Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
... on the short arm of the chromosome number 6 and position 21 and the allele frequency is associated with allergy. The study was conducted to investigate the relationship between the aero-allergens and the frequency of HLA-DRB1*11 and HLA-DRB1*12 allele in aeroallergen sensitized individuals. Blood samples of aeroallergen sensitized individuals were collected at the Allergy Centre, NIH, Islamabad. Then samples were analysed at the Department of Mi...
Ümit Kumbıçak, Zübeyde Kumbıçak* and Gülnare Huseynli
...tures, mitotic metaphase chromosomes, karyogram and course of meiosis of Hersiliola bayrami were determined based on Giemsa-stained testicular chromosomes for the first time. Results of spermatogonial metaphases showed that the chromosome number and the karyotype formula of the species was 2n♂=35 (X1X2X30) which is the first record for the fami...
Syed Kashif Nawaz*, Faiza Zubair and Sidra Kanwal
...ng genes were present on chromosome 1. Chromosome 21 carried the minimum number (85) of overlapping genes. Overlapping genes were further categorized on the basis of their orientation. It was found that the human genome has 62% embedded genes, 17% convergent genes, 16% divergent genes and 5% uni-directional genes. These categories may further be differentiated as coding vs. coding, coding vs. non-coding and mixture of both t...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...alysis out and find that chromosome is 2n=78 and shows normal. CAFSCs in mid period of subculture propagate fastest from cell cycle examination on Flow Cytometer and growth curve using CCK-8 kit on ELIASA. CAFSCs are induced to neuron and epithelium (epiblast), adipocyte (mesoblast), hepatocyte (entoblast).
...
Saba Irshad*, Aruba Muhammad, Ammara Muazzam, Farah Sarfraz Anmol and Rehman Shahzad
...the Gamma globin gene on chromosome 11 of positive patients (GenBank KY927385). Fetal hemoglobin level in Xmn1 (-/+) and (+/+) was 59.1% and 19% respectively which minimize their transfusion frequency to 30 days in comparison to 7-15 days in Xmn1 -/- patients. Hematological analysis of thalassemic patients revealed low Hb, WBCs and platelets counts in contrast to control. The reported polymorphism was meant to be lowering the frequency of blood transfusions an...
Syeda Ain-ul-Batool1, Sadia1, Kathrin Blasius2,3,4, Angela Kaindl2,3,4 and Ghazanfar Ali1,*
... was detected in BBS9 on chromosome 7p14.3 using whole exome sequencing (WES). A splice acceptor site mutation (c.1131G>A) in exon 3 was revealed by Sanger sequencing.
... 
Tahira Jatt1, Ghulam Sarwar Markhand1, Lane Rayburn2, Ray Ming3, Mushtaque Ahmed Jatoi1 and Ameer Ahmed Mirbahar1,4*
...ions were carried on the chromosome number and karyotype one of the seven indigenous commercial date palm varieties and four wild type date palm of Sindh province, Pakistan by using the traditional Fuelgen squash method. Results indicated that all seven commercial varieties and four wild types of date palm are diploid in nature and having 2n=36 chromosomes. First time detailed karyotype of these varieties have been developed...
Zübeyde Kumbıçak1,* and Hatice Poyraz2
...rs of autosomes plus sex chromosomes which were X1X20 (♂) type. All chromosomes including sex chromosomes were telocentric and relative lengths of autosomal pairs were decreased gradually in size. All male meiosis was chiasmate and during the first meiotic division stages, 10 autosomal bivalents and generally one chiasma per bivalent (rarely two chiasmata) were obta...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1
...aphical distribution and chromosome number. In Pakistan the Chukar partridge (A.chukar)), is an important member of Phasianidae family, however a scarce molecular data is reported that urged us to investigate its genetic diversity and phylogeny using mitochondrial DNA, Cyt-b and Cox1 genes. A total of 749bp of Cox1 and 472 bp of Cyt-b complete coding regions of both genes were amplified by PCR followed by sequencing. The sequ...
Guofang Wu1,2, Xingxing Xue1, Wenjuan Shen1, Lei Wang1,2*, Yuhong Ma1 and Jiping Zhou1
... 2 gene (RAI2) on chromosome X in Sus scrofa (SSCX). Analysis further revealed that the L2-(AC)n locus, featuring AC repeats, and the L3-(TC)n locus, featuring TC repeats, were located in intergenic regions on SSC14 and SSC5, respectively. TGA repeats within the L4-(TGA)n locus were located in the inner mitochondrial membrane peptidase subunit 2 gene (IMMPL2) on SSC18. Further association analysis revealed ...
Halil Erhan Eroglu
...esigned by the number of chromosome types is S/AI = (1 × M) + (2 × SM) + (3 × A) + (4 × T) / 2n. The S/AI value varies from 1.0000 (full symmetric) to 4.0000 (full asymmetric). After a detailed literature review, the chromosomal data of 36 female species and 32 male species of family Cervidae were detected, namely (i) karyotype formulae, (ii) symmetry/asymmetry index values (iii) karyotype types. According to the chromosomal data, two p...
Maria Qibtia1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Sehrish Faryal1, Ahmed Mansouri2, Zeeshan Ahmad2, Muhammad Hamid3 and Ali Raza Awan1,*
...>LCT-gene located on chromosome 2q21. In this study, 50 patients with LI were investigated for the identification of mutations in the LCT-gene. In comparison of 30 subject with lactose persistence (LP) considered as healthy group. A total of 13 genomic mutations were identified, 1 in the promoter region and 12 in the intronic/exonic region of LCT-gene. Among these 12 mutations, 6 of them are novel in origin. The novel mutations were found in ...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...n SRY gene, located on Y chromosome. Hence, the presence of heavy metals, even in small quantities in the bodies of oligozoospermia males can be one of the main causative agents of male infertility.
