Hu Zenglei1 and Xiufan Liu1, 2*

 

Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2 

...n breeds. A total of 800 chickens (400 Huangshan black chickens and 400 Huainan partridge chickens) of similar age and weight were evaluated in the present study. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible association with egg production performances including age at first egg (AFE), laying rate (LR), egg production (EP), egg weight (EW)...

Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns

 

...ressive disease in young chickens. The first line of defence against the virus is innate immunity whose activation is independent of the antigen and depends on the ability of the host to recognize pathogens by means of specific pattern recognition receptors. This interaction induces the production of cytokines, chemokines and interferons. Through Janus kinase/signal transducers and transcription activators pathway, interferon induces the transcription of inter...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2
...ant neoplasm in domestic chickens with subsequent economic losses to poultry industry. Effected birds show lymphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked ...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...ular immunization of SPF chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were...
Vladimir Avdalovic1, Marijana Vucinic2, Radmila Resanovic3, Jelena Avdalovic4, Danka Maslic-Strizak5 and Milos Vucicevic3*
...matitis (FPD) in broiler chickens. The study included 300 one-day-old Ross 308 broilers. One group of broilers was reared on chopped wheat straw (the particle length of straw ≥ 10 cm). The second group was kept on newly formulated pelleted wheat straw. The duration of the trial was 42 days. Moisture content in litter materials and FPD occurrence and severity were assessed weekly. First signs of FPD were observed in 21-day old chicke...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...ific pathogen free (SPF) chickens with two different concentrations of Egy-H5 plasmid DNA 15µg and 60 µg. Results demonstrate that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vac...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
...he increasing demand the chickens are reared commercially through feeds and battery cages allowing maximum weight gain and growth in considerably less time. The constituents that are fed to chickens concentrate in the flesh and bring deleterious health outcomes on the consumers. The ill effects account for hyper-lipidemias and imbalance in the steroidal sex hormones may result in the development of cysts in the ovaries with ...
Alaeldein M. Abudabos*, Abdullah N. Al-Owaimer, Elsayed O.S. Hussein and Mutahar H. Ali
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...hering Huainan partridge chickens. A total number of 360 fast feathering Huainan partridge hens and 40 roosters were randomly divided into two equal groups. For NM, 4 mating cages were maintained each with 5 roosters and 45 laying hens. The other birds were reared in 3-layer complete ladder cages for AI, for which 180 hens were maintained in 60 cages with 3 hens/cage and 20 roosters in 20 cages. Laying performances, mortality, egg quality characteristics and w...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

...isolates from humans and chickens. Our results revealed that linking the epidemiological and sequence data is important to understand the prevalence, transmission, persistence and evolution of the virus, and to monitor the circulating AIV strains and emergence of new AIV subtypes in Egypt. The wide circulation of the 2.2.1.2 subclade, carrying triple mutations (120, 129∆, I151T) associated with increased binding affinity to human receptors, is an alarmin...

Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1* 

...nteritis (NE) in broiler chickens. The influence of varying concentrations of both EOs was determined in vitro using the agar dilution test. Concentration as low as 0.25% and 0.125% of thyme and clove oils, respectively, were able to completely inhibit the growth of CP. An in vivo study was conducted in commercial broiler chickens, where, 2 groups of 10 day-old broilers were fed a balanced ration with addition of thyme (0.25...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,* 
...contents in TD afflicted chickens compared to control group. The SM administration to TD affected birds significantly ameliorated lameness, stimulated ALP level with a decrease in ALT and AST contents, increase in antioxidant parameter and decrease in MDA contents significantly (P<0.05). SM treatment of TD-afflicted birds prevented lameness and reinstated antioxidant imbalance. SM may be effective for the treatment and control of TD and minimizing the liver...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ardium syndrome (HPS) in chickens worldwide. Identification of fowl adenovirus (FAdV) serotype is of most importance in epidemiological studies of disease outbreak and the adaptation of vaccine strategies. In spite of appropriate administration of vaccination, FAdV outbreaks have been reported and caused significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and gain a better understanding of the genetic properties o...
Ali Karabayir1,*, Nazan Arifoğlu2 and Mustafa Öğütcü3
...(local mix-breed village chickens) was evaluated in the present study. Supplementation of disinfectant clearly improved the microbiological quality of chicken drinking water, by reducing the total bacteria, coliform and E.coli counts. On the other hand, disinfectant addition decreased feed and water intake of all chicken strains. Furthermore, the results revealed that feed and water intake was strain dependent. Lower feed conversion ratio was observed f...

Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq

...es(%) values of negative chickens were 9.43±0.49 g/dl,, 25.81±1.34%, 1.84±0.13 x106/µL, 151.7±11.0 fl, 3.85±0.22 pg, 39.34±2.07%, 3.98±0.13 x103/µL, 45.71±1.26%, 2.08±0.29%, 1.54±0.17%, 2.58±0.29% and 48.10±1.36% and for positive chickens were 9.35±0.89 g/dl, 24.63±2.21%, 1.68±0.17 x106/µL, 168.9±...
Kalimullah Khan1,*, Hafsa Zaneb2, Zia Ur Rehman3, Hamza Maris4 and Habib ur Rehman1

Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1 

...n but also it can infect chickens and its virulence enhanced upon passaging in chickens causing clinical signs and deaths. In the present study brain samples were collected from twelve pigeon flocks exhibiting signs of nervousness and diarrhea in a live bird market (LVM) in Egypt. All collected samples were positive for NDV and negative for avian influenza virus and infectious bronchitis virus. Seven positive samples were su...
Ali Raza Jahejo1,2, Nasir Rajput2, Wen-xia Tian1,*, Muhammad Naeem2, Dildar Hussain Kalhoro2, Asmatullah Kaka2, Sheng Niu1 and Fa-jie Jia1
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
... Lohman Brown genus 8000 chickens. According to the findings of the analysis, it was seen that January, March, October, November and December are the months when production performance is high. When these months were ranked among themselves, it was observed that January, March and November are the first three ranks, respectively, and that the rank was also supported by the combined results of different techniques. As a result, in terms of production performanc...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...3 Japanase quails and 22 chickens were analyzed by conventional and molecular methods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by d...
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
...ondroplasia (TD) and the chickens were injected with different doses of recombinant glutathione-S-transferase A3 (rGSTA3) proteins. We explored the responses of HDPs in erythrocytes to thiram-induced TD and rGSTA3 protein by quantitative real time PCR (qRT-PCR). The results showed many HDPs expressions were suppressed by thiram-induced TD, and the expressions of HDPs were upregulated by rGSTA3 protein. These findings demonstrated that mRNA expressions of HDPs ...
Chen-Yu Sun, Wen-Chao Liu*, Mei Xiao, Zhi-Hui Zhao and Li-Long An*
...ale, one-d-old Huaixiang chickens with an average initial BW of 44.52 ± 1.07 g were randomly allotted to 4 treatments. Each dietary treatment consisted of 6 replicate cages, with 15 birds per replicate. The 4 dietary treatments were corn-soybean meal-based diets and supplemented with 0, 500, 1000 and 2000 mg/kg betaine, respectively. During d 1-14, with increasing the dietary betaine levels from 0 to 2000 mg/kg, the BWG was improved (quadratic, P<0.0...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1Muhammad Faheem Malik3 and Muhammad Idrees4

 

