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Muhammad Mushtaq

E-mail | mushtaq@uaar.edu.pk

... Further trials with low concentrations of strychnine alkaloid and decreasing the poison-baiting duration are suggested.

 

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Muhammad Rizwan Tariq1*, Muhammad Issa Khan1, Aysha Sameen1, Mahr un Nisa2

 

...urated fatty acid (PUFA) concentration that contributes 8.5% of the total fatty acids. Rabbits were reared for the period of eight weeks. Enrichment of 7% flaxseed in the diet of rabbits significantly enhanced growth performance of rabbits and improved poly unsaturated fatty acids in the meat of rabbits as compared to other groups.

 

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Ghulam Ali Bajwa and Muhammad Arshad Khan

... fertilizer application, concentration of N, P, K and Fe in the soil almost doubled. Zinc and Cu contents decreased significantly (p<0.01). The highest N, P and K were found in mulberry variety PFI-1, M. latifolia and Karyansuban, respectively. The highest Cu and Mn were found in Kanmasi. The highest Zn and Fe were found in Husang and Karyansuban, respectively. A significant variation in uptake and retention of macro- and micro nutrients was found in the mu...

Gultaj Hussain1, Abdur Rahman1*, Tariq Hussain2, Siraj Uddin1, Tariq Ali3

E-mail | abdur_rahman@aup.edu.pk

...f citric and lactic acid concentrations effect were maximum for 5.0%, followed by 3.0% and least for 1.0% on E. coli and Salmonella load on beef surfaces. In addition, citric acid was more effective than lactic acid at all concentrations (1.0, 3.0 and 5.0%) in reducing E. coli and Salmonella growth. The citric acid at 5.0% was found significantly effective treatment in reducing E. coli O157: H7 and S. typhymurium population ...

Mudasir Irfan Dar*, Fareed Ahmad Khan and Farha Rehman

E-mail | irfanmudasir@gmail.com

...ere exposed to different concentrations of the insecticide, ranging from 0 to 40 grams active ingredient per hectare (g.a.i ha-1) through foliar spray. Analyses were done at days 3, 7, and 15 after treatment. Lipid peroxidation rates and contents of proline, ascorbate (ASC), glutathione (GLU), antioxidative enzymes; superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were assessed. Imidacloprid enhanced malondi...

Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3

... ml-1. Median effective concentration (EC50) ranged between 9.87x105 and 21.30x105 conidia ml-1 for 3rd to 5th instars. Median effective time (ET50) ranged between 3.3 days and 8.2 days for different tested concentrations. A significant (p<0.05) 2nd degree correlation between temperature and B. bassiana infectivity was analysed. Beauveria bassiana infection was in range of 27-30ºC. Based on these findings, it is con...

John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1

...ss-react with comparable concentrations of E. coli or L929 murine host cells, the assay did cross-react strongly with members of the spotted fever and scrub typhus groups, making the assay specific only to the Rickettsiaceae family level (Rickettsia and Orientia). When conducted in < 100% human serum or a tick homogenate, the fluorescent aptamer assay maintained sensitive detection of Rickettsia typhi

Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3

...ositive) were having the concentration of aflatoxin B1 higher than the permissible level which is 5 ppb for concentrates as recommended by European Communities. It is concluded that the concentrate available for goat feeding is having aflatoxin B1 and its concentration is higher than the permissible level.  

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Nazish Huma Khan*, Mohammad Nafees and Adila Bashir

... showed that the average concentrations of metals in soils of orchard and non-orchard fields were as chromium (Cr) 56.9 and 52.2 mg kg-1, Ni 29.8 and 27.9 mg kg-1 and Cd 2.46 and 1.11 mg kg-1, respectively. The risk contribution from Cd, Cr and Ni were significant in crop seeds of orchard field (THQ>1). The substantial load of heavy metals in studied fields showed the anthropogenic source of pollution. Therefore regular moni...

 Ishfaq Ahmed, Ihsan Mabood Qazi and Suraiya Jamal

...flour (BRF) at different concentrations (0, 20, 40, 60, 80 and 100 g/100 g) on the proximate compositions and functional properties. It was found that percent protein (8.93–12.25%), ash (0.52–1.01%) and moisture contents (4.47–7.09%) showed significant (P < 0.05) increase, while fat content (1.41-1.17%) showed significant (P < 0.05) decrease by blending WF with BRF. Similarly, water solubility index (WSI), water absorption index (WAI) a...

 Farmanullah Khan, Samad Khan, Wiqar Ahmad and Imran Khan

...ant on Cu, Zn, Fe and Mn concentration. Economic analysis showed that NPK75FYM20 rewarded the maximum net income of Rs. 43108 ha-1, indicating its superiority for profitable wheat yield under rain-fed conditions. It can be concluded from these results that the application of plant nutrients from a variety of natural and manufactured fertilizer sources improve crop yield as well as augment the fertility of soils suffering from water erosion hazards.

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 Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid

 
...ect on human health. The concentration of heavy metals (Pb, Cd, Cr, Fe, Mn and Zn) present in poultry egg and meat were determined in the following three districts; Peshawar, Dir Lower and Malakand of Khyber Pakhtunkhwa by using atomic absorption spectrophotometer. In all the three districts the egg albumen was found to contain significantly higher levels of Pb, Cd and Cr as compared to egg yolk which contains significantly higher levels of essential elements ...

Mian Shamas Murtaza1, Aysha Sameen1, Nuzhat Huma1 and Fatma Hussain2

...dually on increasing the concentration of gums and the samples having guar gum were less hard. On sensory evaluation, the maximum scores were awarded to full fat cheese, while the negative control attained the minimum liking. Addition of gums improved the sensory acceptability of cheese. The sample containing 0.45% guar gum was awarded the scores comparable to the full fat cheese for most of the traits. Hence, it was concluded that hydrocolloids particularly g...

Abida Saleem, Sajida Parveen and Muhammad Jamal Khan

...ssess the equilibrium Zn concentration (EZnC). The difference between the IZnA and EZnC was assumed to be adsorbed. Results showed that FYM, PM or BC yielded lower EZnC and higher Zn adsorption (x/m) at higher IZnA but at lower IZnA< 20 mg Zn L-1 the reverse was observed. The data in non-treated soils were fit to both Langmuir and Freundlich but in treated soils they were fit only when adsorption values for initial IZnA were disregarded. The adsorption, as ...

Sadiq Hussain, Muhammad Sharif, Sarmad Khan, Fazli Wahid, Hina Nihar, Wiqar Ahmad, Imran Khan, Nadeem Haider and Tabassum Yaseen

... P on the basis of its P concentration. The recommended dose of N @ 120 kg ha-1 was applied in the form of vermicompost and urea, while K was applied in the form of sulphate of potash. Spores of mycorrhiza were isolated from fresh growing crop of berseem at the University research farm and used as inoculums in this experiment. Results showed the maximum grain yield of 15.09 and 14.7 g pot-1, highest total dry matter yield of 36.3 g and 36 g pot-1, maximum root...

Muhammad Zahid1, Muhammad Hamid Bashir1*, Bilal Saeed Khan1 and Muhammad Shahid2

... N. barkeri at different concentrations under controlled laboratory conditions. The results showed 25%, 30%, 70%, 50% and 65% mortality occurred by tested pesticides at field relevant doses after 144 h respectively. Missing mites data indicated repellency due to pesticides, was highest at the start of experiment, and then these acclimatized on leaf discs. Thiacloprid having lowest LC50 value 295 and proved toxic for the predator while diafenthiuron and pyripro...

Ayanda Opeyemi Isaac,1,* Oniye Sonnie Joshua2 and Auta Jehu2

 

...exposed to varying acute concentrations of glyphosate and paraquat. The fishes responded, exhibiting different behavioural abnormalities like hyperactivity, abnormal swimming, restlessness, loss of equilibrum and haemorrhage. Observation of opercular ventilation count (OVC), tail fin movemant rate (TMR) and air gulping index (AGI) showed a marked difference between control and exposed fishes, indicating that the herbicides negatively impact on these parameters...

Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*

...typic traits such as low concentrations of FSH, LH and T and delayed puberty. In conclusion, mutations in GNRHR may cause delay in male puberty in Pakistani population.

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Afzaal Ahmad Naseem1*, Maleeha Akram1, Sarwat Jahan2, Kiran Afshan2, Zubaria Iqbal1, Faheem Tahir3, Mazhar Qayyum1 and Syed Shakeel Raza Rizvi1

...=27 boys/age group). The concentrations of GH and T were measured using specific ELISA systems and LGV was calculated for each age/stage group. Pearson correlation r and ANOVA were employed. GH and LGV exhibited age related positive correlations from 1 to 20 years and developmental stage dependent positive correlations during infancy, pre-puberty, early, mid and late puberty/adolescence. T and LGV were positively correlated from 1st to 16th year and negatively...

Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2

...and the maximum nontoxic concentration of the extract was determined using DPPH assay and MTT method, respectively. Serial concentrations of the extract not exceed the maximum nontoxic concentration were used against Vero cells for anti-HSV-1 and ant-HSV-2 effects by plaque assay. The results of the phytochemical analysis showed that the extract has high polyphenolic content while has rela...

Shakeel Ahmed* and Sarwat Jahan

...s. The plasma adipokines concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly increased plasma adiponectin (P<0.001), while decreased leptin (P<0.001) and resistin (P<0.01) concentrations compared to normal fed monkeys. No significant (P>0.05) change in adiponectin levels was observed after NMS injection in both normal and metabolically stressed conditions. NM...

Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4

...ts pH (6.7 to 7.4), high concentration of phosphorus (4.50% and 4.33%) and nitrogen (3.26% and 2.37%) favor seed germination, enhance root growth and soil fertility.

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Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2

...ine oocytes at different concentrations. The results showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos in the ...

Faiqah Ramzan*1 and Muhammad Haris Ramzan2

...ally in different dosage concentrations (1μg, 1 ng, and 10 ρg) to adult male mice twice daily for 12 days. Prostatic citric acid levels were determined photometrically. The levels decreased significantly at all tested doses. Intermittent administration of Kisspeptin –10 negatively regulates the prostatic citric acid levels in adult male mice.

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Muhammad Shafiq and Tahir Maqsood

...arding chemical analysis concentration of boron in both wheat straw and grains increased with B application but there was no effect of fertilizer B on NPK concentration of straw and grains. These results are very heartening; signifying that for any soil, B adsorption isotherm need to be worked out for location precise fertilizer B use.

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Dilawar Hussain* and Abdul Mateen

...at both 2 and 4 ppm AFB1 concentrations have almost the same effect on the growth parameters tested except in the case of PER. T3 (0.5% 4TX+2 ppm AFB1) showed better PER (31.42±1.74) when compared to T4 (0.5% 4TX+ 4 ppm AFB1) group (27.76±0.67). Interaction between different growth parameters of tilapia fed AFB1 and 4TX supplemented diets showed that net weight gain (NWG) was significantly (p<0.01) correlated with average daily gain (ADG), spe...

G.E. Odo1*, E.J. Agwu1, N.I. Ossai2, C.O. Ezea3, J. Madu1 and V. Eneje2

... and MCHC to all exposed concentration of aluminium phosphide, significant increase in neutrophil, basophil, eosinophil and monocytes throughout the duration of exposure and decrease in the lymphocytes . The results on gill and liver tissues sampled showed concentration and time dependent significant increase (p < 0.005) in the values of malondialdehyde, catalase, superoxide dismutase, reduced glutathione, alkaline phosph...

Nighat Sultana1,2,*, Shazia Iftikhar1, Nafeesa Qudsia Hanif2 and Iffat Tahira2

... fodder, with an average concentration of 9.49 and 8.36ng/g respectively. Aflatoxin B2 was detected in only two samples (16%) (1.2 and 1.3ng/g), aflatoxin G1 and G2 were not found. Ochratoxin A was found more frequently in fresh (54.16%) than in ensiled (20.86 %) samples with a mean of 8.06 and 4ng/g respectively. Average values for total aflatoxins (TAFs) and OTA were below the permissible limits as per regulation of European commission (EU), i.e. 20ng/g and...

Wang Tian1, Huayong Zhang1,*, Jian Zhang2, Lei Zhao1, Mingsheng Miao3 and Hai Huang1

...ich provides the maximum concentrations of nutrients. Zooplankton communities were more correlated to phytoplankton community biomass than environmental factors. Results of canonical correspondence analysis (CCA) revealed that water temperature, Secchi disk depth (SD), total phosphorus and phytoplankton biomass were the most significant factors that influenced zooplankton. Strong correlations between SD and Brachionus leydigi, Chrysophyta and Diaphanosoma leuc...

Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

...indicates that Pb and Cr concentration differ significantly between AA, AG and GG genotypes of SOD1 (rs2070424) gene polymorphism in RA patients as compared to control group (p<0.05). The present finding indicates that SOD1 (rs2070424) gene polymorphism is not associated with pathogenesis of RA; however levels of Pb and Cr may impart strong influence towards the development of RA in the presence of this polymorphism.

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Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1

...rols. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts was also determined. Syzygium aromaticum was proven to have excellent antimicrobial activity against all tested microorganisms, while C. sinensis and M. piperita showed weak antimicrobial activity. Methanolic plant extracts exhibited greater antimicrobial activity than aqueous extracts. Maximum zone of inhibition exhib...

Sidra-Tul-Muntaha1, Muhammad Sagheer1*, Mansoor-ul-Hasan1 and Shahbaz Talib Sahi2

... Punjab, Pakistan. Three concentrations of each plant extract (5, 10, 15 and 20%) and synthetic pyrethroids (0.01, 0.02 and 0.03%) were evaluated in this study. We observed significant results with each treatment. For both repellent and growth inhibitory effects, A. indica and deltamethrin were most efficient among plant extracts and pyrethroids respectively. At highest dose rates, more than 90% repellency was recorded with both A. indica and deltamethrin. Upt...

Salma Shaheen, Mumtaz Khan, Muhammad Jamil Khan, Saleem Jilani, Zarina Bibi, Muhammad Munir and Mehwish Kiran

 

...M. Maximum Fe, Cu and Mn concentrations were found where pressmud + EM was applied. Dietary fiber, vitamin C and crude proteins were also increased in spinach with pressmud + EM application. It was concluded that EM-inoculated pressmud has higher potential to increases soil fertility as well as stimulate spinach growth and quality. Therefore, warrants further testing under field conditions.

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Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

...ethod. However, the same concentration of crude (1 µg.mL-1) showed 62.20 ± 2.51% growth inhibitions against Fusarium oxysporum using agar well diffusion method. Further investigation, including various chromatographic and spectroscopic analyses on the separation and isolation of potent metabolites is suggested to get a useful lead structure for development of novel antibiotics to combat resistant pathogens.

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Önder Duysak*andKübra Azdural
...s of Iskenderun Bay. The concentration of heavy metal in P. caerulea tissues tended to vary significantly between season and stations.The distribution of heavy metals in P. caerulea followed in their muscular tissues in order; Çevlik > Kaleköy> Payas > Arsuz > Dörtyol > Konacık > Yumurtalık > İskenderun for spring and Kaleköy > Payas > İskenderun > Arsuz > Dörtyol >...
Serkan Sayiner1* and Hilal Karagul2
... evaluated whether these concentrations were adequate. Heifer blood, feed samples including concentrate feed or roughage were analysed. It was found that there are significant seasonal differences for each region and regional differences in each season were due to different feeding conditions. Heifer blood selenium and plasma vitamin E levels were generally found within adequate ranges in all regions for both seasons. We suggest that feed supplements containin...
Sumra Ashraf1, Zain ul Abdin1,*, Saqi Kosar Abbas2,Rao Sohail Ahmad Khan3, Muhammad Tahir1, Sehrish Rasool1, Maryam Anwar1 and Fiaz Hussain1
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...n corpuscular hemoglobin concentration (MCHC) and platelets while total leucocytes count (TLC), neutrophils, eosinophils, basophils, lymphocytes, monocytes, red cell distribution width (RDW) and mean corpuscular volume (MCV) were increased significantly (P < 0.05) which indicated hemolytic anemia. While, serum biochemistry showed significant elevation in alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nit...
Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
...ceps femoris muscle, the concentration of N-isopropyl oxamate in blood was 0.08 mmol/L; 3) in skeletal muscles of the inhibitor group and the control group, the LDH activities were 6.30±0.50 U/mg and 10.01±0.59 U/mg, the contents of LD were 0.28±0.039 mmol/g and 0.84±0.16 mmol/g, and the ATP level were 8.15±1.03 nmol/mg and 12.06±1.23 nmol/mg (P<0.01); 5) the inhibition rates of N-isopropyl oxam...

 Abdul Wajid Khalil and Zafar Iqbal

...00g) was found in higher concentration in aerial parts than roots while some essential amino acid, Tryptophan (6.23±0.06g/100g), Arginine (3.49±0.08 g/100g), Phenylalanine (1.77±0.08 g/100g) were recorded higher in roots as compared to the aerial parts of B. procumbens. It is concluded that aerial parts may serve as a supplement for human diet as well as fodder for livestock. Detailed studies on amino acid of roots are recommended to deter...
Salman Khalid1,*, Abdul Rehman2, Mohammad Yasin Amir1, Abdul Rehman1, Imran Sarfaraz1 and Muhammad Asif1
...he values of PCV (%), Hb concentration (gm/dl) and RBCs during tick infestation.
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Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

... chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in egg...
Xiang Nong1,2,3,*, Yao-jun Yang1,2, Guang-you Yang3, Feng-zheng Chen2, Mei Tang1,2 and Gang Wang2
...rol was toxic under 0.5% concentration. Treatment under this condition also showed mite mortality of 16.67% within 12 h and 23.33% within 24 h. The insecticidal activity of stigmasterol was weaker than that of ageraphorone compound obtained from E. adenophorum Spreng. Through preliminary separation. However, in the experiments, we found an interesting phenomenon, that is, the dead state of mites treated with stigmasterol was similar to that of mi...
Adel Eid Mohamed Mahmoud*
...ues and ammonia nitrogen concentrations did not show any significant differences among groups.No significant differences were observed in meat chemical analysis of slaughtered animals among different groups. Animals fed BBP recorded insignificant increase in growth rate by 9 g daily with improvement in feeding cost by 10%.Bakery waste can replace corn grains and wheat bran in concentrate feed mixtures up to 60 % in growing lambs rations without any adverse eff...
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...of the extracts at known concentration of chlorogenic acid were then applied against different bacterial strains for antibacterial activity. The minimum inhibitory concentration of isolated compound was also measured. Two antibiotics were used as references compounds for antibacterial activities. The aqueous ethanolic extract having 968.125 µg/ml of chlorogenic acid inhibited significantly the growth of tested bacteria...

A. K.Bhattacherjee and B. K. Pandey

Dissipation of carbendazim in mango after pre- and post-harvest treatments
...at 0.05 and 0.1 per cent concentrations, respectively, following first order rate kinetics. The corresponding values in fruit pulp after 12 days were 0.57 and 1.26 mg kg . In another experiment, cold water dip treatment in carbendazim solution @0.05 and 0.1 percent was given for 10 min to a separate lot of fruit having no pre harvest spray. Dissipation of carbendazim in whole fruit again followed first order rate kinetics with 0.92 and 2.06 mg kg of the fungic...

P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi

Botanicals for control of anthracnose of bell peppers
...rom C. aromaticus at 50% concentration showed 42% radial mycelial growth inhibition in poisoned food technique, whereas 15% increased seed germination and 26% reduced disease incidence is recoded when compared to untreated control. In pot experiments, there is an increase in height and weight of the plants by 22% and 44% respectively in seeds treated with C. aromaticus when compared to chemical fungicide Bavistin and also 13% reduction in disease incidence is ...

M.S. Islam, M.Ali, and I. Ahmad

Efficacy of sedomil 72WP and recozeb 80WP to control die-back (Colletotrichum gloeosporioides) of tea in Bangladesh
... 1200, 1600 and 2000 ppm concentrations. Sedomil 72WP and recozeb 80WP caused colony growth inhibition, respectively by 75.00-90.77% and 75.88-91.33% in 2008 and 74.66-90.55% and 75.66-91.11% in 2009. In a field experiment at the main farm of Bangladesh Tea Research Institute (BTRI), both fungicides were applied as foliar spray for three times at 1.0, 1.5, 2.0 and 2.5 kg ha-1. In 2008, sedomil 72WP gave 45.52-92.78% and recozeb 80WP gave 46.47- 90.39% reductio...

A. Sajeena and T. Marimuthu

Efficacy, stability and persistence of Ganosol, a Ganoderma based fungicide against plant pathogens
...as the best emulsifiable concentration for use against the pathogen. The pre inoculation spray of the formulation was observed to produce 63.62% reduction in lesion height caused by the sheath blight pathogen. Two isoforms of poly phenol oxidase, PPO 1 and PPO 3 were observed to be induced only in Ganosol 10EC sprayed plants and were absent in control. The formulation was tested to have good emulsion stability. The antifungal activity of the formulation persis...

D.K. Hazra, Megha Pant, S.K. Raza and P. K. Patanjali

 

Formulation technology: key parameters for food safety with respect to agrochemicals use in crop protection
...hains where they undergo concentration and pro-duce potential, long term, adverse health effects. Pesticides in developing countries in Asia and Pacific region are mainly available as dust, wettable powder, emulsifiable concentrates, solutions, etc. These types of formulations are regarded now as ?conventional‘, ?old technology‘ or ?classical‘ or ?traditional‘ because of their increased in dose rate or repeated applications to get desir...

Anil Sehajpal, Saroj Arora and Parminder Kaur 

 

Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...bial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungitoxicity even at the lowest concentration, i.e. 100 ppm, with relative magnitude ...

Shrinivas Mudigoudra and Shekharappa

Evaluation of plant products aganist sorghum shoot fly, Atherigona soccata Rondani
...onosperma, Aloevera @ 5% concentration and were compared with carbofuran 3 G @ 30 kg. ha-1 to know its efficacy on shoot fly in sorghum ecosystem. The results clearly indicated that NSKE (5%) recorded less eggs of shoot fly (0.40egg/plant), per cent dead heart (14.97%) and maximum grain yield of 13.22 q.ha-1 among different plant products. The next best plant product was found to be V. negundo @ 5% in recording less incidence of shoot fly and obtaining higher ...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...rrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin. Camel raw milk produced 2.4 mg/mL of lactoferrin. The lactoferrin isolated from camel milk exhibited promising antibacterial activity against E. coli and Staphylococcus aureus. Concentration of lacto...
Sohail Ahmed, Muhammad Arshad and Babar Hassan*
...ee resins types in three concentrations were applied by brushing and dipping on wooden stakes which were exposed to termites’ for a period of 4 weeks in the field. Weight loss before and after resin treatment was indicative of effectiveness. Highest concentration of all resins significantly reduced the chances of termites’ infestation as compared to other concentrations by dipp...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...lids not fat and lactose concentrations differed (P<0.001) among the treatments but did not show (P>0.05) any linear or quadratic trends with increasing level of enzyme in the diet. Milk protein concentration linearly (P<0.001) increased with increasing level of enzyme in the diets. It is concluded that fibrolytic enzyme addition in the diets of lactating Nili Ravi buffaloes improves the dry matter intake and diet d...
Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...llel to increase in dose concentration, as compared to control and vehicle control. Morphometric studies showed significant differences in nervous, integumentary and skeletal systems against control. Exencephaly, Omphalocoel, hygroma and limb abnormalities were recorded. It is shown that Cr VI causes teratogenic effects in mice embryos.
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Obaidullah1, Zafar Ali Shah1* and Sami Ullah2
...;g/ml and 80 µg/ml concentrations versus their counterparts of Lapachol and above mentioned metallic salts.

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Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
...ed that RBC, MCV and MCH concentrations were significantly higher (P < 0.05) and the serum total protein and Mg concentrations were significantly lower (P < 0.05) in male compared to female peafowl. In conclusion, the present study provides base-line values for hematology and serum biochemistry of apparently healthy Indian adult blue peafowl of Thar and can be used as reference values to evaluate the health or diseased...
Muzammil Sattar
...ed in laboratory at four concentrations, i.e., 1, 3, 5 and 7 mg/ml in diet. Significantly higher fecundity 785.12±25.7, fertility 89.23±0.36 % of eggs and longer oviposition period 42.13±1.11 days were recorded in adults feeding on a diet containing Nu lure followed by protein hydrolysate (control diet). The F1 progeny larvae that developed from adults consuming (Nu lure 5ml) diet had the shortest larval and pupal periods...
Mehwish Faheem1,*, Saba Khaliq2 and Khalid Parvez Lone2
...i> was exposed to graded concentrations of bisphenol-A (10,100,1000μg/l) for 14 days. BPA exposure strongly elevates vtg mRNA level in fish exposed to 100μg/l but at 1000 μg/l exposure of BPA, vtg level decreased compared to 100µg/l exposed fish. These results showed that BPA has estrogenic action and cause endocrine disruption in juvenile C. catla at environmentally relevant concentration...
Ayesha Ilyas1, Hafiz Azhar Ali Khan2,* and Abdul Qadir1,*
...ta (Saunders), at 2% concentration in a free choice bioassays. Acetone, chloroform, petroleum ether and ethanol were used for extraction from leaves. Amongst the various treatments applied, the acetone extract of D. alba showed the highest repellency of 84.14%, whereas, the lowest repellency of 10.73% was observed by the chloroform extract of T. peruviana. Significant ovipositional deterrent effects of the extracts applied have been observed....
Hazrat Ali1,*, Wajid Ali1, Karim Ullah1, Fazle Akbar1, Sher Ahrar1, Irfan Ullah1, Irum Ahmad1, Ashfaq Ahmad1, Ikram Ilahi2 and Muhammad Anwar Sajad3 
...rophotometer. Highest Cu concentration of 4.52 ± 0.24 mg kg−1 wet weight was observed in muscles of M. armatus at Chakdara on River Swat while highest Zn concentration of 18.00 ± 1.88 mg kg−1 wet weight was observed in muscles of S. plagiostomus at Daggar on River Barandu. A site wise comparison of both Cu and Zn concentrations ...
Noor Islam1, Muhammad Amjad2, Ehsan ul Haq3, Elizabeth Stephen1 and Falak Naz4
... acid at three different concentrations of 25%, 50% and 100% and control were used against T. clareae in naturally infested honey bee, A. mellifera colonies in Honeybee Research Institute, National Agricultural Research Centre, Islamabad, Pakistan. The percentage of infestation by T. clareae on worker brood, number of dead/fallen mites per hive/weekon white formica sheet, percent mite mortality and honey yield per colony was determined bef...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...ckens with two different concentrations of Egy-H5 plasmid DNA 15µg and 60 µg. Results demonstrate that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vaccine. The Egy-H5 plasmid DNA...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3

 

...ved more fatal at higher concentrations than the individual application at lower concentration for pupation and adult emergence of S. litura. Apart from untreated larvae (control) maximum pupation (66.01±1.32 and 73.95±1.28 %) and adult emergence (61.87±1.71 and 67.77±1.11 %) was observed when larvae were treated with NPV (1x107 POB/ml) against 2nd and 4th i...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...ogens at three different concentration viz., R, R/2 and R/4. Combination of bio agents (T+P) gave maximum results at R concentration against R. bataticola and M. incognita.
...
Kulsoom Akhter1, Tahseen Ghous1,2,Saiqa Andleeb3,*, Faiz-ul-Hassan Nasim4, Samina Ejaz5, Zain-ul-Abdin1, Bilal Ahmed Khan1 and Muhammed Naeem Ahmed1
...7401. At maximum studied concentration of metal ions (250 mgl-1), in above sequence, the strains accumulated 118.2, 121.87 and 90 mg/g Cr (VI), 135, 127.5 and 146.25 mg/g Ni (II) and 120, 129.37, 135 mg/g Cd(II). Langmuir and Freundlich models were applied and found suitable to describe biosorption of selected metals by the isolated strains.
...

Fawad Ali1*, Hidayat Ullah2, Ikhtiar Khan1

...ertain toxic heavy metal concentration in the wastewater, irrigated soil, their accumulation in vegetables, and potential human health risks. The results reveal that Cr concentration in the wastewater, Cd and Zn in soil and in vegetables have been found well above the permissible limits of World Health Organization (WHO) which may lead to a detrimental effect on soil quality, leading to food contamination. The extractable
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...>oC, Protocol III (concentration of dye in staining solution); slides were stained with 1, 5, 200, 500, or 1000µg/mL solution. The slides were observed, intensity of staining and debris presence in samples. The debris presence was reduced (P<0.05) due to washing of semen sample. Intensity of staining increased (P<0.05) due to washing and heating during incubation in tampon solution. However, single and double washing and type of buffer did no...

Syed Tanveer Shah* and Muhammad Sajid 

...lphate) at three calcium concentrations (0.5, 0.75 and 1.0%) stored for 30 days at 10 days interval. The experimental results showed that calcium sources, concentration and storage duration significantly affected all the studied attributes. More fruit firmness (5.57 and 5.69 kg cm2), less Total soluble solids [TSS] (8.57 and 8.26 °Brix) and TSS-acid ratio (12.62 and 11.48) were recorded by the alone application of calciu...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*

 

...ere exposed to different concentrations of LET. At 130 dph, sex ratio was recorded. The results showed that continuous exposure to LET induced the changes of sex ratio. High concentration of LET can promote male-biased sex ratio. In order to reveal the molecular mechanism by which LET caused sex reversal, juvenile carp (120dph) were exposed to 0, 5, 25, 125, and 625μg/L LET for 15 days, and the expression profiles of seve...
Asad Abdullah1, Muhammad Irfan Ullah1,*, Muhammad Waqar Hassan2,    Samina Khalid3, Yasir Iftikhar4, Muhammad Arshad1 and Jaime Molina-Ochoa5,6
...ed at 6% NSE while at 2% concentration of NSE after 148 hrs, 39. 16% reduction was observed. While maximum reduction on non-Bt cotton at 6% recorded as 66.60% and minimum at 2% concentration recorded as 48.72% of neem seed extract against B. tabaci. In case of A. biguttula, maximum reduction in population was observed at 6% concentration of NSE (64.94%) and minimum at ...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...l acetate. The sublethal concentration of ethanol based extract of C. colocynthis increased larval and pupal duration as compared with control. The percent pupation, adult emergence and pupal weight decreased in treated populations as compared with control populations. The results of this study can be used in integrated pest management program for the management of H. armigera.
...
Nargis Bano and Khalid Mahmood Qureshi*
...lt suggests that SA at a concentration of 3mML-1 concentration could be used commercially to improve yield of strawberry.
...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1,  A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...xtender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm characteristics such as sper...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...tively. MgCl2 concentration did not affect and added at 2.0 mM. Four different culture methods were compared for their efficiency for avian mycoplasmas culture. The B method (parts of trachea) were found most efficient for producing growth within 36 h while method A produced within 48 h nevertheless both had the same positivity (70%). While other two methods C (filtrate into broth) and D (filtrate onto agar) produced just 40% and 31%. Moreover, the ...
Jiandong Wu, Miao Ye and Zaigui Wang*
...: for adsorption, sample concentration was 2.542 mg·mL-1, pH was 3.7, processing volume was 4 BV; flow rate was 2 BV·h-1; temperature was 25°C. For desorption, elution solvent ethanol-water (95%, v/v) solution was 4.5 BV; flow rate was 2.0 BV·h-1. Flavonoids with content of 68.3% were obtained with a flavonoid recovery of 93.7%. The results showed that AB-8 resin revealed a good ability to purify total ...

 Zeenat Bano1,*, Sajid Abdullah1, Waqas Ahmad2, Muhammad Anjum Zia3 and Wardah Hassan1

... from all the sites. The concentrations of Zinc (Zn), copper (Cu) and nickel (Ni) were higher in river water as compared to farm and hatchery. Different organs of fish collected from different sites showed variations in their metal concentration. In Indus River fish, the concentrations of metals were highest in liver followed by kidney and gills. The Indus River fish also exhibited ...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regulated TFF3 mRNA expression. Furthermore, TFF3 mRNA expression was decreased by rhTFF3 treatment. These findings indicate that TFF3 attenuates LPS-induced cell damage, and TFF3 mRNA expression is influenced by LPS and rhTFF3 in intestinal mucous epithelial cell...

Bibi Haleema1*, Abdur Rab1 and Syed Asghar Hussain2 

... calcium, boron and zinc concentration for enhancing the growth and fruit related attributes of tomato. The experiment was conducted using Randomized Complete Block (RCB) Design with 3 factors, replicated 3 times. Calcium (0, 0.3, 0.6 and 0.9%), Boron (0, 0.25, 0.5%) and Zinc (0, 0.25, 0.5%) were applied as foliar spray three times. Calcium application at 0.6% increased plant height (88.04 cm), number of primary (2.63) and secondary (7.15) branches, leaves pla...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...rphology, viability, and concentration. In conclusion, gradation of electrical stimulation into three stages (our modified Method II) could help to ease the collection of semen samples from bulls with minimum discomfort signs. Furthermore, the modified method is also recommended to use for other animals, in particular, the wild animals.
...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
...8 using direct smear and concentration methods. One hundred and fifteen (11.04%) participants were found infected with one or more than one intestinal protozoans. Forty one (35.6%) of the participants were infected with single parasite and seventy four (64.3%) with multiple infections. Entamoeba histolytica 30.5% (n=77/252), Giardia lamblia 15.0% (n=38/252), Ascaris lumbricoides 17% (n=43/252), Trichuris trichura 11.1% (n=28/252), <...
Faiza Ambreen*1 and Muhammad Javed2
...and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated and fish were exposed to these concentrations, separately in glass aquaria for 70 days at constant laboratory conditions. Peripheral blood erythrocytes were taken on 14, 28, 42, 56 and 70 day of exposure for th...

Naila Riaz1 , Hafiz Muhammad Tahir2* , Khizar Samiullah3

...topically with different concentrations of the crude venom. All aphids died within 6 hr with oral treatment at 1.5µl of the venom dose. However, at the same venom dose, 100% mortality was achieved at 8 hr posttreatment when applied topically. Similarly, oral administration of 1µl and 0.5µl venom caused 100% mortality at 8 hr post-treatment compared to 10 hr and 12 hr post-treatment of topical application, respectively. It is concluded that or...

Irfan1* , Arshad Javid2 , Muhammad Altaf3 , Muhammad Shahbaz3 , and Khalid Javed Iqbal4 

...ignificantly higher urea concentrations 33.45±3.39 mg/dL were recorded from the serum of the birds reared under free range rearing system. However, non-significant variations in albumin, total protein, ALP and AST were recorded for all the three rearing systems. Significantly higher values of cholesterol, globulin, albumin, creatinine and ALT were recorded from serum of male M. gallopavo while urea and ALP were higher in hens’ serum. However, non-...
Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1
...hree levels such as NaOH concentration (0.6, 0.8, 1.0%), substrate concentration (5, 10, 15%) and residence time (4, 6, 8h) with and without steam (autoclaving at 121oC for 15min and 15psi). After pretreatment, cellulase production was conducted in 250ml Erlenmeyer flask at 50 ºC, pH 5 with shaking speed of 120 rpm using 2% pretreated eucalyptus leaves as carbon source and 2% v/v inoculum size in submerged fermentation ...

Ayesha Aihetasham1*, Khalid Zamir Rasib2, Syeda Rida Hasan1, Imran Bodlah3

...al efficacy of different concentrations of aqueous leaf extracts of three medicinal plants viz., Carica papaya (paw paw), Helianthus annus (Sunflower) and Bougainvillea glabra (Paper flower) against Heterotermes indicola. The leaf extract of C. papaya caused highest mortality i.e. 100% of 10%, 5% and 3% concentration. Bougainvillea glabra and H. annus caused 100% mortality at 10% and 5% concentration...
Muhammad Iqbal Anjum*, Shahbaz Javaid and Mukhtar Ahmad Nadeem
...icks on R-II (1.89). The concentration of Ca and P were 10.60, 9.47 and 11.43 mg/dL and 5.76, 4.91 and 7.11 mg/dL in blood serum and 32.61, 31.36 and 32.83% and 16.57, 15.92 and 16.60% in tibia bone of chicks fed rations I, II and III, respectively. The total feed cost per unit weight gain of broilers fed R-III diet was numerically 14% and 9% less than the broilers fed R-II and R-I, respectively. Results suggested that exogenous microbial phytase supplementati...

Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3
 

... conditions of 0.6% acid concentration, 10% substrate concentration, and resident time of 4h. The maximum FPase activity was 2.015 1U/ml/min under conditions of 0.8% acid concentration, 10% substrate concentration, and time of 6h. This showed that peanut shells could successfully be used as substrate for cellulase production in submerged fermentation.

 Maryum Fakhar, Iqra Jabbar, Anjum Nasim Sabri*

... presence of nickel. Two concentrations of nickel were used i.e., 500μg/ml and 1000μg/ml for subsequent tests. The results revealed that at low/ sub-inhibitory concentration of nickel (500 μg/ml), motility is inhibited; adhesion, hydrophobicity, aggregation, EPS production and biofilm formation of two strains are increased. This biofilm forming ability can be employed in
urification of waste waters and treat...

 Asma Akhtar, Abdul Rehman

.... The minimum inhibitory concentration of both isolates showed fair growth up to 1 mM of K2Te2O3. Both strains showed maximum growth at 37ºC and pH of 7. The bacterial growth in the presence of metal ions was delayed as compared to the control. High ellurite reduction potential was shown 98% (1f) and 97% (2b) after 24 h of incubation. Glucose 6 phosphate dehydrogenase (G6PDH) activity was increased to 279% (1f) and 150% (2b) when ompared to the respective...
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
.../sup> of LC50 concentrations of iron chloride for 30 days in the glass aquaria with three replications for each treatment. After 30-day exposure of iron chloride, the fish organs viz. kidney and liver were isolated and the peroxidase enzyme activity was analyzed. Activity of peroxidase was assessed by measuring the conversion of guaiacol to tetraguaiacol, spectrophotometrically, at a wavelength of 470 nm. The peroxidase activity was measured ...

Farah Rauf Shakoori, Momina Shokat, Faiza Saleem and Tanzeela Riaz

...6;C, pH 9 using 1% xylan concentration, while XPB-CW01 produced maximum amount of xylanase at 50ᵒC, pH 7 with 1.5% xylan concentration. Maximum xylanase activity was achieved at temperature 50ᵒC, pH9 with 2% xylan concentration. Xylanase produced by XPB-GS02 was active from 35-80oC, while XPB-CW01 was active from 30 _70oC with optimum temperature 50°C.

...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
...8 using direct smear and concentration methods. Two hundred and twenty one (21.2%) participants were found infected with one or more than one intestinal tapeworms. Seventy seven (7.39%) of the participants were infected with single parasite and one hundred forty four (13.8%) with multiple infections. Taenia saginata 32.6% (n=146/447), Ascaris lumbricoides 20.3% (n=91/447), Hymenolepis nana 19.7% (n=88/447), Trichuris trichura 14.3% ...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

...plemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots ...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...wed decay in mean lutein concentration at higher temperatures i.e. 25oC and 45oC. The microbiological study of the extracted color and candies prepared in laboratory and dyed with T. erecta extract color showed that the color extracted from T.erecta itself do not promote microbial growth rather it has antibacterial activity. The lethal dose and toxicity determination of the color extracted from marigold showed that it is saf...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
...th ammunium sulphate, at concentrations as low as 30 μl. Optimized conditions for maximum yield of mutant SK included CSL 6%, 24 h. of fermentation, pH 7.5, 37oC, KH2PO4 0.04%, K2HPO4, NaHCO3 0.15% and 0.015% CH3COONa. The results indicated that UV is the most effective mutagen for hyper-production of SK. Mutant strain UV6 has the potential for enhanced SK production with commercia...

Ata-ul-Mohsin*, Ehsan-ul-Haq** and Muhammad Naeemullah*

...cium and high phosphorus concentrations applied to a partially resistant wheat cultivar “Rapier” did not have a significant effect on the growth and development of the aphid or its parasitoid. However, the mean fecundity of the parasitoid was higher on plants with high phosphorus than those treated with high calcium concentrations.

...

 Nasreen Sultana* and A.Ghaffar**

...cides at three different concentration viz., 1, 2, 3 g (a.i.) kg-1 seeds reduced the recovery of the seed borne fungi. The most effective seed treatments were Benlate, Topsin-M, Carbendazim and Aliette @ 3 g kg-1 seeds which enhanced seed germination and reduced seed infection in bottle gourd. Trichoderma harzianum, T. viride, Gliocladium virens, Stachybotrys atra and Bacillus subtilis showed better results in the control of pre- and post-emergence infection o...

 Ahmed Aziz Kurd, Amanullah, Saifullah Khan, Basharat Hussain Shah and Munir Ahmed Khetran*

...eased with increased IBA concentration, whereas, number of roots and root length increased with increased IBA concentration. It was concluded that to obtain the most roots per cutting and the longest root length, application of 4000 ppm IBA was the best and to obtain the most number of rooted cuttings, application of 3000 ppm IBA was the best.

...

 Rahila Nazli, Tehmina Sohail, Bushra Nawab and Zahra Yaqeen*

...idia sepium in different concentration was used to investigate its antimicrobial activity against gram +ve and gram-ve bacteria. The study was also extended to some species of fungi. Results showed that the activity was more pronounced against gram +ve organisms and fungi. Maximum inhibition activity was calculated against all the groups of organisms, which was between 0.5 and 1mg ml-1 against bacteria and 2.5 mg ml-1 against fungi. It is therefore concluded t...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...ded among all groups and concentration of red blood cells (RBCs), white blood cells (WBCs), hematocrit (HCT) and numbers of lymphocytes were highest in treatment # 1 with the mean values of 1.29×10ˆ12 L-1, 54.9×10ˆ9 L-1, 18% and 48.7×10ˆ9 L-1. Microbial load was also determined inside and outside of fish as highest microbial load was observed in control group fish on both media nutrient agar (NA...
Abul Hassan Faiz*, Fakhar-i-Abbas and Lariab Zahra Faiz
...otassium and phosphorous concentration. These significant differences in soil composition have long-term effects on ecosystem sustainability and productivity of vegetation.
...
Jaime Molina-Ochoa1, 2, 6,*, Edelmira Galindo-Velasco2, Ana María Rosales-Gutiérrez2, Martín González-Ramírez3, Roberto Lezama-Gutiérrez3, Wilberth Chan-Cupul3, Steven R. Skoda4, Muhammad Irfan Ullah5, Luis Jorge García-Márquez2 and  John E. Foster6
...pers. For each treatment concentration, 10 adult engorged ticks were placed in the Petri dishes, and each treatment was replicated four times; the experiment was replicated two times. Tick mortality was recorded daily for 9 days. Our EPNs were able to parasitize engorged ticks, and tick mortality ranged between 70-100% after 9-d post-exposure. The faster and higher tick mortality was obtained with the concentration of 2000 I...

 Arshad Ali, Muhammad Arshadullah, Syed Ishtiaq Hyder, Imdad Ali Mahmood and Badar-uz-Zaman*

RICE PRODUCTIVITY AND SOIL HEALTH AS AFFECTED BY WHEAT RESIDUE INCORPORATION ALONG WITH NITROGEN STARTER DOSE UNDER SALT-AFFECTED SOIL
...yzed for K, Ca and Na -1 concentration in grain and straw. Tillering, grains spike , 1000-grain weight and paddy yield significantly (P≤ 0.05) increased with different -1 levels of N doses. Maximum tillers plant (27.66), plant height (138.33 cm), -1 spike length (28.33 cm), and grains spike (175), 1000-grain weight (28.66 -1 -1 g) and paddy yield (4.25 t ha ) was harvested with 5 t ha wheat straw -1 incorporation plus 60 kg N ha . The paddy yield was 19% mo...

 Syed Ishtiaq Hyder, Muhammad Arshadullah, Arshad Ali and Imdad Ali Mahmood*

EFFECT OF BORON NUTRITION ON PADDY YIELD UNDER SALINESODIC SOILS
... growth, yield and ionic concentration of rice directly sown on raised beds under -1 saline sodic soils (ECe=5.32 dS m , pH=8.52 and SAR=18.87) at Soil Salinity Research Institute, Pindi Bhattian, district Hafizabad, Punjab Pakistan during 2009 and 2010. Treatments were arranged in RCBD with three replications. The crop was harvested at maturity. Data on tillering, -1 plant height, spike length, number of grains spike , 1000-grain weight, straw and paddy yield...
EVALUATION OF LEGUME HERBS NUTRITIVE VALUE AS A RUMINANT FEED AND NITROGEN SUPPLY ON SOIL IN WEST TIMOR, INDONESIA
...d that the highest -l NO concentration after planting was in CP (5.72 mg ), followed by CT 3 -l -l -l (4.21mg ), MB (3.44 mg ), and DL (2.83 mg ). The species of legume was obviously highly significant (P<0.01) to biomass production 90 days after planting. Moreover, dry matter (DM) and organic matter (OM) in vitro digestibility were statistically different among the species of legume (P<0.01). In DL the DM and OM were highest i.e., 74.84 % and 74.37%. Me...

 Arshad Ali, Imdad Ali Mahmood, Muhammad Salim, Muhammad Arshadullah* and Abdul Rasool Naseem**

GROWTH AND YIELD OF DIFFERENT BRASSICA GENOTYPES UNDER SALINE SODIC CONDITIONS
... of crop maturity. Ionic concentration in plant tissues and oil content in seeds were also determined. Comparatively more number of branches and pods per plant were produced by cultivar Dunkled closely followed by BARD-I while maximum seed yield (241.7 and -1 235.1 kg ha ) was obtained from Dunkled and Sultan Raya, respectively which was statistically at par. However, BRS-II and Rainbow showed significantly more percent oil contents in their seeds but genotype...

 Muhammad Arshadullah, Syed Ishtiaq Hyder, Arshad Ali and Imdad Ali Mahmood*

CUMULATIVE EFFECT OF SULFUR AND CALCIUM ON WHEAT GROWTH AND YIELD UNDER SALINE-SODIC SOILS
...psum on growth and ionic concentration of -1 wheat variety (Saher) sown in saline-sodic soil (ECe=5.32 dS m , pH=8.52 and SAR=18.87) at Soil Salinity Research Institute (SSRI) Farm, Pindi Bhattian during rabi 2009-10. Treatments were arranged using randomized complete block design (RCBD) with three replications. The crop was harvested at maturity, data on tillering, plant height, spike -1 length, number of grains spike , 1000-grain weight, straw and paddy yiel...

 Abdul Rehman Roonjho*, Waseem A. Gillani**, Awais Rasool**, Naheed Akhtar**, Tariq Mahmood**, Arsalan A.***, M. Afzal*, Iqbal Khan* , M. Asghar Ranjha**** , M. Irfan**** and Javed Khan**

REPELLENCY EFFECTS OF DIFFERENT PLANT EXTRACTS TO COTTON MEALY BUG, TINSLEY (HEMIPTERA: PSEUDOCOCCIDAE)
...ol were evaluated at the concentration of 1000, 500 and 250 ppm against cotton mealy bug ( ) in a free choice bioassay for two weeks. The ethanol extract of was the most effective against cotton mealy bug having highest repellency (72.5%) at 500 ppm dose. The lowest average repellency (26.3 %) was observed in acetone extract of at 500 ppm dose.

...

 Adnan Umair*, Safdar Ali**, Muhammad Sarwar***, Kashif Bashir**, Muhammad Javed Tareen**** and Muhammad Asghar Malik*****

ASSESSMENT OF SOME PRIMING TECHNIQUES IN MUNGBEAN (VIGNA RADIATA) : A GREEN HOUSE STUDY
...lso improved the protein concentration at the early stage of seedlings. Phosphorous application through priming significantly improved germination up to 95%, seedling -1 vigour index up to 23.05 and protein content up to 2.17 (g FW).

...

 Said Salman*, Shah Jehan Khan*, Javed Khan**, Rehmat Ullah Khan*** and Imran Khan****

GENETIC VARIABILITY STUDIES IN BREAD WHEAT (TRITICUM AESTIVUM L.) ACCESSIONS
...eters except chlorophyll concentration index and number of spikelet per spike indicating considerable amount of variation among the accessions for each trait. The genotypic and phenotypic coefficient of variation estimates were higher for all the traits except chlorophyll concentration index and days to physiological maturity. Highest heritability estimates and expected genetic advance were found for all the traits except ch...

 Nazakat Nawaz*, Malik Shah Nawaz*, Mubashir Ahmad Khan*, Mirza M. Yasin*, Doulat Baig*, Nasir Mehmood Cheema**, Muhammad Amjad** and M. Altaf Sher**

EFFECT OF BORON ON PEANUT GENOTYPES UNDER RAINFED CONDITIONS
...equirements and critical concentrations in diagnostic parts of three peanut genotypes, BARD-479, ICG-7326 and ICGV-92023. These genotypes were grown in boron deficient soil at National Agricultural Research -1 Centre, Islamabad, Pakistan. Five rates 0.0, 0.5, 1.0, 2.0 and 4.0 kg B ha as boric acid (H BO ) were applied. Boron fertilizer applications significantly 3 3 increased dry pod yield of all the tested genotypes over control. Dry pod yields of BARD-479, I...

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...etween strains and among concentrations. Abamectin, at LC and LC , changed the activities 10 20 /levels of TE, CE, AcP, total protein and FAA in the larvae of both the strains. The activities of alpha-amylase, glucoamylase and AkP remained non-significant at both doses in the two strains. In PAK strain larvae, the TE activity was inhibited with depletion of total protein contents and elevation of FAA contents. In FSS-II larvae, the effect of abamectin on level...

Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***

ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...s were cytotoxic. As the concentration decreased, cytotoxicity as well as antiviral activities were lost. Based on these findings, it was concluded that GZ-08™ sanitizer or spray can be used as antiviral agent to clean or disinfect some non-living surfaces against different viruses in general and NDV in particular. However, in-vivo use of GZ-08™ in poultry against NDV is recommended only as pre-treatment with ND vaccines as it significantly reduced...

 Azhar Usman Ali*, Ghulam Sarwar*, Muhammad Aftab* and Sher Muhammad**

EFFECT OF SOIL AND FOLIAR APPLIED COPPER ON GROWTH AND YIELD OF WHEAT (TRITICUM AESTIVUM L.)
... and analyzed for copper concentration by using wet digestion method. Results of the experiment indicated that soil application of copper proved better than foliar application in increasing the yield and yield components of wheat. Similarly, foliar application -1 -1 of copper at 4mg kg (T ; level 2) was better than 2 mg kg (T ; level 1). 

...

 Muhammad Mudasar Aslam ⃰, Muhammad Jamil**, Ijaz Malook**, Amana Khatoon*, Ali Rehman*, Abdur Rahim***, Pirzada Khan*, Shakir Ullah Khan Shakir*, Shahid Irfan*, Faizan Ullah****, Khair Ul Bashar**, Mahideen Afridi** and Shafiq Ur Rehman*

PHYTOTOXIC EFFECTS OF CALOTROPIS PROCERA, TAMARIX APHYLLA AND PEGANUM HARMALA ON PLANT GROWTH OF WHEAT AND MUSTARD
... aqueous extracts at all concentrations (5%, 10%, 15%, 20% and 25%) but shoots were inhibited by higher concentrations (20% and 25%). This study revealed that wheat is more sensitive to different plant extracts as compared to mustard. It is thus concluded that inhibitory effect increases with the increase of extracts concentration.

...

 Abdul Rehman Khan*, Muhammad Mahmood–Ul-Hassan**, Rizwan Ahmad** and Anjum Munir ***

ARSENIC III TOLERANCE POTENTIAL OF FUNGI ISOLATED FROM POLLUTED AND NON-POLLUTED SOILS OF PAKISTAN
...m amended with different concentrations of As (50 to 5600 mg/L). The fungal tolerance was evaluated by comparing tolerance index (TI), minimum inhibitory concentrations (MIC) and growth rate. Out of 18 isolates, 12 belonged to genus , 3 to , 2 to and one to . The isolates and appeared to be most tolerant to arsenic. Fungal strains isolated from Gujranwala soil exhibited more As(III) tolerance than those from Multan and Islam...

 Asif ullah Khan*, Faizan Ullah*, Sultan Mehmood*, Muhammad Irshad* and Farhat Ullah Khan**

ALLELOPATHIC EFFECTS OF JATROPHA CURCAS L. LEAF AQUEOUS EXTRACT ON EARLY SEEDLING GROWTH OF L. PARTHENIUM HYSTEROPHORUS
.../ gm of extract). Higher concentration of the extract (25%) significantly decreased seed germination (%), seed germination index, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, seedling relative water content and seed vigor index. It was inferred from findings of the present investigation that aqueous extract of exhibits inhibitory effects on growth and development of and therefore could be a potential source of botanica...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
..., Huwa-San TR50 caused a concentration dependent reduction in the percentage of egg hatching. The percentages of larvae hatching from eggs of A. lycopersici were 73.42, 56.92, 25.41 and 17.97 under greenhouse conditions and 68, 53.14, 23.51 and 15.18 under laboratory conditions at 1000, 2000, 3000 and 4000ppm of Huwa-San TR50 after one week of treatment compared with the control (well water), respectively. The application of Huwa-San TR50 showed a promi...
Filiz Kutluyer* 
...tative changes different concentrations of L-tryptophan. In addition, L-tryptophan can be used in activation medium for O. mykiss, S. rizeensis and S. coruhensis.
...
Fatmagün Aydin1,* and Fatma Burcu Harmantepe2
...d cell count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total white blood cell count and differential leukocyte counts) showed no significant (P>0.05) differences between the control and all other treatments. Feed intake, specific growth rate, weight gain and final weight were decreased significantly with the increase of sage oil ...
Asmatullah1, Chaman Ara1,*, Shagufta Andleeb2, Maha Tahir1, Beenish Zahid1 and Madeeha Arshad1
...rpose, different aqueous concentrations of cadmium chloride i.e. 0.00, 6.25, 12.50, 25.00 µg/g B.W of mice were given orally to pregnant mice on days 6-12 of gestation daily. Another series of mice were treated with different concentrations of cadmium chloride and also with an antidote (guava fruit extract) on days 6-18 of gestation daily once a day. Fetuses were recovered on day 18 of gestation, analyzed based ...
Hao Zhang,1,2,3,* Hai Tao Nie,3 Tie Wei Ma,3 Zi Yu Wang3 and Feng Wang3
... to estimate the mineral concentrations and distributions in the main body tissues and the net requirements for maintenance and growth of F1Dorper × Hu ewe lambs. Thirty-five ewe lambs averaging 33.52±0.56 kg body weight (BW) were used. Seven ewe lambs were randomly chosen and slaughtered at 34.93 ± 0.37 kg BW as the baseline group for measuring initial body composition. Another seven lambs were also randomly chosen and offered a...
Hongwei Liang1,2,*, Miaomiao Tong1, Lihuan Cao1,2, Xiang Li3, Zhong Li1 and Guiwei Zou1,*
...red. Calipash had higher concentrations of flavor amino acids than muscle. Muscle tissue of the Huaihe River strain had the highest concentration of pharmacodynamic amino acids and the highest concentration (P < 0.05) of arachidonic acid (females: 109.83 mg/ 100g, males: 110.72 mg/100 g). Concentrations of eicosapentaenoic acid and docosahexaen...
Shireen Ihsan Izzaddeen1 and Ali Kaygısız2,*
...albumin, GH and globulin concentrations did not differ between the groups (P>0.05). Essential laurel oil containing whole milk, on the other hand, lowered serum triglyceride concentration (P<0.05). Altogether these results suggested that the consumption of laurel essential oil (600 mg/day) containing whole milk by calves might have positive influences on their health parameters compared to the control group.
...
Nilgün Paksoy1, Hikmet Dinç2 and Serap Kılıç Altun3,*
...udy was to determine the concentrations of Na, Mg, P, K, Ca, Fe, Cu, Zn, Ni, Cd, V and Ba in donkey, goat, and sheep milk samples. Fifty-six individual milk samples were collected from 17 lactating donkeys, 19 goats, and 20 sheeps in three different Turkish Farms. The samples were analyzed by inductively coupled plasma - mass spectrometry (ICP-MS). Minimum and maximum levels of Na, Mg, P, K, Ca, Fe, Cu, Zn in donkey milk samples were 108.6-202.7, 48.6-76, 355-...

Muhammad Jamal Khan1, Abdul Malik2*, Muhammad Shahzad Khattak2, Naveedullah1, Naeem Ijaz3 and Ishaq Ahmad4 

...linity having low Sodium concentration and may be suitable to salt tolerant crop. Regular assessment of water resource and constant monitoring of water quality is recommended. An appropriate measure may be adopted for recharge of the ground water aquifer through development of small dams in the catchments plain. 

...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
... optimal Co2+ concentration for the maximal activity of AMYSBS. Presence of ionic (SDS) and nonionic (Tween-20, TritonX-100) detergents showed an enhancing effect on the activity of AMYSBS. Stability studies of AMYSBS exhibited that enzyme was quiet stable at 55 °C. Kinetic studies demonstrated the Km and Vmax values of 6.67mg/ml and 2500µmol min-1 mg-1, respectiv...

Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4 

...ose, cortisol and HSP-70 concentrations wasmaximum in HF (43.44, 119.06 and72.11%) andcrossbred (38.66, 159.61 and79.12%) and minimum inAchai (34.93, 123.59 and52.53%) and Sahiwal (27.26, 117.22 and65.41%)respectively (P< 0.01). Holstein Frisian appeared as the most heat sensitive breed exhibiting changes at 32 °C i.e. 5 °C higher than 27 °C (upper critical temperature, UCT). Sahiwal and Achai were the most heat tolerant breeds manifesting chang...

Nadia Mubarik1, Aqib Iqbal1*, Iqbal Munir1 and Muhammad Arif2 

... water stress) and three concentrations of CaCl2 (5, 10 and 15 mM CaCl2.2H2O) as foliar spray on maize seedlings 20 days after emergence (DAE). Water was sprayed as untreated control on maize seedlings 20 DAE. After foliar application, the plants were regularly irrigated upto one week and water stress was imposed by withholding water from half of the pots for 20 days. Data were recorded on the relative water content (RWC), proline, sugar content and transcript...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...nificantly increased the concentration of tumor necrosis factor-α (TNF-α) in bursa of Fabricius (P<0.05), along with an increase in the concentrations of immunoglobulin-G (IgG) and interleukin-12 (IL-12) in the serum of ducks at 1 day and 7 days after LPS injection (P<0.05). However, there were no apparent changes in organ index or the relative expression of TLR4 and IgM (P>0.05). Fu...
Sherin Reda Rouby
...ds that reflect the high concentrations of the virus. The results of the current study confirm the suitability of RP030 gene in differentiation of lumpy skin disease virus and sheep poxvirus in a single PCR assay without the necessity of post processing steps. 
...
Shagufta Andleeb*1, Fariha Zahid1, Noor-ul-Ain1, Yousra Nisar1, Chaman Ara2
...mesticus). Different concentrations of permethrin (0, 5, 10 and 20ppm) were prepared in sterilized distilled water. These doses were injected into eggs on 3rd day while recovery of embryos was done on 7th day of incubation. Disrupted embryos with microcephly, hydrocephaly, short neck, micromelia, amelia, micrognathia, agnathia, cataract, ectopiacordis, omphalocoel, axis distortion, anencephaly, anophthalmia, microphthalmia, phocomelia...
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
...evels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase production was achieved by solid substrate which was conducted in 250mL capacity Erlenmeyer flask in which incubation of Bacillus subtilis was carried out for 24 h of fermentation period at temperature of 50oC. Results showed that cellulase production was greatly affected by ...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
... SFF supplemented at 10% concentration showed a significant increase in blastocyst development when compared with other groups. Gene expression analysis revealed a significant increase in BAX expression in groups supplemented with SFF 20% and 40%, whereas the expression of BCL-2 was significantly increased only in the 40% SFF supplemented group. The ratio of Bax to Bcl-2 was significantly increased in the embryos derived from oocytes matured in 20% and 40% SFF...
Felicia NkechiEkeh*, Ifeanyi Oscar Ndimkaoha Aguzie, Joy Ihuoma Nzei, Chinenye Maria-Goretti Ohanu, Godwin Ikechukwu Ngwu and ChukwudiebubeUgolo
...wo extracts in different concentrations, a total of 63 sundried adult C. gariepinus of mean weight 111.47 ± 2.34g were randomized into three treatment groups A – C for each extract and a control D. The treatment groups were in triplicates with each containing three catfish. The groups contained fish infested with 20 larvae, 20 adults and eggs of D. maculatus. Groups A – C were exposed to 8, 12 and 15% ( w/w) of extract per kilo...
Hafiz Azhar Ali Khan1,*, Waseem Akram2, Sumi Lee3, Shakir Manzoor1, Syed Rajab Ayub1, Khalil Ur Rehman1, Shinawar Waseem Ali1, Muhammad Bilal Chattha1 and Sumaira Maqsood1
.... Based on median lethal concentration (LC50) values, all the strains of S. oryzae and R. dominica were more susceptible to spinosad than T. castaneum strains. The resistance ratios (RRs) of field strains at LC50 values were in the range of 2.24 to 3.24 fold for T. castaneum, 3.33 to 9.00 fold for R. dominica, and 1.73 to 3.45 fold for S. oryzae. The results revealed a variation in susceptibility ...

Feroza Haider1,2, Shamim Gul2,3*, Javaid Hussain4, Sadaf Asalam Ghori5, Muhammad Naeem Shahwani6, Muhammad Murad6 and Abdul Manan Kakar

...educed significantly the concentration of iron and zinc in roots of B. rapa. 

...

Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Muhammad Jamal Khan1, Zahir Shah1 and Farmanullah Khan1 

...various nutrients uptake/concentration. The data revealed that all strains of cyanobacteria had significantly (p < 0.05) improved the nutrients up-take/concentration and agronomic traits of rice crop as compared to control. Among the strains, the Gloeobacter strain (MMF-4) surpassed by augmenting all agronomic parameters of rice crop like plant height (85 cm), plant fresh weight (45 g), dry weight (24.5 g) and root dry we...

Gulsanga1, Zahin Anjum1, Sahib Alam2* and Farhat Shehzad

...d that Cu, Zn, Pb and Ni concentrations in cow meat ranged from 7.27 to 14.81, 64.00 to 112.12, 3.30 to 6.25 and 0.73 to 2.53 mg/kg whereas the concentrations of these heavy metals in buffalo meat varied from 3.15 to 7.71, 72.88 to 88.47, 1.41 to 13.28 and 0.38 to 1.93 mg/kg, respectively. The average Na, K, Ca, Mg and P contents in cow’s and buffalo’s meat were found to be 54.75 and 67.45, 351.67 and 333.75, 4.6...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
... IPTG induction to final concentration 0.5 mM at 37oC for 4 h and purified using affinity chromatography. SUMO tag was removed from SUMO-IFNα2-Tα1 by SUMO protease and recombinant IFNα2-Tα1 was collected in flow through by affinity chromatography. The MW (~23 kDa) of IFNα2-Tα1 was determined by 12 % SDS-PAGE and its integrity was confirmed by Immuno blot analysis using anti-interferon α-2 and anti-thymosin &...
Huma Naz1,*, Sajid Abdullah2, Sidra Naz3, Sidra Abbas2, Wardah Hassan2, Shakeela Perveen2, Moazama Batool1, Laiba Shafique2
...50 and lethal concentration of Fe+Ni+Pb+Zn mixture for the fish, Cirrhina mrigala was determined in this research work. The fish were exposed to each of the 19 different concentrations (5-90 mg/L) of Fe+Zn+Pb+Ni mixture to determine 96 h LC50 and lethal concentration. During acute toxicity test, behavioral changes and catalase activity in liver, kidney, gills, ...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...olism and macromolecular concentrations of 4th and 6th instar larvae of a stored product pest, Trogoderma granarium. The LC50 values of λ-cyhalothrin for 4th and 6th instar larvae of Lahore population was 15.93 and 13.76ppm respectively, while 19.07 and 16.21ppm were for the 4th and 6th instar larvae of Gujranwala population, respectively. The resistance ratio RR (0.67 a...

Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1* 

...The influence of varying concentrations of both EOs was determined in vitro using the agar dilution test. Concentration as low as 0.25% and 0.125% of thyme and clove oils, respectively, were able to completely inhibit the growth of CP. An in vivo study was conducted in commercial broiler chickens, where, 2 groups of 10 day-old broilers were fed a balanced ration with addition of thyme (0.25%) or clove (0.125%). Birds were th...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
...n. In the present study, concentration levels of seven heavy metals were measured in different body parts of Baladi chicken from Jazan region, Saudi Arabia using Inductively Coupled-Plasma Optical Emission Spectrometry (ICP-OES). The concentrations of heavy metals in various chicken parts were recorded to be in the range of nd-0.01, 0.07-0.18, 1.08-3.95, 9.61-119.0, nd-0.16, 0.13-1.10, and 8.63-67.58 mg/kg for Cd, Cr, Cu, Fe...
Wael S. El-Tohamy1,*, Russell R. Hopcroft2 and Nagwa E.M. Abdel Aziz3
..., temperature, phosphate concentration and phytoplankton biomass. The main spatial gradients along the estuary were associated with salinity. The high salinity zone in the estuary downstream was dominated by the calanoid paracalanidae and the harpacticoid Euterpina acutifrons. In the lower salinity transects, the tintinnid Favella serrata dominated the estuary midstream, together with several rotifer species in the estuary upstream. The juvenile ...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
... higher serum GH and PRL concentration in comparison with T2 and control groups (p < 0.05). Expectantly, mice in T1 group also showed significantly higher average weight gain and offspring weaning weight than that in T2 and control groups, respectively (p < 0.05). Overall, these results suggested that novel somatostatin plasmid fused to tPA signal peptide obtained stronger immunogenicity, and better potentials of promoting growth and...
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
... to determine the lethal concentrations of this virus against the first and second larval instar of S. exigua. The potency of ten plant extracts as UV absorbers were done by measuring the absorption spectra of the 0.5% extracts using UV spectrophotometer. Based on preliminary data, clove, green tea and henna extracts at 10% (v/v) concentration were added to the virus and tested under semi-field conditions on the cabba...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...y treated with different concentration of EMS and MMS. Among 13 mutant variants, EM-7 showing maximum lipase activity of 71±3.55 U/g, was selected. Resistance in the hyper producing strain was developed by exposing it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optimum conditions at 30°C temperature, pH 7, and incubation period 64 h, the lipase activity was increased up to 124±2.48 U/g. The cru...

Wasim Javaid1, Farid Asif Shaheen1*, Farah Naz2 and Muhammad Usman Raja2 

...y using different fungal concentrations of 1×106, 1×107 and 1×108 spores/ml in stored chickpea grains. Highest mortality (90%) of PB was observed in 72 hours through application of 1×108spores/ml of M. anisopliae compared to B. bassiana with 60% mortality. On 24 hours of exposure, minimum mortality (4%) in PB was recorded in grains treated with 1×106 spores/ml of B. bassiana. In M. anisopliae, the concentr...
Muhammad Anwar Iqbal1, Habib Rehman2, Ali Hussain3,*, Faiza Jabeen4, Ijaz Ahmad2, Kamran Ashraf5, Muhammad Irshad Arshad6 and Omaima Khan7
...ineral levels and caecal concentrations of E. coli and Clostridium spp. in Japanese quail (Coturnix coturnix japonica). Japanese quail (one-day old; n = 1320) were taken and randomly separated into four groups (n = 320), having 8 replicates (n = 40) in each group. Corn-based poultry feed (basal diet) was given to control group birds (Group A) and the same feed mixed with three different concentrations of...
S. Jalbani1, N. T. Narejo2,*, P. Khan2 and Y.M. Jalbani3
...ocytes count, hemoglobin concentration and erythrocyte sedimentation rate of the two sexes. Male fish had total erythrocytes 5.05 ± 0.5 × 106 and hemoglobin content 11.5 ± 1.5 g/in ml compared to 4.04 ± 0.5 × 106 and 7.3 ± 1.3 g/in ml in the female. The female fish had significantly higher values of ESR in comparison of male. It may be due to different plasma viscosity and specific gravity of the er...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
... mice fetuses. Different concentrations of the drug, 0.00, 4.10, 8.33 and 16.66 µg/g B.W. were administered orally to the dams on days 6-12 of gestation, and fetuses were recovered on day 18. Morphological observations of fetuses from 4.10, 8.33 and 16.66 µg/g B.W. dose groups showed abnormalities such as skin hemorrhages, microphthalmia, limb deformities, hygromas, kyphosis, curved and short tail and fluid filled abdominal cysts. Significant incre...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
...t in the camel milk. The concentration of immunoglobin was found higher in camel and goat milks as compared to other species. Moreover, the strength and mobility of bovine serum albumin (BSA) was different in all milk species.
...

Abdur Rehman and Zahir Shah 

...t of nodules by 135 %, N concentration in plant by 29 %, N uptake by 50 %, N2 fixation by 85 % over the control. Application of Mo and Fe at all levels alone or in combinations also improved soil organic matter, mineral N, P and K status that could be associated with more N2 fixation, enhanced plant and root growths of plants. These results suggested that application of both Mo and Fe is necessary for pea crop enhanced nodulation, N2 fixation, yield, nutrient ...
Kamran Khan1, Sarzamin Khan2, Nazir Ahmad Khan2,* and Naseer Ahmad2
...antify the effect of two concentrations and technological forms of flaxseed supplementation on growth performance, carcass yield and meat quality of rabbits. Sixty indigenous rabbits were divided randomly into five treatment groups. The control group was fed on standard concentrate basal diet, whereas in the treated groups, the basal diet was supplemented with low (3.5%; LCF) or high (7%; HCF) level of crushed flaxseed and low (1.5%; LFO) or high (3%; HFO) lev...

Fiaz Ahmad1, Mumtaz Hussain2, Ghulam Abbas1, Muhammad Jawad Asghar1 and Muhammad Rizwan3* 

... to three different Pb2+ concentrations under two water stress conditions. Different morpho-physiological and yield traits were studied. Both varieties showed gradual reduction in morphological traits at increasing concentration of lead and water stress. Moreover, morphological traits were more severely affected by combined lead and water stress treatment (80 mg Pb2+ 50% FC) as compared to single lead (40 mg Pb2+ 100% FC and...
Md. Abu Affan1,*, Abdulmohsin Al-Sofyani1, Adnan Jaman Turki2 and Mohammed S.B. Hariri3
...inity and nearly zero DO concentration might be the reasons of killing fish by creating osmotically imbalance and suffocation of fishes. Additionally, due to low pH, huge mucous covered the gills filaments which might have created difficulties for respiration. Thus, the fish killing incident might have occurred by synergetic effects of different physico-chemical factors.
...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...nc and ferric oxide at a concentration of 12, 200 and 1µg/ml, respectively. This study may help in the development of chemotherapeutic methods against multidrug resistant bacterial pathogens in chronic and acute lung infections. It provides a practical approach towards the use of nanoparticles to enhance antimicrobial activity against these pathogens.
...
Habib-ur-Rehman1*, Saima Mirza1*, Mansoor-ul-Hasan2, Qurban Ali3, Hafiz Abdullah Shakir4, Muhammad Yasir5
...th micropipette at three concentrations (5, 10 and15%) of each of the plant extract. The repellence was recorded after 24, 48 and 72 h of the treatments application. The findings of experimental trials presented significant mean repellency 79.15% of T. castaneum (with methanolic extract of C. paradise)followed by 76.21% (with R. communis extract)and 63.36% (in J.curcus extract) at 15% concentra...
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...olism and macromolecular concentrations of 4th and 6th instar larvae of a stored grain pest, Trogoderma granarium. The LC50 values of esfenvalerate for 4th and 6th instar larvae of Phosphine and deltamethrin-susceptible population was 34.29 and 28.05ppm, respectively, while 39.30 and 32.67ppm were for the 4th and 6th instar larvae of Phosphine and deltamethrin-tolerant populati...

Muhammad Zahid1, Naeem Iqbal1, Sohaib Muhammad2*, Summiya Faisal3, Wajid Mahboob3, Makhdoom Hussain4 and Zaheer ud din Khan2 

... was supplied in various concentrations (0, 5, 10, 25 and 50 mM) as seed priming and foliar spray treatments at vegetative and reproductive stages. Plant growth and grain yields were reduced under drought. Two glucose treatments (10 mM and 50 mM) improved tillering and dry biomass as compared to other treatments. Foliar sprayed-glucose treated plants showed increasing trends in grain yield under drought. Physio-chemical attributes were also modulated by exogen...
Nevran Eylem Akman Gündüz1,* and Özgür Özcan2
...oration assay. Different concentrations (2, 5, 10, 50, 100, 200, 500 and 1000 mg/L) of GA3 were added to the synthetic diet and protein, lipid, carbohydrate and glycogen levels in the hemolymph were evaluated for the GA3 concentrations. The hemolymph protein level of the larvae increased significantly at 5, 10, 50 and 200 mg/L with respect to the control group. The lipid level of hemolymph fluctuated am...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...ied by testing different concentrations (1 x 102 POB (Polyhedral occlusion bodies) mL-1- 1 x 1010 POB mL-1) from the occlusion bodies produced from NPV isolates against 2nd, 3rd, 4th and 5th instar larvae of S. litura. The rapid and sensitive polymerase chain reaction (PCR) technique was used for the molecular detection of NPV gene from native NPV diseased insect. Mu...
Xiaqing Chen, Yü Huang and Zhen Huang*
...azim was affected by the concentration of each component in the mixtures. The synergistic effects were observed in treatments T4 (3.5+40.0), T5 (7.0+20.0) and T6 (7.0+40.0), respectively. The treatment T3 (3.5+20.0) was determined as additive effects according to their cumulative adjusted mortalities, Me and Chi-square values. The current research on the joint action of ethylacetate extracts from T. hamatum with carbendazim offered a promising, safe and...
Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
... directly related to the concentration of each component used in mixtures. Additive effects were observed in all treatments according to the mycelial growth inhibition, Me and Chi-square values. The use of T. hamatum offers a promising and effective co-formulation product or alternative to chemical fungicides in controlling popcorn disease of mulberry.
...

Ali Sher1*, Khalid Naveed1, Gulzar Ahmad2, Ayub Khan1, Muhammad Saeed1 and Shah Masaud1 

... and 150 kg ha-1), three concentrations of Zn and Fe (i.e. 1, 2, 3 kg ha-1) each along with two controls i.e. no micro nutrients no water (control-I) and no micro nutrients + water (control-II). The N was soil applied and MN as foliar spray at booting stage. Days to anthesis, leaf area tiller-1, leaf area index, plant height, days to physiological maturity and biological yield were significantly increased by both N and Zn while the same parameters except days ...

Safina Naz1, Muhammad Akbar Anjum1, Saeed Akhtar2, Syed Atif Hasan Naqvi3* and Muhammad Asif Zulfiqar

...sewage water had greater concentrations of these metals, which were higher than the safe limits recommended by WHO (1996). All the vegetables grown with tubewell water had significantly lower heavy metal contents, well below the critical limits. The concentration of Pb in tomato and cauliflower, Cd in tomato and spinach, and Fe in spinach irrigated with canal water were also detected beyond the safe limits. The study conclud...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
...re exposed to sub lethal concentrations of deltamethrine. Fish were divided into 4 groups and each group contains six fish. Experimental groups viz. A, B and C were exposed to 25, 50 and 75% of LC50, respectively for four days. Blood samples for biochemical analysis (blood glucose, cholesterol and total protein) and gills for histology were taken after 96 hrs. After acute exposure to pesticide, significant changes were observed in serum bioch...

Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...re exposed to sub-lethal concentration (1/3rd of LC50) of DM+END mixture for 14-day. CAT was partially purified by using ammonium sulphate precipitation technique and kinetic characterization was also performed against different pH and temperature. CAT activity was measured spectrophotometrically at A240 nm. Results showed that highest CAT activity was noted from the crude extract of control fish gills (140.66±0.71UmL-1) as compared to DM+END exposed fi...

Raza Ullah Khan*, Ahmad Khan, Mohammad Zameer Khan, Fayyaz Hussain and Sonia Saba 

... selected micronutrients concentration in shoot and root were determined once after harvest of crop and shown as mean of three replications. Results showed that different combination of P yielded significant response in plant growth parameter. It was found that split application of P equally as soil and foliar caused an increase in biomass and grain yield. With respect to post harvest P and K concentration in plant parts, al...

Amar Razzaq1, Abdur Rehman4*, Abdul Hassan Qureshi2, Iqbal Javed3, Raheel Saqib5 and Muhammad Nadeem Iqbal6 

...sed on relatively higher concentration of HEI infrastructures installed on the farms. Sprinkler irrigation system was mainly installed on wheat crop while the drip irrigation systems were installed on mango orchards. Therefore, one half of the sample consisted of modern and conventional farmers growing wheat crop and the other half of the sample consisted of modern and conventional farmers growing mango orchards. Economic analysis measures of benefit-cost rati...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...consequences at elevated concentrations. The bacterial cell utilizes a battery of copper detoxification and exclusion mechanisms of which multicopper oxidase (CueO) is an integral component. In addition to acting as copper regulatory elements, CueOs have been shown to possess laccase activity. In this study, we have cloned and over-expressed the CueO of locally isolated Klebsiella pneumoniae KW strain. The CueO protein was purified to homogeneity b...
Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...osine triphosphate (ATP) concentration, as well as nitric oxide synthase (NOS) activity and nitric oxide (NO) concentration catalyzed by NOS. Based on biochemical analysis of the above, results showed that O2- production could be induced significantly after cold stress at 15°C and heat stress above 30°C. SOD activity showed a similar changing profile as the concentration...

Ali Muhammad*, Yasser Durrani, Majid Suhail Hashmi, Ihsan Mabood Qazi, Muhammad Ayub and Saifullah 

Sabahat Noor*1, Shaukat Ali1, Hafeez-ur-Rahman1, Farhatullah2 and Ghulam Muhammad Ali1 

...different levels of salt concentrations separately. Four parameters i.e. sugar contents, proline contents, chlorophyll contents and relative water contents were recorded and compared in transformed and non-transformed plants. It was observed that under drought and salt stresses, proline contents, sugar contents, chlorophyll contents and relative water contents (RWC) were significantly higher in transgenic plants as compared to non-transgenic plants. From obtai...

Mohsin Iqbal1, Farid Asif Shaheen1*, Farah Naz2, Muhammad Usman Raja2, Muhammad Fiaz3 and Muhammad Nadeem1 

...treated with the highest concentration of B. bassiana as compared to that of M. anisopliae. These non-chemical control strategies will have significant contribution towards development of commercial formulations of bio-pesticides to be used against this grain beetle and other stored product insect pests. 

...

Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1 

...ading capacity at higher concentration of calcium chloride i.e. 0.5 M. Antimicrobial assay clearly indicated that pumpkin seed oil was very efficient against S. aureus, which is a gram positive bacteria. Pumpkin seed oil and control Streptomycin had almost same inhibitory zone that showed its effective use in food. In general, results showed that encapsulation is an effective approach for protection of active ingredients of pumpkin seed oil. 

...
Ghazunfar Rashid1, Muhammad Avais1, Syed Saleem Ahmad1, Muhammad Hassan Mushtaq2, Rais Ahmed3,*, Mahboob Ali1, Muhammad Naveed-ul-Haque4, Mehtab Ahmad5Mumtaz Ali Khan1 and Naimat Ullah Khan6
...i, Bajra and Sarson. The concentrations were lower in the afternoon in the leaves and in mature crops as compared to stem parts, immature plants, and in samples collected from plants during morning hours. The nitrate concentration was lower in samples collected from Ravi area, as compared to samples collected from villages of Pattoki and Okara. Blood samples were collected from animals feeding on above fodders; Spectrophotom...

Jafar Aqeel Naqi1, Abdul Mateen1, Dilawar Hussain2,*, Hafiz Muhammad Tahir2, Sajjad Hussain1 and Abuzar Tabasum1 

...n corpuscular hemoglobin concentration (MCHC). Average mean weight gain (11.03±0.8g) and average mean length (7.8±0.4cm) was observed maximum in T4 as compared to other dietary treatments. There observed a significant difference (P<0.05) in FCR among all the dietary treatments with highest in T4 (0.59±0.32). Dietary treatment T5 (10/500g) showed significance difference (P<0.05) in red blood cells (RBC...
Sajid Ali Khan1, Mazhar Hussain Ranjha1, Azhar Abbas Khan2,*, Muhammad Sagheer1, Amjad Abbas1 and Zeshan Hassan2
... solvent. Four different concentrations 5%, 10%, 15% and 20% were prepared by diluting in acetone. The data regarding mortality, growth regulation and repellency of T. granarium was observed. Repellency of plant extracts was tested using area preference method while for the growth regulatory potential of plant extracts, the adults insects were released in wheat grains treated with different concentrations of plant ext...

Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1 

...n were tested at various concentrations under in vitro conditions by poison food and disk diffusion techniques while best effective seven were trialed in glass house and field experiments to determine their efficacy against Xanthomonas oryzae pv. oryzae. In vitro and in vivo studies under different conditions showed significant response of Mentha piperita, Azadirachta indica and Aloe vera either tested individually or in combination of two or more than two dec...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro
...rane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination, pH was determined by digital pH meter. Motility was determined under microscope at10x magnification, morphology and live dead ratio was assessed using eosin nigrosin stain under 40x magnification,

 *Irum Javed1, Muhammad Asgher2, Sadia Noreen1, Humara Naz Majeed1 and Tanzila Sahar1

SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE
...that Yeast extract in 1% concentration was the best nitrogen source. Surfactants like Tween 80 and sodium dodecyl sulphat (SDS) showed no effect on the citric acid.

...

 Muhammad Akram and Faheem Aftab

THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
...plemented with different concentration (0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1,2,3,4 or 5uM) of TDZ for 24 days. Highest bud break (100%) was obtained at 0.005 uM TDZ after 14 days of initial culture. The same cultures were further maintained and subsequently obtained with 20.6 mm shoot length and 7.2 average number of leaves for another 10 days. Similarly, all other TDZ’s concentrations below 1 uM were also effective i...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
...tent. Minimum inhibitory concentration of heavy metals for all bacterial isolates was different. It was concluded that irrigated pathogenic bacteria are highly resistant to heavy metals.

...

 Tanzeela Riaz*†, Farah Rauf Shakoori, Syed Shahid Ali

Toxicity of phosphine against tolerant and susceptible populations of Trogoderma granarium collected from Punjab, Pakistan
... to different sub lethal concentrations of phosphine for 20 hours. Based on LC50 data, Gujranwala population was found to be most resistant to phosphine whereas Sargodha population was found the least resistant population. Gujranwala population required 113.5, 128.8 and 168.1% more phosphine concentration in 4

 Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4

Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan
...tudy period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment samples was 71.08±8.32 mg/L. Maximum Cr concentration 81.72±2.54 was recorded during February while minimum 61.24±1.23 mg/L during May. Among all the three fish speci...

 Zubair Ahmed#, Muhammad Farhanullah Khan, Habiballah Rana*

Toxicological effects of Haloxylon recurvum Bunge ex Boiss (Khar Boti) whole plant extract and novel insecticide chlorantraniliprole against maize weevil, Sitophilus zeamais Motschulsky
...antraniliprole. A serial concentration of extract i.e.,
1.2%, 2.4%, 3.6%, 4.8%, 6.0% and insecticide 0.00224%, 0.00448%, 0.00896%,
0.01792%, 0.02688%, respectively, were topically applied to adult S. zeamais.
Contact toxicity assayed by topical application showed that 3.6% concentration of
H. recurvum plant extract and 0.00896% concentration of chlorantranilipro...

 Anber Naqvi1*#, Abdul Qadir1, Adeel Mahmood2, Mehvish Mumtaz3, Iqra Aslam1, Gan Zhang4

Assessment of polychlorinated biphenyls (PCBs) in maternal blood serum from selected districts of Punjab, Pakistan
...akistan. The mean
concentration of Σ34 PCB congeners was recorded 232.3 ng/g l.w. PCB homologs
profile showed highest levels of Tetra-CBs (53%) followed by Penta-CBs (23%)
and Hexa-CBs(11%), respectively. Spatial distribution of PCB homolog reflected
the higher prevalence of lower chlorinated PCBs in urban population descending
towards rural population. The mean TEQ concentration of e...

 Siddra Tayyab Akhtar, Anjum Nasim Sabri

Twitching, swimming, swarming in biofilm forming strains in response to chemical and physical factors
...tilities when the
concentration of NaCl increased in medium. Among chemical factors, tobramycin
was proven to have excellent efficiency to reduce bacterial motilites whereas
proteinase K enzyme showed weak antimotility effect against all tested bacterial
strains. From this study we proposed that instead of biofilm detachment by
expensive commercial detergents, we could change the physical environment of
sewage systems ...

Muhammad Asghar1*, Mirza Muhammad Qadeer Baig1, Sanaullalh Chaudhary1 and Muhammad Anjum Ali2 

...noconazole) with various concentrations of sprayable formulations of macronutrients (NPK) was conducted during Kharif 2012 to 14 at Adaptive Research Farm, Gujranwala, Pakistan. The experiment was conducted on a promising basmati rice cultivars Super basmati in RCBD with three repeats. The spray of difenoconazole at rate of 315 mlha-1 + 500gha-1 of NPK (20:20:20) significantly decreased incidence of BLS per leaf (9.31) and resulted in significant increase in 1...
Atiq-ur-Rehman1,2,*, Abida Latif1,*, Rukhsana Anwar1, Nasir Abbas1, Sajid Nawaz2 and Hamid Turab Mirza3
...ibitory assay. Different concentrations of fractions (20-100 µg/ml) were subjected to in vitro α-amylase inhibitory assay. Assessment of anti-hyperglycemic potential of the most active chloroform fraction exhibiting in vitro α-amylase inhibition was carried out in Wistar albino rat models. Changes in postprandial levels of blood glucose in rat models (normal and diabetic) were examined after starch load with or without chlorofor...
Muhammad Muddassir Ali1,2 and İbrahim Hakkı Ciğerci1,*
...mn;1) at 200 µg/ml concentration and 11.67±2.52 at 50 µg/ml. Ethanol extracts showed the 2nd highest DNA damage (19±2)at 200 µg/ml concentration and 11 ±1at 50 µg/ml. While least was observed in the hexane extract (10.33±1.15; 6±2) at both concentrations, respectively. All groups were significantly different (P<0.05) ...

Misbahullah, Shad Khan Khalil, Asim Muhammad*, Afza Tabassum and Shah Fahad  

...rghum extract with three concentrations (1:3, 1:4, 1:5), weedicide (affinity) with three different levels (1/3, 1/ 2, 2/3 of the recommended dose) and 3 times of application (emergence,tillering,50% at emergence + fifty percent at tillering). Herbicide sole, weeded by hand and un weeded plots used for comparison. Results showing that concentration of sorghum and appliance point considerably increased days to heading, anthesi...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
... lead (Pb) and zinc (Zn) concentrations in bat tissues through atomic absorption spectrophotometer. Our findings showed that metals were more concentrated in the liver and kidneys, Zn and Pb showed high mean values as compared to other metals but these values were below the lethal levels. Within the regions and sexes no significant variations were found in metal concentrations; however within three bat species metal
Muhammad Ijaz1, Shahid Hussain Farooqi1, Rahmatullah2, Amjad Islam Aqib1, Sadaqat Ali3,*, Awais Ghaffar1, Ahmad Ali1 and Sehrish Saleem1
...copper, iron and lithium concentrations significantly (p < 0.05) decreased while the potassium concentration significantly (p < 0.05) increased in diarrheic foals. Hence, this can be concluded that diarrhea in foals have significant correlation with PCV, serum electrolytes, and trace and ultra-trace elements.
...
Mirza Imran Shahzad1, Hina Ashraf2,*, Muhammad Arshad3, Sabeeha Parveen4, Amna Aslam4, Nargis Naz4, Zahid Kamran1, Sumbul Gohar Khalid5, Sajid Hameed1, Muhammad Ashfaq6 and Muhammad Mukhtar7

 

...urther used in different concentrations and IC50 of each extract was calculated. The extract of Achyranthes aspera was found most effective with IC50 3.125μg and the extract of Oxystelma esculentum, was found least effective with IC50 60μg in the series. The current study has established the fact that Cholistani plants are rich source of anti-NDV agents and can be used in purified or in crude form.
...
Fatih Korkmaz1, Veysel Parlak2, Özgür Kaynar3, Arzu Ucar2, Gonca Alak1,* and Muhammed Atamanalp2
...lts in increased protein concentration compared to other coatings and control group (p<0.05).
...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...growth hormone and IGF-1 concentrations and average body weight than those in the control group (P < 0.05). Immunisation with ptCS/2SS-asd promoted the growth of rams similar to the effect of the simple co-injection of CpG and SS DNA vaccine. Thus, the fusion type of CpG adjuvant is an effective, simple, and low-cost method in enhancing the effect of SS DNA vaccine and the growth of ram lambs.
...

Nighat Seema1*, Muhammad Hamayun1, Husan Ara1 and Raham Sheer Khan

...ght stress, ammonium ion concentration was increased in all endophytic treated soil except that of A. niger. Similarly, inoculation of endophytic fungi did also increased the value of soil nitrate ions in the range of 26.86±1.34~39.39±1.97 mg kg-1 with or without drought stress. Inoculation of endophytic fungi increased the amount of extractable P and K in growth media compared to soil samples before experiment and after experiment in control sam...
Asim Faraz1,*, Muhammad Younas2, Abdul Waheed1, Muhammad Yaqoob2 and Kashif Ishaq3

 

...a, Mg, Cu, Zn, Fe and Mn concentrations were found to be significantly different (P<0.05) between calf groups being higher in IMS as (685.07±15.86, 595.67±15.86; 104.33±5.12, 101.17±5.12; 7.08±0.34, 6.73±0.34; 65.33±2.25, 59.33±2.25; 322.20±8.67, 311.10±8.67; 46.53±1.83, 40.67±1.83 mg/dL in male and female) than SIMS (523.18±15.86, 486.98±15.86; 80.58±5....
Aqeel Mehboob, Syed Muhammad Zaka*, Muhammad Sarmadand Maryam Bajwa
...iculata at 125 mg/ml concentration was maximum (83.64±12.51), followed by P. nigrum and M. oleifera at 125 mg/ml concentration were 79.76±14.60 and 30.57±4.80, respectively. The higher oviposition deterrent index was recorded in M. paniculata extract (87.32±0.77) as compared to P. nigrum and M. oleifera extract i.e. 82.58±2.14 and 77.25±1...
Muhammad Huzaifa Mehmood1, Muhammad Ahmad Iqbal2, Muhammad Daood1, Muhammad Rizwan Tariq3*, Khubaib Ali3
...d lipid profile and PUFA concentration was significantly affected by the incorporation of oat in rabbit feed. It was concluded that the supplementation of 2% oat in rabbit feed increase n-3 PUFA in meat while improvement in serum lipid profile was observed by 4% supplementation of oat seeds. It was also observed that fat percentage in meat was also reduced by oat seed supplementation.
...
Rahat Afza, Muhammad Afzal, Muhammad Zeeshan Majeed and Muhammad Asam Riaz*
... the impact of sublethal concentrations of insecticides on intra-guild predation (IGP) and aphid consumption by coccinellids. Data revealed that in the absence of prey larvae of C. septempunctata exhibited more predation on C. transversalis while the predation of C. septempunctata on C. transversalis was relatively less in the presence of prey. The larvae of C. septempunctata were found stronger and competitive than larvae an...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
...ncorporation method. Two concentrations of a NPV based formulation (Somestar): 1x107 and 1x108 POB/ml, and three concentrations of a Bt based formulation (Dipel): viz., 0.1, 0.3 and 0.5 μg/g, were applied alone and in different combinations against 2nd and 4th instar larvae of S. litura. The highest mortality of larvae was recorded after 7 days of applicatio...
Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1
...e of Hemodialysis on the concentration of the CXCL8 in serum and the levels of CXCL8 mRNA in neutrophils were respectively measured by ELISA and PCR. The house keeping gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference, the ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in neutrophils of the chronic renal failure were (128.892±17.101) pg...
Peng Song1,2, Wei Feng2, Haiying Shi2,Jinsheng Zhao3, Renmin Liu3 and Wei Xu2,*
...e maximum decolorization concentration of the Amaranth reached 1450mg/L. Mg2+ and Fe3+ could accelerate Amaranth decolorization, and Cu2+, Zn2+, Co2+ showed inhibition of Amaranth decolorization. Compared with the traditional method dissolving oil-soluble dye in DMSO, HP-β-CD was used to monitor the decolorization rate of the sudan I and appropriate molar ratio of HP-β-CD to dye (nC:n<...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
...the minimal bactericidal concentration, change of pH in the first 12 h and OD values of biofilms. Besides, fluorescent images of exopolysaccharides and living bacteria of Streptococcus mutans were measured with confocal laser scanning microscopy (CLSM). In our study, capsaicin showed an outstanding performance in the inhibition of acid producing and biofilm of the four strains of cariogenic bacteria. Meanwhile, low values of MIC of capsaicin on four sec...

 Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2

...e treated with different concentrations i.e., 2, 4, 6 and 8 mg/ml of ethanolic extract and motility of the worms was examined after every two to eight hours. Both A. lebbeck and C. sinensis exhibited 88% and 95% mortality at 6 & 8 mg/ml after 8 hrs of treatment. In conclusion, the ethanolic extracts of A. lebbeck L. and C. sinensis L. showed in vitro anthelmintic activity against H. contortus at

Ali Awais, Charassri Nualsri and Watcharin Soonsuwon* 

...treated with varying EMS concentrations. With the increase in concentration, there was a continuous decrease in germination, shoot and root lengths respectively. Data have been subjected to regression analysis and effective doses of EMS were recorded for both genotypes. The effective value calculated for shoot and root length in Dawk Pa-yawm was 1.34% and 1.33% and in Dawk Kha 50, 1.23% and 1.86% respectively based on the re...

Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1

...through ELISA. Different concentrations of SA and BTH were exogenously applied to activate the inherent resistance of mungbean by the production of defense associated compounds. All treatments were supportive in suppressing plant infection as compared to infected control, but the most promising outcome of treatments was observed when SA and BTH were applied at the concentration of 5mM and 150mg/L respectively. Three weeks&rs...
Fariha Latif* and Muhammad Javed
...50 and lethal concentration. These acute toxicity tests were performed at constant temperature (30oC), pH (8) and total hardness (250 mgL-1). All the three fish species varied significantly in their sensitivity towards metals mixture with the C. catla beingsignificantly highly sensitive followed by L. rohita and C. mrigala. After the acute toxicity tests, fish were exposed to 2/3rd of their respe...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
...ancorpuscular hemoglobin concentration) quantity within both animal categories.
...
Hafiz Abdullah Akbar, Zafar Iqbal, Waqas Raza*, MuhammadUsman Ghazanfar and Salman Ahmad
...mbination with wax and 3 concentrations of salicylic acid alone and in combination with wax. The study revealed that in case of plant extracts maximum growth inhibition (35.90 %) was shown by Anar followed by Chabir (33.60%) and Garlic (29.16%), while minimum growth inhibition was recorded from Parthanium (17.43%) and Ginger (19.94 %). In case of plant extracts + wax maximum growth inhibition (44.36%) was shown by Anar+Wax followed by Chabir+Wax (42.78%) and F...
Song Jiang, Fa-Lin Zhou, Jian-Hua Huang, Qi-Bin Yang* and Li-Shi Yang
...ery 5 d. Bacillus concentrations in the Y1, Y2 and Y3 groups were 10 ppm, 15 ppm and 20 ppm, respectively, and the Lactobacillus concentrations in the R1, R2 and R3 groups were 20 ppm, 25 ppm and 30 ppm. The control (C) group had no microecological preparation added. Results showed that the Y2 larvae achieved the heaviest body weights, which were significantly higher than those of all other groups (P <...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...was recorded the optimal concentration for the maximal cellulase activity. SDS-PAGE analysis confirmed the molecular weight of recombinant protein as 39 kDa. The protein was found thermostable which retained more than 70% residual activity with an incubation of 1.67h at 90 °C in the presence of cobalt. Presence of ionic and non-ionic detergents showed an inhibitory effect on the enzyme activity. Kinetic studies of recombinant protein demonstrated the k<...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
...e susceptible to certain concentrations of Huwa-San TR50: 4,000, 8,000, 10,000, 12,000 and 14,000 ppm. A marked reduction in the movement of both tick species was observed 24h of applying Huwa-San TR50, at 10,000 ppm and above. This study recorded an increase in percentage mortality with increasing concentrations of Huwa-San TR50 and this resulted in mortalities of 66.5% for H. dromedarii and 76.5% for H. impeltatu...
Tahira Moeen Khan* and Amat ul Mateen
...s a linear curve between concentration and absorbance. The uv-vis spectrum revealed the shift of absorption towards longer wavelength with the increasing concentration of plant extract. XRD indicates CuO Nps particle size is in between 14-20 nm. CuO nanoparticle show very significant antibacterial activity against four bacterial strains three gram negative (Escherichia coli, Proteus mirabilis, Salmonella) and one gram...

 Syed Turab Raza1,*, Zhu Bo1,*, Zulfiqar Ali2 and Tang Jia Liang3

... cow manure in different concentrations. Vermicomposting through biochar (Biochar 50%, rapeseed residues 20% and Biochar with earthworms) gave maximum increase in soil fertility with maximum decrease (39.63%) in C:N ratio. Significant increase of N content in nutrients and reduction in C: N ratios during this process shows conversion of harmful wastes into useful fertilizer.

...
Shehzad Ghayyur1,3, Sadia Tabassum1, Munawar Saleem Ahmad2Naveed Akhtar1 and Muhammad Fiaz Khan1,*
...

 Effect of three concentrations (0.25, 0.75 and 1.25ppb) of chlorpyrifos administered for 96 h has been studied on hematological and seral biochemical components of Oreochromis mossambicus. RBCs, Hb and Hct decrease significantly (p < 0.05) indicating an anemic condition, while the WBCs and platelet counts increased significantly (p < 0.05) in all the treated groups compared to the control group. Among the seral biochemical parameters, the level ...

Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
... larvae. However, higher concentrations of A. indica oil can be used to control CLM larvae.

...

 Abdul Khaliq1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Akhlaq Ahmad2 and Yasir Iftikhar3

...esponse was noted at 10% concentrations of all plants but E. Camaldulensis (5%) was least effective against all insects after control. Resurgence response in F1 generation in each experiment showed high multiplication rate at low concentrations and vice-versa also suppressed against N. tabacum and C. procera. Hence, developing PH3 resistance can be managed with bio-pesticides up to ...

 Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2 and Muhammad Anjum Zia3

...i> exposed to sub-lethal concentration for 14 days was evaluated. After purification, specific activity of enzyme was 1750.13 and 1272.72 U mg-1 in kidney of metal exposed and control fish, respectively. The enzyme from metal exposed kidney was 2.68 fold purified with 58.73 % age recovery. Results of characterization showed that SOD had broad range of pH from 4 to 8.5 but show maximum activity at pH 7 and 7.5 for stressed and control fish, respectiv...

 Şaban Çelebi

... their combination on Se concentration in liver, heart, kidney and breast muscle. In addition, malondialdehyde (MDA) concentration and enzyme activities of glutathioneperoxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) in liver, heart, breast and thigh tissues of laying hens were determined in present study. A total of 96 White Lohman laying hens, aged 24 weeks, were randomly divided into 4 groups (n=24), each...
Fazal Abbas1, Muhammad Ijaz1,*, Zunaira Akhtar1, Khalid Mehmood2, Muhammad Zeeshan Hyder3 and Umair Iqbal4
...The sodium and potassium concentration in ppm significantly increased (P< 0.001) while the calcium, copper and iron concentration in ppm significantly decreased (P< 0.001) in the calves having diarrhea. Copper concentration is related to the packed cell volume as in anemia copper deficiency is a significant feature. It was concluded that neonatal calf diarrhea is much common in calve...
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
...ector. Effect of various concentration of Isopropyl β-D-1-thiogalactopyranoside (IPTG), post induction time, effect of temperature and pH were examined for the maximal production of recombinant cellulase. The effect of supplementation of LB medium with additional carbon and nitrogen sources was also analyzed for maximal production of recombinant protein. Higher level enzyme activity was recorded at 25°C, pH 7.0 when the cells were induced with 0.5 m
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...pectively when used at a concentration of 1mM, however, the enzyme was almost completely inhibited by Pb+2 and Hg+2. Purified polygalacturonase showed pectinolytic effect in juice clarification by attaining maximum clarity (95% transmittance) after incubation at 50°C for 60 min. Total amount of phenolic and free radical scavenging activity of juice was improved after clarification. To understand the effect of juice clarification param...
Bushra Siddique1,*, Muhammad Tariq1, Muhammad Naeem1 and Muhammad Ali2
...ilter paper strips at 3% concentration. The filter paper strips were placed in arms of olfactometer. The control arms were treated with n-hexane. Data pertaining to preference of C. septempunctata and D. rapae after treatment application were recorded and analysed statistically. It was found that T6 (β-pinene + E-β-Farnesene) exhibited highest mean number entries of C. septempunctata (6.13%) and highest m...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
... the rising amount of TA concentration. In our study, in 25, 50, and 100 μM TA groups, cyclooxygenase-2 (COX-2) decreased incrementally compared to the control group. Considering overstimulation of COX-2 paths in emergence of cancer or in metastasis, this study shows TA has an antiproliferative and stimulating effects on apoptosis of LNCaP cells based on the dose and duration of exposure.
...

Hamid Ali, Muhammad Arshad, Ibad Ullah Jan, Muhammad Zamin*, Junaid Khan, Ikram Ullah and Mushtaq Ali 

...f tuberose under various concentrations of gibberellic acid and micronutrients. Experiment was laid in Randomized Complete Block Design (RCBD) using two factors i.e. gibberellic acid and micronutrients with three replications. Both the factors gibberellic acid (GA3) and micro nutrients had their significant effects. The best performance was found in the interaction of GA3 and micronutrients for plant height (53,21cm), number of leaves (16.34), length of spike ...

Muhammad Usman Ghani1*, Muti Ur Rehaman Khan1, Asim Aslam1, Zubair Shabbir2, Li Bo3 and Naveed Anwar4 

..., platelets count and Hb concentration in diseased animals representing hematological investigation.  

...
Sun Hong1, Zhang Yifeng1, Wang Baishi2, Li Yangwei1, Xu Wenbo1, Mao Runkun1 and Wang Zhenglong1,*
...dioxide (CO2) concentrations and the temperature and humidity in the underground burrows of mandarin voles in Xinzheng, Henan Province, China during the spring and summer of 2015. Our results show that 1) the depth and complexity of the burrow were higher during summer than during spring; 2) maximal CO2 levels (2.55%) and minimal O2 levels (16.04%) were recorded in the underground burrows during summer; and 3) the temperature a...

Aliya Ayaz, Gohar Ayub, Maqsood Khan* and Syed Aizaz Ali Shah 

...r A was gibberellic acid concentrations i.e. 0, 20, 40 and 60 mgL-1, while factor B was comprised of dipping durations i.e. 0, 6, 12, and 18 hours respectively. Gibberellic acid concentrations and dipping durations had significant effect on the growth and yield of bitter gourd. Gibberellic acid concentration at @ 60 mgL-1 significantly reduced days to emergence and days to first flower, wh...

Zahin Anjum1*, Farhat Shehzad1, Amina Rahat1, Hamid Ullah Shah2 and Saleem Khan3 

...d Dibenz(a,h) anthracene concentrations were also found high in fish and mutton. The observed values were greater than permissible limits determined by Codex Alimentaurius and World Health Organization. High concentration of Polyaromatic Hydrocarbons was observed in coal grilled meats. Anthracene content was found high in mutton meat and mean value was 12.50 µg/kg-1; flouernthene was assessed high in fish meat with mea...
Iqra Jaffar, Zainab Sehzadi, Muhammad Adeel, Khalid P. Lone and Mehwish Faheem*
...iles were exposed to low concentrations (10μg/l and 100μg/l) of di-n-butyl phthalate (DBP) for 7 days to evaluate oxidative stress in liver and gills by analyzing activity of glutathione-S-transferase (GST), catalase (CAT), glutathione (GSH) and by measuring lipid peroxidation (LPO). Catalase activity increased significantly in liver and gills of fish exposed to 10μg/l of DBP. Level of GSH and LPO increased significantly in gills while a non-significa...
Abid Hussain Shahzad1, Abdul Sattar1,*, Nasim Ahmad1, Ijaz Ahmad2, Deniz Nak3 and Yavuz Nak3
...7), respectively. The P4 concentration has significant (P<0.05) difference among OVP0 (6.52±0.32), OVP5 (7.75±0.38) and OVP7 (7.58±0.26) on d30 post AI. This difference was non-significant (P > 0.05) on d60 post AI in OVP0 (6.37±0.49), OVP5 (6.75±0.36) and OVP7 (6.80±0.41), respectively. On d30 post AI, pregnancy rate was 39.70, 42.60 and 45.50% in OVP0, OVP5 and OVP7 groups, respectively ...
Mohsan Iqbal1, Farid Asif Shaheen1, Rashid Mahmood2,*, Muhammad Khalid Rafique2, Imran Bodlah1, Farah Naz3 and Muhammad Usman Raja3
... observed in synergistic concentration of EPB as compared toEPF. Synergistic concentration (1×108) of bacteria gave the best results against C. chinensis as compared to that of entomopathogenic fungi. The maximum percent conidial germination (90%) of B. bassiana and percent mortality (50.40±2.20a), (79.70±0.28a) and (90.76±0.56a) of C. chinensis were observed ...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
... in Friesian bull. Sperm concentration and motility were significantly (P<0.05) high in Jersey bull while live sperm percentage was significantly (P<0.05) high in Jersey bulls and sperm abnormality was significantly (P<0.05) high in Sahiwal and Friesian-Sahiwal cross. In addition, semen quality was improved in all the breeds after supplementation of vitamin C. There was no significant effect of BCS on the sperm quality of bulls. The results give impor...

Imdad Ali Mahmood1, Muhammad Imran2, Muhammad Sarwar1, Matiullah Khan1, Muhammad Aqeel Sarwar2, Shoaib Ahmed1 and Shahid Riaz Malik

...maturity to determine Zn concentration in plant tissues and grains. A significant increase in pods per peduncle, seeds per pod, grain yield and total biomass of lentil was observed with Zn application even at lower rate (5 kg ha-1) under PGPR inoculation. Maximum 100-seed weight (2.56 g) was recorded with PGPR inoculation which was 21 % higher than that of without PGPR inoculation. Overall, a significant increase (31 %) in grain yield and total biomass was obs...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...ificantly improved under concentrations of Vitamin C (400 mg/L) and Vitamin E (600 mg/L) (P < 0.05). Moreover, physiological indices of donkey semen were significantly higher than the control groups (P < 0.05) when Vitamin C (200 mg/L) and Vitamin E (200 mg/L) were present simultaneitily in the medium. Overall, the results suggested both antioxidants (Vitamin C and Vitamin E) could increase frozen semen quality and the ability of antioxidan...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
...was treated with various concentrations (5, 40, 80 and 100µM) of salicylidene acylhydrazide and 5-15 µM of proteases (proteinase K, trypsin, and chymotrypsin). Concentration dependent decrease was observed after 72 hours in biofilm formation of D. vulgaris on treatment with salicylidene acylhydrazide. Whereas 15 µM concentration of proteases produced significantly ...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
... showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48 h) was more active, being able to kill 100% of the Acanthamoeba cysts.The results showed the cysticidal activity of T. ammi essential oil, LC50 values estimated to be 73.31, 61.69, 57.21 and 53.01 μg/mL with 24, 48, 72 and 96 h, respectively. This is the first report of in vitro cysticidal activity of T. ammi essential oi...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...d along with the extract concentration and exposure time. Botanical extracts of Azadirachta indica (neem) and Gardenia jasminoides (gardenia) were the most effective with minimum LC50 (20.00 and 42.19%, respectively) and LT50 (47.97 and 71.26 h, respectively) values followed by Nerium indicum (oleander). Moreover, the essential oils of Datura alba (dhatura) and Syzygium aromaticum (clove bud) were the mo...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...xposed to the sub-lethal concentration (1/3rd of LC50) for 14 days. The CAT was partially purified by using ammonium sulphate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT remain lowe...

Sadaf Rahim and Mudassar Iqbal* 

... respectively at highest concentration after 2 hours exposure. Increased potency of extracts was observed with the increased exposure time. The LC50 value calculated for organic extract obtained from T. harzianum grown on PDB against Diuraphis noxia was 398.69 μg mL-1 after 3 hours with increased potency (237.46 μg mL-1) after 24 hours, whereas against Tribolium castaneum it was 213.85 μgmL-1 after 24 hours while its decreased potential was recorded (...

Muhammad Shafique, Nosheen Noor Elahi*, Muhammad Rashid, Amjad Farooq and Kausar Hussain Shah

...reased at 100-300mM NaCI concentration. Inoculation effectively increased the dry weights of plants at salinity levels of 0-50 mM NaCI as compared to uninoculated plants but was not effective at 200-300mM NaCI. Number and fresh weight of pods were not affected at 0-100 mM NaCI levels of salinity but strongly decreased at 200-300 mM NaCl. Number and fresh weights of nodules increased slightly at lower levels (20- I00 mM) of salinity but strongly decreased at 20...

Munazza Yousra1*, Sair Sarwar1, Muhammad Mahmood Ul Hassan1, Muhammad Zameer khan1, Abdul khaliq2, Shahbaz Ahmad1 and Shamim Ul Sibtain Shah3 

...n sources showed that Zn concentration was maximum on the first day in all treatments except control. Zinc release from Zn-EDTA was much higher as compared to ZnSO4. Similarly, the pot experiment conducted with the same set of treatments and crop responded well to Zn fertilizer application. The results showed a consistent increase in Zn concentration with the increasing Zn doses in both varieties. The maximum Zn

Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Gul Bahar Khaskheli2, Allah Jurio Khaskheli3, Arshad Ali Khaskheli4, Abdul Samad Magsi5, Ghulam Shabir Barham2, Arab Khan Lund5 and Maqbool Ahmed Jamali4 

...essed significantly high concentration of crude protein. Further the Prosopis juliflora against Zea mays, in Acacia nilotica versus Alhagi maurorum, in Tamarix orientalis versus Trifolium alexandrinum, Tamarix gallica versus Cordia sinensis (Linn.) crude protein content existed statistically non-significant, but each of above set varied significantly to one another. Trifolium alexandrinum and Zea mays though had statistically similar c...
Khansa Nazir1,*, Tariq Mukhtar1 and Humayun Javed2
...eniles was recorded at a concentration of 100 mg/ml followed by 75 mg/ml of AgNP. The minimum mortality was recorded with 25 mg/ml of AgNP. With the increase in concentration, there was a corresponding increase in the mortality of juveniles showing a direct relationship between mortality and concentration of nanoparticles. The effect of time on mortality was also found significant. With th...
Riyadh S. Aljumaah1, Mutassim M. Abdelrahman1,*, Moez Ayadi1 and Abdullah H. Alyemni2
...he groups, however, urea concentration was significantly (P<0.05) in the T1 and T2 groups. In conclusion, feeding Najdi ewes a diet containing higher energy and protein than recommended by National Research Council (1985), in comparison with the traditional feeding system, improved the lambing rate and growth of the newborns.
...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...s of the frozen mass and concentration of enzymes was determined. It was found that more than ~85% mass of enzymes, migrated along with freezing front and concentrated in the centre in case of radial freezing. To examine the effect of unidirectional freezing over extracted enzymes, the working activity of separated enzymes was checked by performing desizing test for alpha-amylase and biopolishing test for cellulase. The noteworthy results of desizing and biopo...
Naimat Ullah1, Sarzamin Khan2, Muhammad Mobashar3, Shahbaz Javaid4*, Tarique Tonio1 and Asif Mahmood5
...perm motility (%), sperm concentration (million/ml), live to dead ratio of sperm, amplitude of lateral head displacement of sperm (µm) and straightness of sperm (%) were measured at interval of 15 days. The results showed that dietary supplementation of fat didn’t influence dry matter and water intakes as well as weight gain significantly (P>0.05) among different treatments. Body conditions score was recorded significantly higher (P<0.05) in ...
Ali Mujtab Shah1,2,3, Muhammad Naeem2, Muhammad Giasuddin Shah2, Muhammad Haaroon2, Quanhui Peng1 and Zhisheng Wang1,*
...rom the present study theconcentration of serum IgG following administration of colostrum by ST method increases at a rate to that in calves fed by NS and NB and reaches a level consistent with high calf survival, it is also concluded from the study that the body weight gain is increased by ST feeding. 
...

Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3

...luding different glucose concentrations, nitrogen sources, pH, temperature and incubation period influence on biomass and different enzymes viz; xylanase, CMCase and laccase production by Pleurotus ostreatus were optimized in shake flask cultures. The results indicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (...
Xiaopeng Tang1, Wen-qin Su1 and Re-jun Fang1,2,*
...orphology and phosphorus concentration in the cell supernatant were measured after 24 h of CT treatment. The NaPi-IIb protein expression was determined by Western Blot, and the NaPi-IIb mRNA expression was determined by RT-PCR. The results showed that, compared with the control group, different levels of CT had no effect on cell proliferation, but it inhibited (P < 0.05) the absorption of phosphorus at CT concentration...
Constance Obiageli Ejilibe1, Helen O. Nwamba2, Ifeanyi Chinedu Atama3,*, Chiamaka Lynda Ani2, Ifeanyi Oscar Aguzie3, Josephine C. Madu3 and Christopher Didigwu Nwani3
...) and total protein (TP) concentrations in homogenized muscle samples was determined by standard procedures. ALT, AST and ALP increased significantly in response to concentrations of Butaforce® (7.00, 9.00 and 11.00 µgL-1) and Termex® (15.00, 20.00 and 25.00 µgL-1) used. The TP decreased in response to the same concentrations ...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5 
...upplemented with various concentrations of L-cysteine 0 mM, 0.5 mM, 1.0 mM and 1.5 mM. Cooling and equilibration of the diluted semen was done at 4°C for 4 h subsequently filled in straws at the same temperature and were cooled in liquid nitrogen vapors for 10 min. followed by storage at -196°C for assessment. Progressive motility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-cysteine supplemented groups in comparison wi...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
... mg/kg), decreased sperm concentration (for 110 mg/kg dosage), decreased motility (for 110 mg/kg dosage) and increased sperm malformation (for all of the treated groups) were also confirmed (p<0.05). Sperm malformation showed high sensitivity to PFOS exposure. A sperm malformation percentage, varying from 11.00% to 51.20%, was confirmed for the treated groups. As to various sperm malformation types, curved body type constituted the most proportion. T...

Chukwuemeka Calistus Okolo1*, Ikenna Onyema Ezeh2, Chinelo Nnenna Uju3 and Nwakaego Ernestina Nweze1 

...te count and haemoglobin concentration compared to groups A--C, and E, no significant variation was seen in the total and differential leucocyte counts across all infected groups. By day 23 PS, groups A and B had similar serum alanine and aspartate aminotransferases, blood urea nitrogen, and serum creatinine levels as group D. The serum malondialdehyde and catalase levels were similar in groups A--D. Therefore, treatment with the probiotics, while enhancing cl...
Adel E.M. Mahmoud1, AdamCieślak2, Paulina Szulc2, Anna Łukomska3 and Małgorzata Szumacher-Strabel2,*
...d in higher (P<0.001) concentration of Holotrichs. The ratio of acetate to propionate increased linearly with inclusion of dried sugar beet in the diet, however statistically significant differences were found only in D4. On basis of presented data, it could be concluded that yellow corn could be partially of fully substituted by dried sugar beet pulp without negative impact on rumen parameters including dry matter digestibility.
...
Tanzeela Riaz1, Farah Rauf Shakoori2,*, Hareem Mansoor1, Sana Khan1 and Mushtaq A. Saleem1
...to figure out the lethal concentration (LC50) of emamectin, abamectin and spinosad alone and in various conbinations such as abamectin:emamectin (1:1, 2:1, 3:1, 1:2 and 1:3) and abamectin:spinosad (1:1, 2:1, 3:1, 1:2 and 1:3) against two larval instars (4th and 6th) of MBDIN and Lahore populations of T. granarium. The LC50 of abamectin, emamectin and spinosad for 4th larval instars of Lahore populat...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
...he effects of sub-lethal concentrations of manganese on antioxidant activity in the fish, Labeo rohita. Fish were acclimatized for two weeks in the cemented tanks prior to the start of experiment. Four groups (n=10) of one year old fingerlings of Labeo rohita were exposed to 96-hr LC50 and sub-lethal concentrations (2/3rd,1/4th and 1/5th of LC50) of mangane...

Matiullah Khan*, Shameemul Sibtain Shah, Atiqullah Khan, Muhammad Sarwar and Shoaib Ahmed 

...se in postharvest soil P concentration was recorded. Seed inoculation with PSB significantly increased grain and dry matter yield. It can be concluded from the study that good quality of SSP can be manually prepared from HRP which may increase the yield comparable to DAP. 

...

Masood Ahmad* and Abdur Rab 

...eraction between calcium concentrations and application time revealed that foliar application of 200 mM calcium at 5th leaf stage resulted in significant increase in leaf area, however application of the same concentration of calcium at 7th leaf stage resulted in maximum size and weight of daughter corm. It is concluded that foliar application of 200 mM calcium at 7th leaf stage increased the size and weight of daughter corm...

