Muhammad Mushtaq

E-mail | mushtaq@uaar.edu.pk

... Further trials with low concentrations of strychnine alkaloid and decreasing the poison-baiting duration are suggested.

 

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Muhammad Rizwan Tariq1*, Muhammad Issa Khan1, Aysha Sameen1, Mahr un Nisa2

 

...urated fatty acid (PUFA) concentration that contributes 8.5% of the total fatty acids. Rabbits were reared for the period of eight weeks. Enrichment of 7% flaxseed in the diet of rabbits significantly enhanced growth performance of rabbits and improved poly unsaturated fatty acids in the meat of rabbits as compared to other groups.

 

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Ghulam Ali Bajwa and Muhammad Arshad Khan

... fertilizer application, concentration of N, P, K and Fe in the soil almost doubled. Zinc and Cu contents decreased significantly (p<0.01). The highest N, P and K were found in mulberry variety PFI-1, M. latifolia and Karyansuban, respectively. The highest Cu and Mn were found in Kanmasi. The highest Zn and Fe were found in Husang and Karyansuban, respectively. A significant variation in uptake and retention of macro- and micro nutrients was found in the mu...

Gultaj Hussain1, Abdur Rahman1*, Tariq Hussain2, Siraj Uddin1, Tariq Ali3

E-mail | abdur_rahman@aup.edu.pk

...f citric and lactic acid concentrations effect were maximum for 5.0%, followed by 3.0% and least for 1.0% on E. coli and Salmonella load on beef surfaces. In addition, citric acid was more effective than lactic acid at all concentrations (1.0, 3.0 and 5.0%) in reducing E. coli and Salmonella growth. The citric acid at 5.0% was found significantly effective treatment in reducing E. coli O157: H7 and S. typhymurium population ...

Mudasir Irfan Dar*, Fareed Ahmad Khan and Farha Rehman

E-mail | irfanmudasir@gmail.com

...ere exposed to different concentrations of the insecticide, ranging from 0 to 40 grams active ingredient per hectare (g.a.i ha-1) through foliar spray. Analyses were done at days 3, 7, and 15 after treatment. Lipid peroxidation rates and contents of proline, ascorbate (ASC), glutathione (GLU), antioxidative enzymes; superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were assessed. Imidacloprid enhanced malondi...

Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3

... ml-1. Median effective concentration (EC50) ranged between 9.87x105 and 21.30x105 conidia ml-1 for 3rd to 5th instars. Median effective time (ET50) ranged between 3.3 days and 8.2 days for different tested concentrations. A significant (p<0.05) 2nd degree correlation between temperature and B. bassiana infectivity was analysed. Beauveria bassiana infection was in range of 27-30ºC. Based on these findings, it is con...

John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1

...ss-react with comparable concentrations of E. coli or L929 murine host cells, the assay did cross-react strongly with members of the spotted fever and scrub typhus groups, making the assay specific only to the Rickettsiaceae family level (Rickettsia and Orientia). When conducted in < 100% human serum or a tick homogenate, the fluorescent aptamer assay maintained sensitive detection of Rickettsia typhi

Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3

...ositive) were having the concentration of aflatoxin B1 higher than the permissible level which is 5 ppb for concentrates as recommended by European Communities. It is concluded that the concentrate available for goat feeding is having aflatoxin B1 and its concentration is higher than the permissible level.  

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Nazish Huma Khan*, Mohammad Nafees and Adila Bashir

... showed that the average concentrations of metals in soils of orchard and non-orchard fields were as chromium (Cr) 56.9 and 52.2 mg kg-1, Ni 29.8 and 27.9 mg kg-1 and Cd 2.46 and 1.11 mg kg-1, respectively. The risk contribution from Cd, Cr and Ni were significant in crop seeds of orchard field (THQ>1). The substantial load of heavy metals in studied fields showed the anthropogenic source of pollution. Therefore regular moni...

 Ishfaq Ahmed, Ihsan Mabood Qazi and Suraiya Jamal

...flour (BRF) at different concentrations (0, 20, 40, 60, 80 and 100 g/100 g) on the proximate compositions and functional properties. It was found that percent protein (8.93–12.25%), ash (0.52–1.01%) and moisture contents (4.47–7.09%) showed significant (P < 0.05) increase, while fat content (1.41-1.17%) showed significant (P < 0.05) decrease by blending WF with BRF. Similarly, water solubility index (WSI), water absorption index (WAI) a...

 Farmanullah Khan, Samad Khan, Wiqar Ahmad and Imran Khan

...ant on Cu, Zn, Fe and Mn concentration. Economic analysis showed that NPK75FYM20 rewarded the maximum net income of Rs. 43108 ha-1, indicating its superiority for profitable wheat yield under rain-fed conditions. It can be concluded from these results that the application of plant nutrients from a variety of natural and manufactured fertilizer sources improve crop yield as well as augment the fertility of soils suffering from water erosion hazards.

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 Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid

 
...ect on human health. The concentration of heavy metals (Pb, Cd, Cr, Fe, Mn and Zn) present in poultry egg and meat were determined in the following three districts; Peshawar, Dir Lower and Malakand of Khyber Pakhtunkhwa by using atomic absorption spectrophotometer. In all the three districts the egg albumen was found to contain significantly higher levels of Pb, Cd and Cr as compared to egg yolk which contains significantly higher levels of essential elements ...

Mian Shamas Murtaza1, Aysha Sameen1, Nuzhat Huma1 and Fatma Hussain2

...dually on increasing the concentration of gums and the samples having guar gum were less hard. On sensory evaluation, the maximum scores were awarded to full fat cheese, while the negative control attained the minimum liking. Addition of gums improved the sensory acceptability of cheese. The sample containing 0.45% guar gum was awarded the scores comparable to the full fat cheese for most of the traits. Hence, it was concluded that hydrocolloids particularly g...

Abida Saleem, Sajida Parveen and Muhammad Jamal Khan

...ssess the equilibrium Zn concentration (EZnC). The difference between the IZnA and EZnC was assumed to be adsorbed. Results showed that FYM, PM or BC yielded lower EZnC and higher Zn adsorption (x/m) at higher IZnA but at lower IZnA< 20 mg Zn L-1 the reverse was observed. The data in non-treated soils were fit to both Langmuir and Freundlich but in treated soils they were fit only when adsorption values for initial IZnA were disregarded. The adsorption, as ...

Sadiq Hussain, Muhammad Sharif, Sarmad Khan, Fazli Wahid, Hina Nihar, Wiqar Ahmad, Imran Khan, Nadeem Haider and Tabassum Yaseen

... P on the basis of its P concentration. The recommended dose of N @ 120 kg ha-1 was applied in the form of vermicompost and urea, while K was applied in the form of sulphate of potash. Spores of mycorrhiza were isolated from fresh growing crop of berseem at the University research farm and used as inoculums in this experiment. Results showed the maximum grain yield of 15.09 and 14.7 g pot-1, highest total dry matter yield of 36.3 g and 36 g pot-1, maximum root...

Muhammad Zahid1, Muhammad Hamid Bashir1*, Bilal Saeed Khan1 and Muhammad Shahid2

... N. barkeri at different concentrations under controlled laboratory conditions. The results showed 25%, 30%, 70%, 50% and 65% mortality occurred by tested pesticides at field relevant doses after 144 h respectively. Missing mites data indicated repellency due to pesticides, was highest at the start of experiment, and then these acclimatized on leaf discs. Thiacloprid having lowest LC50 value 295 and proved toxic for the predator while diafenthiuron and pyripro...

Ayanda Opeyemi Isaac,1,* Oniye Sonnie Joshua2 and Auta Jehu2

 

...exposed to varying acute concentrations of glyphosate and paraquat. The fishes responded, exhibiting different behavioural abnormalities like hyperactivity, abnormal swimming, restlessness, loss of equilibrum and haemorrhage. Observation of opercular ventilation count (OVC), tail fin movemant rate (TMR) and air gulping index (AGI) showed a marked difference between control and exposed fishes, indicating that the herbicides negatively impact on these parameters...

Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*

...typic traits such as low concentrations of FSH, LH and T and delayed puberty. In conclusion, mutations in GNRHR may cause delay in male puberty in Pakistani population.

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Afzaal Ahmad Naseem1*, Maleeha Akram1, Sarwat Jahan2, Kiran Afshan2, Zubaria Iqbal1, Faheem Tahir3, Mazhar Qayyum1 and Syed Shakeel Raza Rizvi1

...=27 boys/age group). The concentrations of GH and T were measured using specific ELISA systems and LGV was calculated for each age/stage group. Pearson correlation r and ANOVA were employed. GH and LGV exhibited age related positive correlations from 1 to 20 years and developmental stage dependent positive correlations during infancy, pre-puberty, early, mid and late puberty/adolescence. T and LGV were positively correlated from 1st to 16th year and negatively...

Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2

...and the maximum nontoxic concentration of the extract was determined using DPPH assay and MTT method, respectively. Serial concentrations of the extract not exceed the maximum nontoxic concentration were used against Vero cells for anti-HSV-1 and ant-HSV-2 effects by plaque assay. The results of the phytochemical analysis showed that the extract has high polyphenolic content while has rela...

Shakeel Ahmed* and Sarwat Jahan

...s. The plasma adipokines concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly increased plasma adiponectin (P<0.001), while decreased leptin (P<0.001) and resistin (P<0.01) concentrations compared to normal fed monkeys. No significant (P>0.05) change in adiponectin levels was observed after NMS injection in both normal and metabolically stressed conditions. NM...

Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4

...ts pH (6.7 to 7.4), high concentration of phosphorus (4.50% and 4.33%) and nitrogen (3.26% and 2.37%) favor seed germination, enhance root growth and soil fertility.

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Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2

...ine oocytes at different concentrations. The results showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos in the ...

Faiqah Ramzan*1 and Muhammad Haris Ramzan2

...ally in different dosage concentrations (1μg, 1 ng, and 10 ρg) to adult male mice twice daily for 12 days. Prostatic citric acid levels were determined photometrically. The levels decreased significantly at all tested doses. Intermittent administration of Kisspeptin –10 negatively regulates the prostatic citric acid levels in adult male mice.

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Muhammad Shafiq and Tahir Maqsood

...arding chemical analysis concentration of boron in both wheat straw and grains increased with B application but there was no effect of fertilizer B on NPK concentration of straw and grains. These results are very heartening; signifying that for any soil, B adsorption isotherm need to be worked out for location precise fertilizer B use.

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Dilawar Hussain* and Abdul Mateen

...at both 2 and 4 ppm AFB1 concentrations have almost the same effect on the growth parameters tested except in the case of PER. T3 (0.5% 4TX+2 ppm AFB1) showed better PER (31.42±1.74) when compared to T4 (0.5% 4TX+ 4 ppm AFB1) group (27.76±0.67). Interaction between different growth parameters of tilapia fed AFB1 and 4TX supplemented diets showed that net weight gain (NWG) was significantly (p<0.01) correlated with average daily gain (ADG), spe...

G.E. Odo1*, E.J. Agwu1, N.I. Ossai2, C.O. Ezea3, J. Madu1 and V. Eneje2

... and MCHC to all exposed concentration of aluminium phosphide, significant increase in neutrophil, basophil, eosinophil and monocytes throughout the duration of exposure and decrease in the lymphocytes . The results on gill and liver tissues sampled showed concentration and time dependent significant increase (p < 0.005) in the values of malondialdehyde, catalase, superoxide dismutase, reduced glutathione, alkaline phosph...

Nighat Sultana1,2,*, Shazia Iftikhar1, Nafeesa Qudsia Hanif2 and Iffat Tahira2

... fodder, with an average concentration of 9.49 and 8.36ng/g respectively. Aflatoxin B2 was detected in only two samples (16%) (1.2 and 1.3ng/g), aflatoxin G1 and G2 were not found. Ochratoxin A was found more frequently in fresh (54.16%) than in ensiled (20.86 %) samples with a mean of 8.06 and 4ng/g respectively. Average values for total aflatoxins (TAFs) and OTA were below the permissible limits as per regulation of European commission (EU), i.e. 20ng/g and...

Wang Tian1, Huayong Zhang1,*, Jian Zhang2, Lei Zhao1, Mingsheng Miao3 and Hai Huang1

...ich provides the maximum concentrations of nutrients. Zooplankton communities were more correlated to phytoplankton community biomass than environmental factors. Results of canonical correspondence analysis (CCA) revealed that water temperature, Secchi disk depth (SD), total phosphorus and phytoplankton biomass were the most significant factors that influenced zooplankton. Strong correlations between SD and Brachionus leydigi, Chrysophyta and Diaphanosoma leuc...

Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

...indicates that Pb and Cr concentration differ significantly between AA, AG and GG genotypes of SOD1 (rs2070424) gene polymorphism in RA patients as compared to control group (p<0.05). The present finding indicates that SOD1 (rs2070424) gene polymorphism is not associated with pathogenesis of RA; however levels of Pb and Cr may impart strong influence towards the development of RA in the presence of this polymorphism.

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Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1

...rols. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts was also determined. Syzygium aromaticum was proven to have excellent antimicrobial activity against all tested microorganisms, while C. sinensis and M. piperita showed weak antimicrobial activity. Methanolic plant extracts exhibited greater antimicrobial activity than aqueous extracts. Maximum zone of inhibition exhib...

Sidra-Tul-Muntaha1, Muhammad Sagheer1*, Mansoor-ul-Hasan1 and Shahbaz Talib Sahi2

... Punjab, Pakistan. Three concentrations of each plant extract (5, 10, 15 and 20%) and synthetic pyrethroids (0.01, 0.02 and 0.03%) were evaluated in this study. We observed significant results with each treatment. For both repellent and growth inhibitory effects, A. indica and deltamethrin were most efficient among plant extracts and pyrethroids respectively. At highest dose rates, more than 90% repellency was recorded with both A. indica and deltamethrin. Upt...

Salma Shaheen, Mumtaz Khan, Muhammad Jamil Khan, Saleem Jilani, Zarina Bibi, Muhammad Munir and Mehwish Kiran

 

...M. Maximum Fe, Cu and Mn concentrations were found where pressmud + EM was applied. Dietary fiber, vitamin C and crude proteins were also increased in spinach with pressmud + EM application. It was concluded that EM-inoculated pressmud has higher potential to increases soil fertility as well as stimulate spinach growth and quality. Therefore, warrants further testing under field conditions.

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Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

...ethod. However, the same concentration of crude (1 µg.mL-1) showed 62.20 ± 2.51% growth inhibitions against Fusarium oxysporum using agar well diffusion method. Further investigation, including various chromatographic and spectroscopic analyses on the separation and isolation of potent metabolites is suggested to get a useful lead structure for development of novel antibiotics to combat resistant pathogens.

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Önder Duysak*andKübra Azdural
...s of Iskenderun Bay. The concentration of heavy metal in P. caerulea tissues tended to vary significantly between season and stations.The distribution of heavy metals in P. caerulea followed in their muscular tissues in order; Çevlik > Kaleköy> Payas > Arsuz > Dörtyol > Konacık > Yumurtalık > İskenderun for spring and Kaleköy > Payas > İskenderun > Arsuz > Dörtyol >...
Serkan Sayiner1* and Hilal Karagul2
... evaluated whether these concentrations were adequate. Heifer blood, feed samples including concentrate feed or roughage were analysed. It was found that there are significant seasonal differences for each region and regional differences in each season were due to different feeding conditions. Heifer blood selenium and plasma vitamin E levels were generally found within adequate ranges in all regions for both seasons. We suggest that feed supplements containin...
Sumra Ashraf1, Zain ul Abdin1,*, Saqi Kosar Abbas2,Rao Sohail Ahmad Khan3, Muhammad Tahir1, Sehrish Rasool1, Maryam Anwar1 and Fiaz Hussain1
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...n corpuscular hemoglobin concentration (MCHC) and platelets while total leucocytes count (TLC), neutrophils, eosinophils, basophils, lymphocytes, monocytes, red cell distribution width (RDW) and mean corpuscular volume (MCV) were increased significantly (P < 0.05) which indicated hemolytic anemia. While, serum biochemistry showed significant elevation in alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nit...
Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
...ceps femoris muscle, the concentration of N-isopropyl oxamate in blood was 0.08 mmol/L; 3) in skeletal muscles of the inhibitor group and the control group, the LDH activities were 6.30±0.50 U/mg and 10.01±0.59 U/mg, the contents of LD were 0.28±0.039 mmol/g and 0.84±0.16 mmol/g, and the ATP level were 8.15±1.03 nmol/mg and 12.06±1.23 nmol/mg (P<0.01); 5) the inhibition rates of N-isopropyl oxam...

 Abdul Wajid Khalil and Zafar Iqbal

...00g) was found in higher concentration in aerial parts than roots while some essential amino acid, Tryptophan (6.23±0.06g/100g), Arginine (3.49±0.08 g/100g), Phenylalanine (1.77±0.08 g/100g) were recorded higher in roots as compared to the aerial parts of B. procumbens. It is concluded that aerial parts may serve as a supplement for human diet as well as fodder for livestock. Detailed studies on amino acid of roots are recommended to deter...
Salman Khalid1,*, Abdul Rehman2, Mohammad Yasin Amir1, Abdul Rehman1, Imran Sarfaraz1 and Muhammad Asif1
...he values of PCV (%), Hb concentration (gm/dl) and RBCs during tick infestation.
...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

... chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in egg...
Xiang Nong1,2,3,*, Yao-jun Yang1,2, Guang-you Yang3, Feng-zheng Chen2, Mei Tang1,2 and Gang Wang2
...rol was toxic under 0.5% concentration. Treatment under this condition also showed mite mortality of 16.67% within 12 h and 23.33% within 24 h. The insecticidal activity of stigmasterol was weaker than that of ageraphorone compound obtained from E. adenophorum Spreng. Through preliminary separation. However, in the experiments, we found an interesting phenomenon, that is, the dead state of mites treated with stigmasterol was similar to that of mi...
Adel Eid Mohamed Mahmoud*
...ues and ammonia nitrogen concentrations did not show any significant differences among groups.No significant differences were observed in meat chemical analysis of slaughtered animals among different groups. Animals fed BBP recorded insignificant increase in growth rate by 9 g daily with improvement in feeding cost by 10%.Bakery waste can replace corn grains and wheat bran in concentrate feed mixtures up to 60 % in growing lambs rations without any adverse eff...
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...of the extracts at known concentration of chlorogenic acid were then applied against different bacterial strains for antibacterial activity. The minimum inhibitory concentration of isolated compound was also measured. Two antibiotics were used as references compounds for antibacterial activities. The aqueous ethanolic extract having 968.125 µg/ml of chlorogenic acid inhibited significantly the growth of tested bacteria...

A. K.Bhattacherjee and B. K. Pandey

Dissipation of carbendazim in mango after pre- and post-harvest treatments
...at 0.05 and 0.1 per cent concentrations, respectively, following first order rate kinetics. The corresponding values in fruit pulp after 12 days were 0.57 and 1.26 mg kg . In another experiment, cold water dip treatment in carbendazim solution @0.05 and 0.1 percent was given for 10 min to a separate lot of fruit having no pre harvest spray. Dissipation of carbendazim in whole fruit again followed first order rate kinetics with 0.92 and 2.06 mg kg of the fungic...

P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi

Botanicals for control of anthracnose of bell peppers
...rom C. aromaticus at 50% concentration showed 42% radial mycelial growth inhibition in poisoned food technique, whereas 15% increased seed germination and 26% reduced disease incidence is recoded when compared to untreated control. In pot experiments, there is an increase in height and weight of the plants by 22% and 44% respectively in seeds treated with C. aromaticus when compared to chemical fungicide Bavistin and also 13% reduction in disease incidence is ...

M.S. Islam, M.Ali, and I. Ahmad

Efficacy of sedomil 72WP and recozeb 80WP to control die-back (Colletotrichum gloeosporioides) of tea in Bangladesh
... 1200, 1600 and 2000 ppm concentrations. Sedomil 72WP and recozeb 80WP caused colony growth inhibition, respectively by 75.00-90.77% and 75.88-91.33% in 2008 and 74.66-90.55% and 75.66-91.11% in 2009. In a field experiment at the main farm of Bangladesh Tea Research Institute (BTRI), both fungicides were applied as foliar spray for three times at 1.0, 1.5, 2.0 and 2.5 kg ha-1. In 2008, sedomil 72WP gave 45.52-92.78% and recozeb 80WP gave 46.47- 90.39% reductio...

A. Sajeena and T. Marimuthu

Efficacy, stability and persistence of Ganosol, a Ganoderma based fungicide against plant pathogens
...as the best emulsifiable concentration for use against the pathogen. The pre inoculation spray of the formulation was observed to produce 63.62% reduction in lesion height caused by the sheath blight pathogen. Two isoforms of poly phenol oxidase, PPO 1 and PPO 3 were observed to be induced only in Ganosol 10EC sprayed plants and were absent in control. The formulation was tested to have good emulsion stability. The antifungal activity of the formulation persis...

D.K. Hazra, Megha Pant, S.K. Raza and P. K. Patanjali

 

Formulation technology: key parameters for food safety with respect to agrochemicals use in crop protection
...hains where they undergo concentration and pro-duce potential, long term, adverse health effects. Pesticides in developing countries in Asia and Pacific region are mainly available as dust, wettable powder, emulsifiable concentrates, solutions, etc. These types of formulations are regarded now as ?conventional‘, ?old technology‘ or ?classical‘ or ?traditional‘ because of their increased in dose rate or repeated applications to get desir...

Anil Sehajpal, Saroj Arora and Parminder Kaur 

 

Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...bial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungitoxicity even at the lowest concentration, i.e. 100 ppm, with relative magnitude ...

Shrinivas Mudigoudra and Shekharappa

Evaluation of plant products aganist sorghum shoot fly, Atherigona soccata Rondani
...onosperma, Aloevera @ 5% concentration and were compared with carbofuran 3 G @ 30 kg. ha-1 to know its efficacy on shoot fly in sorghum ecosystem. The results clearly indicated that NSKE (5%) recorded less eggs of shoot fly (0.40egg/plant), per cent dead heart (14.97%) and maximum grain yield of 13.22 q.ha-1 among different plant products. The next best plant product was found to be V. negundo @ 5% in recording less incidence of shoot fly and obtaining higher ...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...rrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin. Camel raw milk produced 2.4 mg/mL of lactoferrin. The lactoferrin isolated from camel milk exhibited promising antibacterial activity against E. coli and Staphylococcus aureus. Concentration of lacto...
Sohail Ahmed, Muhammad Arshad and Babar Hassan*
...ee resins types in three concentrations were applied by brushing and dipping on wooden stakes which were exposed to termites’ for a period of 4 weeks in the field. Weight loss before and after resin treatment was indicative of effectiveness. Highest concentration of all resins significantly reduced the chances of termites’ infestation as compared to other concentrations by dipp...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...lids not fat and lactose concentrations differed (P<0.001) among the treatments but did not show (P>0.05) any linear or quadratic trends with increasing level of enzyme in the diet. Milk protein concentration linearly (P<0.001) increased with increasing level of enzyme in the diets. It is concluded that fibrolytic enzyme addition in the diets of lactating Nili Ravi buffaloes improves the dry matter intake and diet d...
Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...llel to increase in dose concentration, as compared to control and vehicle control. Morphometric studies showed significant differences in nervous, integumentary and skeletal systems against control. Exencephaly, Omphalocoel, hygroma and limb abnormalities were recorded. It is shown that Cr VI causes teratogenic effects in mice embryos.
...
Obaidullah1, Zafar Ali Shah1* and Sami Ullah2
...;g/ml and 80 µg/ml concentrations versus their counterparts of Lapachol and above mentioned metallic salts.