...megaly syndrome (HSS) in chickens. To the best of our knowledge the circulation of aHEV in chicken has not been studied in Pakistan so far. Therefore, in the present study, we aimed to isolate and identify aHEV from layer chickens of Pakistan. The bile fluids, liver and spleen tissues were collected from overnight dead layer chickens (n = 8) from Pattoki region of Punjab province, Pakistan...
Zia-ud-Din1, Muhammad Nauman Aftab2, Tanveer Hussain3,*, Irfana Iqbal4 and Asma Zafar5
...rieties (P<0.05). The chickens given feed with probiotic (@1g/kg of feed were of more economic value than those fed on antibiotic supplemented feed and control with highest live body weight of 183.58±6.31g gaining 143.13±6.37g weight, multiplying their weight 4.56±0.17 times over the initial body weight with feed intake of 668.82±31.11g showing most efficient FCR (4.67±0.25) with least mortality and highest net profit (Rs....
Hande Kucukonder1, Pinar Celebi Demirarslan1*, Sezai Alkan2 and Özgür Baris Bırgul3
...broiler growth curves of chickens with nonlinear regression analysis and grey prediction model. For this, the growth of 118 broilers was analyzed by using their weekly individual live weights from hatch to 49 day-old. In the analysis, nonlinear functions and Rolling-Grey Model (1,1) prediction method were used. The time-dependent growths of mixed sexes broilers were analyzed in the aspects of testing the parallelism of female and male growth samples, determini...

Shwe Yee Win1, Lat Lat Htun1, Myint Myint Hmoon1, Hla Myet Chel1, Yu Nandi Thaw1, Nyein Chan Soe1, Htet Lin Oo3 and Saw Bawm2* 

...nal parasites in village chickens from four Townships (Mawlamyine, Nyaung U, Zay Yar Thi Ri and Katha) of Myanmar from April to June 2019. A total of 200 faecal samples were randomly collected from village chickens. Centrifugal sugar floatation and sedimentation methods were carried out to detect the gastrointestinal parasites. The overall occurrence of gastrointestinal parasites in village chickens<...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
.../i>Pullorum infection in chickens. However, whether the 3’-untranslated region (3’-UTR) of the MyD88 gene is associated with resistance to Salmonella Pullorum infection still remains unknown. In this study, a total of eight SNPs, including three novel mutations [SNP4 (A4812316G), SNP6 (C4813363A) and SNP7 (C4813618T)] and five known loci, were found within 3292 bp sequenced fragments. The allele frequency and genotype frequency of SNP...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool4, Laraib Liaquat3, Fatima Ahmed5, Asra Khan1, Tahira Perveen3, Mirza Jawad ul Hasnain6, Saima Khaliq7 and Saad Bilal Ahmed8
...n of commercial feed for chickens and their meat which is considered safe for human consumption.
...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...ontact with the examined chickens. Eleven Campylobacter isolates were recovered through bacteriological examination. Campylobacter spp. were identified by polymerase chain reaction (PCR) as C. jejuni (63.6 %) and C. coli (36.4 %) through the detection of the Map A and Ceu E genes, respectively. The antibiotic resistance of the Campylobacter isolates was determined via the disc diffusion method and was observed m...
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
...ne competence of broiler chickens. In three experiments (n=240 chicks in each), the immune modulators vitamin C (500 mg/l), vitamin E (200 mg/l), dietary nucleotides (100 mg/l) and DNA (100 mg/l) extracted from chicken liver were separately offered on days 1-21, 1-42 and 22-42 in experiments 1, 2 and 3, respectively. Under standard broiler management conditions, birds were divided into five treatment groups of 48 with four replicates. Commercial pre-starter fe...
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...ine efficacy, commercial chickens were vaccinated with La Sota (against NDV) and Massachusetts (against IBV) vaccine preparations. After screening the protective titers in birds at 19 days post inoculation three week-old broilers were challenged with 10-5.76 and 10-6.03 EID50 dose of NDV and IBV, respectively. The hosts were divided into six groups of 35 birds each. These were groups A (NDV-challenged), B (IBV- challenged), C (...
Yan Zhou1,2, Hai Xia Han1,2, Qiu Xia Lei1,2, Jin Bo Gao1,2, Wei Liu1,2, Fu Wei Li1,2, Jie Liu1,2 and Ding Guo Cao1,2*
...VLDLR on reproduction in chickens, the haplotypes and diplotypes based on three genetic mutations (NC_006127.2:g.8467G>A, NC_006127.2:g.12321G>A and NC_006127.2:g.13876A>G) were constructed, and the associations of diplotypes with reproduction traits were assessed, their effects on gene expression were evaluated also. As a result, three haplotypes H1 (G-G-A), H2 (G-G-G) and H3 (A-A-G) were obtained, H1 was the main haplotype with a frequency of 91.75%...

Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*

Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...in unimmunized humans or chickens. In reducing losses due to H9N2 infection, rapid diagnosis is an option to prevent further infections. Office International des Epizooties (OIE) suggested some methods for the detection of H9N2 infection including the immunofluorescence method that is commonly known as the direct fluorescent antibody (DFA) assay. This method is a combination of immunology and bio-imaging. This DFA assay is rapid and sensitive, but specificity ...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
...with VVND virus infected chickens. Clinical signs were observed in Japanese quails and mynas on 4th day post-inoculation. The major clinical signs were green yellowish diarrhea, ruffled feathers, anorexia and torticollis in all groups except Q3. The mean clinical scores and mean death time (days) were statistically non-significant (P>0.05) among all the groups. Higher mortality rate (75%) was recorded in mynas as compared to Japanese quails (50%)...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6 

Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3

...alo, sheep, goats, pigs, chickens, geese, turkeys, ducks, guinea fowls, dogs and bees were analyzed. The number of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic resources, be included in the Decree in the future, in order to preserve and improve their numbers. An urgent establish...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Ying Wang1, Ding Zhang1, Yu-hai Bi1,2, Ali Raza Jahejo1,Qian-qian Cheng1 and Wen-xia Tian1,*
...rocytes of H9N2 infected chickens. In current study, qRT-PCR and Western blot analysis were performed to identify the expression levels of PG-related genes (COX-2, GSTA3, PTGDS, PTGES, PTGER3 and PTGER4) in erythrocytes of H9N2 infected chickens at time intervals of 0, 2, 6 and 10 h and P < 0.05 was considered as statistically significant. Expression levels were increased significantly in case of COX-2 at 0 and 6 h...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
...e performance of broiler chickens. The study used 120 unsexed Hubbard one day old broiler chicks randomly divided into three groups of 40 chicks that were each further divided into four replicates of ten chicks. Extracts were introduced at 200 mg/Kg as feed supplement to two groups of broilers and the third group was kept as control (C) and offered a basal diet. The chicks were reared in floor pens bedded with saw dust litter. Measured feed was offered twice a...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...AI viruses isolated from chickens, Egypt during 2009-2011. Both inactivated and recombinant fowlpox virus expressing H5 (rFP-AI-H5) vaccines were measured. Vaccine efficacy was evaluated by percentage of protection from mortality, morbidity and reduction in virus shedding from respiratory and/orintestinal tracts. Despite the H5 antibody responses in vaccinated chickens with program I (rFP-AI-H5" vaccine then Re-5) being...

Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***

...s bronchitis diseases in chickens.

...