Muhammad Zeeshan Manzoor1*, Ghulam Sarwar1, Mukkram Ali Tahir1, Noor-Us-Sabah1, Ayesha Zafar1 and Sher Muhammad2 

...water increased the salt concentration of soil. Resultantly, the growth of plants and yield becomes at threat. The salt stress is translated into decrease in growth rate and resultantly, biomass production decreases significantly. This reduction is gradual, depending upon increase in salt concentration in the root medium, while each species has a critical threshold value where it was started showing negative response. Leachi...
Muhammad Suleman1,*, Abu ul Hassan Faiz2, Muhammad Shahbaz2 and Ayesha Riaz3
...s niger at different concentrations of wheat bran as carbon source (2.5, 3.0, and 3.5 %) on the production of xylanase. Submerged fermentation was carried out in 500 ml Erlenmeyer flasks using vogel’s medium at 35°C for five days. UV irradiated A. niger has significantly increased the xylanase production as compared to parent strains. Twenty minutes exposure of A. niger was considered suitable for the production of xylanase. Wheat b...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...d growth. Changes in GHR concentration affect signalling pathways, binding capacity and alters activity of GH. Therefore, the GH and GHR genes are considered important candidate genes. DNA samples of Marecha camel were collected from the Camel Breeding and Research Station at Rakhmani Bhakar, Pakistan. Two significant polymorphic sites were identified in GH gene and three in GHR gene. Out of these, T1720A polymorphism in GH and A211927G polymorphism in GHR gen...
Abdul Hanan1, Talha Nazir1, Abdul Basit1, Shahbaz Ahmad2 and Dewen Qiu1,*
...ted with three different concentrations (1×106, 1×107 and 1×108 conidia/ml), filtrate bioassay was estimated by applying 7th day fungal filtrate and binary combination (1ml conidia +1ml filtrate) of V2×V2, V3×V3 and V5×V5 were tested against M. persicae. Mortality data were recorded after 2, 4, 6 and 8 days post-treatment. In conidial bioassay, V-3 showed maximum aphid morta...
Muhammad Zameer Khan1, Sair Sarwar1*, Ahmad Khan1, Razaullah Khan1, Munazza Yousra1 and Muhammad Ilyas2
...ield components, N, P, K concentration and uptake by wheat. Two extractants namely, AB-DTPAand NH4OAc were tested for K extraction from soils. The results showed a high positive correlation between AB-DTPA and NH4OAc extractable K, plant growth and yield parameters. The amounts of soil K had positive correlation with shoot K concentration and grain yield of wheat with r = 0.48 and 0.49, respectively for...
Aqsa Pervaiz1*, Rukhshanda Afridi1, Zahra Pervaiz1, Rabia Masood1 and Hira Pervaiz2
...ere exposed to sublethal concentrations of methylmercury 0.044 mg/L, 0.055 mg/ L and 0.073 mg/L in tap water respectively. The fish exhibited tail and fin disruption, bulging of eyes, scales erosion, mucous secretion, hemorrhage, color change, slow down swimming, lake of balance, restlessness, swimming side-wise, retardation of opercular movement and quick sudden movements. The fish was anesthetized and liver were removed after 48 and 96 hours for histopatholo...
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
...ibited the growth at 1ml concentration, Local honey at 3ml concentration, Marhaba’s honey at 4ml concentration, and Young’s honey inhibited the growth at the concentration of 5ml.
...
Naveed Ahmed1*, Abdul Waheed1, Farrukh Siyar Hamid1, Imtiaz Ahmed1, Muhammad Abbas Khan1, Sohail Aslam1, Muhammad Adil Younis2, Seemab Ali1, Nadia Khan1 and Madiha Bashir1
... (Asha and Red) and four concentrations of Gibberellic acid (GA3) (0, 20, 40 and 60 ppm). Experiment was laid out on Randomized Complete Block Design (RCBD) with three replications. Data showed a significant difference among most of the growth parameters. Maximum head weight (2306.7 g), stem length (42.66 cm), number of leaves plant-1(15.73cm), root length (27.53 cm), yield (55.63 t/ha) was recorded for cultivar cabbage red with the appli...
Muhammad Aslam Farooqi*, Bakhtawar Irsa, Sajjad Ali, Asif Sajjad, Muhammad Waqar Hassan and Sohail Akhtar

 

...;5% R.H). Four different concentrations (i.e. 50 ppm, 100, 200 and 400 ppm) of each insecticide were evaluated against A. mellifera as derived from their recommended field doses. Mortality of A. mellifera was recorded at 0.5, 3, 6 and 24 hours after exposure to insecticides. To evaluate the toxic impact, median lethal concentration (LC50) of each tested insecticide was determined. The results ...
Muhammad Usman Akhtar1,2, Abdul Qayum3, Anshan Shan1,*, Shuli Chou1, Hyeonsoo Jo4, Syed Waqas Ali Shah4 and Ishfaq Muhammad5
...of different dietary RDP concentrations on nutrient intake, nutrient digestibility, nitrogen balance, blood urea nitrogen (BUN) and milk yield of lactating Beetal goats. Thirty five lactating Beetal goats were randomly divided into five groups (seven goats per group) in a randomized complete block design. Five isocaloric and isonitrogenous diets were formulated to contain 30, 40, 50, 60 and 70% RDP of dietary protein represented as 30RDP, 40RDP, 50RDP, 60RDP a...
Shad Mahfuz1,2, Shuyuan Wang1, Mo Chen1, Fei Zao1, Dong Zhen1
Zhongjun Liu4 and Hui Song1,3*
...tibiotic fed groups. The concentrations of serum immunoglobulin M (IgM) were slightly higher in FVW fed groups compared with the other groups. Serum albumen concentration was increased (p<0.05) in 4%FVW fed group than control and antibiotics fed groups. Our study demonstrated that dietary inclusion of Flammulina velutipes mushroomstem waste could be used at 4%level as a unique feed supplement to improve egg ...

Muhammad Abu Bakar1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1, Rashid Mahmood2, Ziyad Abdul Qadir2, Muhammad Arshad1 and Mubasshir Sohail3 

...d Neem oil) in different concentrations against percent mortality of Varroa mites. The essential oils were applied at different concentrations (100 ppm, 200 ppm and 400 ppm) and percent mortality data were recorded after 12, 24, 48 and 72 hrs of the application. The results of present study were showed that lemon oil and eucalyptus oils were best compounds to manage Varroa mites at 200 ppm concentrat...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*

 

... and malonaldehyde (MDA) concentration were detected by a total superoxide dismutase test kit and a malondialdehyde test kit. Genomic DNA and RNA were extracted from cells; Methylation was inhibited by treating HL7702 cells with 5-aza-2’-deoxycytidine (AZA), and the level was analyzed via the methylation-specific PCR (MSP) methods. The increases in the methylation rate of CpG islands in the CYP1A1 promoter region and expression of DNA methyltransferases ...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...e further clarified. The concentration of the CXCL8 in serum and the level of CXCL8 mRNA in PBMCs of forty-eight children were dynamically measured by ELISA and PCR. The ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in PBMCs in MPP children were (298.917±51.860) pg/mL and (1.848±0.525) lgcDNA/lgGAPDH. There were significant differences between the trial groups and no...
Muhammad Izhar Shafi* and Muhammad Sharif
...s the lowest available P concentration of 8.09 and 7.82 mg kg-1 was recorded in soils where SSP and NP were applied, respectively, without any amendment of HA. Weekly turnover and mineralization potential increased with incubation periods as well as with the application of different HA levels. Maximum weekly P turnover rate and mineralization potential were 1.35 mg kg-1 and 67.71 kg P ha-1 season-1, were recorded in ...

Asma Hassan*, Zuhair Hasnain, Shahzada Sohail Ijaz, Muhmmad Ansar, Muhmmad Rasheed, Ajaz Ahmad, Lubna Ayub Durani and Muhmmad Bilal Ali 

...the highest nitrogen (N) concentration was under MT with GW (5.5 mg kg-1), 2011-12 it was more in GW (6.13 mg kg-1) under MP. In winter from second year it was highest in MP with MC (8.4 mg kg-1). Phosphorus (P) and potassium (K) concentration second year it was highest under MT with MW (7.1 mg kg-1) and with MC (72 mg kg-1), respectively. The highest CEC at the end of winter from 2010-11 under MT in GW (3.67). Second year, ...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...treatment. Median lethal concentration (LC50) and median lethal time (LT50) values were calculated for each treatment using log-dose probit analysis. Results revealed a mortality response of termites directly proportional to the extract concentration for all treatments. The floral extracts of basil (O. basilicum) and Tecoma (T. stans) exhibited maximum termite mortality (i.e. 55.5 and 50.0%, respectively) with minimum LC50 a...

Muhammad Abdul Qayyum1*, Farhat Bashir2, Muhammad Mudassar Maqbool3, Anser Ali3, Saqib Bashir1 and Qaiser Abbas4 

...n because it has highest concentration of soluble salts. This study aims to examine the effect of NaCl salt at 100 mM and 200 mM concentrations on linseed (Linum usitatissimum L.) germination, plant growth, nutrients and soluble salts uptake. The results revealed that tested four linseed genotypes showed an overall germination rate of 86-94% at 100 mM NaCl (T2) and 78-84% at 200 mM NaCl (T3) and seedling survival rate of 80-...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...persons (15.2). Abnormal concentration of reactive oxygen species can disturb the body’s defense mechanism which cause potential oxidative injury to tissues and lead to cartilage degradation in RA patients.
...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...e, 5.5 pH, 5 mL inoculum concentration, 1.5 mL banana peel as substrate and 20 days incubation time. The antimicrobial activity of secondary metabolites was tested against Streptococcus aglactaie, Streptococcus pyogene, Escherichia coli and Proteus mirabilis. The fungus Aspergillus niger was found best among all tested strains as it produced maximum amount of antimicrobial agent Tautomycetin. The antimicrobial activities were determ...
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
..., pH 6.0 at 1% substrate concentration while MBL-GN02 produced maximum enzyme at 35oC, pH 4.0 with 0.5% substrate concentration. The MBL-SP02 produced maximum enzyme at 45oC, pH 7.0 with 2.0% substrate concentration. Among various carbon sources tested guar gum was the best producer of enzyme. Under these optimized conditions for each isolate, the best producers MBL-S...
Mehwish Faheem1*, Maleeha Rafeeq1, Aisha Majeed1 and Saba Khaliq2
...of acute exposure of low concentrations of BPA (10, 100, 1000 µg/l) on liver cyp1a1 and heat shock proteins mRNA level was evaluated using real time qPCR. Results revealed that exposure to bisphenol A caused decrease expression of cyp1a1 in a concentration dependent manner. Exposure to graded concentrations of bisphenol-A resulted in significant increase in mRNA express...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...the Group B, C, and D in concentration higher than 0.05 ppb. The AFB1 was excreted in milk as metabolite AFM1 @ 1.35-1.59%. Udder health and milk quality deteriorated as SCC and TVC increased. Levels of serum enzymes AST and ALT increased with ingestion of dietary AFB1. It is concluded that ingestion of very low level of AFB1 by lactating goats results in excretion of carcinogenic metabolite AFM1 in milk beyond the permissible level. Dietary AFB1 has role in s...
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
...step, minimum inhibitory concentration of test compounds was determined against bacterial species by using resazurin microplate assay. All the tested compounds exhibited variable antimicrobial properties against various test cultures. All the compounds showed stronger antimicrobial activity against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Compound III was found to be the most effective compound. These results demonstrate t...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...ed orally with different concentrations of levofloxacin (0.00, 9.37, 18.75 and 37.50µg/g BW of mice) for 30 days consecutively. Mice were sacrificed on 31st day (24 hours after last dose administered) under deep chloroform anaesthesia. Blood was extracted through cardiac puncture and viscera were dissected out for further analysis. Levofloxacin induced reduction in body weight while caused a noticeable increase in liver, kidney and testis weig...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...group (AD). A sub lethal concentration containing 15ug/g B.W of BP was given with and without turmeric orally, dissolved in corn oil, used as vehicle too. Dissections were made on 31st day, twenty-four hours after the last dose administration. Results showed that continuous administration of butyl paraben (BP) had decreased the organs weight as compared to control. Regarding biochemical parameters results had shown an increased enzymatic level of al...
Sumaira Abbas1, Muhammad Sultan Haider2,*, Fatima Kafayet1, Sana Ashraf2, Atifa Masood3 and Moazma Batool4
...re (1:1 v/v). Carotenoid concentration was determined by thin layer chromatography (TLC) and found satisfactory. By mixing fish meal, sunflower meal and rice polish four treatment diets, T1, T2, T3, were prepared and citrus peel powder was added @ 200g, 400g and 600g/ 1000g, respectively. While fourth one, control diet (To) was without citrus peels. These is nitrogenous diets (30% protein) were offered to Carassiu...

Hafiz Abdullah Shakir1*, Javed Iqbal Qazi2, Abdul Shakoor Chaudhry2, Muhammad Irfan3, Muhammad Khan1, Shaukat Ali4 and Saima Shahzad Mirza5 

...t; B > A. The highest concentrations (µg/g dry weight) of Cd (0.29), Cr (4.64), Cu (8.85), Fe (65.66), Mn (5.14), Hg (2.91), Ni (3.18), Pb (5.14) and Zn (72.16) were recorded at site C. Among the sampled fish species, C. mrigala showed highest potential of metals bioaccumulation than L. rohita and C. catla. Scales of the fishes caught during low flow season showed significantly (P<0.001) higher concentrations of ...

Waseem Abbas1, Shakeel-ur-Rehman2, Abdul Rashid2, Muhammad Kamran3, Muhammad Atiq2 and Muhammad Ehetisham ul Haq3* 

...ca were evaluated at 3 % concentration against eggs hatchability and adult emergence of the whitefly in lab condition. Two consecutive sprays were applied to assess the relative impact of different plant extracts against adult whitefly population and the disease incidence of cotton leaf curl virus disease in filed conditions. Azadirachta indica was found most effective to inhibit the egg hatchability and adult emergence as compared to other plant extracts appl...

Hassnain1, Abdul Basit1*, Mehboob Alam1, Imran Ahmad1, Izhar Ullah1, Noor Alam2, Inayat Ullah3, Muhammad Areeb Khalid1, Muhammad Shair4 and Noor ul Ain1 

...) environment. Different concentrations of chitosan (0, 50, 100,150 mg L-1) and various water stress intervals (3, 6, 9 and 12 days) were given to tomato plants from 15 days after transplantation. Results showed that various application of chitosan and water stress interval had a significant (P< 0.01) effect on almost all studied parameter. Tomato plants treated with 6 days water stress interval had maximum average plant height (82.69 cm), average numb...
Awais Bin Shahid1, Moolchand Malhi1,*, Saeed Ahmed Soomro1, Muhammad Giasuddin Shah2, Nazeer Hussain Kalhoro3, Asmatullah Kaka4, Rajoo Mal1, Muhammad Awais Soomro1Saba Parveen Samo1 and Muhammad Nawaz Sanjrani5
...aled that the molar concentrations of propionate and the total short chain fatty acid (SCFA) increased (P < 0. 005) and the pH decreased (P < 0.05) in the ruminal fluid of SY goats compared to C. The morphometry showed that the length, width and the density of papillae in the atrium ruminis, ventral rumen and ventral blind sac of rumen increased (P < 0.05) w...
Khursheed Ahmed, Shoaib Freed*, Rana Fartab Shoukat and Kanwar Waqas Ahmad
... with insecticides. Five concentrations i.e., 1×106, 1×107, 1×108, 2×108 and 3×108 conidia/ml of each isolate of entomopathogenic fungi were used in combination with insecticides against 5th instar nymphs of O. hyalinipennis. The combined treatment of isolates of entomopathogenic fungi and synthetic insecticides caused higher mortality of O. hyalini...
Hazrat Ali1,2,*, Ezzat Khan1,* and Muhammad Jamal Nasir3
...s designed to assess the concentrations of Cr, Ni, Cd and Pb in water, sediments and different fish species of River Shah Alam, a tributary of River Kabul in Khyber Pakhtunkhwa, Pakistan. The different environmental samples were collected from the river, downstream the city of Peshawar, and analyzed for the selected heavy metals by atomic absorption spectrophotometry. Heavy metal accumulation was studied in muscles of six fish species collected from the river....
Ammara Blouch1, Ata ul Mohsin2, Muhammad Naeem2 and Rashid Mahmood1*
...d with increase in their concentration and decreased with increase in larval instar. Similarly LC50 values suggested Turex (Bt sub species kurstaki and aizawai) to be more toxic with less LC50 values as compared to Dipel (Bt sub species kurstaki). These results indicated that these bio pesticides if used at early insect stage can help to control this pest. 
...

Noorullah Khan1*, Farrukh Siyar Hamid1, Fayaz Ahmad1, Sabaz Ali Khan2, Imtiaz Ahmed1, Muhammad Abbas Khan1, Shamsul Islam1, Abdul Waheed1, Basharat Hussain Shah1 and Hussain Shah

...ives to find the optimal concentration of IBA for better rooting and survival rate of seedlings from kiwi cuttings. The experiment was laid out according to randomized complete block design with three replications and 20 four budded branch cuttings of “Hayward” kiwifruit cultivar. The cuttings were treated with different concentration of IBA (0, 1000, 2000, 3000, 4000, and 5000 ppm) for ten second and then plante...
Hu Liu1,2, Qifang Yu1,2, Xiaopeng Tang1,2, Chengkun Fang1,2, Sijia Chen1,2 and Rejun Fang1,2,*
...e, egg quality, selenium concentration in eggs and serum antioxidant capacity in laying hens. Seven-hundred and twenty 21-week-old healthy Roman laying hens with a similar laying rate were randomly divided into 5 groups with 6 replicates of 24 hens. The experiment was designed by 2 × 2 factorial arrangement with two sources of Se and two levels of Se. The hens in control group were fed a basal diet without adding exogenous selenium source, and the hens i...
Umer Iqbal1,* and Tariq Mukhtar2
...arbendazim (79.11%) at a concentration of 150 ppm. Copper oxychloride at a concentration of 50 ppm gave the minimum inhibition (12.50%). Concentrations also had significant inhibitory effects on the growth of the fungus. All the fungicides caused maximum inhibition of the growth of the fungus at a concentration of 150 ppm. With a decrease in the

Ghulam Murtaza1*, Ghulam Sarwar1, Noor-Us-Sabah1, Mukkram Ali Tahir1, Fakhar Mujeeb2, Sher Muhammad3, Muhammad Zeeshan Manzoor1 and Ayesha Zafar1  

...in the reduction of salt concentration in the root zone. Organic matter was used to mitigate the negative effects of saline water irrigation using sorghum as test crop during summer, 2018. In this experiment water of 3 different types was applied coupled with addition of organic matter at 2 different rates. The experiment comprised of 9 treatments having 4 replications; T1 = canal water, T2 = saline water having EC value 2 dS m-1, T3 = saline water having EC v...
Monica Paula Marin1, Elena Narcisa Pogurschi1*, Iuliana Marin2 and Carmen Georgeta Nicolae1
...y metals, increasing the concentration of unsaturated acids (oleic, linoleic) and immunoglobulins in colostrum, as well as reducing the diarrheal gastric disease of cattle. By adding zeolite in the cow ration during the precalving period has determined a significant increase in blood serum calcium during the last week of precalving period, as well as in the calving and postcalving periods, having as consequence fewer recorded milk fever cases. The results obta...
Yan-Guo Han, Yu-Qing Han, Jia-Yuan Wu, Jia Guo, Ri-Su Na, Yan Zeng, Guang-Xin E, Yong-Ju Zhao and Yong-Fu Huang*
... growth factor 1 (IGF-1) concentrations were detected by radioimmunoassay. The offspring lambs in T group showed significantly lower serum SS and higher PRL concentrations than those in the control group, and there were no difference between T group and the control group in terms of serum GH and IGF-1 concentrations. There was a significant negative correlation between offspring lambs seru...
R. Yasmeen1*, S. Shaheen1, B.N. Khan2, S.S. Bokhari1, U. Rafi1 and A.W. Qurashi1
... water samples and small concentrations were noticed in soil samples. The amounts of Zn and Cu in feed were reflected in fecal samples; however, higher amount of Pb and Cd were noticed in fecal samples compared to feed, water and soil. This showed inhalation of these metals from the air. Amount of metals at different depths showed unique pattern low to high and again low that was irrespective of site location. Overall there was more exposure to air poll...

Nosheen Noor Elahi1, Muhammad Shafique1, Muhammad Imtiaz2, Umer Farooq3* and Muhammad Rashid1 

...creased at 20-100mM NaCl concentrations but drastically decreased at higher levels. While in varieties CM44 and CM2000 a gradual decrease of biomass with increasing salinity levels were observed. Number of pods, flowers and fresh weight of pods were not affected at salinity levels of 0-50mM and decreased at 100 mM NaCI in all the four varieties. In varieties CM91 and CM2000 nodules were formed at all levels of salinity treatments, while in varieties CM44 and C...
Yun-Feng Liu, Yong-Mei Guan, Dan Chen, Jia-Le Chen, Chen Jin, Wei-Feng Zhu, Lu Wu and Li-Hua Chen*
...skin and brain, the peak concentration (Cmax) and the area under the concentration-time curve (AUC0→t) of brucine and strychnine in co-administrated with PRAE were decreased significantly (P<0.05), and the Cmax and AUC0→t of paeoniflorin in co-administrated with SSE increased significantly (P<0.05). These results showed that the co-administrated of SSE...
Elmas Ulutaş1,*, Abdullah Eryavuz2, Aziz Bülbül2, Abdur Rahman3,*, İsmail Küçükkurt4 and Cangir Uyarlar5
...ptin and malondialdehyde concentration while other parameters remained unaffected. No difference was observed in ruminal pH between the treatment groups. Ruminal ammonia and number of protozoa were decreased (p<0.05) with 700 ppm and 1000 ppm zinc supplementation. Rumen Zn concentration increased (p<0.05) in the goats fed with 1000 ppm zinc, whereas there was no difference in rumen Fe and Cu co...
Nihat Yeşilayer*
...between total carotenoid concentration (TCC) and flesh colour, determined by visual and colorimetric methods, of three fish species. Linear relationships were observed between CIELAB colour measurements (lightness-L*, redness-a*, yellowness-b*, hue-H°ab and chroma-Cab), DSM SalmoFan card scores and TCCs. The redness (a*) was mostly correlated with TCC of the fish fillets. The FRT group had the highest TCC (9,75 mg kg-1) compared to the ot...
Tian-Yi Zhao1, Zi-Qing Liu2, Shu-Fei Ma3, Bo-Yang1, Fan-Fan Guo1 and Ming-Hua Duan1*

 

...;B signaling pathway and concentrations of TNF-α, IL-6, and IL-1β were measured. The results demonstrated that biatractylolide can reduce apoptosis in hippocampal cells, prevent and improve the cognitive decline induced by Aβ, prevent the morphological changes in hippocampal nerve cells and can reduce the activation of NF-κB signal pathway in vivo. Taken together, biatractylolide appeared to be a useful agent for the treatment of Aβ-...
Ju Zhang1,2, Dong-Sheng Jia3, Shi-Hao Zhao1,2, Xiao-Liang Xie3, Hong Chen4 and Hai-Feng Li4,*

 

...ild-type N2 nematodes at concentration < 0.6 mg/ml, and 0.05 mg/ml of the HGQ significantly prolonged the lifespan and reduced the lipofuscin level in N2 nematodes. However, the HGQ failed to influence the pharyngeal pumping rate in N2 nematodes or to affect the lifespan of sir-2.1 mutant nematode VC199, indicating the anti-aging effect of HGQ is independent of calorie restriction pathway. Further experiments showed that the HGQ decreased ROS and MDA...

Stanley Uchenna Onwudike* and Vivian Chizoba Edoziem 

...As a result of different concentrations of inorganic phosphorus forms within these roots zones, appropriate agronomic management practices such as crop rotation, mixed cropping and shifting cultivation is recommended for sustainable phosphorus management in the studied soil. 

...

Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui

...to establish the optimal concentrations of plant growth regulators and sucrose for micropropagation of sugarcane. Sugarcane is cultivated through cane sets and therefore, multiplication of any new genotype through traditional practices requires interminable period of time. Eight sugarcane genotypes viz. NIA-2004, SPF-234, NIA-2012, BL4, AEC92-1208, Thatta-10, Gulabi-95 and NIA-1026-P7 were subjected to tissue culture using four different combinations of shoot ...
Ahsan Numan1,4, Khadija Irfan Khawaja2, Abdul Basit Qureshi3Muhammad Shahbaz Yousaf4, Imtiaz Rabbani4, Hafsa Zaneb5 and Habib Rehman4*
...8-1.51) and triglyceride concentrations (OR:1.13, CI: 1.02-1.33). In conclusion, the DSP was considerably higher in DMT2 patients and was significantly associated with age of patients, duration of DMT2, HbA1c, blood fasting sugar and serum triglyceride concentrations. Proper management of DSP needs better management of DMT2.
...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...lpha;, IL-1β, IL-6) concentrations, urinary volume, creatinine, and both serum and urinary electrolyte levels were measured. In addition, the apoptosis rate of white blood cells was analysed from plasma samples. Tissue samples from the brain, heart, aorta and kidneys were used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF g...
Babar Khan1,2 *, Nazir Javed1, Sajid Aleem Khan1, Nasir Ahmed Rajput1, Muhammad Atiq1, Abdul Jabbar1, Abdul Rehman3, Anam Moosa1 and Muhammad Amjad Ali1*
...ssei. Four different concentrations(50, 100, 200 and 500 IJs) of tested EPN speciewere used against insect’s larvae. Reproduction rate of S. kraussei was highest at concentration of 500 IJs as compared to 50, 100 and 200 IJs. Similarly, the effect of different temperatures was also studied to evaluate the efficacy of S. kraussei on insect larvae. At 25°C, S. kraussei showed significantly hi...
Sumaira Akram1, Sajid Aleem Khan1*, Nazir Javed1 and Saeed Ahmad2
...gnificantly at different concentration of chemicals, bio control agents and plant extracts. Among chemicals, cartap was most effective with lowest egg hatching and among biocontrol agents Paecilomyces lilacinus (Thom) Samson (PL) produced highest egg hatching while, Trichoderma harzianum Rifai (TH) caused lowest egg hatching. In plant extracts the highest egg hatching was recorded in clove at S concentration an...

Asmaa A. Darwish 

...cerides, MMP-2 and MMP-9 concentrations was depicted in the three diseased groups. On the contrary, the total cholesterol, HDL-cholesterol, LDL-cholesterol, minerals, electrolytes, trace elements and total antioxidants capacity concentrations significantly (P< 0.05) decreased in the three diseased groups. Both of MMP-2 and MMP-9 yielded a sensitivity and NPV as 100% in the sheep pneumonia, arthritis and enteritis and corr...
Godwin Ikechukwu Ngwu1*, Maria Ifeyinwa Ngwu2, Fabian Chukwuemenam Okafor1, Aruh Ottah Anaga3 and Christopher Didigwu Nwani1
...ol. The results indicate concentration dependent mortalities and significant differences (P<0.05) in the mean percentage mortality between the laboratory-reared and field-collected Aedes mosquitoes. The mortality rate due to the extract was found to be less in the field collected A. aegypti (LC50 = 2.81 ± 0.14; LCI = 2.55; UCI = 3.11) than the laboratory-reared ones (LC50=1.24±0.09; LCI = 1.07; UCI = 1.43) a...
Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Muhammad Ibrahim Khaskheli2, Gul Bahar Khaskheli3, Allah Jurio Khaskheli2 and Arshad Ali Khaskheli1
...> pertained considerable concentration of crude protein, Zea mays high ether extract, Calligonum polygonoides beared significantly high level of carobohydrate.
...
Meriem Msaad Guerfali1,*, Heitham Hamden1, Salma Fadhl1, Wafa Djobbi1, Lotfi Sillini1, Wafa Marzouki1 and Mohammed Ammar2
...nd spinosad at different concentrations. Histopathological effects of spinosad were also studied and showed that for doses up to LC50, annoying effects begin to appear in glial and neurosecretory cells. The LC50 for malathion was 75.61 ppm for the populations treated more than 6 times. Low levels of...
Seval Dernekbasi* and Emin Karatas
...had significantly higher concentrations of n-6 fatty acids, predominantly in the form of linoleic (LA, 18:2n-6c) and oleic acid (OA, 18:1n-9c), while n-3 fatty acids were present in significantly higher concentrations in the FO group. The fatty acid composition of rainbow trout fillet reflected the dietary lipid source. Fillet of fish fed by the FO diet had highest eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic aci...

Hamza Rehman*, Nida Ahmad Khan, Asad Ali, Warda Batool and Kanwal Razzaq 

...tested at four different concentrations (0.25, 0.5, 1 and 2ppm). Mortality of insects was documented after 24, 48 and 72 hours. Abbot’s formula was used to determine the corrected mortality. At 2ppm concentration thiamethoxam and imidacloprid provided 87.57% and 78.59% mean mortality of khapra larvae on treated filter paper after 72 hours respectively. On treated wheat, thiamethoxam provided 82.61% while imidacloprid g...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
...ion after slaughter. The concentrations of 4-methyloctanoic acid and 4-methylnonanoic acid in waist subcutaneous adipose tissue were detected using gas chromatography. Immunisation of oral and plasmid-injected KISS1 DNA vaccines induced strong antibody response, significantly suppressed testosterone secretion and reduced scrotal circumference and testis weight. It also significantly reduced the concentrations of 4-met...

Pushpa1, Nighat Seema Soomro1, Shahla Karim Baloch2, Mehmooda Buriro1, Aijaz Ahmed Soomro1, Muhammad Tahir Khan3, Qamar Uddin Jogi1*, Muhammad Nawaz Kandhro1 and Farheen Deeba Soomro1 

...nverse proportion to the concentration of allelopathic weed powder. Shortest shoots (14.46 cm) and roots (3.20 cm) were seen in variety TJ83 at the highest concentration of Avena fatua powder (75g kg-1 soil). The minimum shoot fresh weight (0.13 g), root fresh weight (0.10 g), shoot dry weight (0.04 g), and root dry weight (0.03 g) were also noticed in variety TJ83 under the highest treatment of Avena fatua powder (75g kg-1 ...

Rehman Ali Keerio1, Nighat Seema Soomro1, Aijaz Ahmed Soomro1, Mohammad Aquil Siddiqui2, Muhammad Tahir Khan2, Ghulam Shah Nizamani2*, Muhammad Nawaz Kandhro1, Maryum Siddiqui4, Hajra Khan3 and Farheen Deeba Soomro

...vely on applying highest concentration of Zn. While, seeds weight head-1, seeds index, seed yield, and oil content ascended by 76.6%, 37.2%, 81.4%, and 43.2%, receptively as compared to the control. It was concluded on the basis of these findings that the foliar application of Zn in 2.0% concentration can be employed to increase the sunflower yield and oil content. The study suggests that Zn can be employed for improving sun...

Ahmad Naeem Shahzad1, Muhammad Kamran Qureshi2, Samee Ullah1, Muhammad Latif3, Shakeel Ahmad1 and Syed Asad Hussain Bukhari1* 

... in cotton plants. Three concentrations of trehalose (0, 5 and 50 mM) were applied to plant foliage, subjected to varying levels (0, 11 and 17 dSm-1) of salinity. Salt stress disturbed the sodium and potassium concentrations in leaves and roots of plants. Different morphological traits of plants including number of leaves, leaf fresh weight, shoot fresh weight, root fresh weight, plant height, leaf dry weight and root dry we...

Tahira Moeen Khan*, Irum Riaz, Shahida Hameed and Bushra Khan 

...aking 25ppm/50ml initial concentration of NaAsO2. Best removal (90%) was achieved for Lemon juice microwave activated date seed husk LMDS (time= 30mins, agitation speed= 150 rpm, pH= 5, adsorbent dose= 0.1 g). While 85% removal efficiency was observed for Raw date seed husk RDS (time= 45mins, agitation speed=250 rpm, pH= 4 and adsorbent dose= 0.5 g). The adsorption isotherms (Freundlich and Langmuir) and adsorption dynamic kinetic studies were also conduct...
Hifzulrahman1, Muhammad Abdullah1*, Muhammad Uzair Akhtar2, Jalees Ahmed Bhatti3, Muhammad Saadullah1 and Muhammad Naveed-ul-Haque2
... treatment increased the concentration of milk fat and milk fat yield by 3.5 and 11.2%, respectively, compared with the Ctrl. However, the Ca-FA treatment did not change the plasma urea nitrogen, glucose, and triglyceride, compared with the Ctrl. Similarly, the reproductive indices were not different between Ca-FA treatment versus Ctrl. In conclusion, the Ca-FA are useful to increase milk production and milk fat yield without effecting the plasma metabolites a...
Renlong Lv1*, Ken-Ichi Horiguchi2, Shin-Ichi Tagawa3, Shoichi Ichihara4 and Norio Yoshida
...tal volatile fatty acids concentration between two experimental diets, and the palatability of jatropha meal showed to be bad. However, the blood parameter and rumen fermentation did not show any abnormal value after using jatropha meal of 5% and 10% of concentrate in sheep.
...
Saeed Fatima1, Khalid Javed Iqbal1, Usman Atique2,3*, Arshad Javid4, Noor Khan2, Sonia Iqbal2, Hamid Majeed5, Hamda Azmat2, Bakhat Yawar Ali Khan6, Irfan7, Muhammad Tausif Shahid1, Gulnaz Afzal1
...ed the pattern of metals concentration in fish in the order of Cd > As > Hg. However, R. rita, O. niloticus and C. idella showed higher accumulation of the metals that clearly alluded to species-linked metal bioaccumulation, which might be due to their unique feeding mood. Cadmium presence ranged between 0.04 – 1.12 ppm while As and Hg fluctuated between 0.01 – 0.04 and 0.001 - 0.18 ppm, respectively. Conversely, higher b...
Asim Faraz1*, Abdul Waheed1, Annamaria Passantino2, Ayman Balla Mustafa3, Nasir Ali Tauqir4, Naeem Ullah Khan5, Muhammad Shahid Nabeel6
...level of Hb (hemoglobin) concentration (P<0.05) was found to be significantly different as 16.4±0.14 and 16.8±0.09 (g/dL) with G1 and G2, respectively. The concentration levels of cholesterol, triglycerides, urea, creatinine, albumin, total-protein, glucose, Ca and P were found significantly (P<0.05) varied among groups. Regarding hair mineral profile, concentrations of...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
...fat, lactose and mineral concentration are influenced by exogenous OT administration. It affects the cell maintenance and mammary metabolism along with its proven physiological role in the milk ejection reflex. OT effects are not manifested through effects on cell remodeling. Observed effects of OT administration on reproductive anomalies are anestrous, the development of corpus-luteum cysts, follicular ovarian cysts, delayed age at puberty, repeated estrous c...
Min Lv1, Hui Gan2, Zhide Ruan1, Huizan Yang1, Rui Wang1, Laiba Shafique3, Huma Naz4 and Huawei Ma1,
...m) with dissolved oxygen concentrations above 5.2 ± 0.3 mg/l, and pH 6.5–8.1. Water flow in the ponds was poor. The amount of feed increased from 0.81 ± 0.35 to 13.42 ± 1.89 g/fish-day throughout the experiment. Samples were collected between mid-March and October 2016, and we monitored the Cd, Cr, and Hg concentrations in gills, large intestines, small intestines, intestine
Yan Xu1, Jia Zhou1, Guangfu Lv2, Yuexin Liu1, Xintong Zhao1, Xin Li1, Doudan Ye2, Xiaobo Qu1,* and Xiaowei Huang1*
...o experiments. Different concentrations of DOX were used to treat H9c2 cells for 6, 12, 24, and 48 h, respectively, to determine the IC50. Different concentrations of PAP were treated to H9c2 cells for 24, 48, and 72 h to determine the effect of PAP on H9c2 cells. The experiment was divided into control group, DOX group, PAP group and DOX+PAP group. Lactate dehydrogenase (LDH) leakage and creatine kinase MB (CK-MB...

Asmaa Abdallah Darwish 

...udies investigated their concentrations in different sheep diseases. So, this paper aimed to assess the antioxidant vitamins (E, A, D3 and C) levels in some sheep physiological and pathological conditions. For this purpose, 200 barki ewes were divided into control group, inflammatory diseases groups (pneumonic group, arthritic group, enteric group), late pregnant groups (normal late pregnant group, pregnancy toxemia group), postpartum groups (normal postpartum...
Khayyam1*, Muhammad Zahid1, Naiz Ali2 and Qadeem Khan3
... and Luteinizing hormone concentrations were higher than normal range in both male and female Grave’s disease patients. Prolactin and serum gonadotropin showed fluctuations in Hashimoto’s thyroiditis patients but in Grave’s disease patients only the gonadotropins showed abnormal values while prolactin was in the normal range. The study suggests that autoimmune thyroid diseases effect the pituitary-hypothalamic axis which needs to be further i...
Afzal Nimra1, Zulfiqar Ali1*, Safdar Sidra 2, Rida Ahmad1
...This study discusses the concentration and possible sources of airborne trace metals in sensitive areas of hospitals, which is to our knowledge, is the first study contributed from Pakistan and its understanding is important in order to improve law enforcement against causative factors. 
...

Muhammad Salim1*, Ayhan Gökçe1, Muhammad Nadir Naqqash1 and Orkun Ersoy

...treated with the highest concentration (2000 mg/Kg). At the highest concentration (2000 mg/Kg), the mortality reached as high as 100% after 2 weeks, while the lowest significant percent mortality (1.00±1.00%) was recorded in the control one week after exposure and only increased to 3.53±0.00% after 3 weeks. Percent weight loss in grain was also determined after 21 days. A significantly higher percent weight los...

Paul Oludare Adetunji* and Safiriyu Idowu Ola 

...ers (semen volume, semen concentration, sperm output, sperm motility and sperm abnormalities) from 26-52 weeks of age and sexual ornaments’ morphometry (Comb Length-CL, Comb Height-CH, Wattle Length-WL, Wattle Height-WH and Inter Pubic Distance-IPD) from 16-24 weeks of age. Data on onset of puberty and semen quality parameters were subjected to Analysis of Covariance (ANCOVA) with body weight at puberty as a covariate while morphometry measurements were ...

Ehsan-Ul-Haque1, Akbar Hayat1*, Muhammad Asim1, Sajjad Hussain2, Muhammad Shakeel Hanif3, Muhammad Zubair1, Muhammad Abdullah Jamil1 and Faheem Khadija1 

...ate incorporated (6% w/v concentration) in wax were used to compare with commercially used fungicide (Thiabendazole). Study was conducted at 4±1 C° with 90±05% relative humidity for 60 days followed by one week of storage at ambient conditions. Least decay incidence 1.2% and 1.8% respectively in Kinnow and Willow leaf was observed in fruit treated with fungicide in wax followed by fruit treated with Potassium sorbate in wax (2.8% and 3.6%). T...

A. S. M. El-Nuby1† , S. A. Montasser2 and I. A. El-Khadrawy

Control of root-knot nematodes using wild plants colonized Sinai, Egypt
...acantha (60.4%) at stock concentration. The addition of extracts as soil drench was better than spraying them. The stock solution showed the highest reduction in nematode reproduction but the differences between 100% and 75% concentrations were non-significant. Our finding offers a non-poisonous tool that can insert in combating nematode programs especially for small-holder growers colonized Sinai Peninsula.

...

 Tajwali Khan1*, Ihtzaz Qamar1, Feroz Shah2, Kareem Akhtar2, Rehman Akhtar3

MODEL FOR FUEL DROPLET EVAPORATION IN COMBUSTION CHAMBER OF LIQUID PROPELLANT ROCKET ENGINES
...engine where fuel
concentration away from droplet is not zero. A code is generated and applied. The results of the model show that
fuel droplet diameter, combustion chamber pressure, chamber diameter, mass flow rate and droplet relative velocity
with hot gas has significant effects on the life of droplet and characteristic length. Effects of chamber pressure and
diameter are more significant for larger droplet size.

...

Daulat Khan1*, Khizar Azam2, Noor Muhammad Khan1, Muhammad Israr1, Naeem Ejaz3 

ASSESSMENT OF WASTEWATER FOR DISPOSAL IN ACCORDANCE WITH NEQS CRITERIA AND ITS REUSE FOR IRRIGATION WITH DILUTION
...t or waste in an amount, concentration or level which is more than the
National Environmental Quality Standards(NEQS)”. Even in the presence of this act there are many housing societies
and Industrial zones, which are continuously disposing off their untreated wastewater to the natural water bodies. In
this study Laboratory investigations on 5 composite samples were carried out. Total of 19 parameters were analyzed
including T...

Muhammad Shahzad Khattak*1, Barkatullah1, Ayesha Aziz1, Jamil Ahmad2, Mohammad Sharif3, Mukand Singh Babel4, Muhammad Fahad5

IMPACTS OF CLIMATE CHANGE ON CROP WATER REQUIREMENT UNDER MULTI - REPRESENTATIVE CONCENTRATION PATHWAYS DURING MID-CENTURY: A CASE STUDY OF D. I. KHAN

Shabana Rafique1, Shahnaz Parveen Khattak1, Tanveer Hussain2, Faiza Tauqeer1, Bashir Ahmad3 

EVALUATION OF FLEXURAL RIGIDITY AND ABRASION RESISTANCE OF POST AND META-FINISHED PIGMENT DYED P/C FABRICS
...ers i.e. finish type and concentration were taken in to consideration along different modes
of application on the abrasion resistance and flexural rigidity of fabrics. As regards the type of finish, the effect of
U-V absorber and Pekoflam OP flame retardant (organic phosphorous compound) on fabric stiffness was found to be
advantageous, providing it a soft texture. The post-application of fluorine based liquid water repellent finish (NUVAF...

Hamza Tariq1, Imran Hayat1, Saima Rafiq1*, Aqsa Qayyum2 and Sohrab Qayyum1 

...rved with increasing the concentration of autumn olive in the beverage blends while a decreasing trend was observed for pH, reducing sugars, non-reducing sugars and total sugars. Organoleptic parameters such as color, flavor, taste and overall acceptability significantly (p < 0.05) influenced. The TSS, acidity, reducing sugars and total sugars content increased whereas pH, ascorbic acid, antioxidant activity, total phenolics and non-reducing sugar content d...

Sajjad Haydar*, Zunaira Asif*, A. A. Bhatti**, Obaidullah Nadeem***, Ghulam Hussain*, Nadeem Abbas*

EFFICIENCY OF WASTEWATER TREATMENT PLANT IN THE PUNJAB TANNERY SECTOR: A CASE STUDY OF DADA TANNERY
...w wastewater showed high concentration of organic matter
and Chromium. Phosphorous concentration was found deficient for satisfactory biological treatment. WWTP showed
overall removal efficiency of 88.81, 84.54 and 62.31% for total suspended solids (TSS), five day biochemical oxygen
demand (BOD) and chemical oxygen demand (COD), respectively. Whereas, the mean effluent

 Muhammad Irfan*, Saeed Gul*, Mohammad Younas*, Babar Nawaz*, M. Ishfaq*, M. Amir Durrani*, Abid khan*

FERMENTATION OF SUGARCANE MOLASSES BY SACCHARAMYCES CEREVESIA: EFFECTS OF OPERATING PARAMETERS ON ETHANOL PRODUCTION
... time, molasses solution concentration and its pH during ethanol recovery from sugarcane molasses has been investigated. The interaction effects, optimum conditions, and percent contribution ofeach factor were determined statistically. The maximum etha...

Nisar Mohammad*, Mohammad Mansoor Khan* Noor Mohammad*

CHARACTERIZATION OF TALC-CARBONATE ROCK OF MINGORA EMERALD MINES, DISTRICT SWAT, KHYBER PAKHTUNKHWA, PAKISTAN
...ch characterization/ pre-concentration investigations study was done for low-grade talc- carbonate rock of Mingora emerald mines located in District Swat, Khyber Pakhtunkhwa (KP),

Shahida Nasreen Zakir*, Samina Siddiqui**, Nasreen Ghaffar*

MAJOR ELEMENTS CONCENTRATIONS IN CALCAREOUS SOILS
...luate the major elements concentrations in soils derived from various parent materials and then to classify such soils in calcareous, moderate and non-calcareous soils. Soils were analyzed for total content of Al, Ca, Fe, K, Mg, Na, P, Si and Ti with Atomic Absorption Spectrophotometery and UV-VIS Spectormetry. Total Ca content in loess soils is 12% thus categorized in calcareous soils whereas alluvium and piedmont alluvium soils with Ca content ranged from 0....

Hazrat Hussain*, Muhammad Ibrahim**, Navid Ahmed***, Zia ul Haq**, Bashir Alam***

ASSESSMENT OF SEDIMENT TRANSPORT LOAD FROM GOAML RIVER AND ITS IMPACT ON THE GOMAL ZAM DAM LIFESPAN
... sediment load, sediment concentration and sediment quality have been estimated from the actual data and are shown by histograms and bar graphs. The relations developed are applicable only to the Gomal River system. It is hoped that the study will invite interest and attention of planners, researchers, and all those concerned with the problems and menace of soil erosion, sediment load concentration in hyd...
Zarish Yaqoob1, Saleema Bashir Shams2, Gaitee Joshua2 and Bibi Nazia Murtaza2,3*
...sent study describes the concentration of heavy metals in the water and different tissues of native fish collected from three different areas. The highest concentration of Cr (1.451 µg L-1) and Co (0.325 µg L-1) was measured in the river water at Balloki. However, Cd (0.981µg L-1)and Zn (1.239 µg L-1)were contributing maximum contamination at confluence of Deg Nallah a...

 Amir Naveed1, Unsia Habib1, Fouzia Parveen2, Syed Naveed Ul Hasan1, Muddasar Habib1, Irfan Shah1

AN INVESTIGATION OF HEAVY AND TRACE ELEMENTS IN COAL DEPOSITS OF MAKARWAL PAKISTAN AND THEIR POSSIBLE IMPACTS ON SURROUNDING WATER-CASE STUDY
...oal samples had the high concentration of Ni, Cr, As, and Cd. However, the coal samples from Mk-C had higher contents of Cu and Pb. Whereas, Fe was abundant in Mk-B coal samples. These results can be used as a helping tool for the prediction of environmental risks associated with coal mining in Makarwal area. Further, the water samples of Makarwal area were also analyzed and found to be contaminated with heavy metal pollutants.

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Hina Imtiaz1*, Salma Khalid2, Kashaf Riaz1, Muhammad Arshad Ullah3 and Zubair Rehman

...ssessment of heavy metal concentrations i.e. Lead (Pb), Copper (Cu), Nickel (Ni), Chromium (Cr), Zinc (Zn), Mercury (Mg) and Cadmium (Cd) were carried by Atomic Absorption Spectrophotometer. Significant concentrations of heavy metals were detected except Cu, Ni and Zn. Assessment regarding the types of waste generation, diseases among the residents and their opinions about the recycling and proper waste management were carri...
Fang Liu*
...e show that FGF10 exerts concentration dependent functions in angiogenesis and hypothalamic axon guidance. At lower concentration of 100ng/ml, FGF10 promotes angiogenesis in chorioallantoic membrane endothelial capillaries (CAMs), but FGF10 does not alter the endothelial growth significantly at higher concentration (500ng/ml, P>0.05). Simultaneously, FGF10 exerts a graded action, at low...

Ghulam Murtaza1, Ghulam Sarwar1*, Muhammad Ashraf Malik2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1 and Sher Muhammad3 

...C, and SAR and enhancing concentration of organic matter, phosphorus and potassium. However, T3 (water of EC 3 dS m-1) increased soil electrical conductivity, pH, SAR, and lowered organic matter, phosphorus and potassium concentration. Data were statistically analyzed by statistix 8.1 ANOVA approach along with Tukey’s test (HSD) at probability level of 5% for comparing treatments significance.  

...

Haiyan Yang1, Hongxia Liu1, Wenlong Wu1*, Weilin Li2 and Lianfei Lyu

...lectrolyte leakage (EL), concentrations of photosynthetic pigments, protein, soluble sugar, hydrogen peroxide (H2O2), malondialdehyde (MDA), ascorbate (AsA) and reduced glutathione (GSH), activities of superoxide dismutase (SOD) and peroxidase (POD) in leaves of ‘Hull Thornless’ were investigated. In the treatment group, water stress significantly increased EL, and the accumulation of photosynthetic pigments, protein, soluble sugar, H2O2 and MDA. A...

Syeda Farzana Bibi* and Siraj-ud-Din

Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay
...activity. At higher concentration (100-1000 μg/ml) the leaf and bark ethanolic extracts showed cytotoxicity and at lower concentration (10 μg/ml) very low cytotoxic effect was observed (Figure 4). The LC50 values for crude leaf extract were found to be 5.34 and for that of stem bark extract, it was 7.44 ug/ml. This study manifests that the selected plant can be helpful in the preparation of novel ph...

Kamran Ghasemi1*, Mostafa Emadi2, Asghar Bagheri3 and Mehdi Mohammadi1

Casing Material and Thickness Effects on the Yield and Nutrient Concentration of Agaricus bisporus
...phosphorus and potassium concentration of treated mushrooms but nitrogen concentration was affected by casing layer soil materials and thickness. The lowest nitrogen concentration was in 4 cm depth of CP casing layer that was not significantly different with 2 cm depth of CP casing layer. As a general conclusion, CCS in depth of 4 cm can be the best choice for casing but in ...

Mazhar Abbas1*, Kishwar Jam1, Rashid Iqbal Khan1, Muhammad Zafar-ul-Hye2, Tariq Rafique3 and Zahid Mahmood

...lant products at variant concentrations showed an increase of antioxidant activity by upgrading superoxide dismutase activity (64.42 U mg-1 protein), peroxidase activity (1.90 µmol min-1 g-1 protein), catalase activity (37.81 nmol-1 g-1 protein) and protein (6.28 mg g-1 fresh weight) contents. These commercial plant products based on amino acids enriched macro and micronutrients as well as acetyl-salicylic acid combined with ascorbic acid can be used car...
Anum Feroz1, Abdul Rauf Shakoori2 and Farah Rauf Shakoori1*
...xic effect of sub-lethal concentration (LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain (Lab-S) and deltamethrin resistant population (GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the a...
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2,  Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
...s purpose, two different concentrations 30 µl and 60 µl crude as well as ethanol extract of Aloe Vera were used. During this experiment, total 50 wound samples were collected from donkey wounds. All 50 samples were found contaminated with different bacterial organism, among all those 45, 35, 33, and 25 were recorded positive for S. aureus, E. coli, Shigella and Salmonella spp respectively. However, out of 50 sample, 34 (68%) were foun...

Muhammad Ismail1, Tufail Ahmad2, Shamsher Ali2*, Shahid Ali1, Nur Ul Haq1 and Naveedullah3

Heavy Metals (Pb and Ni) Pollution as Affected by the Brick Kilns Emissions
... samples showed that the concentration of  Pb and Ni in chimney A and B as 2.90 and 1.8, 13.11 and 14.7 mg kg-1 respectively. The concentration of Pb and Ni at this stage is not in toxic range however with the continuous addition may pose a severe threat in future. Similarly, soil sample analysis revealed AB-DTPA extractable Pb and Ni for chimney A and B as (0.11- 0.13, 14.2 and 15.0 mg kg-1) respectively. Here again th...

Adnan Arshad1*, Huma Qamar2, Ristina Siti-Sundari3, Zhang Yue1, Muhammad Zubair2, Muhammad Ali Raza4, Muhammad Habib-ur-Rehman5 and Lizhen Zhang1*

Phenotypic Plasticity of Spineless Safflower (Carthamus tinctorius L.) Cultivars in Response to Exogenous Application of Salicylic Acid under Rainfed Climate Conditions
...lower cultivars and five concentrations of salicylic acid (SA) in three replications was conducted. The safflower promising genotypes viz; C1 (L16358), C2 (L16378), C3 (L26748), C4 (L26754), and C5 (L16385) were tested against SA1: 0 mM, SA2: 0.25 mM, SA3: 0.50 mM, SA4: 0.75 mM, and SA5: 1.00 mM salicylic acid concentrations. The result indicated that most responsive cultivar to rainfed conditions was C3 (L26748) under SA4 (...

Safina Naz1*, Muhammad Akbar Anjum1, Bushra Siddique2, Syed Atif Hasan Naqvi3*, Sajid Ali1, Hassan Sardar1, Sakeena Tul Ain Haider1, Muhammad Asif Zulfiqar4, Hajra Azeem3, Sibghat Ullah5, Shah Pasand6 and Zehri Khan5

Effect of Sewage Water Irrigation Frequency on Growth, Yield and Heavy Metals Accumulation of Tomato and Okra
... revealed that the metal concentration high in tomato and okra plant parts with decreasing irrigation intervals of sewage water.

...
Chao Zhao1,2, Sigang Fana2 and Lihua Qiua2,3,*
...ia, nitrite, and nitrate concentration), heterotrophic bacteria, and vibrios during different P. monodon stages(nauplii, zoea, mysis stage, postlarvae). In ponds 1, 2, 3 and 4, the survival rates were significantly lower but the nitrite concentration and vibrio numbers (before mysis stage) were considerably higher than those in other ponds (P < 0.05), indicating that nitrite and vibrios influenced P....

Amit Sharma1* and Pankaj Sood2

Sonographic Assessment of Ovarian Follicular Dynamics during Breeding and Non-Breeding Season in Gaddi Goats
... season. Average mean P4 concentration was 0.30±0.04 ng/ml throughout the period of observation during NB whereas, peak P4 concentration of 11.89±1.55 ng/ml was observed at Day 14 of estrous cycle during B season. Significant difference between average daily counts of small, medium along with daily total number of visible follicles were observed during B season. In conclusion, ovarian follicular growth in Gaddi...

Abdul Jalal, Abdur Rab* and Naveed Ahmad

Aloe Vera Gel Coating Retains Persimmon Fruit (Diospyros kaki) Quality during Storage at Room Temperature
...ations and Aloe vera gel concentrations significantly decreased the loss of persimmon fruit quality during storage at room temperature. Storage at room temperature (26±2 °C) resulted in significant decline in fruit firmness, percent acidity, and ascorbic acid content with a concomitant increase in total soluble solids (TSS), organoleptic quality (Taste) score and decay incidence. Coating of persimmon fruits with 30% Aloe vera gel retarded the storag...
Huma Abbas1, Muhammad Azhar Iqbal2, Muhammad Kamran3, Muhammad Umar Shahbaz3*, Haseeb Ullah Kamber1, Nazir Javed1, Muhammad Junaid1, Hira Abbas4 and Muhammad Ehetisham ul Haq3
Evaluation of Advanced Mung Bean Germplasm against Cercospora Leaf Spot and its In-vitro Management by Different Fungicides
...10, 50, 100, and 200 ppm concentration against C. canescens using poisoned food technique. Score at 100 ppm was found most effective (93.03%) against the disease as compared with other fungicides. From present research, it is devised that 16051 line may be used for future breeding program and Score at 100 ppm may be evaluated for field trials in future studies.

...

Shahid Nadeem1*, Taj Naseeb Khan1, Mushtaq Ahmad1, Adnan Younis2 and Iram Fatima3

Influence of Plant Growth Regulators by Foliar Application on Vegetative and Floral physiognomies of Gladiolus
...ngth (77.67cm) at 1.75mM concentration of GA3. The Effect of SA found worthy as it enhanced the floral growth and decrease the number of days towards blooming. Moreover, the days to spike emergence, days to bud color and days to floret open were decreased (96.47, 101.20 and 105.77 respectively) with 1.75mM salicylic acid. However, the results showed the positive impact of GA3 and SA on vegetative as well as floral physiognomies of gladiolus plants.

...

Zahir Shah1*, Saima Munawar1, Ihsan Ullah2, Taif Shah3, Haq Aman Ullah1, Saqib Nawaz1, Farhan Anwar Khan1 and Manoj Kumar Shah4

Combined Physico-chemical and Analgesic Effects of Electroacupuncture Plus Clonidine in Goats
...ntly lower serum glucose concentration was noted in EA plus 10µg/kg of clonidine treated goats at 15-60 min than those treated with clonidine alone. Creatinine, blood urea nitrogen, alanine/aspartate aminotransferase activities, and hematological variables for differently treated groups were insignificant. In conclusion, EA combined with a low dose of 10µg/kg of clonidine provided better analgesia in experimental animals.

...

Tahseen Ullah* and Noor ul Amin

In Vitro Antibacterial Activity of Medicinal Plant Extracts
...dford, UK. Two different concentrations (low, 5 mg/ml and high extracts, 300 mg/ml) of these plants were evaluate against two different bacterial strains i.e. Bacillus subtilis and E. coli using disc diffusion method to uncover antibacterial potential of the plants extracts. The antibacterial investigation was performed in three replicates using disc diffusion technique using Nutrient Agar Media for the growth of B. subtilis and E. coli bacteria. The ANOVA for...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...0%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at 4&d...
Gulzhan Aubakirova1*, Zhanat Adilbekov2, Assylkhan Inirbayev2, Assel Zhamanova2 and Aibar Akhmetov3
...s within the permissible concentration, however, a decreased permanganate oxidation was noted, which indicates a satisfactory state of water quality. Widespread infection of fish with postdiplostomatosis was revealed in assessing fish safety; in rare cases, diplostomosis and ligulosis were detected. The incidence of fish with bacteriosis was not observed, with the exception of isolated cases of aeromonosis. Exceeding the maximum permissible
Aatif Amin1,*, Zakia Latif2, Arslan Sarwar1, Basit Zeshan1 and Mushtaq A. Saleem1
...ce level against various concentrations of HgCl2. Bacterial isolatesAZ-1, AZ-2 and AZ-3 showed resistance up to 40 μg/mL of HgCl2 and mercury sensitive (HgS) isolate ZA-15 was taken as a negative control. 16S rDNA ribotyping and phylogenetic analysis were performed for the characterization of isolates as Bacillus sp. AZ-1 (KT270477), B. cereus AZ-2 (KT270478), B. cereus AZ-3 (KT270479) and Ente...
Asim Faraz*
...a, Mg, Cu, Zn, Fe and Mn concentrations were found to be significantly different (P<0.05) among calf groups in IMS, SIMS and EMS.
...
Abida Parveen1, Sajid Aleem Khan1, Nazir Javed1, Anam Moosa1*, Khushboo Javaid1, Hina Safdar1 and Asma Safdar2
...th SK at three different concentrations and time intervals. After 12-h ‘S’ concentration of aqueous MOextract + ethanolic MO extract + SK produced 100% juvenile mortality while the treatment MO + SK caused the least juvenile mortality of MI. Juvenile mortality was increased in a time-dependent manner. Aqueous MO extract + ethanolic MO extract + SKproduced 100% egg hatching inhibition at standard ‘S’ <...

Dalal H. Sary and Rama T. Rashad*

A Comparative Study on the Impact of Compost, Humate, and Silicate on the Nutritional Characteristics of Calcareous Soil Cultivated by Soybean
...ave almost decreased the concentration (mg kg-1) of Cu, Fe, Mn, Zn, and Si in soybean seeds significantly by increasing the application rate from 50 to 100 %. Potassium silicate was the most effective Si-source in this study due to its content of readily soluble Si in soil solution. Silicon uptake can partially control the availability and uptake of some nutrients from soil. Soil may be enriched by versatile advantages upon application of K-H and K-Si but the ...

Asif Ali Abro1* and Iqbal Ahmed Panhwar2

Impact of Various Factors on Crop Diversification Towards High Value Crops in Pakistan: An Empirical Analysis by using THI
...ed to check the level of concentration or diversification in the structure of crops in Pakistan. The values of the Herfindahl index obtained as a result of the analysis indicate a high level of crop concentration and need to be diversified towards high value crops. The ARIMA time series model (1,1,1) was used to study the impact of various factors on the Transformed Herfindahl Index (THI) of diversity. To check the impact of...

Safiullah1, Azmat Ali Awan2, Mohammad Ilyas3, Fahim Ullah Khan3, Arshad Iqbal3*, Muhammad Adil3 and Asad Ullah1

Effects of Different Concentrations of Naphthalene Acetic Acid on Rooting of Various Olive Varieties
...dy the effect of various concentrations [0 ppm (control), 1000 ppm, 2000 ppm and 3000 ppm] of Naphthalene Acetic Acid (NAA) on rooting of various olive varieties (Hamdi, Nocellara and Sevallino) an experiment was conducted at Agricultural Research Institute (ARI) Tarnab, Peshawar during 2016. The trial was performed using two factorial RCBD. Cuttings were collected from mature olive orchard. The cuttings were treated for ten seconds in required
Wajahat Azeem1,*, Tariq Mukhtar1 and Tooba Hamid2
... was the maximum at 100% concentrations of both the agents while the minimum inhibition and mortality was obtained at 25% concentration. No statistical difference was observed between T. harzianum and A. indica in causing hatching inhibition and larval mortality. Numbers of galls, egg masses and reproductive factor were reduced significantly as a result of A. indica and T. harzianum applications. ...

Muhammad Nazar1, Chaudhary Muhammad Ayyub2, Mujahid Ali3*, Malik Abdul Rehman4, Shoib ur Rehman5 and Saqib Ayyub2

Amelioration of Thermo-Tolerance Ability in Spinacia oleracea L. Cultivars by Foliar Application of Chitosan
...application with optimum concentration may help to improve the production potential of spinach cultivars in areas hit by temperature stresses.

...
Shakeel Ahmed* and Sarwat Jahan
...tervals. Plasma T and GH concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly (P<0.001) decreased plasma T levels but it did not cause any significant (P>0.05) change in plasma GH levels compared to normal fed monkeys. NMS injection induced a significant increase (P<0.05) in T and GH concentrations compared to saline treated animals suggesting the possible involv...

Syeda Farzana Bibi* and Siraj ud Din

Unraveling the Bioherbicidal Potential, Elemental Analysis and Nutritional Evaluation of Crataeva adansonii Dc Leaf and Bark
...inhibition at 1000 μl concentration, and at the same concentration, the bark extract inhibited the frond growth by 40%. The pharmacognostic evaluation of this medicinal drug will be supportive in the detection of adulteration and quality control. Due to the herbicidal potential, it can be used in the agricultural industry. For future studies, it is suggested to understand the mode of action of its active compounds through...
Tehreem Zahra1, Muhammad Irfan1, Muhammad Nadeem2, Misbah Ghazanfar1, Qurratulain Ahmad3, Shaukat Ali4, Farzana Siddique5, Zarina Yasmeen6, Marcelo Franco7
...variables like substrate concentration, peptone and KH2PO4 were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity was obtained after 96 hours of incubation with optimum conditions of media containing 5% substrate concentration, 0.05% peptone and KH2PO concentration o...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
...degradation at different concentrations and durations. However, inhibition of autophagy by 3-MA rescued the survival of Hela cells. This study demonstrated that the potential effect of spermidine on decreasing the survival of Hela cells is attributable to autophagy induction.
...
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
...ed that the average eDNA concentration of Sepiella japonica extracted by three kits were (4.62 ± 0.72) × 105, (3.83 ± 0.56) × 105, and (2.35 ± 0.27) × 105 copies/300 ml, respectively. The eDNA concentrations by AxyPrep DNA Gel Extraction Kit were about 83% of that extracted by DNeasy Blood and Tissue Kit. However, extracting cost of an eDNA sample...

Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3

...evels of plasma FSH. The concentrations of testosterone and LH were found to be significantly reduced in kisspeptin treated groups of 1µg dose (p<0.01) and 1ηg dose (p<0.05) whereas the dose of 10ρg had shown no significant change. Kisspeptin doses of 1ηg and 1µg lead to significant reduction in quantity of elongated spermatids and daily sperm production (p<0.05; p<0.001), step 7 spermatids (p<0.05; p<0.001), type A sp...
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...plant species, botanical concentration and exposure time. The extracts of Azadirachta indica (neem) and Nerium indicum (oleander) appeared to be most effective against the termites with minimum LC50 (6.35 and 10.38%, respectively) and LT50 (12.11 and 17.49 h, respectively) values, followed by the extract of Gardenia jasminoides (gardenia). While the extracts of N. indicum (oleander) and Dodonaea viscosa ...
Mehwish Saleem Khan
Evaluation of Changes in Zinc Levels in Patients Suffering from Cancer
... evaluate the serum zinc concentration. The sample zinc level is estimated by using the spectrophotometer in laboratory. The level of zinc in cancer patients was observed lower than the optimum value. Male have much lower concentration as compare to females, therefore male are more susceptible to cancer than female. In the nutshell Zinc is an effective anti-inflammatory and antioxidant agent, including as a natural cancer tr...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...er biomass and glycoside concentrations through greater system productivity. Hitherto, there was no significant study about the effect of far-red LED induction to improve the steviol glycoside content of Stevia plants, particularly at the molecular level. This research was therefore performed in the TIS RITA® bioreactor system to evaluate the effect of far-red LED induction towards biomass growth, multigene expression related to steviol glycosides, and its...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3

...ccp Pakistan strain. The concentration for each drug was measured distantly depending on high concentration available in the market and was then used in various concentrations ranges from higher to lower i.e. moxifloxacin 80-0.156 µg/ml, levofloxacin 250-0.488 µg/ml, ciprofloxacin and enrofloxacin 50-0.097 µg/ml. The resultant effective antimicrobial by minimum inh...

Zubia Rahim1, Gulnaz Parveen1*, Salma Gul2 and Khushnood-ur-Rehman3

Ameliorating Effects of Salt Stress (KCl, NaCl) on Growth and Germination Parameters of Pearl Millet (Pennisetum americanum)
...of NaCl + KCl in various concentrations on the growth and germination of Pearl millet (Pennisetum americanum), locally named as Bajra. Five seeds sown in petri dishes to observe the radical, plumule length and germination percentage after treatments to 100, 150, 200 and 250mM concentration of above mentioned salts. Control petri dishes/ pots were irrigated with distilled water. The experiment was extended to study growth (nu...
Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3
Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation
...e shoots (7.33) when the concentration of BAP and IAA was 2.5 and 1 mg L-1, respectively. Alike, Wiallium-8818 hybrid produced the longest shoot (12.20 cm) with more leaves (6.23) at 1 mg L-1 of each BAP and IAA. But Pisang variety produced maximum fresh weight (15.45 g) with a greater number of roots at 5 + 1 mg L-1 of BAP + IAA, respectively.

...

Muhammad Yasir*, Mansoor ul Hasan, Muhammad Sagheer, Amer Rasul, Rameesha Amjad Ali and Habib ur Rehman

Evaluation of Spinosad Applied to Grain Commodities for the Control of Stored Product Insect Pests
...maize, rice and oats) at concentrations of 0.25, 0.50 and 1 mg Kg-1 under laboratory conditions maintained at 28 ± 2oC, 65 ± 5% RH and continuous darkness. Seven bioassays were conducted by releasing the insects on treated commodities after different post treatment periods (0,1, 2, 3, 4, 5 and 6 months). The mortality of three insect species was recorded at tested concentrations in all the treated commodities a...

Muhammad Jahanzaib1*, Nazakat Nawaz1, Muhammad Arshad1, Shehzadi Saima4, Muhammad Suhaib2, Muzammil Husain3, Haris Khurshid1 and Shahid Ali Khan1

Effect of Temporal Application of Gypsum on Mineral Uptake and Economically Important Morphometric Traits in Groundnut (Arachis hypogea L) under Rain-fed Conditions
... and minerals (Ca, K, P) concentration in root and shoot have been examined. A newly released Pothowar groundnut variety (Variety name) was grown in 3 replications with 5 m row length using a randomized complete block design. Five different treatments were applied in two split doses i.e. T1 (0 %:100 %), T2 (25 %:75 %), T3 (50 %:50 %), T4 (75 %:25 %), and T5 (100 %:0 %) at the time of sowing and pegging compared with Check (T0). Results showed significant diffe...

Sajid Ali1, Abdul Basit1,3*, Abdul Mateen Khattak1, Syed Tanveer Shah1, Izhar Ullah2, Noor Alam Khan3, Imran Ahmad1, Kamran Rauf1, Salman Khan1, Irfan Ullah1 and Intizar Ahmad1

Managing the Growth and Flower Production of Zinnia (Zinnia elegans) through Benzyle Amino Purine (BAP) Application and Pinching
...inins and decrease auxin concentrations flourishing the axillary buds. A research study on the growth and production of zinnia flowers through application of BAP (25, 50, 75 and 100 mg L-1) and pinching (2, 4 and 6 leaf stages), was carried out at The University of Agriculture Peshawar, Pakistan. Research analysis showed that zinnia plants treated with 100mg.L-1 and pinched at 6 leaves stage recorded maximum days to flowering, flower persistency, branches, lea...

Noor Ayaz1*, Faiza Aman1, Sidra Saleem1*, Maqbool Rehman1 and Muhammad Fahim2

Olive Cuttings as Affected by Different Concentrations of Indole Butyric Acid
... the effect of different concentrations of indole butyric acid (IBA) on olive cuttings, a greenhouse study was conducted, at Agricultural Research Institute, Tarnab during the growing season 2016. Completely randomized design (CRD) was used with four treatments that were replicated three times. The IBA concentrations used were (T1: control, T2: 1000 mgL-1, T3: 2000 mgL-1, T4: 3000 mgL-1). Data were recorded on days to 50% sp...
Muhammad Shafiq1,2,*,Rajwali Khan1, Ilyas Ali3, Sadeeq Ur Rahman4, Saif Ullah3Shah Jan Mohammad2, Mohammad Jan2 and Jinhu Huang2
... and serum total protein concentration (b) colostrum immunoglobulin level and (c) their respective calves’ serum immunoglobulin and serum total protein concentration. Three breeds of cattle were observed: Jersey, Holstein Friesian (HF) and local Pakistani cow breed Achai. To assess serum IgG, sodium sulphite precipitation technique was used while IgG in colostrum were determined using digital Brix refractometer ...

Ambreen Akhtar Saddozai1, Amer Mumtaz2, Naseem Rauf1, Saeeda Raza2, Nouman Rashid Siddiqui2, Muhammad Naeem Safdar2, Sahar Shibli2, Muhammad Suhail Ibrahim3*, Muhammad Akhtar4 and Muhammad Saad Rehan5

Preparation and Quality Evaluation of Soymilk Carrot Blend
... milk at three different concentrations levels 2%, 4% and 6% and packed in sterilized bottles. The product was kept at two different temperatures (ambient and refrigerated temperature) for 14 days to assess its shelf life. During the storage period the samples were analyzed for different chemical parameters total soluble solids, pH, total carotenoids, protein contents and antimicrobial analysis. On the basis of quality attributes it was observed that plain and...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Sher Muhammad3, Usman Saleem4, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz5 and Aamer Sattar2

Impact of NaCl Toxicity on Yield and Yield Components of Rice (Oryza sativa) Grown in Aridisol
...s more pronounced as the concentration of salt increased in the treatments.

...
Shikang Deng1,2, Yan Jin2, Jing Xu2, Xiufang Zhu2, Pinghai Hu2, Jiao Li2, Li Zhang2 and Jianzhong Tang2,*
...sts were given different concentrations of 5-FU (0, 0.001, 0.01, and 0.1 mmol/L). After 48 h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed ...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...s no difference in semen concentration, sperm motility and useable spermatozoa/ejaculate before 32 wk of age (p>0.05). Feed quantity was correlated positively with sperm concentration and sperm motility. At 50 wk of age, higher sperm motility was observed in males fed with 135.5and 125.5 g/d diet than males fed with 115.5 g/d diet (p<0.05). While feed quantity was correlated positively with semen volume, sperm

Rahat Afza, Muhammad Asam Riaz*, Muhammad Afzal and Muhammad Zeeshan Majeed

Adverse Effect of Sublethal Concentrations of Insecticides on the Biological Parameters and Functional Response of Predatory Beetle Coccinella septempunctata (Coleoptera: Coccinellidae) of Brassica Aphid
...cides at their sublethal concentrations was assessed on the biological parameters and functional response of Coccinella septempunctata. All insecticides significantly reduced the fecundity, TPOP (total pre-oviposition period), larval weight and increased the life span of C. septempunctata relative to control. Thiamethoxam and lambda-cyhalothrin non-significantly reduced the coccinellids larval weight. Type II response was shown by larvae of C. septempunctata i...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz3, Abid Niaz2, Khurshid Ahmad Mufti4 and Muhammad Arif2

Deleterious Impact of Sodium Chloride (NaCl) Toxicity on Chemical Properties of Soil and Ionic Composition of Rice
...late SAR values of soil, concentration of Ca2+, Mg2+ and Na+ were also determined from all soil samples. It was observed from the results that addition of NaCl proved toxic for different chemical properties of soil as values of pH (7.23 to 8.44), EC (2.96 to 13.54) and SAR (2.45 to 11.57) were impaired negatively and in systematic manner with the increase of NaCl concentration in soil and in all treatments when compared with...

Muhammad Umair Hassan1, Muhammad Aamer1, Muhammad Nawaz2, Abdul Rehman3, Talha Aslam3, Ubaid Afzal4, Bilal Ahmad Shahzad3, Muhammad Ahsin Ayub5, Faryal Ahmed1, Ma Qiaoying1, Su Qitao1 and Huang Guoqin1*

Agronomic Bio-Fortification of Wheat to Combat Zinc Deficiency in Developing Countries
...tant ones to increase Zn concentration in wheat grains up to desirable levels. The genetic techniques are costly, and sustainable, however, they are long term and requires large breeding activities and resources. Conversely, agronomic techniques appear to be short term, quick and economic solution to increase the Zn contents to meet human needs. The application of Zn as foliar sprays, seed priming, soil application and soil+foliar application effectively enhan...

Muhammad Farooq1*, Elham Azadfar2, Zohre Bahrami3, Mahniya Sharifi3, A. Shakoor4, Shabir Ahmed5, Iftikhar Ahmed Solangi1, Muhammad Noman Khan6, Memona Siddique7, Naila Ilyas8*, Muhammad Bakhtiar9, Mohamed El-Fatieh Ismael1 and Wang Yunyang1

Optimization of Drying Process of Rambutan by Combined Method of Osmosis Ultrasound and Complementary Drying of Hot Air
...is-ultrasound with 40.5% concentration in Sekanjabin solution then dried in an oven at a temperature of 70°C. The characteristics of solid gain, water and weight loss, shrinkage and rehydration were evaluated. The obtained results showed that by increasing the concentration of weight and water loss, solid gain increased. Accordingly, in a higher concentrated osmotic solution, the osmotic pressure, which is a key factor i...
Muhammad Farooq1*, M. Javed Iqbal2, Rizwan Shukat2, Qayyum Shehzad3, Shabir Ahmed4, Elham Azadfar5, Abdul Saboor6, Ibrar Ahmad7, Iftikhar Ahmed Solangi1, Kaleem Kakar8, Shoaib Khan9 and Wang Yunyang1
Development of Onion Powder by using Egg Albumin as a Foaming Agent by Foam-Mat Drying Process
...s treated with different concentration of egg albumin (0%, 4%, 8% and 12%) as foaming agent and Carboxyl methylcellulose (0.5%) as foam stabilizer and these were dried in hot air tray drier at different temperatures (55°C, 65°C and 75°C) with 3mm sheet thickness of onion foams. Effect of different concentration of foaming agent and drying temperature was studied on moisture and drying rate of onion paste. Increas...
Muhammad Umar1, Muhammad Arshad2, Mubashar Hussain1*, Moazama Batool3 and Muhammad Faheem Malik1
...er than safe limits. The concentration of these metals showed variation in different months of the year. An extensive monitoring of the lake and catchment area is suggested in order to address the threats posed by heavy metals.
...
Xiaopeng Tang1,*, Lei Chen1, Kangning Xiong1, Dun Deng2 and Peng Peng2,*
... treated with increasing concentration of DDGS. Compared to mock-treated group, the average daily gain (ADG), average daily feed intake (ADFI) and feed to meat ratio (F:G) were measured. It was revealed that the addition of DDGS up to 24% in the diet remained ineffective for ADFI, ADG and F:G. However, there a tendency of decrease in ADG was observed when 32% DDGS was added in the feed. Taken together, our results demonstrate that the increase of DDGS in feed ...
Faiza Altaf1, Shamim Gul1,2*, Tasawar Ali Chandio3, Gul Bano Rehman1, Attiq-ur-Rehman Kakar4, Sami Ullah4, Naqeebullah Khan4, Umbreen Shaheen5, Muhammad Naeem Shahwani6, Muhammad Ajmal7 and Misbah Manzoor8
 
 
...manure on the growth and concentration of heavy metals in edible tissues of tomato and lettuce. These biochars were separately mixed with poultry manure as 1:1 ratio on air-dry weight bases. Soil was contaminated with chromite mine tailing debris at 2% amendment rate (980 g soil + 20 g debris). Biochar-poultry manure mixtures were applied in soil at 10% and 20% amendment rates (respectively, 900 g soil + 100 g mixture and 800 g soil and 200 g mixture of poultr...
Javeria Afzal, Muhammad Abid, Faisal Hussain* and Alia Abbas
...m stem extract at 2% concentration. The minimum reduction (0.33%) was observed in the leaf extract of A. viridis at 10% concentration after 48 hours of exposure time. In larval mortality test, maximum larval mortality (33%) was recorded in C. album leaf extract at 10% concentration while minimum larval mortality (0.66%) was noted in Solanum nigrum at 2%
Naveed Akhtar1*, Muhammad Fiaz Khan1*, Sadia Tabassum1
Munawar Saleem Ahmad2 and Khan Dil Badshah3
...ibes effect of different concentrations of cypermethrin on hematological parameters, biochemical components of blood, histopathological changes in different organs and DNA damage in Chirruh snow trout Schizothorax esocinus. WBCs, RBCs, hemoglobin, hematocrit, MCV, MCH, MCHC and platelets were significantly decreased in all treatedgroups compared to control. Monocytes showed significant increase in all experimental groups. Lymphocytes counts significantl...
Muhammad Younis1, Muhammad Irfan-ur-Rehman Khan1*, Mustansar Abbas1Ali Murtaza1, Imran Mohsin2, Muhammad Shahzad3 and Muhammad Zahid Tahir1
...ent and steroid hormones concentrations during estrous cycle of Lohi sheep. Ovaries of nine Lohi ewes were monitored for two consecutive estrous cycles using B-mode trans-rectal ultrasonography during the breeding season (Sep-Nov 2018). Plasma progesterone and estradiol-17β concentrations were determined throughout the cycle using radioimmunoassay. The average length of the estrous cycle in Lohi sheep was 17.0±0....
Kamal Al-Samawi1, Mohamed Al-Hassan2, Hussein Migdadi3,4*, Megahed Ammar5 and Salem Alghamdi3
... serum progesterone (P4) concentrations were assayed using an ELISA kit. Pregnancy detection was performed by US on 23, 35, and 60 days post NM. Serum P4 concentration was significantly higher in pregnant than non-pregnant goats at 15, 23, 35, and 60 days post NM. Relative expression of mRNA of ISG15 and ISG17 was significantly higher in pregnant goats at 7, 15, and 35 days post NM. ISG15 and ISG1...
Muhammad Naeem1,2, Nasir Rajput1,2, Sher Ali3, Asmatullah Kaka2, Dildar Hussain Kalhoro2, Mehvish Rajput2 and Tian Wang1,*
...H than other groups. The concentration of acetate, butyrate and iso-butyrate were almost similar in TRS-II, AH and CWR; valeric and iso-valericwere lower (P<0.05) in CWR and TRS-I than AH and TRS-II, while the propionate and total VFA were higher in AH but similar in CWR and treated rice straw. The in vitro degradibility of DM, OM and NDF were significantly higher for AH and TRS-II while, ADF was higher for AH. Steam treated straws show...

Saleem Maseeh Bhatti1*, Aslam Khan Gadehi1, Inayatullah Rajpar1, Muhammad Nawaz Kandhro2, Mukesh Kumar Soothar1 and Zohaib Ur Rehman Bughio1

 

 

...±0.27 grams). The concentration of Na+ increased up to 3.4 times and Cl- concentration enhanced up to 2.7 times in leaves of spinach genotypes while no profound effects on K+ concentration in leaves was observed. English spinach accumulated significantly more Na+ and Cl-, and less K+ than Sindhi spinach. In soil, the ...
Iftikhar Hussain1, Sardar Azhar Mehmood1, Muzafar Shah2*, Mohammad Salim3, Shabir Ahmed1, Sana Ullah1,Sidra Batool4, Nadia Saeed1 and Khalid Usman1
... sequences showed higher concentration of A+T as compared to G+C. The A+T contents were 53.1% and C+G were 46.8%. In phylogenetic tree the sequences of C. sphinx clustered together with 100% bootstrap support. The relationship between C. sphinx and C. horsefield was supported by 83% bootstrap value. The relationship between C. titthaecheilus and C. horsefield was supported by 98% bootstrap v...
Hafiz Hammad1, Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Abid Niaz4 and Muhammad Arif4
... P accumulation, plant P concentration, P uptake efficiency and PE ratio were recorded using standard procedures and obtained data was analyzed statistically with a statistical software Statistix 8.1 analysis of variance technique and significant of treatments was tested using LSD test at probability level of 5%. The maximum P accumulation (178.37 mg P pot-1), P uptake efficiency (1.90) and PE (phosphorus efficiency) ratio(747.15 g DM (g plant-...
Hafiz Tassawar Abbas1*, Tamoor Khan1, Ghulam Khaliq2, Muhammad Aqeel Sarwar3, Muhammad Rashid4, Intazar Ali5, Muhammad Abuzar Jaffar2, Ghulam Ali Bugti6 and Muhammad Waseem4
 

...and 70 ppm difference in concentration of N, P, K, Ca, Mg, Na, Zn, Fe and Cu in “Thall-2006” variety respectively. Resistant germplasm contained greater concentrations of these minerals related to susceptible lines/varieties. Increased mineral contents in resistant plants build up the physiological and biochemical methods of the host plants which help to prevent the extent of pathogen. 

...
BamideleAkinsanya1, Isaac O Ayanda2,*, Benson Onwusa1 and Joseph K Saliu1
...site. Total BTEX and PAH concentrations in the parasite was 47.45±7.68 and 135.12±2.547, respectively. There are ecotoxic concerns in the surroundings of the lagoon and human activities that aid it should be checked.
...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2,  Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...ded with three different concentration (5, 7.5 and10 mmol) of L-cysteine diluents and followed by other three concentrations (1.2, 2.4 and 4.8 mmol) of vitamin E diluents and, the last aliquot of semen was extended with control (no additives) to semen diluent. The spermatozoa freeze-thawed quality were assessed and found that total motility, progressive motility, membrane functionality, acrosomal integrity, and viability per...

Masood Sadiq Butt1, Sadia Aslam1,2, Rizwan Shukat1,*, Syed Qamar Abbas1, Muhammad Issa Khan1, Shadab Shaukat3 and Muhammad Shahid4

...ydrolysate contains high concentration of protein (72.55%) and amino acid (99.71 g/100g) of which 31.2% are essential amino acids. Chemical score of protein hydrolysate was closer to FAO/WHO reference protein. Tryptophan was found most limiting amino acid in fish protein hydrolysate. The nutritional value of fish protein hydrolysate was suitable to suggest it as potential food ingredient in functional foods.
...

Memoona Tehseen1, Sajila Hina2*, Alim-un-Nisa2 and Naseem Zahra2

...oxidant. Three different concentrations of spinach extract, SFO-800, SFO-1600 and SFO-2400 ppm were supplemented to sunflower oil at 25oC and 60oC along with standard synthetic antioxidants butylated hydroxianisole (BHA), butylated hydroxytoluene (BHT). The stabilization of sunflower oil with spinach extract as natural antioxidant was monitored by evaluating oxidation in terms of peroxide value, free fatty acid value, iodine value, conjugated dienes, conjugate...

M. Javed Iqbal1, Rizwan Shukat1, Muhammad Farooq2*, Iftikhar Ahmed Solangi2, Naila Ilyas3, Rahman Ullah4, A. Shakoor5, Muhammad Bakhtiar6 and Faraz Ahmed7 

...s treated with different concentration of soy protein (0%, 4%, 8% and 12%) as foaming agent and Carboxyl methylcellulose (0.5%) as foam stabilizer and these were dried in hot air tray drier at different temperatures (55°C, 65°C and 75°C) with 3mm sheet thickness of onion foams. Effect of different concentration of foaming agent and drying temperature was studied on moisture loss drying rate of onion paste. Increa...

Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*

... (PCV), hemoglobin (Hgb) concentration, red blood cells (RBCs) count, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) platelets count, mean platelet volume (MPV), white blood cells (WBCs) count, monocytes count, lymphocytes count and granulocytes count. Serum biochemistry profiles (total protein, Albumin and creatinine) of treatment group animals were also ch...
Khaliq Dad1, Muhammad Nawaz2*, Rumsha Hassan2, Kainat Javed2, Asma Shaheen2, Fengliang Zhao3, Muhammad Imran4, Syed Tansir Hussain Shah4, Muhammad Faraz Anwar5 and Muhammad Aurangzaib6
 
... of Cd by plants. As the concentration of Cd increased to high i.e. 15 mg/kg of soil, the limiting capacity of biochar was also seen less effective.

...

Saleem Maseeh Bhatti1*, Muhammad Aslam Panhwar1, Zohaib ur Rehman Bughio1, Muhammad Saleem Sarki1, Allah Wadhayo Gandahi1, and Niaz Ahmed Wahocho2

...on growth, yield, and Zn concentration in strawberry (Fragaria x ananassa cv. Silder) at Nasarpur, Sindh, Pakistan during 2015-16. The foliar rates comprised of 33, 66, and 99 mg Zn L-1 (equivalent to 100, 200 and 300 mg ZnSO4 L-1) and distilled water (control treatment) and sprayed to strawberry plants before flower initiation. A continual increase in number of leaves, number and weight of berries, fresh weight of strawberry plants, and Zn

Ch. Muhammad Rafiq1, Qasim Raza1*, Awais Riaz1, Misbah Hanif2, Wajiha Saeed2, Shawaiz Iqbal1, Tahir Hussain Awan1, Syed Sultan Ali1 and Muhammad Sabar1

...five salicylic acid (SA) concentrations (0, 75, 150, 225 and 300 ppm) on polyethylene glycol (PEG) induced drought stress conditions (0, -0.2, -0.4, -0.6 and -0.8 MPa). Highly significant (p < 0.01) effects of drought, SA and their interactions were observed on seed germination. Interestingly, varying SA concentrations imparted more pronounced effects under higher osmotic stress levels. Day-wise germination data indicated...

Fazal Wahid1, Arif Ullah Shah1, Muhammad Rahim2*, Fazal Dad1, Nadir Khan1, Saqib Ullah1, Yasir Ali1 and Sayed Ashfaq Ali Shah3

... Pb < Ni < Fe. The concentration of Fe in all soil sample was found in the maximum ranges while those of Co in the minimum ranges. 

...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2
Mark Wen Han Hiew1 and Lawan Adamu1
...he addition of different concentrations of antioxidants to evaluate oxidative stress and fertility of frozen semen in horses. Seven stallion were used for semen collection in this study. A total of twenty ejaculates was collected from three healthy stallions twice a week for the experiments 1 and 2. All ejaculates were divided into two parts of ten ejaculates each, ten ejaculates for cysteine in experiment 1 and the other ten for ascorbic acid groups in experi...
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
...osed to three sub-lethal concentrations (37.5µg/g; 18.7µg/g; 9.37µg/g B.W) of aluminum, with and without onion extract. Control and antidote groups were treated with distilled water and onion equous extract, respectively. Doses were administered orally by gavage for 30 consecutive days, once a day and mice were euthanized on the 31st day. Blood was collected in serum separating tubes through intracardiac puncture for biochemical an...

Muhammad Junaid*, Musharaf Ahmad and Saifullah

...ded to the soil at three concentrations i.e. C1= 1%, C2=5%, and C3=10%, to amend topsoil. Results revealed that increase in amendment’s concentrations to 10% decreased the chance of plant survival to 68.06% while maximum (89.72%) number of plants survived for 1% amendments concentration. Significantly high number (87.22) of plants survived for green manure as compared to FYM with 78....
Saba Afzal1, Sohail Raza1,*, Masood Rabbani1, Sehrish Firyal2, Imran Altaf1 and Zahra Naeem1
...showed that 2.5 µM concentration of the ivermectin is non-toxic to the Vero cells. At this concentration, PPRV titre was significantly reduced (p < 0.001) by two log to 103.0 TCID50/0.1 mL as compared to virus control 105.5 TCID50/0.1 mL. Moreover, the ivermectin exposure after the viral attachment and entry steps able to reduce virus titre more as compare to viral ...

Jabeen Farheen1,2*, Simeen Mansoor1 and Maria Abid1

...01%, 0.01%, 0.1%, and 1% concentration of FCA for 120 hours for macroscopic and 36 h for microscopic evaluation. The onion root tips morpho-physiology was severely affected as the concentration of FCAs increased. The macroscopic analysis manifested that allura red had 95% broken-ended extremely thin and transparent root tips at 1% concentration. In the microscopic analysis, FCAs induced ei...

Umar Farooq1, Muhammad Akmal1, Qadeer Ahmad2, Zahid Akram2, Adnan Arshad3, Huma Qamar4*, Hafeez Ullah5, Muhammad Zubair4 and Muhammad Rizwan Khurshid4

...il weight gave higher Fe concentration (3.32 mgkg-1) in grains. The lowest concentration of Fe found in control treatment (2.15 mgkg-1). The results revealed that various Fe-enriched organic amendments increased crop growth and yield related attributes like number of pods per plant, plant height; seed yield per plant and improved the quality by increasing iron concentration in grains. The ...

Saima Naz1*, Ahmad Manan Mustafa Chatha2, Saba Saeed1

...y (96-hr LC50 and lethal concentrations) for two fish species viz. Labeo rohita and Cirrhinus mrigala was determined in this study. During metal stress trials both species were kept at constant temperature (32°C), pH (7) and hardness (250 mg L-1) of water. Physico-chemical parameters of test medium were monitored regularly. The observed mean LC50 and lethal concentrations for Cirrhinus mrigala were 55.85 ± 2.84 an...
Mohammed  Akinlabi Rufai1*, Abideen Akinkunmi Wahab2, Kamil Ayo Fasasi1, Quadri Olusegun Adeshina1 and Mary Tolulope Awotidebe1
...tion. The five different concentrations of H. bacteriophora were made in distilled water (µg/l); one sample not treated with nematode was served as control. Biocidal efficacy was achieved by collecting soil samples, sterilized and kept into experimental plastic plates labelled as A, B, C, D, and E. This was further divided by weight into five equal portions labelled as A1-A5, B1-B5, C1-C5, D1-D5, and E1-E5, respectively. The observation was done after ev...
Yawang Sun, Yongjiang Wu, Jingbo Chen, Zili Wang, Juncai Chen, You Yang and Guozhong Dong*
...vely correlated with LPS concentrations. The production of reactive oxygen species increased in a dose-depend manner. Cellular concentrations of oxidative damage markers were positively correlated with applied LPS concentrations. Total antioxidant capability and the activity of superoxide dismutase (SOD) increased with increasing LPS concentrations. Gene...

Muhammad Nawaz1*, Muhammad Dawood1, Kainat Javaid1, Muhammad Imran2, Fengliang Zhao3, Shahzadi Saima4 and Syed Tansir Hussain Shah2

... the pots. Six different concentrations of fluoride (3mg/L to 15mg/L) along with water as control were used periodically. At both harvests, maximum growth and physiological functions were recorded at 0mg/L treatment while, reduction was recorded at 15mg/L.

...

Qaiser Shakeel1*, Rabia Tahir Bajwa1­, Yasir Iftikhar2, Mustansar Mubeen2, Muhammad Luqman3, Waqas Ashraf1 and Ifrah Rashid4

...ringa with 82%, the best concentration is 100mg/mL. Besides, Ridomil gold showed the most effective control against ginger soft rot with 84% of mycelial inhibition growth among all the tested fungicides. And found that protective treatment had a best effect than curative treatment. These results revealed that plants are rich source of metabolites with a wide range of antifungal activity against ginger soft rot. Crude extracts of these plants have potential to ...

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sher Muhammad3, Muhammad Latif4, Ayesha Zafar1, Imran Shehzad1, Sarfraz Hussain5, Aneela Riaz6 and Abid Niaz2

...%) and potassium (2.85%) concentration in shoots of maize plants. The same trend of improvement was noted for maximum nitrogen (3.16%), phosphorus (0.92%) and potassium (2.88%) concentration in roots of maize plants.

...

Taqi Raza1*, Kashif Nazir Qureshi2, Shakeel Imran2, Neal Samuel Eash3 and Imma Bortone4

...omparison of heavy metal concentration (mg/L) showed the concentration of Cd, Cr and Ni were within the safe limits; however, Fe and Pb were higher than the safe limits in all industrial effluents. The highest/unsafe concentration of metal Fe was recorded 4.093, 2.979 and 2.959 mg/L in the effluents of chemical, textile and ghee mill respectively. While the highest/unsafe

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sarfraz Hussain3, Ayesha Zafar1, Imran Shehzad1 and Aneela Riaz4

...nd potassium (213.0 ppm) concentration in soil was also increased in this treatment.

...

Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Abdul Moeez1, Muhammad Zeeshan Manzoor1 and Aneela Riaz3

... conditions, sodium (Na) concentration (5.63 mg/g), potassium (K) concentration (9.20 mg/g), Na/K ratio (1.60 mg/g), relative water contents (56.49%) and total chlorophyll contents (2.74 mg/g) were observed when maximum concentration of Si was applied i.e. 100 µg Si/g and least was recorded when no Si was applied. Similarly, under saline soil conditions, maximum sodium

Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Abdul Moeez1, Humaira Ramzan1, Mudassar Hafeez1, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Sher Muhammad4 and Muhammad Latif4

...environment when maximum concentration of Si was applied i.e. 100 µg Si/g and least values were obtained when no Si was applied. Thus, application of Si as higher concentration (100 µg Si/g soil) proved superior to mitigate harmful effects of salts on rice plants.

...

Habiba ur Rehman, Muhammad Usman Ghazanfar and Waqas Raza

...ate the most appropriate concentration of two different fungicides against the charcoal rot of sunflower caused by Macrophomin phaseolina under greenhouse conditions. The treatments viz. Success, Nativo and Control with the concentrations of 2, 4 and 6mM were used with three replications under completely randomized design in greenhouse conditions. Nativo had given the most significant results in all studied traits as compare...

Muhammad Irfan Ullah1*, Sana Majeed1, Muhammad Arshad1, Nimra Altaf1, Muhammad Luqman2, Asad Abdullah1 and Muhammad Afzal3

...ophorus extract at 50 mg concentration was comparable to the alone application of emamectin benzoate in terms of reduced digestibility and survival of S. litura. Overall, 42.7% relative consumption rate (RCR), 74.3% efficiency of conversion of ingested food (ECI), 72.3% efficiency of conversion of digested food (ECD), 87.5% relative growth rate (RGR), 11.04% approximate digestibility (AD), and 47.6% assimilation rate (AR) were reduced after application of a mi...

Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*

... 2016. The chlorophyll-a concentration was determined by using the acetone extraction technique. Ponds water sample (50 liters) was filtered through plankton net of 20 µm pore size and preserved in 4% formaldehyde for the analysis of phytoplankton. Subsequently, the phytoplankton were counted under microscope. Result showed that water quality parameters of all fish farms were suitable for farming purpose excluding Shamimul Hassan Farm, Fish Farm Green Co...

Misbah Ali1, Safdar Ali1, Muhammad Ahmad Zeshan2*, Rana Binyamin3, Nadeem Ahmed4, Muhammad Usman Ghani5 and Awais Ahmed Khan2

... vitro trials with three concentrations (150 ppm, 200 ppm, and 250 ppm). The most effective concentration of fungicides from laboratory experiment was used in field conditions against red leaf spot of sorghum. Results showed that Cabrio Top was the most effective fungicide by showing 88% fungal inhibition while Native also exhibited good results by showing 78.1% fungal inhibition. The remaining 2 fungicides Melody Duo and Al...

Weenghar Ali Chandio1, Tajnees Pirzada1*, Abdul Majid2 and Farzana Rashid3 

...gated by applying varied concentrations of HA (0, 5, 10, 15, and 20ppm).UV-Visible spectroscopy confirmed the presence of HA in soil sample. XRD demonstrated that HA extracted had semi-crystalline structure with the average crystal size of 39.48 x 109nm. FTIR showed the presence of Oxygen-containing functional groups (aromatic) such as ether bonds, ester bonds, carboxyl group, phenolic hydroxyl group as well as aliphatic hydrocarbon including methyl group, met...

Tahseen Ullah* and Noorul Amin

... of stem cutting and IBA concentration have significantly affected (at P≤0.05) the sprouting %, survival %, sprout weight, dry leaves weight, leaf area, root diameter, chlorophyll content, sprout diameter, fresh root weight, number of branches, shoot length and root length while the days to bud sprouting, fresh leaves weight and number of roots have non-significantly affected by IBA concentration and types of stem cutting...

Saddar Faheem1,2, Hameeda Kalhoro1, Naeem Tariq Narejo1*, Muhammad Hanif Chandio3, Memon Samina4, Shahnaz Rashid5 and Ghulam Abbas5*

...100 ml to 7.06 g/100 ml) concentration were significantly higher in male fish during summer and winter than that of female. In female fish, erythrocyte sedimentation rate (ESR) in summer and winter season was found higher (7.3 mm/h to 2.56 mm/h). Thrombocytes, neutrophils, lymphocytes, eosinophil and monocytes did not show any significant difference in their counts of male and female fish during summer and winter season. Important serum constituents like prote...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
.... The minimum inhibitory concentration of ESBL producing isolates was determined by the broth dilution method. Phenotypically confirmed ESBL producing strains were further subjected to molecular characterization for the presence of ESBL and carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL positive E. coli were recovered and among these 156/213 (73.2%), 42/63 (66.6%) and 21/27 (77.7%) were from poultry, environmental water, and urine sam...
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
... persica was used in two concentrations. MDBK cells grown on
Minimum Essential Medium (MEM) were used for virus propagation and titration. The
effects of two concentrations of Salvadora persica on viral growth in MDBK cells as
well as cytolytic activity of BHV-1 were evaluated at different times pretreatment
,simultaneouslly and after adsorption. The results demonstrated that t...
El-Dougdoug K.A.1, Sofy A.R.2, Rehab A. Dawoud3 and Korkar H.M.4
... containing high sucrose concentration at low temperature. Shoot tips and
subculture 1st of banana plantlets were preservedsuccessfully for 12 months on
storage MS medium supplemented with 40, 50, 60 and70 gL-1 at 10, 15 and 20ᴼC.
The highest survival percentage 100% with recorded with 40 and 50gm sucrose
concentrations at 10, 15 and 20°C while 70 gL-1at 20°C recorded...
Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1
...d in presence of NaCl at concentration higher
than 15%; sodium benzoate at concentration of 0.5%; potassium sorbate at
concentration of 1.0 % and citric acid at concentration of 1.0% as well as in
presence of 5% of either Sodium hypochlorite or SDS. The phage DNA was
resistant to digestion with PvuI, BamHI,...

D. N. Abd-elshafy1, 2 and M. M. Bahgat2, 3

...s carried out using safe concentrations of the cocktail protease inhibitor MixG and its five components: ABESF, aprotinin, E-64, leupeptin and EDTA. Also quantification of the activities of both intracellular and secreted HepG2 proteases was carried out using specific chromogenic substrate at different ranges of pH values. Results showed that EDTA has intracellular metalloprotease inhibitory effect that enhances rate of HAV replication and thus reduces time ne...

Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4

...ation revealed that, the concentration of virus preparation estimated using spectrophotometric measurements at 260 nm was 3.004 mg/ml. The min, max nm, Amax./Amin., A260/A280 and A280/A260 ratios were 244, 260, 1.276, 1.512 and 0.662, respectively for BtMV. The corresponding figures of the purified virus preparation of Beet necrotic yellow vein virus (BNYVV) were 2.196 mg/ml, and the min, max nm, Amax./Amin., A260/A280 and A280/A260 were 248, 264, 1.606, 1.237...

Hanaa H. A. Gomaa1; Entsar A. A. Nassar2; K. A. El-Dougdoug3

Ahmed K. El-Attar; Samah A. Mokbel; Ali H. Hamed

.../div>
of different concentration levels of kinetin as well as its efficacy on the OYDV
elimination and regeneration of infected onion bulbs were determined.
...

K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2

...rity infection and virus concentration. Expressed protein
was differed in leaves of potato cultivars. Since PVY infection unexpectedly increased in
(Anabel, Sisi, Lady Balfor, Nicola and Execusa) or decrease of total protein in (Hermes and
Spunta). The potato cultivars (healthy and infected) varied in number and density of protein
bands. As well as specific bands with 72% polymorphism, commonly bands were percent...

Samah A. Mokbel, Soad H. Taha, Mahmoud M. Abd-El Hamid, Ali H. Hamed

...ave been evaluated. Four concentrations of lactoferrin 100, 250, 500 and 1000 mg/L were examined either in vitro culture medium or in vivo (greenhouse). The presence of the virus was evaluated by ELISA technique. Results demonstrated that application of 1000 mg/L lactoferrin by spraying or combined with tissue culture proved to be an effective method for PVX-inhibition as compared with other concentrations. Also, results of ...

AdlyAbd-Alla1,2, MahaNada3, Francois Cousserans1, and Max Bergoin1

...periment confirmed virus concentration
response mortality. Injection of a purified virus suspension to third instar larvae of Galleria
melonella and Spodopteralittoralis failed to induce any mortality indicating that the virus is
most likely host specific to N. viridula. This virus is similar to the previously reported NVV-1
virus in N. viridula from South Africa strongly suggesting that NVV-1 is widely distribut...
M. A. Kararah1, Om-Hashem M. El-Banna1, Salwa N. Zein2 and Abd-Elrehiem, A.F.3 
...respectively.The optimum concentrations of IgG and IgG conjugate were 1.0
μg/ml and 1/1000, respectively. The antigen dilution end point was
1:500.The produced antiserum was evaluated by ELISA and DBIA. Electron
microscopy f ultrathin-section of PMV- infected leaf tissue revaled several
morphological changes and aggregated virus particles associated with
pinwheel inclusion bodies. Salicylic acid an...

Arshad Abdulkhalq Yaseen1,2* and Mária Takácsné Hájos1

...pring 2019 and 2020. The concentration of 6% MLE was sprayed as a biostimulant every two weeks from transplanting with the amount of 25 mL plant-1. The results indicated that lettuce vegetation quality (head structure, head weight and root weight) was significantly improved by the use of 6% MLE, where their combination of MLE and plant variety has also improved some of the vegetation quality parameters. However, significantly the morphological quality paramete...
Eman A. Ahmed1, Osama Y. shalaby2, Emad F. Dwidar2, Samah A. Mokbel1 and Ahmed K. El-Attar1
... wall resulted from high concentration of phytoplasma units; necrosis starts in companion cells; severe damage in chloroplasts with abnormal membrane and thylakoid system was absent. The xylem vesicles were characterized by deforming the secondary walls. Three methods were done towards the production of phytoplasma-free tomato plantlets through tissue culture using antibiotic compound (tetracycline hydrochloride in three concentration<...

Manal A. El-Shazly1. M. I .Kobeasy2and Sarah H . Altalhi3

...ction with TSWV at three concentrations levels (500,1000 and 2000 μg/ml and 50,125,250 μg/ml, respectively).Viral infection was reduced by using the highest concentration of Basil essential oil and ethanolic extract reached to 45.99% and 63.82%, respectively. Different treatments with antiviral compounds decreased the content of total soluble sugars, titrable acidity also increased the lycopene and ascorbic acid in tom...

Shahida Sabir1, Anwar Ali Shad1, Tariq Masood1*, Zafar Ali Shah1, Nasiruddin2, Yaseen Ahmad1 and Syed Sartaj Alam3 

...acts at moderate to high concentrations. Methanolic fraction of mushroom proved to be bactericidal with highest % inhibition (65, 36 and 75 %) recorded at 25 mg/L concentration against all the three bacterial strains i.e., E. coli, Staphylococcus aureus and Xanthomonas sp., respectively. Maximum % inhibition (about 50%) was recorded at 25 mg/L concentration against all the three fungal spe...

Dina N. Abd-Elshafy

...to identify non-cyotoxic concentrations of the
prepared extract. Plaque reduction assay was performed to investigate the anti-HAV activity
of the green tea extract according to Tebas (1995), evaluate its effect, to test effect of
applying green tea extract against contaminated drinking water with HAV and study stability
of inhibitory effect of extract on HAV in water.
Results: Green tea extract has a ...
Arslan Mahmood Ahmad1, Muhammad Ameen Jamal1, Abdul Sattar1,  Aamir Ghafoor2, Aqeel Javeed3 and Amjad Riaz1*
...tudy was to optimize the concentration of SrCl2 for artificial activation of mouse oocytes and to evaluate its toxic effect on early embryonic development.Our results indicated that higher percentage of activation of mouse oocyte was achieved with 10mM and 15mM for period of 3 h and 6 h compared to the control group (P<0.05). To examine the toxic effect, 10mM SrCl2 with 3 h exposure showed minimal detrimental effect on embryo developme...

Youssef A. Elgharbawy1, Mervat M. Ali1, Ayman H. El-Deeb2, Mohamed A. Shalaby2

...ast for 18 hours with 1% concentration of BEI at 37°c. The inactivated LSDV tested on MDBK cells and the animals free of antibodies for residual live virus and determination of minimal protective dose (MPD) of prepared inactivated LSDV. The MPD was 1.5 ml of the inactivated virus and the vaccine was prepared by adding equal volume of the adjuvants (Montanide ISA 50 and Montanide ISA 206 ) to the total quantity 3ml of the vaccine. The prepared vaccine teste...
Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...ated with four different concentration of
NaN3and EMS (1.0, 2.0, 3.0 and 4.0 mM) and (0.3%, 0.4%, 0.5%and 0.6%) respectively, then planted
into a tissue culture media, induced plants were checked for resistance to ToRSV by ELISA. ISSR
reaction was used to confirm the DNA variations.
Results: ToRSV was transmitted mechanically into11 plant species and cvs., belonging to 6 families
while 3 plant species...

Tahsin S. Shoala1, Ahmed K. El-Attar2, Ahmed I. Abd El Maksoud3

...to MS media at different concentrations.
Results: Potato plants those treated with Glycyrrhizic acid ammonium salt (2ng, 4ng, and 8
ng/ml MS media) showed that the presence of PLRV at concentration 2ng/ ml MS media
while they were negatively diagnosed for PLRV at the other concentrations. However, Treated
potato plantlets with curcumin showed...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...lumina . DNA
concentration and sequencing had been performed in demands for faster and more accurate methods to
analyze, characterize and qualify the virus as a seed for future vaccine production. Analysis workflow
starts on a high performance laptop device (GENUS), sequence data is generated for bacteria, viruses,
fungi and archaea that present in the sample it was then classified to subspecies and strain level ...

Moazama Batool1*, Sajid Abdullah2, Huma Naz3, Mubashar Hussain4, Sadia Maalik1, Sajida Mushtaq1, Tanveer Ahmed5 and Laiba Shafique6

... marulius amassed higher concentration of both metals as compared to W. attu. However, Cr accumulates at higher concentration in all organs of both fish species as compared to Cd. The amassment order for metals in both species was studied as kidney>liver>gills>skin>fins>bones>muscle.
 
...

Aliya Munir1, Sajid Abdullah1*, Huma Naz2*, Khalid Abbas1, Tanveer Ahmed3 and Zahid Farooq2

... striata exposed to LC50 concentration (1.374µgL-1) of deltamethrin+endosulfan (DM+ES) mixture for 4-day. Sampling was performed after a 24-hr period. Fish were sacrificed and organs were collected to calculate the POx activity. It was observed that the exposure of DM+ES significantly accelerated the POx level in gills, liver and kidney of fish in a time dependent manner.  

...

Anila Kousar, Muhammad Naeem*, Samrah Masud, Abir Ishtiaq, Zara Naeem and Rabia Iqbal

...ngredients, on elemental concentration in Genetically Improved Farmed Tilapia (GIFT) fingerlings, a developed strain of Nile tilapia (Oreochromis niloticus). Feed preparation criteria was based on cost effectiveness and local availability of cheaper plant protein feed ingredients. Ten specimens were randomly selected from each treatment hapa for evaluating the concentrations of selected essential and non-essential elements i...

Ameer Hamza1, Mukkram Ali Tahir1, Noor Us-Sabah1, Ghulam Sarwar1 and Muhammad Luqman2,*

...be useful by keeping low concentration of salts in the root surrounding area. This study assessed the role of leaching in alleviating the negative effect of saline water on soil characteristics. Results of the studies indicated that 6.57% reduction in soil pH (7.6), 46.06% reduction in EC (1.3 dS m-1), and 46.41% reduction in SAR (5.3) while 17.34%, 5.4% and 5.66% increase in soil organic matter, soil available phosphorus (P) and potassium (K) content respecti...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...udy revealed significant concentrations of Zn, Cd, Cr, Pb, Cu. Except Zn and Pb, the other 3 metals were found below the permissible limit set by WHO. Interestingly, the infertile males having maximum age limit of above 47 years and highest marriage ages during the present study showed comparatively higher concentrations of all the heavy metals. Zinc was found with highest means values while cadmium was recorded with lowest ...
Maimoona Imran1,2, Soumble Zulfiqar2, Hafsa Saeed2 and Farah Rauf Shakoori1*
...size: small;">Increasing concentrations of different essential and non-essential metals in the environment have posed a serious threat to all kinds of life. Bioremediation is considered as the best possible solution among the existing ones to reduce this kind of pollution. In the current study, copper resistant Klebsiella pneumoniae strains KW and CC were investigated for their bioremediation potential. Maximum uptake of Cu++ by mid log phase...
Nabila Kousar1, Tanzeela Riaz2, Anum Feroz1, Abdul Rauf Shakoori3 and Farah Rauf Shakoori1*
...ignificantly whereas the concentration of total proteins, glycogen, trehalose and total lipids was found to be decreased in both populations. Depletion of energy reserves is most probably the cause of mortality of the pest.
...

Muhammad Madni Afzal1*, Shahbaz Talib Sahi1, Amer Habib1, Waqas Ashraf2, Muhammad Ahmad Zeshan3, Muhammad Raheel2 and Qaiser Shakeel2

...ppm, 100 ppm and 150 ppm concentrations. Maximum basal rot disease reduction (78.6%) was recorded at 150 ppm concentration of wild cotton (G. thurberi) under in-vitro conditions. Whereas, wild cotton also gave the highest disease reduction under greenhouse (62.93%) and field conditions (52.56%) as compared with other two plant extracts and control. It is concluded that aqueous extract of wild cotton gave the best control of ...

Ghulam Muhiyuddin Kaloi1* and Mehrunisa Memon

...rial combination of five concentrations of wastewater (0, 5, 10, 15 and 20%) and four rates of inorganic fertilizers (0, 1/3rd, 2/3rd and full) besides additional control (full recommended NPK). A total of 12 irrigations of wastewater were applied after two months of planting up to harvest of sugarcane (CPF-237). Application of 10% wastewater did not show any adverse effect on sugarcane quality and soil health rather improved juice quality and soil fertility. ...

Gulzar Ullah* and Gohar Ayub

...ent (75.34%), Putrescine concentration (193.95 nmol/g), spermidine concentration (154.59 nmol/g), spermine concentration (36.51 nmol/g), yield (20.14 t ha-1), early flowering (26.99 days) and lowest electrolyte leakage (48.87%) was observed in plants treated with 20 hours heat pretreatment duration as compared to other heat pretreatment durations and control. Concerning tomato varieties, n...

Sajid Abdullah1*, Huma Naz2*, Zunera Abbas1, Uzma Nazir1, Mehrunisa Basharat1, Tanveer Ahmed3, Adnan Ahmad Qazi2

...ere exposed to different concentrations (1/3rd, 1/4th, 1/5thand 1/7th of LC50) of Pb, Cr and Cd, separately, for three weeks. Blood sample of fish was collected to see the micronuclei (MN) and deshape nuclei (DN) in peripheral erythrocytes of fish after one-week interval. Fish W. attu showed significant induction of MN and DN in blood due to metals (Pb, Cr and Cd) exposure. It was noted that Pb and Cd exposure caused significant formation of MN and DN in RBCs ...
Tanzeela Riaz1, Farah Rauf Shakoori2*, Syed Shahid Ali2 and Mushtaq Ahmad Saleem1
...olism and macromolecular concentrations in two larval instars (4th and 6th) of the khapra beetle, Trogoderma granarium, at different exposure periods (24-120 h) was determined. Two populations of khapra beetle used in this research possessed different levels of susceptibility to phosphine. Based on LC50 one population was termed as a susceptible population (never exposed to phosphine previously) while the other was a tol...
Chao-Qun Shi1, Hong-Ying Lin1, Jin-Qing Zheng1, Fan Yang1, Kwame Ayisi Lartey1, Dan-Ju Kang1, Hwa-Chian Robert Wang2, Ravi Gooneratne3*and Jin-Jun Chen1*
...ncreased the IgA and IgG concentration (P<0.05), reversed the intestinal mucosal damage caused by E. coli, and increased the relative expression of TGF-β1 mRNA. Therefore, TFCO has potential to improve immune function against E. coli infection, restored the intestinal structure and cured the E. coli-induced diarrhoea in mice.
...

Asim Faraz1*, Abdul Waheed1, Nasir Ali Tauqir2 and Muhammad Shahid Nabeel3 

...ein, urea and creatinine concentrations varied non-significantly among animals. While the calcium and phosphorus concentration (P<0.05) was found to be 9.88±1.16, 4.77±0.8 and 9.06±1.18, 4.06±0.9 mg/dl respectively in August and February, lower in rut-camels. Such biochemical investigations are evident of the importance of serum bio-gram which has pronounced effect on sexual behavior and may be...
Mengke Wang1, Shucheng Huang2, Cai Zhang1*, Haizhou Gong1
Shunan Cuan1, Shuaishuai Wang1, Qi Shao1, Wenhao Xu1, Sudan Meng1
Pengfei Li1, Yuqin Wang1 and Zijun Yang1
...d administrated with the concentrations of 0 (control), 25, 50, 100, and 200 mg/L of MAG supplementation in the drinking water, respectively. The histological analysis results by Oil Red O and TUNEL staining showed that lipid deposition and hepatocytes apoptosis in the MAG treated groups were significantly lower than that in the control group. Blood biochemical indexes also revealed that MAG supplementation significantly decreased the activity of alanine amino...
Li Ma1 *, Xu Han1, Bo Yang2 and Chunhua Zhou3
...total of 50 µmol/l concentration of kaempferol can profoundly protect the oxidative damage in HUVECs. However, compared to the oxidation-treated cells, the apoptosis rate was significantly decreased in drug-treated cells. Mechanistically, the percentage of G1 phase and S phase cells in vitamin C and drug groups was decreased, and the percentage of G2 phase cells was increased significantly. Intriguingly, the number of CD105/CD62E-marked HUVECs cells was ...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...d and minimum inhibitory concentration (MIC) to aminoglycosides was evaluated by the broth microdilution method. Moreover, the armA genes were studied by PCR followed by Sanger sequencing. The isolates showed a high rate of resistance to cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones whereas colistin and tigecycline were the most active antimicrobial agents against A. baumannii as none of the isolates was found resistant to th...

Shahid Hameed Khan Khalil1, Adnan Shakeel1, Ghani Akbar1, Muhammad Asif2, Ashraf Khan3* and Zafar Islam1

...of capsicum to different concentrations of municipal wastewater treatments through bioremediation. Four irrigation treatments were applied by using potable and wastewater in different ratios. These treatments were T1, T2, T3 and T4 (100% treated wastewater, 66% treated wastewater and 34% potable water, 34% treated wastewater and 66% potable water and 100% potable water respectively). In treatment T1, the highest yields and crop water productivities of capsicum...

Ayesha Gul1, Mohammad Sayyar Khan1*, Mazhar Ullah1 and Iqbal Munir3

...sferred to different PEG concentrations (0%, 2.5%, 5% and 7.5%). Data was taken after 30 and 60 days of treatment. Relative growth of call showed significant decrease after 30 and 60 days. Control had maximum relative growth (3.33), while calli on 7.5% PEG showed the least growth rate i.e 1.33. Similarly, non-stressed control calli had higher water content i.e. (20%) while 7.5% stressed calli showed lowest water content i.e. (5%). Catalase activity was not sig...
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
...gossypii using different concentrations (5, 10, 20 and 40%) of the most effective botanical extracts revealed that the extract of S. officinalis was most toxic (LC50 = 18.59%), followed by N. indicum (LC50 = 20.27%) and M. longifolia (LC50 = 20.73%). Similar trend of effectiveness was observed regarding their LT50 values. Overall study results demonstrated the biocidal potential of the extracts of indigenous plant species of Soon valley against D. citri and A....
Rosidah Rosidah1*, Angga Nugraha1, Yuli Andriani1, Kiki Haetami1, Olga Anne2 and Agus Heri Purnomo3
... vera extract with a concentration of treatment A 
(0 mg kg–1), B (150 mg kg–1), C (300 mg kg–1), D (450 mg kg–1) and E (600 mg kg–1). The variables observed were in vitro test, LC50-24-h test...

Reena Ambreen1, Sadaf Sarfraz1*, Fouzia Qamar2 and Shomaila Skinadar1 

...actant structure and its concentration on the solubilizing power of surfactant molecules resulting in the exclusion of organic coloring agents/dyes which may serve as contaminants or pollutants. In addition to solubilization phenomena, in the present study foam formation more specifically ion flotation has been discussed to understand the dye-surfactant interactions resulting in the removal of dyes/colored substances.

...
Anwar Khan1, Fareeha Nosheen2,4, Bushra Tabassum2*, Olawale Samuel Adeyinka2, Khurram Shehzad3, Naila Shahid2, Arif Muhammad Khan4 and Idrees Ahmad Nasir2
... were obtained at 100 nM concentration of siRNA 1 and 2, while for siRNA 3 promising knockdown was obtained at 50 nM concentration. For long lasting effect of siRNAs, short hairpin RNA corresponding to siRNA3 was designed and tested against CP-PVX which resulted in a similar pattern of inhibition on RNA expression of target gene as synthetic siRNA3. It was found that mRNA expression of targeted gene was reduced to 91% depict...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...cell volume, haemoglobin concentration, and red blood cell indices. Differential leukocyte analysis revealed leukocytosis, heterophilia, eosinophilia, monocytosis, and lymphocytosis. Serum biochemistry showed marked elevation in aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. Histopathological examination demonstrated deg...
Meiping Deng1, Xiaowen Ye1, Jiashan Wu1, Lijuan Chen1, Xiaoxia Jiang1, Changzheng Zhang1, Peiling Zhou1* and Yi Luo2*
...ation (Period 2), with a concentration-dependent prolonging of reaction times, decreased MAP in Period 1 and increased MAP in Period 2, and elevated maximum changes in MAPs and AUCs. This study provides important evidence that arecoline causes biphasic modulations on systemic BP, which provide basic data for future investigations on the pharmacological characteristics and mechanisms of arecoline action, and raise great concerns regarding the cardiovascula...
Zheng Yan1,2,*, Shuang Liu2, Ai-Qing Liu2 and Hai-Yan Wang2
...he photoaging model. The concentrations of elastin peptide given to the low, medium, and high dose groups were 1.5, 5.0 and 10 mg/animal per day, respectively. Then, skin elasticity was measured using a cutimeter dual MPA 580. The concentraions of three type of collagens, hyaluronic acid and hydroxyproline in skin tissue were also determined. The results indicated that the oral administration of elastin peptide greatly improved the skin elasticity, accompanyin...
Muhammad Mohsin Javed1*, Muhammad Azhar Nisar2 and 

Muhammad Usman Ahmad2 

...e subjected to different concentrations of NaCl and pH for evaluation of power generation. The MFC with 50 mM NaCl concentration showed maximum response and gave 88.9 W/m2 power densities and 569 mV of open circuit voltage. The results showed that NaCl, at its optimum level, plays an important role in increasing the bioelectricity and sustaining the internal resistivity. Six different pH parameters were subjected ...
Muhammad Zahid1, Aneela Zameer Durrani1, Muhammad Ijaz1, Khushi Muhammad2,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal3
...nt changes in Creatinine concentration.In group A clinical biomarkers fever, respiratory problem, lameness, anorexia, and milk production became almost normal after 72 h starting from 24 h, while in group B they went down, but not as significantly as in group A. These results confirmed the use of immune booster along with other therapy to successfully treat the BEF in field conditions.
...
Madiha Nawaz and Shoaib Freed


...6, 6.69) days at highest concentrations. Amongst all isolates, B. bassiana isolate Bb-08 was highly efficient against cotton mealybug with highest percent mortality and lowest LC50 and LT50 values. The study showed that Bb-08 can be used in the IPM of P. solenopsis.
...
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
...ctor alpha (TNF-α) concentration (P <0.01), but ACTH treatment has no significant effect on them. Neither ACTH nor LPS treatment had any significant effect on levels of pulmonary SOD, CAT and MDA secretion, as well as expression of IL-6, TNF-α, IL-10, COX-2 and TLR4 mRNAs (P >0.05). Pulmonary cortisol level was inhibited by LPS (P =0.034), with a similar trend of inhibition by ACTH (P =0.062). Pretreatment with ACT...

Salma Javed1* and Daniyal Siddiq2

...nd 72 h of incubation in concentrations of 0.25, 0.5 or 1%. Maximum mortality was achieved with ethanol extracts followed by aqueous and methanol extracts. In the greenhouse, leaf extracts at 60 days after inoculation produced reduction in root galling, egg masses, and nematode populations in plants in treated containers compared to untreated plants (control) with values increasing as time and concentration increased. During...

Asma Hanif* and Shahnaz Dawar

...s at 100, 75 and 50% v/v concentrations of different potencies including mother tincture (Q), 200C and 30C against root-knot nematode Meloidogyne javanica using hatching and mortality test at different time intervals. Notable results were noticed by Kent-20 and Santonine-43 at all tested concentrations by reducing the hatching and complete mortality of M. javanica after 96 hours, while Cina (Q) showed greater reduction in ha...

Mohammed Abdel-Mageed Abdel-Aziz Abdel-Mageed1, Eman Alsayed Hammad2*, Nashaat Abdel-Aziz Mahmoud1 and Anas Farag El-Mesalamy1

...g on materials property, concentrations and solvents used in extraction. The aqueous extract had the best percentage of mortality and inhibition of egg hatching compared to the acetonic and methanolic extracts. Whereas, aqueous extracts of deadly nightshade, winter cherry and chili pepper at concentration 500 ppm applied to the plants as foliar sprays alone, foliar sprays with chelates and soil drenches were tested against r...

