutaneously at 0.5 mL/kg body weight, while the Lactobacillus acidophilus isolate ...