Amir M. Walaa1 ; Ausama Yousif1; Walid H. Kilany2; Magdy El-Sayed1; Ahmed A. El-Sanousi1

...ect experimental broiler chickens against challenge with wild ND and IB viruses. Two hundred and eighty commercial one-day-old broiler saso chicks were divided into 8 groups (35 birds each). Groups 2, 3, 4&5 were vaccinated at one-day-old with live hitchner + IB Primer. Then all groups at 7 days old were boosted with inactivated combined NDV+IBV vaccines with different adjuvants except for group 1 & 2. Group 1 was kept as unvaccinated control. At 28 da...

Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar

...of nine week old Hubbard chickens were vaccinated with two different commercial live attenuated Chicken anemia virus (CAV) vaccines. One was vaccinated with 26 P4 strain vaccine subcutaneously and the other was vaccinated with Cux strain vaccine through drinking water (DW). Then the inactivated avian influenza (AI) H5N1 vaccine was inoculated intra-muscularly either at the 1st, 2nd or 3rd WPV with CAV vaccine forming three subgroups per each group. It is clear...

Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2

...ruses were isolated from chickens and ducks in both live bird markets and backyard populations. NS1 gene was amplified, sequenced and the phylogenetic analysis of the obtained sequences revealed deletion of 5 amino acids at positions 80 to 84 in the NS1 protein along all Egyptian isolates under study. Ala149, which is a pathogeneicity marker for interferon antagonism was found in the studied indicating capability of these viruses to transmit between different ...

Serageldeen Sultan1, Nabila Osman2

...) and commercial broiler chickens (n=84) from different farm flocks (24 flocks) in Egypt. These samples were examined for the presence of avian HEV antibody using enzyme linked immuno-sorbent assay (ELISA). The results revealed that the occurrence of avian HEV was 15.7% (80/510) and 66.7% (16/24) of the examined flocks. HEV antibody prevalence increased as age dependent and most commonly occurred among broiler breeder flocks (10/24). In conclusion, the occurre...

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

... from 14-day-old broiler chickens with proventriculitis in Qaluobia province of Egypt in 2014. Objective: The isolate was identified by a real time RT-PCR targeting the nucleocapsid (N) gene with further genetic and antigenic characterization. Methods: Beside a real time RT-PCR; sequence analysis of partial S1 gene, cross-neutralization and the challenge experiment for H120 vaccinated broiler chickens were also performed. Re...

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

... occurring in vaccinated chickens with great economic losses. Objective: This work aimed for diagnosis and characterization of Newcastle Disease Virus (NDV) from infected broiler chickens in Egypt. Methods: Suspected virus isolates on embryonated chicken eggs was detected by Hemagglutination (HA) test and identified using hemagglutination inhibition (HI) test. F protein encoding gene of NDV was amplified using Reverse Transc...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...00%, 60% and 60 % of the chickens in tracheal swabs
and 100%, 80% and 60%in cloacal swabs when tested by virus isolation in eggs at 3, 5 and 7 days post
challenge; respectively. Swabs of 10 days post challenge of group 3 were positive by rRT-PCR and
negative by virus isolation. On the other hand, group 6 (vaccinated with imported vaccine) demonstrated
shedding % at 3 and 5 days post challenge by both rt-RT-PCR an...

Shimaa M. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A. Eid

...rus infection in broiler chickens with
availability of Vertical Transmission
Methods: Herein, 18 chickens derived from 4 broiler flocks within Sharkia Province, Egypt suffering
from different degrees of lameness and/or stunting were clinically and molecularly examined for the
presence of ARV.
Results: By clinical examination, one third (6/18) of examined
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
... and 80% and 20 % of the chickens in tracheal swabs and 80% and 40% in
cloacal swabs when tested by virus isolation in eggs at 3 and 5 days post challenge; respectively. Whereas
swabs of 7 and 10 days post challenge of group 3 were negative by rRT-PCR and virus isolation. On the
other hand, group 6 (vaccinated with imported 500HAU vaccine) demonstrated shedding % at 3 and 5 days
post challenge by rRT-PCR and for ...

Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2

...challenge pathway of SPF chickens
to estimate the protection percent of each vaccine against both classical and variant HPAI viruses.
While, the invitro method was done by the direct detection of the viral content and viral identity using
the rRT-PCR technique from final product of these inactivated vaccines.
Results: The data of this study showed that sufficient HA antigen content and similar HA sequences
...

Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2

...mmune response of turkey chickens.
Methods: Four groups of bronzy turkey chickens were used in this study; group-1 received live
attenuated TRT vaccine, group-2 received inactivated AI vaccine, group-3 received TRT and AI
vaccines and group-4 served as non-vaccinated control. The humoral immune response evaluated
serologically by using hemagglutination inhibition test (HI), ser...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi
...the respiratory tract of chickens. In
Lebanon; there is a few data concerning the IBV among poultry flocks.
Objective: The aim of the present study is to detect the molecular characterize IBV in samples
collected from Lebanese flocks geographically distributed in 7 Governorates.
Methods: Blood sera, tracheal swabs and tissue organs were collected from 60 poultry flocks from
different ages, different t...

Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi

...N2 in different types of chickens including broilers,
breeders, laying hens, backyard chickens and ducks. Tracheal swabs were collected from flocks raised
in different governorates in Lebanon.
Results:Testing of samples with rt-RT-PCR revealed that 6 flocks reared in 4 Lebanese governorates
were positive (CT ranged from 20.42 to 33.86). A Positive sample with CT 20.42 was teste...

Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon

...stable ND vaccination in chickens. Sera obtained from forty (40) chickens per LGA during
pre-vaccination, post-vaccination and post-booster vaccination with NDV4 thermostable vaccine were
subjected to Hemagglutination Inhibition (HI) test.
Result: Antibody detection showed seroconversion in chickens 28day post vaccination and post
booster vac...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
... programs against NDV in chickens,
one using genotype VII inactivated and live NDV vaccine are available in the market and
second group used genotype II inactivated LaSota vaccine with live vaccine.
Method: NDV isolate designated NDV-GZ-986F-2015 was isolated from a commercial
broiler farm in Egypt and characterized as a virulent strain was used for challenge. Contact
chicks were added in both groups ...

Ahmed Ali Moryani1, Nasir Rajput1*, Muhammad Naeem1, Atta Hussain Shah1 and Hidayatullah Soomro2

...e performance of broiler chickens challenged with coccidiosis. A total of 450 broiler chickens were allotted into 6 groups (n = 75/group). Group A controlled without supplementation, Group B, C and D were supplemented with Aloe barbadensis (5ml/L), Ferulafoetida regal (500mg/L), Tamarindus indica (50mg/L), respectively. Group E and F were supplemented with a mixture of all 3 herbs/plants at 2ml/L; mixed with distilled water ...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1,  Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...’s disease (MD) in chickens. Chicken erythrocytes are nucleated cells of myeloid origin which contribute to gases exchange, transportation and immunity. The objective of this study was to reveal the immune responses of MDV-infected erythrocytes of chicken by transcriptome analysis. Twenty specific pathogen-free (SPF) chickens were divided into two groups as control and treatment. The chickens
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...asitic disease affecting chickens. In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. Haematological examination of ...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*
...ajor cause of illness in chickens with potential threats to human health. Chicken erythrocytes are known as most abundant circulating cells in the body which participate in inflammation and immune responses. Influenza viruses interact with the host cells at cell surface, leading to cell death in human and animals through apoptosis. Current study was designed to investigate the expression levels of apoptosis-related genes in the H9N2 infected chicken erythrocyt...
Jia Liu1, Zhen Wang1, Zifan Ning1, Ali Mujtaba Shah2,3,Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Yaofu Tian1, Diyan Li1,Gang Shu2,4, Lin Ye1 and Xiaoling Zhao1,*
..., and slow- growing (SG) chickens, however, the underlying molecular mechanisms responsible for the differences in post-hatch muscle development are unclear. Here, we report on the identification of several candidate genes that may modulate myofiber growth and thus explain some of the differences in the skeletal muscle phenotype of FG and SG chickens. We collected pectoralis major (PM) and gastrocnemius muscles (GM) on d 1, ...
Ziyue Qin1, Zhen Wang1, Ali Mujtaba Shah3,4, Zifan Ning1, Yaofu Tian1, Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Lin Ye1, Diyan Li1, Gang Shu2 and Xiaoling Zhao1,*
...rs. However, whether the chickens with different genetic background have the same response to feed restriction is not reported, herein we evaluated three levels of feed restriction effects on growth performance and blood glucose concentrations in faster- and slower- growing chicken (different genotypes). In the present study, birds were restricted to 100% to full feed in faster- and slower- growing broilers groups. Body weight gain, feed consumption, blood glu...
Khanitta Ruangwittayanusorn1, Doungnapa Promket1*, Kamonnate Pimrueng1, Jennarong Kammongkun2
... Pradu Hangdum Chiangmai chickens (HPHC). PCR-RFLP protocols were used to identify polymorphisms of both genes. The frequencies of TT, TC and CC genotypes of DRD2 were 0.05, 0.40, and 0.55, respectively. The frequencies of T and C alleles of DRD2 were 0.25 and 0.75, respectively. Regarding VIP, the frequencies of II, ID and DD genotypes were 0.59, 0.28, and 0.13, respectively. The frequencies of I and D alleles of VIP were 0.73 and ...

Ahmed M. Elbaz*, Engy F. Zaki, Morsy A. S. 

... meat quality in broiler chickens. A total of four hundred and eighty 1-day-old Ross 308 chicks were randomly divided into four experimental groups: Control (CON), 5% of quinoa seeds (QS5), 10% of quinoa seeds (QS10), and 15% of quinoa seeds (QS15) groups. Results indicated that increasing inclusion levels of QS improved body weight gain, feed conversion ratio, and crude protein digestibility of broiler chickens. Including q...
Shoaib Ahmed Pirzado1,2*, Wu Zhengke1, Adanan Purba1, Chen Jiang1, Huiyi Cai1, Chen Guilan1 and Guohua Liu1*
...ineralization of broiler chickens. A total of 240-day old male chicks were randomly assigned into comprising four treatments with six replicates (n=10), which included control (basal diet), control +0.25% AZO, control + 0.50% AZO and control + kitasamycin (as AGPs). The results indicate that LBGW, ADG and FCR was significantly (P<0.05) improved in AZO 0.25% and 0.50% than the control. Eviscerated (EV), breast muscle (BM) and leg muscle (LM) were significant...

Keiven Mark B. Ampode1*, Federico C. Mendoza2 

...ated immunity of broiler chickens. A total of sixty-day-old Cobb-broiler chickens were used in the study and arranged in a Completely Randomized Design experimental set-up with four dietary treatments. Each treatment was replicated three times, having five birds in every replication. The experimental rations containing graded levels of OP (0%, 1%, 3%, and 5%) were formulated and fed ad libitum in 42 days feeding trial. The r...

Ziyue Qin1, Ali Mujtaba Shah2, 3, Qing Zhu1, Yan Wang1, Diyan Li1, Gang Shu4, Yaofu Tian1 and Xiaoling Zhao1*

...n glucose homeostasis in chickens, we conducted the oral glucose tolerance test, and insulin sensitively test (OGTT and IST) in the fast- (FG) and slow- (SG) growing broilers. Each of 120 one-day-old males was raised in 10 batteries, respectively. In the OGTT test, ten broilers on d 42 fasted 12 h from each stock were randomly assigned into two groups: glucose and vehicle, respectively. The glucose group received a glucose syrup by oral gavage (2 g/kg BW), whi...

Mohamed S. Hussein1*, Hanan A. Fahmy2, Ashraf A. Abd El Tawab3 

...parently healthy broiler chickens in Egypt. A total of 150 cloacal swabs collected from broiler chickens from farms and bird markets were subjected to bacteriological and biochemical examination. The results revealed that 92% (138/150) of samples were found positive for E. coli. The antimicrobial susceptibility testing of 72 confirmed E. coli isolates against seven quinolones and fluoroquinolones antibiotics revealed that 62...

Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2 

... disease particularly in chickens. Thus, this preliminary study was performed to identify intestinal fungal population in immunosuppressed chickens. Cloacal swabs were collected from freshly dead chickens suffering from infectious bursal disease (IBD) for isolation of fungal species on specific medium. A total of 19 purified fungal isolates have been identified morphologically. Aspergillus...

Ho Le Quynh Chau, Than Thi Thanh Tra, Duong Thi Huong, Du Thanh Hang, Le Thi Thu Hang, Vo Thi Minh Tam, Dinh Van Dung*, Ho Trung Thong 

...onik (2010) for coloured chickens. At 12 weeks of age, 8 male and 8 female chicks per treatment were randomly chosen for the carcass yield, meat quality, and myogenic gene expression evaluation (myostatin, myf5, MEF2B). The results showed that the higher level of dietary SID methionine than that of Evonik recommendation (2010) improved growth performance in slow-growing colored chickens but had no effect on body weight gain ...

Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1* 

...| Antimicrobial, Broiler chickens, Conventional, Molecular, Prevalence, Salmonella 

...

Mirza Lena1,2, Dinda Fathia Syahramadani2, Alfi Nurrachma Gustya2, Arif Darmawan2,3, Sumiati2, Wiwin Winarsih4, Minoru Maeda5, Komang Gede Wiryawan2*  

 Mian Abdul Hafeez1*, Adeel Sattar2, Faiza Aslam3, Muhammad Imran3, Kamran Ashraf1, Rashid Zia2 and M. Muntazir Mehdi1

Sajjad Khan*, Naila Chand, Abdul Hafeez, Nazir Ahmad 

...enic bacteria in broiler chickens. A total of 160 day-old Ross chicks were assigned to four groups, containing 0, 0.5,1 and 1.5% GA along with basal feed for 42 days. A completely randomized design (CRD) was followed during statistical analysis while difference in means was calculated through Tukey’s test. Results indicated that supplementation of GA at 1.5% significantly (P<0.05) improved feed intake, body weight gain, feed conversion ratio (FCR), Eu...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...meat products as grilled chickens, grilled beef kofta, and cooked beef meat sold in Assiut city restaurants, and among food handlers working in the same food premises. In addition to the sequencing of 23S rRNA gene and phylogenetic relation between S. aureus isolated from meat products and food handlers. Also, detect the antimicrobial profile and effect of two natural agents of cow milk proteins such as casein and α-lactalbumin on S. aureus isolates of c...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...aturally infected native chickens. Thus, bursa specimens were collected from two IBD vaccinated native broiler chicken flocks showing clinical signs, mortality and lesions of IBD in this study. The collected bursae showed pathological changes related to those of IBD infection. Furthermore, the isolates were molecularly characterized using reverse transcription polymerase chain reaction (RT-PCR) technique and gene sequencing of partial portion of VP2 gene. The ...

Shewangizaw Wolde1,2*, Tadele Mirkena1,3, Aberra Melesse1, Tadelle Dessie4, Solomon Abegaz5 

...owth performance of male chickens and carcass traits of old hens, and genotype and age effects on carcass traits of male chickens. A total of 150 Normal Feathered local chickens (LL), 150 Sasso-RIR (SRSR) and 150 their F1 crossbred (LSR) chickens were hatched and reared on station until 18-weeks of age. Then, 32 males of each genotype were evaluated for ...

Ly Thi Thu Lan1, Nguyen Thi Anh Thu1, Lam Thai Hung2, Nguyen Thi Hong Nhan3, Le Thanh Phuong4, Nguyen Trong Ngu3* 

...s characteristics of Noi chickens. A total of 183 birds were examined in the study. Body weight was recorded individually at days 1, 35, 63, and 91, and carcass parameters were determined at 91 days for all chickens. For determining single nucleotide polymorphisms, the PCR-RFLP method was used with the help of AcyI restriction enzyme. A polymorphic site was found in the population, with the frequencies of 11, 12, and 22 geno...

Husmaini1*, Riza Andesca Putra1, Indri Juliyarsi1, Tevina Edwin1, Linda Suhartati1, Aditya Alqamal Alianta1, Harmaini2 

...actual population of 610 chickens, an effective population of 600 chickens, and an inbreeding rate of 0.08%. The result showed the farmer’s profile 89.47% of respondents was in 25-55 years old, 47.37% respondent was eduucated in elementary school, 42.11% respondents has 5-10 years farming experience, 56.14% respondents distributed in Jorong Koto Tuo, 98.2% respondend raising KCB as a side business, 56.1% of KCB was for...

Taiwo Oladoye Akande*, Dayo Johnson Ogunyemi, Priscilla Funmilola Okunlola, Emmanuel Owolabi, Odetayo Olakanmi 

...the organ weights of the chickens. The fat deposition was substantially reduced (P<0.05) in birds with PFAs. The blood triglycerides, cholesterol, and low-density lipoprotein (LDL) were lower (P<0.05) in groups fed with PFAs. All the PFAs significantly increased the HDL above the value obtained in control. No mortality was recorded throughout the experimentation period. It was concluded that the three PFAs and their mixture used in this study improved th...

Sutopo Sutopo, Dela Ayu Lestari, Edy Kurnianto, Asep Setiaji* 

...of the traits. Male Kedu chickens showed more superior than the female ones. EWG showed positive phenotypic correlations to most of BW measured.

Keywords | Average daily gain, Kedu chickens, Mixed model, Phenotypic correlations 

...

Jumshaid Iqbal1, Muhammad Sharif1*, Muhammad Nadeem Suleman2, Muhammad Saeed3, Fawwad Ahamd1, Asghar Ali kamboh4, Tugay Ayaşan5 and Muhammad Arslan3

...stomorphology in broiler chickens. One hundred and twenty day-old broiler chicks were randomly distributed into four treatment groups with three replicates per treatment (10 chicks per replicate) under Completely Randomized Design. Four iso-nitrogenous and iso-caloric diets i.e. A, B, C and D were supplemented with a natural growth promoter (NGP) at the rate of 0 (control), 1.5, 3 and 4.5g/kg of feed, respectively. Feed consumption (FC) and weight gain (WG) we...

Muhammad Ammar Dilawar1,2, Hong Seok Mun1, Myeong Gil Jeong1, Eun Ju Yang3, Hyeoung Seog Park4 and Chul Ju Yang1,2,*

Aqsa Fayyaz1, Muhammad Kashif Zahoor1*, Asma Ashraf2, Muhammad Asif Zahoor3, Azhar Rasul2, Humara Naz Majeed4, Muhammad Zulhussnain1, Kanwal Ranian1, Bushra Riaz1, Nazia Khalil1 and Attaullah1
...us of domesticated (i.e. chickens, peacock, pet birds) and wild birds, which is excreted in the fecal material of infected birds. The house fly, Musca domestica L. can gain entry into the poultry flocks and will feed on poultry droppings and potentially serves as a mechanical vector for Newcastle disease. During the current study, houseflies were captured and examined for the presence of NDV. A total of 80 samples were collected from eight different poultry fa...
Thobela Louis Tyasi1*, Lebo Trudy Rashijane1, Kwena Mokoena1, Kagisho Madikadike Molabe1, Madumetja Cyril Mathapo1, Victoria Rankotsan Hlokoe1, Lebelo Joyceline Selala1, Masixole Maswana1 and Lubabalo Bila2
...s a reproduction tool in chickens and a valuable food source for humans. The objective of this study was to examine the effect of egg weight (EW) on egg quality traits such as egg length (EL), egg diameter (ED), yolk weight (YW), albumen weight (AW), shell weight (SW), shell index (SI), yolk ratio (YR), albumen ratio (AR) and shell ratio (SR). Potchefstroom Koekoek layer genotype eggs (n = 200) were used. Pearson correlation and analysis of variance (ANOVA) we...

Md. Taimur Islam1*, Mirza Mienur Meher2, Anas Bin Harun3 and Md. Golam Haider1

 

...r and in 0-3 months aged chickens. AMPV generally infects the upper respiratory tract of poultry, which has been isolated for the first time in Bangladesh in 2016. Alternatively, adult chickens are more susceptible to IB and ILT. The prevalence of mycoplasmosis and aspergillosis are higher during the winter season and are reported to occur due to poor management systems. Overall, respiratory diseases are most common in the c...

Anis Suraya Mohamad Abir1, Sakinah Yusof1, Abd Rahaman Yasmin1,2, Abdul Rahman Omar1,3, Kok Lian Ho4, Wen Siang Tan1,5, Abdul Razak Mariatulqabtiah1,6* 

... after FWPV infection in chickens.

Keywords | Fowlpox, Avian influenza virus, Chicken, Dendritic cells, Haemagglutinin 

...

Suhu Duan, Zaiqiang Li, Zhenzhen Fan, Mengran Qin, Xiaoxue Yu* and Liuan Li*

... each group contained 40 chickens that were fed outdoors in an activity area of 63 m2 with free food and portable water. The CK was fed basic diet, and the experimental group added 1%, 3% and 5% linseed oil to the basic diet (TP1, TP2, TP3), respectively. On the 10th, 25th and 40th day of the formal trial, 36 eggs were randomly selected from each group, of which 12 were used to detect egg quality and 24 to detect monounsaturated fatty acids in eggs. The result...

Lopamudra Samantaray*, Yashaswi Nayak 

...e performance of Broiler chickens. The study used 280; 70-day-old chicks. The birds were divided into seven experimental groups; one control and six were fed on phytobiotics EOs at rates of 1% and 0.5% and monitored for 8 weeks. The results revealed that the inclusion of 0.5% black pepper and 0.5% fennel combination, as well as a 1% turmeric, significantly (P < 0.05) improved body weights in birds. Black pepper and a combination of turmeric and fennel revea...

Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1* 

...ral infection in broiler chickens. More field and experimental investigations are crucial to highlight the pathogenesis and the interaction between different respiratory pathogens in chickens.

Keywords | Avian influenza, Broiler chickens, Co-infection, H9N2, Infectious bronchitis Mycoplasma gallisepticum, Mycoplasma synovaie. 

...

Mohamed El-Henfnawy*, Essam A. El-Gendy, Ahamed M. El-Kaiaty, Mostafa Helal 

Tri Untari1*, Sitarina Widyarini2, Michael Haryadi Wibowo1, Marla Anggita1 

...er administration to the chickens. Red ginger powder could provide the highest immunostimulant effect on NDV vaccinated chicken with no toxic effect to the chickens.

Keywords | Antibody response, Immunostimulant, Newcastle disease, Red ginger, Broiler 

...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
...ance from NDV strains in chickens and turkeys which is alarming for the potential transmission of NDVs from quails to other poultry species.
 
Keywords | Newcastle disease, Quails, RT-PCR, F gene, Pathological changes, Class II genotype VII.1.1
...
Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*
... serotype O6 infected in chickens. A total of 420 broilers were randomly assigned to seven treatment groups. The negative control (NC) birds received no E. coli or phages, whereas the positive control (PC) birds were infected with E. coli only. The NC+MHH6 and NC+PR2 treatments received 109 pfu/ml of phages MHH6 or PR2, respectively; whereas the PC+MHH6, PC+PR2, and PC+MHH6PR2 groups were infected with 107.1 cfu/ml of E. coli strain O6 and treated with 109 pfu...

Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2** 

...identified among broiler chickens in Luxor governorate in 2016. Five hundred (n= 500) samples were collected from fifty farms (5 tracheal and 5 cloacal pools from each farm) have been examined by real time RT-PCR for the prevalence of AIV H9. The results revealed that ten farms (20%) were positive for AIV (H9N2). Three isolates with high CT values have been selected for complete sequencing of hemagglutinin gene. Amino acid sequences showed that these isolates...

Nishant Shah1*, Rakesh Kumar Yadav1, Anil Gautam1*, Shambhu Shah1 and Mohan Kumar Gupta2

...r the rearing of broiler chickens for better production.

...
Bulkaini*, Syamsuhaidi, Yusuf Sutaryono, Djoko Kisworo, Sukirno, Sukarne, Tapaul Rozi
...at production of broiler chickens are indicators to assess carcass quality. This study was conducted to evaluate the carcass characteristics and production of pure meat broiler chicken circulating in the traditional markets of Lombok and Sumbawa based on the Indonesian National Standard. The material used was 191 heads of broiler chickens with a slaughter age of 45-49 days. Ninety heads from a chicken slaughterhouse in Matar...
Muhammad Kashif Iqbal1*, Khalid Mehmood2,4, Shakeel Ahmed3, Fazul Nabi4, Muhammad Arif Rizwan1, Muhammad Kaleem1, Mushtaq Ahmed1, Abdul Waheed1 and Jiakui Li5
... growth plate in broiler chickens. So, it is effectual through rectifying the oxidative imbalance and liver damage.

...

Ahmed A. Mohammed1, Madeha H. A. Darwish1, Eman A.A. Negm2, Ayman S. Abdel- Maguid3 

Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5

...rty-five day-old broiler chickens were randomly divided into five treatments and housed under identical husbandry conditions. Antibiotic-free poultry feed was procured from a commercial feed mill. The antibiotics were mixed in the feed as per the manufacturer’s instructions and offered ad libitum to the chickens for the entire study period. The chickens were vaccinated for NDV and AI...
Zahra Naz1,2, Fouzia Ismat1, Muhammad Saleem2, Mazhar Iqbal1, Aamir Shehzad1 and Moazur Rahman1,2*
...ewcastle disease (ND) in chickens. Owing to its highly conserved nature among NDV strains and also due to its pivotal role in the viral life cycle, the M protein can be employed as a promising diagnostic antigen for reliable detection of NDV infection in chickens. In the present study, we have devised a strategy for extraction and solubilization of the NDV M protein from Escherichia coli in a single step using a non-ionic de...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
...ation. Total 120 broiler chickens were randomly distributed in ten groups. The groups included negative and positive control, pure organic acid group and commercial organic acid formulation group. Excluding negative control group, all other groups were orally challenged with 0.1 ml of the 6-Log 10 CFU/ml of the Campylobacter jejuni culture in normal saline via oral route. Cloacal samples were collected for Campylobacter count, body weight (BW) and feed convers...

Muhammad Halim Natsir*, Osfar Sjofjan, Yuli Frita Nuningtyas, Danung Nur Adli, Lizar Basnelda, Dimas Frisky Apriyanto, Wahyu Tri Utami 

...ial population of Lohman chickens. A total 256 Lohmann chicken were used on the experimental diets. The Fountain clerodendrum and avocado seed flour were enhanced with nanotechnology 500 mm. The two-way nested Anova with eight treatments and four replication was employed, with each experimental unit consisting of eight Lohmann broiler chickens. The treatments consisted of B1T0 = basal diet, B1T1 = basal diet + 0.4% avocado s...

Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1* 

...controlling infection in chickens.

Keywords | Curcumin -olive oil nanocomposite, anticoccidial activity, Comet assay, Poultry, coccidiosis. 

...

Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed Elbayoumi1 , Sameh Abdel- Moez Ahmed Amer1, Asmaa Mahmoud Matoaq1 , Mohamed Abdel Aziz Kutkat1, Gomaa Abd El-Rhim Abdel-Alim2  

...o 260 commercial broiler chickens in 13 groups; 20 birds each. At 28 days-old, all groups were challenged with NDV genotype VII 1.1 strain “NDV-CHICKEN-EGY-ALEX-NRC-2020” to evaluate the protective immunity of applied protocols. Humoral immune response, clinical signs, post-mortem gross lesions, growth performance as well as mortalities are all recorded and evaluated. All vaccination protocols were able to induce antibody levels for NDV after vacci...

Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3 

...xed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was i...

Sekobane Daniel Kolobe, Tlou Grace Manyelo*, Jones Wilfred Ng’ambi, Munyadziwa Felicia Dorcus Nemauluma, Emmanuel Malematja  

...t morphology of Ross 308 chickens from day-old to 6 weeks of age. 320-day-old Ross 308 broiler chicks were assigned to a 2 (sex) × 4 (dietary treatment levels) factorial arrangement in a CRD, having 8 treatments, replicated x4 with 10 chicks/replicate. AKLM inclusion levels were at 0, 0.5, 1.0 or 1.5g/kg dry matter (DM). Weekly feed intake (FI), live weight (LW), and growth rate (GR) were measured to obtain the feed conversion ratio (FCR). Gut character...

Ahmed H. Abed1, Ahmed Orabi2*, Wafaa Hassan3, , Enas Hammad4 , Ghada Gaber5 

...iating effect on broiler chickens’ immunity challenged with E. coli. One hundred and twenty broiler chicks were separated into eight equal groups in an in vitro experiment (15 birds for each group). A negative control group was the first group. There were 3 groupings were given immune modulatory agents (lectins, probiotic, and essential oil) for five days without being challenged; Immune modulatory agents were given to the remaining three groups, who wer...

Tlou Grace Manyelo1,2, Nthabiseng Amenda Sebola1, Jones Wilfred Ng’ambi2, Monnye Mabelebele1* 

... of indigenous Boschveld chickens. A total of 200 one-day-old indigenous Boschveld chicks were randomly allocated to five treatments in a completely randomized design each with four replicates of ten chicks. Amaranth leaf meal (ALM) levels evaluated in this study were 0, 5, 10, 15, and 20% with recording weekly body weights, feed intakes, and feed conversion ratios. Gut organ weights, lengths, pH, and meat characteristics were determined, and the general linea...

Hemat A. Abdel Magied*, Heba H. Habib, Amany H. Waly, Ahmed Fadl, Sayed M. Shalash 

...y of Arbor Acres broiler chickens. Two experiments were conducted in current study; experiments 1 (EXP1) evaluated the effects of PE and/or P supplementation to standard corn-soybean broiler diet (STD). while experiment 2 (EXP2) examined the PEP supplemented to different levels of low CP (low 10 or 15 %) and low ME diets (lower 100 or 150 kcal/kg) than control (L10%-100, L10%-150, L15%-100, L15%-150). The obtained results were: the EXP1 showed that significant...

Andy1,4*, Ratmawati Malaka2, Sri Purwanti3, Hikmah M Ali2, Tutik Lusyta Aulyani4, Danung Nur Adli5 

...obial profile of broiler chickens. A total 100 broiler chickens were reared for five weeks on the experimental diets. A general linear models with five treatments and four replications was employed. The treatments consisted of T0 = control, T1 = basal diet + acidal, T2 = basal diet + 0.5% encapsulation of liquid smoke, T3 = basal diet + 1.0% encapsulation of liquid smoke, T4 = basal diet + 1.5% encapsulation of liquid smoke....

Sugiharto Sugiharto*, Ikania Agusetyaningsih, Endang Widiastuti, Hanny Indrat Wahyuni, Turrini Yudiarti, Tri Agus Sartono 

...n CONT, HSD-P and HSD-PC chickens consumed more (P < 0.05) feed than HSD chickens. Body weight and weight gain were lower (P < 0.05) in HSD compared to the CONT, HSD-P and HSD-PC groups. The European Production Higher Efficiency Factor (EPEF) tended (P = 0.06) to be higher in HSD-PC than in HSD chicks. While there was no significant effect of the treatments on complete blood counts and serum biochemical parameters, ser...

Istna Mangisah1*, Heni Rizqiati2, Nyoman Suthama1 

...starter. Day-old broiler chickens with an average body weight of 45.61 ± 3.46 g, a total of 160 birds, were placed evenly in 20 experimental plots. The treatment tested was the LDTE level in 100 grams of feed, LDTE0 = without LDTE, LDTE1 = 1 mL, LDTE2 = 2 mL, and LDTE3 = 3 mL. The treatment was given for 21 days, starting from d-1 until d-21. Intestinal microbial population, weights of digestive and immune organs, nutrient digestibility, and growth per...

Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*

... (n=504) day-old broiler chickens procured randomly allocated into 6 treatments dietary treatments were: B positive control (PC) diet (0.42% available phosphorus in grower feed, A negative control (NC) diet with (0.30% avail. P in starter and 0.26% avail. phosphorus in grower) while four diets C, D supplemented with NC+ (500 and 750 powder phytase units (FTU)/kg) and G, H supplemented with NC+ (500 and 750 liquid phytase units (FTU)/kg) respectively. Pelleting...

Ahmed Rizk1, Gomaa Said Ramadan2*, Alaa Eldin Abdou3, Ossama Ahmed El-Weshahy1, Omer Sayed Rashed1 

... Tanta G- 1 and Mamourah chickens strain respectively. In the first generation age at sexual maturity for Tanta G- 1 was 172 days, while it was 157 days for the Mamourah strain. The weight of the first egg weight was (45.46g) for Tanta G- 1, while it was 34.84g for the Mamourah strain (34.84g). The overall mean fertility percentage for Tanta G-1 female egg was 87.92 %, while it was 88.94 % for the Mamourah strain and the difference was significantly differen...

Reda Hassan*, Bahaa Abou-shehema, Sherif Zayed, Micheal Gorgy, Shama Morsy, El-Sayed Abu El-Hassan, Mahmoud El-Gbaly, Hanaa Basuony, Ebtehal Hassan 

...cks). On the other hand, chickens were offered the basal dietas Negative Control (NC, group 1); the other three groups 2, 3, and 4 were fed NC supplemented with 0.5% HSCAS, SC and HSCAS plus SC respectively, group 5 chicks were fed a basal diet contaminated with 1ppm AFB1 as Positive Control (PC), the other three groups 6, 7 and 8 were fed PC supplemented with 0.5% HSCAS, SC, and HSCAS plus SC respectively. Results exhibited that broiler chicks fed with a PC d...

Pham Tan Nha*, Le Thu Thuy  

...the performance of local chickens in the south of Vietnam. It was (3*2*3 factorial) with 2 factors. Factor fermentation: Yeast-fermented maize (YM), Yeast-fermented broken rice (YR), Probiotic fermented maize (PM). Factor protein level: 20% (CP20) and 16% (CP16). One hundred and eighty Noi chickens at 6 weeks of age (334±9.5 g/bird), and 10 birds per unit (balanced sex) were in the treatment. All treatments were added...

Andi Tenri Bau Astuti Mahmud1, Lellah Rahim2, Muhammad Ihsan Andi Dagong2*, Sri Rachma Aprilita Bugiwati2, Wempie Pakiding2 

...ual feed intake (RFI) in chickens, which is usually calculated during the growth period, is a measure of feed efficiency that does not depend on the level of production. The study aimed to evaluate the growth performance, carcass quality, physical meat characteristics, and sarcomere properties of Kalosi chicken, which were grouped according to high and low Residual Feed Intake (RFI) phenotype. This study used 64Kalosi chickens

M. Mansoor Javed*, M. Aslam Mirza, M. Aziz ur Rehman and M. Saif ur Rahman

...on and growth in broiler chickens. Full-fat soybean was prepared by subjecting dehulled soybean to different temperatures i.e. 90°C, 100°C and 110°C for various resident tines i.e., 15 min, 30 min and 45 min in a multitier commercial toaster. Each product was analyzed for proximate composition, pepsin digestibility, KOH solubility and urease activity prior to inclusion in the experimental diets. A day old Cobb-500 broiler birds (n=432) were purchas...