...
Hadia Gul1, Abdul Haleem Shah1, Ricardo Harripaul2, Anna Mikhailov2, Ejaz Ullah Khan3, Wasim Shah3, Nisar Ahmad3, John B Vincent2,4 and Muzammil Ahmad Khan3*
...d a 1.2 Mb HBD region on chromosome 15 between markers rs4778147 to rs8036234 (chr15:27752745-28962131bp), which harbors the previously reported OCA2 gene. The subsequent whole exome sequencing identified a novel splice site defect at spice donor site, wherein G was replaced by T at genomic position 28171268 (NM_000275.2:c.2079+5G>T) within intron-19 of the OCA2 gene. This change presumably may either includes few intronic nucleotides or skip exon-20 in the...
Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra
...lar to Bos mutus (yakQH1 chromosome 19) and did not align with reported Bubalus bubalis GH sequence. Only 49% of the sequenced product aligns with Bubalus Bubalis though 90% of the sequence aligns with Bos mutus and Bos indicus GH gene. A 46 bp inverted repeat sequence was also identified upstream exon 1. This report not only raises questions about the purity of the gene but also indicates mutations which may affect animal productivity like milk yield, growth ...
Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella
...the CD18 gene segment on chromosome 1 exon 2 with a target length of 106bp has been successfully carried out with a success rate of 100%. RFLP analysis of NCOI cutting restriction enzymes shows that there are two bands at 66bp and 40bp. This shows that the normal allele frequency is one and the mutant allele is 0 with a heterozygous genotype frequency of 0% in the population. According to the result, the polymorphism that appears in the CD18 gene by PCR-RFLP m...
Roshana Mukhtar1, Shaheen Shahzad1*, Sajid Rashid2, Maryam Rozi2, Madiha Rasheed3, Imran Afzal4 and Pakeeza Arzoo Shaiq5
...uence variant of UROS on chromosome 10q252-q263. The enzyme catalyzes the fourth step of heme synthesis pathway. The present study focused on the clinical assessment of CEP affected individuals in a Pakistani consanguineous family by Sanger sequencing of UROS gene to identify potential pathogenic sequence variants. CEP patients were identified using successive clinical tests. Blood samples of patients were collected and processed for genomic DNA extraction fol...
Ahlam G. Khalifa1*, Walaa A. Moselhy1, Hanaa M. Mohammed2 and Khaled Abdou1
...significant elevation in chromosome aberrations. Also, the ratio of polychromatic erythrocytes to normochromatic erythrocytes proved that deltamethrin had a stronger cytotoxic effect on the bone marrow cells than the CS/DM Nps and S/DM Nps groups. It was concluded that these innovative nanoformulations of DM were less toxic, which would lower health risks and boost application safety.
Keywords | Cytotoxic, Chromosome...
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
...racteristics of X- and Y-chromosome-bearing sperms separated by using albumin gradient and swim-up methods in Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome
Ali I. Al-Ameedi1, Zahraa M. Ayad2*, Wed Abbas Mohammed3, Salim K. Hajwal4
...ations such as dicentric chromosome, Acentric, deletion, ring, and a high rate of breaks in bone marrow cells. As a results, GbE would be good candidates for administration as a supplement to decrease the Geno-toxicity side effects of a lot of chemotherapeutic agents.
Keywords | Ginkgo Biloba, Genotoxicity, Hyroxyurea, Protective effect, Phytochemical
...
Fang Chen
...nsin homology deleted on chromosome 10 (PTEN) on the drug resistance of small cell lung cancer (SCLC) to apatinib. Human SCLC cell line H69 was purchased from the American Type Culture Collection. Through stepwise screening, an apatinib-resistant SCLC cell line (H69/Apatinib) was established. All cell lines were stored in RPMI-1640 medium, which was supplemented with 10% fetal bovine serum, 100 µg/ml streptomycin and 100 U/ml penicillin and kept in humid...
Muhammad Salman Ikram1, Tahir Mehmood1,2*, Sehrish Firyal3, Huma Sattar4, Shagufta Saeed3, Fareeha Nadeem3 and Muhammad Imran Mahmood Khan5
...orensic science, Human Y-chromosome markers are extensively used for DNA analysis of such cases with limited information of autosomal DNA profiling, difficult to obtain data or identification has to be made from Y-chromosome markers. This review describes the uniqueness of structure and relative position of Y-STRs loci, history of Y- chromosome, role of genetic markers like Y- Short tandem...
Baoxuan Nong1,2, Biqiu Wu3, Anlong Xu2, Wenai He2 and Yongfu Qiu2*
...677. Wbph1 was mapped on chromosome 2 of N22 in a region that harbored the markers RM13650 and RM13478. Its largest logarithm of the odds (LOD) score was 3.94, which explained a 16.6% of the phenotypic variation. Wbph9 was mapped on chromosome 3 of OB677, where it was flanked by markers RM3513 and RM3525. It had a LOD score of 3.4, explaining a 17.2% of the phenotypic variation. Four varieties PTB33, N22, RBPH327, and OB677 ...
Budi Utomo1*, Amaq Fadholly2,3, Endang Tri Margawati2, Alek Ibrahim2, Ristaqul Husna Belgania1, Muhammad Fajar Amrullah1, Rimayanti1 and Hermin Ratnani1
... the paternal pathway (Y chromosome). The DNA samples used for this study were 10 frozen straw of Limousin cattle and 10 samples of Madrasin cattle. The method used in this research was a descriptive analysis by duplex polymerase chain reaction and analyzing the results of the SRY gene sequencing of limousine cattle and Madrasin cattle. In the present study, we showed that the SRY gene of Madrasin cattle and Limousine cattle could be amplified with SRY F and S...
Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee
