Sogolo L. Lebelo1,* and Gerhard van der Horst2
...ant lipid inclusions and collagen fibers are also found in the cytoplasm of these cells. These cells are found associated with the spermatogonia type B, pachytene spermatocytes and Sertoli cells. The results from this study show that there are basic similarities in the Leydig cells of the vervet compared to other mammals, humans and nonhuman primates, however, there also seem to be species differences among the mammalian group. The association of these cells w...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...e HS, which can regulate collagen synthesis and deposition. To investigate collagen metabolism in keratinocytes and fibroblasts co-culture under pressure, and study the role of IL-1α and MMP-3 in terms of regulating fibroblasts-keratinocytes collagen metabolism with pressure.Chitosan-gelatin scaffolds were fabricated by vacuum freeze drying. Keratinocytes and fibroblasts were seedi...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...stepwise digestion by XI collagenase and trypsin was used to isolate MDSCs and were purified by differential attachment technique. Cell morphology were observed using an inverted microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDSCs were induced...
Kun Wang1,2, Yinglin Cui2, Xu Zhao2 and Changjiang Hu1*
...istration of stereotaxic collagenase type VII into the caudate nucleus. XN (at a high dose and a low dose of 3.60g·kg-1 and 1.80g·kg-1, respectively) was administered via enema. The detection of neuronal apoptosis was measured by TUNEL assay. Using immunofluorescence, the expression of claudin-5, ZO-1 and VE-cadherin was detected. The expression of DNA methyltransferase 3b (DNMT3b) and Matrix metalloproteinase-9 (MMP-9) was ...

 Li-na Li1, Sheng Li2, Ping Gui3, Jing-hui Li1, Fen Ai4,* and Li-li Cai1,*

...roliferation and reduced collagen deposition as measured by CCK-8 assay, Sircol assay, qPCR and western blot. MSCCM also reduced the expression of the pro-fibrotic TGF-β1 and increased the expression of anti-fibrotic IFN-γ at both mRNA and protein levels. Furthermore, we found that MSCCM inhibited the activation of NF-κB signaling pathway induced by irradiation as well. Our data suggest that MSCCM could reduce irradiation-induced TGF-β1 p...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
... proline residues in the collagen chain. The α subunit (P4Hα) of the P4H α2β2 tetramer contains the catalytic site, whereas the β subunit (PDI) maintains the solubility and activity of the α subunit. In this study, two plasmids were used to produce an active human P4H tetramer (rhP4H) in Pichia pastoris. The P4Hα was cloned into pPICZαA, and PDI was cloned into PHIL. Real-time PCR showed that the P4H gene wa...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF group and the control group with regard to heart rate, blood pressure, serum creatinine, BUN, LDH, cytokines and urinary electrolyte levels. Furthermore, monocyte and neutrophil apoptosis, tissue luminol, lucigenin, malondialdehyde and collagen
Arifa Savanur1,*, Tallat Naz1,2, Tayyaba Hamid1,3, Syed Abid Ali4, Mian Jahangir1,3 and Muhammad Abdul Azeem4
...s are less embedded in a collagen matrix. A significant difference between both types of muscle strips was observed in the rate of force re-development and change in their length. Thus, the present study provokes the need for quantitative histological and histo-chemical studies on the non-contractile proteins of Uromastix smooth muscle.

Rafiullah Khan1, Waseem ur Rahman1, Misbah Ullah2, Kamran Afaq3, Muhammad Amjad1, Sakhi jan1 

...s matrix binder and soft collagen as reinforcing fibers. As bone supports all the organs of a body so its
mechanical behavior is of prime importance. The mechanical properties of bone varies with age. In this paper, the
effect of age on various mechanical properties of hip joint bone was investigated experimentally. For this purpose
hip joint bone specimens of male bovine of different age groups were tested for fracture toughness, young mo...

Olga Mikhailovna Blinnikova1*, Vadim Anatolyevich Babushkin1, Lyudmila Gennadievna Eliseeva2 and Galina Severyanovna Usova3

Modeling a Formulation and Assessment of the Consumer Properties of the Special Purpose Starch Drink
...sing of a hydrolyzate of collagen as a component of the enriched foodstuff opens unlimited opportunities of production of the goods for functional, medical preventive and medical purposes, capable to fill the shortage of collagen and to stimulate the human body to produce its own protein. Drinking kissel is a perspective product type for enrichment the CCR (Central Chernosem Region’s) fruit and berry’s by
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
Qiang Tang1 and Qianli Tang2*
..., and width of subdermal collagen fibers also decreased (p<0.001), inflammatory factors TNF-α, IL-6 and IL-1β in skin tissue increased (all p<0.001), and expression levels of Ang-1 and Tie-2 proteins in skin tissue decreased (both p<0.001). Compared with model group, thickness of epidermis, dermis and collagen fibers, and expression levels of Ang-1 and Tie-2 proteins in skin tissue in...
Imran Tarique1*, Muhammad Ghiasuddin Shah1, Illahi Bux Kalhoro1
Zaheer Ahmed Nizamani2, Mansoor Tariq 2, Jamil Ahmed Gandahi1
Saqib Ali Fazlani3 and Benazir Sahito3
...hich consist of elastic, collagen and reticular fibers. Superficial dermal papillary layer was located beneath the epidermis, whereas dermal reticular layer deeper above the hypodermis. The papillary and reticular dermis was well developed in adult male age group with abundant areolar and dense irregular connective tissue all skin regions. Skin appendages (sebaceous and sweat gland, and hair follicle) was well developed and distributed in papillary and ...
Zheng Yan1,2,*, Shuang Liu2, Ai-Qing Liu2 and Hai-Yan Wang2
...traions of three type of collagens, hyaluronic acid and hydroxyproline in skin tissue were also determined. The results indicated that the oral administration of elastin peptide greatly improved the skin elasticity, accompanying with significantly upregulated expression of hyaluronic acid and hydroxyproline. In addition, the contents of collagen in animal skin were also significantly increased, especially Type III and IV

Pakistan Journal of Zoology


Vol. 54, Iss. 1, Pages 1-501


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