...
Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
...ed that RBC, MCV and MCH concentrations were significantly higher (P < 0.05) and the serum total protein and Mg concentrations were significantly lower (P < 0.05) in male compared to female peafowl. In conclusion, the present study provides base-line values for hematology and serum biochemistry of apparently healthy Indian adult blue peafowl of Thar and can be used as reference values to evaluate the health or diseased...
Muzammil Sattar
...ed in laboratory at four concentrations, i.e., 1, 3, 5 and 7 mg/ml in diet. Significantly higher fecundity 785.12±25.7, fertility 89.23±0.36 % of eggs and longer oviposition period 42.13±1.11 days were recorded in adults feeding on a diet containing Nu lure followed by protein hydrolysate (control diet). The F1 progeny larvae that developed from adults consuming (Nu lure 5ml) diet had the shortest larval and pupal periods...
Mehwish Faheem1,*, Saba Khaliq2 and Khalid Parvez Lone2
...i> was exposed to graded concentrations of bisphenol-A (10,100,1000μg/l) for 14 days. BPA exposure strongly elevates vtg mRNA level in fish exposed to 100μg/l but at 1000 μg/l exposure of BPA, vtg level decreased compared to 100µg/l exposed fish. These results showed that BPA has estrogenic action and cause endocrine disruption in juvenile C. catla at environmentally relevant concentration...
Ayesha Ilyas1, Hafiz Azhar Ali Khan2,* and Abdul Qadir1,*
...ta (Saunders), at 2% concentration in a free choice bioassays. Acetone, chloroform, petroleum ether and ethanol were used for extraction from leaves. Amongst the various treatments applied, the acetone extract of D. alba showed the highest repellency of 84.14%, whereas, the lowest repellency of 10.73% was observed by the chloroform extract of T. peruviana. Significant ovipositional deterrent effects of the extracts applied have been observed....
Hazrat Ali1,*, Wajid Ali1, Karim Ullah1, Fazle Akbar1, Sher Ahrar1, Irfan Ullah1, Irum Ahmad1, Ashfaq Ahmad1, Ikram Ilahi2 and Muhammad Anwar Sajad3 
...rophotometer. Highest Cu concentration of 4.52 ± 0.24 mg kg−1 wet weight was observed in muscles of M. armatus at Chakdara on River Swat while highest Zn concentration of 18.00 ± 1.88 mg kg−1 wet weight was observed in muscles of S. plagiostomus at Daggar on River Barandu. A site wise comparison of both Cu and Zn concentrations ...
Noor Islam1, Muhammad Amjad2, Ehsan ul Haq3, Elizabeth Stephen1 and Falak Naz4
... acid at three different concentrations of 25%, 50% and 100% and control were used against T. clareae in naturally infested honey bee, A. mellifera colonies in Honeybee Research Institute, National Agricultural Research Centre, Islamabad, Pakistan. The percentage of infestation by T. clareae on worker brood, number of dead/fallen mites per hive/weekon white formica sheet, percent mite mortality and honey yield per colony was determined bef...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...ckens with two different concentrations of Egy-H5 plasmid DNA 15µg and 60 µg. Results demonstrate that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vaccine. The Egy-H5 plasmid DNA...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3

 

...ved more fatal at higher concentrations than the individual application at lower concentration for pupation and adult emergence of S. litura. Apart from untreated larvae (control) maximum pupation (66.01±1.32 and 73.95±1.28 %) and adult emergence (61.87±1.71 and 67.77±1.11 %) was observed when larvae were treated with NPV (1x107 POB/ml) against 2nd and 4th i...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...ogens at three different concentration viz., R, R/2 and R/4. Combination of bio agents (T+P) gave maximum results at R concentration against R. bataticola and M. incognita.
...
Kulsoom Akhter1, Tahseen Ghous1,2,Saiqa Andleeb3,*, Faiz-ul-Hassan Nasim4, Samina Ejaz5, Zain-ul-Abdin1, Bilal Ahmed Khan1 and Muhammed Naeem Ahmed1
...7401. At maximum studied concentration of metal ions (250 mgl-1), in above sequence, the strains accumulated 118.2, 121.87 and 90 mg/g Cr (VI), 135, 127.5 and 146.25 mg/g Ni (II) and 120, 129.37, 135 mg/g Cd(II). Langmuir and Freundlich models were applied and found suitable to describe biosorption of selected metals by the isolated strains.
...

Fawad Ali1*, Hidayat Ullah2, Ikhtiar Khan1

...ertain toxic heavy metal concentration in the wastewater, irrigated soil, their accumulation in vegetables, and potential human health risks. The results reveal that Cr concentration in the wastewater, Cd and Zn in soil and in vegetables have been found well above the permissible limits of World Health Organization (WHO) which may lead to a detrimental effect on soil quality, leading to food contamination. The extractable
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...>oC, Protocol III (concentration of dye in staining solution); slides were stained with 1, 5, 200, 500, or 1000µg/mL solution. The slides were observed, intensity of staining and debris presence in samples. The debris presence was reduced (P<0.05) due to washing of semen sample. Intensity of staining increased (P<0.05) due to washing and heating during incubation in tampon solution. However, single and double washing and type of buffer did no...

Syed Tanveer Shah* and Muhammad Sajid 

...lphate) at three calcium concentrations (0.5, 0.75 and 1.0%) stored for 30 days at 10 days interval. The experimental results showed that calcium sources, concentration and storage duration significantly affected all the studied attributes. More fruit firmness (5.57 and 5.69 kg cm2), less Total soluble solids [TSS] (8.57 and 8.26 °Brix) and TSS-acid ratio (12.62 and 11.48) were recorded by the alone application of calciu...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*

 

...ere exposed to different concentrations of LET. At 130 dph, sex ratio was recorded. The results showed that continuous exposure to LET induced the changes of sex ratio. High concentration of LET can promote male-biased sex ratio. In order to reveal the molecular mechanism by which LET caused sex reversal, juvenile carp (120dph) were exposed to 0, 5, 25, 125, and 625μg/L LET for 15 days, and the expression profiles of seve...
Asad Abdullah1, Muhammad Irfan Ullah1,*, Muhammad Waqar Hassan2,    Samina Khalid3, Yasir Iftikhar4, Muhammad Arshad1 and Jaime Molina-Ochoa5,6
...ed at 6% NSE while at 2% concentration of NSE after 148 hrs, 39. 16% reduction was observed. While maximum reduction on non-Bt cotton at 6% recorded as 66.60% and minimum at 2% concentration recorded as 48.72% of neem seed extract against B. tabaci. In case of A. biguttula, maximum reduction in population was observed at 6% concentration of NSE (64.94%) and minimum at ...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...l acetate. The sublethal concentration of ethanol based extract of C. colocynthis increased larval and pupal duration as compared with control. The percent pupation, adult emergence and pupal weight decreased in treated populations as compared with control populations. The results of this study can be used in integrated pest management program for the management of H. armigera.
...
Nargis Bano and Khalid Mahmood Qureshi*
...lt suggests that SA at a concentration of 3mML-1 concentration could be used commercially to improve yield of strawberry.
...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1,  A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...xtender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm characteristics such as sper...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...tively. MgCl2 concentration did not affect and added at 2.0 mM. Four different culture methods were compared for their efficiency for avian mycoplasmas culture. The B method (parts of trachea) were found most efficient for producing growth within 36 h while method A produced within 48 h nevertheless both had the same positivity (70%). While other two methods C (filtrate into broth) and D (filtrate onto agar) produced just 40% and 31%. Moreover, the ...
Jiandong Wu, Miao Ye and Zaigui Wang*
...: for adsorption, sample concentration was 2.542 mg·mL-1, pH was 3.7, processing volume was 4 BV; flow rate was 2 BV·h-1; temperature was 25°C. For desorption, elution solvent ethanol-water (95%, v/v) solution was 4.5 BV; flow rate was 2.0 BV·h-1. Flavonoids with content of 68.3% were obtained with a flavonoid recovery of 93.7%. The results showed that AB-8 resin revealed a good ability to purify total ...

 Zeenat Bano1,*, Sajid Abdullah1, Waqas Ahmad2, Muhammad Anjum Zia3 and Wardah Hassan1

... from all the sites. The concentrations of Zinc (Zn), copper (Cu) and nickel (Ni) were higher in river water as compared to farm and hatchery. Different organs of fish collected from different sites showed variations in their metal concentration. In Indus River fish, the concentrations of metals were highest in liver followed by kidney and gills. The Indus River fish also exhibited ...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regulated TFF3 mRNA expression. Furthermore, TFF3 mRNA expression was decreased by rhTFF3 treatment. These findings indicate that TFF3 attenuates LPS-induced cell damage, and TFF3 mRNA expression is influenced by LPS and rhTFF3 in intestinal mucous epithelial cell...

Bibi Haleema1*, Abdur Rab1 and Syed Asghar Hussain2 

... calcium, boron and zinc concentration for enhancing the growth and fruit related attributes of tomato. The experiment was conducted using Randomized Complete Block (RCB) Design with 3 factors, replicated 3 times. Calcium (0, 0.3, 0.6 and 0.9%), Boron (0, 0.25, 0.5%) and Zinc (0, 0.25, 0.5%) were applied as foliar spray three times. Calcium application at 0.6% increased plant height (88.04 cm), number of primary (2.63) and secondary (7.15) branches, leaves pla...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...rphology, viability, and concentration. In conclusion, gradation of electrical stimulation into three stages (our modified Method II) could help to ease the collection of semen samples from bulls with minimum discomfort signs. Furthermore, the modified method is also recommended to use for other animals, in particular, the wild animals.
...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
...8 using direct smear and concentration methods. One hundred and fifteen (11.04%) participants were found infected with one or more than one intestinal protozoans. Forty one (35.6%) of the participants were infected with single parasite and seventy four (64.3%) with multiple infections. Entamoeba histolytica 30.5% (n=77/252), Giardia lamblia 15.0% (n=38/252), Ascaris lumbricoides 17% (n=43/252), Trichuris trichura 11.1% (n=28/252), <...
Faiza Ambreen*1 and Muhammad Javed2
...and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated and fish were exposed to these concentrations, separately in glass aquaria for 70 days at constant laboratory conditions. Peripheral blood erythrocytes were taken on 14, 28, 42, 56 and 70 day of exposure for th...

Naila Riaz1 , Hafiz Muhammad Tahir2* , Khizar Samiullah3

...topically with different concentrations of the crude venom. All aphids died within 6 hr with oral treatment at 1.5µl of the venom dose. However, at the same venom dose, 100% mortality was achieved at 8 hr posttreatment when applied topically. Similarly, oral administration of 1µl and 0.5µl venom caused 100% mortality at 8 hr post-treatment compared to 10 hr and 12 hr post-treatment of topical application, respectively. It is concluded that or...

Irfan1* , Arshad Javid2 , Muhammad Altaf3 , Muhammad Shahbaz3 , and Khalid Javed Iqbal4 

...ignificantly higher urea concentrations 33.45±3.39 mg/dL were recorded from the serum of the birds reared under free range rearing system. However, non-significant variations in albumin, total protein, ALP and AST were recorded for all the three rearing systems. Significantly higher values of cholesterol, globulin, albumin, creatinine and ALT were recorded from serum of male M. gallopavo while urea and ALP were higher in hens’ serum. However, non-...
Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1
...hree levels such as NaOH concentration (0.6, 0.8, 1.0%), substrate concentration (5, 10, 15%) and residence time (4, 6, 8h) with and without steam (autoclaving at 121oC for 15min and 15psi). After pretreatment, cellulase production was conducted in 250ml Erlenmeyer flask at 50 ºC, pH 5 with shaking speed of 120 rpm using 2% pretreated eucalyptus leaves as carbon source and 2% v/v inoculum size in submerged fermentation ...

Ayesha Aihetasham1*, Khalid Zamir Rasib2, Syeda Rida Hasan1, Imran Bodlah3

...al efficacy of different concentrations of aqueous leaf extracts of three medicinal plants viz., Carica papaya (paw paw), Helianthus annus (Sunflower) and Bougainvillea glabra (Paper flower) against Heterotermes indicola. The leaf extract of C. papaya caused highest mortality i.e. 100% of 10%, 5% and 3% concentration. Bougainvillea glabra and H. annus caused 100% mortality at 10% and 5% concentration...
Muhammad Iqbal Anjum*, Shahbaz Javaid and Mukhtar Ahmad Nadeem
...icks on R-II (1.89). The concentration of Ca and P were 10.60, 9.47 and 11.43 mg/dL and 5.76, 4.91 and 7.11 mg/dL in blood serum and 32.61, 31.36 and 32.83% and 16.57, 15.92 and 16.60% in tibia bone of chicks fed rations I, II and III, respectively. The total feed cost per unit weight gain of broilers fed R-III diet was numerically 14% and 9% less than the broilers fed R-II and R-I, respectively. Results suggested that exogenous microbial phytase supplementati...

Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3
 

... conditions of 0.6% acid concentration, 10% substrate concentration, and resident time of 4h. The maximum FPase activity was 2.015 1U/ml/min under conditions of 0.8% acid concentration, 10% substrate concentration, and time of 6h. This showed that peanut shells could successfully be used as substrate for cellulase production in submerged fermentation.

 Maryum Fakhar, Iqra Jabbar, Anjum Nasim Sabri*

... presence of nickel. Two concentrations of nickel were used i.e., 500μg/ml and 1000μg/ml for subsequent tests. The results revealed that at low/ sub-inhibitory concentration of nickel (500 μg/ml), motility is inhibited; adhesion, hydrophobicity, aggregation, EPS production and biofilm formation of two strains are increased. This biofilm forming ability can be employed in
urification of waste waters and treat...

 Asma Akhtar, Abdul Rehman

.... The minimum inhibitory concentration of both isolates showed fair growth up to 1 mM of K2Te2O3. Both strains showed maximum growth at 37ºC and pH of 7. The bacterial growth in the presence of metal ions was delayed as compared to the control. High ellurite reduction potential was shown 98% (1f) and 97% (2b) after 24 h of incubation. Glucose 6 phosphate dehydrogenase (G6PDH) activity was increased to 279% (1f) and 150% (2b) when ompared to the respective...
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
.../sup> of LC50 concentrations of iron chloride for 30 days in the glass aquaria with three replications for each treatment. After 30-day exposure of iron chloride, the fish organs viz. kidney and liver were isolated and the peroxidase enzyme activity was analyzed. Activity of peroxidase was assessed by measuring the conversion of guaiacol to tetraguaiacol, spectrophotometrically, at a wavelength of 470 nm. The peroxidase activity was measured ...

Farah Rauf Shakoori, Momina Shokat, Faiza Saleem and Tanzeela Riaz

...6;C, pH 9 using 1% xylan concentration, while XPB-CW01 produced maximum amount of xylanase at 50ᵒC, pH 7 with 1.5% xylan concentration. Maximum xylanase activity was achieved at temperature 50ᵒC, pH9 with 2% xylan concentration. Xylanase produced by XPB-GS02 was active from 35-80oC, while XPB-CW01 was active from 30 _70oC with optimum temperature 50°C.

...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
...8 using direct smear and concentration methods. Two hundred and twenty one (21.2%) participants were found infected with one or more than one intestinal tapeworms. Seventy seven (7.39%) of the participants were infected with single parasite and one hundred forty four (13.8%) with multiple infections. Taenia saginata 32.6% (n=146/447), Ascaris lumbricoides 20.3% (n=91/447), Hymenolepis nana 19.7% (n=88/447), Trichuris trichura 14.3% ...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

...plemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots ...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...wed decay in mean lutein concentration at higher temperatures i.e. 25oC and 45oC. The microbiological study of the extracted color and candies prepared in laboratory and dyed with T. erecta extract color showed that the color extracted from T.erecta itself do not promote microbial growth rather it has antibacterial activity. The lethal dose and toxicity determination of the color extracted from marigold showed that it is saf...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
...th ammunium sulphate, at concentrations as low as 30 μl. Optimized conditions for maximum yield of mutant SK included CSL 6%, 24 h. of fermentation, pH 7.5, 37oC, KH2PO4 0.04%, K2HPO4, NaHCO3 0.15% and 0.015% CH3COONa. The results indicated that UV is the most effective mutagen for hyper-production of SK. Mutant strain UV6 has the potential for enhanced SK production with commercia...

Ata-ul-Mohsin*, Ehsan-ul-Haq** and Muhammad Naeemullah*

...cium and high phosphorus concentrations applied to a partially resistant wheat cultivar “Rapier” did not have a significant effect on the growth and development of the aphid or its parasitoid. However, the mean fecundity of the parasitoid was higher on plants with high phosphorus than those treated with high calcium concentrations.

...

 Nasreen Sultana* and A.Ghaffar**

...cides at three different concentration viz., 1, 2, 3 g (a.i.) kg-1 seeds reduced the recovery of the seed borne fungi. The most effective seed treatments were Benlate, Topsin-M, Carbendazim and Aliette @ 3 g kg-1 seeds which enhanced seed germination and reduced seed infection in bottle gourd. Trichoderma harzianum, T. viride, Gliocladium virens, Stachybotrys atra and Bacillus subtilis showed better results in the control of pre- and post-emergence infection o...

 Ahmed Aziz Kurd, Amanullah, Saifullah Khan, Basharat Hussain Shah and Munir Ahmed Khetran*

...eased with increased IBA concentration, whereas, number of roots and root length increased with increased IBA concentration. It was concluded that to obtain the most roots per cutting and the longest root length, application of 4000 ppm IBA was the best and to obtain the most number of rooted cuttings, application of 3000 ppm IBA was the best.

...

 Rahila Nazli, Tehmina Sohail, Bushra Nawab and Zahra Yaqeen*

...idia sepium in different concentration was used to investigate its antimicrobial activity against gram +ve and gram-ve bacteria. The study was also extended to some species of fungi. Results showed that the activity was more pronounced against gram +ve organisms and fungi. Maximum inhibition activity was calculated against all the groups of organisms, which was between 0.5 and 1mg ml-1 against bacteria and 2.5 mg ml-1 against fungi. It is therefore concluded t...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...ded among all groups and concentration of red blood cells (RBCs), white blood cells (WBCs), hematocrit (HCT) and numbers of lymphocytes were highest in treatment # 1 with the mean values of 1.29×10ˆ12 L-1, 54.9×10ˆ9 L-1, 18% and 48.7×10ˆ9 L-1. Microbial load was also determined inside and outside of fish as highest microbial load was observed in control group fish on both media nutrient agar (NA...
Abul Hassan Faiz*, Fakhar-i-Abbas and Lariab Zahra Faiz
...otassium and phosphorous concentration. These significant differences in soil composition have long-term effects on ecosystem sustainability and productivity of vegetation.
...
Jaime Molina-Ochoa1, 2, 6,*, Edelmira Galindo-Velasco2, Ana María Rosales-Gutiérrez2, Martín González-Ramírez3, Roberto Lezama-Gutiérrez3, Wilberth Chan-Cupul3, Steven R. Skoda4, Muhammad Irfan Ullah5, Luis Jorge García-Márquez2 and  John E. Foster6
...pers. For each treatment concentration, 10 adult engorged ticks were placed in the Petri dishes, and each treatment was replicated four times; the experiment was replicated two times. Tick mortality was recorded daily for 9 days. Our EPNs were able to parasitize engorged ticks, and tick mortality ranged between 70-100% after 9-d post-exposure. The faster and higher tick mortality was obtained with the concentration of 2000 I...

 Arshad Ali, Muhammad Arshadullah, Syed Ishtiaq Hyder, Imdad Ali Mahmood and Badar-uz-Zaman*

RICE PRODUCTIVITY AND SOIL HEALTH AS AFFECTED BY WHEAT RESIDUE INCORPORATION ALONG WITH NITROGEN STARTER DOSE UNDER SALT-AFFECTED SOIL
...yzed for K, Ca and Na -1 concentration in grain and straw. Tillering, grains spike , 1000-grain weight and paddy yield significantly (P≤ 0.05) increased with different -1 levels of N doses. Maximum tillers plant (27.66), plant height (138.33 cm), -1 spike length (28.33 cm), and grains spike (175), 1000-grain weight (28.66 -1 -1 g) and paddy yield (4.25 t ha ) was harvested with 5 t ha wheat straw -1 incorporation plus 60 kg N ha . The paddy yield was 19% mo...

 Syed Ishtiaq Hyder, Muhammad Arshadullah, Arshad Ali and Imdad Ali Mahmood*

EFFECT OF BORON NUTRITION ON PADDY YIELD UNDER SALINESODIC SOILS
... growth, yield and ionic concentration of rice directly sown on raised beds under -1 saline sodic soils (ECe=5.32 dS m , pH=8.52 and SAR=18.87) at Soil Salinity Research Institute, Pindi Bhattian, district Hafizabad, Punjab Pakistan during 2009 and 2010. Treatments were arranged in RCBD with three replications. The crop was harvested at maturity. Data on tillering, -1 plant height, spike length, number of grains spike , 1000-grain weight, straw and paddy yield...
EVALUATION OF LEGUME HERBS NUTRITIVE VALUE AS A RUMINANT FEED AND NITROGEN SUPPLY ON SOIL IN WEST TIMOR, INDONESIA
...d that the highest -l NO concentration after planting was in CP (5.72 mg ), followed by CT 3 -l -l -l (4.21mg ), MB (3.44 mg ), and DL (2.83 mg ). The species of legume was obviously highly significant (P<0.01) to biomass production 90 days after planting. Moreover, dry matter (DM) and organic matter (OM) in vitro digestibility were statistically different among the species of legume (P<0.01). In DL the DM and OM were highest i.e., 74.84 % and 74.37%. Me...

 Arshad Ali, Imdad Ali Mahmood, Muhammad Salim, Muhammad Arshadullah* and Abdul Rasool Naseem**

GROWTH AND YIELD OF DIFFERENT BRASSICA GENOTYPES UNDER SALINE SODIC CONDITIONS
... of crop maturity. Ionic concentration in plant tissues and oil content in seeds were also determined. Comparatively more number of branches and pods per plant were produced by cultivar Dunkled closely followed by BARD-I while maximum seed yield (241.7 and -1 235.1 kg ha ) was obtained from Dunkled and Sultan Raya, respectively which was statistically at par. However, BRS-II and Rainbow showed significantly more percent oil contents in their seeds but genotype...

 Muhammad Arshadullah, Syed Ishtiaq Hyder, Arshad Ali and Imdad Ali Mahmood*

CUMULATIVE EFFECT OF SULFUR AND CALCIUM ON WHEAT GROWTH AND YIELD UNDER SALINE-SODIC SOILS
...psum on growth and ionic concentration of -1 wheat variety (Saher) sown in saline-sodic soil (ECe=5.32 dS m , pH=8.52 and SAR=18.87) at Soil Salinity Research Institute (SSRI) Farm, Pindi Bhattian during rabi 2009-10. Treatments were arranged using randomized complete block design (RCBD) with three replications. The crop was harvested at maturity, data on tillering, plant height, spike -1 length, number of grains spike , 1000-grain weight, straw and paddy yiel...

 Abdul Rehman Roonjho*, Waseem A. Gillani**, Awais Rasool**, Naheed Akhtar**, Tariq Mahmood**, Arsalan A.***, M. Afzal*, Iqbal Khan* , M. Asghar Ranjha**** , M. Irfan**** and Javed Khan**

REPELLENCY EFFECTS OF DIFFERENT PLANT EXTRACTS TO COTTON MEALY BUG, TINSLEY (HEMIPTERA: PSEUDOCOCCIDAE)
...ol were evaluated at the concentration of 1000, 500 and 250 ppm against cotton mealy bug ( ) in a free choice bioassay for two weeks. The ethanol extract of was the most effective against cotton mealy bug having highest repellency (72.5%) at 500 ppm dose. The lowest average repellency (26.3 %) was observed in acetone extract of at 500 ppm dose.

...

 Adnan Umair*, Safdar Ali**, Muhammad Sarwar***, Kashif Bashir**, Muhammad Javed Tareen**** and Muhammad Asghar Malik*****

ASSESSMENT OF SOME PRIMING TECHNIQUES IN MUNGBEAN (VIGNA RADIATA) : A GREEN HOUSE STUDY
...lso improved the protein concentration at the early stage of seedlings. Phosphorous application through priming significantly improved germination up to 95%, seedling -1 vigour index up to 23.05 and protein content up to 2.17 (g FW).

...

 Said Salman*, Shah Jehan Khan*, Javed Khan**, Rehmat Ullah Khan*** and Imran Khan****

GENETIC VARIABILITY STUDIES IN BREAD WHEAT (TRITICUM AESTIVUM L.) ACCESSIONS
...eters except chlorophyll concentration index and number of spikelet per spike indicating considerable amount of variation among the accessions for each trait. The genotypic and phenotypic coefficient of variation estimates were higher for all the traits except chlorophyll concentration index and days to physiological maturity. Highest heritability estimates and expected genetic advance were found for all the traits except ch...

 Nazakat Nawaz*, Malik Shah Nawaz*, Mubashir Ahmad Khan*, Mirza M. Yasin*, Doulat Baig*, Nasir Mehmood Cheema**, Muhammad Amjad** and M. Altaf Sher**

EFFECT OF BORON ON PEANUT GENOTYPES UNDER RAINFED CONDITIONS
...equirements and critical concentrations in diagnostic parts of three peanut genotypes, BARD-479, ICG-7326 and ICGV-92023. These genotypes were grown in boron deficient soil at National Agricultural Research -1 Centre, Islamabad, Pakistan. Five rates 0.0, 0.5, 1.0, 2.0 and 4.0 kg B ha as boric acid (H BO ) were applied. Boron fertilizer applications significantly 3 3 increased dry pod yield of all the tested genotypes over control. Dry pod yields of BARD-479, I...

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...etween strains and among concentrations. Abamectin, at LC and LC , changed the activities 10 20 /levels of TE, CE, AcP, total protein and FAA in the larvae of both the strains. The activities of alpha-amylase, glucoamylase and AkP remained non-significant at both doses in the two strains. In PAK strain larvae, the TE activity was inhibited with depletion of total protein contents and elevation of FAA contents. In FSS-II larvae, the effect of abamectin on level...

Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***

ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...s were cytotoxic. As the concentration decreased, cytotoxicity as well as antiviral activities were lost. Based on these findings, it was concluded that GZ-08™ sanitizer or spray can be used as antiviral agent to clean or disinfect some non-living surfaces against different viruses in general and NDV in particular. However, in-vivo use of GZ-08™ in poultry against NDV is recommended only as pre-treatment with ND vaccines as it significantly reduced...

