Sogolo L. Lebelo1,* and Gerhard van der Horst2
...ant lipid inclusions and collagen fibers are also found in the cytoplasm of these cells. These cells are found associated with the spermatogonia type B, pachytene spermatocytes and Sertoli cells. The results from this study show that there are basic similarities in the Leydig cells of the vervet compared to other mammals, humans and nonhuman primates, however, there also seem to be species differences among the mammalian group. The association of these cells w...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...e HS, which can regulate collagen synthesis and deposition. To investigate collagen metabolism in keratinocytes and fibroblasts co-culture under pressure, and study the role of IL-1α and MMP-3 in terms of regulating fibroblasts-keratinocytes collagen metabolism with pressure.Chitosan-gelatin scaffolds were fabricated by vacuum freeze drying. Keratinocytes and fibroblasts were seedi...
Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*
...stepwise digestion by XI collagenase and trypsin was used to isolate MDSCs and were purified by differential attachment technique. Cell morphology were observed using an inverted microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDSCs were induced...
Kun Wang1,2, Yinglin Cui2, Xu Zhao2 and Changjiang Hu1*
...istration of stereotaxic collagenase type VII into the caudate nucleus. XN (at a high dose and a low dose of 3.60g·kg-1 and 1.80g·kg-1, respectively) was administered via enema. The detection of neuronal apoptosis was measured by TUNEL assay. Using immunofluorescence, the expression of claudin-5, ZO-1 and VE-cadherin was detected. The expression of DNA methyltransferase 3b (DNMT3b) and Matrix metalloproteinase-9 (MMP-9) was ...
Li-na Li1, Sheng Li2, Ping Gui3, Jing-hui Li1, Fen Ai4,* and Li-li Cai1,*
...roliferation and reduced collagen deposition as measured by CCK-8 assay, Sircol assay, qPCR and western blot. MSCCM also reduced the expression of the pro-fibrotic TGF-β1 and increased the expression of anti-fibrotic IFN-γ at both mRNA and protein levels. Furthermore, we found that MSCCM inhibited the activation of NF-κB signaling pathway induced by irradiation as well. Our data suggest that MSCCM could reduce irradiation-induced TGF-β1 p...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
... proline residues in the collagen chain. The α subunit (P4Hα) of the P4H α2β2 tetramer contains the catalytic site, whereas the β subunit (PDI) maintains the solubility and activity of the α subunit. In this study, two plasmids were used to produce an active human P4H tetramer (rhP4H) in Pichia pastoris. The P4Hα was cloned into pPICZαA, and PDI was cloned into PHIL. Real-time PCR showed that the P4H gene wa...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF group and the control group with regard to heart rate, blood pressure, serum creatinine, BUN, LDH, cytokines and urinary electrolyte levels. Furthermore, monocyte and neutrophil apoptosis, tissue luminol, lucigenin, malondialdehyde and collagen
Arifa Savanur1,*, Tallat Naz1,2, Tayyaba Hamid1,3, Syed Abid Ali4, Mian Jahangir1,3 and Muhammad Abdul Azeem4
...s are less embedded in a collagen matrix. A significant difference between both types of muscle strips was observed in the rate of force re-development and change in their length. Thus, the present study provokes the need for quantitative histological and histo-chemical studies on the non-contractile proteins of Uromastix smooth muscle.
... 
Rafiullah Khan1, Waseem ur Rahman1, Misbah Ullah2, Kamran Afaq3, Muhammad Amjad1, Sakhi jan1
AGE EFFECT ON THE MECHANICAL PROPERTIES OF HIP JOINT BONE: AN EXPERIMENTAL INVESTIGATION
...s matrix binder and soft collagen as reinforcing fibers. As bone supports all the organs of a body so its
mechanical behavior is of prime importance. The mechanical properties of bone varies with age. In this paper, the
effect of age on various mechanical properties of hip joint bone was investigated experimentally. For this purpose
hip joint bone specimens of male bovine of different age groups were tested for fracture toughness, young mo...
mechanical behavior is of prime importance. The mechanical properties of bone varies with age. In this paper, the
effect of age on various mechanical properties of hip joint bone was investigated experimentally. For this purpose
hip joint bone specimens of male bovine of different age groups were tested for fracture toughness, young mo...