Mahmoud M.A.Youssef and Wafaa M. A. El-Nagdi

...elevated atmospheric CO2 concentration (+100 ppm) and higher temperature (+2oC), tomato shoot dry weight and nematode control were increased compared to prevailing conditions.

...

Riaz Alam1*, Muhammad Sajid2 and Durri Shahwar3

... “Influence of IBA concentrations on the rooting response of olive cultivar in air-layering” was conducted at Olive Model Farm, Sangbhatti, Mardan-Pakistan during the year 2015 using Randomized Complete Block Design (RCBD). Air layering was practiced in cultivars: Frontoio, Manzanilla, Pendolino, Ottobratica and Picual during monsoon season. The de-barked portions at the terminal sides were applied with 0, 1000, 2000, 3000 and 4000 ppm IBA solution...

Muhammad Junaid Yousaf 1, Farhad Ali2*and Fawad Ali2

...adaptations to different concentrations of salt stress (i.e. NaCl) to which it is exposed. When three days old seedlings of maize were subjected to aerial salt stress of concentrations; 0.2%, 0.6% 1.2%, and 2% after a week in soil, the seedlings revealed the antagonistic effects which increased with elevated concentrations of salt stress. The experiment was kept for 5 weeks and the chlorop...
Ziyue Qin1, Zhen Wang1, Ali Mujtaba Shah3,4, Zifan Ning1, Yaofu Tian1, Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Lin Ye1, Diyan Li1, Gang Shu2 and Xiaoling Zhao1,*
...rmance and blood glucose concentrations in faster- and slower- growing chicken (different genotypes). In the present study, birds were restricted to 100% to full feed in faster- and slower- growing broilers groups. Body weight gain, feed consumption, blood glucose concentrations were measured on each week from d 1 per 70, and the weight gain of skeletal muscle and viscera were measured on d 7, 28, 49 and 70. The daily volunt...
Congfen Zhang1,Yingxiao Liu2, Linfeng He2, Fengjun Shi2, Weitang Yao2 and Xuegang Luo2,*
...anyl acetate, which concentrations were 6.25mM, 12.5mM, 25mM. Changes in malondialdehyde (MDA) and reduced glutathione (GSH) and activities of superoxide dismutase (SOD), and catalase (CAT) as well as their corresponding mRNA levels in the fat bodies of the pupae were evaluated. As a result, we found that the contents of MDA were increased at 4 h and then decreased compared with control. And, the contents of GSH were decreased gradually. However, the acti...

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

...es;109CFU/g. The highest concentration was found in Loitta fish and lowest in Ruhi from dried fish. Most species of bacteria isolated were resistant to amoxicillin, penicillin-g, kanamycin, azithromycin, cefuroxime sodium, cephalexin, nalidixic acid, cephradine and erythromycin. The isolates were found to be of medical im­portance. Hence it is considered that a variety of bacterial species can be associated with dried fish related pathogen to animal and hu...

Amina, Muhammad Zahid Rashid and Amina Rashid

...g;C). Different chitosan concentrations i.e. 20% 30% and 40% were coated and stored at 11°C temperature. These fruits were compared with control (non-treated) fruit after 15 days. It was observed that the fruits coated with 40% chitosan showed minimum weight losses of 18.66% at cold storage (11°C) respectively as compared to control where weight losses were 34.50% at cold storage respectively. Treated fruits were firmer (41.67 N) after 15 days storage ...

Muhammad Usman Hanif, Abu Bakar Muhammad Raza, Muhammad Zeeshan Majeed*, Muhammad Arshad, Muhammad Irfan Ullah

...gintioctopunctata. Three concentrations of each synthetic insecticide (i.e. 2.5, 2 and 1.5%) and botanical extract (i.e. 10, 7.5 and 5%) were bioassayed using standard leaf-dip method. Results revealed that spinosad and 10% A. indica aqueous extract exhibited maximum mean cumulative mortality of hadda beetle grubs (i.e. 84.1 and 73.4%, respectively) and adults (i.e. 67.4 and 58.8%, respectively) recorded at 5th day of exposure. Combined application of both the...

Oluwatoyin Adenike Fabiyi

...p=0.05) with the highest concentration (75 mg) of furfural. A reduction in nematode population in soil of treated plants was remarked. The results indicated that furfural could be practically applied in the management of nematode pests of sugarcane, while safe guarding the environment from pollution.

...
Nasir Ali Tauqir1, Asim Faraz2*, Muhammad Arif1, Abdul Rehman1Imtiaz Hussain1, Abdul Waheed2, Gabriele Marino3 and Michela Pugliese3

 

...methionine and threonine concentrations (% of crude protein) were formulated and represented as high essential amino acids (HEAA), medium essential amino acids (MEAA) and low essential amino acids (LEAA) concentrations, respectively. The study lasted for 100 days; first ten days were given for the adaptation to new diets while every six days after every month of the remaining period served as collection periods. The intake (...

Mazhar Ullah and Mohammad Sayyar Khan*

...d fructose] with varying concentrations (2, 4 and 6%) were evaluated for their effect on the tissue culture of sugarcane variety-CP 77/400. For callus induction 2,4-D as an auxin was found to be most effective as compared to NAA. BAP and NAA in combination resulted in high regeneration capacities. Amongst the carbon sources, sucrose was most effective both for callus induction and regeneration. ANOVA revealed significant differences (P ≤ 0.05) amongst the c...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
... with harringtonine at a concentration of 0.25, 0.5, 1, 2, 4 μg/ml. P21 and cysteine-containing aspartic protease 3 (Caspase-3) protein expression in U2OS cells were detected by western blot, thiazole blue (MTT) and plate cloning experiments to determine cell survival and colony formation, flow cytometry to measure apoptosis, and quantitative real-time polymerase chain reaction (qRT-PCR) to assay LINC00857 and miR-340 expression. Bioinformatics and dual luc...
Funda Turan1*, Ihsan Akyurt2 and Sehriban Cek-Yalniz1
...ex ratio. Five different concentrations (0, 25, 50 and 75 mg L-1) of red clover extracts and 50 µg L-1 17 β-Estradiol serving as positive control were applied by immersion method on C. gariepinus larvae for 30 days, and the effects of red clover extract on sex reversal and gonadal development were also examined at the end of 120 days.At the end of experiment, highest feminization (89%) was observed at 50 mg L-1
Farah Deeba1, Hafiz Abdullah Shakir1, Muhammad Irfan2, Muhammad Khan1 and Javed Iqbal Qazi1*
...nts. The optimization of concentration of significantly impacted medium components for chitinase production was carried out using central composite design of response surface methodology. The maximum chitinase production was achieved employing 5% chitin, 0.5% rice straw, 0.05% peptone, 0.02% CaCl2, and 0.05% yeast extract. The utilization of agro-industrial waste (rice straw) not only decrease the production costs of microbial chitinase but can also...
Longjun Guo*, Jiaqi Teng, Juan Wang and Yukun Chen
...spinal fluid Tau protein concentration between the experimental group and the control group (P<0.05), and there was no statistically significant difference in CSF IgG index between the experimental group and the control group (P>0.05). DTI was used to process diffusion tensor data and visually and clearly display the distribution and metastasis of brain nerve fibers or white matter. In terms of leukoaraiosis degree, FA decreased. Where, DR ...
Amr N. El-Shahat1, Refaat G. Hamza1,*, Ashraf M. Mounir1 and Madeha N. Al-Seeni2
...orded in lipid contents, concentration of serum urea, creatinine and uric acid, glucose level, activity of some liver enzymes and liver and kidney TBARS levels with remarkable increases in level of HDL-C, insulin and showing elevation in GSH level and SOD and CAT activities of the liver and kidney of gamma-irradiated treated rats compared to irradiated group.It was suggested that the biochemical and antioxidant ameli...
Syed Sikandar Habib1*, Saira Naz2, Sadia Nawaz1, Iqra Ameer1, Ameer Khatoon1, Hameed Ur Rehman3, Sahibzada Muhammad Jawad4 and Haider Ali5
...in, mean cell hemoglobin concentration, white blood cells, lymphocytes, mid-range cells, granulocytes and other less important parameters including platelets, red cell distribution width-standard deviation, red cell distribution width-coefficient of variation, platelet distribution width, mean platelet volume, Plateletcrit and Platelet-Large cell ratio are differed significantly (P<0.05). Similar trend was observed in fishes of both selected sites “Fa...
Asim Faraz1*, Abdul Waheed1, Nasir Ali Tauqir2 and Muhammad Shahid Nabeel3
...espectively. Haemoglobin concentration (P<0.05) was found to be 16.4±0.14 and 16.8±0.09 (g/dl) with diet 1 and 2, respectively. Cu and Mn concentrations in wool also differed significantly (P<0.05) between two groups and were found to be 7.4±0.3, 8.1±0.19 and 48.4±1.1, 54.0±1.5 mg/dL with diet 1 and 2, respectively. The results indicate that Marecha has a good growth potential ...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...nder, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed at the comparatively lower concentration (0.1-1.5%) on the post-thawed quality indicators. On the other hand, the hi...

Sanjeela Sabahat1*, Juliya Abbasi2, Mushtaq Ahmad1, Saima Mumtaz1, Taj Naseeb Khan1, Sudheer Tariq1 and Muhammad Imran1

... and boron, at different concentrations under tunnel conditions on strawberry cv. Chandler at Horticultural Research Institute (HRI), National Agricultural Research Centre (NARC), Islamabad. The experiment was laid out in a randomized complete block design with three blocks. Our study showed that the 0.2% ZnSO4 and 0.4 % FeSO4 showed maximum mean values for fruit weight (18.22 g), fruit width (32.66 cm), yield per plant (175.84 g), no. of fruits per plant (10....

Vahid Mollasadeghi1,2* and Samaneh Elyasi1,2

...was treated with control concentrations (zero), 1, 2 and 3 per thousand. PVY-activated tobacco leaf extract was used to infect the potato plant. This paper measured chlorophyll a, b, leaf area, number of leaves, stem length and tuber weight. The results showed that the symptoms of PVY were completely distinguishable on the leaves of tobacco plants compared to PVY on the leaves of Agria. PVY-infected leaves were more curled and paler than healthy leaves of cult...

Noha M. El-Shabrawy1, Atef M. Kamel2, Aza S. Goda1, Gehad R. Donia1, Ahmed M. Salah-Eldein2* 

...et trap to determine the concentration of lead (Pb), cadmium (Cd), copper (Cu), zinc (Zn) and manganese (Mn) in their muscle, liver, kidney and feather. Also, 16 water samples were collected to assess the level of heavy metals pollution in the study area. The result revealed that the kidney is the most tissue burdens the highest concentration of heaviest metals among the examined tissues. Egyptian barn swallow and house spar...
Nacira Djabali1*, Souad Dougat2, Wahida Haddadi2 and Lamis Fadhli2
...quality, decreased sperm concentration, mobility and vitality, with increased sperm morphological malformations. The treated individuals show hypogonadism and severe tissue damage, especially in group treated with 150ppm dose.
...

Zahoor Hussain1,2, Yasir Iftikhar3, Mustansar Mubeen3, Muhammad Zia Saleem3, Muhammad Umer Naseer3, Muhammad Luqman4, Raheel Anwar5, Faheem Khadija6 and Aqleem Abbas7

...Manganese sulfate at the concentrations of 0.25 g/L, 0.5 g/L, and 0.75 g/L were applied on selected diseased plants in combination. Afterward, different vegetative, physiological, and biochemical parameters of citrus greening infected fruit include fruit diameter, fruit weight, flavedo thickness, total soluble solids, ascorbic acids, juice percentages, TSS/TA ratio, total soluble solids, and titratable acidity were statistically analyzed. The application of Zi...
Ali Ikhwan1*, Dian Indratmi1, Faridlotul Hasanah1, Manar Fayiz Mousa Atoum2,3 and Irum Iqrar4,5
... to examine the type and concentration of purple cleome extract metabolites and understand their effectiveness in inhibiting C. capsici. Purple cleome leaf is extracted with 1:1 w/v absolute methanol and concentrated with 1:1 v/v cold absolute methanol, and then the supernatant is purified using a centrifuge. The metabolic analysis was performed using Gas Chromatography-Mass Spectrometry (GC-MS) and comparing the data with the National Center for Biotec...

Nguyen Binh Truong1,2,3*, Nguyen Van Thu3 

...umen pH, N-NH3, and VFAs concentration values before (0h) and 3h after feeding were similar (P>0.05) among treatments. Nitrogen retention value was numerically higher in NDF47, NDF51 and NDF55 treatments compared with NDF59 treatment (P>0.05) and a similar trend of the observed daily weight gain was found in the treatments. It was concluded that increasing levels of NDF in cattle diets from 47 to 59% reduced DM and OM digestibility while nitrogen retenti...

Prima Mei Widiyanti1*, Mirnawati B Sudarwanto2, Etih Sudarnika2, Raphaella Widiastuti1 

...xacin antibiotic residue concentration, however heating at 121 oC for 15 minutes (sterilization) affected the antibiotic enrofloxacin. The stability of enrofloxacin antibiotic could result problem in public health. Antibiotic residues could result microbial resistance, toxicity, carcinogenicity, allergy, and product rejection. Prevention and control of antibiotic residues should be carried out through socialization to the public, monitoring of antibiotic exami...

Muhammad Iqbal Jakhro1*, Nadeem Sadiq1, Javed Ahmed Abro1, Amanullah1, Fateh Muhammad2, Maqbool Ahmed3, Syed Ishtiaq Ahmed Shah3 and Qasid Hussain4

...l analysis for nutrients concentration, it is concluded from this study that the observed from various of parameters which is growth and developments of olive saplings and soil nutrient status improved through mineral fertilizer when applied in soil during the two periods (three months), besides treatment 5 (NPK 50:25:25) was the superior in this concern.

...

Fang Zhao, Gen Wang, Xiaobin Li, Guodong Zhao, Hui Chen, Chen Ma and Kailun Yang*

...ophan (RPT 5-HTP) on the concentration of 5-hydroxytryptophan (5-HTP) and melatonin (MLT) in the plasma of sheep. Eighteen sheep were assigned randomly to three diet groups (n = 6). The treatment groups included control (CT, corn-soybean meal basal diet), CT + 111 (CT + 111 mg/kg BW RPT 5-HTP), and CT + 222 (CT + 222 mg/kg BW RPT 5-HTP) groups. The experiment lasted for 16 d. On the 16th day of the experiment, blood samples from the sheep were collected at 0 h...

Syed Sikandar Habib1*, Saira Naz2, Sehar Khalid3, Rimsha Kanwal2, Iqra Ameer1, Sadia Nawaz1 Aamir Khan4, Abid Ur Rehman5, Muqaddas Kousar6, Shafa Ullah Khan7 and Nadia Nazir7

...as prepared with variant concentration of mushroom such as 0% (control group) and 2%, 4% and 6% of extractions for the other three groups. Duration of the experimental work was six weeks. Water quality parameters including temperature was 29 ± 0.5, DO: 5.7 ± 0.04 and pH: 7.5. Results revealed that the 6% Agaricus bisporus has shown higher lysozyme activity and ACH50 level, while no significant enhancement was observed in Ig. Beside this all the h...

Li Wei, Wei-wei Shao and Zhi-hua Lin*

...ndividual pesticides. At concentrations of 1 and 2 mg/L, the survival rates of F. limnocharis tadpoles did not drastically differ under treatment with all four individual pesticides. In contrast, the survival rates of M. fissipes showed significant differences under treatment with three of the four pesticides (except for pymetrozine). Our results suggested that individual pesticides and their combinations exerted different effects on organisms and implied the ...

Arkan B. Mohammed*, Ammar S. Abdulwahid, Samah M. Raouf 

... lower serum cholesterol concentration than those of the control. Supplementing a laying hen’s diet with thyme significantly increased glutathione, while, decreased the malondialdehyde, AST and ALT activity in comparison to the control. Therefore, it can be concluded that thyme additives can be used in laying hens diet to improve egg production, egg quality, antioxidant and biochemical parameters in a dose-dependent manner.

 

Keyword...

Elfadil Elfadul Babiker1, Khalid Ahmed Abdoun2*, Fahad AL Juhaimi1, Kashif Ghafoor1, Riyadh Salih Aljumaah2 and Ahmed Abrahim Al-Haidary2
... oxidative state and the concentrations of vitamin C, glucose, and cholesterol in the serum of ewes and goats fed on alfalfa hay-based diet (AHD) supplemented with 25% Moringa oleifera (MOD) or Moringa peregrine (MPD) leaves. Thirty ewes (2 years old and 50-60 kg BW) and 30 goats (2 years old and 35-40 kg BW) were randomly allocated to 3 experimental groups, consisting of 10 ewes and 10 goats each. The 3 experimental groups of either ewes or goats were fed on ...

A.M. Abdul Azeem, Ashraf M. Mounir and Amr N. El-Shahat*

...obvious reduction in the concentration of insulin, total thyroid hormones triiodothyronin and thyroxine, leutinizing hormone, testosterone and testicular antioxidant parameters (glutathione content , the activity of xanthine dehydrogenase, superoxide dismutase and catalase) in the group of alloxan (150 mg/kg B.WT)-administrated rats as compared to control group. Treatment of diabetic rats with GPSDP (1 g/kg B.Wt/day/ 8 weeks) resulted in significant reduction ...

Mirza Gul1*, Muhammad Zahid1, Ikram Ilahi2, Hazrat Ali2*, Fida Hussain3 and Muhammad Anwar Sajad3

...as found between extract concentration and mosquitocidal activity. Regarding predatory control, it was found that during a 12-hour laboratory study, A. cybister consumed 10 exposed larvae. Under field conditions, introduction of predator decreased the larval density from 141.7 to 71 in 15 days. In conclusion, these plants and predator may be useful in controlling mosquito populations in an eco-friendly way.

...

Xiaoli Cai1,2, Saeed Ahmed3, Zhixin Lei1,*, Jianping Wu1,* and Taolei Sun1,2,*

...the cell and improve the concentration in the target tissue. In the current review, the purpose is to find a more effective nanometer system to fight against intracellular bacterial infection. Current observation describes the drug resistance mechanism of bacteria, the bactericidal mechanism of antibiotics, the way of nanoparticles overpasses the cell membrane and approaching the cell, the strategies, prospects and challenges of nanoparticles treating intracel...

Ziyue Qin1, Ali Mujtaba Shah2, 3, Qing Zhu1, Yan Wang1, Diyan Li1, Gang Shu4, Yaofu Tian1 and Xiaoling Zhao1*

...rials, the blood glucose concentration was determined at 0, 10, 20, 40, 60, 120, and 180 min through small brachial blood vessels. As a result, the blood glucose of both group was increased immediately after being treated with glucose and reached peaks at 20 min, then recovered to the normal at 60 min. Birds treated with glucose had greater blood glucose concentration and area under the curve (AUC) in SG than those of FG (P ...

Tran Thi Bich Ngoc1,2, Ninh Thi Huyen1,2, Nguyen Cong Oanh2, Pham Kim Dang2* 

...y, and fecal noxious gas concentration in grower-finisher pigs. Twenty-four barrows [(Pietrain × Duroc) × (Landrace × Yorkshire)], with an average initial body weight of 26 ± 0.77 kg (around 75 days old), were equally divided into four dietary groups, 6 replicates each group. The animals were housed individually in metabolism cages. The four experimental diets consisted of a basal diet (CONT), PROB diet (CONT + 0.2% PROB), ORAC diet (C...
Shehzadi Saima1*, Faiza Ghaffar1, Ghulam Yasin1, Muhammad Nawaz2 and Khalid Masood Ahmad1
...inations under high salt concentration is very vital. With this object we observed the effects of various levels of NaCl (0, 25, 50 and 75 mM) on six varieties (Green leaf, Rama posa, Arka anamika, Super green, Okra kashish and Nerali) of Okra. The results clearly indicated that seeds of all varieties can tolerate the lower concentration of salt (25 mM) and higher (50 mM) greatly reduced the seeds germination while at highes...

Altaf Mahmood1*, Muhammad Athar Khan2, Saima Parveen3, Tanveer Hussain4 and Ayesha Azad5

...with 0.25% free gossypol concentration was daily given to each rabbit at the rate of 4 grams per kg live weight for entire experiment period of 60 days. Analysis of fortnightly collected blood specimens revealed significantly (p <0.05) decreased hematocrit, total red blood cell count, mean corpuscular volume, haemoglobin concentration and inorganic phosphorous level of serum whereas significantly (p < 0.05) decreased m...

Ho Le Quynh Chau, Than Thi Thanh Tra, Duong Thi Huong, Du Thanh Hang, Le Thi Thu Hang, Vo Thi Minh Tam, Dinh Van Dung*, Ho Trung Thong 

...e dietary SID methionine concentration (low, adequate, high). The methionine levels were set up by the recommendation of Evonik (2010) for coloured chickens. At 12 weeks of age, 8 male and 8 female chicks per treatment were randomly chosen for the carcass yield, meat quality, and myogenic gene expression evaluation (myostatin, myf5, MEF2B). The results showed that the higher level of dietary SID methionine than that of Evonik recommendation (2010) improve...

Seon-Ho Kim1, Mahfuzul Islam1,2, A-Rang Son1, Sang-Suk Lee1* 

...t; 0.05). Higher acetate concentration was recorded in the Jersey steers and those received HF diet; however, the Holstein steers and those received HC diet had higher propionate concentration (P < 0.05). The lowest A:P ratio was observed in the Holstein steers and those received HC diet (P < 0.05). From Metataxonomic analysis, Bacteroidetes and Firmicutes were the most abundant bacterial phyla in both breeds and diets...
Warkoyo Warkoyo1*, Devi Dwi Siskawardani1, Anggit Ayu Pradana Siwi1, Moch. Wachid1, Ivar Zekker2 and Jumpen Onthong3
 
 
...ub>3O10 concentrations on the physical and mechanical edible film. Randomized complete block design (RCBD) factorial was applied with three replications. The 1st factor was glycerol concentration (17.5, 22.5 and 27.5) % v/w and the 2nd factor was STPP concentration (0.1, 0.2 and 0.3) % w/v. The results indicated a significant interaction between the a...
Dyah Roeswitawati1*, Iva Kristova1, Muhidin Muhidin1, Otto Endarto2, Manar Fayiz Mousa Atoum3,4, Irum Iqrar5,6 and Luqman Ali Shah7
 
...s the effect of type and concentration of natural pesticides soursop (Annona muricata L.) leaves, papaya (Carica papaya L.) leaves, chrysanth(Chrysanthemum morefolium Ramat.) leaves on the persistence of red mites. This research was conducted at the Research Institute for Citrus and Tropical Fruit Plants in Batu City, East Java, Indonesia from July to September 2017. The study was a factorial experiment, the 1st factor is the ty...
Damat Damat1*, Roy Hendroko Setyobudi2, Juris Burlakovs3, Zane Vincēviča-Gaile4
Devi Dwi Siskawardani1, Rista Anggriani1 and Anas Tain5
... of various seaweed pulp concentrations and spice formulations on the characteristics of analog rice. Various formulas of concentrated seaweed pulp (5 g, 10 g, and 15 g) and spice formulations (onion, garlic, ginger, turmeric, and lemongrass) were applied, and a Randomized Block Design with three replications was used. Subsequently, observation variables included analysis of water content, ash, carbohydrate, protein, fat, antioxidant activity, fiber content, w...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...add45g genes in WBC. The concentration of the viral p27-FeLV antigen in the blood plasma was determined by ELISA. The total DNA and total RNA was extracted from the WBC. The cDNA was obtained using an mRNA matrix. Qualitative and quantitative analyses of FeLV in WBC as well as expression of genes of interest were conducted using real-time PCR (qPCR). Results. Cats with initial signs of leukaemic process activation in WBC showed the following: 1) enhanced FeLV ...

Ahmad-Ur-Rahman Saljoqi1, Sumayya Amin1, Muhammad Salim1*, Taufiq Nawaz2 and Farida Anjum3

...ly highest ascorbic acid concentration (9.68 mg/100g) was recorded on day 10 while lowest (9.19 mg/100g) was recorded on 3rd day post treatment. Similarly, highest percent concentration of pesticide residue (0.080%) was recorded on day 1 while lowest (0.0624%) was recorded on 10th day of post treatment. Results showed that Imidacloprid is significantly better against tomato fruit worm by reducing the fruit infestation, resul...

Hung Van Mai1, Tuan Duc Nguyen2, Nang Thu Tran Thi3 and Hung Phuc Nguyen4*

...contents, bile acid (BA) concentration, lipase activity, and lipid apparent digestibility coefficient (ADC) in pompano. The FSBM1 was produced by fermentation of defatted soybean meal (SBM) with Bacillus subtilis B3. The FSBM2 and FSBM3 were commercially available products, which were produced by fermentation of SBM with Lactobacillus spp. Five diets were formulated and denoted as FMD (fish meal-based diet), SBMD (SBM-based diet), FSBM1D (FSBM1-based diet), FS...

Wafaa M.A. Ghoneem, Reham R. El-Tanany and Adel E.M. Mahmoud*

...s tended to increase but concentrations of ruminal NH3-N and TVFA tended to decrease as the level of zeolite increased. Concentrations of plasma urea were significantly (P<0.05) decreased with lambs fed zeolite rations. Results indicated that zeolite addition had no adverse impact on lambs’ health which all blood parameters were within the normal range. Lambs fed 2% zeolite ration had the highest ADG and the best fe...
Abdul Jabbar1, Muhammad Tariq1*, Asim Gulzar1, Tariq Mukhtar2 and Tayyaba Zainab3
...se in time intervals and concentrations, the mortalities increased showing direct positive relationships between mortalities and time intervals and concentrations. The lethal and sub lethal effects of plant extracts on the mean development of larvae was the maximum by A. indica while the effects on pupal period were the maximum by A. indica and Z. officinale. Similarly, female longevity was the maximum by A. indica and that ...

Amir Z. Allam, Hayam A. Mansour, Nermeen M.L. Malak, Heba H.S. Abdel-Naeem* 

... the effect of using low concentration (0.002%) of thyme (T), oregano (O), clove (CL), and their combination (TO, TCL, OCL, and TOCL) on the bacterial quality of chicken meat patties during frozen storage at –18 °C for 12 weeks by enumeration of aerobic plate count (APC), psychrotrophic, Enterobacteriaceae, and lactic acid bacterial (LAB) counts. The results revealed that the sole effect of T, CL, and O oleoresins caused non-significant reductions in...

Syed Haseeb Ahmed Shah, Irfan Shahzad Sheikh, Abdul Samad, Muhammad Kamran Taj, Mohammad Masood Tariq*, Majed Rafeeq, Sumaira Fazal, Niamatullah Kakar, Sabeera Afzal and Asadullah

...binations with different concentration. Chicks were reared in floor pens bedded with saw dust litter, optimal husbandry conditions for broiler rearing were provided to the birds and vaccinations against Newcastle disease and Infectious bursal disease (IBD) were done at 9th, 19th and 11th, 23rd day, respectively. The results of current study revealed that there was non-significant difference (P>0.05) among treatment groups in feed intake and relative viscera...

Nafisa Shoaib* and Aisha Majid Ali

...er exposure to different concentrations of organophosphate pesticides (chlorpyrifos, malathion), synthetic pyrethroid pesticide (cypermethrin, lambda-cyhalothrin) and herbicide (buctril). Haemocytes of bivalve play an important role in immune defense and detoxification of contaminants. The bivalves were exposed to different concentrations of test pesticides in a static system. The MN frequencies of all the pesticide treated ...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...Gallic acid in different concentrations and in other animals. 

...

 Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4

...alcium and phosphate ion concentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiated osteoblast cells derived from isolated rBM-MSCs by flowcytometric analysis was studied by histochemical staining with Alizarin Red and von Kossa to confirm the osteogenic differentiation process. Also, cell viability assay by MTT was used to measure the number of viable osteoblast cells cultured with scaffolds extracts. Also, the antitumor activit...
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
... hazards of tetracycline concentrations through study the influence of simmering and frying on these residues. Quantitative determination of tetracycline residues was done using High Performance Liquid Chromatography. The results revealed that the incidence of tetracycline residues was 260 (87%) in all examined chicken meat. The average concentration values of oxytetracycline (OTC), tetracycline (TTC), chlorotetracycline (CT...
Ghada Mohamed Safwat1*, Mohamed Ahmed Kandiel1, Omayma A.R. Abozaid2, Mahmoud Mohamed Arafa3, Sahar Omar Mohamed4
...atty acids, antioxidants concentrations in serum and/or egg yolk and on gene expression of fatty acid synthase and acetyl CoA carboxylase in laying hens. The experiment was applied on 42 Commercial Mandarah strain laying hens which divided equally into control and olive cake (7%) group.Addition of olive cake (7%) led to a significant increase in serum and egg yolk HDL-C and a significant decrease in TAG, cholesterol, LDL-C and VLDL con...

Semra Okur Gumusova1, Gul Fatma Yarim2, Cuneyt Tamer1,*, Ayris Salt2 and Gokhan Inat3

...nd malondialdehyde (MDA) concentrations were measured by the spectrophotometric methods. Data obtained revealed higher MDA levels in IPNV infected cells than negative control medium (p<0.001). However, SOD and GPx levels in IPNV infected cells were lover than negative control cells medium (p<0.001). These results indicate that, cell damage in RTG-2 cell line, that is caused by IPNV is assosiated with oxidative stress.

...

Qaisra Siddique1*, Sajid Abdullah1, Huma Naz2, Khalid Abbas1, Laiba Shafique1 and Qingyou Liu3

...mparison among different concentrations indicated that 1/3rd of LC50 caused greater increase in GST activity followed by that of 1/5th and 1/6th. The GST activity in selected tissues of fish varied with duration of exposure as 28>21>35>42>14>7>49>56. Total protein contents in all selected tissues of CPF exposed fish were decreased with the passage of time. Among all the concentrations, 1/3rd of LC50 caus...

Mudassir Safdar, Aurang Zeb, Sidra Khalid, Shahid Bashir, Riffat Mehboob* and Muhammad Imran

...bjects. Blood hemoglobin concentrations and anthropometric measurements of all participating hostel girls (n=219) were determined initially (Day-0) and at the end of 4 months feeding period. Mean age of the subjects was 21.88 years, with an average initial BMI of 24.71 kg/m2. Findings of the efficacy trial showed that the average hemoglobin level in anemic girls increased from 8.99 to 9.94g/dl. The prevalence of severe anemia was reduced from 8 to 4% (p < 0...
Soliman Mohammed Soliman
...t value in rumen ammonia concentration and protozoal population. Furthermore, the addition of probiotics or green seaweed to the diet has significantly improved milk yield and composition compared with yucca supplementation, as well as decreased the somatic cells count (SCC) as a result of feed additives. Experimental feed additives contributed to enhancing beneficial processes and the reduction of methane production.

Nuzhat Naseem, Sajid Abdullah and Sana Aziz*

... supplementing different concentrations of phytase. i.e. D1 without phytase supplementation, D2, D3, D4, D5 and D6 with 250, 500, 750, 1000 and 1250 FTU phytase per kilogram of diet, respectively. An experiment was conducted for 2 months. During this experiment, water quality parameters were also examined e.g pH, Dissolved oxygen and temprature. Results were examined by Tukey’s Honestly Significant Difference Test. Phytase supplementation decreased the m...
Afsheen Noman Saddar1, Anila Naz Soomro2*, Sadaf Tabasum Qureshi1, Syeda Saleha Hassaney1 and Mukhtiar Ahmed Mahar2
...1 g/l and 1.5 g/l). Five concentrations (1200, 2500, 5000, 10000 and 15000 µg/ml) of aqueous extracts of five fertility enhancing plants were tested for 24 h incubation to check mortality of brine shrimp nauplii. Mean comparison of brine shrimp cytotoxicity assay revealed noticeable deviations from the both positive and negative controls. Alive percentage of nauplii observed in the aqueous extract of sweet flag root and radish seed ranged from 0 to 10%, ...

El-Kholy KH1, Tag El-Din H1, Tawfeek FA2, Sara HM Hassab1* 

...ntrol group. The highest concentrations of these values were observed in dose 7.5 ml SLAE/kg diet. Also, SLAE addition result a dose-dependent decrease (P<0.05) in ACTH levels in laying hens. The treatment did not affect T4 and LH hormones (p=0.1470 and 0.2764, respectively). Regarding reproductive traits, the treatment increased the hatchability to total and fertilized eggs, maximizing in groups treated with 5 and 7.5 ml SLAE/kg diet. There was a non-sign...

Hassan Ali1, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1* and Muhammad Imran Hamid2

.... Similarly, the highest concentration (15%) of C. reticulata extract exhibited maximum repellency (88%) of larvae, followed by Datura stramonium (84%) and Azadirachta indica (79%) at 24 h post-exposure. Regarding bioassays with microbial insecticides, maximum mean mortality of larvae (i.e. 42.6 and 46.1%) were exhibited by the highest concentration of Bacillus thuringiensis (18000 CFUs mg-1) and Lecanicillium lecanii (1.0 &...

Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar 

...n and fecal calprotectin concentration as prognostic markers in dogs with IBD. After a detailed clinical, laboratory and ultrasonographic examination 21 IBD dogs with symptoms of chronic gastrointestinal diseases were chosen for the study. In addition to 11 healthy dogs served as control group. In comparison to controls, hematological analysis revealed significant variations (p<0.05) in total leukocyte count, hemoglobin, and mean platelet volume and signifi...

Arman Sayuti1, Hamdan Hamdan2, Miftahul Jannah3, Safriadi Safriadi3, Teuku Nasri3, Tongku Nizwan Siregar2*

...spring per birth and the concentration of steroids (estrogen and progesterone) in the local rabbits. In this study, we used 10 non pregnant rabbits that have given birth and were healthy clinically. All rabbits were randomly divided into two treatment groups, namely control group (K1) and treatment group (K2) where each group consist of five rabbits. The rabbits of K1 group were injected with aquabides for three consecutive days at a doses of 1.0, 0.5 and 0.3 ...

Mohamed Abd El-Fattah Abo-Farw1, Maged Ahmed Aboul-Omran1, Abdel-Khalek El-Sayed Abdel-Khalek2* 

...ficantly decreased serum concentration of progesterone (P4), estrogen (E2), thyroxin (T4), zinc (Zn), and selenium (Se) by about 24.05, 13.80, 5.36, 2.50, and 3.44%, respectively, while triiodothyronine (T3) concentration showed non-significant decrease by about 3.77%. Mastitis incidence at early postpartum in lactating Egyptian buffaloes delayed the resumption of estrous and ovarian activity and decreased pregnancy rate, an...

Evrim Sönmez

...the effects of different concentrations of Beauveria bassiana (Nostalgist BL®) on the mortality rates of A. obtectus adults, the number of eggs they lay and the number and percentages of adults emerging from bean seeds were evaluated with dipping and spraying method. It was found that as the concentration of B. bassiana, which was applied at concentrations of 0.1%, 0.2% and 0.4% increa...

Muhammad F Tajol Ariffin1, Chai M Hian1, Muhammad Z Sukiman1, Mohd F Ghazali1, Siti M Zainal Ariffin2* 

...relation between milk Hp concentration and SCC was observed (rs= 0.73, p<0.05). However, there were no associations between milk SAA and AGP concentrations with SCC respectively. In conclusion, altered expression of the serum and milk APPs was seen during S. aureus-induced subclinical mastitis infection in goats, which unlock the potentials of APP evaluation as alternative tools for early diagnosis of subclinical mastitis...

Ji Xu, Zhonghua Liu, Zhenhai Cui and Wenhai Zhao*

...e treated with different concentrations of GSTT. ELISA was used to detect the levels of IL-6, TNF-α and IFN-γ. Flow cytometry was used to detect the apoptotic rate. The expressions of Bax, cleaved-caspase3 and Bcl-2 were detected by Western blot. qRT-PCR was used to detect the effect of GSTT on miR-99a expression level. The above methods were used to detect the effects of miR-99a overexpression on IL-1β-induced chondrocyte inflammatory factors...

Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1

...sperm (volume, motility, concentration, survival) of rams bred in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 1...

Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1

...sperm (volume, motility, concentration, survival) of rams bred in mid-latitude. Sperm samples were collected from 5 Dzhalginsky merino rams once a week for twelve calendar months using an artificial vagina. The lowest quality sperm was produced in the spring, high quality indicators were observed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 1...

Mariam Arain1, Asghar Ali Kamboh1* and Muhammad Javed Arshed2

...y higher (p<0.05) IgG concentration was found in Se supplemented goats as compared to control group. The bacterial species produced weak positive biofilm formation (p<0.05) in Se supplemented goats as compared to control group. The hematological indicators revealed that the values of hemoglobin, hematocrit, erythrocytes, mean cell hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, leukocyte...

Tiyyabah Khan1, Hafiz Azhar Ali Khan1*, Muhammad Rizwan Khan1, Muhammad Umer1, Adnan Akhter1 and Waseem Akram2

...mortality at the highest concentrations of spinosad (1 ppm) and thiamethoxam (4 ppm), while >95% mortality was recorded at the highest concentrations of imidacloprid (4 ppm) and indoxacarb (4 ppm). In the case of T. castaneum, about 51%, 98%, 54% and 62% mortality were observed at the highest concentrations of spinosad, thiamethoxam, imidacloprid and indoxacarb, respectively. Correlatio...

A. Samy1*, H.M.A. Hassan1, Fatma T.F. Abd-El Ghany2, Shama H. Morsy2 

...r garlic extract (GE) at concentrations of 200 or 400 ppm were added to the other diets. The results revealed that adding curcumin or garlic extract to growing rabbit diets boosted significantly meat crude protein (P<0.05) while, decreasing moisture and fat levels. Digestibility coefficients of CP, CF, and EE, as well as the nutritive values of TDN, DCP, and DE, were considerably improved (P<0.05), although DM, OM, and NFE digestibility was unaffected in...

Ayu Cusiayuni1, Risan Khaerani Nurfatahillah1, Rakhmad Perkasa Harahap2, Komang Gede Wiryawan3, Dwierra Evvyernie3, Anuraga Jayanegara3* 

...nded to decrease ammonia concentration (P < 0.1). Lauric acid also reduced dry matter digestibility (P < 0.05). It is concluded that lauric acid is an effective agent for mitigating methanogenesis in the rumen.

Keywords | Lauric acid, Meta-analysis, Methane, Rumen 

...

Rabia Bibi1, R. Muhammad Tariq2, Samir A.M. Abdelgaleil3 and Munawwer Rasheed1, 4*

...ethanol). Five different concentrations of the extracts were subjected to the toxic assessment against two species of stored grain pests (Sitophilus oryzae and Callosobruchus maculatus). Results obtained from experimental trail proved that the C. maculatus were susceptible with higher adult mortality than S. oryzae. The highest toxic effect was induced by dichloromethane obtained extract from A. taxiformis (DA) against C. maculatus (LC50 1.15 mg/cm2) after 24 ...

Lin Lu

... treated with HDP (final concentration 1 mg/mL, 2 mg/mL and 4 mg/mL), PI3K/AKT signal pathway inhibitor LY294002 (20 μmol/L) and LY294002 + HDP (4 mg/ml HDP and 20 μmol/L LY294002). Cell viability and apoptosis rate were detected by MTT and flow cytometry, respectively. The expression of Cyt-C, Bcl-2, Bax, cleaved Caspase-3, PI3K, AKT and p-AKT were detected by Western blotting. We found that activity of TPC-1 cells was inhibited by different

Fan Xu1,2*, Cheng Wu3 and Su Zeng1

...mpatibility of different concentrations, the changes of pH, particle size distributions, the particles greater than 5μm and encapsulation rate of the compatibility solutions were observed within 8 h by using electronic acidimeter, Malvern zeta sizer nanoparticle size analyzer, accusizer APS 780 particle size analyzer and Agilent 6460 LC-MS/MS mass spectrometer. From the experimental results, 5 μg:50ml group kept good targeting within 6 h. The encapsulati...

Jabbar Khan1, Mehwish Jehan1, Zeeshan Mutahir2, Muhammad Rafi1, Muhammad Ismail1, Aamer Abbas1 and Jabbar Tanveer3

...ormones. Beside that the concentrations of total protein, cholesterol, albumin and glucose were found significantly higher in Damani breed than control group, indicating that Damani breed had comparatively better adaptive capabilities in preparing the internal physiology and metabolic processes in response to heat-stress environmental conditions. Hence, vitamin E, in combination with Se improved the physiological and biochemical profile of blood in Damani goat...

Nahla Hamada Magd Khalil1*, Ihab Mahmoud Helal2, El-Desoky Hassan Ibrahim Dorrah1, Soad Ahmed Soliman Ismail3 

...ish species at different concentrations. Mullet fish samples had significantly (P < 0.05) the highest concentrations of alderin, dieldrin and heptachlor epoxide with mean ± SE concentration of 89±0.85, 36.7±0.66, and 38.9 ±0.49 ppb, respectively. Tilapia fish had the highest concentrations of DDD, DDE and γ-chlordane ...

Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3* 

... by increasing CHIR99021 concentration to 0.01 mmol/l. The pESC primary colonies culture showed that blastocysts cultured in CHIR99021 0.003 mmol/l were able to form a pESC primary colony that was similar (p>0.05) to fertilized blastocysts to form ESC primary colonies. These results were significantly improved (p<0.05) compared to those from blastocysts cultured in CHIR99021 0 mmol/l. The characterization results showed that CHIR99021 0.003 mmol/l showed...

Xian Zhang1, Erjiang Lin1, Shizhe Hong1 and Zhengjie Zhu2*

...nd cultured at 1% oxygen concentration. Western blotting was used to detect expression of SSAT, Akt, GSK-3β and β-catenin proteins; and flow cytometry was used to detect cell apoptosis rate and mortality rate. SSAT was successfully transferred into prostate cancer LNCaP cells. Compared to the blank cell group and the blank vector group, the expression of Akt, GSK-3β and β-catenin proteins in the SSAT transfection group was significantly dow...

Nabila Khan1, Imran Ahmad2 and Muhammad Bilal Sadiq1*

...(1:20-1:40 w/v), solvent concentrations (20-80%, v/v) and time (10-30 min), whereas, TPC and DPPH were used as response variables. At optimized extraction conditions (40 ml/g, 22.5 min and 80% of ethanol), the experimental values for TPC and DPPH inhibition were 110.17 ± 3.44 mg of GAE/g of extract and 87.45 ± 1.28%, respectively. For DPPH inhibition assay, IC50 value of almond hull extract was 51.64 μg/ml which was lower than the ascorbic aci...

Alaa Jaheen*, Noha Salem, Mohamed El-sherif 

... and estimation of serum concentration of total antioxidant capacity (TAC), malondialdehyde (MDA), C-reactive protein (CRP), serum zinc, copper concentrations, total protein, albumin, globulin values, triglycerides, and cholesterol concentrations. The most consistent hematological alteration was elevated eosinophils in eczema-affected horses. The oxidant-antioxidant status showed a signifi...

Ibrahim Samir Abd El-Hamid1*, Wafaa Adel Abd Fouda1, Hesham Attia Shedeed1, Safaa Ali Mostafa1, Ahmed Mohamed Elbaz2, Salah Abo Bakr2, Baliegh Hamdy Mosa1, Ali Saber Morsy1, Amal Mohamed Hasan1, Khamis Refaay Emam3 

... (TP) and globulin (GLO) concentrations increased (P<0.05) in treatment groups compared with control. Levels of albumin (ALB) and aspartate aminotransferase (AST) decreased (P<0.05) in Tr2 compared with Tr3 or Tr1. While alanine aminotransferases (ALT) and creatinine (CRA) concentrations decreased in treated groups compared with control. Value of total antioxidant capacity (TAC) increased (P<0.05) in treaded groups...

Mubashar Ahmad, Roheela Yasmeen*, Aisha Waheed Qurashi, Muhammad Hassan Ali Shah 

... cortisol levels. Higher concentrations of cortisol were recorded in both males and females of blackbuck in the Jallo Park and Lahore Zoo as compared to Safari Zoo. Moreover, higher cortisol levels were recorded in the blackbuck in summer season as compared to winter season. Results of ANOVA showed significant differences in cortisol levels among different sites at <0.05 p-value. Temperature was positively correlated with the cortisol levels. While humidity...

El-Kholy KH1*, Tag El-Din H1, Seham NE Seleem1, Eman Hussein2 AM El-Shhat2 

...uo;s eggs with different concentrations on body weight at hatch, hemato-biochemical changes and subsequent performance of newly-hatched ducklings. A total of 1560 fertile eggs used in the current experiment were obtained from Sudani ducks flocks of 33 weeks of age. The eggs were randomly distributed into of five different SV solution concentrates; the first (1st) group without any treatment and served as a control (C), the 2nd group was dipped into distilled w...

Muhammad Asif1*, Ahmad Ali Shahid1,2 and Nasir Ahmad3

...s for minimum inhibitory concentration (MIC) of crude protein extract reveal that 5mg/ml is the concentration, which inhibited 100% growth of A. solani. It was concluded that either mycelia or crude protein extract of G. lucidum could be used for pathogen inhibition.
...
Syarif Husen1*, Devi Dwi Siskawardani2, Setiawan Deny Rexmardi1, Erny Ishartati1, Muhidin Muhidin1, Jumpen Onthong3 and Ivar Zekker4
...creasing organic matters concentration in the soil, and it was able to repair air and groundwater systems that supported plant roots growth and optimum capability to absorb nutrients.
...

Jiang Wu*

...nd the initial bacterial concentration was adjusted to about 5×108 CFU/mL. T. officinale were dried in a hot air oven, and chemical substances in the dried powder were separated using Soxhlet extraction method with light petroleum as organic solvent. The antibacterial properties of T. officinale extracts against foodborne pathogens were evaluated by agar diffusion method. Sub-inhibitory concentration (SIC) value, minim...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
...ry effect upon different concentrations. Effect of inhibitors (β-mercaptoethanol, EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular wei...

Shuhuan Li1*, Yongheng Bo2, Youzhi Li3 and Xiuzhen Yang2

...31 % when the additional concentration ranged from 0.5 to 5.0 mg/kg, and a detection limit of 0.25 mg/kg, providing an effective and reliable method for the detection of atropine residues in beef. 
...

Serdar Bektas* and Ozer Ayik

...t decrease in hemoglobin concentration noted only in the 21th day, significantly lower values in hematocrit, were observed at 7, 14 and 21th days, total leucocyte and thrombocyte counts, found significantly higher only in the 3th day, comparing with the control group. While, significantly increasing tendency were determined in mean corpuscular volume and mean corpuscular hemoglobin values from 7th day to the end of the experiment, no significant alterations in...

Luke Chukwudi Ali*, Nnanna Ephraim Ikeh, Bright Chigozie Amaefule, Amarachi Linda Obinna, Ndubuisi Samuel Machebe 

...). Sperm motility, sperm concentration, live and normal sperms in decreased with an increase in dietary DCSM whereas non-motile, dead and abnormal sperms increased with an increase in dietary DCSM. Testicular morphometric measurements and histomorphological evaluations were similar in all treatments (P>0.05). This investigation depicts that DCSM inclusion above 15% affected semen quality negatively even though results on testicular morphometric traits and h...

Wafaa M.A. Ghoneem*, Muhammad M. Shaklouf, Ali M. Ali and Mohammed H. Bakr

...lt;0.05) decreased blood concentration of ALT, AST, cholesterol, triglyceride and urea compared with control. While, lymphocyte transformation and phagocytic index were increased by (18 and 23%) and (20 and 62 %), respectively for G2 and G3 compared with G1. Average daily milk yield was increased by 2.23 kg/d and 0.9 kg/d in G2 and G3, respectively compared with G1. It could be concluded that addition of 10 g LAFP/h/d seems to be profitable whereas, it increas...

Lopamudra Samantaray*, Yashaswi Nayak 

... intakes. The hemoglobin concentration, Mean Corpuscular Volume (MCV), and Mean Corpuscular Hemoglobin (MCH) values of birds fed with 0.5% black pepper and 0.5% turmeric, as well as 1% black pepper, were found to be higher. The Red Blood Cell (RBC) counts were increased with 0.5% black pepper and 0.5% fennel. The study concluded that phytobiotic EOs are cost-effective and safe natural growth enhancers for broiler chickens with health-promoting properties.

...

Shorouk Aladdin Helmy1, Hossam Mahrous Ebeid2*, Mohamed Ahmed Hanafy1, Adel Eid Mohamed Mahmoud1, Reham Roshdy Ali El-tanany1 

... but has decreased NH3-N concentration (P = 0.009) with the cottonseed ration. Bentonite rations had quadratically increased (P = 0.01) in NH3-N concentrations, but the TGP (ml) and TGP (g/kg DM) were linearly decreased with bentonite addition but linearly increased in soybean and sunflower rations. No significant difference was observed for cottonseeds and bentonite addition. The interaction between bentonite and the type o...

Eman Alsayed Hammad1* and Mahmoud Mohamed Hassanin Hasanin2

...f 5.91–9.77 nm) at concentrations 4000 and 5000ppm separately, recorded the best results in vitro investigations, completely prevented F. oxysporum growth at all concentrations, and increased M. javanica mortality by 100%, in comparison to the non-treated control. In the greenhouse, thyme and spearmint essential oils nanoemulsions and emulsions (at 5000 ppm) affected significantly on the final population (Pf) of M. jav...

Md. Enayet Kabir1, Md. Jahangir Alam1*, Md. Mosharaf Hossain2, Zannatul Ferdaushi3 

...lood urea nitrogen (BUN) concentrations and coliform count were significantly (P<0.05) lower in the TMR2 fed group relative to the TMR1 and control ration fed groups. It may be concluded that feeding of rice straw as such or after its probiotic fermentation along with concentrate mixture in the form of TMR produced a better response in fattening cattle than the conventional system of feeding concentrate and straw separately.

Keywords: Total mixed ra...

Feng Xu, Weijing Fan and Guobin Liu*

...ty of rutin of different concentrations (0, 25, 50, 100, 200, 400 μmol/L) on human umbilical vein endothelial cells (HUVECs). HUVECs were treated with 100 mg/L ox-LDL for 24 h for establishing HUVECs injury model, and then treated with rutin in different doses. The cell activity and apoptosis rate of HUVECs were analyzed by CCK-8 method and flow cytometry, respectively. The levels of intracellular catalase (CAT), superoxide dismutase (SOD) and malondialdehy...

Tao Ren1, Guiqiu Cao2, Xiao Han3, Feng Tan1, Qiaoli Chen4, Shicheng Yang5 and Haiyan Zhang6*

...ctive protein (CRP), and concentration of mitochondrial enzymes viz., Ca2+, Na+ and K+ ions were estimated in blood. Heart tissue was also isolated for caspase-3 activity. Our result showed that naringenin pretreatment significantly increased cardiac dysfunction via scavenged free radicals and a reduction of inflammatory reaction. Dose-dependent treatment of naringenin significantly altered the CK-MB, HDL, LDL, creatinine, cholesterol, CRP, Ca2+, Na+ and K+, r...

Amjed Ali1, Muhammad Tayyab1*, Abu Saeed Hashmi2, Asif Nadeem3, Shumaila Hanif4, Sehrish Firyal1, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1

...08% when used at a final concentration of 1 mM. The ionic and non-ionic detergents showed slight inhibitory impact on the recombinant β-lactamase activity. The enzyme exhibited the Km and Vmax values of 2.27 mM and 45.45µmol/min, respectively when benzylpenicillin was utilized as substrate. The degradative ability of recombinant β-lactamase to hydrolyze a variety of β-lactam ring containing antibiotics makes it a suitable candidate for its...

Weam Mohamed Baher*, Gamilat A. El said 

...sin, and gingerol at two concentrations (1%, and 2%). The achieved results indicated an unsatisfactory hygienic status of the retailed chicken meat in the study area, in terms of high microbial counts. Chicken breast collected from rural areas had significantly (p< 0.05) the highest counts. A significant reduction of the microbial load of chicken breast was achieved after treatment with nisin, and gingerol, particularly at 2%. For instances, TBC was signifi...
Nguyen Binh Truong1,2,3*, Nguyen Van Thu3
...tal volatile fatty acids concentration of cattle were no differences and good for the rumen activities. In this research content, the NDF55 treatment revealed an expectation for the applied studies. Results of the second experiment indicated that with the NDF 55% in the diets, the Charolais crossbred cattle showed a superior trend on feed intake, daily weight gain and feed conversion ratio compared to the Black Angus and Wagyu crossbred ones. Therefore, the re...

Soliman Mohammed Soliman1*, Mohsen Mahmoud Shoukry2, Ahmed Mohammed El-Okazy1, Ahmed Mahmoud El-Morsy1, Mahmoud Mohammed Soliman1

...on, and TVFA and ammonia concentrations without effect on physiological rumen activity. Moreover, milk yield and milk composition were not affected. However, the concentration of milk urea nitrogen (MUN) was significantly decreased. Overall, these results indicate that treated SBM with levels of 200 or 250 PP reduced its degradability in the rumen more than untreated SBM. Also, recorded decreased gas and methane production h...

Mimi Syazwani Jaapar1, Eric Lim Teik Chung1,2*, Nazri Nayan1, Mamat Hamidi Kamalludin1,2, Mookiah Saminathan3, Kalai Vaani Muniandy2, Muhammad Hazziq Mohd Hamdan1, Shokri Jusoh1, Faez Firdaus Abdullah Jesse2,4 

...s diet (T3) based on the concentration of saponins. Meanwhile, 100% P. purpureum was used as the basal diet, which served as control (T1). Rumen fluid was then collected from six young male Dorper cross sheep for the analyses of gas production, pH, in vitro organic matter digestibility, ammonia nitrogen, and volatile fatty acids (VFA). All analyses were conducted according to established methods and data collected were subjected to one-way analysis of variance...

Asaad A. Fares1*, Mohamed M. Ghanem2, Yasein M. Abd El-Raof2, Hossam M. El-Attar2, Ameer A. Megahed2, Heba M. El-Khaiat2

...Ca, Mg, P, K, Cl, and Na concentration pre and post calving, compared to non DCAD group. Before parturition, feeding on DCAD ration caused a significant increase in urea and creatinine, but decreased bicarbonate compared to non DCAD group. While after parturition urea, creatinine, and bicarbonate were decreased in the DCAD group, compared to the non DCAD group. Milk production was increased for the DCAD group compared with non DCAD group, but no differences we...

Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby* 

... MIC (minimum inhibitory concentration) method 68.8% exhibited resistance. Colistin was recorded as 33.7% in chicken breast by HPLC. Furthermore, mcr-1gene was detected 54% using PCR. Sanger sequencing revealed the same identity (100%) between the three examined strains despite coming from different sources. It could be concluded that the practices inside poultry farms might be a possible source of spread of antimicrobial resistance to the food chain in Egypt....

Ghulam Abbas1*, Muhammad Arshad1, Muhammad Saeed2*; Safdar Imran3, Ashgar Ali Kamboh4, Duraid KA Al-Taey5, Muhammad Asad Aslam1, Muhammad Saeed Imran6, Muhammad Ashraf3, Muhammad Asif7, Abdul Jabbar Tanveer8, Razia Abdul Majid Qureshi9, Maria Arshad1, Hussain Ahmed Khan Niazi1, Muhammad Tariq10, Sikandar Abbas1 

...hell weight, and calcium concentrations in bones and reduced ammonia concentrations in the caecum. Egg production was increased upto 9.84% by the supplementation of OA. Organic acids also have promising effect on gut health evidenced by positive effect on all the intestinal parameters. Moreover, they known to improve meat production in broilers by increasing the nutrients absorption from gut. This review article discussed al...

Samia Azad1, Iram Liaqat1*, Riffat Iqbal1 and Uzma Rafi2

...a) exposed to sub lethal concentrations of heavy metal cadmium (Cd). CdCl2 was used as cadmium (heavy metal) source. Five concentrations of CdCl2 (0.2, 0.4, 0.6, 0.8 and 1.0 ppm of Cd) were tested in glass aquaria having 35 Rohu fingerlings in each. After exposure for six week, sections of intestine, kidney and liver were excised and studied histologically. Results revealed variation in histological changes from mild to seve...

Muhammad Musa1*, Saba Saeed2, Azher Mustafa2, Muhammad Ussama Yasin2, Saima Naseer2 and Iftikhar Haider3

...ce using three different concentrations (0.25%, 0.50%, 0.75%) with 15-days interval. Although all activators were found effective to manage PLRV with some variations, but maximum disease was managed by salicylic acid with 0.75% concentration. In case of nutrients application, both solutions were found to be effective against PLRV but macronutrients solution with 0.75% concentration was fou...

Noor Muhammad Khan1,2, Tariq Mahmood Khalil1,3*, Rashid Rehan2 and Iftikhar Zeb4

...ng the heavy metals (HM) concentration in the effluents generated from the Hayatabad Industrial Estate and the phytoremediation capabilities of two locally available plant species Arundo donax (A.d) and Typha latifolia (T.l) for removing HM from the untreated wastewater of Hayatabad Peshawar used for irrigation downstream. The wastewater analysis showed that HM concentration exceeds the limits of thresholds values. For phyto...

Hamdy Abdala Elnagar, Wael Mohamed Wafa*, Abdelaziz Mustafa Sakr, Mohammed Mahmoud Hegazy 

Syeda Rubab Zaidi* and Safdar Ali Mirza

...ficantly greater protein concentration than crude sample so, the optimum pH was 5.0 for laccase activity and the enzymatic extract was thermostable up to 60oC for 1 hour with guaiacol as substrate. The biological production of enzyme have an ecofriendly impact in current scenario. 
 
Novelty Statement | The study is novel in suggesting ecofriendly impact of production of laccase enzyme from locally isolated Aspergillus flavus ...

Asim Faraz1, Muhammad Yaqoob2, Nasir Ali Tauqir3, Hafiz Muhammad Ishaq1, Ayman Balla Mustafa4, Amir Ismail5, Muhammad Arslan Akbar6, Abdul Waheed1* and Muhammad Shahid Nabeel7

...-pregnant group serum P4 concentration was recorded to be 1.05±0.29 ng/ml which is considered as basal level of progesterone in dromedary camel. Mean serum estrogen concentration (P<0.05) was found to be 25.28±2.71, 54.80±2.52 pg/ml at 14th and 21st day post mating respectively in pregnant group of animals, while in lactating non pregnant animals E2 level was recorded as 10.38±1.52 pg/ml. Hormo...

Rana Aamir Shehzad1, Ghulam Sarwar1*, Sabir Hussain Shah2, Mukkram Ali Tahir1, Noor-Us-Sabah1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1 and Imran Shehzad1

...acteristics and nutrient concentration of wheat plant compared to control (T1). Use of phosphocompost proved very useful to enhance soil health and consequently nutritional characteristic of wheat crop.

...

Nimra Altaf1, Muhammad Arshad1, Muhammad Zeeshan Majeed1, Muhammad Irfan Ullah1*, Hamza Latif1, Muhammad Zeeshan1, Gulfam Yousuf1 and Muhammad Afzal2

... comparison to different concentrations of single botanical, i.e., neem leaf extract @ (50 ppm and 100 ppm) against FAW larvae in maize. Our findings showed a significant effect (P < 0.001) of these chemicals on the mortality and consumption rate of FAW larvae. Results show that mortality increased in all treatments with time. Recommended dose of chlorantraniliprole (50 ml/100 litre water) insecticide showed 71.0% mortality of FAW on 5th day, which increase...

Nkana Kontchiachou J. Gwladys1, Kouamo Justin2, Vemo Bertin Narcisse3*, Mweugang Ngouopo Nathalie4, Wang-Baa Temoa Christophe1, Awantu Christian Funwi1, Semi Yam Alphonsius1, Kenne Noubissie Christèle5, Tendonkeng Fernand5

...control. The haemoglobin concentration, packed cell volume, mean corpuscular volume of haemoglobin, the number of red blood cells and the concentration of placitonin were comparable (p>0.05) among rations. The number of platelets rose with an increasing level of green anise supplementation, but the significant (p<0.05) difference was observed only at 0.75%. The serum concentrations o...
Sara Mohamed Hemeda1, R.H. Sayed2, Hani Hassan1, Sheima A.E.2, Hassan Aboul-Ella3, R. Soliman3*
...idues in dairy milk. The concentration of the gold chloride-antibody conjugate in the conjugation pad was adjusted to detect β-lactam antibiotics in milk samples that exceed the permissible limit. In the newly developed kits, each involved β-lactam antibiotic conjugated with KLH was placed in the test line to compete for the gold chloride anti-β-lactams antibodies conjugate with the free beta-lactam antibiotics supposedly exceeding the permissib...

Saima Mehar1 and Salma Javed2*

...nd 72 hours in different concentrations of plant extracts. During in-vitro condition inhibition of egg hatchability and J2 juveniles’ mortality varied according to the concentration of plant extract. WC=1 showed 100% mortality after 48 hrs of exposure at 1% with the lethal concentration (LC50) values 0.168 mg/land all four concentration are 100 % t...

Amal Ramadan Fawy1, Hussein Yousef Ahmed2, El-Sayed S.E. Shabana3, Mohamed Abdelfattah Maky4*

...low-density lipoprotein) concentrations with greater level of HDL (high-density lipoprotein) concentrations. In conclusion, this study depicts for the quality status of the commercial beef burger in Egypt as well as the utilization of olive and rice bran oils as beef fat substitutes represents a promising technology for producing healthier burgers with enhanced quality characteristics.
 
Keywords...
Widiastuti Ardiansyah1,3*, Osfar Sjofjan2, Eko Widodo2, Suyadi Suyadi2, Danung Nur Adli2
...bacillus sp. bacteria in concentration of 1.4 x 1010 CFU/ml. A completely randomized design with six treatments and four replications was employed, with each experimental unit consisting of eight Mojosari ducks. The treatments consisted of T0 = basal diet, T1 = basal diet + 0.2% turmeric, T2 = basal diet + 0.8% turmeric, T3 = basal diet + 0.2% turmeric + 0.1% probiotics, T4 = basal diet + turmeric 0.8% + 0.6% probiotics, and T5 = basal diet + bacitracin 0.01%....

Xing Lu1, Mahmoud A.O. Dawood2, Fan Wu1, Hua Wen1, Wei Liu1, Juan Tian1, Ming Jiang1, Li-Juan Yu1, Xiang Li3, Ning Xu3 and Hong-Wei Liang1*

...educed triglyceride (TG) concentration (P < 0.05). The elevated expression of hepatic igf1 gene at the transcriptional level was positively correlated with dietary L-carnitine levels on the growth performance of Chinese soft-shelled turtle. Furthermore, dietary L-carnitine supplementation significantly up-regulated the mRNA levels of superoxide dismutase 1 (sod1) and lipoprotein lipase (lpl) genes in the liver of Chinese soft-shelled turtle (P < 0.05). A...

Yasmin M. M. Mahmoud* 

...ol one. In blood plasma, concentrations of total protein, albumin and globulin as well as activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were significantly (P<0.05) increased while, glucose, total cholesterol, uric acid and creatinine were significantly decreased (P<0.05) in G1 as compared to unsupplemented group. Kids in G1 group recorded the highest (P<0.05) concentrations of hemog...
Tariq Mehmood1, Muhammad Arif Khan1, Ayesha Safder Chaudhry1, Kamran Ashraf2, Muhammad Mohsin Ali1,3, Muhammad Faisal Ayoob3*, Mudassir Hussain4, Farrukh Saleem3 and Muhammad Fayaz3
...rence (P<0.05) in the concentration of sodium ions in group C compared to group A and B on day 15 and 30 of post-surgery. In contrast, the concentration of chloride, potassium, and bicarbonate ions was found to be insignificant. A significant difference (P<0.05) was reported in various blood parameters in group A and B when compared with group C. However, all the observed values were within the normal range. It was con...
C.A. Angel-Sahagún1, J.E. Ortega Palomares1, A.A. Hernández-Rangel1, C. Cruz-Vázquez2, R. Montesinos-Matias3, M. Valencia-Posadas1 and A.M. Cruz-Avalos4*
...xposed by immersion at a concentration of 1x108 conidia/ml, causing between 10-100% mycosis at ten days post-inoculation. Four isolates identified as Bb9, Bb6, Ma9 and Ma10, were the most pathogenic as mycosis reached 100%, which places them as potential candidates to be used as biological control agents.

...

Harpreet Kaur* and S.S. Hundal

...il spiked with different concentrations (0.3, 0.6 and 1.0 mg/kg dry soil) of imidacloprid under laboratory conditions. The activity of AChE and GST have been reported as potential biomarkers to assess toxicity levels, hence the activity of these enzymes were assessed to study imidacloprid toxicity. Inhibition of AChE activity was observed in both the earthworm species in all the doses of imidacloprid, indicating neurotoxicity. There was an initial increase in ...

Reyad R. Shawish, Reda E. Hamed*, Zakaria H. Elbayoumi 

...bab recorded the highest concentrations of PAHs and PAH4 (the sum of four different polycyclic aromatic hydrocarbons, named benzo[a]anthracene, chrysene, benzo[b]fluoranthene, and benzo[a]pyrene) made it of the highest health risk to the consumers, followed by kofta, shawarma and burger, respectively. Besides that, positive samples were compared with the European Commission Regulations (EC) of the maximum permissible PAHs limits in the meat products (≤...

Tooba Ather1, Muhammad Rafiq Shahid2*, Muhammad Ahsin Ayub3, Javed Iqbal2, Muhammad Asim Bhutta2, Muhammad Akram2, Naveeda Anjum4, Muhammad Rizwan6, Hafeezur Rehman5 and Umar Farooq5

...f host plants. Different concentrations of Pyriproxyfen and Buprofezin are Insect Growth Regulator (IGR) were bio-assayed against 3rd instar of CMBat Integrated Pest Management Laboratory, Department of Entomology, University of Agriculture, Faisalabad, during 2016. The results revealed that mortality of 3rd instar of P. solenopsis increased with increase in concentration of Pyriproxyfen and Buprofezin at three days of post ...

Muhammad Ajmal Khan1, Muhammad Yahya2*, Ali Hazrat2, Javed Khan3, Saeed Jan4, Tabinda Nowsheen2 and Inam Ullah5

...ydrate (DPPH) at various concentrations (50, 100, 150, 200, and 250 µL). The phytochemical analysis of methanolic leaves extract of T. camphoratum showed 2.80% alkaloids, 4.90% flavonoids and 1.80% terpenoids content. The antibacterial activity of the methanolic extract showed high effectiveness against Gram-positive bacteria (MRSA) with 23 mm zone of inhibition (ZOI), whereas against Gram- negative bacteria, S. typhi, E. coli and P. aeruginosa, with ZOI...

Ochieng Onunga George*, Owuor Ndonga Millicent Florence, Mario Kollenberg  

.... dysenteriae. Bacterial concentrations cfu/100ml of abattoir effluents ranged from 3.17 × 106 of TC. 3.94x104 of FC, 2.84x104 of E. faecalis, 8.65x104 of E. coli, and BOD of 828.04mg/l.Fungi isolated were., Aspergillus niger, Fusarium oxysporum, Penicillium spp, Aspergillus fumigatus, Saccharomyces cerevisiae, Aspergillus flavus. Parasites isolated from samples of effluent and water were Balantidium coli, Trichomonas hominis, Ancylostoma duodenale, and ...

Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6 

 

... the plasma progesterone concentration increased significantly (p<0.05) whereas estrogen, follicle-stimulating hormone and luteinizing hormone decreased significantly (p<0.05) in the non-vaccinated group compared to vaccinated and control groups in both seasons. The study outcomes shown that climate has neither significant effect on reproductive physiology nor exaggerated the effect on an experimentally infected animal. Furthermore, it is concluded that ...
Gamilat A. Elsaid1, Weam Mohamed Baher1*, Eman Shukry1, Abeer E. Abd El. Ghafar2, Marwa Shalaby1
...uce E. coli O26:H11 in a concentration-dependent manner. Therefore, strict hygienic measures should be adopted during all manufacture steps of kareish cheese. The use of KS and Bifidobacterium animalis subsp. lactis during kareish cheese manufacture is highly recommended.
 
Keywords | Kareish cheese, E. coli, Egypt, potassium sorbate, Bifido-bacterium
...

Hamdy Abdala Elnagar1, Wael Mohamed Wafa1*, Abdel-Khalek El-Sayed Abdel-Khalek2

... groups, serum P4 and E2 concentrations were in association with conception rate. In conclusion, the dual treatment of lactating cows on day 15 postpartum with 0.6 NAC/kg (orally for 7 days) plus 1% NAC (IU infusion for 3 days) may improve the reproductive efficiency, health performance, antioxidant enzymes, and inflammatory reaction during the postpartum period.
 
Keywords | Antioxidant, Cows, Cytokines, N-acetyl-cysteine, Reprodu...

Zahin Anjum1*, Farhat Shehzad1, Shaista Ali1, Sumbla Yousaf2, Amina Rahat1 and Hamid Ullah Shah3

...ton samples, the highest concentration of PAHs was recorded in mutton grilled on coals. The chrysene, naphthalene, flourenthene and DBAHA concentrations were assessed high in mutton samples grilled on coals. Furthermore, this research observed the factors that might be helpful in minimizing the level of meat contaminants if practiced with traditional grilling procedures.

...

Xiaoguang Su1,*, Yanjun Gao2, Yanling Wang1 and Yaohui Ma2

...d treated with different concentrations of HAE for 48 h. Cell viability was measured by MTT. Flow cytometry was used to detect the apoptosis rate. qRT-PCR and Western blot were used to detect the effects of HAE on miR-128-3p and EPHB2. The above method was used to detect cell viability and apoptosis rate after overexpression of miR-128-3p or inhibition of EPHB2. The dual luciferase report experiment verified the targeted binding relationship between miR-128-3p...

Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*

...follows: antigen-coating concentration, 0.6 μg·mL-1; serum dilution, 1:200; 60-min serum action time, 1:2000 working dilution of the enzyme-labeled secondary antibody; and 5% skimmed milk used as a blocking solution. No cross-reactivity was observed with the positive serum of Eperythrozoon, Trichinella spiralis (T. spiralis) and Hydatid cysts. The coefficients of the inter- and intraassay variations in the repeatability tests were lower than 10%. The...

Shambel Boki Dabi

...at downstream and lowest concentration (58±40mg.L-1) was found in the middle stream. The upper stream has a high CO2 concentration (14±3.6mg.L-1) while the middle stream has a low CO2 concentration (9.4±0.77mg.L-1). The maximum chloride content (28.7±2.6 mg. L-1) was found at downstream, whereas the lowest (22.7±4.1mg.L-1) was found in the upper stream. T...

Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3

...ere inspected of protein concentration. In the crude antigen, there were two bands with molecular weights of 73 and 64 KDa, compared to ten bands with molecular weights ranging from 209 to 24 KDa. The isolated fraction showed diagnostic properties of S. aureus mastitis using indirect ELISA with 100% sensitivity and 95 % specificity. The fraction validity lasted for more than one year of storage at -20 ºC. The current study introduces effective way for S. ...

Ahmed A. Mohammed1, Madeha H. A. Darwish1, Eman A.A. Negm2, Ayman S. Abdel- Maguid3 

...amp; H) fed with diverse concentrations of Probiotics. After the first week of treatment, the two groups (L and H) displayed a probiotics effect that manifested as a significant increase in body weight (BW) than the control group. After 28 days of treatment, the effect of high levels of probiotics (group H) demonstrated a significant increase of group (H) and either group (L) and control; meanwhile, both treated groups (L and H) exhibited a significant increas...
Idrissa Sacko1, Madou Dao1, Souleymane Sanogo1,2*, B. Michel Orounladji2, Kaly Diakité1, Diakaridia Traore3, Mamadou D. Coulibaly1, Amadou B. Cisse1
...een, respectively. Sperm concentration and total sperm count in ejaculate were determined by microscopy using single chamber Neubauer cell. Results show that ejaculate volume was influenced (p < 0.05) by individual (buck). Besides, individual and period of collection had no impact (p > 0.05) on mass motility. However, the period of collection significantly (p < 0.05) affects ejaculate volume, vitality, sperm concentration

Muhammad Akram1*, Muhammad Imtiaz Shafiq2, Amber Malik3, Farmanullah Khan4, Munir Ahmad Bhinder5 and Muhammad Sajjad6

...e endogenous glutathione concentration, superoxide dismutase, catalase, and glutathione S-transferase (GST). GST neutralizes reactive oxygen species to regulate physical homeostasis in the body. The target of this study was to evaluate the molecular role of GST genotypic polymorphism involved in the development of CVD. For this case-control study, a total of 504 participants including 261 CVD patients and 243 healthy individuals were enrolled after taking info...

Hakan Kececi1*, Yasin Ozturk2, M. Bahaeddin Dortbudak3, Seda Yakut4, Gurdal Dagoglu5 and Merve Ozturk1

...ATR). In this study, the concentration of ATR in cocklebur seeds was determined as 4 mg/g seed. The study involved 54 rats which were divided into a total of 9 groups including one control group. A single dose of cocklebur seed extract was provided by gavage after being concentrated by 2 ml for each animal (80 mg ATR/kg) except for those in the control group, and time-dependent hepatotoxic, nephrotoxic, hematological and biochemical effects were analyzed. No h...

Mehroze Amin1, Afifa Yaqub1, Qindeel Fatima1, Rabail Hassan Toor1,3 and Abdul Rauf Shakoori1,2*

...eters showed that higher concentrations of metformin were cytotoxic for the breast cancer cells with EC50 of 22.75µM. Analysis of expression of p53 and the interacting partner genes showed that metformin upregulated p53 expression in a dose-dependent manner, while the expression of interacting partners is downregulated indicating that metformin exhibits the anti-cancerous effect by regulating the p53 pathway. Altogether this study reports the effect of m...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
...assay showed that higher concentrations were lethal for the cancerous cells. IC50 was found to be 12.6 µM. Further contextualization of the therapeutic potential of G9a inhibition was provided by expression analysis of the G9a gene and genes associated with cell proliferation, tumor suppression, and apoptosis. The gene expression of fibroblast growth factor 1 (FGF1), enolase 2 (ENO2), cyclin D1 (CCND1), catalytic subunit of AMP-activated protein kinase (...

Muhammad Hassan Naseem1, Muhammad Mansha1*, Muhammad Triq1 and Peter C. Thomson2

...Significant increases in concentrations of MCHC, RDWc, PDWc and count with P=0.000 were observed in case subjects as compared to control subjects. Increased blood pressure (P=0.000), increased BMI (P=0.048) and decreased lung capacity (P=0.000) was studied in shisha smokers. Shisha smoking significantly increase the haematological indices, body mass index, systolic and diastolic blood pressure and reduces lung capacity.

...

Zaidi Zona, Zulfiqar Ali*, Rida Ahmad and Irfan Zainab

...tivity, DO, high nitrate concentration, and high salinity manifested to be the best habitat conditions downstream of Thakot area. To conserve the richness and integrity of fish communities, it is recommended that instream flow evaluations to include target species that occupy flow-sensitive habitat categories.

...

Muhammad Rizwan, Abu Bakar Muhammad Raza*, Muhammad Zeeshan Majeed and Muhammad Arshad

...nst D. mangiferae. Three concentrations (i.e. 6.25, 12.5 and 25.0%) of leaf extracts of neem, Azadirachta indica A. Juss (Meliaceae), eucalyptus Eucalyptus camaldulensis Dehnh. (Myrtaceae), datura Datura stramonium L. (Solanaceae), batho Chenopodium album L. (Amaranthaceae) and Indian lemongrass Cymbopogon citratus (DC.) Stapf (Poaceae) were tested against 3rd instar nymphs of mealybug. Results showed that mean mortality was higher (76.67%) after the applicati...

Mutassim Abdelrahman1, Ibrahim Alhidary1, Majdi Bahaddi1,2, Mohsen Alobre1,3, Riyadh Aljumaah1 and Rifat Ullah Khan4, *

...(P >0.05) between the concentration of all the elements in all lambs’ tissues, except for the concentration of aluminum in kidney and rumen fluid, as well as Mn in rumen fluid. Moreover, Mn concentration in rumen fluid was significantly (P < 0.05) decrease with zeolite supplementation, with significantly lower values for lambs from T2 when compared with T1 and control (339.47 v...

Hazem E. M. Hassanien1*, Awad M. M. Mahmoud2, Elsayed M. Abdel-Raouf1, Nabil M. Eweedah1 and Midhat. N. Nassif3

...nt of treatments -3 DCAD concentrations Tr1. (0.0, -100 or -180 mEq/kg DM) and -2 postpartum DCAD concentrations Tr2. (+250 or +350 mEq/kg DM). Prepartum urine pH was lower for cows fed -180 DCAD compared with -100 or 0.0 DCAD. Postpartum urine pH was higher for cows fed +350 mEq/kg compared with those fed +250 mEq/kg DCAD. Prepartum serum tCa, iCa, hydroxyproline (OH-PRO) was highest for -180 DCAD compared with both -100 an...
Muhammad Sajid Hamid Akash1*, Hina Sharif1, Kanwal Rehman2, Sumbal Rasheed1 and Shagufta Kamal3
...we also measured arsenic concentration in liver using HG-AAS technique. ATO exposed rats showed a significant elevation in serum glucose, reduction in serum insulin, rise in insulin resistance and alteration of carbohydrate metabolizing biomarkers when compared with unexposed rats. Arsenic concentration was found to be significantly high in liver tissues of ATO exposed rats. Contrarily, RSV was found to be effective in regul...

Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda 

...al motility, spermatozoa concentration, viability, and libido. Data were analyzed using the ANOVA test. The results showed that the volume and pH of semen did not differ in all treatments, but treatment A showed cream color and thick consistency over treatments B and C. The mass motility and sperm concentration of treatment C with the dose and period of GnRH administration resulted in the lowest values significantly differen...

Sajid Hanif1, Abdul Shakoor1,4*, Muhammad Farrukh Saleem1, Ifra Saleem2, Sajid Ali3, Muhammad Awais Ashraf4, Majid Nadeem4, Hira Shair4, Anwar ul Haq5, Rana Abdul Hamid Khan6 and Muhammad Amir Amin7

... spray, 10, 20 and 30-mM concentrations. Combined heat and drought stress increased unfertile tillers, unfilled grains and abortive, chalky and opaque kernels, along with declines in chlorophyll and relative leaf water contents (14% over control) the most than individual stress imposition. A 29.76% increment in yield was observed as comparison to to (control) by the use of exogenous proline and its quality attribute due to improved chlorophyll and relative lea...

Muhammad Umer Chattha1, Muhammad Ilyas2, Imran Khan1, Athar Mahmood1, Muhammad Bilal Chattha3*, Ambreen Fatima4, Muhammad Iqbal5, Muhammad Tahir Akbar6, Muhammad Mahmood Iqbal5, Faran Muhammad7, Muhammad Talha Aslam1 and Muhammad Umair Hassan1

...stress increased the Na+ concentration and the activities of SOD, POD and CAT. Moreover, Bhaker-2011 had maximum chlorophyll a, b, and activities of SOD, POD and CAT among the cultivars. In conclusion, Bhaker-2011 appeared as a salt tolerant cultivar that was linked with improved growth, photosynthetic performance and antioxidant activities.

...

Sri Suharyati, Siswanto, Madi Hartono, Kusuma Adhianto* 

...r weight, sperm count or concentration, sperm motility, and vitality. L-carnitine is an amino acid compound and is able to provide energy so that it can help improve sperm quality. The objectives of this research were to find out the sperm quality of Jawarandu goats after oral administration of vitamin E and L-carnitine. The research used 16 Jawarandu male goats, with a completely randomized design (CRD) using 4 treatments, control (P0), administration of vit...

Nagina Zeb* and Noor ul Amin

...ery treated with various concentrations of IBA (control, 2000, 4000, 6000 and 8000 ppm) at Ornamental Nursery, Horticulture Department, The University of Agriculture Peshawar. The trial was arranged in Randomized Complete Block Design (RCBD) with split-split plot arrangement. Planting dates were reserved in main plot, cutting types were taken in the sub plot and IBA concentrations were in sub-sub plot. The average results of...

Asim Faraz1*, Nasir Ali Tauqir2, Abdul Waheed1, Hafiz Muhammad Ishaq1, Syeda Maryam Hussain3, Riaz Hussain Mirza1, Rana Muhammad Bilal2, Muhammad Arslan Akbar4 and  Muhammad Shahid Nabeel5 

...ein, urea and creatinine concentrations were analyzed on haematology and biochemistry analyzer. The mean Hb concentration (P<0.05) was found to be 14.77±0.76 and 14.16±0.97 g/dl in non-breeding and breeding males, respectively. The hematological values of RBC, WBC and PCV were found to be varied (P<0.05) as higher in non-breeding animals, while the biochemical indices including cholesterol, triglycerides ...

Hassan A. Aidaros, Eman M. Hafez, Halla E.K. El Bahgy* 

...ive compound chosen, its concentration, and the cleanliness of the surfaces to which it is applied. The purpose of this study was to evaluate the effectiveness of some disinfectants (Prophyl 2000®, G7®, Pron-Tech®, Alkadox®, and Biodine®) at different concentrations and contact times at 20, 40, 60, and 90 minutes against field isolated serotypes of E. coli, Pasteurella multocida, Campylobacter jejuni, and...

Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1 

...ere exposed to different concentrations of garlic extract (0, 3, 6 and 9%, respectively) to evaluate the semen quality parameters after cooling and freezing process. Data obtained showed that, progressive motility, live spermatozoa and acrosome integrity % were higher (P < 0.05) in G1, G2 and G3 with reduced mortality percentage than control group after cooling process. In addition, a significantly increased (P < 0.05) of progressive motility, acrosome i...

Fatin Khalil*, Harinath Yapati, Zainab Al Blallam, Ronia Jose 

...n corpuscular hemoglobin concentration (MCHC) are both above the usual ranges of 25.6–31.8 fL and 14.8–45.9 g/dl, respectively. During the winter, dry ewes’ total protein and hemoglobin levels are significantly greater (P<0.05) than other types of sheep, according to a biochemical investigation. The results showed that the physiological, biochemical, hematology analysis and production performance of Naeemi sheep were affected by the season...

Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1* 

... the increase in C-Oo.Nc concentrations and exposure time. Analyzing the DNA damage in the oocysts exposed to different concentrations using comet assay revealed a significant direct variation (p ≤ .05) between the increase in the dose and the degree of the DNA genotoxic damage as represented by variations in the tail length (μm), percent of DNA in the tail segment, and tail moment (μm). Postmortem (PM) inspection o...

N. Wachida1*, P. M. Dawuda2,1, I. U. Ate3,1, P. I. Rekwot4, 2 

...er, and ovarian hormones concentrations. Twelve (n=12) non-pregnant, Zebu cows with 2-3 successive calving record were utilized. Temperature humidity index (THI) determined and rectal temperatures of cows were monitored. Cows were synchronized for oestrus using PGF2α. Follicular studies were carried out. Serum oestradiol and luteinizing hormones were monitored by Enzyme-Linked Immunosorbent Assay (ELISA). Blood samples for serum were collected for oestra...

Hurriya Alzahra1, Susmiati Susmiati2*, Sri Melia3 

...sign (CRD), with starter concentrations of 4 (A1), 5 (A2), and 6% (A3), as well as orange juice concentrations of 10 (B1), 15 (B2), and 20% (B3). The results showed that fermented milk with orange juice revealed antioxidant activity ranging from 25.04 to 37.71%, total phenol 38.32-67.20 mg GAE/gr, taste organoleptic test 3.20–4.08 (somewhat liked), aroma 3.26-3.76 (slightly liked), and texture 2.70–3.40 (rather l...

Hafiz Kashif Ali1, Iftikhar Ahmad1, Mujahid Ali2*, Zahoor Hussain3, Muhammad Ather Nadeem4, Malik Abdul Rehman5, Barkat Ali6, Muhammad Iftikhar7

...ax) with three different concentrations (1 ppm, 3 ppm, and 5 ppm) with the different number of sprays (1, 2, or 3 sprays). The first spray was applied after one month of transplanting, while the other two were applied at fortnight intervals after the first spray. Fisher’s Analysis of variance revealed that Isabion at 1 ppm as 1 spray, 3 ppm as 3 sprays, and 5 ppm as 3 sprays produced the highest plant height for three cultivars of stock. ‘Cheerful ...

Riaz Hussain1*, Adnan Ihsan2, Azaz Ali Shah2, Najeeb Ullah2, Hamza Iftikhar3 and Ranra Jalal4

... (Melia azedarach) @ 10% concentrations were used singly and tobacco dry leaves (N. tabacum) + Chinaberry dry fruits (M. azedarach) @ 1% + 5%, garlicbulb (A. sativum) + Red chili(Capsicum spp.) @1% + 2.5%, tobacco dry leaves (N. tabacum) + Red chili (Capsicum spp.) @ 1%+2.5%, and garlic bulb (A. sativum)+ Chinaberry dry fruits (M. azedarach) @ 1%+2.5% were used in combination as treatments. The results showed that Chinaberry dry fruits (M.azedarach) caused hig...

Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3 

...test, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration were determined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices...

Sajjad Khan, Fahad Karim Awan*, Zulfiqar Ali Gurmani, Muhammad Usama Hameed, Allah Bakhsh and Kainat Bibi

...nd salinity stress. Salt concentration of 80 mmolLˉ¹ showed non-significant effect on germination % and shoot length of both oat cultivars except salt concentration of 120 mmolLˉ¹, but tolerance in NARC, Oat is higher than PARC oat. Salt concentrations positively increased root length, except in PARC oat at 120 mmolLˉ¹. Drought stress (PEG) significantly reduced the shoot...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...perm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.

...

Khalid Hussain1, Muhammad Younas1*, Niaz Hussain1, Abdul Ghaffar1, Muhammad Raheel2, Anees Akhtar3, Muhammad Irshad1, Munir Abbas1 and Farah Shabir4

...eduction (75.16%) at the concentration of 3 g/liter of water followed by Derosal carbendazim) (65.76), Shinkar (59.44), Ridomil gold (52.41), Cabrio Top (44.17) and acrobat (41.86) respectively on comparison to control. Results are also helpful for the farmers for timely management of fusarium wilt.

...

Shad Mahfuz1,2†, Hong-Seok Mun1,3†, Muhammad Ammar Dilawar1, Keiven Mark B Ampode1,4, Veasna Chem1, Young-Hwa Kim5, Jong-Pil Moon6, Chul-Ju Yang1,7* 

...nia and hydrogen sulfide concentration were measured on weekly basis. There were no significant differences on the final body weight, average daily body weight gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR), between the incubators. However, ADG was numerically higher (P > 0.05) and FCR was lower (P > 0.05) in solar heating incubator compared with the control incubator. The consumption of electricity with solar based incubato...

Maham Tariq, Aqsa Akhtar and Nauman Khalid*

...mulation. Five different concentrations of fennel extract and carrot juice were added for the formulation of hard candy containing 30% (w/w) sugar, 30% (w/w) corn syrup, 0-40% (w/w) fennel seed extract, and 0 to 40% (w/w) carrot juice. The results showed the highest content of flavonoids in 40% (w/w) carrot juice formulation and the mixture of candies containing 10% (w/w) carrot juice and 30% (w/w) fennel extract, while the highest antioxidant activity was obs...

Zahin Anjum1*, Mubashra Tarana1, Shaista Ali1, Faryal Yousaf1, Amina Rahat1 and Sumbla Yousaf2

... gizzard had the highest concentration of Zn (24.84mg/kg), Pb (2.25mg/kg) and Ni (2.34mg/kg) while, had lowest concentration of Cu (3.30mg/kg) Ca (8.05mg/kg) Na (74.29mg/kg), P (130.67mg/kg) and Mg (15.85mg/kg). Liver had highest concentration of Cu (7.74mg/kg) and Mg (24.23mg/kg) while lowest concentration of Pb (1.24mg/kg) Ni (0.64mg/kg), K (205.20mg/k...

Sehrish Akram1, Abida Butt1* and Shakil Ahmad Khan2

...ive correlation with the concentration and negative with the age of larvae. From second to fifth instars, LC50 values of IMF ranges from 0.72 to 2.96, IMT from 0.86 to 2.75 and Pb from 1.93 to 6.88 mg/L. The toxicity of binary mixture of IMF + Pb showed synergism against all instars of silkworm. While, binary mixture of IMT + Pb displayed synergism, additive, and antagonism and antagonism activity in silkworm larvae in silkworm larvae. The results of our study...
Azra Kalhoro1, Abdul Aziz Mirani2, Fozia Khan Siyal1, Tahira Jatt2*, Abdul Razak Mahar1, Sadia Iram3 and Muhammad Abbas Bhutto4
...ased with decrease in SW concentration up to 25% SW. The minimum accumulation of the metal was noted with 100% FW (control). Among the five types of vegetables, cabbage and cauliflower revealed a high tendency of accumulating the metals. Hence, in order to avoid exposure of excess heavy metals to human health through vegetables, the cabbage and cauliflower crops may not be grown in the vicinity of Karachi city where the source of irrigation water is only sewag...

Ibrahim S. Abu-Alya1*, Nagwa I. Sheraiba2, Enas K. Aziz2, Noha A. Osman2, Afaf A. Kishta1 

...lasma urea and uric acid concentration. The results of gene expression showed that TNF-α and IL-1β genes expression was the highest in the fish supplemented with 1% ginger oil (T5). It is concluded that garlic and/or ginger oil supplementation to the fish diet enhances growth as well as immunostimulant agents and improves the general health of Nile tilapia.

Keywords | Gene expression, Physiology, Garlic oil, Ginger oil, Nile tilapia. 

Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2 

... semen volume, pH, sperm concentration, total sperm, total motile sperm, and frozen semen production of Aceh cattle (p < 0.05). However, it was found that there was no significant change in the sperm motility, before freezing, post-thawing, and during recovery across age (p > 0.05). Semen volume, total sperm, total motile sperm, and frozen semen production increased in bulls from 6 to 7 years of age and then gradually reduced from 7 to 10 years of age. I...

Sahar M. Saber1*, Ahmed M. A. Albehwar2, Amani M. Abas3, Omaima A.A. Alshamandy2, Noha A. El said4 and Marwa Y. Hamad4

...fully obtained with high concentrations by both cell cultures up to a dilution of 10 -5 yielding positive antiviral effect against RVFV, especially when inoculated one hour post virus infection or simultaneously with the virus. So, we could conclude that the prepared interferon has an antiviral activity against RVFV and further studies are needed to provide more understanding of the virus-host interaction and cellular antiviral response and to evaluate the pro...

Ayesha Saeed1*, Farzana Kishwar2, Ahsan Nazir3, Sharjeel Abid4, Muhammad Abiodullah5, and Danial Ali6

...racts were used at three concentration levels (0.5, 1, and 1.5 %). Scanning Electron Microscope (SEM) images confirmed the formation of optimized nanofibers with diameter range from ±49 to ±545 nm. It was observed that addition of leaf extract increased the solution conductivity and decrease the surface tension and viscosity. Chemical reactions and presence of functional groups of SA, PVA, HRS and MA were studied by Fourier Transform Infrared Spe...

Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1* 

... (P4), nitric oxide (NO) concentrations and histological evaluation through different stages of CL in buffaloes. Forty five paired samples of ovarian tissue from buffaloes were obtained from slaughterhouse. Ovaries were collected and CLs were classified morphologically into three stages: early, mid and late stages. Moreover, the CLs were frozen at -80 °C for NO estimation in CLs homogenate, RNA isolation and gene expression. Blood samples were obtained for...

Florence N. Nworah*, Nene O. Uchendu, Ifeoma F. Chukwuma*, Emmanuel C. Ossai and Emmanuel C. Ezeako

...lt;0.05) increase in the concentrations and activities of oxidative stress biomarkers. However, treatment with the blood of P. sinuatus reversed the effect of cyclophosphamide on the haematological parameters and the oxidative stress biomarkers. These results suggest that the P. sinuatus blood exhibits both haematopoietic and antioxidative potential and could be a good source for the amelioration of leucopenia and anaemia.

...

Aslı Çilingir Yeltekin

...buconazole exposure at a concentration of 2.5 mg/L. The obtained results indicated that caspase-3, 8-OHdG, and MDA levels significantly increased compared to the control (p < 0.05). The kidney and muscle tissues indicated a significant decrease in antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)) compared to the control (p< 0.05). Therefore, these data may reflect one of the molecular pathways...

Hasnain Raza1*, Tanveer-ul-Haq1, Muhammad Imran1, Nabeel Ahmad Ikram2 and Muhammad Bilal Shoukat1

...earch aims to assess the concentration of contaminants in wastewater. Samples of wastewater were collected from Wali Muhammad Canal, Multan, and analyzed for heavy metals (HMs) including cadmium (Cd), copper (Cu), nickle (Ni), and zinc (Zn), along with basic physicochemical parameters including biological oxygen demand (BOD), chemical oxygen demand (COD), pH, electrical conductivity (EC), total solids (TS), total suspended solids (TSS), total dissolved solids ...

Sanwal Masood1, Dildar Ahmed2, Silvia Machado1 and Sohaib Aslam1*

...showed the extremely low concentration of DO i.e. 1.6 mgL-1. All the heavy metals values were within the permissible limit as per world health organization (WHO) guidelines except for iron and silver. However, significant differences were present among the water samples of selected locations which indicated strong influence of type of land use on ground water quality. Monitoring of such activities by government departments is the deer need for conserving the q...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

...o;l/ mice) and different concentrations of sericin (0.03 mg/ml, 0.06 mg/ml and 0.09 mg/ml) and curcumin (1%, 2%, and 3%) were administered to the respective groups, then blood was collected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

...o;l/ mice) and different concentrations of sericin (0.03 mg/ml, 0.06 mg/ml and 0.09 mg/ml) and curcumin (1%, 2%, and 3%) were administered to the respective groups, then blood was collected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best

Hemat A. Abdel Magied*, Heba H. Habib, Amany H. Waly, Ahmed Fadl, Sayed M. Shalash 

... of produced meat as the concentrations of MDA and LDL were decreased and increased meat TP.

Keywords | Pectinase, Protease, Broiler, Low protein, Low energy. 

...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
...0%) volume (>4ml) and concentration (>0.5 billion/ml)] collected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was filled in 0.5 ml French straw at 4°C and kep...

K. H. El-Kholy1*, Enayat Hassan Abo El-Azayem2, Safaa Ataya Barakat2, Mona Gamel Mohamed3, Sara H. M. Hassab1 

... content. The caecal NH3 concentration significantly (P≤0.05) decreased when the dietary AV level increased. Haematological, plasma biochemical and antioxidant enzymes (SOD, GPx and CAT) were significantly elevated (P≤0.05) by the highest dietary AV inclusion (G4). The addition of AV significantly enhanced immune- responses; lymphocyte percentage, IgG and IgM increased when growing rabbits were fed G4 compared to the control group. In conclusion, dietary...

Maria Al Mazed1*, Md. Ashikur Rahman1 and Sk. Istiaque Ahmed2

...c exposure and excessive concentrations are also deleterious for the normal physiological functions of human. Consumption of fishes contaminated with toxic metals are neurotoxic and carcinogenic to blood, lungs, kidneys, bones, liver and other vital organs of human. The present review outlines the contamination of aquatic environment with heavy metals and their contagious effects on aquatic animals and their public health concerns.

...

Inam Ul Haq1*, Humara Umar1, Farah Umar2, Naeem Akhtar3 and Muhammad Jan4

...nt separate and combined concentrations of indole butyric acid (IBA), urea phosphate (UP) and paclobutrazol (PB). These chemicals were evaluated under seven treatments in controlled greenhouse environment and tested for different rooting parameters. The results obtained showed significant effect on rooting ability of the cuttings. The appealing results obtained at T6 (IBA+UP+PB) where maximum rooting 56.8% with average number of roots per cutting 4.4 and root ...

Muhammad A. Ahmad1, Shabbir Hussain2*, Humera A. Awan3, Muhammad Riaz4 and Muhammad Saeed1

... the effects of numerous concentrations of three PGRs (sodium-5-nitroguaiacolate, sodium ortho-nitrophenolate and sodium para-nitrophenolate) on the yield of wheat crop. Numerous compositions of PGRs were formulated and sprayed on the foliar parts of the crop during various growth stages of the crop. Control measurements were also performed involving water spray only under the identical conditions. The PGRs spray has resulted in a significant rise of leaf deve...

Sugiharto Sugiharto*, Ikania Agusetyaningsih, Endang Widiastuti, Hanny Indrat Wahyuni, Turrini Yudiarti, Tri Agus Sartono 

...meters, serum creatinine concentration was higher (P < 0.05) in HSD and HSD-P than in CONT and HSD-PC. In conclusion, germinated papaya seeds or a mixture of germinated papaya seeds and chitosan improved broiler production performance and kidney health while alleviating muscle protein catabolism in high-density pens from days 15 to 28.

Keywords | Broiler, Chitosan, Germinated seed, High stocking density, Stress 

...

Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2 

...act plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were motility, viability, abnormality, intact plasma membrane, acrosome status, DNA integrity, and movement patterns. Viability and abnormality parameters were stained with eosin-nigrosine, intact plasma membrane was stained with Hypo-osmotic (HOS) solution, acrosome status was stained with peanut agglutinin (PNA), and DNA integrity was stained with acridine orange (AO). The...

Reda Hassan*, Bahaa Abou-shehema, Sherif Zayed, Micheal Gorgy, Shama Morsy, El-Sayed Abu El-Hassan, Mahmoud El-Gbaly, Hanaa Basuony, Ebtehal Hassan 

...ed creatinine, uric acid concentrations, alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities and significantly (P<0.05) reduced the antibody titer against sheep red blood cells (SRBC), total protein, albumin values in broilers serum compared with ngative control. Aflatoxins supplementation significantly (P<0.05) increased malondialdehyde values in liver and significantly (P<0.05) diminished the reduced glutathione (GSH), glutat...

Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2 

...otal sperm output, sperm concentration, sperm mitochondrial potential, and initial semen fructose. All PEE levels increased serum follicle-stimulating hormone, luteinizing hormone, testosterone, immunoglobulin, and antibody titer, while reduced lysozyme. All PEE levels improved antioxidants capacity, while reduced malondialdehyde in blood serum and in seminal plasma. Advanced motility, membrane integrity, livability and sperm viability were improved, but sperm...

Nosheen Jehajo*, Nasreen Memon, Mansoor Ali Shah and Naheed Shah

...for each treatment. Four concentrations viz; (0.25, 0.50, 0.75 and 1%) each of the oil were prepared to observe the contact toxicity and surface protectant effect. Results showed that as percentage of concentrations increased mortality of adult beetles also increased with increased exposure time. In addition, among the dissimilar concentrations of oils, it was found that 0.50% of both oils...

Noor-us-Sabah1*, Mukkram Ali Tahir1, Tayyab Boota1, Muhammad Luqman2, Ghulam Sarwar1, Amir Aziz1 and Ameer Hamza1 

...vailable and extractable concentration of P (ppm) and K (ppm) in soil also increased. Although, all the organic sources had shown positive results but press mud application performed best regarding availability of K and poultry manure performed best in terms of P availability.

...

Hafiz Husnain Nawaz1*, Ayesha Ahsan2, Amir Afzal1, Muhammad Ashraf Sumrah1, Muhammad Jan1, Kashif Ali1, Muhammad Arsalan1 and Rizwan Latif3

... Thus, the 50µg/ml concentration plant extract solution has the lowest radical scavenging percentage, whereas the 1000µg/ml concentration plant extract solution has the highest DPPH percentage 80%. The results of the test reveal that the extract concentration had an influence on the assay’s absorbance, or, to put it another way, an increase in extract

Ahmed M. El-Waziry1,2*, Saeid M. Basmaeil1, Ibrahim A. Alhidary1, Gamaleldin M. Suliman1,3, Mutassim M. Abdelrahman1, Maged A. Al-Garadi1 

...d decreasing the ammonia concentration in the rumen. Moreover, ionophores increase propionic acid in the rumen, which is used as an energy source; hence, ionophores increase muscle growth by enhancing the daily weight gain due to the improved feed conversion rate. Studies indicate the effect of ionophores on the characteristics and quality of the carcass have been limited. Ionophoers may be enhanced the concentration of...

Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*

...lso directly reduced the concentration of free testosterone and estradiol in both sexes without any apparent effect on LH or FSH hormones. It increased the total cholesterol in males only and elevated the triacylglycerols and glucose levels in both sexes. The administration of testosterone to males, females, or both reduced the fertility index to zero. The mating success was also reduced from 80 to zero when both the males and females were administered with te...
Farah Abid1,2, Mohammad Saleem1,3*, Tahir Maqbool4, Tania Ahmed Shakoori5, Faheem Hadi4, Tahir Muhammad4, Saira Aftab4, Yasir Hassan7 and Shabana Akhtar4,6*
...d treated with different concentrations of E-AM (250, 500 or 750 mg/kg), and with standard treatment, piroxicam (10 mg/kg). Antioxidant assays (superoxide dismutase and catalase) and histopathological examination were performed to determine the values of various parameters of inflammation and arthritis. Real-Time PCR technique was applied to analyze the expression levels of different cytokines and interleukins involved in inflammation. Real-Time PCR results sh...

Saima Rubab1,3*, Ghazala H Rizwani2, Arjumand Iqbal Durrani3, Iram Liaqat4*, Urooj Zafar5, Mahjabeen6, Farah Batool7, Noor-E- Seher3, Naveera Younas3 and Ayesha Sadiqa8

... < 0.001) increase in concentration dependent manner, leaf extract displayed highly significant results even at low dose with better results at high dose compared with standard.

...

Edhy Sudjarwo, Muharlien Muharlien, Osfar Sjofjan, Danung Nur Adli, Dyah Lestari Yulianti* 

...nd carbon dioxide (CO2) concentration, and dust particle.. A total 5,051 heads laying hens used were Hy-line laying hens aged 28 weeks reared for 28 weeks on the experimental diet. A completely randomized design (CRD) with three treatments and twelve replications. The parameters observed was growth performance, microclimate, and health. The cage location consisted Z1: near inlet; Z2: middle of housing; Z3; near outlet. Result were analyses general linear mo...

Ghazala Nasreen1, Ali Hussain2, Komal Tayyab1, Muhammad Rashid3 and Sumaira Aslam1*

...la were fed on different concentrations of bioflocs bacteria enriched on banana peels) under different diet treatments for a period of 50 days. The diet treatments devised in this study were, T1 (bioflocs only with zero water exchange), T2 (bioflocs only with 10 % weekly water exchange), T3 (bioflocs + 50 % commercial diet and with zero water exchange) and T4 (bioflocs + 50 % commercial diet and with 10 % weekly water exchange). A control experiment C (commerc...

Nourhan M.M. Hassnin, Ali H.H. Ali and Hosny H. Kesba*

...nd esters with different concentrations. Terpenoids were the major compounds in root exudates of HR cultivar and fatty acids in MS ones. Such phytochemicals’ high ratios were proportionally connected with the host resistance reaction.

...

Adrial Adrial1*, Rudy Priyanto2, Salundik Salundik2, Ahmad Yani2, Luki Abdullah3 

...wed significantly higher concentration of Ca, P and Cu in blood serum if compared to Bali cows. However, the blood serum analysis of minerals suggested that there were Ca and Cu deficiency the beef cows raised in peatlands. In general, Ca and Cu deficiency in breeding cows raised in peatlands of Central Kalimatan Province had negative impact on reproductive performances including delayed age at puberty and first calving, delayed postpartum estrus and longer c...

Ibrahim Samir Abd El-Hamid1, Alaa Emara Rabee2, Moustafa Mohamed M. A. Ghandour2, Rasha Salah Mohammed3, Ahmed Mohamed. Sallam4 

...05) mean values of sperm concentration, mass motility, sperm motility, sperm viability, and cell membrane integrity. Plasma testosterone concentration and number of red blood cells were higher (P≤0.05) in both treated groups. However alanine aminotransferase, cholesterol, total antioxidant capacity and glutathione peroxidase levels were reduced (P≤0.05) in both treated groups. It can be noted that, supplementing of pla...

Hongfeng Guo

...ypoxia model: the oxygen concentration in the cabin of mice in intermittent hypoxia group was maintained at 40%, then decreased to 10% and maintained for 3 min. After that, it was continuously increased to 40%, and was decreased to hypoxia after 8 min. The operation was repeated 3 times every 3h. The drug administration group was treated with continuous CPAP during modeling. The expressions of malondialdehyde (MDA), superoxide dismutase (SOD) and prostaglandin...

Atilla Yıldız* and Ayse Kılıç

...ost-therapy, mean GSH-Px concentrations in group T was significantly higher (P<0.05) than that of group C. Likewise, mean erythrocyte GSH-Px concentrations raised significantly (P<0.05) in group T after resveratrol therapy compared to pre-therapy ones. In group T, there were significant differences between values before and after application in both, conceiving and non-conceiving cows. Resveratrol therapy improved conc...

S. F. A. Awad-Allah 1 and M. S. Khalil 2

...) was increased as their concentrations and exposure times were increased. The LC50 of VCT ranged from 30.86 × 103 to 83.77 × 105 mg/l, while the LC50 of Nemaless® ranged from 2.69 × 108 to 3.17 × 108 cell/ml. Results from the field experiments revealed that all of the tested treatments greatly suppressed (P ≤ 0.05) the numbers of galls, egg-masses and nematode final populations. The most potent materials in reducing the numbers ...

H. M. Hassan† and S. A. Ibrahim

...rt at the quarter lethal concentration (LC25) is effective in causing mortality of S. littoralis.

...

M. S. Bajestani1, K. Dolatabadi2†, E. Mahdikhani-Moghadam1

...ations @ 40%
concentration as compared to control.
...
A.M. El-Baha1, A. A. El-Sherbiny2†, M. Z. M. Salem1, N. Shaarawy3 and N. H. Mohamed3
...tivity at four different concentrations viz., 125, 250, 500 and 1000 mg/l against root-knot
nematode, Meloidogyne incognita under laboratory conditions. All concentrations significantly inhibited egg
hatching (31.24–66.35%) and mortality of second stage juvenile. Inhibition of egg hatching (%) and mortality (%) of
J2 were increased linearly with increasing co...

R. Singh1† and R. Z. Sayyed2

...trin (hydrolyzed starch) concentration in the infected regions as compared with normal healthy sections.
Protein localization found more pronounced at the sites of infection of M. incognita viz., cortical cells, giant cells,
abnormal xylem, medullary region and nematode body. Active RNA synthesis in infected cortical, medullary and
vascular regions was observed by positively dark stained nucleoli, nucleoplasm and cytoplasm o...

M. T. Javeed†, A. S. Al-Hazmi and Y. Y. Molan

...eased as the
concentrations of the culture filtrates increased. In general, T. harzianum (isolate No.27), T. hamatum (isolate
No.5) and T. viride (isolate No.8) were the most effective among all tested isolates. Therefore, these three isolates
were further evaluated in the greenhouse test on tomato against M. javanica. The three tested isolates inhibited
the reproduction, root galling and number of juveniles of M...

I. Kepenekci1†, S. Toksöz2, T. Atay1 and I. Saruhan2

...odes were applied at one concentration
(1000 IJs ml-1) (200 IJs insect-1 or approximately 15 IJs cm-2) at 25°C temperatures. The data for mortality was
recorded after 2, 4, 6 and 8 days intervals. Insect mortality of the adult was higher in all nematode treatments than
the control. Turkish EPN isolate S. carpocapsae (Black sea) and Kyrgyz EPN isolate S. feltiae (KG3) species
showed the highest mortality (98.6...

M. M. A. Youssef †and Wafaa M. A. El-Nagdi

...nter) at different yeast concentrations (1, 2 & 3% yeast) + a fixed concentration of 2% sucrose and three solutions of sucrose and yeast at different sucrose concentrations (2, 3 & 4% sucrose), a fixed concentration of 2% yeast were applied. The results indicated that there was a positive correlation (R= 0.097) between average percentage nematode...

A. S. A. Saad1, M. B. Al-Kadi1, A. A. A. Deeabes2 and A. M. El-Kholy2

...in vivo conditions. Five concentrations of all chemicals were prepared. Data of in vitro studies showed a marked nematicidal and nematode hatching inhibitory activity against root-knot nematode (M. incognita). However, the nematicidal activity differed between treatments as compared to control. Fosthiazate was found as the most toxic compound against J2 of M. incognita after 24 and 48 hour exposure time as well as inhibited the egg hatching significantly after...
A.A. Tanimola and B. Fawole1
...re tested at
concentrations of 50,000 mg/kg and 25,000 mg/kg in an experiment laid out in completely randomized design in the
laboratory. Data were collected on inhibition of egg-hatching, mortality of juveniles and analyzed using ANOVA (P
 0.05). Both concentrations of Aloe species extracts inhibited egg-hatching and second-stage juveniles mortality

C. Azhagumurugan† and M.K. Rajan

...es/plant) with different concentrations (5, 10, 15, 20 and 25 ppm). The control and treated plants
were analyzed for various growth characteristics such as, root and shoot length, fresh and dry weight of root and
shoot, leaf area, root gall index and chlorophyll content after 65 days. These growth characteristics found decreased
with increasing inoculum levels of egg-masses and increased with increasing

R.R. Arain, R.N. Syed, A.Q. Rajput, M.A. Khanzada1, N.A. Rajput and A.M. Lodhi1

...icantly. Three different concentrations of neem extract, furadan and Trichoderma harzianum
were evaluated to control the M. incognita. All concentrations proved effective in reducing the disease
development in nematode inoculated tomato plants. However, high dose of furadan became phytotoxic and plant
growth was checked together with a number of galls. While, higher doses of neem extract a...
C. Azhagumurugan and M.K. Rajan
...f M. elengi at different concentrations (5, 10, 15, 20 and 25
ppm). The control and experimental plants were analyzed for various biochemical constituents such as,
carbohydrate, protein, amino acid, lipid, proline and phenol content after 65 days of treatment. Carbohydrate and
protein were found decreased with increasing inoculum levels of egg-masses and increased with increasing
concentr...

S.A. Khan†, M. Abid and F. Hussain1

...d methanol extract @ 10% concentration after 72 hours exposure time.
Similarities between Ward’s cluster analysis of hatching egg and larval mortality in water and methanol extract of
different seaweeds showed significant difference. Methanol extract was more effective as compared to water extract.
In conclusion, use of seaweeds is positive sign against harmful microorganisms which are responsible for
consi...

N. Hajra

...d plants. Higher proline concentrations
reflected in leaves of treated plants as compared with mycorrhizal treated plants. The decrease in shoot length,
number of leaves and leaf area were observed in non mycorrhizal and plant treated with other biotic and
abiotic stresses. Higher amount of proline was produced in non mycorrhizal and stressed plants in result of
reduced availability of water and dry matter transl...

I. Umar† and A. Mamman*

...were treated to the same concentrations of the extract in Petri
dishes containing 1000 second stage juveniles of M. javanica. Egg hatch inhibition and larval mortality was
observed over a period of 72 hrs. Sterilized loamy soil (4 kg) contained in 36 perforated plastic pots (30 cm dia.
and 40 cm depth) were separately mixed with 0, 30, 40, 50 and 60 g (equivalent to 0, 10 15, 20 and 25 tons/ha) of
the fruit powde...

O. A. Fabiyi

... at very low
concentrations compared with standard carbofuran. Therefore, the leaves of D. oliveri could be employed as a
viable source of natural nematicide instead of synthetic toxic nematicides.
...

T.S. Rizvi and F. Shahina†

...lity after 72 hrs at all concentrations as compared with standard carbofuran. Whereas, HE-CC and HWCC
showed 100% mortality only at highest concentrations after 72 hrs. Ethyl acetate fraction was most active at
highest concentrations causing 60% nematode mortality after 72 hrs. However, aqueous fraction (1 and 0.5)
showed 40% mortality after 72 hrs expos...

M.M.A. Youssef and A.M.S. Lashein†

...esistance inducer at the concentrations of 5, 10 and 20
mM against nematodes and consequently on date palm yield. The results showed that number of R. reniformis in soil
and roots and Helicotylenchus sp., were reduced in soil one month after application at the harvest according to the
tested concentrations as compared with control significantly (p≤0.05). GABA achieved at the highest and...

A.A. Mokbel

...son with two
concentrations of the three mixed Pp isolates caused the highest reduction of 74.8-86.9% in number of nematode
root galls, egg-masses/root system and number of J2/250 cm3 soil compared with any single Pp isolate application.
Meanwhile, all treatments in 2nd season with both concentrations of all Pp isolates either alone or as a mixed isolates
resulted in 65.7-94.7%...

A. Khan†, Saifullah, M. Iqbal* and S. Hussain

...pecies at four different concentrations and three time intervals were tested against the
nematodes. Nematodes killed by the application of crude extracts of Pleurotus species never recovered to life after
placing in simple water. P. citronopileatus was found more effective than other species and it killed 100%
nematodes after 24 hrs followed by fruiting body extracts of P. florida 99% and waste of P. ostreatus 77%.
...

W.M.A. El-Nagdi†, M.M.A. Youssef and M.G. Dawood*

... with distilled water at concentrations of 2.5, 1.25 and 0.625% and drenched in each plot. Results showed that
the plant extracts showed nematicidal and nematode-hatching inhibitory activity reduced nematode criteria including
number of galls, egg-masses and hatched juveniles on roots of eggplant and number of juveniles in soil at harvest
stage compared to untreated plants significantly (p ≤ 0.05). Lower

Jax Vincent Gamulo, Maye Pearl Bolina, Jessica Serena Brion, Via Crishiela Nicole Dela Rosa, Roxanne Francesca Maglaya, Carl Lexter Tan, Aimee Caye Chang* 

...etween the plant extract concentration and the mortality time (R2 = 0.37). Meta-analysis revealed a significant summary effect among included studies, with high statistical heterogeneity (P < 0.05). Moreover, the forest plot showed significant difference (P < 0.05) between the activity of plant extract and comparator drugs. Egger’s test revealed that this SR-MA has a low risk of publication bias (P < 0.05). Overall, the results confirm that trop...

Lulu Ding, Ke Wang, Ruxue Huang, Wenjing Yu, Bingzhao Yan, Mengli Jiang and Jicang Wang*

...l count (RBC), hemoglobinconcentration (HGB), and hematocrit(HCT) of serum were significantly reduced after Cd exposure for 4 weeks. Cd increased the expression of GSH and MDA and decreased the activities of CAT and T-SOD. Furthermore, in the Cd-treated rats, the glomerulus contracted, the renal tubular structure was damaged, the epithelial cells were disordered and degenerated, and a large area of lesions occurred. However, treatment with Nar slowed down the ...

Mengxue Sun1, Yanjiao Li1,2, Zhen Gao1, Qinghua Qiu1,2*, Xianghui Zhao1,2, Lanjiao Xu1,2, Huan Liang1,2, Ke Pan, Mingren Qu and Kehui Ouyang1,2*

...acidosis mitigation. Low-concentration niacin could relieve subacute ruminal acidosis, and it could be added after symptoms appear. However, niacin had a negative role in the protection of acute ruminal acidosis.

...

Ronny A.V. Tuturoong1*, Sony A. E. Moningkey1 and Nova L.I.M. Ogi2

...igestibility (NDFD), NH3 concentration and efficiency of microbial protein synthesis (ESPM) were found in T2 group (64.04%, 65.05%, 60.63%, 189.36 mg.L-1, and 24.10 gr N.kg-1. BOTR-1, respectively). While the highest ADF was found in the T3 group (56.37%). The study suggested that T2 group produce the highest nutritional value compared to other combinations. The high level of the protein content of corn stover was proven to increase microbial protein synthesis...

Salman Hussain1, Basit Zeshan2*, Rabiya Arshad1, Saba Kabir1 and Naveed Ahmed3

...mycin minimum inhibitory concentration (MIC) E-test. The MRSA isolates were detected for the presence of mecA, mecC, and nuc genes. Congo red agar (CRA) method was used to assess the ability of isolates to form biofilms. The results of the study showed that the prevalence of MRSA was 48%. The MRSA isolates were highly resistant (100%) to penicillin, β lactamase inhibitors, cephalosporins, and macrolides. All the MRSA isolates were susceptible to vancomyci...

Kalsoom1, Nasir Shah2, Muhammad Ibrahim3*, Tahira Bibi1, Kazim Ali4 and Zahir Shah2

...d varieties were dead at concentrations more than 50 mM of NaCl in in vitro condition (75, 100 mM). Variation was observed in the level of tolerance to salinity in these potato varieties. Increasing NaCl (mM) level reduced the overall plant growth, number of shoots, number of nodes and leaves, root and shoot lengths (cm), fresh as well as dry root and shoot weights (g). In contrast, proline, Na+ and K+ (mg g-1 FW) contents increased, while total chlorophyll (m...

Saima Mumtaz1,3*, M. Imran Kasana1, Riaz Alam1, Noorullah Khan1, Muhammad Noman1, Sanjeela Sabahat1 and Hussain Shah2

...cantly influenced by IBA concentrations, litchi cultivars and their interaction at p<0.01. The interaction of 5000ppm IBA with “Gola” was found appropriate for development of heavier (10.2±0.57gm), longer (27cm) and more number of roots (6.4±0.81) compared to all other cultivars. Moreover, considerable difference was observed for root ability among all litchi cultivars tested as “Gola” produced maximum, longer and heavi...

Dina N. Ali1*, Sayed H. Elhabtey2*, Manal M. Amin1  

.... The minimum inhibitory concentration (MIC) of MO and Nano-MO was 3% and 2%, on Listerea monocytogenes, Staphylococcus aureus, Salmonella typhi and E. coli bacteria, respectively. While, in inoculation of MO and Nano-MO in cheese complete reduction of examined bacteria was recorded at 3rd week of storage except for L. monocytogenes Nano-MO killed it at 2nd week. Overall acceptability (OAA) investigations showed better results of Nano-MO than MO. Nano-MO showe...

Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4 

... TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplementations were sequentially incubated for 15 minutes at 37 ºC and cooled at 5 ºC for 2 hour than packed in 0.25 ml straws, and cryopreserved in liquid nitrogen for 24 hours. Consequently, straws were thawed at 37C0 for 30 seconds and motility was accessed with the help of computer-assisted semen analyzer (CASA) and membrane integrity with...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...< 0.05) augmented the concentration of total protein, triglycerides, cholesterol, and calcium in the plasma. Furthermore, a linear increasing effect (p < 0.05) on the T3 and E2 hormone concentrations and on the humoral and cellular immune response were obtained as the dietary BSFL level increased. Moreover, BSFL treatment linearly increased the profit margin, the cost benefit ratio and the return on investment per bird...

Bashir Ahmad1*, Ali Muhammad Yousafzai2, Waqar Ali1, Ikram Ilahi1, Farman Ullah3, Saeed Ahmad1, Ayaz Ali Khan1, Umair Ahmad2 and Hafsa Maria2

...telets, haemoglobin, MCH concentration, and HCT levels did not significantly alter after treatment with garlic aqueous extract and silymarine. However, a substantial (P 0.05) rise in the overall WBC count was seen. The current research findings revealed that silymarine, a common drug for hepatoprotection, might be replaced by garlic aqueous extract as a herbal remedy. However, more research is needed to identify the bioactive ingredient and determine the effec...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Mohammad A. Al-Mahaish4, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel5
... and cholesterol profile concentrations in the serum were significantly (p < 0.05) improved by increasing the level of MWM into broiler diets up to 6%. In contrast, some traits of carcass composition and meat quality as well as physiological parameters deteriorated when adding higher levels of the MWM (8-10%) to the diets, compared to the control. Economically, there was a linear and quadratic (p < 0.05) decrease in the protein cost of the diet and a lin...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
...iability, abnormalities, concentration, total motile spermatozoa, chromatin damage, intact acrosome hood of spermatozoa, and sperm capacitation. The treatments consisted of T1 = unsexed semen, T2 = sexed semen X-bearing sperm, and T3 = sexed semen Y-bearing sperm. A general linear model with three treatments and ten replications was performed in this research. The data were analyzed using a general linear model using SAS Demand on Academics, and the least sign...

Muhammad Adeel Qureshi1*, Raza Ahmad2, Bilal Ahmed1, Tanzim Ullah Khan1 and Muhammad Yasin3

... i.e. CIM1 and CIM2 with concentrations of 2, 4-D 2mg/lit and 3 mg/lit respectively. Among the two different callus induction media (CIM), CIM1 with 2mg/lit auxin (2, 4-D) showed the best results. All the internodal explants placed on CIM1 produced 100% callus while CIM2 produced 70% callus. No callus was observed from leaves on CIM1 and CIM2. After callus induction, four different types of media were used for plant regeneration, having different

Ghulam Sarwar Shah1,2, Maqsood Anwar Rustamani1, Rab Dino Khuhro1, Rehana Naz Syed1 and Abdul Mubeen Lodhi1

...ides’ main effect, concentration’s main effect, and fungicides’ × concentration’s effect are highly significant. Generally, based on their effectiveness, all tested fungicides were divided into three groups i.e., highly effective, moderately effective, and completely ineffective. All isolates of P. aphanidermatum were grown at par with the control when exposed to different ...

J. Salma and F. Shahina

...o culture at the highest concentration the production of infective juveniles were 60 × 10 4 to 87.4 × 10 4 IJs from each larva of G. mellonella 4.2 ×...

A. N. Mahar, N. D. Jan†, A. Q. Mahar and S. R. Gowen

...; C. When different dose concentration levels were tested in the sand arena, all dose concentrations resulted satisfactory mortality but a high level of dose concentration (400 IJs / ml) caused maximum insect mortality in all isolates. A similar response was observed in infectivity test when maximum percentage of IJs of both isolates of 

A.E. Ismail and M. M. Mohamed

...ns between the evaluated concentrations and the obtained reduction in numbers of the nematode stages and also all plant growth parameters including root weight (yield) and total soluble sugars.

...

B. Archana and R. Saxena

...g French bean. Different concentrations viz., 10000 ppm (S), 5000 ppm ( S/2), 2500 ppm (S/4), 1250 ppm(S/8), 625 ppm (S/16) and 312.5 ppm (S/32) of aqueous extract of O. sanctum roots was applied by seed soaking and foliar spray methods.

...

O. A. Fabiyi, G. A. Olatunji and O. Atolani

... juvenile mortality. 75% concentrations of fraction being more active and showed significant differences from other concentrations. Fractions from A. boonei

Mohamed S. Abbas1*, Adel E.M. Mahmoud2, Hemat S. Mohamed3, Adam Cieślak4 and Małgorzata Szumacher-Strabel4

... and forbs) contents and concentration on ruminal fermentation, pH, ammonia (NH3), total gas production (TGP), methane and volatile fatty acids (VFA) concentration. Twenty-six wild palatable forage plants were identified and analyzed for pH, NH3, TGP, methane and VFA. The results indicated that in grasses the highest pH and NH3 concentration was found in Aeluropus lagopoides, TGP in Ammoph...

Shah Murad Khan1, Hamayun Khan1, Younas Muhammad1, Haq Aman Ullah1*, Muhammad Tariq Tunio2, Ali Gohar2 and Fazli Rabbani

... lower progesterone (P4) concentrations for MSS than POP group. Cows with higher E2 and lower P4 levels at estrus got conceived (P<0.05). Highest level of E2 was recorded in pregnant cows in POP group in comparison with MSS. The P4 concentration of pregnant cows in MSS was lowest at the time of insemination (P<0.05). Induction of fertile estrous was maximum in POP group than MSS, but conception rate in cows receiving T...

Hesham A. Mahran1,2*, Shawky M Aboelhadid3, Saad S. Alqahtani4,5, Mohamed Z. Sayed Ahmed4,5

...rts sesame oil. Multiple concentrations from these formulations were prepared and tested against laboratory-reared adults and pupae of C. pipiens. The safety results showed that the ordinary form of cinnamon oil was safer for human skin cell fibroblast and human epidermoid Skin carcinoma than the other forms at high concentrations. Meanwhile, CNE was potent antibacterial activity even at the lowest c...

Asmaa A. Darwish*, Mona A. Mahmoud, Adel M. El-Kattan 

...ytes, and trace elements concentrations and these changes were more prominent in goat than in sheep. While, the diseased ewes suffered from higher degrees of leukocytosis, total hyperproteinemia, hypoglycemia, increased kidney function tests, and oxidative stress than the diseased does. Total antioxidant capacity (TAC) and Zn had the highest likelihood ratios among the suggested markers with acceptable sensitivity, specificity, PPV, NPV, and accuracy rates, an...

Van-Thanh Vo1, Thi-Hieu Tran1, Thi-Truc-Thao Nguyen1, Van-Tri Truong1, Cu-Thien Pham1, Thanh-Minh Pham2 and Huyen Nguyen Thi Thuong1*

...ilapia with an infective concentration of 106 CFU/mL. The experiment was arranged with 2 control treatments: control 1 – commercial feed + no infection; control 2 - commercial feed + infection of S. agalactiae; and 3 experimental treatments with essential oils concentrations of 0.125%; 0.25% and 0.5%. The study examined the blood cell morphology and physiological blood indices of red tilapia (including hemoglobin; hema...
Hafiz Abdullah Shakir1*, Iqra Javed1, Muhammad Irfan2*, Shaukat Ali3, Muhammad Khan1, Farah Rauf Shakoori1, Javed Iqbal Qazi1 and Muhammad Abrar Yousaf1
...n and 0.45% of substrate concentration showed highest tannase activity. The results depict utilization potential of low cost substrate (corn leaves) to reduce the production cost of tannase.

...

Songruo Tao1, Cuiyi Liao1, Jinju Peng1, Yuexia Ding1,* and Yi Ma1,2,*

...bacteria. Five different concentrations of florfenicol (0 mg·kg-1, 0.1 mg·kg-1, 1 mg·kg-1, 10 mg·kg-1 and 100 mg·kg-1) were used to collect soil samples on the 7 d, 21 d and 49 d after dosing. The effects of florfenicol on 16S rDNA sequence diversity of soil phosphorus-solubilizing bacteria were determined by amplifed ribosomal DNA restriction analysis (ARDRA) and enterobacterial repetitive intergenic consensus-polymerase cha...

Saleem Hussain1, Muhammad Tayyab1, Tauqir Anwar1, Talha Nazir2*, Muhammad Zeeshan Majeed3, Zohaib Asad2,4, Muhammad Adnan3 and Tajwar Alam5

...ted with three different concentrations (i.e. 250, 500 and 1000 ppm) of methanolic plant extracts under controlled laboratory conditions, while three different concentrations (25, 50 and 75 g/L) of each botanical extract was tested in the field trial. Results showed a significant mortality and suppression of S. graminum individuals at 72 h after treatment in the laboratory and at 10th day post-treatment in the field. In labo...

MARIUM ASLAM, MUHAMMAD JAVED, FAIZA AMBREEN* & FARIHA LATIF

...atla catla. To check the concentration based effect, C. catla were exposed, separately, to 96-hr LC50 of zinc and its sub-lethal concentrations viz. 2/3rd, 1/4th and 1/5th of LC50 for 30 days at constant laboratory conditions. After 30-day, the fish were sacrificed and their liver and kidney were analyzed for peroxidase enzyme activity. Change in activity of peroxidase enzyme in the fish exposed to sub-lethal

Chun Ik Lim, Hyeon Kwon Kim, Kang Nyeong Heo, Are Sun You, Hyo Jun Choo* 

... blood parameters, serum concentration of albumin (ALB) was significantly (P < 0.05) higher in the group fed diets supplemented with FGP than in the CON group fed a basal diet. FGP supplementation quadratically (P < 0.05) increased serum total protein (TP) level. Aspartate amino transferase (AST), cholesterol (CHOL), and triglyceride (TG) concentrations were significantly (P < 0.05) decreased in the FGP2.0 group tha...
 
 MUHAMMAD MUNEEB SOHAIL1, FATIMA AMEER2, GUR CHARN SINGH2, MUHAMMAD ZAID1* 
...yslipidemia involves low concentrations of high-density lipoprotein cholesterol (HDL) but high concentrations of other plasma lipids. This study reanalyzes the relation between plasma lipids and various anthropometric parameters. In this cross-sectional type of study, the participants (n=53) were recruited for the study. Participants were given one-day free health camps and they were anthropometrically measured. Plasma lipid...
 
 MUHAMMAD WAHEED MUSHTAQ1*, NAUMAN JAMIL KHAN1, NAVEED ASLAM DOGAR1, AISHA HAMEED2, ABDUL HAMEED2, MALIK ADIL2, HAROON IFTHIKHAR3 & SAIRA ISHAQ
...fast method to determine concentration of vitamin C (ascorbic acid) from leafy vegetables. So, concentration of Vit. C was measured spectrophotometrically by making a red color complex between 1, 10 Phenanthroline and Vit. C in the presence of ferric chloride (FeCl3). This colored p...

Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3

...t and minimum inhibitory concentration was calculated. HepG2 cell were transfected stably to express nonstructural proteins NS3 and NS5A of HCV 3a genotype. Western blot and RT PCR confirmed stable protein expression. Immunofluorescence assay was done to check subcellular localization of these nonstructural proteins. The CC50 value for A. indica seed extract for HepG2 cells was 650 μg/mL. The extract concentration 500 &mu...

Doaa H. Assar1, Rasha A. Al-Wakeel2, Zizy I. Elbialy3, Mahmoud M. El-Maghraby4, Helmy K. Zaghlool5, Adel A. El-Badawy4, Abdel-Khalek E. Abdel-Khalek6*

...d abnormality as well as concentration and output of sperm cells were significantly by SPL. In serum and seminal plasma, glutathione, superoxide dismutase, catalase, and glutathione peroxidase significantly increased, while malondialdehyde significantly decreased in SPL groups. Based on our results, spirulina as a dietary feed additive (5 g/kg diet) could be used safely for improving growth, health, antioxidant defense system, and semen quality of growing cros...

Yusmarini Yusuf*, Usman Pato*, Shanti Fitriani, Emma Riftyan, Evy Rossi, Diky Arma Fauzi, Ghina Ismadiah, Miftahul Hidayah, Windy Sabiliani

...ervatives with different concentrations, namely without the addition of preservatives (P1), the addition of 0.3% sodium nitrite (P2), 2.5% chitosan (P3), and 0.6% bacteriocin (P4). All samples were stored at freezing temperatures for 30 days. Data were analyzed statistically using analysis of variance, and differences between treatments were analyzed using DNMRT at the 5% level. The results showed that the application of various preservatives might impact the ...

Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*

..., the minimal inhibitory concentration (MIC) for amikacin and norfloxacin is significantly reduced from 64 µg/mL to 1 µg/mL and from 256 µg/mL to 8µg/mL respectively with frictional inhibitory concentration (FIC) index 0.25. Therefore, the FIC index recognized a synergistic activity between amikacin and norfloxacin against all P. areuginosa isolates. The mPCR was an efficient tool for detection of vir...

Fathin Faahimaah Abdul Hamid1, Mohd Farhan Hanif Reduan1*, Jasni Sabri1 , Faez Firdaus Jesse Abdullah2,Mohammed Naji Odhah1, Nur Athirah Binti Abdul Manaf1, Mohd Jefri Norsidin2 , Siti Nor Che Yahya1, Intan Noor Aina Kamaruzaman1, Nur Zul Izzati Mohd Rajdi1 

...nnheimia haemolytica 107 concentration was inoculated intranasally to all goats except goats of Group 1 which served as the negative control, Group 2 was the positive control, Group 3 goats treated with oxytetracycline, Group 4 goats were treated with flunixin meglumine, and group 5 received both oxytetracycline and flunixin meglumine treatments. Blood samples were collected at 24 hours, days 5, 7, 9, 11, 14, 21, and 28 post-inoculation for the clinical pathol...

Anhar Ibrahim Elhanafy*, Amr Mohamed Mousa, Amaal Mohamed Kamal 

...doe) represent different concentrations of BV, while the fourth group was control. Twice weekly injections of does were carried out along 12-weeks. Results revealed that BV treated does were significantly improved RBCs count, WBCs, serum total protein, albumin and globulin concentrations, as compared to control. Upon treatment, a significant decline in aspartate aminotransferase, alanine aminotransferase, serum total biliru...

Hussein Jabar Jasim1*, Amer Murhum Al-Amery2

...evealed that the various concentrations of Z. officinale methanolic extract used in our study were highly sensitive to it and that their activities differed noticeably from those of the positive (albendazole) and negative (chlorine-free water) controls. The ovacidal influence of ginger extract at a dosage of 5mg/ml with 24, 48, and 72 hr. treatment times was 90.1, 95.8, and 99.2% respectively, compared to the positive and negative control groups, which had ova...

Ahmed E.A. Mahgoob1, Wafaa M.A. El-Nagdi2, Mahmoud M.A. Youssef2*, Entesar H. Taha1, Mona M.S. Zayed3, Hassan Abd- El-Khair4 and Nora R.A. Saleh2

...acterial isolates at two concentrations (25 and 50%) significantly (p≤0.05) inhibited M. incognita- egg hatching at each exposure period (24, 48, 72 and 96 hrs) compared to untreated control. As for the percentages of the second stage juveniles mortality increased with increasing the concentrations and exposure periods. Generally, the highest egg hatching inhibition and juvenile mortality occurred after 96 and 72hrs expos...

 Salwa N. Zeinl and Hanaa S. Zawam2

...ic titer of l: 8000. The concentration of IgG and IgG conjugate with alkaline phosphatase was l: 1000. The prepared antiserum was used for detection Of ACLSV using ELISA dot immunobinding assay (DIBA). The presence of the virus was checked in blossom parts. (Petioles, stigma, and flower cup).

...

Muhammad Afzal Naeem1*, Muhammad Hassaan Khan2, Tahmina Nazish3, Uzma Bashir4 and Midrarullah5

...oot, stem and leaves) at concentration (0-100% v/v). Among the various test extracts of G. hirsutum, leaf extract were found to impart maximum inhibitory effect on the germination indices of the wheat (T. aestivum) followed by the stem extracts, as the highest concentration of ACWE (i.e. 100%) caused the reduction of 45.65, 54.55, 50.9, 61.6, 5.3 and 9.4 % in germination percentage, germination energy, germination index, vig...

Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago 

...ariations in spermatozoa concentrations. Semen from 12 KBC was collected and evaluated to determine their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into 500µl of Ringer’s lactate solution with various concentrations of spermatozoa, namely 50×106, 100×106, and 150×106. Fertile period and ...

Muhammad Ather Nadeem1, Bilal Ahmad Khan1,2*, Neelam Yaqoob3*, Abdullah2, Amina Iftikhar4, Muhammad Irfan5, Hafiza Zara Saeed6, Syed Saqlain Hussain7 and Sidra Shakil8

...void variance. Different concentrations of aqueous extract of wild radish (0.0 %, 3.0 %, 6%, 9.0% and 12.0 %) were applied on ten seed of pea. It was observed that maximum emergence percentage (94.25 %), shoot length (5.52 cm), root length (6.20 cm), fresh biomass (0.95 g), dry biomass (0.32 g), minimum time to start emergence (2.56 days), mean emergence time (4.51 days), time to complete 50% emergence (3.62 days), was observed under control while minimum emer...
Saiwa N. Zein
...c titer of I: 10000. The concentration of 12G and lgG conjugated with alkaline phosphatase was I : 1000. The prepared antiserum was used for detection of T RV using dot-blot immunobinding assay (DBIA). This is the first report for isolation of Tobacco rattle virus Tohrarirus (T R V) from D.Kaki under the Egyptian conditions.
...

Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3 

...The total immunoglobulin concentration (g/dl) measured four weeks post immunization was significantly higher in the serum and milk whey of immunized cows as compared to the non-immunized cows (P<0.01). The highest ELISA titers against E. coli (O111), S. aureus, Str. agalactiae and Str. dysgalactiae were recorded 4 weeks post immunization in the serum and milk whey of immunized cows as compared to the non-immunized group. In cows suffering from subclinical m...

Saad Zaghlou El-Damrawy1, Reda Ali Hassan2*, Ahmed Maher Bakhaty1, Adel M. Nasr2, El-Sayed A. Abu El-Hassan2  

...count (TLC), haemoglobin concentration (Hb), haematocrit levels (HCT), mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC), as well as lymphocyte %, neutrophil %, and monocyte %. All the indicators, including feed intake, body weight gain, haematological tests, and serum biochemical tests, were significantly (p<0.05) enhanced by both liver tonics. The st...
Mansoor Ayoob1, Atta Hussain Shah1, Zaheer Ahmed Nizamani2, Muhammad Faisal Ayoob2,3*, Deepesh Kumar Bhuptani1,5 and Abdul Sattar Baloch4
...h 0, 10, 20, 30 and 100% concentrations of honey and after 1, 4, 8 and 12 days of storage (4 ̊C). The moisture, fat and protein contents were moderately decreased on the 4, 8 and 12th days of storage in all the groups. The glycogen content showed a significant (P < 0.05) increase among the groups except for the control. Whereas peroxide and thiobarbituric acid values significantly (P < 0.05) decreased in all the groups, excluding control, which showed a...

Seharish Munawar and Nuzhat Afsar*

...es at Gadani due to high concentration of leached out organometalic compound Tributyltin Tin (TBT) from the ship breaking scrap which is responsible to change hormonal levels and subsequent physiological change related to sexuality in animals as well as also responsible to bring morphological changes in other body parts like antenna and eye spot of affected individual.

...

Mehwish Kanwal1, Muhammad Mushtaq1*, Surrya Khanam2, Irfan Ahmed3, Muhammad Sajid Nadeem1, Amjad Rashid Kayani1 and Tariq Mehmood1

...le egg) at two different concentrations (2.5 and 5% each) were tested for their relative consumption. Groundnut and maize (1:1 ratio) were used as basic bait; for control group, additives were not included in the basic bait. Each experiment was performed under a single choice test pattern. Whole egg at a concentration of 5%, enhanced the intake of the bait significantly over the basic / plain bait, while a non-significant di...

Yue-Xia Ding1, Qun Wu2, Zhao Namula1 and Yi Ma1,*

...easing the antimicrobial concentration. Plasmid conjugation test was carried out by membrane filtration method. The correlation between antimicrobial resistance and virulence traits was analyzed by LD50 and related genes. Sensitive Streptococcus isolates to erythromycin and S. pneumoniae ATCC49619 were induced to resistance in vitro, MIC value was from ≤ 0.5 μg/mL up to ≥ 64 μg/mL, and ermB or mefA resistant gene were carried. Transfer rate of resi...

Anwar ul Haq1, Maqsooda Parveen2, Shehzadi Saima3* and Khalid Masood Ahmad1*

...hese pot plants. Optimum concentration of appropriate auxins is used to promote effective rooting. Completely randomized design (CRD) is adopted and it consisted of five treatments including control and different concentration of both auxins viz. 1-Naphthalene acetic acid (50 mg/L,100 mg/L) and Indole-3-acetic acid (100 mg/L, 200 mg/L) on tip cuttings of chrysanthemum. In this experiment, 15 pots of 9-inch diameter were used...

Muhammad Hasnain1, Qudsia Nazir2, Ifra Saleem2*, Syed Shahid Hussain Shah1, Muhammad Asif Ali1 and Noor ul Ain3

...f EZU showed improved Zn concentration significantly in rice grains, which were maximum (66.8%) and minimum (55.3%) increase was in rice observed as compared to where no (Zn) was applied (in control) at Faisalabad and Chiniot (FSD and CHT), respectively. EZU also showed improved results of Zn contents in grains as compare to the treatment where ZnSO4 and urea was applied separately. On the other hand, with application of EZU, agronomic parameters including str...

Lalu Ahmad Zaenuri*, Rodiah Rodiah, Adji Santoso Dradjat, Oscar Yanuarianto, I Wayan Lanus Sumadiasa , Lukman Hy 

... volume, pH, spermatozoa concentration/ml and concentration/ejaculates, plasma membrane integrity, viability, progressive motility, and morphology, including normal and abnormal spermatozoa. The statistical significance of the results was evaluated by pre- and post-test analyses using T-test with repeated measuring. Data were expressed as Means ± SD and SEM. Results revealed that semen volume, spermatozoa

Imran Ahmad

...s study assesses how low concentrations of microcrystalline invert sugar (MIS) improve the perception of a better mouthfeel when added to tropical fruit and vegetable purees. The study evaluated sensory (organoleptic) attributes corresponding to the textural qualities measured by a texture analyzer. A commercial MIS (size ≤ 40 microns) was added to the puree of five arbitrarily selected fruits (strawberry, banana, and apple) and vegetables (beetroot and car...

Saima Mehar1, Amtul Sami2 and Erum Iqbal3*

...inistered dose of 0.25 % concentration. After 72 hours of treatment, C. cyminum (MeOH) and N. sativa (BeOH) had a non-significant difference in nematotoxic impact (90.0±10.3, 88.0±7.2 mortality %, respectively) in 1% concentration. According to decreasing order, MeOH>BuOH>Chloroform>ether >Hexane, all fractionates showed positive reactivity and potential.

...

Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1 

...ibrio genus. The average concentrations of Vibrio spp. in brood, brood’s tank water, eggs, larvae, larval tank water, and larval feed were log10 3.86±0.61 cfu g-1, log10 3.63±0.57 cfu mL-1, log10 2.73±0.13 cfu g-1, log10 2.86±0.21 cfu g-1, log10 2.93±0.25 cfu mL-1 and log10 3.23±0.37 cfu mL-1 respectively. The highest concentration of Aeromonas spp. (log10 3.62±0.24 cfu ...

Muhammad Zeeshan Siddiqui1, Mujahid Ali2*, Shoaib ur Rehman3, Shahid Iqbal4, Malik Abdur Rehman5, Hafiz M. Tayyab Khan4, Muhammad Ali6, Muhammad Azher Nawaz4 and Saqib Ayyub1

...lar responses toward the concentration of sodium ions according to their genetic potential. Various agronomic traits and physiological traits along with seedlings’ ionic content of sodium, phosphorous, potassium, and sodium in leaves revealed that ‘Round ball’ has better NaCl tolerance ability compared with other genotypes used in this study. The highest ionic sodium concentration (10.34 µg g-1 DW) wa...

Asmaa A. Darwish*, Adel M. El-Kattan, Mona A. Mahmoud, Mohamed T. Ragab, Amani A. Hafez 

... growth hormone, and TSH concentrations and a significant (P˂0.05) decline in the anti-inflammatory cytokine, anti-oxidants, insulin, T3, and T4 levels. The iron profile of infected animals was characterized by a significant (P˂0.05) hypoferremia, hyperferritinemia, and hypotransferrinemia. The estimated pro-inflammatory cytokines and APPs yielded high sensitivity and specificity values in both infected species, but IL-1β, IL-6, and Fb had the highest l...
Nasir Ali Tauqir1*, Asim Faraz2, Abdul Waheed2, Ayman Balla Mustafa3, Irfan Shahzad Sheikh4, Michela Pugliese5 and Shahid Nazir6
... for blood triglycerides concentration, total proteins and blood urea nitrogen according to standard procedures. Nutrient intake, nutrient digestion, nitrogen balance, milk production, milk fat, total solids in milk and solid not fat did not show any treatment effect. However, milk protein percentage was the highest (3.42% and 3.41%) in buffaloes fed diets containing 35 and 25 g/d methionine followed by (3.26% and 3.17%) those fed 15 g of methionine and contro...

Hamdi Mayulu1*, Endang Sawitri2

... results showed that the concentrations of flavonoids, tannins, and total phenolics were 51.325±1.47 mgQE/g, 553.165±34.18 mg tannic acid/g, and 339.875±18.86 mg GAE/g respectively, while the DPPH radical scavenging activity was 58.5 µg/mL at 21 days of incubation (T3). This indicated that black garlic has broad potential to become a natural feed additive suitable for livestock production in line with the development and progress of ...

Munir Ahmad1, Abdul Ghaffar1, Riaz Hussain2* and Rifat Ullah Khan3*

...ted in terms of time and concentration manners. In comparison to control fish, the hematological profile including lymphocyte, hemoglobin erythrocyte and monocyte counts, was considerably (p < 0.05) lower. The final results on the serum biochemical parameters showed significantly (p < .05) higher concentrations of liver biochemical profile, biomarkers of kidneys, triglyceride, glucose, and cholesterol in fish treated w...

Indri Juliyarsi1*, Sri Melia1, Rizki Dwi Setiawan1, Deni Novia1, Lailatul Anshor2, Tomi Candra2 

...ble film. Increasing the concentration of rosella added to the whey edible film also increased antioxidant activity. The use of rosella extract can increase the antioxidant properties (79.60%) of edible films whey and consumer acceptance of color attributes (3.84-like) with the best treatment at an additional concentration of 0.6%. 

...

Yaman Saad Fadhil

...ueous extract in several concentrations (0%, 5%, 10%, 15%) on the chicken meat stored for 12 days at 4°C temperature. The microbiological properties (total plate count, Coliform count, Staphylococcus aureus), and the sensory properties (colour, odour, Brittleness, and general appearance) were investigated. In general, increasing the propolis extract concentration in samples leads to a reduction in the growth rate of the ...

Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1* 

...was highest at genistein concentration 30 µM (P<0.05). The effect of the antioxidant genistein on tris aminomethane - egg yolk diluent gave a significant effect on sperm viability in the Before Freezing (BF) phase at 1.5 hours, BF and PT at two hours (P<0.05). The addition of different genistein in diluent tris aminomethane - egg yolk was no significant effect (P>0.05) on sperm abnormality in both the BF and PT phases at 1 hours, 1.5 hours and 2...

Ayesha Anwar, Sadia Aslam and Nauman Khalid*

... properties. The varying concentrations of beef fat was replaced with 5 to 15% linseed oil-based emulsions. The chemical composition of the patties was affected by the inclusion of the linseed oil emulsion in patties. The cooked and uncooked patties prepared with the 10% and 15% linseed emulsion showed highest moisture and ash content while patties with 20% beef showed highest fat and protein content. There was a significant difference between the pH values of...

Dalia M. Amin1*, Noha Mohammed Halloull1, Walaa E. Omar2, Basma A. Ibrahim2, Nahla M. Ibrahim1 

...ed. In renal tissue, the concentrations of transforming growth factor-1, pERK1/2, and pSTAT3 were assessed. Results: The group that received adenine treatment had considerably higher blood urea nitrogen and serum creatinine levels as well as significant pathological alterations as tubular injury and infltration of infammatory cells. Elevated TGF1, pERK1/2, and pSTAT3 levels were also present. Bcl-2 levels were down while p53 levels were up. All of the detected...

Abdulaziz A. Alaqil*

...nd corticosterone (CORT) concentrations were evaluated in the treatment groups (n=10). Additionally, samples of the spleen and duodenum were taken from layers in each group (n=10) to assess heat shock protein 70 (HSP70) density and glutathione peroxidase (GHS-px) activity, respectively. The general linear model procedure with one-way analysis of variance (GLM-ANOVA) was used to analyze the data. The results showed a significant (P<0.05) increase in the...

Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova* 

...mice using 10%, 20%, 40% concentration of C. leucocladum, no death of mice was recorded within 30 days. When studying the irritating and allergic effects of C. leucocladum on rabbits, it was found that no irritating effect and allergic reaction was observed within 24 hours in animals with 10%, 20%, 40% content of C. leucocladum. Studies of the antimicrobial properties of C. leucocladum extract with 10%, 20%, 40% concentration

Altaf Ur Rahman1, Muhammad Ajmal Khan2*, Ali Hazrat3, Awais Farid4, Muhammad Yahya3* and Inam Ullah5

...fusion method by using a concentration of 100 µg/ml. Furthermore, the antioxidant potential of extract was estimated at various concentration (50, 100, 150, 200, 250 µg/ml) through 2, 2-Diphenyl -1- picryl-hydrazyl (DPPH) assay (150 µg/ml). Results showed that the methanolic extract of C. jacquemontii is a rich source of many phytochemically active compounds such as alkaloids content (3.60%), flavonoids (3....

Zaki Al-Hasawi1* and Reda Hassanine2

...hile their corresponding concentrations were significantly high in the polluted site. Mean concentrations of other metals (Mn, Fe, Ni, Cu, Zn) in water, sediment and fish liver samples from polluted site were significantly much higher than those from the unpolluted one. In contaminated fishes from polluted site, mean levels of blood biochemical parameters, serum liver enzymes (ALT, AST, ALP, GGT), serum glucose, triglyceride...

Zaki Al-Hasawi1* and Reda Hassanine2

...hile their corresponding concentrations were significantly high in the polluted site. Mean concentrations of other metals (Mn, Fe, Ni, Cu, Zn) in water, sediment and fish liver samples from polluted site were significantly much higher than those from the unpolluted one. In contaminated fishes from polluted site, mean levels of blood biochemical parameters, serum liver enzymes (ALT, AST, ALP, GGT), serum glucose, triglyceride...

M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1

...l conditions of elevated concentrations of non-esterified fatty acids (NEFAs). The maturation conditions in the current study were resemble the situation in the oocyte microenvironment of metabolically stressed lactating dairy cows during the post-partum period where the negative energy balance occurred. Oocytes were in vitro matured under elevated levels of NEFAs for 24 h in serum-free maturation media. Obtained zygotes were cultured in synthetic oviduct flui...

Saqib Ali Rustam1, Shoaib Sultan Afridi2, Muhammad Inamullah Malik1*, Shakeeb Ullah1, Syed Muhammad Kamal Shah1, Faiqah Ramzan1, Arsalan Khan3, Israr ud Din3, Wasimullah3, Hafiz Ahmad Raza4

...rsus simple in different concentration on weight gain of Bulkhi breed’s lambs were carried out. The trials were done on 12 lambs of age under 24-30 weeks and also have weight about 30 ±1.5kg. Lambs were grouped as A, B, C and D and each group contained 3 lambs. Groups were fed by four rations as ration containing 4% urea, 2% urea treatment, only sugar beet pulp, and control ration, respectively. After adaptation period of 10 days, the experimental...

Sarfraz Ahmad1, Mian Anjum Murtaza1,* and Kashif Akram1,2

...istant starch) and their concentrations on the meltabilty, stretchability, hardness and yield of low fat mozzarella cheese. On the basis of functional parameters, cheese samples with inulin @ 0.4% and resistant starch @ 1.0% exhibited the best results. Hence, it was concluded that the addition of inulin @ 0.4% and/or resistant starch @ 1.0% as fat replacers revealed the exceptional prospects to produce low fat Mozzarella cheese without much compromising the qu...

Farooq Jan1*, Abdur Rauf1, Ikramullah Khan1, Muhammad Yasin2, Muhammad Qayash3, Hazrat Wali1, Muhammad Luqman1, Fayaz Asad4 and Muhammad Khalid5

...factors affecting pollen concentration in Islamabad. For this purpose, three years data (2009-2011) of pollen concentration and meteorological parameters have been used. Pollen concentration in the atmosphere has been measured using RotoRod sampler. Many plants pollen are allergenic that cause different types of allergenic diseases globally. Pakistan also faces the problem of pollen allerg...

Selvaraj Thanga Malathi¹, Venkatraman Anuradha1*, Mohamed Yacoob Syed Ali2, Muhameed Sajjad Sarwar3, Nagarajan Yogananth2

...us) exposed to sublethal concentration of Lithium [30%] for a chronic period of 60 days. The levels of FT3, FT4 and TSH tested were significantly decreased in treated groups when compared to the normal. Thus, it is evident that determination of thyroid hormone secretion and histological studies (FESEM – field emission scanning electron microscopy) were useful in determining the effect of trace metal pollution and its effect in freshwater fish species.&nb...

Yoshio Hamano1*, Yasuji Kurimoto2 

...ucogenic free amino acid concentrations in antemortem breast muscle. LIP decreased total fatty acid concentrations and monounsaturated fatty acid components. In post-mortem breast muscle, the effects observed in antemortem muscle disappeared. In contrast, AWP increased a component of polyunsaturated fatty acids in the breast meat. Therefore, this study suggested that growth and metabolic responses to LIP and AWP occur almost...

Akbar Hayat1*, Muhammad Asim1*, Tehseen Ashraf2, Ehsan-Ul-Haque1, Rabia Zulifqar2, Maryam Nasir3, Ahmed Raza1, Fahim Khadija1, Sohaib Afzal1 and Shafqat Ali1

...ll various potting media concentrations significantly influenced all the growth parameters of rough lemon citrus rootstock. The growing substances comprising Soil + silt + sand (2:1:1), and Rice husk + sand + soil (2:1:1), shows the prominently significant impact on rough lemon, in contrast to others media. In this experiment, an eight-month-old seedling of uniform height were used. The container was a 14′′x8′′ size plastic bag.

...

Lianci He1, Dingxi Bai2, Chenxi Wu2, Yizhu Zhong2, Shi Chen2, Xinru Bao2, Jing Gao2*, Chaoming Hou2*

...roups received different concentrations of SXC (1.62 g/ml, 0.81 g/ml and 0.41 g/ml), FLU group received 0.21 mg/ml fluoxetine. CFS group and CON group received the same volume of normal saline. All groups received treatment for 28 days. The rats’ body weight, fatigue time, activity and mobility, and mRNA and protein levels of interleukin-1 beta (IL-1β), tumour necrosis factor alpha (TNF-α), transforming growth factor β-activated kinase 1 ...

Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2* 

...rating essential oils at concentrations under 100 µg/ml in semen extenders had a positive impact on sperm quality. Nonetheless, employing essential oils at elevated concentrations of 200 µg/ml exhibited detrimental impacts on the sperm. The optimal dosage for maintaining the integrity of the dog sperm’s membrane and enhancing its property parameters was determined to be a supplementation of 100 µg/ml ...
Maria Azam1, Tahir Mahmood Qureshi1, Saddam Hussain2, Amjad Islam Aqib3*, Shanza Rauf Khan4, Kashif Akram1, Misbah Ijaz5, Maheen Murtaza6, Afshan Muneer6 and Sammina Mahmood7
...owest Minimum inhibitory concentration (MIC) (1.302±0.564 mg/mL) followed by Fe2O3 (2.930±1.691 mg/mL) and ZnO (3.906±0.000 mg/mL). MIC of ZnO was recorded to be 18.23±11.93 mg/mL, 15.63±0.0 mg/mL, 13.02±4.5 mg/mL, 7.81±6.77 mg/mL, 5.21±2.26 mg/mL, and (3.906±0.000 mg/mL) at 04, 08, 12, 16, 20 and 24th h of incubation. A similar trend was followed by other nanoparticles at different hours of incuba...

Jing Hu1,2,3 and Zhenhua Ma1,2,3*

...The changes of serum ion concentration in hybrid grouper over time can be divided into three categories: change near the level of the C group (Na+, K+), roughly higher than the C group (Cl-, Fe3+, P5+) and roughly lower than the C group (Ca2+, Mg2+, Zn2+). The serum urea nitrogen showed an increasing trend, while serum creatinine and uric acid showed a decreasing trend. In conclusion, the simulated typhoon seawater can damage the gills and head kidney tissues ...

Ghulam Sarwar Channa1*, Abdul Razak Mahar1, Inayatullah Rajpar2, Abdul Aziz Mirani3, Saleem Maseeh Bhatti2, Muneer Ali Bhagat1 and Wazir Ali Maitlo

..., 120 and 160 mM of NaCl concentrations. Amongst the five rice cultivars, DR-92 performed better in terms of survival percentage, average plant height, shoot dry weight, percent reduction over control (PROC) for plant height and shoot dry weight, the accumulation of less Na+ and more K+ as well as the higher K+/Na+ ratio in shoot dry matter, than rest of the cultivars at low to high salt-stress (NaCl) conditions. The results show that the non-aromatic rice cul...

Ertika Fitri Lisnanti1,4, Widya Paramita Lokapirnasari2*, Eka Pramyrtha Hestianah3, Mohammad Anam Al Arif2, Zein Ahmad Baihaqi4,5 

...lood profile and glucose concentration of broilers. This study used a completely randomized design (CRD). Treatment is carried out as follows: P0: Control / without adding Marsh fleabane leaf water extract in drinking water, P1: Drinking water plus marsh fleabane leaf extract at a dose of 4ml/litre, P2: Drinking water plus Marsh fleabane leaf extract at a dose of 6ml/litre, P3: drinking water added Marsh fleabane leaf extract with a dose of 8ml/litre, P4: D...
Tehreem Fatima1, Jabbar Khan1,*, Hamid Shafiq2, Dost Muhammad3, Muhammad Rafi1 and Shoaib ur Rehman1
...n control group, maximum concentration observed was 38ng/ml while the minimum serum level was 18ng/ml with median value of 13ng/ml. In longevity males, serum Hsp70 levels increased in individuals between 89 to 91 years of age, peaked between 92 to 97 years but comparatively lower having the age of above 98 years. On the other hand, serum concentration of Hsp70 in longevity females were highest in those having 89 to 92 years,...
Tabark F Ahmed, Nibras Z. Yahya*
 
...gh fractional inhibitory concentration (FIC index < 1) against tested isolate, while the combination of ascorbic acid with erythromycin exhibited antagonistic effects. Furthermore, time-kill analysis showed that ASCORBIC/CIP combination exhibited highest synergistic and bactericidal effect. It was determined that in vitro study against E. coli O157 in the treatment of urinary tract infection, the combination of ascorbic acid and ciprofloxacin was both more ...

Pham Tan Nha*, Le Thu Thuy

... 0.5%, 1%, and 1.5% (DM) concentrations. The trial lasted for 8 weeks, covering the period from 6 to 13 weeks of age for the Tre chickens. The findings demonstrated that supplementing the diet of Tre chickens with Garlic at a concentration of 1.0 - 1.5% (DM) resulted in weight gain and improved feed conversion ratio (FCR). Notably, the G1.5 treatments yielded the most favorable outcomes. In terms of Garlic type, the BG treat...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
.... The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of screened Actinomycetes was in the range of 1.3 - 3.5 mgmL-1 and 1.9 – 4.0 mgmL-1 of bacterial biomass, respectively. Three isolates, S. monticola, S. septentrionalis and S. polaris showed 100 % mortality at 1000 ppm against Anopheles 3rd instar larvae. These findings indicated that actinobacterial isolates possess antibacterial...

Yongman Guo1,2, Jundong Tian1,2,3, Dong Yang1,2 and Jiqi Lu1,2,3*

...ts showed that. (1) mean concentration of fecal cortisol in mating period (AM: 1751.12 ± 199.16 ng/g; AF: 1366.38 ± 117.85 ng/g) were significant higher than that in non-mating period (AM: 705.97 ± 82.86 ng/g; AF: 699.51 ± 56.06 ng/g). (2) there was significant difference in fecal IgA levels between mating and non-mating periods in AF, but not in AM. (3) there were obvious negative correlations between fecal cortisol and IgA levels ...

Ijaz Ahmad1,5*, Haihong Hao1, Pascal Sanders2, Zafar Iqbal3, Saeed Ahmed4, Farhan Anwar Khan5, Zahir Shah5, Muhammad Ibrahim6 and Lingli Huang1

...for low, medium and high concentration samples. The intraday and inter day relative standard deviation were less than 15%. The Cefquinome were found to be stable for more than 3 month at -70oC (degree symbol) and more than 24 h at 4oC. The present method has been applied successfully for the pharmacokinetics study in cattle. After intramuscular administration, the mean peak serum Concentrations (Cmax) was found 2.95μg/mL ...

Mohammed Naji Odhah1,2, Faez Firdaus Abdullah Jesse1*, Bura Thlama Paul3,4, Bashiru Garba5, Zaid Mahmood1, Eric Lim Teik Chung6, Mohd Azmi Mohd Lila7  

...e Immunoglobulin M (IgM) concentration in goats inoculated with C. pseudotuberculosis significantly (p<0.05) increased at week 2 (18.97±0.28 ng/ml), week 3 (33.97±0.59 ng/ml), week 4 (31.94±0.28 ng/ml) and week 5 (12.51±2.42). Whereas, in the mycolic acid group, the IgG antibody titre increased consistently from week 1 to week 3 (20.99±1.96 ng/ml; 15.52±3.78 ng/ml; 14.74±0.34 ng/ml) respectively. On the other...

Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3

...tudy aims to measure the concentration of FGF2 and EGF in follicular fluid, maturation media and Bali cattle embryo culture media. The study used ovaries that came from slaughterhouse and were oocytes mature before fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and em...

Mudawamah Mudawamah1*, Sumartono Sumartono1, G. Ciptadi2, E. Susanto3, Y. Hartoyo4, L. Affandhy5,6

...ted by the total protein concentration. This research implied that it was necessary to provide feed with a minimum crude protein content of 14.4% - 24.22% since the beginning of pregnancy. 
 
Keywords | Fat-tail sheep, Dormer, Suffolk, Crossing, Lambing
...

Arshad Khan, Mohammad Ihsan, Maryam Bibi, Gul Rahim, Fazl Ullah, Komail Khan and Ali Hazrat*

...logical study. Different concentration (0, 50, 100 and 200) mM NaCl were applied at appropriate stages. Salt stress shows reduction in growth of all mays varieties. Plant height in case of control and treated, the mean value was 31.27, standard error 1.16, coefficient of variance was 26.94, range from Minimum 25 cm and maximum 32 cm, leaf length in control and treated, the mean value was 21.50, standard error 1.74, coefficient of variance was 33.45, range from...

Eman M. Abdelhady1, Nader Y. Mostafa1, Mohammed A. El-Magd2* and Ghada A. Kirrella1

...lower minimal inhibition concentrations than the CP. Among these bacteria, E. coli was the most sensitive to CP and CNPs. Minced meat samples treated with CNPs showed significantly lower E. coli and total mesophilic counts than samples treated with CP. The pH values and antioxidant properties (as measured by the DPPH radical scavenging assay) of CNPs-treated samples were significantly higher than CP-treated samples. There were no significant differences in org...
Mohamed A. Abu El-Hamd1*, Abdelslam M. Metwally2, Zahya R.  Ghallab2, Wael A. El-Hamady and Mohamed A. El-karamany1
...nd G4 (40%) in cows. The concentration of progesterone (P4) significantly decreased in pregnant cows compared with non-pregnant in G2 and G3 at artificial insemination (AI), while it was higher at days 7, 14 and 24 post-AI in pregnant than in non-pregnant cows. At oestrus, the P4 concentration was significantly decreased in G1, G2, G3 and G4, in pregnant cow than in non-pregnant of high or low fertility. Economic analysis re...

Abdullah Alsrhani1*, Aisha Farhana1, Shahid Hussain2 and Muhammad Ikram Ullah1

...y was to determine serum concentrations of thyroid hormones, serum thyroid antibodies, and serum 25(OH)-D and underscore the association of vitamin D with thyroid function in HT. A cross-sectional study comprising 150 subjects, including 80 HT cases and 70 healthy controls was carried out. The serum thyroid hormone concentrations (FT4, TSH), serum thyroid antibodies (anti-TPO, anti-Tg), serum 25(OH)-D, serum ferritin, blood ...

Bdour Muhammed Al-Shweily1*, Jawad Bulbul Al-Zaidawi2, Muhammed Jubair Hanawi1 

...eved when using a higher concentration of fungi, specifically 20×10^6 spore/mL, in combination with 100 infective juveniles/mL of nematodes (resulting in an 83.33% mortality rate for larvae and 80% mortality rate for pupae). Remarkably, the mortality rate of the treated larvae surpassed that of the treated pupae at identical concentrations of fungi and nematodes, implying that larvae exhibit greater sensitivity and res...

Omar Berkani1*, Souheila Slimani2, Nora Sakhraoui3 and Cherif Abdennour1

... exposed to CYP. The FT4 concentration decreased significantly and none of the CYP groups was molting. Treatment with PO aqueous extract seems counteracted the toxic effects of CYP, which was confirmed histopathologically by the noticeable amelioration in the testicular tissues. It was concluded that PO aqueous extract co-administration may be promising as a natural protective herb against CYP-induced reproductive toxicity in the male pigeons.

...

Mahmoud Abdelhady Metwally1*, Mona Abdelftah Ali1, Farouk Abdelmohdy1, Mohamed Kassab1, Tarek Kamal Abouzed2 and Khalil Fathy Abou-Easa1

...ere treated with various concentrations of BMMSCs-CM for up to 72 h. The methylthiazolyldiphenyl-tetrazolium (MTT) assay showed decreased proliferation, while flowcytometry showed increased apoptosis and cell cycle arrest in the G0/G1 phase with inhibition of entry into the S phase. RT-PCR showed p53 upregulation and Bcl-2 downregulation in mRNA expression. Moreover, Western blotting and flowcytometry revealed elevated p53 and lowered Bcl-2 protein levels. In ...

Muhammad Nadeem1, Jamshaid Iqbal2, Tariq Mustafa3, Gul Rehman2, Muhammad Faisal Shahzad2, Muhammad Younas4,5*, Aftab Ahmad Khan6, Ameer Hamza2, Abdul Ghaffar1 and Muneer Abbas1

...is, B. bassiana at 7.5 % concentration resulted in the reduction of thrips population per flower (59.42 %) and it was observed more superior than other tested EPFs. Application of B. bassiana resulted highest number of flowers (185.40) with the maximum number of pods/plant (56) followed by M. anisopliae which produced 180.8 flowers and 51.27 pods per plant at the same concentration. Moreover, a B. bassiana caused a maximum (...
Yafang Wang1,2,3, Fugui Jiang2,3, Haijian Cheng2,3, Yifan Liu2,3, Chen Wei2,3, Ce Liu2,3 and Enliang Song1,2,3*
...easing ASIV. The average concentrations of CAT (P = 0.0005), GSH-Px (P < 0.0001), and T-SOD (P = 0.013) increased, and MDA (P = 0.006) decreased with increased feeding amount of ASIV. Furthermore, concentrations of IL-6, IL-8, and TNF-α were not affected by treatments (P >0.05). In conclusion, ASIV improved the growth performance and antioxidant function of calves in a concentration
Rabail Hassan Toor1,3, Raazia Tasadduq2, Jane B. Lian3, Janet L. Stein3, Gary S. Stein3 and Abdul Rauf Shakoori1*
...CQ-H as most efficacious concentration for inhibiting osteoclastogenesis with 57.8% inhibition of TRAP activity. Whereas, in comparison 20ng/ml RSV showed 49.9% inhibition of TRAP activity. Expression of osteoclast marker genes such as NFATc1, ACP-5, CTSK, MMP-9 and CTR corroborated our results. Distinct downregulation of marker genes was observed in CQ-H and RSV treated differentiated cells compared to the positive control. Moreover, the downregulation was en...

H.A. El-Nagar1, A.M. El-Hais2, M.S. Mandouh2, W.M. Wafa1*, A.H. ABD El-Aziz, K.A. Attia4 

...t. Urea-N and creatinine concentrations, and AST and ALT activities decreased (P<0.05) in treated compared with the control calves, with the lowest values for the combination treatment. The treated calves were heavier with higher weight gains than the control one, being the highest with the combination treatment. In conclusion, addition of a combination of 5g bee pollen and 5g black seeds oil/kg in the starter during the sucking period improves live body we...

Salma Jameel Askar 

...ges, and hemoglobin (Hb) concentrations, clotting times, levels of uric acid and liver enzymes were analyzed. RBCs, platelets, PCV%, and Hb concentration all decreased significantly at in the G2 compared to G1 while clotting time, serum uric acid, blood urea nitrogen levels, and liver enzyme functions all increased significantly. Rats in G3 had significantly higher RBCs, platelets, PCV%, and Hb conce...

Wafaa AL. Bnyean1,2 ,Zainab A.H. AL-Mousawi1* 

...nd (11β-HSD) enzyme concentration, while was a significant reduction in serum MDA level in the therapeutic and protective group compared with the positive control group. Our results concluded that glycyrrhizic acid improves the alteration caused by hydrocortisone of hypothalamus pituitary adrenal axis hormones and reduces the free radicals.  

...

Mahmoud Mohamed Ahmed Youssef and Wafaa Mohamed Abd - El-Hameed El-Nagdi

...sh leaves and flowers at concentrations of 2.5, 5.0 and 10.0 % were bioassayed against root-knot nematode, Meloidogyne incognita second stage juveniles (J2s). The obtained results revealed that the tested extracts at 10% caused 100% nematode mortality at 72 h of exposure followed by the other concentrations with the percentages of net mortality ranging from 49 at the lowest concentration o...

Zhipeng Song1,2, Jialiang Xin1,3, Xiaoli Wei1,2, Abula Zulipiya1,3, Kadier Kedireya1,2 and Xinmin Mao1,3*

... (LDL) and blood glucose concentration (BGC) in db/db mice (P < 0.05). The results showed that marein could effectively alleviate diabetic retinopathy, such as oedema, cell body shrinkage, abnormal nuclear morphology, etc., and ultimately enhance retinal function. In vivo and in vitro experiments showed that after a period of intervention, marein regulates diabetic retinopathy by inhibiting the protein expressions of VEGF, PI3K, fibrin (FN) and spleen tyros...

Asmaa A Darwish* 

...tests, and liver enzymes concentrations and a significant (P<0.05) decrease in IL-10, antioxidants, total protein, albumin, glucose, total lipids, cholesterol, minerals, electrolytes, and trace elements concentrations. The DG hemogram clarified a significant (P<0.05) microcytic hypochromic anemia accompanied by neutrophilic leukocytosis and lymphocytopenia, and their iron profile characterized by a significant (P<0...
Mohamed I. El-Katcha1, Mosaad A. Soltan1, Seham M. El-Kassas2*, Mahmoud M. Arafa3, El-Sayed R. Kawarei 3, Karima M. El-Naggar1*
...altered as triglycerides concentration was increased while total cholesterol was reduced in birds received organic and CuO-NPs. So, the organic or Nano forms of Cu could be used as safe alternative sources in layer diets replacing the inorganic Cu without compromising their productive performance.

...
Jaffar Hussain*, Zeenat M. Ali and Syed Farman Ali Shah
...ion when free fatty acid concentration was high. The acidic catalyst preparation was expensive and complicate exhibited slow rate of reaction. The biodiesel used in blend form and diesel engines required the modification. The biodiesel sustainable and release no greenhouse gas. 
...

SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN

...dard procedure. By using concentration 200μg/ml and
400μg/ml methanolic fraction showed 4.57 and 4.86, hexane 1.54 and
5.0, aqueous 1.07 and 2.33, chloroform 0.94 and 3.87, ethyl acetate
18.56 and 28.41, butanol 4.34 and 7.91. Ethyl acetate fraction showed
highest SPF value. Presence of polyphenols and flavonoids provide an
evidence of this plant being traditionally used in the treatment of vari...
FARZANA SIDDIQUE*1, NAZIMA FIRDOSE2, MUHAMMAD ARSHAD2, WARDAH HASSAN2
& SHAFAAT YAR KHAN2
...ues. Maximum
concentration of malathion (0.72±0.02 μg/kg) and deltamethrin (0.75±0.04
μg/kg) was found in cow milk samples collected from Sargodha. Minimum
pesticide residues were found in cow milk collected from Tehsil Bhera.
Both deltamethrin and malathion concentration in milk samples varied in
all zones. None of the samples showed

SHEZA AYAZ KHILJI1* ZAHOOR AHMAD SAJID2 & SONIA GHULAM BARI1

...rassipes L. in different concentrations of industrial effluents of district Shiekhupura. Eichhornia crassipes was grown for 15, 30 and 45 days in different concentrations of the industrial effluents (5, 10, 15, 20 and 100%) prepared with tap water. All physico-chemical parameters in the different concentrations of the industrial effluents were recorded. The values of these parameters incre...

MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR

...inear in
the concentration range of 3 to 7μg/mL. The proposed method was
validated to determine linearity, precision, accuracy, ruggedness and
robustness. The results of above analytical parameters proved that the
developed method could be used for the routine analysis of
levofloxacin in injection dosage form. The proposed method is free
from expensive solvents, chemicals and time consu...

SHEIKH AJAZ RASOOL1*, MUNAZZA DANISH AJAZ2, MUHAMMAD SALMAN RASOOL3, AHSAN SATTAR SHEIKH4 & FIRDAUS KOUSAR BUKHARI5

...en able to downgrade the concentration of bacteria. It has been reported that Lactobacillus reuteri is at par in efficacy in this regard. The aim of this article involves the provision of an update of the present and futuristic approaches and therapeutics to eradicate infections in animals and humans. Miscellaneous approaches include anti-Campylobacter compounds, probiotics, bacterial viruses, and vaccination and bacteriocins. These approaches have shown succe...

Zainab Sattar Ali* , Safa Azhar Razzaq , Layla Hammody Hashem 

...e weeks for assessed the concentration value of FSH, LH, testosterone, and inhibin B hormone for each of groups above. At the termination of experiment were taken in from them specimen of testes for a purpose pathohistology study and assessed the percentage of DNA fragmentation. The current study observed damage in this sperms DNA and alteration of histopathological section. In addition reduction the levels of testosterone, inhibin b, FSH and LH hormones in gr...

Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1 

...ed bacteria depending on concentration. Phytochemicals analysis indicated the presence of alkaloids, phenol, saponins, flavonoids, tannins, and glycosides. The test organism was more sensitive to C. limonum compared to C. aurantium. The MICs ranged from 1.56 to 12.5mg/ml; MBCs, (3.125 - 25mg/ml) and MBC/MIC ratios (2.0-4.0). Furthermore, a time killing curve of each 4x MICs and 8x MICs concentrations of juice extract showed ...

Nusrat Habiullah1*, Shah Nawaz Kumbar1, Fahmida Parveen Samo1, Shamusuddin Bughio2, Asghar Ali Kamboh3, Burirah Rehman Talpur1 

... bilirubin and uric acid concentration as compare to C and D groups. Gross pathology observed in liver of C, D and F groups revealed inflammation, and discoloration, whereas kidneys were found swollen and congested with distended ureters. Major histopathological changes observed in kidneys of group C, D and F like shrinkage of glomeruli with widened bowman’s spaces, necrosis and inflammation. Whereas, liver shows severe dilation of central vein and sinus...

Erni Damayanti1, Herry Sonjaya2*, Sudirman Baco2 and Hasbi Hasbi2

...ility and to measure the concentration of hydrogen peroxide (H2O2) in Bali cattle embryos produced in vitro. 542 oocytes were matured in TCM 199 medium, were fertilized and were cultured using CR1aa medium for 48 h. After 48 h of culture, the embryo developed into 2, 4, 8 and 16 cells. Furthermore, it was grouped based on the stage of development, each of which was re-cultured for 48 h. After that, its development was re-evaluated and H2O2 measurements were ta...

Getulio A. Barcenas Jr.1* and Luisa Marie I. Barcenas2 

...); and ethrel at 100 ppm concentration on the vegetative and reproductive expression of cucumber were studied at the cotyledonary and two-leaf stages. The study was laid out in a split-plot randomized complete block design with 3 replications. Results showed significant differences in plant responses wherein the application of GA3 resulted in the longest vine length at the two-leaf stage (3.24 m) and enhanced the staminate flower production. Ethrel enhanced pi...

Nimra Altaf1, Muhammad Irfan Ullah1*, Muhammad Afzal1,2, Muhammad Arshad1, Samina Khalid3, Naunain Mehmood4

... respectively, at higher concentrations, while Coragen and Proclaim were moderately effective, with mortality rates ranging from 36.7% to 81.7%. The least effective insecticides were Match and Sunitol, with mortality rates ranging from 26.7% to 58.3%. Radiant (-24.57%) and Voliam flexi (-22.19%) were more efficient in terms of feeding deterrence as compared to other insecticides. These results indicate that Radiant and Voliam flexi could be useful in managing ...

Maria Endo Mahata*, Yose Rizal, Zurmiati, Sepri Reski

...aimed to reduce the salt concentration of the brown seaweed P. australis by immersing it in flowing water. We used a completely randomized design (CRD) with different immersion durations (0, 4, 8, 12, 16, 20, and 24 h) of P. australis in flowing water. Each treatment received four replications. We evaluated dry matter (DM), organic matter (OM), crude protein (CP), ash, salt, and alginate contents. The results revealed a significant (p<0.05) impact on OM, CP...

Al-Moataz Bellah Mahfouz Shaarawy1, Wael Mohamed Wafa1*, Ashraf Ali Mehany1, Reda Abdel Samee Ahmed Rezk2, Shereen Kamal Genena1, Mohamed Hamada El-Sawy1 

...R) and pulse (PR) rates, concentrations of cortisol and creatinine, and activity of AST and ALT in serum were significantly increased (P<0.05, 0.01 and 0.001), while TSH, T3, T4, total protein, glucose, cholesterol, Ca, P, Na, and K, ALP activity, and milk yield significantly decreased (P<0.05, 0.01 and 0.001) in summer than in spring in Friesian and crossbred cows. In summer season, Friesian cows showed significant increase (P<0.05, 0.01 and 0.001) i...

Sona Salem El-Nwehy1*, Assem Abbas Mohammed El-Naggar2 and Adel Badr El-Nasharty1

...d as a combined with two concentrations of EM1 (Effective Microorganisms) (1% EM1 and 2% EM1). On the other hand, the control treatment used the entire prescribed NPK soil amendment proportion. Biofertilizer (EM1) applications combined with NPK had a substantial impact on enhancement of the characteristics of vegetative growth which investigated, flower and corm characteristics, Photosynthetic pigments in leaves, and nutrient content compared to the control. P...

Wisit Ketpanyapong1, Kulisara Marupanthorn2*

...upplemented with MOLE at concentrations of 0, 10, 50, and 250 mg/kg body weight (BW). Supplementing Duroc boras with MOLE increased leukocyte, erythrocyte, and hemoglobin levels, and significantly influenced serum antioxidant concentrations. The 50 mg/kg showed the highest serum antioxidant capacity, followed by 10 mg/kg. The boars in the MOLE-supplemented group exhibited a significant (P=0.004) reduction in the percentage o...

I Nyoman Sumerta Miwada1*, I Nyoman Sutarpa Sutama1, Agus Susilo2

...0 ml each with different concentrations, namely 0% (P0); 2% (P1); 4%(P2); 6%(P3) and 8%(P4). The research method used one-way analysis of diversity (at p<0.05) and continued with the Duncan test using the SPSS version 25 system. The results showed that increasing the level of Moringa leaf extract had no effect on pH, water content, and fat but significantly affected protein and ash content (p<0.05). In the color aspect, the L* and a* values ​​signifi...

Naeem Tariq Narejo1*, Muhammad Hanif Chandio2, Faheem Saddar3, Majida Parveen Narejo4, Bushra Ainy Dars5, Hafeez ur Rehman Narejo6, Athar Mustafa Laghari2, Shafiq ur Rehman Shaikh3, Shahnaz Rashid7 and Ghulam Abbas7

...mpact of dietary protein concentration on survival and growth efficiency of monosex tilapia, Oreochromis niloticus which were maintained in aquarium during the period of April-June 2020. Three diets were prepared by using locally available ingredients (wheat and rice bran, powder of mustard oil cakes, whole wheat flour etc.) to have varying levels of crude protein, specifically 35%, 40%, and 45%. The aeration provided to the aquaria was continuous for 24 hours...

Afshan1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Tahira Tayyab1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Syed Weqas Ali7 and Assad Ullah8 

...asal diet with different concentration of selenium powder. Basal diet (BD) was fed to Group A, which kept as negative control group and no dexamethasone (DE) was offered. Group B was considered as (+ve control group) fed with (BD+15mg DE/kg). The group C, was fed with (BD+0.2mg Se + 15mg DE/kg). Similarly, group D was fed with (BD+0.3mg Se+15mg DE). Group E, fed with (BD+0.4 Se+15mg/kg feed). At the end of the trial, two birds were randomly selected for slaugh...

Haneen Imad Al-Sultani1*, Ahmed Obaid Hussain1, Hazar Shakir Saleh2 

...increase in rosuvastatin concentrations 10, 20 and 40 mg/kg, respectively. Conclusion: It was concluded in the current study that the use of rosuvastatin in high doses causes nephrotoxicity.   

...

Afsheen Khattak1, Shahida Naveed1*, Naila Khalid1, Inayat Ullah Khan2 and Tamana Bakht

...encelioides at different concentrations on wheat and maize seeds at the Botany Laboratory Government Girls Degree College KDA Karak. Eight different concentrations of V. encelioides leaves were applied on maize and wheat seeds to check the inhibitory and stimulatory effect, respectively. Whole plant of V. encelioides was collected, dried, powdered, and aqueous extracts were prepared at room temperature. Randomized Complete D...

Ghani Khan1, Saeed Ahmed Soomro1, Abdul Kabir2, Abdullah Iqbal3*,Naik Muhammad Marri 4, Syed Ahmad khan5, Muhammad Roidar Khan6 , Anees ur Rehman1, Muhammad Zakir Khan7 

...solids, ash, and protein concentration were significantly higher in the oxytocin group, while lactose concentration significantly increased in all lactations of the oxytocin group. Higher milk production was also observed in the third lactation of the oxytocin group. This study provides new insights into the effect of oxytocin on the physicochemical parameters of buffalo milk. This indicates that oxytocin treatment can affec...

Frah R Kbyeh1*, Ahmed N. Abedsalih2 

...ium, Potassium, chloride concentration in the blood after three days comparison with negative control and in all experimental period (14 days) which was no significant change (P < 0.05) between positive control (PC) and diclofenac treated groups but combination (ciprofloxacin 3.5 mg/kg+diclofenac1mg/kg) COM1 group and combination (ciprofloxacin 1.75 mg/kg+diclofenac1mg/kg) com2 significant decrease (P< 0.05) after (seven, fourteen) days comparison wit...

Fatemeh Beheshti1, Maliheh Safavi2, Mohammad Reza Akbari Eidgahi1,3, Parviz Kokhaei4, Mehdi Vazirian5 and  Ali Akbar Shabani1,3*

Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7

...cetone and Methanol with concentrations (5,10,15 and 20%) was prepared. Our study showed that maximum zone of inhibition (ZOI) was observed against bacteria for M. Koenigii i.e., 22mm and 23 mm for fungi, while; O. basilicum and S. asoca plants have21 and 22mm MIZD. Methanol 20%has maximum antioxidant activity in O. basilicum whereas, in S. asoca and M. koenigii 20% aqueous showed maximum activity. Total phenolic content (TPC) 80%methanol exhibited high value ...

Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2 

...ity lipoprotein (VLDL)-c concentrations, tumor necrosis factor-alpha (TNF-), and interleukin 10 (IL-10) concentrations. The results revealed the development of signs of incitement such as increased TC and reduced IL-10, decreased HDL-C, and increased TC, TAG, VLDL-C, and LDL-C, along with an increase in inflammatory markers. CPNPs reduced dyslipidemia and inflammation in female rats. We implied the protective impact of CPNPs...

Arslan Muhammad Ali Khan1, Rao Zahid Abbas1*, Zia ud Din Sindhu1, Muhammad Shahid Mahmood2

...s (1.7%). Five different concentrations of A. subulatum essential oil (0.31, 0.62, 1.25, 2.50, and 5% v/v) along with positive (0.1% Cypermethrin) and negative control (absolute alcohol) were prepared to check the acaricidal and repellent effects. A. subulatum essential oil significantly (p<0.05) enhanced the rates of mortality in a dose-dependent manner of adult and larvae of Hyalomma spp. Egg number, egg mass, and larval hatching were also reduced signifi...

Hafsa Saeed, Soumble Zulfiqar, Abeedha Tu-Allah Khan and Abdul Rauf Shakoori*

...ead and cadmium, at high concentrations, are toxic to microorganisms. A chromosomally encoded znt operon plays an important role in multi-metal resistance in some members of Enterobacteriaceae. However, its role has not yet been explored in K. pneumoniae. This study focused on molecular characterization of ZntR, a transcriptional regulator of znt operon. The minimum inhibitory concentration of zinc, lead and cadmium were 10,...
Hina Mumtaz, Muhammad Zeeshan Majeed*, Muhammad Afzal, Muhammad Arshad, Arif Mehmood and Muhammad Qasim
... other chemicals. Higher concentrations of abamectin (6000 ppm), chlorpyrifos (12000 ppm) and chlorantraniliprole (700 ppm) showed 100% larval mortality at 24 h post-exposure. Lambda-cyhalothrin (3500 ppm) showed 95% larval mortality at 24 h and 100% mortality at 48 h of application. The medium dose rate of abamectin (4000 ppm) and chlorantraniliprole (600 ppm) also showed 100% larval mortality at 48 h of application. In semi-field and field conditions, chlora...

Shamsher Ali* and Naheed Baloch

... different 03 and 06ml/L concentrations of each plant seed oil against the lesser grain borer, Rhyzopertha dominica, and Khapra beetle, Trogoderma granarium infesting wheat grains. Observations were made on mortality, weight loss, and insect damaged grain. The results show the maximum mortality 62.0% of R. dominica and 34.3% of T. granarium at a 6ml/L concentrations of C. colocynthis. While lower mortality 1.8% and 5.2% were...

Nusirat Aderinsola Sadiku1, Oluwatoyin Adenike Fabiyi2* and Tesleem Taye  Bello

...BPL was applied at three concentrations of 100, 200 and 400 mg/ml. Results revealed that nematode infected lettuce plants treated with BPL recorded significantly higher (P< 0.05) mean number of leaves, plant height and yield compared to the untreated control. In addition, BPL at 400 mg/ml had substantially higher nematicidal effects on both the soil and root population of nematodes of L. sativa and compared favourably with the carbofuran treatments. We conc...

Nguyen Huu Van1*, Nguyen Thi Mui1, Dinh Van Dung1, Van Ngoc Phong1, Tran Ngoc Long1, Le Tran Hoan1, Le Duc Thao1, Vo Thi Minh Tam1, Ngo Mau Dung1, Bui Van Loi1, Nguyen Xuan Ba1, Ton Nu Minh Thi2, Nishino Naoki2

... values, ammonia and VFA concentrations in rumen fluid between treatments before and 4h after feeding. The pH values remained in critical rumen pH range of 6.0-7.0 for optimum microbial growth and nutrient utilization. Hence, this study demonstrated that increasing concentrate levels in the diets for Phan Rang sheep up to 2.25% or 3.0% of live weight increased DGW, carcass performance and improved economic benefit for farmers without any adverse effects on nut...

Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3

...lar weight of 36 kDa and concentration around 0.973 mg/mL. The BALB/c mice group injected with 100 ng of purified rOmpH exhibited significant protection (80%) when challenged with LD50 of P. multocida B:2. From the obtained results it is evident that rOmpH can be used as subunit vaccine against P. multocida B:2 and the purified rOmpH can also be further used to develop diagnostic test i.e., ELISA to screen and assess the immune response of cattle and buffaloes...

Wei Wan1, Xiao-Li Wang1, Shu-Juan Wang2, Qin Si2 and Li-Qing Duan1*

...lt, with a median lethal concentration (LC50) value of 0.52 g/L. Among the 66 binary mixtures, 19 showed strong synergistic effects, while 21 showed antagonistic effects. The most profound synergistic effect was the mixture of l-carvone and dihydrocarvone, with an expected mortality of 35.2% and actual mortality of 98.4%. Estragole had the highest frequency of antagonistic effect (7 combinations), and the most significant antagonism was observed when combining...

Amal Hammad*, Shaaban Gadallah, Tarik Misk, Ahmed Mourad 

...ogram values and glucose concentrations in XLV and XH, respectively. On conclusion, despite of lower sedation in acupuncture groups than XH, laser acupuncture seemed to be effective in potentiating the sedative effect of low xylazine dose than vibrational acupuncture. Hence, laser acupuncture could be a promising sedative adjuvant in dogs sedated with low xylazine dose due to their poor tolerability to conventional high doses.
 

...

Milena Vlahovic*, Dragana Matic, Marija Mrdakovic, Larisa Ilijin, Anja Grcic, Aleksandra Filipovic, Jelica Lazarevic and Vesna Peric-Mataruga

...c effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium action, while in chronic treatment, the genetic factor (egg mass)...

Rizki Dwi Setiawan1, Zurmiati2, Wizna2, Ridho Kurniawan Rusli2, Ade Trisna3, Surya Aulia4 

...tation at four different concentrations (76 × 106, 69 × 108, 65 × 1010, and 53 × 1012 CFU/mL) was performed using drinking water. Probiotic supplementation resulted in a significant difference (P<0.05) in meat lightness (L*) from 35.21 (control) to 40.91 (treatment group) and water-holding capacity from 64.31% (control) to 72.70% (group treatment), but had no effect (P>0.05) on a*, b*, cooking loss, and meat hardness. The administ...

Roheela Yasmeen1,3* , Faheem Hafeez1 , Umme Ammara1 , Rubab Younas1 , Sibtain Ahmad2 , Zulfiqar Ali3, Zaheer Ahmad Nasir4 

...d (Pb)). In general, the concentration of all essential, trace, and heavy metals was found to be highest in feed followed by litter and air samples. However, Cr, Hg and Pb were higher in litter samples of group A as compared to feed and air. All the samples were statistically analyzed using one way ANOVA. A significant difference of feed was present with litter and air samples within groups (p<0.05) however, no significant differences were recorded among d...

Abdul Aleem Memon1*, Inayatullah Rajpar2, Ghulam Murtaza Jamro2, Javaid Ahmed Shah3

...control, and chlorophyll concentration in leaves declined with increasing% of lime in comparison with the control. Moreover, when compared to the control, SoP application resulted in greater growth parameters, followed by MoP. Further K treatment also increased in K content in shoots and leaf chlorophyll concentration. The shoot K content was increased by 31.14% with SoP and 13.92% with MoP, and chlorophyll

Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2 

...mma-glutamyl transferase concentration(GGT). The results indicated that 200 mg/kg of SiO2 - NPs orally for 4 weeks contributed to a substantial drop in serum TAC-O, an increase in MDA, GGT, PC, and ROS concentration, and attenuation of silica’s oxidative stress status, CP NPs (T3) or SiO2 NPs (T2) administered orally to female rats for four weeks constitutes a case of oxidative stress. The study revealed the protective...
Mohga A. El-Tahlawey l, L.R. Rizkallal , S.A. El-Arnaouty 2 and Amal A.Khalil 3
...to 8% only when used the concentration 2 g / kg seeds. The results in 2008/09 growing season showed that seed dressing with Gaucho was effective in reducing FBNYV from 52.6% in plant without treatment to 5.6% only when used the concentration 2 g / kg seeds. In 2007-2008 growing season from the obvious results found that the better result when we used insecticide Peremore 0.2% with used rouging, the percent of disease in...
Johan Sukweenadhi1*, Stefan Pratama Chandra1, Finna Setiawan2, Christina Avanti2, Kartini Kartini2, Arief Koeswanto3 and Deok-Chun Yang4
...). In addition, the best concentration that can be formulated into the dermal patch is 0.005% (p-value <0.05).
...

Mahdyl , A.M.M.; Hafezl , M.A.; EL-Dougdoug2, Kh. A.; Fawzyl , R.N. and Shahwanl , Eman S.M.•

...o biotic inducers, virus concentration and disease severity (DS). The obtained results from quantification of total SA in induced tomato plants (after 7 days of spraying inducers) showed high level of SA with kombucha &eatment followed by C. inerme and M. jalapa, while mixed (M. jalapa+C. inerme) gave the lowest level of SA compared with healthy and infected controls. On the other hand, after virus inoculation tomato plants showed reduction of virus and di...

Khamiss l , Omaima; El Helaly , Alexandra and Abol Ela 3, Said

...arted fromlO 1-109. Each concentration was prepared to treat five replicates of C35 cell dish. Treated cells were observed daily and counted 5 times within 15 days, and cell viability was detected by using the viability staining (Trypan blue). Mother suspension treatment reduced cell number 30 minutes to 24 hours post application. The concentrations of 104, 10-5 and 10-7 PEG which '.vere selected by the screening test were t...

El Sayed l , Yasmein A.; Ghareeb2 , A.; El-Dougdoug3, K. A. and Bekheetl , H. K.

...d polyhedral bodies with concentration (2.3x107PIB/ml). In our study the morphological structures of S.exigua infected with SeNPV (EG) as well as healthy one were examined by SEM. It was showed complete destruction of external larval body, reduction in each abdominal segment size, pro-legs and thoracic legs reduce in size and appear short and stumpy also the oval shape spiracle converted to circular shape with rupture and disrupted inside tissue  compared...

Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.

...for ELISA detection. The concentrations of IgG and IgG conjugated with alkaline phosphatase were 1.0 mg/ml and 1:1000 respectively. Antiserum produced specific to BBTMV was used for virus detection by using different serological diagnostic methods. The percentages of virus detection in leaves, stems were higher than that in roots; on the other hand the percentages Of virus detection in anthers and pistils were higher than that in sepals.

...

El.-Kadyl, M.A.S; Badr2, A.B.; zein3, Saiwa N. and Khalifa4, M.A.A.

... was 1/1024. The optimum concentrations of IgG and IgG conjugate were 1.0g/ml and 1/1000, respectively.The antigen dilution end point was 1/800. Ribavirin had a higher inhibitory effect (80%) when sprayed at lower concentration (the minimum inhibiting concentration is 0.0001%) before one hr of inoculation. Salicylic acid and Parahydroxy benzoic acid has the same effect (80% reduction) at <...

Ahmed, Amal A. and Fath-Allah, Mervat M.

...values revealed that the concentration of CMV in doubly infected apricot plants is significantly higher than that of single infected plant. Increase of CMV in doubly

...

El-Sayed • l , Eman H; Mahfouze , Sherin A.; Shaltout ,A. D.; El-Dougdoug3 ,Kh. A. and Sayed1 , R. A.

...plemented with different concentrations of 2, 4-D (2, 4 and 6 mg/l) 6-Benzylaminopurine (6, 7 and 8 mg/l), and Sodium Aide ranging from (1, 2 and 3 mg/l) for induction of mutation. In vitro screened plants after rooting were hardened and acclimatized in the glass house and were shifted into the field. Field screening was carried out against two different isolates of (BBTV and BMV) by using syringe method of inoculation. It was observed that, only two banana pl...

Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman

...sing formalin in a final concentration Of 0.1% for 18 hours at 25c0. Evaluation of the prepared vaccine batch based mainly upon vaccination of different groups of specific pathogen free (SPF) chickens using different doses and estimation of the humoral immune response using the hemagglutination inhibition (HI) test. The vaccine provided a faster and more reliable • immune response against the H5N2 viral antigen after immunizing 3 weeks old chicken with a ...

Abdel Razek,B.Omar*and Magda M.Sayed**

...uorescent. Total protein concentration of the prepared LSDV antisera was 0.8g/dl. Separation of anti-LSDV immunoglobulins 1gG were done using ammonium sulphate followed by conjugation with fluorescein isothiocyanate at pH 9.6. The anti-LSDV IgG conjugated fluorescein sterile and was used to detect LSDV in the MDBK cells and gave good results to dilution 1/20 while the reference conjugate to 1/30.

...

M.A. Abo-EInasr l, Kh.A. EL-Dougdougl, M.H. El-Kattan2 and L.A. Salem2

...r plants using different concentrations of seven nutrient chemicals against Zucchini yellow mosaic Potyvirus (ZYMV). These chemicals were. Potassium sulfate, Magnesium sulfate, Ammonium sulfate, Chelated iron, Chelated zinc, Chelated manganese, and salicylic acid. SAR was determined via disease severity. virus concentration and some biochemical changes i.e., the high level of endogenous salicylic acid, proteins related to in...

E.F. Mohamed1 and I.M. Sabra2

...by increasing GA and IAA concentrations. ToMV inhibition percentages induced by GA and IAA varied according to the time of treatment (1, 2, 3, and 4 hours). High percentages of inhibition were recorded for in Vitro treatment. ToMV inhibition of pre-inoculation treatment was higher than that of post-inoculation treatment. IAA was more effective than GA.

...

E.F. Mohamedl and A.A. Owayss2

...wo hours. when used at a concentration of l, 3, 5, 10 and 20% respectively. In vivo experiment, using of propolis immediately pre-inoculation was more effective in reducing the occurrence of symptoms in infected plants than post-inoculation. The same result was obtained after 1 and 2 hours. So. pre-inoculation treatment was more effective in reducing the occurrence of mosaic symptoms on Faba bean plants infected with BBMV than post-inoculation. The best

M.E. Shawky and A.M. Daoud.

...olymerase with different concentrations of other thermostable DNA polymerases with 3' to 5' exonuclease activity (PFU and Vent) was used in the amplification of large fragments (2 Kb, 3 CD genes) of foot and mouth disease virus (FMDV). Such long-distance polymerase chain reaction (LD-PCR) was conducted to reduce mismatch extension rates and improve epidemiological cloning and sequencing Studies. When enzyme mixtures in the reaction were optimized. The best res...

Ausama A. Yousif1,3, Sandy M. Watkins1, Lyle J. Braunl, David J. Hurley2 and Christopher C. L. Chase1

 

...tly reduced the relative concentration of superinfecting but not coinfecting cytopathic and noncytopathic BVDV-2 RNA in BT cells. On the Other hand. superinfection of Singer infected cells with BVDV-2 890 increased BVDV-2 890 RNA relative concentration in MDOK cells. 

...

Luthfiralda Sjahfirdi1*, Lisa Nurfalah1, Deanvi Fahira Wisnuputri1, Entang Iskandar2

...ccurred. The highest E1C concentration was 1181.18 pg/g for K2 and 919.93 pg/g for K5. The lowest E1C was 5.75 pg/g for K2 and 29.95 pg/g for K5. The highest PdG was 19995.81 pg/g for K2 and 18168.68 pg/g for K5. The lowest PdG was 20.85 pg/g for K2 and 504.97 pg/g for K5.
 
Keywords | Captivity, Estrone conjugate, Feces, Nycticebus hilleri, Pregnanediol-3-glucuronide, Reproductive behavior
...

Divanildo Outor-Monteiro1,2,3*, José António Silva2,3,4, José Luís Mourão1,2,3, Victor Pinheiro1,2,3

...d increased butyric acid concentration, compared to their counterparts. When comparing slaughter ages, the 84-day-old rabbits exhibited higher dressing out percentages, lower relative organ weights (liver, kidney, lung and heart), decreased hind part proportions and lower cooking loss values. Furthermore, the hind legs of these rabbits had a greater proportion of muscle and less bone, along with increased luminosity and reduced redness. In conclusion, producti...

Firda Dimawarnita1*, Edwina Gabriela Tesalonika Mustamu2, Yora Faramitha1, Haryo Tejo Prakoso1, Wildan Aulia Noorsy1, Komang Gede Wiryawan2

...tty acids (VFA), ammonia concentration (NH3), dry matter digestibility (DMD), crude fiber digestibility (CFD), organic matter digestibility (OMD), methane gas production, fulvic acid content, and bacterial population. The results indicated that the treatment did not have a significant effect on DMD, OMD, pH, NH3 concentration, methane gas, and bacterial populations. Treatment showed a significant result (P<0.05) on total ...

Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*

...milk-egg yolk diluent at concentrations of 1%, 2%, 3%, and 4%, while the control group received no lycopene substitution. The freshly collected semen underwent macroscopic and microscopic evaluations. Subsequently, the semen was assessed pre-freezing and post-thaw with parameters such as viability, motility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the diluent before freezing exhibited signif...

Estabraq Hayder Khayoon, Ammar Ahmed Abdulwahid*  

...ht, a marked rise in the concentration of malondialdehyde and decrease levels of Total antioxidant in blood serum and behavioral differences like Acrylamide greatly decreased the struggling and ascending time in the swimming test, dramatically lengthens the time needed to turn an animal’s head 180 degrees during a negative geotaxis test and decreased locomotors activity in the Y maze test. Curcumin’s anti-apoptotic, anti-inflammatory, and antiox...

Farid S. Nassar1,2, Osama A. El-Sayed3, Saidi Ouassaf4, Ahmed O. Abbas1,2* 

...s. At the same time, the concentrations of alanine transferase, aspartate transferase, and uric acid were significantly (p < 0.05) lowered. Additionally, the triglycerides were dramatically (p < 0.05) decreased while the high-density lipoprotein composed a higher proportion than the low-density-lipoprotein of the total cholesterols (p < 0.05). Additionally, as dietary FS levels increased, plasma corticosterone (CORT) and malondialdehyde (MDA) levels a...

Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4 

...y albumin with different concentrations is expected to separate spermatozoa. This study aims to identify the quality and movement patterns in Bali bull sexed sperm using freeze-dry albumin. This study was conducted five times for semen collection and using treatments; T1 = Medium concentration of 10% and 30%, T2 = Medium concentration of 15% and 45%, T3 = Medium co...

Ahmed M. Manthoor, Ali H. Saliem* 

...tion, minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), by using 4, 8, 16, 32, 64 and 128 mg/ml concentration of the extract, while 1.562, 3.125, 6.25, 12.5, 25, 50, 100, 200, 400, 800 µg/ml concentration of Ciprofloxacin, on the other hand study mechanism of action of the extract against the same bacteria by ...

Tabinda Nowsheen1, Sayed Wadood Ali Shah2, Ali Hazrat1*, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar4 and Muhammad Ajmal Khan3

...s. In minimum inhibitory concentration assay, the crude methanolic extracts showed significant inhibition against all tested bacterial strains at25, 50 and 100 µg/ml. The methanolic crude extract of A. maritime various parts showed MIC of 37.5µg/ml for S. aureus which is gram-positive bacteria followed by 75µg/ml for P. aeruginosa, (gram negative), and B. subtilis (gram positive) that is nearly similar to the activity of ciprofloxacin (standa...

Taleeha Roheen1, Shagufta Kamal1*, Shazia Anwer Bukhari1, Ghulam Mustafa1 and Saima Rehman2

...deg;C, respectively. The concentration of MG-1 and MT-1 was significantly higher in the soil of Faisalabad region (FSD) as compared to MG-2 and MT-2 in soil of Sargodha region (SGD). Overall FSD soil was observed to be more suitable for methanogenic and methanotrophic growth than SGD soil according to the conditions needed for microbial growth in soil.

...

Aalaa S. Saad1*, Taghreed H. A. Ali2, Nayerah Alatfeehy3, Dalia Elmasry4 

...lled storage. The 30% PN concentration had a size of 19.783 nm and a narrow size distribution (polydispersity index: 0.253) with a distinct chemical composition. PE’s minimum inhibitory concentration (MIC) was 50 and 25 mg/mL, while PN’s was 150, 75, and 37.5 mg/mL. The number of viable L. monocytogenes in the breaded chicken panne with PE and PNs gradually decreased significantly (P < 0.05) on chilling compar...

Wisnu Jaka Dewa1,2, Ekowati Handharyani3*, Sri Purwaningsih4, Silmi Mariya5 

... 125, 62.5 and 31.25 ppm concentration. mesenggerRNA (mRNA) was isolated from WiDr cells using a commercial kit. The mRNA concentration was then measured with a UV-Vis microvolume spectrophotometer at 260/28 nm. Relative expression of bax and bcl-2 to house keeping gene GAPDH was measured on a real time PCR machine. Relative gene expression was calculated using the ΔΔCt method. The result showed that bax gene exp...

Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*

...e effect on testosterone concentration. This research aimed to determine the effect of PGF2α injection and the optimal time for sample collection on improving semen quality and testosterone concentrations in Aceh bull. Aceh bull was used in this study and allotted into two treatments. In treatment 1 (P1), the bull received 25.0 mg PGF2α (Lutalyse™, dinoprost, tromethamine) 1 hour before semen collection, wh...

Nasir Shah1,4, Muhammad Ibrahim1*, Zarnosh Habib2, Kalsoom3 and Zahir Shah4

...olina) and their various concentration (2, 1, and 0.5%) were used for the purpose. Firstly, the artificial diet of fruit flies was subjected to these treatments, while on the other hand chikoo fruits which were used for flies to settle on, were dipped in same concentrations of these plant extracts, and dried under shade and exposed to peach fruit flies for feeding for 15 days. All the three plants exhibited insect repelling ...

Faramin Javandel Soum Sarai1, Mir Daryoush Shakouri2* and Alireza Seidavi3*

...rol, and LDL cholesterol concentrations (P<0.05). All supplements significantly decrease mean corpuscular volume (MCV), percentage of heterophils and the ratio of heterophil to lymphocyte (H/L), and increased mean corpuscular hemoglobin concentration (MCHC) and white blood cells (WBC) (P<0.05). Addition of formic acid lowered the effect of chromium picolinate on performance parameters, abdominal fat and spleen weights ...

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

...actate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in 10 replicated experiments. The results showed that sperm motility, viability, and plasma membrane integrity were significantly higher (P<0.01) when 2-3 mM BHT was added, while acrosome integrity was elevated after the addition of 1 and 3 mM BH...

Rehana Shahida1, Shagufta Naz1*, Tasnim Farasat1, Saima Sharif1, Shah Jahan2 and Farkhanda Manzoor1

...ificantly elevated serum concentration of IL-6 and altered lipid profile in diabetes individual when compared with the control subjects. IL-6 and thrombomodulin was progressively higher in diabetic group. IL-6 correlated positively with thrombomodulin in the control group but an inverse association was seen in the IGT group and revealed that imbalance of the vascular damage initiated and that lead to damage of vascular endothelium in diabetes.

...

Pramudya Andiana*, Moch. Geerhan Miraja Syahdan, Khothibul Umam Al Awwaly, Abdul Manab

... the effect of different concentrations of the combination of bromelain and papain enzymes in hydrolyzing chicken head protein in terms of the physicochemical characteristics and bioactivity of the hydrolysate. A laboratory experimental method using a completely randomized design (CRD) was used in this research. In this study, chicken head protein was hydrolyzed using different combinations of bromelain (b) and papain (p), namely CHP (without hydrolysis); CHP1...

Noor A. Namaa*, Hayder A.N. Al-Zamely

...group, the total protein concentration increased significantly in the Ginseng extracts and Ginseng NPs groups. However, there was no discernible difference in the values of the Ginseng extract or Ginseng NP groups. When compared to the control group, the Ginseng extract and Ginseng NP groups showed increased mitochondrial expression of the SDHA protein. Comparison of the values of the therapeutic groups showed that the Ginseng NP group had higher values than t...

Peixia Yu1*, Lijun Bo2 and Xueyin Song1

...he basic values. The MDA concentrations and CK-MB values of the Fen group went down and the SOD activity went up when was compared with the I/R group. Whereas, cTnI concentrations of Fen1 and Fen2 groups sharply decreased (all P<0.05); the myocardial injury of the Fen group was less the I/R group. Whereas, the MI region and apoptosis indexes of Fen1 and Fen2 groups dropped significantly (all P<0.05); moreover, Bcl-2 pr...

Hilwah Nora1,2, Rajuddin2*, Hafizuddin3, Rachmad Suhanda4, M. Fathurrahman5

...reduction in spermatozoa concentration, showing no statistically significant differences (P>0.05). Similarly, there were no significant variations (P>0.05) observed in all variables related to testis and seminal vesicle morphology. The administration of curcumin did not yield a statistically significant effect (P>0.05) on decreasing spermatozoa quality concerning testicular morphology, morphometry, and seminal vesicle weight. However, a trend was obse...

Moazama Batool1*, Saima Naz2*, Ghulam Abbas3, Ahmad Manan Mustafa Chatha4, Mamoona Mahmood1, Asma Aziz1 and Fatima Yasmin2

...n corpuscular hemoglobin concentration (MCHC) showed a non-significant decrease in treated groups compared to the control group. Serum biochemical parameters, including total proteins, albumin and globulin, decreased significantly (p < 0.05). At the same time, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), glucose and cholesterol were significantly (p < 0.05) increased in the treated groups compared to the c...

Aalaa S. Saad1*, Amira A. Elokle2, Dalia Y. Youssef2, Neven M. Omara2, Samr Kassem3

...Ps showed no toxicity at concentrations ranging from 50 to 450 µg/mL for both nanoparticles. The MIC of CS-NPS and CS-LZ-NPS were 2.5 mg/mL and 0.312 mg/mL, respectively. As well, the number of viable E. coli O157 in fillets with CS-NPs and CS- LZ-NPs gradually decreased significantly (P<0.05) on chilling than the control. Moreover, eaeA and stx2 relative gene expression decreased until the 10th day, reaching 0.3 and 0.41 in the CS-LZ-NPs group and 0....

Zahid I. Mohammed

...lowest serum cholesterol concentration as compared to the control and other groups. kids which were fed diets with Spirulina showed significantly (P<0.05) higher HDL-C concentrations in blood serum. Treatment with 5g of Spirulina (G3) showed significantly (P<0.05) higher levels of HDL compared to the second group and the control group. In conclusion, it has been revealed that Spirulina improves the biochemical paramete...

Shirina Akter Toma1, Shah Ahmed Belal2, Md Abdul Baset3, Md Nazim Uddin3*

...of the spent hen but the concentration of soluble collagen in broiler meat was greater. The spent hen was distinguished from broilers by having more moisture and ash contents (except wing meat of broiler), better pressing loss and Warner-Bratzler shear force value, and less intramuscular fat. Results revealed that broilers had the highest level of potassium except drumstick while spent hens had the highest concentration of m...

Aniqa Qaisar, Shabana Naz* and Sajida Arooj

...ahawalnagar. Heavy metal concentrations and variation in DNA damage were correlated. Only Cr and Mn concentrations were directly associated with the LHead and LComet variations. This study concluded that heavy metals contamination exposure could be detected by a no-invasive way and Wildlife Park Bahawalnagar is most suitable for captivity with the least heavy metal contamination and genotoxicants.

...

Rahib Ali Chachar1, Asghar Ali Kamboh1* and Manatbai Bakhetgul2

... corpuscular haemoglobin concentration) was found higher (p < 0.05) in T3 as compared to T0 groups. White blood cell count was recorded higher (p < 0.05) in T3 as compared to T1 group. Lymphocytes (L) percentage was recorded highest (p < 0.05) in T3 followed by T2, T1 and T0 groups. While, heterophil (H) percentage and H/L ratio were found lowest (p < 0.05) in T3 followed by T2, T1, and T0 groups. It is concluded that moringa and neem leaf meal in ...

Shamsa Jabeen and Javed Iqbal Qazi*

... Their increase in serum concentration have been used as indicators for muscle damage. The aim of the current study was to determine the effect of Lactobacillus rhamnosus administration on CK and LDH levels in the serum of mice following extensor digitorium longus (EDL) muscle grafting. Blood from the intact control as well as experimental and EDL muscle grafted mice was secured on day 3, 5, 7 and 14 post-transplantations. CK activity of control and EDL muscle...

Muhammad Azhar1,2, Rida Ahmad3*, Bushra Nisar Khan3 and Zahra Hussain3

...n corpuscular hemoglobin concentration (MCHC), aspartate transaminase (AST), alkaline phosphatase (ALP), alanine transaminase (ALT), bilirubin, creatinine, blood urea nitrogen (BUN) were determined. Further, different biological, analytical and operational factors which impose significant effects on hematochemical factors were also considered to rationalize the results. Most of the values were in accordance with the mean values published in the literature, but...

Moazama Batool1*, Saima Naz2*, Sheeza Bano1, Sadia Nazir3, Ghulam Abbas4, Ahmad Manan Mustafa Chatha5, Maria Lateef2 and Fatima Yasmin2

...ined by the LC50+ lethal concentration of a tertiary combination comprising lead (Pb), nickel (Ni), and cadmium (Cd) was assessed on two freshwater fish species, Catla catla and Labeo rohita, employing probit analysis. Over a period of 90 days, mortality served as the primary toxicity criterion. The experiments were conducted under consistent conditions of pH (7), temperature (28.00.00°C), and water hardness (198.00 mgL-1), with three replicates for each d...

Zaryab Murad1*, Sobia Bibi1, Shehr e Yar Ahmad1, Mohsin Ali Khan1, Rimsha Sadaf2, Mauz ul Haq1, Umair Manan1 and Muhammad Younas2

...incredibly decreased the concentration of Cd in soil thus reduced their uptake and accumulation in rice plants as AB-DTPA extractable Cd, shoot and grains Cd (Ck: 18, 2.5 and 1.1 mg/kg, 4% BC: 66, 0.9 and 0.5 mg/kg). Therefore, biochar is recommended for better crop growth under toxic metals polluted soil.

...

Wafa’a Mohammad Abduwhab1*, Waseem Ali Hasan2, Mohanad Abdulsataar AL-Bayati3

...mpkin-lidocaine gel at a concentration of 100% and the Nano phytosome pumpkin gel at a concentration of 100%  showed a significant entrapment % and loaded efficiency%,  entrapment %  were 95.2 ± 9.41, and 87.32 ± 8.1 respectively, while loaded efficiency% were 86.84 ± 7.77, and 79.40± 3.72 respectively. As well as, both the Nano phytosome pumpkin-lidocaine gel at a

Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei

...ion in Malon Di-Aldehyde concentration in testes, epididymis tissue treated with tetrodotoxin. The pathological lesion in testes of  animals exposed to toxic dose of tetrodotoxin was characterized by obvious disruption of seminiferous tubules with absence of leydig cells and congestion, in epididymis tissue the most histopathological section showed widening of intertubular distance with evidence of sperm stasis, focal hyperplasia of epididymal lining with...

Sandriana J Nendissa1,2, Meta Mahendradatta3, Zainal3, Februadi Bastian3

...omi fruit tannin extract concentration was effective in enhancing the quality of tuna fish balls. The results of the analysis of variance indicated a significant impact (p >0.05) of Tomi-tomi fruit tannin extract treatments at various concentrations on the water content and protein of tuna fish meatballs However, there is no significant difference on the sensory and total bacteria (p > 0.05) of tuna fish meat balls.&nb...

Shanti Fitriani*, Usman Pato, Yusmarini Yusuf, Emma Riftyan, Evy Rossi

...ucted to obtain a proper concentration of bacteriocin to preserve the fishballs and extend their shelf-life without compromising on quality. In a randomized design, five different concentrations of bacteriocins were used. The treatments in this study were the addition of the bacteriocin concentration, namely B1 (0%), B2 (0.15%), B3 (0.3%), B4 (0.45%), and B5 (0.6%). Samples were stored in ...

ISHRAT FATIMA1, MOAZZAM JAMIL1, AZHAR HUSSAIN1*, MUHAMMAD ZAHID MUMTAZ2, MUHAMMAD LUQMAN1, SAJID HUSSAIN3, SAIF UR REHMAN KASHIF4 & MAQSHOOF AHMAD1

...it dry weight up to 78%, concentration of N up to 20%, P up to 65%, K up to 20%, and protein contents up to 20% as compared to uninoculated control. It is concluded that inoculation of ZSB strains like Bacillus sp. ZM20 and Bacillus aryabhattai ZM31 is an effective approach to improve the productivity of okra (Abelmoschus esculentus L.).

...

SYED MUJTABA HAIDER1, KHIZAR HAYAT BHATTI1*, EJAZ HUSSAIN SIDDIQI1, SYED MAZHAR IRFAN1,`2, & ALLAH BAKHSH GULSHAN2

MAHRUKH SHAHID1, KHAJISTA JABEEN1* & SUMERA IQBAL1

...extract of which various concentrations were experienced in-vitro against tested fungal strain. All the applied concentrations of methanol extract i.e. 1%, 2%, 3%, 4%, 5%, 6%, 7% and 8% effectively inhibited F. oxysporum growth. However, 7% and 8% concentrations were proved to be most efficient in retarding the test fungus growth upto 15%. Partitioning of biochar methanolic extract was don...
ZAFAR IQBAL KHAN1*, KAFEEL AHMAD1, KHALID NAWAZ2, MUHAMMAD NADEEM3, ASMA ASHFAQ1,
BABAR MUNIR1, HAFSA MEMOONA3, MADIHA SANA3, FARZANA SHAHEEN1, NAUNAIN MEHMOOD4, HIRA MUQADAS4, MAHPARA SHEZADI5, IJAZ RASOOL NOORKA6, HUMAYUN BASHIR1,
MUDASRA MUNIR1, ILKER UGULU7 & YUNUS DOGAN7
...of these metals, at high concentrations in soil, can cause severe damage to physiological and biochemical activities of plants. Scarcity of fresh water in agricultural area enforced farmers to use industrial effluent and domestic wastewater for irrigation purpose. Ramzan sugar mill industry located at Chiniot discharges high amount of effluent which is used by farmers for irrigation purpose. Current experiment was conducted in Sargodha, Punjab, Pakistan to ass...

KAFEEL AHMAD1, NIMRA ARSHAD1, ZAFAR IQBAL KHAN1, KINZA WAJID1, HUMAYUN BASHIR1, IJAZ RASOOL NOORKA2, MAHPARA SHEHZADI3, HAFSA MEMONA4, MADIHA SANA4, KHALID NAWAZ5, MUDASRA MUNIR1, IFRA SALEEM MALIK1, TASAWAR ABBAS6, ABRAR HUSSAIN7, MUHAMMAD SHER8, MUHAMMAD FAHAD ULLAH6, MUHAMMAD AKRAM QAZI9, MUHAMMAD NADEEM10, MUHAMMAD RIZWAN QURESHI10

... all samples. The nickel concentration found in the samples of soil, forage and blood was highest in the samples collected from site II (Chak 50 SB). The bio concentration factor found for nickel was highest in the samples of site II. On the other hand, the bio concentration factor for blood and forage was highest in the samples collected form site IV (Shaheenabad Road). The correlation re...

IRFANA IQBAL1*, PAKEEZA TANWEER1, FARKHANDA MANZOOR1, MUHAMMAD NAUMAN AFTAB2, AFSHAN KALEEM3, ROHEENA ABDULLAH3, ASMA ZAFAR4 & MEHWISH IQTEDAR3

...oaked in three different concentrations (50%, 70% & 90%) of each of the three unifloral honey samples for 48 hrs. The filter discs were placed on the agar plates seeded with the individual bacteria. Solitary effect of antibiotic discs (Ciprofloxacin, Imipenem, Ceftriaxone, Amikacin, and Vancomycin) and their synergistic effect were also studied. No bacterial growth showed resistance to honey at any concentration when use...

AAMIR MAHMOOD1, ZAHOOR AHMAD SAJID* & SHEZA AYAZ KHILJI2

...re also primed with same concentrations in petri dishes. After 60 days on final treatment harvesting was carried out, leaf samples were taken for analyzing biochemical attributes (protein contents, antioxidants enzyme activates). A decrease in seed germination percentage from 95.22 (at control) to 25.34% (at 120 mM salt stress) and shoot length from 86.12 (at control) to 42.36 cm (at 120 mM salt stress) was observed. Similar decreasing pattern of growth was ob...

KAFEEL AHMAD1, ROBINA KOKAB1, ZAFAR IQBAL KHAN1, ASMA ASHFAQ1, HUMAYUN BASHIR1, MUDASRA MUNIR1, MUHAMMAD SHER2, KINZA WAJID1, HASFSA MEMONA3, MADIHA SANA3, IJAZ RASOOL NOORKA4, MAHPARA SHEHZADI5, MUHAMMAD NADEEM6, TAHIR MAHMOOD QURESHI6, NAUNAIN MEHMOOD7, ABID IJAZ1, BUSHRA HUMA1, IFRA SALEEM MALIK1, KHALID NAWAZ8 ILKER UGULU9 &YUNUS DOGAN9

... on the evaluation of bioconcentration factor and pollution load index of the contaminated soil. Most of the metals observed in wastewater were present above the permissible limit set by WWF. Cd concentration increased considerably under wastewater irrigation in soil and wheat grains. Zn showed highest bioconcentration. Wastewater irrigated soil exhibited the maximum Pollution Load Index (...

ASIFA BASHIR1, AAMIR MUSHTAQ2* & TOOBA MEHBOOB3

...in (58±1.15 %) at concentration 2.56 mg/ml. Similarly, in vivo studies indicated that Phyllanthus emblica 80 mg/kg significantly reduced the glucose level in diabetic rats up to 166 ± 0.7 mg/dl on day 8th at 8th h as compared to diabetic rats 380 ± 0.7 mg/dl. Similarly, it also reduced the weight in rats up to 274.11 ± 0.97 g as compared to 310.71 ± 0.57 g which was recorded before treatment. It was thus concluded that Phylla...

MUJIBUR RAHMAN1, MUHAMMAD AYAZ KHAN1*, WISAL SHAH1 & ALIA NAZ1

...er, AAS-PEA-700). The Cd concentrations in soil ranged from 2.55 to 4.35 with mean value 3.31 mg/kg, 3 to 8.85 with mean value 5.41 mg/kg, 3.15 to 5.55 with mean value 4.9 mg/kg and 2.45 to 5.25 with mean value 3.93 mg/kg on primary, secondary, tertiary road and control site respectively. While the Pb concentration in soil was 0.85 to 40.85 with mean value 22.83 mg/kg, 13.25 to 35.3 with mean value 22.05 mg/kg, 29.83 to 43.4...

ZEESHAN REHMAN1, RANA ABRAR HUSSAIN1, SHAISTA JABEEN2, SAKHAWAT ALI2, ZAHAR NOREEN1 & IRUM MUKHTAR1*

...ion, Pb2+, Cd2+ and Zn2+ concentrations were analyzed in mostly cultivated/consumed Brassica oleracea. Var. Botyris species in Lahore (LHR), Narowal and Kasur districts of Punjab, Pakistan. The average concentration of elemental Zn in sewage-irrigated samples was the highest (153.4233mg kg−1), followed by Cd2+ (70.47333 mg kg−1) and Pb (65.79667mg kg−1). Results showed higher Pb2+ and Cd2+ level in B. olera...

MUBEEN RIAZ1, KIRAN ZAHID1, SUMAIRA ASLAM CHOHAN1, MUHAMMAD ASHFAQ*2, MUHAMMAD ALI2, RIFFAT SIDDIQUE1, NUDRAT ANEES1 & FARAH KHAN1

...with an increase in NaCl concentration. These results suggested that increase in salt concentration had a damaging effect on genetic stability of O. tenuiflorum L., which in turn affected the morphology and physiology of plant. The present piece of work also highlights the importance of salt stress as biomarker.

...

ABRAR HUSSAIN1, AQSA KHALIL1, SAIRA ZAHEER1, MUHAMMAD LATEEF2 & MUHAMMAD MANSHA1

... insects was assessed at concentration of 2, 4, 6 & 8 mg/ml after 2, 4, 6 & 8 hours of post treatment and dead worms were noted. The 8mg/ml concentration of CEE of Tribulus terrestris L. and Ziziphus mauritiana Lam. exhibited 90% and 93% mortality rate, respectively, after 8 hours of post treatment. The positive control, levamisole showed 100% mortality at 0.5mg/ml concentration. I...

ASADULLAH KHAN1, MUHAMMAD AYAZ KHAN1, ALIA NAZ1, ABDULLAH KHAN1 & NAUREEN AURANGZEB1

...ad (Pb) and Cadmium (Cd) concentrations in lichen along different roads having varied traffic density and to find impact of the metals on its growth in Miandam valley Swat, Pakistan. Dermatocarpon miniatum species samples were collected from control site, primary, secondary and tertiary roads and were investigated for Pb and Cd concentrations and reduction in chlorophyll contents. The data were analyzed using ANOVA, correlat...

ZAFAR IQBAL KHAN1, KAFEEL AHMAD1, HAREEM SAFDAR1, KINZA WAJID1, HUMAYUN BASHIR1, KHALID NAWAZ2, MAHPARA SHEHZADI3, IJAZ RASOOL NOORKA4, MUDASRA MUNIR1, IFRASALEEM MALIK1, ASMA ASHFAQ1, HIRA MUQADDAS5, HAFSA MEMOONA6, MADIHA SANA6, NAUNAIN MEHMOOD7, SAIF UR REHMAN8 & TASAWAR ABBAS8

...wage and tap water). The concentration of cadmium in water, root and forage samples was determined. Moreover, bioconcentration factor, pollution load index, daily intake of metals and health risk index were calculated. In tap water Cd value was 0.54 mg/L and in sewage water, it was 0.56 mg/L. In soil the calculated Cd value was lower than USEPA standards. The maximum Cd in root was calculated in winter forages (1.15 mg/kg). ...
NOREEN RAZA1*, TASNEEM A. SAQIB2, SYED NAEEMUL HASSAN NAQVI3, MUHAMMAD ZAHEER
KHAN2 & MAZHAR HIJAZI3
...erved after high and low concentrations of Lead nitrate were injected to Larus argentatus. Paraffin sections showed major alterations at high and low concentration of Lead nitrate in liver, gizzard and kidney. High concentration of Lead nitrate induced, severe dystrophy along with hepatic necrosis; reduction in thickness of muscular layer of gizzard and marked alterations in loop of Henle ...

HANNANA MARYAM, SANA MAQSOOD & SIKANDER ALI*

...ques. Minimum inhibitory concentration (MIC) and critical dilution assays (CDAs) were performed for exhibiting bacteriocin inhibitory potential against various indicator strains including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Azotobacter sp. It was concluded that Brevibacillus sp. possesses strong potential for laterosporulin biosynthesis. Further manipulation of physical and nutritional parameters improved its biosynthesis in liq...
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
...l assays, the volume and concentrations of extracts used were 0.5 μl (62.5 μg), 1 μl (125 μg), 5 μl (625 μg) and 10 μl (1250 μg). Specific laboratory protocols of antibacterial and antifungal assays were adopted. Absorbance of the broth bacterial culture was measured after 72 hours by using photo spectrometer at the wavelength of 600 nm (A600). For antifungal assays, the volume and concentrations of e...

ZAHRA NASEEM1, MUHAMMAD AMIR IQBAL1, SHAAF AHMAD2 & NABILA ROOHI1*

... and after treatment the concentrations of both enzymes were decreased non-significantly in post therapeutic group. Subclinical group also exhibited non-significant elevation in ALT and AST levels. Creatinine levels were significantly (P ≤ 0.05) increased in both subclinical and overt hypothyroid patients and mildly decreased in follow up group as compared to overt group. Conclusively, positive association of ALT, AST and creatinine with TSH and inverse rel...

ADEEBA SYED1, DILAWAR HUSSAIN2, UZMA RAFI1 & SUMAIRA MAZHAR1*

...ere exposed to different concentrations of CPF ranging from 0, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04 and 0.05 mg/L for 96 hrs in glass aquaria. The 96 hrs LC50 value of CPF for Labeo rohita was found to be 0.01 mg/L. During chronic exposure fish were subjected to 1/3rd, 1/5th, 1/7th and 1/9th of LC50 for 30 days. At the end of the trials, tissues from various organs like intestine, liver and kidney were collected and sections were examined ...

ALIA NASEEM, *SUMERA IQBAL & KHAJISTA JABEEN

...te) solution of 50 mg/Kg concentration was applied to the soil. Cr treated and untreated soil samples were amended with compost and farmyard manure at 2.5 % and 5 %. It was demonstrated that in okra when compared with control, the sugar content and germination parameters were affected adversely by Cr application. The treatments of compost at 5 percent and farmyard manure at 2.5 percent positively affected the yield in both Cr treated and untreated plants. In c...

MUHAMMAD ASLAM1*, MISBAH IRSHAD1, AMINA ASGHAR1, ASAD GULZAR1, ZAHRA NOREEN2, ABRAR HUSSAIN2, SHAHID MASOOD3, MUHAMMAD KAMRAN SHAHID1, RASHAD MEHMOOD4, KAYNAT SALEEM5 & SAJID IQBAL1

...o determine the caffeine concentration, pH and acidity in a few energy drinks brands namely sting, booster six B, gourmet spark and powerful locally available in Lahore, Pakistan, and to compare the results with the labeled caffeine concentration and FDA recommended concentration, which is maximum 400 mg/day. The maximum amount of caffeine determined in Gourmet spark was 9.89 mg /100 ml, w...

Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2

...nt of blood progesterone concentration using ELISA. Seasonal breeding and calving patterns were similar at two locations. The highest proportions of buffaloes were bred during autumn (40.8%), followed by winter (29.5%), summer (19.8%) and spring (10%). While comparing the month-wise patterns, the highest and the lowest proportions of breeding were in November and April, respectively. Likewise, the highest proportions of buffaloes calved in autumn (41.3%), foll...

Laila Ibouzine-Dine1*, Inssaf Berkiks1,2, Mouloud Lamtai1*, Hasnaa Mallouk1, Ayoub Rezqaoui1, Abdeljabbar Nassiri1, Abdelhalem Mesfioui1, Aboubaker El Hessni1

...nt nitrite/nitrate (NOx) concentration was assessed after the reduction of nitrates to nitrites using the Griess reaction. Results revealed three distinct periods during postnatal development. In the first period, between PND1 and PND4, NO expression in both sexes increased, reaching a peak at PND8. The second period (PND8 to PND10) showed a slight decrease in NO expression. In the last period (PND11 to PND17), NO expression showed a high level. In summary, NO...

Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1 

...upplemented with various concentrations of selenium, i.e., group A control (0mM), group B (2mM), group C (4mM) and group D (6mM). Results showed significantly (P<0.05) higher motility (78.1±0.22), morphology (86.5±1.08), membrane integrity (85.6±0.30) and live dead ratio (85.3±0.37) for chilled semen in group B than the other groups. Significantly (P<0.05) improved post-thawed motility (68.1±0.42), morphology (73.5&plus...

Marwa R. El-Deken1, Raouf E. Rizk1, Mona R. Ahmed1, Fouad A. Tawfeek1, Hossam A. Shahba1, Wesam A. Fares1, Ayman M. Khalifah2*

...nting the diets with all concentrations of Glu acid significantly improved feed conversion ratio besides, eggshell calcium and phosphorus compared to those for control. Moreover, both Glu 3 and Glu 4 groups represented best significant improvement of hatchability and hatched chick weight. Highest significant increase of villus height and villus area was observed for chickens of Glu3 group (0.6%) compared with the others groups. While chickens of Glu3 and Glu4 ...

Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei

...ion in Malon Di-Aldehyde concentration in liver tissue treated with tetrodotoxin. The pathological lesion in liver of animals exposed to toxic dose of tetrodotoxin was characterized by dilation and congestion of portal vein, Focal aggregation of mononuclear cells formation granulomatous like lesion, Various form of nuclear necrosis with number apoptotic hepatocyte, nuclear pyknosis with evidence of councilman body in liver tissue, The Immunohistochemistry stud...

Shama Sadaf1*, Ayesha Saeed1, Komal Hassan1, Zeeshan Ahmad2 and Maha Naeem Butt1

...developed by using three concentration levels from turmeric powder. Then apply a dye on silk fabric after binding it with vinegar. The dye’s colour concentration and color fastness after 15 washes. This research is based on quantitative research. This is experimental research. In this study, access the color concentration on different stages and fastness after 15 washes. In a beaker ...

Faheem Akbar1, Naveed Ahmed4*, Maria Mussarat1, Imtiaz Ahmed4, Dost Muhammad1, Toseef Ahmad2, Muhammad Afaq Akbar1, Shehryar Rafique3, Seemab Ali4, Sohail Aslam4, Basharat Hussain Shah4, Fayaz Ahmad4 and Muhammad Abbas Khan4

...on bulbs and phosphorous concentration in onion leaves. When the data were averaged over trichoderma levels, these parameters showed linear increases with sulfur levels up to 50 kg ha-1 and then remained unchanged or showed declining trend with further increase in sulphur levels. The mean onion fresh bulb yield increased from 14.11 t ha-1 o 18.91 t ha-1 with 50kg.ha-1 sulphur. Similarly, application of 3kg.ha-1 trichoderma seemed to be optimum level as further...

Waseem Abbas, Imtiaz Ahmed* and Imran Khan 

...1, 2 and 3%. In CWB, fat concentration at 2 and 3% incorporation remained almost equal to that of CB. The protein content increased “significantly in a dose-dependent manner with the increasing incorporation of these two herbs” when compared with the CB. When the carbohydrate content of both breads was compared, the functional bread had significantly lower values (p, for all trends < 0.05), respectively, as the ratio of incorporation increased. ...

Mehwish Zafar1, Muhammad Shahzad1*, Muhammad Zubair Akram2, Quratulain3, Mehak Shehzad4 and Samreen Nazeer5*

...ltural lands. High level concentration of salts hampers growth of plants and its yield of biomass by impacting major physiological mechanisms i.e., ionic, oxidative and osmotic stress. One possible and climate resilient strategy is to introduce new crops that can bear high level salinity and allow irrigation with saline water. Quinoa has great potential to grow under saline conditions having outstanding nutritious value. Pot based complete block design was con...

Safdar Hassan1, Shaukat Ali Bhatti1, Fawwad Ahmad1, Asad Ullah Hyder1, Muhammad Arslan2, Ashar Mehfooz3, Ijaz Saleem3, Muhammad Umar Yaqoob4,5, Mushrraf Nazir1, Muhammad Sharif1* 

...er dietary Zn (70 mg/kg) concentration than NRC recommended under high-temperature climatic conditions. 

...

Tianhoun Denté Fidèle1,2*, Meda Nãg-Tiéro Roland2, Zabré Geneviève3, Koama Benjamin2, Kaboré Adama1, Tamboura H. Hamidou1, Bélem Adrien Marie Gaston4 

...l assay, five increasing concentrations (0.6; 1.2; 2.4; 4.8 and 9.6 mg/ml) of the two extracts were prepared and tested for egg hatching and larval development in the presence of positive (Albendazole) and negative (PBS) controls. Aqueous and hydroacetonic extracts of C. micranthum significantly inhibited (p < 0.05) parasite life stages comparatively to negative control (PBS). Similarly, concentrations of both plant extr...
Samia S. Alkhalil1, Faisal Al-Sarraj2, Abeer S. Aloufi3, Zuhair M. Mohammedsaleh4, Mamdoh S. Moawadh4, Fayez M. Saleh5, Majid Al-Zahrani6, Waheeb Aggad7, Youssef S. Alghamdi8, Mutaib M. Mashraqi9, Saleh Alshamrani9 and 
Mona H. Soliman10,11*
...the rats and interleukin concentrations, along with histopathology of organs. Results of this study confirmed the immunomodulation and antioxidant capacity of Bacillus licheniformis against 28 µg/kg BW of aflatoxins. 

...
Muhammad Atif Raza1, Muhammad Tariq Javed2, Muhammad Fiaz1*, Muhammad Shakeel3, Muhammad Shahbaz Ul Haq2, Amna Kanwal2, Syeda Maryam Hussain1 and Muhammad Zubair Siddiqi4
...T2, T3 and T4 were given concentration of nanoparticles zinc oxide and copper oxide at different rate; 25+10, 37.5+15 and 50+20 mg/kg/d, respectively. Collected data were analyzed using complete randomized design through ANOVA technique. Mortality percent was found minimum 13.3% in birds under T3 group. Effect of all nanoparticle levels was not different (P>0.05) to that of antibiotic in terms of total serum protein and lipid profile; total glycerides, very...

Iqtidar Hussain1*, Muhammad Inam Ullah Qaisrani1, Abdul Aziz Khakwani1, Zuhair Hasnian2, Umar Khitab Saddozai1, Muhammad Naeem4, Hadia Gul5 and Moneeza Abbass3

...rthenium extracts at low concentrations have also been reported in certain crops. A laboratory experiment was carried out to evaluate the germination and germination indices of maize with estimation of maize growth infested with parthenium allelopathy. This trial was conducted Completely Randomized Design with 3 replications. Parthenium dry powder (5, 10, 15, and 20 %) mixed with soil produced variable responses regarding seed germination and seedling growth. ...

Muhammad Adnan1*, Muhammad Nauman Khan2,3, Barkat Ullah2, Faisal Zaman2, Alevcan Kaplan4, Hubert O. Dossou-Yovo5, Sajid Ali Khan Bangash6, Sana Wahab7, Mehreen Ghazal8 and Muhammad Hassan Sarfraz9

...g/kg soil. The different concentrations of Boron (B) were prepared separately by taking a respective amount of boric acid. Pots without supplemented B constituted the control. No additional supplements were applied to the experimental soil. Each pot was irrigated with 100 mL of distilled water with intermittent intervals of 48 hours. All the treatments were replicated three times considering each pot as one replicate. Through EDX analysis, it was noticed that ...

Fika Yuliza Purba1,4*, Andi Magfira Satya Apada1, Andi Ariyandy2, Irwan Ismail1,4, Subaedy Yusuf3,4

...to the other groups. The concentration of hemoglobin and hematocrit in Group A was significantly higher compared to the other groups. In contrast, the mean corpuscular volume and mean corpuscular hemoglobin in Group A were significantly higher compared to Groups B and C. The serum concentration of albumin in Group A was significantly higher than in other groups, and the serum concentration...

Muhammad Usman1, Majid S. Hashmi1, Ayaz Ahmad1*, Fawad Ahmad2 and Zahid Alam1

...by incorporating various concentrations of stevia powder and storing it within a temperature range of 20±0.4°C. The investigated treatments included: Orange Drink (OD)0 (Juice: 15%, Sugar: 13%, Stevia: 0%, Sodium Benzoate: 0.1%, Water: 72%), OD1 (Juice: 15%, Sugar: 0%, Stevia: 0.065%, Sodium Benzoate: 0.1%, Water: 84.83%), OD2 (Juice: 15%, Sugar: 0%, Stevia: 0.070%, Sodium Benzoate: 0.1%, Water: 84.83%), and OD3 (Juice: 15%, Sugar: 0%, Stevia: 0.075...

Kanwal Razzaq1*, Mudssar Aslam1, Sana Arif2, Ansa Idrees3 and Fatima Mubashir3

...supplementation increase concentration of trace elements retention in silver carp fingerlings. The minimal activity retained by trace elements was observed in diets containing lactic acid. The results presented that best responses showed lactic acid in comparison of other dietary supplemented organic acids.

...

Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*

...nd M9 media with varying concentrations of inducers (IPTG and lactose) and post induction time. Maximum expression of IL-2 was achieved in LB with 0.5 mM IPTG for 6 hours post induction time. A denaturing purification scheme (Immobilized metal affinity chromatography) was employed for the purification of IL-2. The refolding of the purified IL-2 was achieved by stepwise dialysis method using urea gradient (8M-0M). Human IL-2 was recovered from 1 litre culture t...

Marah Salim Hameed1, Ali Ibrahim Ali Al-Ezzy2*

...lood urea and creatinine concentrations. Vitamin E -sodium selenite and vitamin C have important role in enhancement for renal functional activity and clearance of urea and creatinine; They considered potent antioxidant that ameliorates the histopathological effects of sodium nitrate on renal tissue. Concentrations of blood urea and creatinine have no effect on potent scavenging activity of monocytes and macrophages.
...

Noura K. Al-Suwailem1, Nancy N. Kamel2, Ahmed O. Abbas1,3*, Farid S. Nassar1,3 , Dalia A.A. Elsayed4, Gouda F. Gouda1,5, Hosam M. Safaa6

... (RBC), hemoglobin (HBG) concentration, hematocrit (HCT) percentage, albumin level, and endogenous antioxidant activity. Meanwhile, plasma total protein, cholesterol, triglycerides, creatinine, urea levels, and liver enzymes activity were significantly (P<0.05) increased. The carcass yield and meat quality showed significant (P<0.05) deterioration in response to heat exposure. MLM administration to the basal diet significantly enhanced blood metabolite l...

Huma Naz1*, Sajid Abdullah2, Tanveer Ahmed3*, Khalid Abbas2, Syed Qaswar Ali Shah1, Muhammad Rizwan1, Fareeha Latif4, Adnan Ahmad Qazi1, Muhammad Adeel Hassan5

... rohita was kept in LC50 concentration of CPF+END, (1.95±0.02 μgL-1 for 96 h) for 5 days after acclimatization. The blood sample of 5 fish was collected after 24, 48, 72 and 96 h to see the DNA damage and nuclear anomalies. Results of the current study revealed that damaged nuclei (DN%) and genetic damage index (GDI) in RBCs of L. rohita exposed to insecticides mixture was increased throughout the experiment. Similarly, the frequency of micronuclei (...

Saira Jabeen1, Muhammad Avais1*, Jawaria Ali Khan1, Kamran Ashraf2, Aftab Ahmad Anjum3

...etermined across various concentrations in a cohort of 20 locally bred rabbits. For preparation of vaccines, the antigen concentration was adjusted to 10^10 cells/mL, a level determined to trigger highest IHA antibody response in rabbits. Three distinct vaccine types were prepared, each employing different adjuvants. To ensure the quality and safety of these vaccines, quality control assessment was carried out. The efficacy ...

Punjab University Journal of Zoology

December

Vol.38, Iss. 2, Pages 137-236

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