 Azhar Usman Ali*, Ghulam Sarwar*, Muhammad Aftab* and Sher Muhammad**

EFFECT OF SOIL AND FOLIAR APPLIED COPPER ON GROWTH AND YIELD OF WHEAT (TRITICUM AESTIVUM L.)
... and analyzed for copper concentration by using wet digestion method. Results of the experiment indicated that soil application of copper proved better than foliar application in increasing the yield and yield components of wheat. Similarly, foliar application -1 -1 of copper at 4mg kg (T ; level 2) was better than 2 mg kg (T ; level 1). 

...

 Muhammad Mudasar Aslam ⃰, Muhammad Jamil**, Ijaz Malook**, Amana Khatoon*, Ali Rehman*, Abdur Rahim***, Pirzada Khan*, Shakir Ullah Khan Shakir*, Shahid Irfan*, Faizan Ullah****, Khair Ul Bashar**, Mahideen Afridi** and Shafiq Ur Rehman*

PHYTOTOXIC EFFECTS OF CALOTROPIS PROCERA, TAMARIX APHYLLA AND PEGANUM HARMALA ON PLANT GROWTH OF WHEAT AND MUSTARD
... aqueous extracts at all concentrations (5%, 10%, 15%, 20% and 25%) but shoots were inhibited by higher concentrations (20% and 25%). This study revealed that wheat is more sensitive to different plant extracts as compared to mustard. It is thus concluded that inhibitory effect increases with the increase of extracts concentration.

...

 Abdul Rehman Khan*, Muhammad Mahmood–Ul-Hassan**, Rizwan Ahmad** and Anjum Munir ***

ARSENIC III TOLERANCE POTENTIAL OF FUNGI ISOLATED FROM POLLUTED AND NON-POLLUTED SOILS OF PAKISTAN
...m amended with different concentrations of As (50 to 5600 mg/L). The fungal tolerance was evaluated by comparing tolerance index (TI), minimum inhibitory concentrations (MIC) and growth rate. Out of 18 isolates, 12 belonged to genus , 3 to , 2 to and one to . The isolates and appeared to be most tolerant to arsenic. Fungal strains isolated from Gujranwala soil exhibited more As(III) tolerance than those from Multan and Islam...

 Asif ullah Khan*, Faizan Ullah*, Sultan Mehmood*, Muhammad Irshad* and Farhat Ullah Khan**

ALLELOPATHIC EFFECTS OF JATROPHA CURCAS L. LEAF AQUEOUS EXTRACT ON EARLY SEEDLING GROWTH OF L. PARTHENIUM HYSTEROPHORUS
.../ gm of extract). Higher concentration of the extract (25%) significantly decreased seed germination (%), seed germination index, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, seedling relative water content and seed vigor index. It was inferred from findings of the present investigation that aqueous extract of exhibits inhibitory effects on growth and development of and therefore could be a potential source of botanica...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
..., Huwa-San TR50 caused a concentration dependent reduction in the percentage of egg hatching. The percentages of larvae hatching from eggs of A. lycopersici were 73.42, 56.92, 25.41 and 17.97 under greenhouse conditions and 68, 53.14, 23.51 and 15.18 under laboratory conditions at 1000, 2000, 3000 and 4000ppm of Huwa-San TR50 after one week of treatment compared with the control (well water), respectively. The application of Huwa-San TR50 showed a promi...
Filiz Kutluyer* 
...tative changes different concentrations of L-tryptophan. In addition, L-tryptophan can be used in activation medium for O. mykiss, S. rizeensis and S. coruhensis.
...
Fatmagün Aydin1,* and Fatma Burcu Harmantepe2
...d cell count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total white blood cell count and differential leukocyte counts) showed no significant (P>0.05) differences between the control and all other treatments. Feed intake, specific growth rate, weight gain and final weight were decreased significantly with the increase of sage oil ...
Asmatullah1, Chaman Ara1,*, Shagufta Andleeb2, Maha Tahir1, Beenish Zahid1 and Madeeha Arshad1
...rpose, different aqueous concentrations of cadmium chloride i.e. 0.00, 6.25, 12.50, 25.00 µg/g B.W of mice were given orally to pregnant mice on days 6-12 of gestation daily. Another series of mice were treated with different concentrations of cadmium chloride and also with an antidote (guava fruit extract) on days 6-18 of gestation daily once a day. Fetuses were recovered on day 18 of gestation, analyzed based ...
Hao Zhang,1,2,3,* Hai Tao Nie,3 Tie Wei Ma,3 Zi Yu Wang3 and Feng Wang3
... to estimate the mineral concentrations and distributions in the main body tissues and the net requirements for maintenance and growth of F1Dorper × Hu ewe lambs. Thirty-five ewe lambs averaging 33.52±0.56 kg body weight (BW) were used. Seven ewe lambs were randomly chosen and slaughtered at 34.93 ± 0.37 kg BW as the baseline group for measuring initial body composition. Another seven lambs were also randomly chosen and offered a...
Hongwei Liang1,2,*, Miaomiao Tong1, Lihuan Cao1,2, Xiang Li3, Zhong Li1 and Guiwei Zou1,*
...red. Calipash had higher concentrations of flavor amino acids than muscle. Muscle tissue of the Huaihe River strain had the highest concentration of pharmacodynamic amino acids and the highest concentration (P < 0.05) of arachidonic acid (females: 109.83 mg/ 100g, males: 110.72 mg/100 g). Concentrations of eicosapentaenoic acid and docosahexaen...
Shireen Ihsan Izzaddeen1 and Ali Kaygısız2,*
...albumin, GH and globulin concentrations did not differ between the groups (P>0.05). Essential laurel oil containing whole milk, on the other hand, lowered serum triglyceride concentration (P<0.05). Altogether these results suggested that the consumption of laurel essential oil (600 mg/day) containing whole milk by calves might have positive influences on their health parameters compared to the control group.
...
Nilgün Paksoy1, Hikmet Dinç2 and Serap Kılıç Altun3,*
...udy was to determine the concentrations of Na, Mg, P, K, Ca, Fe, Cu, Zn, Ni, Cd, V and Ba in donkey, goat, and sheep milk samples. Fifty-six individual milk samples were collected from 17 lactating donkeys, 19 goats, and 20 sheeps in three different Turkish Farms. The samples were analyzed by inductively coupled plasma - mass spectrometry (ICP-MS). Minimum and maximum levels of Na, Mg, P, K, Ca, Fe, Cu, Zn in donkey milk samples were 108.6-202.7, 48.6-76, 355-...

Muhammad Jamal Khan1, Abdul Malik2*, Muhammad Shahzad Khattak2, Naveedullah1, Naeem Ijaz3 and Ishaq Ahmad4 

...linity having low Sodium concentration and may be suitable to salt tolerant crop. Regular assessment of water resource and constant monitoring of water quality is recommended. An appropriate measure may be adopted for recharge of the ground water aquifer through development of small dams in the catchments plain. 

...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
... optimal Co2+ concentration for the maximal activity of AMYSBS. Presence of ionic (SDS) and nonionic (Tween-20, TritonX-100) detergents showed an enhancing effect on the activity of AMYSBS. Stability studies of AMYSBS exhibited that enzyme was quiet stable at 55 °C. Kinetic studies demonstrated the Km and Vmax values of 6.67mg/ml and 2500µmol min-1 mg-1, respectiv...

Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4 

...ose, cortisol and HSP-70 concentrations wasmaximum in HF (43.44, 119.06 and72.11%) andcrossbred (38.66, 159.61 and79.12%) and minimum inAchai (34.93, 123.59 and52.53%) and Sahiwal (27.26, 117.22 and65.41%)respectively (P< 0.01). Holstein Frisian appeared as the most heat sensitive breed exhibiting changes at 32 °C i.e. 5 °C higher than 27 °C (upper critical temperature, UCT). Sahiwal and Achai were the most heat tolerant breeds manifesting chang...

Nadia Mubarik1, Aqib Iqbal1*, Iqbal Munir1 and Muhammad Arif2 

... water stress) and three concentrations of CaCl2 (5, 10 and 15 mM CaCl2.2H2O) as foliar spray on maize seedlings 20 days after emergence (DAE). Water was sprayed as untreated control on maize seedlings 20 DAE. After foliar application, the plants were regularly irrigated upto one week and water stress was imposed by withholding water from half of the pots for 20 days. Data were recorded on the relative water content (RWC), proline, sugar content and transcript...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...nificantly increased the concentration of tumor necrosis factor-α (TNF-α) in bursa of Fabricius (P<0.05), along with an increase in the concentrations of immunoglobulin-G (IgG) and interleukin-12 (IL-12) in the serum of ducks at 1 day and 7 days after LPS injection (P<0.05). However, there were no apparent changes in organ index or the relative expression of TLR4 and IgM (P>0.05). Fu...
Sherin Reda Rouby
...ds that reflect the high concentrations of the virus. The results of the current study confirm the suitability of RP030 gene in differentiation of lumpy skin disease virus and sheep poxvirus in a single PCR assay without the necessity of post processing steps. 
...
Shagufta Andleeb*1, Fariha Zahid1, Noor-ul-Ain1, Yousra Nisar1, Chaman Ara2
...mesticus). Different concentrations of permethrin (0, 5, 10 and 20ppm) were prepared in sterilized distilled water. These doses were injected into eggs on 3rd day while recovery of embryos was done on 7th day of incubation. Disrupted embryos with microcephly, hydrocephaly, short neck, micromelia, amelia, micrognathia, agnathia, cataract, ectopiacordis, omphalocoel, axis distortion, anencephaly, anophthalmia, microphthalmia, phocomelia...
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
...evels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase production was achieved by solid substrate which was conducted in 250mL capacity Erlenmeyer flask in which incubation of Bacillus subtilis was carried out for 24 h of fermentation period at temperature of 50oC. Results showed that cellulase production was greatly affected by ...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
... SFF supplemented at 10% concentration showed a significant increase in blastocyst development when compared with other groups. Gene expression analysis revealed a significant increase in BAX expression in groups supplemented with SFF 20% and 40%, whereas the expression of BCL-2 was significantly increased only in the 40% SFF supplemented group. The ratio of Bax to Bcl-2 was significantly increased in the embryos derived from oocytes matured in 20% and 40% SFF...
Felicia NkechiEkeh*, Ifeanyi Oscar Ndimkaoha Aguzie, Joy Ihuoma Nzei, Chinenye Maria-Goretti Ohanu, Godwin Ikechukwu Ngwu and ChukwudiebubeUgolo
...wo extracts in different concentrations, a total of 63 sundried adult C. gariepinus of mean weight 111.47 ± 2.34g were randomized into three treatment groups A – C for each extract and a control D. The treatment groups were in triplicates with each containing three catfish. The groups contained fish infested with 20 larvae, 20 adults and eggs of D. maculatus. Groups A – C were exposed to 8, 12 and 15% ( w/w) of extract per kilo...
Hafiz Azhar Ali Khan1,*, Waseem Akram2, Sumi Lee3, Shakir Manzoor1, Syed Rajab Ayub1, Khalil Ur Rehman1, Shinawar Waseem Ali1, Muhammad Bilal Chattha1 and Sumaira Maqsood1
.... Based on median lethal concentration (LC50) values, all the strains of S. oryzae and R. dominica were more susceptible to spinosad than T. castaneum strains. The resistance ratios (RRs) of field strains at LC50 values were in the range of 2.24 to 3.24 fold for T. castaneum, 3.33 to 9.00 fold for R. dominica, and 1.73 to 3.45 fold for S. oryzae. The results revealed a variation in susceptibility ...

Feroza Haider1,2, Shamim Gul2,3*, Javaid Hussain4, Sadaf Asalam Ghori5, Muhammad Naeem Shahwani6, Muhammad Murad6 and Abdul Manan Kakar

...educed significantly the concentration of iron and zinc in roots of B. rapa. 

...

Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Muhammad Jamal Khan1, Zahir Shah1 and Farmanullah Khan1 

...various nutrients uptake/concentration. The data revealed that all strains of cyanobacteria had significantly (p < 0.05) improved the nutrients up-take/concentration and agronomic traits of rice crop as compared to control. Among the strains, the Gloeobacter strain (MMF-4) surpassed by augmenting all agronomic parameters of rice crop like plant height (85 cm), plant fresh weight (45 g), dry weight (24.5 g) and root dry we...

Gulsanga1, Zahin Anjum1, Sahib Alam2* and Farhat Shehzad

...d that Cu, Zn, Pb and Ni concentrations in cow meat ranged from 7.27 to 14.81, 64.00 to 112.12, 3.30 to 6.25 and 0.73 to 2.53 mg/kg whereas the concentrations of these heavy metals in buffalo meat varied from 3.15 to 7.71, 72.88 to 88.47, 1.41 to 13.28 and 0.38 to 1.93 mg/kg, respectively. The average Na, K, Ca, Mg and P contents in cow’s and buffalo’s meat were found to be 54.75 and 67.45, 351.67 and 333.75, 4.6...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
... IPTG induction to final concentration 0.5 mM at 37oC for 4 h and purified using affinity chromatography. SUMO tag was removed from SUMO-IFNα2-Tα1 by SUMO protease and recombinant IFNα2-Tα1 was collected in flow through by affinity chromatography. The MW (~23 kDa) of IFNα2-Tα1 was determined by 12 % SDS-PAGE and its integrity was confirmed by Immuno blot analysis using anti-interferon α-2 and anti-thymosin &...
Huma Naz1,*, Sajid Abdullah2, Sidra Naz3, Sidra Abbas2, Wardah Hassan2, Shakeela Perveen2, Moazama Batool1, Laiba Shafique2
...50 and lethal concentration of Fe+Ni+Pb+Zn mixture for the fish, Cirrhina mrigala was determined in this research work. The fish were exposed to each of the 19 different concentrations (5-90 mg/L) of Fe+Zn+Pb+Ni mixture to determine 96 h LC50 and lethal concentration. During acute toxicity test, behavioral changes and catalase activity in liver, kidney, gills, ...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...olism and macromolecular concentrations of 4th and 6th instar larvae of a stored product pest, Trogoderma granarium. The LC50 values of λ-cyhalothrin for 4th and 6th instar larvae of Lahore population was 15.93 and 13.76ppm respectively, while 19.07 and 16.21ppm were for the 4th and 6th instar larvae of Gujranwala population, respectively. The resistance ratio RR (0.67 a...

Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1* 

...The influence of varying concentrations of both EOs was determined in vitro using the agar dilution test. Concentration as low as 0.25% and 0.125% of thyme and clove oils, respectively, were able to completely inhibit the growth of CP. An in vivo study was conducted in commercial broiler chickens, where, 2 groups of 10 day-old broilers were fed a balanced ration with addition of thyme (0.25%) or clove (0.125%). Birds were th...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
...n. In the present study, concentration levels of seven heavy metals were measured in different body parts of Baladi chicken from Jazan region, Saudi Arabia using Inductively Coupled-Plasma Optical Emission Spectrometry (ICP-OES). The concentrations of heavy metals in various chicken parts were recorded to be in the range of nd-0.01, 0.07-0.18, 1.08-3.95, 9.61-119.0, nd-0.16, 0.13-1.10, and 8.63-67.58 mg/kg for Cd, Cr, Cu, Fe...
Wael S. El-Tohamy1,*, Russell R. Hopcroft2 and Nagwa E.M. Abdel Aziz3
..., temperature, phosphate concentration and phytoplankton biomass. The main spatial gradients along the estuary were associated with salinity. The high salinity zone in the estuary downstream was dominated by the calanoid paracalanidae and the harpacticoid Euterpina acutifrons. In the lower salinity transects, the tintinnid Favella serrata dominated the estuary midstream, together with several rotifer species in the estuary upstream. The juvenile ...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
... higher serum GH and PRL concentration in comparison with T2 and control groups (p < 0.05). Expectantly, mice in T1 group also showed significantly higher average weight gain and offspring weaning weight than that in T2 and control groups, respectively (p < 0.05). Overall, these results suggested that novel somatostatin plasmid fused to tPA signal peptide obtained stronger immunogenicity, and better potentials of promoting growth and...
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
... to determine the lethal concentrations of this virus against the first and second larval instar of S. exigua. The potency of ten plant extracts as UV absorbers were done by measuring the absorption spectra of the 0.5% extracts using UV spectrophotometer. Based on preliminary data, clove, green tea and henna extracts at 10% (v/v) concentration were added to the virus and tested under semi-field conditions on the cabba...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...y treated with different concentration of EMS and MMS. Among 13 mutant variants, EM-7 showing maximum lipase activity of 71±3.55 U/g, was selected. Resistance in the hyper producing strain was developed by exposing it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optimum conditions at 30°C temperature, pH 7, and incubation period 64 h, the lipase activity was increased up to 124±2.48 U/g. The cru...

Wasim Javaid1, Farid Asif Shaheen1*, Farah Naz2 and Muhammad Usman Raja2 

...y using different fungal concentrations of 1×106, 1×107 and 1×108 spores/ml in stored chickpea grains. Highest mortality (90%) of PB was observed in 72 hours through application of 1×108spores/ml of M. anisopliae compared to B. bassiana with 60% mortality. On 24 hours of exposure, minimum mortality (4%) in PB was recorded in grains treated with 1×106 spores/ml of B. bassiana. In M. anisopliae, the concentr...
Muhammad Anwar Iqbal1, Habib Rehman2, Ali Hussain3,*, Faiza Jabeen4, Ijaz Ahmad2, Kamran Ashraf5, Muhammad Irshad Arshad6 and Omaima Khan7
...ineral levels and caecal concentrations of E. coli and Clostridium spp. in Japanese quail (Coturnix coturnix japonica). Japanese quail (one-day old; n = 1320) were taken and randomly separated into four groups (n = 320), having 8 replicates (n = 40) in each group. Corn-based poultry feed (basal diet) was given to control group birds (Group A) and the same feed mixed with three different concentrations of...
S. Jalbani1, N. T. Narejo2,*, P. Khan2 and Y.M. Jalbani3
...ocytes count, hemoglobin concentration and erythrocyte sedimentation rate of the two sexes. Male fish had total erythrocytes 5.05 ± 0.5 × 106 and hemoglobin content 11.5 ± 1.5 g/in ml compared to 4.04 ± 0.5 × 106 and 7.3 ± 1.3 g/in ml in the female. The female fish had significantly higher values of ESR in comparison of male. It may be due to different plasma viscosity and specific gravity of the er...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
... mice fetuses. Different concentrations of the drug, 0.00, 4.10, 8.33 and 16.66 µg/g B.W. were administered orally to the dams on days 6-12 of gestation, and fetuses were recovered on day 18. Morphological observations of fetuses from 4.10, 8.33 and 16.66 µg/g B.W. dose groups showed abnormalities such as skin hemorrhages, microphthalmia, limb deformities, hygromas, kyphosis, curved and short tail and fluid filled abdominal cysts. Significant incre...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
...t in the camel milk. The concentration of immunoglobin was found higher in camel and goat milks as compared to other species. Moreover, the strength and mobility of bovine serum albumin (BSA) was different in all milk species.
...

Abdur Rehman and Zahir Shah 

...t of nodules by 135 %, N concentration in plant by 29 %, N uptake by 50 %, N2 fixation by 85 % over the control. Application of Mo and Fe at all levels alone or in combinations also improved soil organic matter, mineral N, P and K status that could be associated with more N2 fixation, enhanced plant and root growths of plants. These results suggested that application of both Mo and Fe is necessary for pea crop enhanced nodulation, N2 fixation, yield, nutrient ...
Kamran Khan1, Sarzamin Khan2, Nazir Ahmad Khan2,* and Naseer Ahmad2
...antify the effect of two concentrations and technological forms of flaxseed supplementation on growth performance, carcass yield and meat quality of rabbits. Sixty indigenous rabbits were divided randomly into five treatment groups. The control group was fed on standard concentrate basal diet, whereas in the treated groups, the basal diet was supplemented with low (3.5%; LCF) or high (7%; HCF) level of crushed flaxseed and low (1.5%; LFO) or high (3%; HFO) lev...

Fiaz Ahmad1, Mumtaz Hussain2, Ghulam Abbas1, Muhammad Jawad Asghar1 and Muhammad Rizwan3* 

... to three different Pb2+ concentrations under two water stress conditions. Different morpho-physiological and yield traits were studied. Both varieties showed gradual reduction in morphological traits at increasing concentration of lead and water stress. Moreover, morphological traits were more severely affected by combined lead and water stress treatment (80 mg Pb2+ 50% FC) as compared to single lead (40 mg Pb2+ 100% FC and...
Md. Abu Affan1,*, Abdulmohsin Al-Sofyani1, Adnan Jaman Turki2 and Mohammed S.B. Hariri3
...inity and nearly zero DO concentration might be the reasons of killing fish by creating osmotically imbalance and suffocation of fishes. Additionally, due to low pH, huge mucous covered the gills filaments which might have created difficulties for respiration. Thus, the fish killing incident might have occurred by synergetic effects of different physico-chemical factors.
...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...nc and ferric oxide at a concentration of 12, 200 and 1µg/ml, respectively. This study may help in the development of chemotherapeutic methods against multidrug resistant bacterial pathogens in chronic and acute lung infections. It provides a practical approach towards the use of nanoparticles to enhance antimicrobial activity against these pathogens.
...
Habib-ur-Rehman1*, Saima Mirza1*, Mansoor-ul-Hasan2, Qurban Ali3, Hafiz Abdullah Shakir4, Muhammad Yasir5
...th micropipette at three concentrations (5, 10 and15%) of each of the plant extract. The repellence was recorded after 24, 48 and 72 h of the treatments application. The findings of experimental trials presented significant mean repellency 79.15% of T. castaneum (with methanolic extract of C. paradise)followed by 76.21% (with R. communis extract)and 63.36% (in J.curcus extract) at 15% concentra...
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...olism and macromolecular concentrations of 4th and 6th instar larvae of a stored grain pest, Trogoderma granarium. The LC50 values of esfenvalerate for 4th and 6th instar larvae of Phosphine and deltamethrin-susceptible population was 34.29 and 28.05ppm, respectively, while 39.30 and 32.67ppm were for the 4th and 6th instar larvae of Phosphine and deltamethrin-tolerant populati...

Muhammad Zahid1, Naeem Iqbal1, Sohaib Muhammad2*, Summiya Faisal3, Wajid Mahboob3, Makhdoom Hussain4 and Zaheer ud din Khan2 

... was supplied in various concentrations (0, 5, 10, 25 and 50 mM) as seed priming and foliar spray treatments at vegetative and reproductive stages. Plant growth and grain yields were reduced under drought. Two glucose treatments (10 mM and 50 mM) improved tillering and dry biomass as compared to other treatments. Foliar sprayed-glucose treated plants showed increasing trends in grain yield under drought. Physio-chemical attributes were also modulated by exogen...
Nevran Eylem Akman Gündüz1,* and Özgür Özcan2
...oration assay. Different concentrations (2, 5, 10, 50, 100, 200, 500 and 1000 mg/L) of GA3 were added to the synthetic diet and protein, lipid, carbohydrate and glycogen levels in the hemolymph were evaluated for the GA3 concentrations. The hemolymph protein level of the larvae increased significantly at 5, 10, 50 and 200 mg/L with respect to the control group. The lipid level of hemolymph fluctuated am...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...ied by testing different concentrations (1 x 102 POB (Polyhedral occlusion bodies) mL-1- 1 x 1010 POB mL-1) from the occlusion bodies produced from NPV isolates against 2nd, 3rd, 4th and 5th instar larvae of S. litura. The rapid and sensitive polymerase chain reaction (PCR) technique was used for the molecular detection of NPV gene from native NPV diseased insect. Mu...
Xiaqing Chen, Yü Huang and Zhen Huang*
...azim was affected by the concentration of each component in the mixtures. The synergistic effects were observed in treatments T4 (3.5+40.0), T5 (7.0+20.0) and T6 (7.0+40.0), respectively. The treatment T3 (3.5+20.0) was determined as additive effects according to their cumulative adjusted mortalities, Me and Chi-square values. The current research on the joint action of ethylacetate extracts from T. hamatum with carbendazim offered a promising, safe and...
Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
... directly related to the concentration of each component used in mixtures. Additive effects were observed in all treatments according to the mycelial growth inhibition, Me and Chi-square values. The use of T. hamatum offers a promising and effective co-formulation product or alternative to chemical fungicides in controlling popcorn disease of mulberry.
...

Ali Sher1*, Khalid Naveed1, Gulzar Ahmad2, Ayub Khan1, Muhammad Saeed1 and Shah Masaud1 

... and 150 kg ha-1), three concentrations of Zn and Fe (i.e. 1, 2, 3 kg ha-1) each along with two controls i.e. no micro nutrients no water (control-I) and no micro nutrients + water (control-II). The N was soil applied and MN as foliar spray at booting stage. Days to anthesis, leaf area tiller-1, leaf area index, plant height, days to physiological maturity and biological yield were significantly increased by both N and Zn while the same parameters except days ...

Safina Naz1, Muhammad Akbar Anjum1, Saeed Akhtar2, Syed Atif Hasan Naqvi3* and Muhammad Asif Zulfiqar

...sewage water had greater concentrations of these metals, which were higher than the safe limits recommended by WHO (1996). All the vegetables grown with tubewell water had significantly lower heavy metal contents, well below the critical limits. The concentration of Pb in tomato and cauliflower, Cd in tomato and spinach, and Fe in spinach irrigated with canal water were also detected beyond the safe limits. The study conclud...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
...re exposed to sub lethal concentrations of deltamethrine. Fish were divided into 4 groups and each group contains six fish. Experimental groups viz. A, B and C were exposed to 25, 50 and 75% of LC50, respectively for four days. Blood samples for biochemical analysis (blood glucose, cholesterol and total protein) and gills for histology were taken after 96 hrs. After acute exposure to pesticide, significant changes were observed in serum bioch...

Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...re exposed to sub-lethal concentration (1/3rd of LC50) of DM+END mixture for 14-day. CAT was partially purified by using ammonium sulphate precipitation technique and kinetic characterization was also performed against different pH and temperature. CAT activity was measured spectrophotometrically at A240 nm. Results showed that highest CAT activity was noted from the crude extract of control fish gills (140.66±0.71UmL-1) as compared to DM+END exposed fi...

Raza Ullah Khan*, Ahmad Khan, Mohammad Zameer Khan, Fayyaz Hussain and Sonia Saba 

... selected micronutrients concentration in shoot and root were determined once after harvest of crop and shown as mean of three replications. Results showed that different combination of P yielded significant response in plant growth parameter. It was found that split application of P equally as soil and foliar caused an increase in biomass and grain yield. With respect to post harvest P and K concentration in plant parts, al...

Amar Razzaq1, Abdur Rehman4*, Abdul Hassan Qureshi2, Iqbal Javed3, Raheel Saqib5 and Muhammad Nadeem Iqbal6 

...sed on relatively higher concentration of HEI infrastructures installed on the farms. Sprinkler irrigation system was mainly installed on wheat crop while the drip irrigation systems were installed on mango orchards. Therefore, one half of the sample consisted of modern and conventional farmers growing wheat crop and the other half of the sample consisted of modern and conventional farmers growing mango orchards. Economic analysis measures of benefit-cost rati...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...consequences at elevated concentrations. The bacterial cell utilizes a battery of copper detoxification and exclusion mechanisms of which multicopper oxidase (CueO) is an integral component. In addition to acting as copper regulatory elements, CueOs have been shown to possess laccase activity. In this study, we have cloned and over-expressed the CueO of locally isolated Klebsiella pneumoniae KW strain. The CueO protein was purified to homogeneity b...
Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...osine triphosphate (ATP) concentration, as well as nitric oxide synthase (NOS) activity and nitric oxide (NO) concentration catalyzed by NOS. Based on biochemical analysis of the above, results showed that O2- production could be induced significantly after cold stress at 15°C and heat stress above 30°C. SOD activity showed a similar changing profile as the concentration...

Ali Muhammad*, Yasser Durrani, Majid Suhail Hashmi, Ihsan Mabood Qazi, Muhammad Ayub and Saifullah 

Sabahat Noor*1, Shaukat Ali1, Hafeez-ur-Rahman1, Farhatullah2 and Ghulam Muhammad Ali1 

...different levels of salt concentrations separately. Four parameters i.e. sugar contents, proline contents, chlorophyll contents and relative water contents were recorded and compared in transformed and non-transformed plants. It was observed that under drought and salt stresses, proline contents, sugar contents, chlorophyll contents and relative water contents (RWC) were significantly higher in transgenic plants as compared to non-transgenic plants. From obtai...

Mohsin Iqbal1, Farid Asif Shaheen1*, Farah Naz2, Muhammad Usman Raja2, Muhammad Fiaz3 and Muhammad Nadeem1 

...treated with the highest concentration of B. bassiana as compared to that of M. anisopliae. These non-chemical control strategies will have significant contribution towards development of commercial formulations of bio-pesticides to be used against this grain beetle and other stored product insect pests. 

...

Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1 

...ading capacity at higher concentration of calcium chloride i.e. 0.5 M. Antimicrobial assay clearly indicated that pumpkin seed oil was very efficient against S. aureus, which is a gram positive bacteria. Pumpkin seed oil and control Streptomycin had almost same inhibitory zone that showed its effective use in food. In general, results showed that encapsulation is an effective approach for protection of active ingredients of pumpkin seed oil. 

...
Ghazunfar Rashid1, Muhammad Avais1, Syed Saleem Ahmad1, Muhammad Hassan Mushtaq2, Rais Ahmed3,*, Mahboob Ali1, Muhammad Naveed-ul-Haque4, Mehtab Ahmad5Mumtaz Ali Khan1 and Naimat Ullah Khan6
...i, Bajra and Sarson. The concentrations were lower in the afternoon in the leaves and in mature crops as compared to stem parts, immature plants, and in samples collected from plants during morning hours. The nitrate concentration was lower in samples collected from Ravi area, as compared to samples collected from villages of Pattoki and Okara. Blood samples were collected from animals feeding on above fodders; Spectrophotom...

Jafar Aqeel Naqi1, Abdul Mateen1, Dilawar Hussain2,*, Hafiz Muhammad Tahir2, Sajjad Hussain1 and Abuzar Tabasum1 

...n corpuscular hemoglobin concentration (MCHC). Average mean weight gain (11.03±0.8g) and average mean length (7.8±0.4cm) was observed maximum in T4 as compared to other dietary treatments. There observed a significant difference (P<0.05) in FCR among all the dietary treatments with highest in T4 (0.59±0.32). Dietary treatment T5 (10/500g) showed significance difference (P<0.05) in red blood cells (RBC...
Sajid Ali Khan1, Mazhar Hussain Ranjha1, Azhar Abbas Khan2,*, Muhammad Sagheer1, Amjad Abbas1 and Zeshan Hassan2
... solvent. Four different concentrations 5%, 10%, 15% and 20% were prepared by diluting in acetone. The data regarding mortality, growth regulation and repellency of T. granarium was observed. Repellency of plant extracts was tested using area preference method while for the growth regulatory potential of plant extracts, the adults insects were released in wheat grains treated with different concentrations of plant ext...

Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1 

...n were tested at various concentrations under in vitro conditions by poison food and disk diffusion techniques while best effective seven were trialed in glass house and field experiments to determine their efficacy against Xanthomonas oryzae pv. oryzae. In vitro and in vivo studies under different conditions showed significant response of Mentha piperita, Azadirachta indica and Aloe vera either tested individually or in combination of two or more than two dec...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro
...rane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination, pH was determined by digital pH meter. Motility was determined under microscope at10x magnification, morphology and live dead ratio was assessed using eosin nigrosin stain under 40x magnification,

 *Irum Javed1, Muhammad Asgher2, Sadia Noreen1, Humara Naz Majeed1 and Tanzila Sahar1

SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE
...that Yeast extract in 1% concentration was the best nitrogen source. Surfactants like Tween 80 and sodium dodecyl sulphat (SDS) showed no effect on the citric acid.

...

 Muhammad Akram and Faheem Aftab

THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
...plemented with different concentration (0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1,2,3,4 or 5uM) of TDZ for 24 days. Highest bud break (100%) was obtained at 0.005 uM TDZ after 14 days of initial culture. The same cultures were further maintained and subsequently obtained with 20.6 mm shoot length and 7.2 average number of leaves for another 10 days. Similarly, all other TDZ’s concentrations below 1 uM were also effective i...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
...tent. Minimum inhibitory concentration of heavy metals for all bacterial isolates was different. It was concluded that irrigated pathogenic bacteria are highly resistant to heavy metals.

...

 Tanzeela Riaz*†, Farah Rauf Shakoori, Syed Shahid Ali

Toxicity of phosphine against tolerant and susceptible populations of Trogoderma granarium collected from Punjab, Pakistan
... to different sub lethal concentrations of phosphine for 20 hours. Based on LC50 data, Gujranwala population was found to be most resistant to phosphine whereas Sargodha population was found the least resistant population. Gujranwala population required 113.5, 128.8 and 168.1% more phosphine concentration in 4

 Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4

Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan
...tudy period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment samples was 71.08±8.32 mg/L. Maximum Cr concentration 81.72±2.54 was recorded during February while minimum 61.24±1.23 mg/L during May. Among all the three fish speci...

 Zubair Ahmed#, Muhammad Farhanullah Khan, Habiballah Rana*

Toxicological effects of Haloxylon recurvum Bunge ex Boiss (Khar Boti) whole plant extract and novel insecticide chlorantraniliprole against maize weevil, Sitophilus zeamais Motschulsky
...antraniliprole. A serial concentration of extract i.e.,
1.2%, 2.4%, 3.6%, 4.8%, 6.0% and insecticide 0.00224%, 0.00448%, 0.00896%,
0.01792%, 0.02688%, respectively, were topically applied to adult S. zeamais.
Contact toxicity assayed by topical application showed that 3.6% concentration of
H. recurvum plant extract and 0.00896% concentration of chlorantranilipro...

 Anber Naqvi1*#, Abdul Qadir1, Adeel Mahmood2, Mehvish Mumtaz3, Iqra Aslam1, Gan Zhang4

Assessment of polychlorinated biphenyls (PCBs) in maternal blood serum from selected districts of Punjab, Pakistan
...akistan. The mean
concentration of Σ34 PCB congeners was recorded 232.3 ng/g l.w. PCB homologs
profile showed highest levels of Tetra-CBs (53%) followed by Penta-CBs (23%)
and Hexa-CBs(11%), respectively. Spatial distribution of PCB homolog reflected
the higher prevalence of lower chlorinated PCBs in urban population descending
towards rural population. The mean TEQ concentration of e...

 Siddra Tayyab Akhtar, Anjum Nasim Sabri

Twitching, swimming, swarming in biofilm forming strains in response to chemical and physical factors
...tilities when the
concentration of NaCl increased in medium. Among chemical factors, tobramycin
was proven to have excellent efficiency to reduce bacterial motilites whereas
proteinase K enzyme showed weak antimotility effect against all tested bacterial
strains. From this study we proposed that instead of biofilm detachment by
expensive commercial detergents, we could change the physical environment of
sewage systems ...

Muhammad Asghar1*, Mirza Muhammad Qadeer Baig1, Sanaullalh Chaudhary1 and Muhammad Anjum Ali2 

...noconazole) with various concentrations of sprayable formulations of macronutrients (NPK) was conducted during Kharif 2012 to 14 at Adaptive Research Farm, Gujranwala, Pakistan. The experiment was conducted on a promising basmati rice cultivars Super basmati in RCBD with three repeats. The spray of difenoconazole at rate of 315 mlha-1 + 500gha-1 of NPK (20:20:20) significantly decreased incidence of BLS per leaf (9.31) and resulted in significant increase in 1...
Atiq-ur-Rehman1,2,*, Abida Latif1,*, Rukhsana Anwar1, Nasir Abbas1, Sajid Nawaz2 and Hamid Turab Mirza3
...ibitory assay. Different concentrations of fractions (20-100 µg/ml) were subjected to in vitro α-amylase inhibitory assay. Assessment of anti-hyperglycemic potential of the most active chloroform fraction exhibiting in vitro α-amylase inhibition was carried out in Wistar albino rat models. Changes in postprandial levels of blood glucose in rat models (normal and diabetic) were examined after starch load with or without chlorofor...
Muhammad Muddassir Ali1,2 and ─░brahim Hakk─▒ Ci─čerci1,*
...mn;1) at 200 µg/ml concentration and 11.67±2.52 at 50 µg/ml. Ethanol extracts showed the 2nd highest DNA damage (19±2)at 200 µg/ml concentration and 11 ±1at 50 µg/ml. While least was observed in the hexane extract (10.33±1.15; 6±2) at both concentrations, respectively. All groups were significantly different (P<0.05) ...

Misbahullah, Shad Khan Khalil, Asim Muhammad*, Afza Tabassum and Shah Fahad  

...rghum extract with three concentrations (1:3, 1:4, 1:5), weedicide (affinity) with three different levels (1/3, 1/ 2, 2/3 of the recommended dose) and 3 times of application (emergence,tillering,50% at emergence + fifty percent at tillering). Herbicide sole, weeded by hand and un weeded plots used for comparison. Results showing that concentration of sorghum and appliance point considerably increased days to heading, anthesi...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
... lead (Pb) and zinc (Zn) concentrations in bat tissues through atomic absorption spectrophotometer. Our findings showed that metals were more concentrated in the liver and kidneys, Zn and Pb showed high mean values as compared to other metals but these values were below the lethal levels. Within the regions and sexes no significant variations were found in metal concentrations; however within three bat species metal
Muhammad Ijaz1, Shahid Hussain Farooqi1, Rahmatullah2, Amjad Islam Aqib1, Sadaqat Ali3,*, Awais Ghaffar1, Ahmad Ali1 and Sehrish Saleem1
...copper, iron and lithium concentrations significantly (p < 0.05) decreased while the potassium concentration significantly (p < 0.05) increased in diarrheic foals. Hence, this can be concluded that diarrhea in foals have significant correlation with PCV, serum electrolytes, and trace and ultra-trace elements.
...
Mirza Imran Shahzad1, Hina Ashraf2,*, Muhammad Arshad3, Sabeeha Parveen4, Amna Aslam4, Nargis Naz4, Zahid Kamran1, Sumbul Gohar Khalid5, Sajid Hameed1, Muhammad Ashfaq6 and Muhammad Mukhtar7

 

...urther used in different concentrations and IC50 of each extract was calculated. The extract of Achyranthes aspera was found most effective with IC50 3.125μg and the extract of Oxystelma esculentum, was found least effective with IC50 60μg in the series. The current study has established the fact that Cholistani plants are rich source of anti-NDV agents and can be used in purified or in crude form.
...
Fatih Korkmaz1, Veysel Parlak2, Özgür Kaynar3, Arzu Ucar2, Gonca Alak1,* and Muhammed Atamanalp2
...lts in increased protein concentration compared to other coatings and control group (p<0.05).
...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...growth hormone and IGF-1 concentrations and average body weight than those in the control group (P < 0.05). Immunisation with ptCS/2SS-asd promoted the growth of rams similar to the effect of the simple co-injection of CpG and SS DNA vaccine. Thus, the fusion type of CpG adjuvant is an effective, simple, and low-cost method in enhancing the effect of SS DNA vaccine and the growth of ram lambs.
...

Nighat Seema1*, Muhammad Hamayun1, Husan Ara1 and Raham Sheer Khan

...ght stress, ammonium ion concentration was increased in all endophytic treated soil except that of A. niger. Similarly, inoculation of endophytic fungi did also increased the value of soil nitrate ions in the range of 26.86±1.34~39.39±1.97 mg kg-1 with or without drought stress. Inoculation of endophytic fungi increased the amount of extractable P and K in growth media compared to soil samples before experiment and after experiment in control sam...
Asim Faraz1,*, Muhammad Younas2, Abdul Waheed1, Muhammad Yaqoob2 and Kashif Ishaq3

 

...a, Mg, Cu, Zn, Fe and Mn concentrations were found to be significantly different (P<0.05) between calf groups being higher in IMS as (685.07±15.86, 595.67±15.86; 104.33±5.12, 101.17±5.12; 7.08±0.34, 6.73±0.34; 65.33±2.25, 59.33±2.25; 322.20±8.67, 311.10±8.67; 46.53±1.83, 40.67±1.83 mg/dL in male and female) than SIMS (523.18±15.86, 486.98±15.86; 80.58±5....
Aqeel Mehboob, Syed Muhammad Zaka*, Muhammad Sarmadand Maryam Bajwa
...iculata at 125 mg/ml concentration was maximum (83.64±12.51), followed by P. nigrum and M. oleifera at 125 mg/ml concentration were 79.76±14.60 and 30.57±4.80, respectively. The higher oviposition deterrent index was recorded in M. paniculata extract (87.32±0.77) as compared to P. nigrum and M. oleifera extract i.e. 82.58±2.14 and 77.25±1...
Muhammad Huzaifa Mehmood1, Muhammad Ahmad Iqbal2, Muhammad Daood1, Muhammad Rizwan Tariq3*, Khubaib Ali3
...d lipid profile and PUFA concentration was significantly affected by the incorporation of oat in rabbit feed. It was concluded that the supplementation of 2% oat in rabbit feed increase n-3 PUFA in meat while improvement in serum lipid profile was observed by 4% supplementation of oat seeds. It was also observed that fat percentage in meat was also reduced by oat seed supplementation.
...
Rahat Afza, Muhammad Afzal, Muhammad Zeeshan Majeed and Muhammad Asam Riaz*
... the impact of sublethal concentrations of insecticides on intra-guild predation (IGP) and aphid consumption by coccinellids. Data revealed that in the absence of prey larvae of C. septempunctata exhibited more predation on C. transversalis while the predation of C. septempunctata on C. transversalis was relatively less in the presence of prey. The larvae of C. septempunctata were found stronger and competitive than larvae an...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
...ncorporation method. Two concentrations of a NPV based formulation (Somestar): 1x107 and 1x108 POB/ml, and three concentrations of a Bt based formulation (Dipel): viz., 0.1, 0.3 and 0.5 μg/g, were applied alone and in different combinations against 2nd and 4th instar larvae of S. litura. The highest mortality of larvae was recorded after 7 days of applicatio...
Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1
...e of Hemodialysis on the concentration of the CXCL8 in serum and the levels of CXCL8 mRNA in neutrophils were respectively measured by ELISA and PCR. The house keeping gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference, the ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in neutrophils of the chronic renal failure were (128.892±17.101) pg...
Peng Song1,2, Wei Feng2, Haiying Shi2,Jinsheng Zhao3, Renmin Liu3 and Wei Xu2,*
...e maximum decolorization concentration of the Amaranth reached 1450mg/L. Mg2+ and Fe3+ could accelerate Amaranth decolorization, and Cu2+, Zn2+, Co2+ showed inhibition of Amaranth decolorization. Compared with the traditional method dissolving oil-soluble dye in DMSO, HP-β-CD was used to monitor the decolorization rate of the sudan I and appropriate molar ratio of HP-β-CD to dye (nC:n<...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
...the minimal bactericidal concentration, change of pH in the first 12 h and OD values of biofilms. Besides, fluorescent images of exopolysaccharides and living bacteria of Streptococcus mutans were measured with confocal laser scanning microscopy (CLSM). In our study, capsaicin showed an outstanding performance in the inhibition of acid producing and biofilm of the four strains of cariogenic bacteria. Meanwhile, low values of MIC of capsaicin on four sec...

 Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2

...e treated with different concentrations i.e., 2, 4, 6 and 8 mg/ml of ethanolic extract and motility of the worms was examined after every two to eight hours. Both A. lebbeck and C. sinensis exhibited 88% and 95% mortality at 6 & 8 mg/ml after 8 hrs of treatment. In conclusion, the ethanolic extracts of A. lebbeck L. and C. sinensis L. showed in vitro anthelmintic activity against H. contortus at

Ali Awais, Charassri Nualsri and Watcharin Soonsuwon* 

...treated with varying EMS concentrations. With the increase in concentration, there was a continuous decrease in germination, shoot and root lengths respectively. Data have been subjected to regression analysis and effective doses of EMS were recorded for both genotypes. The effective value calculated for shoot and root length in Dawk Pa-yawm was 1.34% and 1.33% and in Dawk Kha 50, 1.23% and 1.86% respectively based on the re...

Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1

...through ELISA. Different concentrations of SA and BTH were exogenously applied to activate the inherent resistance of mungbean by the production of defense associated compounds. All treatments were supportive in suppressing plant infection as compared to infected control, but the most promising outcome of treatments was observed when SA and BTH were applied at the concentration of 5mM and 150mg/L respectively. Three weeks&rs...
Fariha Latif* and Muhammad Javed
...50 and lethal concentration. These acute toxicity tests were performed at constant temperature (30oC), pH (8) and total hardness (250 mgL-1). All the three fish species varied significantly in their sensitivity towards metals mixture with the C. catla beingsignificantly highly sensitive followed by L. rohita and C. mrigala. After the acute toxicity tests, fish were exposed to 2/3rd of their respe...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
...ancorpuscular hemoglobin concentration) quantity within both animal categories.
...
Hafiz Abdullah Akbar, Zafar Iqbal, Waqas Raza*, MuhammadUsman Ghazanfar and Salman Ahmad
...mbination with wax and 3 concentrations of salicylic acid alone and in combination with wax. The study revealed that in case of plant extracts maximum growth inhibition (35.90 %) was shown by Anar followed by Chabir (33.60%) and Garlic (29.16%), while minimum growth inhibition was recorded from Parthanium (17.43%) and Ginger (19.94 %). In case of plant extracts + wax maximum growth inhibition (44.36%) was shown by Anar+Wax followed by Chabir+Wax (42.78%) and F...
Song Jiang, Fa-Lin Zhou, Jian-Hua Huang, Qi-Bin Yang* and Li-Shi Yang
...ery 5 d. Bacillus concentrations in the Y1, Y2 and Y3 groups were 10 ppm, 15 ppm and 20 ppm, respectively, and the Lactobacillus concentrations in the R1, R2 and R3 groups were 20 ppm, 25 ppm and 30 ppm. The control (C) group had no microecological preparation added. Results showed that the Y2 larvae achieved the heaviest body weights, which were significantly higher than those of all other groups (P <...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...was recorded the optimal concentration for the maximal cellulase activity. SDS-PAGE analysis confirmed the molecular weight of recombinant protein as 39 kDa. The protein was found thermostable which retained more than 70% residual activity with an incubation of 1.67h at 90 °C in the presence of cobalt. Presence of ionic and non-ionic detergents showed an inhibitory effect on the enzyme activity. Kinetic studies of recombinant protein demonstrated the k<...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
...e susceptible to certain concentrations of Huwa-San TR50: 4,000, 8,000, 10,000, 12,000 and 14,000 ppm. A marked reduction in the movement of both tick species was observed 24h of applying Huwa-San TR50, at 10,000 ppm and above. This study recorded an increase in percentage mortality with increasing concentrations of Huwa-San TR50 and this resulted in mortalities of 66.5% for H. dromedarii and 76.5% for H. impeltatu...
Tahira Moeen Khan* and Amat ul Mateen
...s a linear curve between concentration and absorbance. The uv-vis spectrum revealed the shift of absorption towards longer wavelength with the increasing concentration of plant extract. XRD indicates CuO Nps particle size is in between 14-20 nm. CuO nanoparticle show very significant antibacterial activity against four bacterial strains three gram negative (Escherichia coli, Proteus mirabilis, Salmonella) and one gram...

 Syed Turab Raza1,*, Zhu Bo1,*, Zulfiqar Ali2 and Tang Jia Liang3

... cow manure in different concentrations. Vermicomposting through biochar (Biochar 50%, rapeseed residues 20% and Biochar with earthworms) gave maximum increase in soil fertility with maximum decrease (39.63%) in C:N ratio. Significant increase of N content in nutrients and reduction in C: N ratios during this process shows conversion of harmful wastes into useful fertilizer.

...
Shehzad Ghayyur1,3, Sadia Tabassum1, Munawar Saleem Ahmad2Naveed Akhtar1 and Muhammad Fiaz Khan1,*
...

 Effect of three concentrations (0.25, 0.75 and 1.25ppb) of chlorpyrifos administered for 96 h has been studied on hematological and seral biochemical components of Oreochromis mossambicus. RBCs, Hb and Hct decrease significantly (p < 0.05) indicating an anemic condition, while the WBCs and platelet counts increased significantly (p < 0.05) in all the treated groups compared to the control group. Among the seral biochemical parameters, the level ...

Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
... larvae. However, higher concentrations of A. indica oil can be used to control CLM larvae.

...

 Abdul Khaliq1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Akhlaq Ahmad2 and Yasir Iftikhar3

...esponse was noted at 10% concentrations of all plants but E. Camaldulensis (5%) was least effective against all insects after control. Resurgence response in F1 generation in each experiment showed high multiplication rate at low concentrations and vice-versa also suppressed against N. tabacum and C. procera. Hence, developing PH3 resistance can be managed with bio-pesticides up to ...

 Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2 and Muhammad Anjum Zia3

...i> exposed to sub-lethal concentration for 14 days was evaluated. After purification, specific activity of enzyme was 1750.13 and 1272.72 U mg-1 in kidney of metal exposed and control fish, respectively. The enzyme from metal exposed kidney was 2.68 fold purified with 58.73 % age recovery. Results of characterization showed that SOD had broad range of pH from 4 to 8.5 but show maximum activity at pH 7 and 7.5 for stressed and control fish, respectiv...

 ┼×aban Çelebi

... their combination on Se concentration in liver, heart, kidney and breast muscle. In addition, malondialdehyde (MDA) concentration and enzyme activities of glutathioneperoxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) in liver, heart, breast and thigh tissues of laying hens were determined in present study. A total of 96 White Lohman laying hens, aged 24 weeks, were randomly divided into 4 groups (n=24), each...
Fazal Abbas1, Muhammad Ijaz1,*, Zunaira Akhtar1, Khalid Mehmood2, Muhammad Zeeshan Hyder3 and Umair Iqbal4
...The sodium and potassium concentration in ppm significantly increased (P< 0.001) while the calcium, copper and iron concentration in ppm significantly decreased (P< 0.001) in the calves having diarrhea. Copper concentration is related to the packed cell volume as in anemia copper deficiency is a significant feature. It was concluded that neonatal calf diarrhea is much common in calve...
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
...ector. Effect of various concentration of Isopropyl β-D-1-thiogalactopyranoside (IPTG), post induction time, effect of temperature and pH were examined for the maximal production of recombinant cellulase. The effect of supplementation of LB medium with additional carbon and nitrogen sources was also analyzed for maximal production of recombinant protein. Higher level enzyme activity was recorded at 25°C, pH 7.0 when the cells were induced with 0.5 m
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...pectively when used at a concentration of 1mM, however, the enzyme was almost completely inhibited by Pb+2 and Hg+2. Purified polygalacturonase showed pectinolytic effect in juice clarification by attaining maximum clarity (95% transmittance) after incubation at 50°C for 60 min. Total amount of phenolic and free radical scavenging activity of juice was improved after clarification. To understand the effect of juice clarification param...
Bushra Siddique1,*, Muhammad Tariq1, Muhammad Naeem1 and Muhammad Ali2
...ilter paper strips at 3% concentration. The filter paper strips were placed in arms of olfactometer. The control arms were treated with n-hexane. Data pertaining to preference of C. septempunctata and D. rapae after treatment application were recorded and analysed statistically. It was found that T6 (β-pinene + E-β-Farnesene) exhibited highest mean number entries of C. septempunctata (6.13%) and highest m...
R─▒fat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
... the rising amount of TA concentration. In our study, in 25, 50, and 100 μM TA groups, cyclooxygenase-2 (COX-2) decreased incrementally compared to the control group. Considering overstimulation of COX-2 paths in emergence of cancer or in metastasis, this study shows TA has an antiproliferative and stimulating effects on apoptosis of LNCaP cells based on the dose and duration of exposure.
...

Hamid Ali, Muhammad Arshad, Ibad Ullah Jan, Muhammad Zamin*, Junaid Khan, Ikram Ullah and Mushtaq Ali 

...f tuberose under various concentrations of gibberellic acid and micronutrients. Experiment was laid in Randomized Complete Block Design (RCBD) using two factors i.e. gibberellic acid and micronutrients with three replications. Both the factors gibberellic acid (GA3) and micro nutrients had their significant effects. The best performance was found in the interaction of GA3 and micronutrients for plant height (53,21cm), number of leaves (16.34), length of spike ...

Muhammad Usman Ghani1*, Muti Ur Rehaman Khan1, Asim Aslam1, Zubair Shabbir2, Li Bo3 and Naveed Anwar4 

..., platelets count and Hb concentration in diseased animals representing hematological investigation.  

...
Sun Hong1, Zhang Yifeng1, Wang Baishi2, Li Yangwei1, Xu Wenbo1, Mao Runkun1 and Wang Zhenglong1,*
...dioxide (CO2) concentrations and the temperature and humidity in the underground burrows of mandarin voles in Xinzheng, Henan Province, China during the spring and summer of 2015. Our results show that 1) the depth and complexity of the burrow were higher during summer than during spring; 2) maximal CO2 levels (2.55%) and minimal O2 levels (16.04%) were recorded in the underground burrows during summer; and 3) the temperature a...

Aliya Ayaz, Gohar Ayub, Maqsood Khan* and Syed Aizaz Ali Shah 

...r A was gibberellic acid concentrations i.e. 0, 20, 40 and 60 mgL-1, while factor B was comprised of dipping durations i.e. 0, 6, 12, and 18 hours respectively. Gibberellic acid concentrations and dipping durations had significant effect on the growth and yield of bitter gourd. Gibberellic acid concentration at @ 60 mgL-1 significantly reduced days to emergence and days to first flower, wh...

Zahin Anjum1*, Farhat Shehzad1, Amina Rahat1, Hamid Ullah Shah2 and Saleem Khan3 

...d Dibenz(a,h) anthracene concentrations were also found high in fish and mutton. The observed values were greater than permissible limits determined by Codex Alimentaurius and World Health Organization. High concentration of Polyaromatic Hydrocarbons was observed in coal grilled meats. Anthracene content was found high in mutton meat and mean value was 12.50 µg/kg-1; flouernthene was assessed high in fish meat with mea...
Iqra Jaffar, Zainab Sehzadi, Muhammad Adeel, Khalid P. Lone and Mehwish Faheem*
...iles were exposed to low concentrations (10μg/l and 100μg/l) of di-n-butyl phthalate (DBP) for 7 days to evaluate oxidative stress in liver and gills by analyzing activity of glutathione-S-transferase (GST), catalase (CAT), glutathione (GSH) and by measuring lipid peroxidation (LPO). Catalase activity increased significantly in liver and gills of fish exposed to 10μg/l of DBP. Level of GSH and LPO increased significantly in gills while a non-significa...
Abid Hussain Shahzad1, Abdul Sattar1,*, Nasim Ahmad1, Ijaz Ahmad2, Deniz Nak3 and Yavuz Nak3
...7), respectively. The P4 concentration has significant (P<0.05) difference among OVP0 (6.52±0.32), OVP5 (7.75±0.38) and OVP7 (7.58±0.26) on d30 post AI. This difference was non-significant (P > 0.05) on d60 post AI in OVP0 (6.37±0.49), OVP5 (6.75±0.36) and OVP7 (6.80±0.41), respectively. On d30 post AI, pregnancy rate was 39.70, 42.60 and 45.50% in OVP0, OVP5 and OVP7 groups, respectively ...
Mohsan Iqbal1, Farid Asif Shaheen1, Rashid Mahmood2,*, Muhammad Khalid Rafique2, Imran Bodlah1, Farah Naz3 and Muhammad Usman Raja3
... observed in synergistic concentration of EPB as compared toEPF. Synergistic concentration (1×108) of bacteria gave the best results against C. chinensis as compared to that of entomopathogenic fungi. The maximum percent conidial germination (90%) of B. bassiana and percent mortality (50.40±2.20a), (79.70±0.28a) and (90.76±0.56a) of C. chinensis were observed ...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
... in Friesian bull. Sperm concentration and motility were significantly (P<0.05) high in Jersey bull while live sperm percentage was significantly (P<0.05) high in Jersey bulls and sperm abnormality was significantly (P<0.05) high in Sahiwal and Friesian-Sahiwal cross. In addition, semen quality was improved in all the breeds after supplementation of vitamin C. There was no significant effect of BCS on the sperm quality of bulls. The results give impor...

Imdad Ali Mahmood1, Muhammad Imran2, Muhammad Sarwar1, Matiullah Khan1, Muhammad Aqeel Sarwar2, Shoaib Ahmed1 and Shahid Riaz Malik

...maturity to determine Zn concentration in plant tissues and grains. A significant increase in pods per peduncle, seeds per pod, grain yield and total biomass of lentil was observed with Zn application even at lower rate (5 kg ha-1) under PGPR inoculation. Maximum 100-seed weight (2.56 g) was recorded with PGPR inoculation which was 21 % higher than that of without PGPR inoculation. Overall, a significant increase (31 %) in grain yield and total biomass was obs...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...ificantly improved under concentrations of Vitamin C (400 mg/L) and Vitamin E (600 mg/L) (P < 0.05). Moreover, physiological indices of donkey semen were significantly higher than the control groups (P < 0.05) when Vitamin C (200 mg/L) and Vitamin E (200 mg/L) were present simultaneitily in the medium. Overall, the results suggested both antioxidants (Vitamin C and Vitamin E) could increase frozen semen quality and the ability of antioxidan...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
...was treated with various concentrations (5, 40, 80 and 100µM) of salicylidene acylhydrazide and 5-15 µM of proteases (proteinase K, trypsin, and chymotrypsin). Concentration dependent decrease was observed after 72 hours in biofilm formation of D. vulgaris on treatment with salicylidene acylhydrazide. Whereas 15 µM concentration of proteases produced significantly ...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
... showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48 h) was more active, being able to kill 100% of the Acanthamoeba cysts.The results showed the cysticidal activity of T. ammi essential oil, LC50 values estimated to be 73.31, 61.69, 57.21 and 53.01 μg/mL with 24, 48, 72 and 96 h, respectively. This is the first report of in vitro cysticidal activity of T. ammi essential oi...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...d along with the extract concentration and exposure time. Botanical extracts of Azadirachta indica (neem) and Gardenia jasminoides (gardenia) were the most effective with minimum LC50 (20.00 and 42.19%, respectively) and LT50 (47.97 and 71.26 h, respectively) values followed by Nerium indicum (oleander). Moreover, the essential oils of Datura alba (dhatura) and Syzygium aromaticum (clove bud) were the mo...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...xposed to the sub-lethal concentration (1/3rd of LC50) for 14 days. The CAT was partially purified by using ammonium sulphate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT remain lowe...

Sadaf Rahim and Mudassar Iqbal* 

... respectively at highest concentration after 2 hours exposure. Increased potency of extracts was observed with the increased exposure time. The LC50 value calculated for organic extract obtained from T. harzianum grown on PDB against Diuraphis noxia was 398.69 μg mL-1 after 3 hours with increased potency (237.46 μg mL-1) after 24 hours, whereas against Tribolium castaneum it was 213.85 μgmL-1 after 24 hours while its decreased potential was recorded (...

Muhammad Shafique, Nosheen Noor Elahi*, Muhammad Rashid, Amjad Farooq and Kausar Hussain Shah

...reased at 100-300mM NaCI concentration. Inoculation effectively increased the dry weights of plants at salinity levels of 0-50 mM NaCI as compared to uninoculated plants but was not effective at 200-300mM NaCI. Number and fresh weight of pods were not affected at 0-100 mM NaCI levels of salinity but strongly decreased at 200-300 mM NaCl. Number and fresh weights of nodules increased slightly at lower levels (20- I00 mM) of salinity but strongly decreased at 20...

Munazza Yousra1*, Sair Sarwar1, Muhammad Mahmood Ul Hassan1, Muhammad Zameer khan1, Abdul khaliq2, Shahbaz Ahmad1 and Shamim Ul Sibtain Shah3 

...n sources showed that Zn concentration was maximum on the first day in all treatments except control. Zinc release from Zn-EDTA was much higher as compared to ZnSO4. Similarly, the pot experiment conducted with the same set of treatments and crop responded well to Zn fertilizer application. The results showed a consistent increase in Zn concentration with the increasing Zn doses in both varieties. The maximum Zn

Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Gul Bahar Khaskheli2, Allah Jurio Khaskheli3, Arshad Ali Khaskheli4, Abdul Samad Magsi5, Ghulam Shabir Barham2, Arab Khan Lund5 and Maqbool Ahmed Jamali4 

...essed significantly high concentration of crude protein. Further the Prosopis juliflora against Zea mays, in Acacia nilotica versus Alhagi maurorum, in Tamarix orientalis versus Trifolium alexandrinum, Tamarix gallica versus Cordia sinensis (Linn.) crude protein content existed statistically non-significant, but each of above set varied significantly to one another. Trifolium alexandrinum and Zea mays though had statistically similar c...
Khansa Nazir1,*, Tariq Mukhtar1 and Humayun Javed2
...eniles was recorded at a concentration of 100 mg/ml followed by 75 mg/ml of AgNP. The minimum mortality was recorded with 25 mg/ml of AgNP. With the increase in concentration, there was a corresponding increase in the mortality of juveniles showing a direct relationship between mortality and concentration of nanoparticles. The effect of time on mortality was also found significant. With th...
Riyadh S. Aljumaah1, Mutassim M. Abdelrahman1,*, Moez Ayadi1 and Abdullah H. Alyemni2
...he groups, however, urea concentration was significantly (P<0.05) in the T1 and T2 groups. In conclusion, feeding Najdi ewes a diet containing higher energy and protein than recommended by National Research Council (1985), in comparison with the traditional feeding system, improved the lambing rate and growth of the newborns.
...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...s of the frozen mass and concentration of enzymes was determined. It was found that more than ~85% mass of enzymes, migrated along with freezing front and concentrated in the centre in case of radial freezing. To examine the effect of unidirectional freezing over extracted enzymes, the working activity of separated enzymes was checked by performing desizing test for alpha-amylase and biopolishing test for cellulase. The noteworthy results of desizing and biopo...
Naimat Ullah1, Sarzamin Khan2, Muhammad Mobashar3, Shahbaz Javaid4*, Tarique Tonio1 and Asif Mahmood5
...perm motility (%), sperm concentration (million/ml), live to dead ratio of sperm, amplitude of lateral head displacement of sperm (µm) and straightness of sperm (%) were measured at interval of 15 days. The results showed that dietary supplementation of fat didn’t influence dry matter and water intakes as well as weight gain significantly (P>0.05) among different treatments. Body conditions score was recorded significantly higher (P<0.05) in ...
Ali Mujtab Shah1,2,3, Muhammad Naeem2, Muhammad Giasuddin Shah2, Muhammad Haaroon2, Quanhui Peng1 and Zhisheng Wang1,*
...rom the present study theconcentration of serum IgG following administration of colostrum by ST method increases at a rate to that in calves fed by NS and NB and reaches a level consistent with high calf survival, it is also concluded from the study that the body weight gain is increased by ST feeding. 
...

Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3

...luding different glucose concentrations, nitrogen sources, pH, temperature and incubation period influence on biomass and different enzymes viz; xylanase, CMCase and laccase production by Pleurotus ostreatus were optimized in shake flask cultures. The results indicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (...
Xiaopeng Tang1, Wen-qin Su1 and Re-jun Fang1,2,*
...orphology and phosphorus concentration in the cell supernatant were measured after 24 h of CT treatment. The NaPi-IIb protein expression was determined by Western Blot, and the NaPi-IIb mRNA expression was determined by RT-PCR. The results showed that, compared with the control group, different levels of CT had no effect on cell proliferation, but it inhibited (P < 0.05) the absorption of phosphorus at CT concentration...
Constance Obiageli Ejilibe1, Helen O. Nwamba2, Ifeanyi Chinedu Atama3,*, Chiamaka Lynda Ani2, Ifeanyi Oscar Aguzie3, Josephine C. Madu3 and Christopher Didigwu Nwani3
...) and total protein (TP) concentrations in homogenized muscle samples was determined by standard procedures. ALT, AST and ALP increased significantly in response to concentrations of Butaforce® (7.00, 9.00 and 11.00 µgL-1) and Termex® (15.00, 20.00 and 25.00 µgL-1) used. The TP decreased in response to the same concentrations ...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5 
...upplemented with various concentrations of L-cysteine 0 mM, 0.5 mM, 1.0 mM and 1.5 mM. Cooling and equilibration of the diluted semen was done at 4°C for 4 h subsequently filled in straws at the same temperature and were cooled in liquid nitrogen vapors for 10 min. followed by storage at -196°C for assessment. Progressive motility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-cysteine supplemented groups in comparison wi...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
... mg/kg), decreased sperm concentration (for 110 mg/kg dosage), decreased motility (for 110 mg/kg dosage) and increased sperm malformation (for all of the treated groups) were also confirmed (p<0.05). Sperm malformation showed high sensitivity to PFOS exposure. A sperm malformation percentage, varying from 11.00% to 51.20%, was confirmed for the treated groups. As to various sperm malformation types, curved body type constituted the most proportion. T...

Chukwuemeka Calistus Okolo1*, Ikenna Onyema Ezeh2, Chinelo Nnenna Uju3 and Nwakaego Ernestina Nweze1 

...te count and haemoglobin concentration compared to groups A--C, and E, no significant variation was seen in the total and differential leucocyte counts across all infected groups. By day 23 PS, groups A and B had similar serum alanine and aspartate aminotransferases, blood urea nitrogen, and serum creatinine levels as group D. The serum malondialdehyde and catalase levels were similar in groups A--D. Therefore, treatment with the probiotics, while enhancing cl...
Adel E.M. Mahmoud1, AdamCie┼Ťlak2, Paulina Szulc2, Anna ┼üukomska3 and Ma┼égorzata Szumacher-Strabel2,*
...d in higher (P<0.001) concentration of Holotrichs. The ratio of acetate to propionate increased linearly with inclusion of dried sugar beet in the diet, however statistically significant differences were found only in D4. On basis of presented data, it could be concluded that yellow corn could be partially of fully substituted by dried sugar beet pulp without negative impact on rumen parameters including dry matter digestibility.
...
Tanzeela Riaz1, Farah Rauf Shakoori2,*, Hareem Mansoor1, Sana Khan1 and Mushtaq A. Saleem1
...to figure out the lethal concentration (LC50) of emamectin, abamectin and spinosad alone and in various conbinations such as abamectin:emamectin (1:1, 2:1, 3:1, 1:2 and 1:3) and abamectin:spinosad (1:1, 2:1, 3:1, 1:2 and 1:3) against two larval instars (4th and 6th) of MBDIN and Lahore populations of T. granarium. The LC50 of abamectin, emamectin and spinosad for 4th larval instars of Lahore populat...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
...he effects of sub-lethal concentrations of manganese on antioxidant activity in the fish, Labeo rohita. Fish were acclimatized for two weeks in the cemented tanks prior to the start of experiment. Four groups (n=10) of one year old fingerlings of Labeo rohita were exposed to 96-hr LC50 and sub-lethal concentrations (2/3rd,1/4th and 1/5th of LC50) of mangane...

Matiullah Khan*, Shameemul Sibtain Shah, Atiqullah Khan, Muhammad Sarwar and Shoaib Ahmed 

...se in postharvest soil P concentration was recorded. Seed inoculation with PSB significantly increased grain and dry matter yield. It can be concluded from the study that good quality of SSP can be manually prepared from HRP which may increase the yield comparable to DAP. 

...

Masood Ahmad* and Abdur Rab 

...eraction between calcium concentrations and application time revealed that foliar application of 200 mM calcium at 5th leaf stage resulted in significant increase in leaf area, however application of the same concentration of calcium at 7th leaf stage resulted in maximum size and weight of daughter corm. It is concluded that foliar application of 200 mM calcium at 7th leaf stage increased the size and weight of daughter corm...

Muhammad Zeeshan Manzoor1*, Ghulam Sarwar1, Mukkram Ali Tahir1, Noor-Us-Sabah1, Ayesha Zafar1 and Sher Muhammad2 

...water increased the salt concentration of soil. Resultantly, the growth of plants and yield becomes at threat. The salt stress is translated into decrease in growth rate and resultantly, biomass production decreases significantly. This reduction is gradual, depending upon increase in salt concentration in the root medium, while each species has a critical threshold value where it was started showing negative response. Leachi...
Muhammad Suleman1,*, Abu ul Hassan Faiz2, Muhammad Shahbaz2 and Ayesha Riaz3
...s niger at different concentrations of wheat bran as carbon source (2.5, 3.0, and 3.5 %) on the production of xylanase. Submerged fermentation was carried out in 500 ml Erlenmeyer flasks using vogel’s medium at 35°C for five days. UV irradiated A. niger has significantly increased the xylanase production as compared to parent strains. Twenty minutes exposure of A. niger was considered suitable for the production of xylanase. Wheat b...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...d growth. Changes in GHR concentration affect signalling pathways, binding capacity and alters activity of GH. Therefore, the GH and GHR genes are considered important candidate genes. DNA samples of Marecha camel were collected from the Camel Breeding and Research Station at Rakhmani Bhakar, Pakistan. Two significant polymorphic sites were identified in GH gene and three in GHR gene. Out of these, T1720A polymorphism in GH and A211927G polymorphism in GHR gen...
Abdul Hanan1, Talha Nazir1, Abdul Basit1, Shahbaz Ahmad2 and Dewen Qiu1,*
...ted with three different concentrations (1×106, 1×107 and 1×108 conidia/ml), filtrate bioassay was estimated by applying 7th day fungal filtrate and binary combination (1ml conidia +1ml filtrate) of V2×V2, V3×V3 and V5×V5 were tested against M. persicae. Mortality data were recorded after 2, 4, 6 and 8 days post-treatment. In conidial bioassay, V-3 showed maximum aphid morta...
Muhammad Zameer Khan1, Sair Sarwar1*, Ahmad Khan1, Razaullah Khan1, Munazza Yousra1 and Muhammad Ilyas2
...ield components, N, P, K concentration and uptake by wheat. Two extractants namely, AB-DTPAand NH4OAc were tested for K extraction from soils. The results showed a high positive correlation between AB-DTPA and NH4OAc extractable K, plant growth and yield parameters. The amounts of soil K had positive correlation with shoot K concentration and grain yield of wheat with r = 0.48 and 0.49, respectively for...
Aqsa Pervaiz1*, Rukhshanda Afridi1, Zahra Pervaiz1, Rabia Masood1 and Hira Pervaiz2
...ere exposed to sublethal concentrations of methylmercury 0.044 mg/L, 0.055 mg/ L and 0.073 mg/L in tap water respectively. The fish exhibited tail and fin disruption, bulging of eyes, scales erosion, mucous secretion, hemorrhage, color change, slow down swimming, lake of balance, restlessness, swimming side-wise, retardation of opercular movement and quick sudden movements. The fish was anesthetized and liver were removed after 48 and 96 hours for histopatholo...
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
...ibited the growth at 1ml concentration, Local honey at 3ml concentration, Marhaba’s honey at 4ml concentration, and Young’s honey inhibited the growth at the concentration of 5ml.
...
Naveed Ahmed1*, Abdul Waheed1, Farrukh Siyar Hamid1, Imtiaz Ahmed1, Muhammad Abbas Khan1, Sohail Aslam1, Muhammad Adil Younis2, Seemab Ali1, Nadia Khan1 and Madiha Bashir1
... (Asha and Red) and four concentrations of Gibberellic acid (GA3) (0, 20, 40 and 60 ppm). Experiment was laid out on Randomized Complete Block Design (RCBD) with three replications. Data showed a significant difference among most of the growth parameters. Maximum head weight (2306.7 g), stem length (42.66 cm), number of leaves plant-1(15.73cm), root length (27.53 cm), yield (55.63 t/ha) was recorded for cultivar cabbage red with the appli...
Muhammad Aslam Farooqi*, Bakhtawar Irsa, Sajjad Ali, Asif Sajjad, Muhammad Waqar Hassan and Sohail Akhtar

 

...;5% R.H). Four different concentrations (i.e. 50 ppm, 100, 200 and 400 ppm) of each insecticide were evaluated against A. mellifera as derived from their recommended field doses. Mortality of A. mellifera was recorded at 0.5, 3, 6 and 24 hours after exposure to insecticides. To evaluate the toxic impact, median lethal concentration (LC50) of each tested insecticide was determined. The results ...
Muhammad Usman Akhtar1,2, Abdul Qayum3, Anshan Shan1,*, Shuli Chou1, Hyeonsoo Jo4, Syed Waqas Ali Shah4 and Ishfaq Muhammad5
...of different dietary RDP concentrations on nutrient intake, nutrient digestibility, nitrogen balance, blood urea nitrogen (BUN) and milk yield of lactating Beetal goats. Thirty five lactating Beetal goats were randomly divided into five groups (seven goats per group) in a randomized complete block design. Five isocaloric and isonitrogenous diets were formulated to contain 30, 40, 50, 60 and 70% RDP of dietary protein represented as 30RDP, 40RDP, 50RDP, 60RDP a...
Shad Mahfuz1,2, Shuyuan Wang1, Mo Chen1, Fei Zao1, Dong Zhen1
Zhongjun Liu4 and Hui Song1,3*
...tibiotic fed groups. The concentrations of serum immunoglobulin M (IgM) were slightly higher in FVW fed groups compared with the other groups. Serum albumen concentration was increased (p<0.05) in 4%FVW fed group than control and antibiotics fed groups. Our study demonstrated that dietary inclusion of Flammulina velutipes mushroomstem waste could be used at 4%level as a unique feed supplement to improve egg ...

Muhammad Abu Bakar1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1, Rashid Mahmood2, Ziyad Abdul Qadir2, Muhammad Arshad1 and Mubasshir Sohail3 

...d Neem oil) in different concentrations against percent mortality of Varroa mites. The essential oils were applied at different concentrations (100 ppm, 200 ppm and 400 ppm) and percent mortality data were recorded after 12, 24, 48 and 72 hrs of the application. The results of present study were showed that lemon oil and eucalyptus oils were best compounds to manage Varroa mites at 200 ppm concentrat...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*

 

... and malonaldehyde (MDA) concentration were detected by a total superoxide dismutase test kit and a malondialdehyde test kit. Genomic DNA and RNA were extracted from cells; Methylation was inhibited by treating HL7702 cells with 5-aza-2’-deoxycytidine (AZA), and the level was analyzed via the methylation-specific PCR (MSP) methods. The increases in the methylation rate of CpG islands in the CYP1A1 promoter region and expression of DNA methyltransferases ...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...e further clarified. The concentration of the CXCL8 in serum and the level of CXCL8 mRNA in PBMCs of forty-eight children were dynamically measured by ELISA and PCR. The ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in PBMCs in MPP children were (298.917±51.860) pg/mL and (1.848±0.525) lgcDNA/lgGAPDH. There were significant differences between the trial groups and no...
Muhammad Izhar Shafi* and Muhammad Sharif
...s the lowest available P concentration of 8.09 and 7.82 mg kg-1 was recorded in soils where SSP and NP were applied, respectively, without any amendment of HA. Weekly turnover and mineralization potential increased with incubation periods as well as with the application of different HA levels. Maximum weekly P turnover rate and mineralization potential were 1.35 mg kg-1 and 67.71 kg P ha-1 season-1, were recorded in ...

Asma Hassan*, Zuhair Hasnain, Shahzada Sohail Ijaz, Muhmmad Ansar, Muhmmad Rasheed, Ajaz Ahmad, Lubna Ayub Durani and Muhmmad Bilal Ali 

...the highest nitrogen (N) concentration was under MT with GW (5.5 mg kg-1), 2011-12 it was more in GW (6.13 mg kg-1) under MP. In winter from second year it was highest in MP with MC (8.4 mg kg-1). Phosphorus (P) and potassium (K) concentration second year it was highest under MT with MW (7.1 mg kg-1) and with MC (72 mg kg-1), respectively. The highest CEC at the end of winter from 2010-11 under MT in GW (3.67). Second year, ...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...treatment. Median lethal concentration (LC50) and median lethal time (LT50) values were calculated for each treatment using log-dose probit analysis. Results revealed a mortality response of termites directly proportional to the extract concentration for all treatments. The floral extracts of basil (O. basilicum) and Tecoma (T. stans) exhibited maximum termite mortality (i.e. 55.5 and 50.0%, respectively) with minimum LC50 a...

Muhammad Abdul Qayyum1*, Farhat Bashir2, Muhammad Mudassar Maqbool3, Anser Ali3, Saqib Bashir1 and Qaiser Abbas4 

...n because it has highest concentration of soluble salts. This study aims to examine the effect of NaCl salt at 100 mM and 200 mM concentrations on linseed (Linum usitatissimum L.) germination, plant growth, nutrients and soluble salts uptake. The results revealed that tested four linseed genotypes showed an overall germination rate of 86-94% at 100 mM NaCl (T2) and 78-84% at 200 mM NaCl (T3) and seedling survival rate of 80-...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...persons (15.2). Abnormal concentration of reactive oxygen species can disturb the body’s defense mechanism which cause potential oxidative injury to tissues and lead to cartilage degradation in RA patients.
...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...e, 5.5 pH, 5 mL inoculum concentration, 1.5 mL banana peel as substrate and 20 days incubation time. The antimicrobial activity of secondary metabolites was tested against Streptococcus aglactaie, Streptococcus pyogene, Escherichia coli and Proteus mirabilis. The fungus Aspergillus niger was found best among all tested strains as it produced maximum amount of antimicrobial agent Tautomycetin. The antimicrobial activities were determ...
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
..., pH 6.0 at 1% substrate concentration while MBL-GN02 produced maximum enzyme at 35oC, pH 4.0 with 0.5% substrate concentration. The MBL-SP02 produced maximum enzyme at 45oC, pH 7.0 with 2.0% substrate concentration. Among various carbon sources tested guar gum was the best producer of enzyme. Under these optimized conditions for each isolate, the best producers MBL-S...
Mehwish Faheem1*, Maleeha Rafeeq1, Aisha Majeed1 and Saba Khaliq2
...of acute exposure of low concentrations of BPA (10, 100, 1000 µg/l) on liver cyp1a1 and heat shock proteins mRNA level was evaluated using real time qPCR. Results revealed that exposure to bisphenol A caused decrease expression of cyp1a1 in a concentration dependent manner. Exposure to graded concentrations of bisphenol-A resulted in significant increase in mRNA express...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...the Group B, C, and D in concentration higher than 0.05 ppb. The AFB1 was excreted in milk as metabolite AFM1 @ 1.35-1.59%. Udder health and milk quality deteriorated as SCC and TVC increased. Levels of serum enzymes AST and ALT increased with ingestion of dietary AFB1. It is concluded that ingestion of very low level of AFB1 by lactating goats results in excretion of carcinogenic metabolite AFM1 in milk beyond the permissible level. Dietary AFB1 has role in s...
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
...step, minimum inhibitory concentration of test compounds was determined against bacterial species by using resazurin microplate assay. All the tested compounds exhibited variable antimicrobial properties against various test cultures. All the compounds showed stronger antimicrobial activity against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Compound III was found to be the most effective compound. These results demonstrate t...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...ed orally with different concentrations of levofloxacin (0.00, 9.37, 18.75 and 37.50µg/g BW of mice) for 30 days consecutively. Mice were sacrificed on 31st day (24 hours after last dose administered) under deep chloroform anaesthesia. Blood was extracted through cardiac puncture and viscera were dissected out for further analysis. Levofloxacin induced reduction in body weight while caused a noticeable increase in liver, kidney and testis weig...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...group (AD). A sub lethal concentration containing 15ug/g B.W of BP was given with and without turmeric orally, dissolved in corn oil, used as vehicle too. Dissections were made on 31st day, twenty-four hours after the last dose administration. Results showed that continuous administration of butyl paraben (BP) had decreased the organs weight as compared to control. Regarding biochemical parameters results had shown an increased enzymatic level of al...
Sumaira Abbas1, Muhammad Sultan Haider2,*, Fatima Kafayet1, Sana Ashraf2, Atifa Masood3 and Moazma Batool4
...re (1:1 v/v). Carotenoid concentration was determined by thin layer chromatography (TLC) and found satisfactory. By mixing fish meal, sunflower meal and rice polish four treatment diets, T1, T2, T3, were prepared and citrus peel powder was added @ 200g, 400g and 600g/ 1000g, respectively. While fourth one, control diet (To) was without citrus peels. These is nitrogenous diets (30% protein) were offered to Carassiu...

Hafiz Abdullah Shakir1*, Javed Iqbal Qazi2, Abdul Shakoor Chaudhry2, Muhammad Irfan3, Muhammad Khan1, Shaukat Ali4 and Saima Shahzad Mirza5 

...t; B > A. The highest concentrations (µg/g dry weight) of Cd (0.29), Cr (4.64), Cu (8.85), Fe (65.66), Mn (5.14), Hg (2.91), Ni (3.18), Pb (5.14) and Zn (72.16) were recorded at site C. Among the sampled fish species, C. mrigala showed highest potential of metals bioaccumulation than L. rohita and C. catla. Scales of the fishes caught during low flow season showed significantly (P<0.001) higher concentrations of ...

Waseem Abbas1, Shakeel-ur-Rehman2, Abdul Rashid2, Muhammad Kamran3, Muhammad Atiq2 and Muhammad Ehetisham ul Haq3* 

...ca were evaluated at 3 % concentration against eggs hatchability and adult emergence of the whitefly in lab condition. Two consecutive sprays were applied to assess the relative impact of different plant extracts against adult whitefly population and the disease incidence of cotton leaf curl virus disease in filed conditions. Azadirachta indica was found most effective to inhibit the egg hatchability and adult emergence as compared to other plant extracts appl...

Hassnain1, Abdul Basit1*, Mehboob Alam1, Imran Ahmad1, Izhar Ullah1, Noor Alam2, Inayat Ullah3, Muhammad Areeb Khalid1, Muhammad Shair4 and Noor ul Ain1 

...) environment. Different concentrations of chitosan (0, 50, 100,150 mg L-1) and various water stress intervals (3, 6, 9 and 12 days) were given to tomato plants from 15 days after transplantation. Results showed that various application of chitosan and water stress interval had a significant (P< 0.01) effect on almost all studied parameter. Tomato plants treated with 6 days water stress interval had maximum average plant height (82.69 cm), average numb...
Awais Bin Shahid1, Moolchand Malhi1,*, Saeed Ahmed Soomro1, Muhammad Giasuddin Shah2, Nazeer Hussain Kalhoro3, Asmatullah Kaka4, Rajoo Mal1, Muhammad Awais Soomro1Saba Parveen Samo1 and Muhammad Nawaz Sanjrani5
...aled that the molar concentrations of propionate and the total short chain fatty acid (SCFA) increased (P < 0. 005) and the pH decreased (P < 0.05) in the ruminal fluid of SY goats compared to C. The morphometry showed that the length, width and the density of papillae in the atrium ruminis, ventral rumen and ventral blind sac of rumen increased (P < 0.05) w...
Khursheed Ahmed, Shoaib Freed*, Rana Fartab Shoukat and Kanwar Waqas Ahmad
... with insecticides. Five concentrations i.e., 1×106, 1×107, 1×108, 2×108 and 3×108 conidia/ml of each isolate of entomopathogenic fungi were used in combination with insecticides against 5th instar nymphs of O. hyalinipennis. The combined treatment of isolates of entomopathogenic fungi and synthetic insecticides caused higher mortality of O. hyalini...
Hazrat Ali1,2,*, Ezzat Khan1,* and Muhammad Jamal Nasir3
...s designed to assess the concentrations of Cr, Ni, Cd and Pb in water, sediments and different fish species of River Shah Alam, a tributary of River Kabul in Khyber Pakhtunkhwa, Pakistan. The different environmental samples were collected from the river, downstream the city of Peshawar, and analyzed for the selected heavy metals by atomic absorption spectrophotometry. Heavy metal accumulation was studied in muscles of six fish species collected from the river....
Ammara Blouch1, Ata ul Mohsin2, Muhammad Naeem2 and Rashid Mahmood1*
...d with increase in their concentration and decreased with increase in larval instar. Similarly LC50 values suggested Turex (Bt sub species kurstaki and aizawai) to be more toxic with less LC50 values as compared to Dipel (Bt sub species kurstaki). These results indicated that these bio pesticides if used at early insect stage can help to control this pest. 
...

Noorullah Khan1*, Farrukh Siyar Hamid1, Fayaz Ahmad1, Sabaz Ali Khan2, Imtiaz Ahmed1, Muhammad Abbas Khan1, Shamsul Islam1, Abdul Waheed1, Basharat Hussain Shah1 and Hussain Shah

...ives to find the optimal concentration of IBA for better rooting and survival rate of seedlings from kiwi cuttings. The experiment was laid out according to randomized complete block design with three replications and 20 four budded branch cuttings of “Hayward” kiwifruit cultivar. The cuttings were treated with different concentration of IBA (0, 1000, 2000, 3000, 4000, and 5000 ppm) for ten second and then plante...
Hu Liu1,2, Qifang Yu1,2, Xiaopeng Tang1,2, Chengkun Fang1,2, Sijia Chen1,2 and Rejun Fang1,2,*
...e, egg quality, selenium concentration in eggs and serum antioxidant capacity in laying hens. Seven-hundred and twenty 21-week-old healthy Roman laying hens with a similar laying rate were randomly divided into 5 groups with 6 replicates of 24 hens. The experiment was designed by 2 × 2 factorial arrangement with two sources of Se and two levels of Se. The hens in control group were fed a basal diet without adding exogenous selenium source, and the hens i...
Umer Iqbal1,* and Tariq Mukhtar2
...arbendazim (79.11%) at a concentration of 150 ppm. Copper oxychloride at a concentration of 50 ppm gave the minimum inhibition (12.50%). Concentrations also had significant inhibitory effects on the growth of the fungus. All the fungicides caused maximum inhibition of the growth of the fungus at a concentration of 150 ppm. With a decrease in the

Ghulam Murtaza1*, Ghulam Sarwar1, Noor-Us-Sabah1, Mukkram Ali Tahir1, Fakhar Mujeeb2, Sher Muhammad3, Muhammad Zeeshan Manzoor1 and Ayesha Zafar1  

...in the reduction of salt concentration in the root zone. Organic matter was used to mitigate the negative effects of saline water irrigation using sorghum as test crop during summer, 2018. In this experiment water of 3 different types was applied coupled with addition of organic matter at 2 different rates. The experiment comprised of 9 treatments having 4 replications; T1 = canal water, T2 = saline water having EC value 2 dS m-1, T3 = saline water having EC v...
Monica Paula Marin1, Elena Narcisa Pogurschi1*, Iuliana Marin2 and Carmen Georgeta Nicolae1
...y metals, increasing the concentration of unsaturated acids (oleic, linoleic) and immunoglobulins in colostrum, as well as reducing the diarrheal gastric disease of cattle. By adding zeolite in the cow ration during the precalving period has determined a significant increase in blood serum calcium during the last week of precalving period, as well as in the calving and postcalving periods, having as consequence fewer recorded milk fever cases. The results obta...
Yan-Guo Han, Yu-Qing Han, Jia-Yuan Wu, Jia Guo, Ri-Su Na, Yan Zeng, Guang-Xin E, Yong-Ju Zhao and Yong-Fu Huang*
... growth factor 1 (IGF-1) concentrations were detected by radioimmunoassay. The offspring lambs in T group showed significantly lower serum SS and higher PRL concentrations than those in the control group, and there were no difference between T group and the control group in terms of serum GH and IGF-1 concentrations. There was a significant negative correlation between offspring lambs seru...
R. Yasmeen1*, S. Shaheen1, B.N. Khan2, S.S. Bokhari1, U. Rafi1 and A.W. Qurashi1
... water samples and small concentrations were noticed in soil samples. The amounts of Zn and Cu in feed were reflected in fecal samples; however, higher amount of Pb and Cd were noticed in fecal samples compared to feed, water and soil. This showed inhalation of these metals from the air. Amount of metals at different depths showed unique pattern low to high and again low that was irrespective of site location. Overall there was more exposure to air poll...

Nosheen Noor Elahi1, Muhammad Shafique1, Muhammad Imtiaz2, Umer Farooq3* and Muhammad Rashid1 

...creased at 20-100mM NaCl concentrations but drastically decreased at higher levels. While in varieties CM44 and CM2000 a gradual decrease of biomass with increasing salinity levels were observed. Number of pods, flowers and fresh weight of pods were not affected at salinity levels of 0-50mM and decreased at 100 mM NaCI in all the four varieties. In varieties CM91 and CM2000 nodules were formed at all levels of salinity treatments, while in varieties CM44 and C...
Yun-Feng Liu, Yong-Mei Guan, Dan Chen, Jia-Le Chen, Chen Jin, Wei-Feng Zhu, Lu Wu and Li-Hua Chen*
...skin and brain, the peak concentration (Cmax) and the area under the concentration-time curve (AUC0→t) of brucine and strychnine in co-administrated with PRAE were decreased significantly (P<0.05), and the Cmax and AUC0→t of paeoniflorin in co-administrated with SSE increased significantly (P<0.05). These results showed that the co-administrated of SSE...
Elmas Uluta┼č1,*, Abdullah Eryavuz2, Aziz Bülbül2, Abdur Rahman3,*, ─░smail Küçükkurt4 and Cangir Uyarlar5
...ptin and malondialdehyde concentration while other parameters remained unaffected. No difference was observed in ruminal pH between the treatment groups. Ruminal ammonia and number of protozoa were decreased (p<0.05) with 700 ppm and 1000 ppm zinc supplementation. Rumen Zn concentration increased (p<0.05) in the goats fed with 1000 ppm zinc, whereas there was no difference in rumen Fe and Cu co...
Nihat Ye┼čilayer*
...between total carotenoid concentration (TCC) and flesh colour, determined by visual and colorimetric methods, of three fish species. Linear relationships were observed between CIELAB colour measurements (lightness-L*, redness-a*, yellowness-b*, hue-H°ab and chroma-Cab), DSM SalmoFan card scores and TCCs. The redness (a*) was mostly correlated with TCC of the fish fillets. The FRT group had the highest TCC (9,75 mg kg-1) compared to the ot...
Tian-Yi Zhao1, Zi-Qing Liu2, Shu-Fei Ma3, Bo-Yang1, Fan-Fan Guo1 and Ming-Hua Duan1*

 

...;B signaling pathway and concentrations of TNF-α, IL-6, and IL-1β were measured. The results demonstrated that biatractylolide can reduce apoptosis in hippocampal cells, prevent and improve the cognitive decline induced by Aβ, prevent the morphological changes in hippocampal nerve cells and can reduce the activation of NF-κB signal pathway in vivo. Taken together, biatractylolide appeared to be a useful agent for the treatment of Aβ-...
Ju Zhang1,2, Dong-Sheng Jia3, Shi-Hao Zhao1,2, Xiao-Liang Xie3, Hong Chen4 and Hai-Feng Li4,*

 

...ild-type N2 nematodes at concentration < 0.6 mg/ml, and 0.05 mg/ml of the HGQ significantly prolonged the lifespan and reduced the lipofuscin level in N2 nematodes. However, the HGQ failed to influence the pharyngeal pumping rate in N2 nematodes or to affect the lifespan of sir-2.1 mutant nematode VC199, indicating the anti-aging effect of HGQ is independent of calorie restriction pathway. Further experiments showed that the HGQ decreased ROS and MDA...

Stanley Uchenna Onwudike* and Vivian Chizoba Edoziem 

...As a result of different concentrations of inorganic phosphorus forms within these roots zones, appropriate agronomic management practices such as crop rotation, mixed cropping and shifting cultivation is recommended for sustainable phosphorus management in the studied soil. 

...

Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui

...to establish the optimal concentrations of plant growth regulators and sucrose for micropropagation of sugarcane. Sugarcane is cultivated through cane sets and therefore, multiplication of any new genotype through traditional practices requires interminable period of time. Eight sugarcane genotypes viz. NIA-2004, SPF-234, NIA-2012, BL4, AEC92-1208, Thatta-10, Gulabi-95 and NIA-1026-P7 were subjected to tissue culture using four different combinations of shoot ...
Ahsan Numan1,4, Khadija Irfan Khawaja2, Abdul Basit Qureshi3Muhammad Shahbaz Yousaf4, Imtiaz Rabbani4, Hafsa Zaneb5 and Habib Rehman4*
...8-1.51) and triglyceride concentrations (OR:1.13, CI: 1.02-1.33). In conclusion, the DSP was considerably higher in DMT2 patients and was significantly associated with age of patients, duration of DMT2, HbA1c, blood fasting sugar and serum triglyceride concentrations. Proper management of DSP needs better management of DMT2.
...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...lpha;, IL-1β, IL-6) concentrations, urinary volume, creatinine, and both serum and urinary electrolyte levels were measured. In addition, the apoptosis rate of white blood cells was analysed from plasma samples. Tissue samples from the brain, heart, aorta and kidneys were used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF g...
Babar Khan1,2 *, Nazir Javed1, Sajid Aleem Khan1, Nasir Ahmed Rajput1, Muhammad Atiq1, Abdul Jabbar1, Abdul Rehman3, Anam Moosa1 and Muhammad Amjad Ali1*
...ssei. Four different concentrations(50, 100, 200 and 500 IJs) of tested EPN speciewere used against insect’s larvae. Reproduction rate of S. kraussei was highest at concentration of 500 IJs as compared to 50, 100 and 200 IJs. Similarly, the effect of different temperatures was also studied to evaluate the efficacy of S. kraussei on insect larvae. At 25°C, S. kraussei showed significantly hi...
Sumaira Akram1, Sajid Aleem Khan1*, Nazir Javed1 and Saeed Ahmad2
...gnificantly at different concentration of chemicals, bio control agents and plant extracts. Among chemicals, cartap was most effective with lowest egg hatching and among biocontrol agents Paecilomyces lilacinus (Thom) Samson (PL) produced highest egg hatching while, Trichoderma harzianum Rifai (TH) caused lowest egg hatching. In plant extracts the highest egg hatching was recorded in clove at S concentration an...

Asmaa A. Darwish 

...cerides, MMP-2 and MMP-9 concentrations was depicted in the three diseased groups. On the contrary, the total cholesterol, HDL-cholesterol, LDL-cholesterol, minerals, electrolytes, trace elements and total antioxidants capacity concentrations significantly (P< 0.05) decreased in the three diseased groups. Both of MMP-2 and MMP-9 yielded a sensitivity and NPV as 100% in the sheep pneumonia, arthritis and enteritis and corr...
Godwin Ikechukwu Ngwu1*, Maria Ifeyinwa Ngwu2, Fabian Chukwuemenam Okafor1, Aruh Ottah Anaga3 and Christopher Didigwu Nwani1
...ol. The results indicate concentration dependent mortalities and significant differences (P<0.05) in the mean percentage mortality between the laboratory-reared and field-collected Aedes mosquitoes. The mortality rate due to the extract was found to be less in the field collected A. aegypti (LC50 = 2.81 ± 0.14; LCI = 2.55; UCI = 3.11) than the laboratory-reared ones (LC50=1.24±0.09; LCI = 1.07; UCI = 1.43) a...
Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Muhammad Ibrahim Khaskheli2, Gul Bahar Khaskheli3, Allah Jurio Khaskheli2 and Arshad Ali Khaskheli1
...> pertained considerable concentration of crude protein, Zea mays high ether extract, Calligonum polygonoides beared significantly high level of carobohydrate.
...
Meriem Msaad Guerfali1,*, Heitham Hamden1, Salma Fadhl1, Wafa Djobbi1, Lotfi Sillini1, Wafa Marzouki1 and Mohammed Ammar2
...nd spinosad at different concentrations. Histopathological effects of spinosad were also studied and showed that for doses up to LC50, annoying effects begin to appear in glial and neurosecretory cells. The LC50 for malathion was 75.61 ppm for the populations treated more than 6 times. Low levels of...
Seval Dernekbasi* and Emin Karatas
...had significantly higher concentrations of n-6 fatty acids, predominantly in the form of linoleic (LA, 18:2n-6c) and oleic acid (OA, 18:1n-9c), while n-3 fatty acids were present in significantly higher concentrations in the FO group. The fatty acid composition of rainbow trout fillet reflected the dietary lipid source. Fillet of fish fed by the FO diet had highest eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic aci...

Hamza Rehman*, Nida Ahmad Khan, Asad Ali, Warda Batool and Kanwal Razzaq 

...tested at four different concentrations (0.25, 0.5, 1 and 2ppm). Mortality of insects was documented after 24, 48 and 72 hours. Abbot’s formula was used to determine the corrected mortality. At 2ppm concentration thiamethoxam and imidacloprid provided 87.57% and 78.59% mean mortality of khapra larvae on treated filter paper after 72 hours respectively. On treated wheat, thiamethoxam provided 82.61% while imidacloprid g...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
...ion after slaughter. The concentrations of 4-methyloctanoic acid and 4-methylnonanoic acid in waist subcutaneous adipose tissue were detected using gas chromatography. Immunisation of oral and plasmid-injected KISS1 DNA vaccines induced strong antibody response, significantly suppressed testosterone secretion and reduced scrotal circumference and testis weight. It also significantly reduced the concentrations of 4-met...

Pushpa1, Nighat Seema Soomro1, Shahla Karim Baloch2, Mehmooda Buriro1, Aijaz Ahmed Soomro1, Muhammad Tahir Khan3, Qamar Uddin Jogi1*, Muhammad Nawaz Kandhro1 and Farheen Deeba Soomro1 

...nverse proportion to the concentration of allelopathic weed powder. Shortest shoots (14.46 cm) and roots (3.20 cm) were seen in variety TJ83 at the highest concentration of Avena fatua powder (75g kg-1 soil). The minimum shoot fresh weight (0.13 g), root fresh weight (0.10 g), shoot dry weight (0.04 g), and root dry weight (0.03 g) were also noticed in variety TJ83 under the highest treatment of Avena fatua powder (75g kg-1 ...

Rehman Ali Keerio1, Nighat Seema Soomro1, Aijaz Ahmed Soomro1, Mohammad Aquil Siddiqui2, Muhammad Tahir Khan2, Ghulam Shah Nizamani2*, Muhammad Nawaz Kandhro1, Maryum Siddiqui4, Hajra Khan3 and Farheen Deeba Soomro

...vely on applying highest concentration of Zn. While, seeds weight head-1, seeds index, seed yield, and oil content ascended by 76.6%, 37.2%, 81.4%, and 43.2%, receptively as compared to the control. It was concluded on the basis of these findings that the foliar application of Zn in 2.0% concentration can be employed to increase the sunflower yield and oil content. The study suggests that Zn can be employed for improving sun...

Ahmad Naeem Shahzad1, Muhammad Kamran Qureshi2, Samee Ullah1, Muhammad Latif3, Shakeel Ahmad1 and Syed Asad Hussain Bukhari1* 

... in cotton plants. Three concentrations of trehalose (0, 5 and 50 mM) were applied to plant foliage, subjected to varying levels (0, 11 and 17 dSm-1) of salinity. Salt stress disturbed the sodium and potassium concentrations in leaves and roots of plants. Different morphological traits of plants including number of leaves, leaf fresh weight, shoot fresh weight, root fresh weight, plant height, leaf dry weight and root dry we...