Olga Mikhailovna Blinnikova1*, Vadim Anatolyevich Babushkin1, Lyudmila Gennadievna Eliseeva2 and Galina Severyanovna Usova3
Modeling a Formulation and Assessment of the Consumer Properties of the Special Purpose Starch Drink
...sing of a hydrolyzate of collagen as a component of the enriched foodstuff opens unlimited opportunities of production of the goods for functional, medical preventive and medical purposes, capable to fill the shortage of collagen and to stimulate the human body to produce its own protein. Drinking kissel is a perspective product type for enrichment the CCR (Central Chernosem Region’s) fruit and berry’s by
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
Qiang Tang1 and Qianli Tang2*
..., and width of subdermal collagen fibers also decreased (p<0.001), inflammatory factors TNF-α, IL-6 and IL-1β in skin tissue increased (all p<0.001), and expression levels of Ang-1 and Tie-2 proteins in skin tissue decreased (both p<0.001). Compared with model group, thickness of epidermis, dermis and collagen fibers, and expression levels of Ang-1 and Tie-2 proteins in skin tissue in...
Imran Tarique1*, Muhammad Ghiasuddin Shah1, Illahi Bux Kalhoro1,
Zaheer Ahmed Nizamani2, Mansoor Tariq 2, Jamil Ahmed Gandahi1,
Saqib Ali Fazlani3 and Benazir Sahito3
...hich consist of elastic, collagen and reticular fibers. Superficial dermal papillary layer was located beneath the epidermis, whereas dermal reticular layer deeper above the hypodermis. The papillary and reticular dermis was well developed in adult male age group with abundant areolar and dense irregular connective tissue all skin regions. Skin appendages (sebaceous and sweat gland, and hair follicle) was well developed and distributed in papillary and ...
Zheng Yan1,2,*, Shuang Liu2, Ai-Qing Liu2 and Hai-Yan Wang2
...traions of three type of collagens, hyaluronic acid and hydroxyproline in skin tissue were also determined. The results indicated that the oral administration of elastin peptide greatly improved the skin elasticity, accompanying with significantly upregulated expression of hyaluronic acid and hydroxyproline. In addition, the contents of collagen in animal skin were also significantly increased, especially Type III and IV
Sozan A.A. Ismaeil1*, Hassan Emam2
...ew amounts of reticular, collagen, and elastic fibers with silver, Crossman, and Orcein stains respectively and faint positive reaction with PAS while in the liver of Ibis was vice versa. This study aims to understand normal gross anatomy and histology of the liver in Young Homing Pigeons and Cattle Egrets. It can be concluded that the presence of gall bladder and absence of it and habit of feeding significantly affected the gross and histological structure of...
Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4
...oped nanobioactive glass/collagen/chitosan composites scaffolds with osteoblast mineralization of normal rabbit bone marrow mesenchymal stem cells (rBM-MSCs) and cancer osteosarcoma cells. The non-cellular in vitro bioactivity test was performed in simulated body fluids for periods 1, 3, 10, 20 and 30 d by measuring the calcium and phosphate ion concentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiated osteoblast cells de...
Jocelín Selene Sánchez Cisneros1, Braulio Alejandro Fuantos Gámez1, Martin Herrik Ramón Kane1, Laura Miranda Contreras2, Camilo Romero Núñez2*
...synthesis or assembly of collagen. This case describes a young albino Burmese python that presented with dry-looking skin, multiple folds and wounds. Skin samples were taken for histopathology and compared with a healthy specimen of the same species and colour characteristics. The diagnosis was cutaneous collagen dysplasia suggestive of cutaneous asthenia. This is the first report of cutaneous asthenia in a baby albino Burme...
Dhiya Altememy1, Mohammad Darvishi2*, Samira Shokri3, Saber Abbaszadeh4
...to determine the rate of collagenization, wound, and extracellular matrix. After the treatment, the size of the wound in the Tio2 N.P. synthesis by Origanum vulgare, Tio2 N.P. synthesis by carvacrol, methicillin, Tio2 N.P., and alginate pad groups of diabetic rats and alginate pad group of non-diabetic rats were determined 7.03 ± 1, 11.12 ± 1, 10.72 ± 1 ± 15.48 ± 1, 11 ± 1, and 6.35 ± 1 mm, respectively. The ser...
Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*
...tively expressed FGFR-3, collagen type II, CD44, CD73, CD90, vimentin, Notch1, CD105, and CD106, but negatively expressed the haematopoietic stem cell surface marker CD34. Furthermore, CPSCs were successfully induced to differentiate into adipocytes, osteoblasts, and chondrocytes, showing that they have an in vitro differentiation ability. These findings provide a theoretical and experimental basis for cell transplantation therapy in tissue engineering....
Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5
... immunohistochemistry of collagen were performed. Results: Ivermectin was detected in 36 samples, out of them 15 contained high ivermectin levels (100 ppb). Chlorpyrifos, piperonyl butoxide, and acetamiprid were below the limit of quantification in 3 samples only. Histopathology of tick-infested skin revealed severe multifocal eosinophilic dermatitis and inflammation of subcutaneous hypodermis (panniculitis) whereas, in the skin with high ivermectin residue, t...
Eighty Mardiyan Kurniawati1*, Budi Santoso1, Fedik Abdul Rantam2, Widjiati3, Budi Iman Santoso4, Tri Hastono Setyo Hadi1, Gatut Hardianto1, Hari Paraton1
...e-epithelialization, and collagen deposition on day 21. Data were analyzed descriptively and statistically. Results: The mean scores of angiogenesis, fibroplasia, re-epithelialization, and collagen deposition in a New Zealand rabbit model of vesicovaginal fistula sutured with human amniotic stem cell seeding were higher than rabbits without amniotic stem cell seeding, the stem cell injection group, and the control group. The...
Salma Bessalah1, Samira Jebahi2, Asim Faraz3*, Amel Raoufi2, Cem Tırınk4, Walid Dridi2, Mahmoud Daouad2, Hassib Keskes5, Touhami Khorchani1, Khaled Farah2, Abdul Waheed3 and Mohamed Hammadi1
...that can be derived from collagen through hydrolysis. It most extensively used in diverse fields, mainly in pharmaceuticals and therapeutic. Herein Gelatin was newly extracted from camel (Camelus dromedarius) skin and irradiated with gamma rays from 60Co source. Gelatin was optimized and characterization and functional properties were determined. The structural changes occurring after γ-irradiation at doses from 5 to 30 kGy were reported by physico-chemi...
Nida Sadaqat1, Sheheyar Ahmed Khan1, Amna Bibi1, Sadaf Zahra1, Muhammad faisal Salamt1, Noreen Latief2 and Fatima Ali1*
... significant increase in collagen synthesis was observed in the NAC treated group with up-regulation of VEGF, IGF-1, PCNA, SDF-1 and CXCR-4 and down regulation of caspase3, P53 and CRP. The study showed that NAC is totally safe to use on rats. Hence for the future it is suggested that more experiments should be conducted for use in humans in conjugation medication for healing burn wounds....
Abdul Aziz1,2, Hamad Bin Rashid1*, Muhammad Arif Khan1, Asim Khalid Mahmood1, Ayesha Hassan1, Hamid Akbar1, Sadaf Imran1, Naveed Hussain1, Muhammad Umar1, Muhammad Asif1, Sajjad Javaid1 and Mamoona Chaudhry3
...cally digested with 0.1% collagenase-I. It was then centrifuged and the SVF pellet was collected. The pellet was characterized through total viable cell count, cell per gram and percentage of viability by using Neubauer’s chamber. The average cell yield per microliter was 2.992±1.527 x 104 cells/µl. SVF per gram of adipose tissues was 2.992±1.527 x 106 cells/g, percentage of viability was 97.98± 0.31%, while non-viable cell cou...
Mohammed A. Ali1*, Firas A. Alhasson1, Thaer A. Mohsin2
...inous cup contained in a collagenous matrix. The retina was avascular, and the layers of the retina were arranged in a normal vertebrate pattern. ...
Shirina Akter Toma1, Shah Ahmed Belal2, Md Abdul Baset3, Md Nazim Uddin3*
...oss was greater. Overall collagen content was highest in the thigh and breast muscles of the spent hen but the concentration of soluble collagen in broiler meat was greater. The spent hen was distinguished from broilers by having more moisture and ash contents (except wing meat of broiler), better pressing loss and Warner-Bratzler shear force value, and less intramuscular fat. Results revealed that broilers had the highest l...
Fen Wang1,2, Lin Zuo1,2, Xiang Zhou1,2, Zhu Chen1,2, Xiao Na Xu1,2, Suo Fei Ji1,2, Guan Jun Hou1,2, Cheng Jun Zhu2, Ye Zhang3, You Feng Su4, Gendong Jin5, Jia Jia Wang1,2, Yuan Gao6, Guang Tong Song1,2* and Ye Lin Jiang1,2*
...0.05). Additionally, the collagen content of type A calipash was significantly higher than that of type B (P<0.05). Collectively, our findings indicated that selective breeding could improve the nutritional value of T. sinensis from the Yangtze River....
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