Tahira Moeen Khan*, Irum Riaz, Shahida Hameed and Bushra Khan 

...aking 25ppm/50ml initial concentration of NaAsO2. Best removal (90%) was achieved for Lemon juice microwave activated date seed husk LMDS (time= 30mins, agitation speed= 150 rpm, pH= 5, adsorbent dose= 0.1 g). While 85% removal efficiency was observed for Raw date seed husk RDS (time= 45mins, agitation speed=250 rpm, pH= 4 and adsorbent dose= 0.5 g). The adsorption isotherms (Freundlich and Langmuir) and adsorption dynamic kinetic studies were also conduct...
Hifzulrahman1, Muhammad Abdullah1*, Muhammad Uzair Akhtar2, Jalees Ahmed Bhatti3, Muhammad Saadullah1 and Muhammad Naveed-ul-Haque2
... treatment increased the concentration of milk fat and milk fat yield by 3.5 and 11.2%, respectively, compared with the Ctrl. However, the Ca-FA treatment did not change the plasma urea nitrogen, glucose, and triglyceride, compared with the Ctrl. Similarly, the reproductive indices were not different between Ca-FA treatment versus Ctrl. In conclusion, the Ca-FA are useful to increase milk production and milk fat yield without effecting the plasma metabolites a...
Renlong Lv1*, Ken-Ichi Horiguchi2, Shin-Ichi Tagawa3, Shoichi Ichihara4 and Norio Yoshida
...tal volatile fatty acids concentration between two experimental diets, and the palatability of jatropha meal showed to be bad. However, the blood parameter and rumen fermentation did not show any abnormal value after using jatropha meal of 5% and 10% of concentrate in sheep.
...
Saeed Fatima1, Khalid Javed Iqbal1, Usman Atique2,3*, Arshad Javid4, Noor Khan2, Sonia Iqbal2, Hamid Majeed5, Hamda Azmat2, Bakhat Yawar Ali Khan6, Irfan7, Muhammad Tausif Shahid1, Gulnaz Afzal1
...ed the pattern of metals concentration in fish in the order of Cd > As > Hg. However, R. rita, O. niloticus and C. idella showed higher accumulation of the metals that clearly alluded to species-linked metal bioaccumulation, which might be due to their unique feeding mood. Cadmium presence ranged between 0.04 – 1.12 ppm while As and Hg fluctuated between 0.01 – 0.04 and 0.001 - 0.18 ppm, respectively. Conversely, higher b...
Asim Faraz1*, Abdul Waheed1, Annamaria Passantino2, Ayman Balla Mustafa3, Nasir Ali Tauqir4, Naeem Ullah Khan5, Muhammad Shahid Nabeel6
...level of Hb (hemoglobin) concentration (P<0.05) was found to be significantly different as 16.4±0.14 and 16.8±0.09 (g/dL) with G1 and G2, respectively. The concentration levels of cholesterol, triglycerides, urea, creatinine, albumin, total-protein, glucose, Ca and P were found significantly (P<0.05) varied among groups. Regarding hair mineral profile, concentrations of...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
...fat, lactose and mineral concentration are influenced by exogenous OT administration. It affects the cell maintenance and mammary metabolism along with its proven physiological role in the milk ejection reflex. OT effects are not manifested through effects on cell remodeling. Observed effects of OT administration on reproductive anomalies are anestrous, the development of corpus-luteum cysts, follicular ovarian cysts, delayed age at puberty, repeated estrous c...
Min Lv1, Hui Gan2, Zhide Ruan1, Huizan Yang1, Rui Wang1, Laiba Shafique3, Huma Naz4 and Huawei Ma1,
...m) with dissolved oxygen concentrations above 5.2 ± 0.3 mg/l, and pH 6.5–8.1. Water flow in the ponds was poor. The amount of feed increased from 0.81 ± 0.35 to 13.42 ± 1.89 g/fish-day throughout the experiment. Samples were collected between mid-March and October 2016, and we monitored the Cd, Cr, and Hg concentrations in gills, large intestines, small intestines, intestine
Yan Xu1, Jia Zhou1, Guangfu Lv2, Yuexin Liu1, Xintong Zhao1, Xin Li1, Doudan Ye2, Xiaobo Qu1,* and Xiaowei Huang1*
...o experiments. Different concentrations of DOX were used to treat H9c2 cells for 6, 12, 24, and 48 h, respectively, to determine the IC50. Different concentrations of PAP were treated to H9c2 cells for 24, 48, and 72 h to determine the effect of PAP on H9c2 cells. The experiment was divided into control group, DOX group, PAP group and DOX+PAP group. Lactate dehydrogenase (LDH) leakage and creatine kinase MB (CK-MB...

Asmaa Abdallah Darwish 

...udies investigated their concentrations in different sheep diseases. So, this paper aimed to assess the antioxidant vitamins (E, A, D3 and C) levels in some sheep physiological and pathological conditions. For this purpose, 200 barki ewes were divided into control group, inflammatory diseases groups (pneumonic group, arthritic group, enteric group), late pregnant groups (normal late pregnant group, pregnancy toxemia group), postpartum groups (normal postpartum...
Khayyam1*, Muhammad Zahid1, Naiz Ali2 and Qadeem Khan3
... and Luteinizing hormone concentrations were higher than normal range in both male and female Grave’s disease patients. Prolactin and serum gonadotropin showed fluctuations in Hashimoto’s thyroiditis patients but in Grave’s disease patients only the gonadotropins showed abnormal values while prolactin was in the normal range. The study suggests that autoimmune thyroid diseases effect the pituitary-hypothalamic axis which needs to be further i...
Afzal Nimra1, Zulfiqar Ali1*, Safdar Sidra 2, Rida Ahmad1
...This study discusses the concentration and possible sources of airborne trace metals in sensitive areas of hospitals, which is to our knowledge, is the first study contributed from Pakistan and its understanding is important in order to improve law enforcement against causative factors. 
...

Muhammad Salim1*, Ayhan Gökçe1, Muhammad Nadir Naqqash1 and Orkun Ersoy

...treated with the highest concentration (2000 mg/Kg). At the highest concentration (2000 mg/Kg), the mortality reached as high as 100% after 2 weeks, while the lowest significant percent mortality (1.00±1.00%) was recorded in the control one week after exposure and only increased to 3.53±0.00% after 3 weeks. Percent weight loss in grain was also determined after 21 days. A significantly higher percent weight los...

Paul Oludare Adetunji* and Safiriyu Idowu Ola 

...ers (semen volume, semen concentration, sperm output, sperm motility and sperm abnormalities) from 26-52 weeks of age and sexual ornaments’ morphometry (Comb Length-CL, Comb Height-CH, Wattle Length-WL, Wattle Height-WH and Inter Pubic Distance-IPD) from 16-24 weeks of age. Data on onset of puberty and semen quality parameters were subjected to Analysis of Covariance (ANCOVA) with body weight at puberty as a covariate while morphometry measurements were ...

Ehsan-Ul-Haque1, Akbar Hayat1*, Muhammad Asim1, Sajjad Hussain2, Muhammad Shakeel Hanif3, Muhammad Zubair1, Muhammad Abdullah Jamil1 and Faheem Khadija1 

...ate incorporated (6% w/v concentration) in wax were used to compare with commercially used fungicide (Thiabendazole). Study was conducted at 4±1 C° with 90±05% relative humidity for 60 days followed by one week of storage at ambient conditions. Least decay incidence 1.2% and 1.8% respectively in Kinnow and Willow leaf was observed in fruit treated with fungicide in wax followed by fruit treated with Potassium sorbate in wax (2.8% and 3.6%). T...

A. S. M. El-Nuby1† , S. A. Montasser2 and I. A. El-Khadrawy

Control of root-knot nematodes using wild plants colonized Sinai, Egypt
...acantha (60.4%) at stock concentration. The addition of extracts as soil drench was better than spraying them. The stock solution showed the highest reduction in nematode reproduction but the differences between 100% and 75% concentrations were non-significant. Our finding offers a non-poisonous tool that can insert in combating nematode programs especially for small-holder growers colonized Sinai Peninsula.

...

 Tajwali Khan1*, Ihtzaz Qamar1, Feroz Shah2, Kareem Akhtar2, Rehman Akhtar3

MODEL FOR FUEL DROPLET EVAPORATION IN COMBUSTION CHAMBER OF LIQUID PROPELLANT ROCKET ENGINES
...engine where fuel
concentration away from droplet is not zero. A code is generated and applied. The results of the model show that
fuel droplet diameter, combustion chamber pressure, chamber diameter, mass flow rate and droplet relative velocity
with hot gas has significant effects on the life of droplet and characteristic length. Effects of chamber pressure and
diameter are more significant for larger droplet size.

...

Daulat Khan1*, Khizar Azam2, Noor Muhammad Khan1, Muhammad Israr1, Naeem Ejaz3 

ASSESSMENT OF WASTEWATER FOR DISPOSAL IN ACCORDANCE WITH NEQS CRITERIA AND ITS REUSE FOR IRRIGATION WITH DILUTION
...t or waste in an amount, concentration or level which is more than the
National Environmental Quality Standards(NEQS)”. Even in the presence of this act there are many housing societies
and Industrial zones, which are continuously disposing off their untreated wastewater to the natural water bodies. In
this study Laboratory investigations on 5 composite samples were carried out. Total of 19 parameters were analyzed
including T...

Muhammad Shahzad Khattak*1, Barkatullah1, Ayesha Aziz1, Jamil Ahmad2, Mohammad Sharif3, Mukand Singh Babel4, Muhammad Fahad5

IMPACTS OF CLIMATE CHANGE ON CROP WATER REQUIREMENT UNDER MULTI - REPRESENTATIVE CONCENTRATION PATHWAYS DURING MID-CENTURY: A CASE STUDY OF D. I. KHAN

Shabana Rafique1, Shahnaz Parveen Khattak1, Tanveer Hussain2, Faiza Tauqeer1, Bashir Ahmad3 

EVALUATION OF FLEXURAL RIGIDITY AND ABRASION RESISTANCE OF POST AND META-FINISHED PIGMENT DYED P/C FABRICS
...ers i.e. finish type and concentration were taken in to consideration along different modes
of application on the abrasion resistance and flexural rigidity of fabrics. As regards the type of finish, the effect of
U-V absorber and Pekoflam OP flame retardant (organic phosphorous compound) on fabric stiffness was found to be
advantageous, providing it a soft texture. The post-application of fluorine based liquid water repellent finish (NUVAF...

Hamza Tariq1, Imran Hayat1, Saima Rafiq1*, Aqsa Qayyum2 and Sohrab Qayyum1 

...rved with increasing the concentration of autumn olive in the beverage blends while a decreasing trend was observed for pH, reducing sugars, non-reducing sugars and total sugars. Organoleptic parameters such as color, flavor, taste and overall acceptability significantly (p < 0.05) influenced. The TSS, acidity, reducing sugars and total sugars content increased whereas pH, ascorbic acid, antioxidant activity, total phenolics and non-reducing sugar content d...

Sajjad Haydar*, Zunaira Asif*, A. A. Bhatti**, Obaidullah Nadeem***, Ghulam Hussain*, Nadeem Abbas*

EFFICIENCY OF WASTEWATER TREATMENT PLANT IN THE PUNJAB TANNERY SECTOR: A CASE STUDY OF DADA TANNERY
...w wastewater showed high concentration of organic matter
and Chromium. Phosphorous concentration was found deficient for satisfactory biological treatment. WWTP showed
overall removal efficiency of 88.81, 84.54 and 62.31% for total suspended solids (TSS), five day biochemical oxygen
demand (BOD) and chemical oxygen demand (COD), respectively. Whereas, the mean effluent

 Muhammad Irfan*, Saeed Gul*, Mohammad Younas*, Babar Nawaz*, M. Ishfaq*, M. Amir Durrani*, Abid khan*

FERMENTATION OF SUGARCANE MOLASSES BY SACCHARAMYCES CEREVESIA: EFFECTS OF OPERATING PARAMETERS ON ETHANOL PRODUCTION
... time, molasses solution concentration and its pH during ethanol recovery from sugarcane molasses has been investigated. The interaction effects, optimum conditions, and percent contribution ofeach factor were determined statistically. The maximum etha...

Nisar Mohammad*, Mohammad Mansoor Khan* Noor Mohammad*

CHARACTERIZATION OF TALC-CARBONATE ROCK OF MINGORA EMERALD MINES, DISTRICT SWAT, KHYBER PAKHTUNKHWA, PAKISTAN
...ch characterization/ pre-concentration investigations study was done for low-grade talc- carbonate rock of Mingora emerald mines located in District Swat, Khyber Pakhtunkhwa (KP),

Shahida Nasreen Zakir*, Samina Siddiqui**, Nasreen Ghaffar*

MAJOR ELEMENTS CONCENTRATIONS IN CALCAREOUS SOILS
...luate the major elements concentrations in soils derived from various parent materials and then to classify such soils in calcareous, moderate and non-calcareous soils. Soils were analyzed for total content of Al, Ca, Fe, K, Mg, Na, P, Si and Ti with Atomic Absorption Spectrophotometery and UV-VIS Spectormetry. Total Ca content in loess soils is 12% thus categorized in calcareous soils whereas alluvium and piedmont alluvium soils with Ca content ranged from 0....

Hazrat Hussain*, Muhammad Ibrahim**, Navid Ahmed***, Zia ul Haq**, Bashir Alam***

ASSESSMENT OF SEDIMENT TRANSPORT LOAD FROM GOAML RIVER AND ITS IMPACT ON THE GOMAL ZAM DAM LIFESPAN
... sediment load, sediment concentration and sediment quality have been estimated from the actual data and are shown by histograms and bar graphs. The relations developed are applicable only to the Gomal River system. It is hoped that the study will invite interest and attention of planners, researchers, and all those concerned with the problems and menace of soil erosion, sediment load concentration in hyd...
Zarish Yaqoob1, Saleema Bashir Shams2, Gaitee Joshua2 and Bibi Nazia Murtaza2,3*
...sent study describes the concentration of heavy metals in the water and different tissues of native fish collected from three different areas. The highest concentration of Cr (1.451 µg L-1) and Co (0.325 µg L-1) was measured in the river water at Balloki. However, Cd (0.981µg L-1)and Zn (1.239 µg L-1)were contributing maximum contamination at confluence of Deg Nallah a...

 Amir Naveed1, Unsia Habib1, Fouzia Parveen2, Syed Naveed Ul Hasan1, Muddasar Habib1, Irfan Shah1

AN INVESTIGATION OF HEAVY AND TRACE ELEMENTS IN COAL DEPOSITS OF MAKARWAL PAKISTAN AND THEIR POSSIBLE IMPACTS ON SURROUNDING WATER-CASE STUDY
...oal samples had the high concentration of Ni, Cr, As, and Cd. However, the coal samples from Mk-C had higher contents of Cu and Pb. Whereas, Fe was abundant in Mk-B coal samples. These results can be used as a helping tool for the prediction of environmental risks associated with coal mining in Makarwal area. Further, the water samples of Makarwal area were also analyzed and found to be contaminated with heavy metal pollutants.

...

Hina Imtiaz1*, Salma Khalid2, Kashaf Riaz1, Muhammad Arshad Ullah3 and Zubair Rehman

...ssessment of heavy metal concentrations i.e. Lead (Pb), Copper (Cu), Nickel (Ni), Chromium (Cr), Zinc (Zn), Mercury (Mg) and Cadmium (Cd) were carried by Atomic Absorption Spectrophotometer. Significant concentrations of heavy metals were detected except Cu, Ni and Zn. Assessment regarding the types of waste generation, diseases among the residents and their opinions about the recycling and proper waste management were carri...
Fang Liu*
...e show that FGF10 exerts concentration dependent functions in angiogenesis and hypothalamic axon guidance. At lower concentration of 100ng/ml, FGF10 promotes angiogenesis in chorioallantoic membrane endothelial capillaries (CAMs), but FGF10 does not alter the endothelial growth significantly at higher concentration (500ng/ml, P>0.05). Simultaneously, FGF10 exerts a graded action, at low...

Ghulam Murtaza1, Ghulam Sarwar1*, Muhammad Ashraf Malik2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1 and Sher Muhammad3 

...C, and SAR and enhancing concentration of organic matter, phosphorus and potassium. However, T3 (water of EC 3 dS m-1) increased soil electrical conductivity, pH, SAR, and lowered organic matter, phosphorus and potassium concentration. Data were statistically analyzed by statistix 8.1 ANOVA approach along with Tukey’s test (HSD) at probability level of 5% for comparing treatments significance.  

...

Haiyan Yang1, Hongxia Liu1, Wenlong Wu1*, Weilin Li2 and Lianfei Lyu

...lectrolyte leakage (EL), concentrations of photosynthetic pigments, protein, soluble sugar, hydrogen peroxide (H2O2), malondialdehyde (MDA), ascorbate (AsA) and reduced glutathione (GSH), activities of superoxide dismutase (SOD) and peroxidase (POD) in leaves of ‘Hull Thornless’ were investigated. In the treatment group, water stress significantly increased EL, and the accumulation of photosynthetic pigments, protein, soluble sugar, H2O2 and MDA. A...

Syeda Farzana Bibi* and Siraj-ud-Din

Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay
...activity. At higher concentration (100-1000 μg/ml) the leaf and bark ethanolic extracts showed cytotoxicity and at lower concentration (10 μg/ml) very low cytotoxic effect was observed (Figure 4). The LC50 values for crude leaf extract were found to be 5.34 and for that of stem bark extract, it was 7.44 ug/ml. This study manifests that the selected plant can be helpful in the preparation of novel ph...

Kamran Ghasemi1*, Mostafa Emadi2, Asghar Bagheri3 and Mehdi Mohammadi1

Casing Material and Thickness Effects on the Yield and Nutrient Concentration of Agaricus bisporus
...phosphorus and potassium concentration of treated mushrooms but nitrogen concentration was affected by casing layer soil materials and thickness. The lowest nitrogen concentration was in 4 cm depth of CP casing layer that was not significantly different with 2 cm depth of CP casing layer. As a general conclusion, CCS in depth of 4 cm can be the best choice for casing but in ...

Mazhar Abbas1*, Kishwar Jam1, Rashid Iqbal Khan1, Muhammad Zafar-ul-Hye2, Tariq Rafique3 and Zahid Mahmood

...lant products at variant concentrations showed an increase of antioxidant activity by upgrading superoxide dismutase activity (64.42 U mg-1 protein), peroxidase activity (1.90 µmol min-1 g-1 protein), catalase activity (37.81 nmol-1 g-1 protein) and protein (6.28 mg g-1 fresh weight) contents. These commercial plant products based on amino acids enriched macro and micronutrients as well as acetyl-salicylic acid combined with ascorbic acid can be used car...
Anum Feroz1, Abdul Rauf Shakoori2 and Farah Rauf Shakoori1*
...xic effect of sub-lethal concentration (LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain (Lab-S) and deltamethrin resistant population (GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the a...
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2,  Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
...s purpose, two different concentrations 30 µl and 60 µl crude as well as ethanol extract of Aloe Vera were used. During this experiment, total 50 wound samples were collected from donkey wounds. All 50 samples were found contaminated with different bacterial organism, among all those 45, 35, 33, and 25 were recorded positive for S. aureus, E. coli, Shigella and Salmonella spp respectively. However, out of 50 sample, 34 (68%) were foun...

Muhammad Ismail1, Tufail Ahmad2, Shamsher Ali2*, Shahid Ali1, Nur Ul Haq1 and Naveedullah3

Heavy Metals (Pb and Ni) Pollution as Affected by the Brick Kilns Emissions
... samples showed that the concentration of  Pb and Ni in chimney A and B as 2.90 and 1.8, 13.11 and 14.7 mg kg-1 respectively. The concentration of Pb and Ni at this stage is not in toxic range however with the continuous addition may pose a severe threat in future. Similarly, soil sample analysis revealed AB-DTPA extractable Pb and Ni for chimney A and B as (0.11- 0.13, 14.2 and 15.0 mg kg-1) respectively. Here again th...

Adnan Arshad1*, Huma Qamar2, Ristina Siti-Sundari3, Zhang Yue1, Muhammad Zubair2, Muhammad Ali Raza4, Muhammad Habib-ur-Rehman5 and Lizhen Zhang1*

Phenotypic Plasticity of Spineless Safflower (Carthamus tinctorius L.) Cultivars in Response to Exogenous Application of Salicylic Acid under Rainfed Climate Conditions
...lower cultivars and five concentrations of salicylic acid (SA) in three replications was conducted. The safflower promising genotypes viz; C1 (L16358), C2 (L16378), C3 (L26748), C4 (L26754), and C5 (L16385) were tested against SA1: 0 mM, SA2: 0.25 mM, SA3: 0.50 mM, SA4: 0.75 mM, and SA5: 1.00 mM salicylic acid concentrations. The result indicated that most responsive cultivar to rainfed conditions was C3 (L26748) under SA4 (...

Safina Naz1*, Muhammad Akbar Anjum1, Bushra Siddique2, Syed Atif Hasan Naqvi3*, Sajid Ali1, Hassan Sardar1, Sakeena Tul Ain Haider1, Muhammad Asif Zulfiqar4, Hajra Azeem3, Sibghat Ullah5, Shah Pasand6 and Zehri Khan5

Effect of Sewage Water Irrigation Frequency on Growth, Yield and Heavy Metals Accumulation of Tomato and Okra
... revealed that the metal concentration high in tomato and okra plant parts with decreasing irrigation intervals of sewage water.

...
Chao Zhao1,2, Sigang Fana2 and Lihua Qiua2,3,*
...ia, nitrite, and nitrate concentration), heterotrophic bacteria, and vibrios during different P. monodon stages(nauplii, zoea, mysis stage, postlarvae). In ponds 1, 2, 3 and 4, the survival rates were significantly lower but the nitrite concentration and vibrio numbers (before mysis stage) were considerably higher than those in other ponds (P < 0.05), indicating that nitrite and vibrios influenced P....

Amit Sharma1* and Pankaj Sood2

Sonographic Assessment of Ovarian Follicular Dynamics during Breeding and Non-Breeding Season in Gaddi Goats
... season. Average mean P4 concentration was 0.30±0.04 ng/ml throughout the period of observation during NB whereas, peak P4 concentration of 11.89±1.55 ng/ml was observed at Day 14 of estrous cycle during B season. Significant difference between average daily counts of small, medium along with daily total number of visible follicles were observed during B season. In conclusion, ovarian follicular growth in Gaddi...

Abdul Jalal, Abdur Rab* and Naveed Ahmad

Aloe Vera Gel Coating Retains Persimmon Fruit (Diospyros kaki) Quality during Storage at Room Temperature
...ations and Aloe vera gel concentrations significantly decreased the loss of persimmon fruit quality during storage at room temperature. Storage at room temperature (26±2 °C) resulted in significant decline in fruit firmness, percent acidity, and ascorbic acid content with a concomitant increase in total soluble solids (TSS), organoleptic quality (Taste) score and decay incidence. Coating of persimmon fruits with 30% Aloe vera gel retarded the storag...
Huma Abbas1, Muhammad Azhar Iqbal2, Muhammad Kamran3, Muhammad Umar Shahbaz3*, Haseeb Ullah Kamber1, Nazir Javed1, Muhammad Junaid1, Hira Abbas4 and Muhammad Ehetisham ul Haq3
Evaluation of Advanced Mung Bean Germplasm against Cercospora Leaf Spot and its In-vitro Management by Different Fungicides
...10, 50, 100, and 200 ppm concentration against C. canescens using poisoned food technique. Score at 100 ppm was found most effective (93.03%) against the disease as compared with other fungicides. From present research, it is devised that 16051 line may be used for future breeding program and Score at 100 ppm may be evaluated for field trials in future studies.

...

Shahid Nadeem1*, Taj Naseeb Khan1, Mushtaq Ahmad1, Adnan Younis2 and Iram Fatima3

Influence of Plant Growth Regulators by Foliar Application on Vegetative and Floral physiognomies of Gladiolus
...ngth (77.67cm) at 1.75mM concentration of GA3. The Effect of SA found worthy as it enhanced the floral growth and decrease the number of days towards blooming. Moreover, the days to spike emergence, days to bud color and days to floret open were decreased (96.47, 101.20 and 105.77 respectively) with 1.75mM salicylic acid. However, the results showed the positive impact of GA3 and SA on vegetative as well as floral physiognomies of gladiolus plants.

...

Zahir Shah1*, Saima Munawar1, Ihsan Ullah2, Taif Shah3, Haq Aman Ullah1, Saqib Nawaz1, Farhan Anwar Khan1 and Manoj Kumar Shah4

Combined Physico-chemical and Analgesic Effects of Electroacupuncture Plus Clonidine in Goats
...ntly lower serum glucose concentration was noted in EA plus 10µg/kg of clonidine treated goats at 15-60 min than those treated with clonidine alone. Creatinine, blood urea nitrogen, alanine/aspartate aminotransferase activities, and hematological variables for differently treated groups were insignificant. In conclusion, EA combined with a low dose of 10µg/kg of clonidine provided better analgesia in experimental animals.

...

Tahseen Ullah* and Noor ul Amin

In Vitro Antibacterial Activity of Medicinal Plant Extracts
...dford, UK. Two different concentrations (low, 5 mg/ml and high extracts, 300 mg/ml) of these plants were evaluate against two different bacterial strains i.e. Bacillus subtilis and E. coli using disc diffusion method to uncover antibacterial potential of the plants extracts. The antibacterial investigation was performed in three replicates using disc diffusion technique using Nutrient Agar Media for the growth of B. subtilis and E. coli bacteria. The ANOVA for...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...0%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at 4&d...
Gulzhan Aubakirova1*, Zhanat Adilbekov2, Assylkhan Inirbayev2, Assel Zhamanova2 and Aibar Akhmetov3
...s within the permissible concentration, however, a decreased permanganate oxidation was noted, which indicates a satisfactory state of water quality. Widespread infection of fish with postdiplostomatosis was revealed in assessing fish safety; in rare cases, diplostomosis and ligulosis were detected. The incidence of fish with bacteriosis was not observed, with the exception of isolated cases of aeromonosis. Exceeding the maximum permissible
Aatif Amin1,*, Zakia Latif2, Arslan Sarwar1, Basit Zeshan1 and Mushtaq A. Saleem1
...ce level against various concentrations of HgCl2. Bacterial isolatesAZ-1, AZ-2 and AZ-3 showed resistance up to 40 μg/mL of HgCl2 and mercury sensitive (HgS) isolate ZA-15 was taken as a negative control. 16S rDNA ribotyping and phylogenetic analysis were performed for the characterization of isolates as Bacillus sp. AZ-1 (KT270477), B. cereus AZ-2 (KT270478), B. cereus AZ-3 (KT270479) and Ente...
Asim Faraz*
...a, Mg, Cu, Zn, Fe and Mn concentrations were found to be significantly different (P<0.05) among calf groups in IMS, SIMS and EMS.
...
Abida Parveen1, Sajid Aleem Khan1, Nazir Javed1, Anam Moosa1*, Khushboo Javaid1, Hina Safdar1 and Asma Safdar2
...th SK at three different concentrations and time intervals. After 12-h ‘S’ concentration of aqueous MOextract + ethanolic MO extract + SK produced 100% juvenile mortality while the treatment MO + SK caused the least juvenile mortality of MI. Juvenile mortality was increased in a time-dependent manner. Aqueous MO extract + ethanolic MO extract + SKproduced 100% egg hatching inhibition at standard ‘S’ <...

Dalal H. Sary and Rama T. Rashad*

A Comparative Study on the Impact of Compost, Humate, and Silicate on the Nutritional Characteristics of Calcareous Soil Cultivated by Soybean
...ave almost decreased the concentration (mg kg-1) of Cu, Fe, Mn, Zn, and Si in soybean seeds significantly by increasing the application rate from 50 to 100 %. Potassium silicate was the most effective Si-source in this study due to its content of readily soluble Si in soil solution. Silicon uptake can partially control the availability and uptake of some nutrients from soil. Soil may be enriched by versatile advantages upon application of K-H and K-Si but the ...

Asif Ali Abro1* and Iqbal Ahmed Panhwar2

Impact of Various Factors on Crop Diversification Towards High Value Crops in Pakistan: An Empirical Analysis by using THI
...ed to check the level of concentration or diversification in the structure of crops in Pakistan. The values of the Herfindahl index obtained as a result of the analysis indicate a high level of crop concentration and need to be diversified towards high value crops. The ARIMA time series model (1,1,1) was used to study the impact of various factors on the Transformed Herfindahl Index (THI) of diversity. To check the impact of...

Safiullah1, Azmat Ali Awan2, Mohammad Ilyas3, Fahim Ullah Khan3, Arshad Iqbal3*, Muhammad Adil3 and Asad Ullah1

Effects of Different Concentrations of Naphthalene Acetic Acid on Rooting of Various Olive Varieties
...dy the effect of various concentrations [0 ppm (control), 1000 ppm, 2000 ppm and 3000 ppm] of Naphthalene Acetic Acid (NAA) on rooting of various olive varieties (Hamdi, Nocellara and Sevallino) an experiment was conducted at Agricultural Research Institute (ARI) Tarnab, Peshawar during 2016. The trial was performed using two factorial RCBD. Cuttings were collected from mature olive orchard. The cuttings were treated for ten seconds in required
Wajahat Azeem1,*, Tariq Mukhtar1 and Tooba Hamid2
... was the maximum at 100% concentrations of both the agents while the minimum inhibition and mortality was obtained at 25% concentration. No statistical difference was observed between T. harzianum and A. indica in causing hatching inhibition and larval mortality. Numbers of galls, egg masses and reproductive factor were reduced significantly as a result of A. indica and T. harzianum applications. ...

Muhammad Nazar1, Chaudhary Muhammad Ayyub2, Mujahid Ali3*, Malik Abdul Rehman4, Shoib ur Rehman5 and Saqib Ayyub2

Amelioration of Thermo-Tolerance Ability in Spinacia oleracea L. Cultivars by Foliar Application of Chitosan
...application with optimum concentration may help to improve the production potential of spinach cultivars in areas hit by temperature stresses.

...
Shakeel Ahmed* and Sarwat Jahan
...tervals. Plasma T and GH concentrations were determined by using specific Enzyme Immunoassay (EIA) kits. 48 h fasting significantly (P<0.001) decreased plasma T levels but it did not cause any significant (P>0.05) change in plasma GH levels compared to normal fed monkeys. NMS injection induced a significant increase (P<0.05) in T and GH concentrations compared to saline treated animals suggesting the possible involv...

Syeda Farzana Bibi* and Siraj ud Din

Unraveling the Bioherbicidal Potential, Elemental Analysis and Nutritional Evaluation of Crataeva adansonii Dc Leaf and Bark
...inhibition at 1000 μl concentration, and at the same concentration, the bark extract inhibited the frond growth by 40%. The pharmacognostic evaluation of this medicinal drug will be supportive in the detection of adulteration and quality control. Due to the herbicidal potential, it can be used in the agricultural industry. For future studies, it is suggested to understand the mode of action of its active compounds through...
Tehreem Zahra1, Muhammad Irfan1, Muhammad Nadeem2, Misbah Ghazanfar1, Qurratulain Ahmad3, Shaukat Ali4, Farzana Siddique5, Zarina Yasmeen6, Marcelo Franco7
...variables like substrate concentration, peptone and KH2PO4 were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity was obtained after 96 hours of incubation with optimum conditions of media containing 5% substrate concentration, 0.05% peptone and KH2PO concentration o...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
...degradation at different concentrations and durations. However, inhibition of autophagy by 3-MA rescued the survival of Hela cells. This study demonstrated that the potential effect of spermidine on decreasing the survival of Hela cells is attributable to autophagy induction.
...
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
...ed that the average eDNA concentration of Sepiella japonica extracted by three kits were (4.62 ± 0.72) × 105, (3.83 ± 0.56) × 105, and (2.35 ± 0.27) × 105 copies/300 ml, respectively. The eDNA concentrations by AxyPrep DNA Gel Extraction Kit were about 83% of that extracted by DNeasy Blood and Tissue Kit. However, extracting cost of an eDNA sample...

Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3

...evels of plasma FSH. The concentrations of testosterone and LH were found to be significantly reduced in kisspeptin treated groups of 1µg dose (p<0.01) and 1ηg dose (p<0.05) whereas the dose of 10ρg had shown no significant change. Kisspeptin doses of 1ηg and 1µg lead to significant reduction in quantity of elongated spermatids and daily sperm production (p<0.05; p<0.001), step 7 spermatids (p<0.05; p<0.001), type A sp...
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...plant species, botanical concentration and exposure time. The extracts of Azadirachta indica (neem) and Nerium indicum (oleander) appeared to be most effective against the termites with minimum LC50 (6.35 and 10.38%, respectively) and LT50 (12.11 and 17.49 h, respectively) values, followed by the extract of Gardenia jasminoides (gardenia). While the extracts of N. indicum (oleander) and Dodonaea viscosa ...
Mehwish Saleem Khan
Evaluation of Changes in Zinc Levels in Patients Suffering from Cancer
... evaluate the serum zinc concentration. The sample zinc level is estimated by using the spectrophotometer in laboratory. The level of zinc in cancer patients was observed lower than the optimum value. Male have much lower concentration as compare to females, therefore male are more susceptible to cancer than female. In the nutshell Zinc is an effective anti-inflammatory and antioxidant agent, including as a natural cancer tr...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...er biomass and glycoside concentrations through greater system productivity. Hitherto, there was no significant study about the effect of far-red LED induction to improve the steviol glycoside content of Stevia plants, particularly at the molecular level. This research was therefore performed in the TIS RITA® bioreactor system to evaluate the effect of far-red LED induction towards biomass growth, multigene expression related to steviol glycosides, and its...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3

...ccp Pakistan strain. The concentration for each drug was measured distantly depending on high concentration available in the market and was then used in various concentrations ranges from higher to lower i.e. moxifloxacin 80-0.156 µg/ml, levofloxacin 250-0.488 µg/ml, ciprofloxacin and enrofloxacin 50-0.097 µg/ml. The resultant effective antimicrobial by minimum inh...

Zubia Rahim1, Gulnaz Parveen1*, Salma Gul2 and Khushnood-ur-Rehman3

Ameliorating Effects of Salt Stress (KCl, NaCl) on Growth and Germination Parameters of Pearl Millet (Pennisetum americanum)
...of NaCl + KCl in various concentrations on the growth and germination of Pearl millet (Pennisetum americanum), locally named as Bajra. Five seeds sown in petri dishes to observe the radical, plumule length and germination percentage after treatments to 100, 150, 200 and 250mM concentration of above mentioned salts. Control petri dishes/ pots were irrigated with distilled water. The experiment was extended to study growth (nu...
Arifa Khan1*, Arsalan Bashir1, Shazia Erum2, Jabar Zaman Khan Khatak1 and Aish Muhammad3
Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation
...e shoots (7.33) when the concentration of BAP and IAA was 2.5 and 1 mg L-1, respectively. Alike, Wiallium-8818 hybrid produced the longest shoot (12.20 cm) with more leaves (6.23) at 1 mg L-1 of each BAP and IAA. But Pisang variety produced maximum fresh weight (15.45 g) with a greater number of roots at 5 + 1 mg L-1 of BAP + IAA, respectively.

...

Muhammad Yasir*, Mansoor ul Hasan, Muhammad Sagheer, Amer Rasul, Rameesha Amjad Ali and Habib ur Rehman

Evaluation of Spinosad Applied to Grain Commodities for the Control of Stored Product Insect Pests
...maize, rice and oats) at concentrations of 0.25, 0.50 and 1 mg Kg-1 under laboratory conditions maintained at 28 ± 2oC, 65 ± 5% RH and continuous darkness. Seven bioassays were conducted by releasing the insects on treated commodities after different post treatment periods (0,1, 2, 3, 4, 5 and 6 months). The mortality of three insect species was recorded at tested concentrations in all the treated commodities a...

Muhammad Jahanzaib1*, Nazakat Nawaz1, Muhammad Arshad1, Shehzadi Saima4, Muhammad Suhaib2, Muzammil Husain3, Haris Khurshid1 and Shahid Ali Khan1

Effect of Temporal Application of Gypsum on Mineral Uptake and Economically Important Morphometric Traits in Groundnut (Arachis hypogea L) under Rain-fed Conditions
... and minerals (Ca, K, P) concentration in root and shoot have been examined. A newly released Pothowar groundnut variety (Variety name) was grown in 3 replications with 5 m row length using a randomized complete block design. Five different treatments were applied in two split doses i.e. T1 (0 %:100 %), T2 (25 %:75 %), T3 (50 %:50 %), T4 (75 %:25 %), and T5 (100 %:0 %) at the time of sowing and pegging compared with Check (T0). Results showed significant diffe...

Sajid Ali1, Abdul Basit1,3*, Abdul Mateen Khattak1, Syed Tanveer Shah1, Izhar Ullah2, Noor Alam Khan3, Imran Ahmad1, Kamran Rauf1, Salman Khan1, Irfan Ullah1 and Intizar Ahmad1

Managing the Growth and Flower Production of Zinnia (Zinnia elegans) through Benzyle Amino Purine (BAP) Application and Pinching
...inins and decrease auxin concentrations flourishing the axillary buds. A research study on the growth and production of zinnia flowers through application of BAP (25, 50, 75 and 100 mg L-1) and pinching (2, 4 and 6 leaf stages), was carried out at The University of Agriculture Peshawar, Pakistan. Research analysis showed that zinnia plants treated with 100mg.L-1 and pinched at 6 leaves stage recorded maximum days to flowering, flower persistency, branches, lea...

Noor Ayaz1*, Faiza Aman1, Sidra Saleem1*, Maqbool Rehman1 and Muhammad Fahim2

Olive Cuttings as Affected by Different Concentrations of Indole Butyric Acid
... the effect of different concentrations of indole butyric acid (IBA) on olive cuttings, a greenhouse study was conducted, at Agricultural Research Institute, Tarnab during the growing season 2016. Completely randomized design (CRD) was used with four treatments that were replicated three times. The IBA concentrations used were (T1: control, T2: 1000 mgL-1, T3: 2000 mgL-1, T4: 3000 mgL-1). Data were recorded on days to 50% sp...
Muhammad Shafiq1,2,*,Rajwali Khan1, Ilyas Ali3, Sadeeq Ur Rahman4, Saif Ullah3Shah Jan Mohammad2, Mohammad Jan2 and Jinhu Huang2
... and serum total protein concentration (b) colostrum immunoglobulin level and (c) their respective calves’ serum immunoglobulin and serum total protein concentration. Three breeds of cattle were observed: Jersey, Holstein Friesian (HF) and local Pakistani cow breed Achai. To assess serum IgG, sodium sulphite precipitation technique was used while IgG in colostrum were determined using digital Brix refractometer ...

Ambreen Akhtar Saddozai1, Amer Mumtaz2, Naseem Rauf1, Saeeda Raza2, Nouman Rashid Siddiqui2, Muhammad Naeem Safdar2, Sahar Shibli2, Muhammad Suhail Ibrahim3*, Muhammad Akhtar4 and Muhammad Saad Rehan5

Preparation and Quality Evaluation of Soymilk Carrot Blend
... milk at three different concentrations levels 2%, 4% and 6% and packed in sterilized bottles. The product was kept at two different temperatures (ambient and refrigerated temperature) for 14 days to assess its shelf life. During the storage period the samples were analyzed for different chemical parameters total soluble solids, pH, total carotenoids, protein contents and antimicrobial analysis. On the basis of quality attributes it was observed that plain and...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Sher Muhammad3, Usman Saleem4, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz5 and Aamer Sattar2

Impact of NaCl Toxicity on Yield and Yield Components of Rice (Oryza sativa) Grown in Aridisol
...s more pronounced as the concentration of salt increased in the treatments.

...
Shikang Deng1,2, Yan Jin2, Jing Xu2, Xiufang Zhu2, Pinghai Hu2, Jiao Li2, Li Zhang2 and Jianzhong Tang2,*
...sts were given different concentrations of 5-FU (0, 0.001, 0.01, and 0.1 mmol/L). After 48 h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed ...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...s no difference in semen concentration, sperm motility and useable spermatozoa/ejaculate before 32 wk of age (p>0.05). Feed quantity was correlated positively with sperm concentration and sperm motility. At 50 wk of age, higher sperm motility was observed in males fed with 135.5and 125.5 g/d diet than males fed with 115.5 g/d diet (p<0.05). While feed quantity was correlated positively with semen volume, sperm

Rahat Afza, Muhammad Asam Riaz*, Muhammad Afzal and Muhammad Zeeshan Majeed

Adverse Effect of Sublethal Concentrations of Insecticides on the Biological Parameters and Functional Response of Predatory Beetle Coccinella septempunctata (Coleoptera: Coccinellidae) of Brassica Aphid
...cides at their sublethal concentrations was assessed on the biological parameters and functional response of Coccinella septempunctata. All insecticides significantly reduced the fecundity, TPOP (total pre-oviposition period), larval weight and increased the life span of C. septempunctata relative to control. Thiamethoxam and lambda-cyhalothrin non-significantly reduced the coccinellids larval weight. Type II response was shown by larvae of C. septempunctata i...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz3, Abid Niaz2, Khurshid Ahmad Mufti4 and Muhammad Arif2

Deleterious Impact of Sodium Chloride (NaCl) Toxicity on Chemical Properties of Soil and Ionic Composition of Rice
...late SAR values of soil, concentration of Ca2+, Mg2+ and Na+ were also determined from all soil samples. It was observed from the results that addition of NaCl proved toxic for different chemical properties of soil as values of pH (7.23 to 8.44), EC (2.96 to 13.54) and SAR (2.45 to 11.57) were impaired negatively and in systematic manner with the increase of NaCl concentration in soil and in all treatments when compared with...

Muhammad Umair Hassan1, Muhammad Aamer1, Muhammad Nawaz2, Abdul Rehman3, Talha Aslam3, Ubaid Afzal4, Bilal Ahmad Shahzad3, Muhammad Ahsin Ayub5, Faryal Ahmed1, Ma Qiaoying1, Su Qitao1 and Huang Guoqin1*

Agronomic Bio-Fortification of Wheat to Combat Zinc Deficiency in Developing Countries
...tant ones to increase Zn concentration in wheat grains up to desirable levels. The genetic techniques are costly, and sustainable, however, they are long term and requires large breeding activities and resources. Conversely, agronomic techniques appear to be short term, quick and economic solution to increase the Zn contents to meet human needs. The application of Zn as foliar sprays, seed priming, soil application and soil+foliar application effectively enhan...

Muhammad Farooq1*, Elham Azadfar2, Zohre Bahrami3, Mahniya Sharifi3, A. Shakoor4, Shabir Ahmed5, Iftikhar Ahmed Solangi1, Muhammad Noman Khan6, Memona Siddique7, Naila Ilyas8*, Muhammad Bakhtiar9, Mohamed El-Fatieh Ismael1 and Wang Yunyang1

Optimization of Drying Process of Rambutan by Combined Method of Osmosis Ultrasound and Complementary Drying of Hot Air
...is-ultrasound with 40.5% concentration in Sekanjabin solution then dried in an oven at a temperature of 70°C. The characteristics of solid gain, water and weight loss, shrinkage and rehydration were evaluated. The obtained results showed that by increasing the concentration of weight and water loss, solid gain increased. Accordingly, in a higher concentrated osmotic solution, the osmotic pressure, which is a key factor i...
Muhammad Farooq1*, M. Javed Iqbal2, Rizwan Shukat2, Qayyum Shehzad3, Shabir Ahmed4, Elham Azadfar5, Abdul Saboor6, Ibrar Ahmad7, Iftikhar Ahmed Solangi1, Kaleem Kakar8, Shoaib Khan9 and Wang Yunyang1
Development of Onion Powder by using Egg Albumin as a Foaming Agent by Foam-Mat Drying Process
...s treated with different concentration of egg albumin (0%, 4%, 8% and 12%) as foaming agent and Carboxyl methylcellulose (0.5%) as foam stabilizer and these were dried in hot air tray drier at different temperatures (55°C, 65°C and 75°C) with 3mm sheet thickness of onion foams. Effect of different concentration of foaming agent and drying temperature was studied on moisture and drying rate of onion paste. Increas...
Muhammad Umar1, Muhammad Arshad2, Mubashar Hussain1*, Moazama Batool3 and Muhammad Faheem Malik1
...er than safe limits. The concentration of these metals showed variation in different months of the year. An extensive monitoring of the lake and catchment area is suggested in order to address the threats posed by heavy metals.
...
Xiaopeng Tang1,*, Lei Chen1, Kangning Xiong1, Dun Deng2 and Peng Peng2,*
... treated with increasing concentration of DDGS. Compared to mock-treated group, the average daily gain (ADG), average daily feed intake (ADFI) and feed to meat ratio (F:G) were measured. It was revealed that the addition of DDGS up to 24% in the diet remained ineffective for ADFI, ADG and F:G. However, there a tendency of decrease in ADG was observed when 32% DDGS was added in the feed. Taken together, our results demonstrate that the increase of DDGS in feed ...
Faiza Altaf1, Shamim Gul1,2*, Tasawar Ali Chandio3, Gul Bano Rehman1, Attiq-ur-Rehman Kakar4, Sami Ullah4, Naqeebullah Khan4, Umbreen Shaheen5, Muhammad Naeem Shahwani6, Muhammad Ajmal7 and Misbah Manzoor8
 
 
...manure on the growth and concentration of heavy metals in edible tissues of tomato and lettuce. These biochars were separately mixed with poultry manure as 1:1 ratio on air-dry weight bases. Soil was contaminated with chromite mine tailing debris at 2% amendment rate (980 g soil + 20 g debris). Biochar-poultry manure mixtures were applied in soil at 10% and 20% amendment rates (respectively, 900 g soil + 100 g mixture and 800 g soil and 200 g mixture of poultr...
Javeria Afzal, Muhammad Abid, Faisal Hussain* and Alia Abbas
...m stem extract at 2% concentration. The minimum reduction (0.33%) was observed in the leaf extract of A. viridis at 10% concentration after 48 hours of exposure time. In larval mortality test, maximum larval mortality (33%) was recorded in C. album leaf extract at 10% concentration while minimum larval mortality (0.66%) was noted in Solanum nigrum at 2%
Naveed Akhtar1*, Muhammad Fiaz Khan1*, Sadia Tabassum1
Munawar Saleem Ahmad2 and Khan Dil Badshah3
...ibes effect of different concentrations of cypermethrin on hematological parameters, biochemical components of blood, histopathological changes in different organs and DNA damage in Chirruh snow trout Schizothorax esocinus. WBCs, RBCs, hemoglobin, hematocrit, MCV, MCH, MCHC and platelets were significantly decreased in all treatedgroups compared to control. Monocytes showed significant increase in all experimental groups. Lymphocytes counts significantl...
Muhammad Younis1, Muhammad Irfan-ur-Rehman Khan1*, Mustansar Abbas1Ali Murtaza1, Imran Mohsin2, Muhammad Shahzad3 and Muhammad Zahid Tahir1
...ent and steroid hormones concentrations during estrous cycle of Lohi sheep. Ovaries of nine Lohi ewes were monitored for two consecutive estrous cycles using B-mode trans-rectal ultrasonography during the breeding season (Sep-Nov 2018). Plasma progesterone and estradiol-17β concentrations were determined throughout the cycle using radioimmunoassay. The average length of the estrous cycle in Lohi sheep was 17.0±0....
Kamal Al-Samawi1, Mohamed Al-Hassan2, Hussein Migdadi3,4*, Megahed Ammar5 and Salem Alghamdi3
... serum progesterone (P4) concentrations were assayed using an ELISA kit. Pregnancy detection was performed by US on 23, 35, and 60 days post NM. Serum P4 concentration was significantly higher in pregnant than non-pregnant goats at 15, 23, 35, and 60 days post NM. Relative expression of mRNA of ISG15 and ISG17 was significantly higher in pregnant goats at 7, 15, and 35 days post NM. ISG15 and ISG1...
Muhammad Naeem1,2, Nasir Rajput1,2, Sher Ali3, Asmatullah Kaka2, Dildar Hussain Kalhoro2, Mehvish Rajput2 and Tian Wang1,*
...H than other groups. The concentration of acetate, butyrate and iso-butyrate were almost similar in TRS-II, AH and CWR; valeric and iso-valericwere lower (P<0.05) in CWR and TRS-I than AH and TRS-II, while the propionate and total VFA were higher in AH but similar in CWR and treated rice straw. The in vitro degradibility of DM, OM and NDF were significantly higher for AH and TRS-II while, ADF was higher for AH. Steam treated straws show...

Saleem Maseeh Bhatti1*, Aslam Khan Gadehi1, Inayatullah Rajpar1, Muhammad Nawaz Kandhro2, Mukesh Kumar Soothar1 and Zohaib Ur Rehman Bughio1

 

 

...±0.27 grams). The concentration of Na+ increased up to 3.4 times and Cl- concentration enhanced up to 2.7 times in leaves of spinach genotypes while no profound effects on K+ concentration in leaves was observed. English spinach accumulated significantly more Na+ and Cl-, and less K+ than Sindhi spinach. In soil, the ...
Iftikhar Hussain1, Sardar Azhar Mehmood1, Muzafar Shah2*, Mohammad Salim3, Shabir Ahmed1, Sana Ullah1,Sidra Batool4, Nadia Saeed1 and Khalid Usman1
... sequences showed higher concentration of A+T as compared to G+C. The A+T contents were 53.1% and C+G were 46.8%. In phylogenetic tree the sequences of C. sphinx clustered together with 100% bootstrap support. The relationship between C. sphinx and C. horsefield was supported by 83% bootstrap value. The relationship between C. titthaecheilus and C. horsefield was supported by 98% bootstrap v...
Hafiz Hammad1, Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Abid Niaz4 and Muhammad Arif4
... P accumulation, plant P concentration, P uptake efficiency and PE ratio were recorded using standard procedures and obtained data was analyzed statistically with a statistical software Statistix 8.1 analysis of variance technique and significant of treatments was tested using LSD test at probability level of 5%. The maximum P accumulation (178.37 mg P pot-1), P uptake efficiency (1.90) and PE (phosphorus efficiency) ratio(747.15 g DM (g plant-...

Pakistan Journal of Agricultural Research

March

Vol. 34, Iss. 1, Pages 1-253

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