Sri Setyaningrum*, Dini Julia Sari Siregar, Tengku Gilang Pradana  

...ut microflora of broiler chickens. The research used 144 one-day-old broiler chicks (44.33±1.09 g). The research was conducted with a completely randomized design. The treatments included of T0: diet + 0% CGLp (control), T1: diet + CGLp 1.0%, T2: diet + CGLp 1.5%, T3: diet + CGLp 2.0%, T4: diet + CGLp 2.5% and T5: diet + CGLp 3.0%. The treatment of CGLp was significantly increased (p <0.05) of body weight gain, live body weight, carcass percentage, m...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...nged to the W-36 Hy-Line chickens. The layers were randomly designated into five equal treatment groups (9 replicates × 6 hens per replicate for each treatment) according to the dietary inclusion levels of BSFL meal. The first group of birds were fed a basal diet of soybean-corn meals and served as a control (0% BSFL meal), while the remaining 4 groups were fed a basal diet in which the soybean-corn meals were partially replaced with 3%, 6%, 9%, and 12% ...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Mohammad A. Al-Mahaish4, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel5
...ic efficiency of broiler chickens. The study included 360 unsexed Cobb500™ commercial broiler chicks aged 1 day and weighing 46±1.5 g. Starting from the 22nd to the 42nd d of age, the chicks were randomly distributed according to the dietary inclusion levels of MWM into six treatment groups (6 replicate pens per group × 10 chicks per replicate, considering the equal ratio of males to females in each replicate). The first group of birds was f...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed El-Bayoumi1, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila1, Gomaa Abd El-Rhim Abdel Alim2
...;) in commercial broiler chickens. The pathogenicity studies parameters included the effects on protection, growth performance and clinico-pathological changes. The results indicated that, immunostimulants can keep maternal immunity longer where lector® was the best; the decline in HI titers also indicates that bird groups not contract ND natural infection. The results of performance parameters after immunostimulants administration and challenged with IBDV...
Fatma Salih Mohammed1*, Nasser Khedr1, Tahia Ahmed1, Liza S. Mohammed2
...ne-day-old Ross® 308 chickens were randomly distributed into three groups with different dietary treatments (66 birds per group). The dietary treatments were as follows: D1, control (soybean meal 46%); D2, D1 + EE-SBM (50 kg starter, 100 kg grower, and 200 kg finisher/ton); and D3, D1 + EE-SBM (100 kg starter, 150 kg grower, and 250 kg finisher/ton). The study results showed that the addition of EE-SBM to broiler diets had no significant effect on carcass ...

Yusri Sapsuha1*, Nur Sjafani1, Suryati Tjokrodiningrat2

...e performance of broiler chickens is focused on maximizing performance parameters, i.e., fast chicken growth with low feed consumption, and with optimal health status. This study provides an in-depth review of the use of cloves in feed to improve broiler chicken production performance and health. In conclusion, cloves contain bioactive substances that can be used to improve broiler chicken performance and health. Administration of appropriate dose is believed ...

Abdul Azis*, Afriani Afriani

...lications. As a control, chickens were fed ad libitum (FR-0); chickens were given free access to feed during two periods of 2 hours from age 7-14 days (FR-1) and age 7-21 days (FR-2); chickens were given free ac­cess to feed during two periods of 4 hours from age 7-14 days (FR-3) and age 7-21 days (FR-4). Body weight, carcass yield, breast, and tight muscle weight of broilers subjected...

Ahmed Ali Chandio1, Asghar Ali Kamboh1*, Nazar Ali Korejo2, Riaz Ahmed Leghari3 and Muhammad Ali Chandio4

Riko Noviadi, Dwi Desmiyeni Putri, Gusma Gama Maradon*, Agung Adi Candra, Nani Irwani, Gadis Apriani, Made Guntur Candra Adinata, I Made Krisnanda

Doungnapa Promket1,2,4*, Khanitta Pengmeesri2,4, Jennarong Kammongkun3, Thassawan Somchan2

...raits in 300 Thai native chickens were investigated. DNA was extracted from blood samples for genotyping using specific primers and restriction enzymes for each gene, and polymerase chain reaction-restriction fragment length polymorphism was used to identify the genotypes (PCR-RFLP). Three genotypes were found for each gene as BB, Bb and bb for NPY; TT, TC and CC for DRD2 and II, ID and DD for VIP. Genotype frequencies of NPY (range 0.13-0.58), DRD2 (range 0.0...

Zulaikha Zainool Abidin1, Fahim Hafiy Idris2, Suriya Kumari Ramiah1, Elmutaz Atta Awad1, Zunita Zakaria3,4, Zulkifli Idrus1,2* 

...bacillus spp. in broiler chickens. Thirty-five-day-old broiler chickens were subjected to either 0 h or 8 h of feed withdrawal, followed by 2 h or 4 h of road transportation. For each feed withdrawal-transportation subgroup, the birds were lairaged for 0 h or 3 h. Salmonella spp. and Campylobacter spp. were only detected in two and three birds, respectively. Because of the insufficient data, both types of bacteria were exclu...

Hendy Musbawandi1, Mohammad H. Tamzil2*, Budi Indarsih2 

...domized design using 180 chickens. All chickens were divided into three groups with six replicates each which were the first group was reared in a closed house, the second and third groups were reared in positive pressure and open houses. Each replicate, in each treatment, was reared in a different barn plot with the number of 10 chickens/m2 of the barn area. The results showed that feed c...

Tanvir Ahmed1, Sachchidananda Das Chowdhury1, Bibek Chandra Roy1Shubash Chandra Das1*, Khan Md. Shaiful Islam2, Bipul Chandra Ray1, Sukumar Saha3 

...mune response in broiler chickens. A total of 450 Cobb-500 unsexed DOCs were divided in response to the treatment into broilers fed a control diet (no additives), control supplemented with PB (100 g/100 kg feed), or control supplemented with AGP (Lincomycin 2.2%, 15 g/100 kg feed), having six replications in each treatment (25 birds per replication). Growth parameters were recorded. On days 22 and 34, HI antibody titer was determined against Newcastle disease ...

Muhammad Arshad Iqbal*, Aamir Ghafoor, Irfan Ahmad, Muhammad Ijaz 

...f 180 commercial broiler chickens were obtained from local hatchery and were reared for 42 days. Three groups (A, B, C) of birds were divided into 3 subgroups with 20 birds in each group. Group A (-ve control) received diet without AGP or probiotic. Group B (+ve control) was fed with AGP. Group C was probiotic feed supplemented with B. amyloliquefaciens. All subgroups 1 were kept non-challenged, at day 21, subgroups 2 were challenged with 1x105 CFU of S. Enter...

Maria Endo Mahata1*, Oriyanti Br Situngkir1, Yan Heryandi2, Takayuki Ohnuma3, Yose Rizal1 

...dy used 30 broiler male chickens with an average body weight of 1.5 kg at five weeks of age. This study used an experimental method with a completely randomized design (CRD) of a 3x3 factorial pattern which was repeated three times. Factor A (type of pellet binder): A1 (brown seaweed), A2 (taro tuber), and A3 (tapioca flour), then factor B (dosage of pellet binder): B1 (1.5; 3.3; and 4.5%). Crude fiber digestibility, nitrogen retention, and energy metabolism ...

Abdulaziz A. Alaqil

...on. Four hundred broiler chickens (Cobb500, 1-d-old) were randomly distributed in equal numbers in 40 battery cages (10 birds per cage). Every ten cages were placed in a room to arrange four experimental room groups identified as C, EC, PR, and PR+EC groups, respectively. From 22-42 d of age, the C and EC groups have received a basal diet with no PR supplements, whereas the PR and PR+EC groups have received PR at 1 g/kg of the basal diet. On day 36 of age, the...

Md Tariqul Islam1,5 *, Aditya Chowdhury Avi1,5, Md Saiful Bari2,5, Most Rebeka Sultana Swapna Khandoker3, Homaira Pervin Heema4, Goutam Buddha Das1 

...aracteristics of broiler chickens. In a 28-day study, Investigation was carried out using 120 Ross-308 unsexed day-old chicks (DOC) of broiler chicken. Birds were selected by random selection to assign them to each of four feed trial groups: T0, T1, T2, and T3, and each of those received 0, 20, 30, and 40% PM supplementation. The result showed that no significant variations were observed in survival rate, feed conversion ratio, or carcass yield within the broi...

Advances in Animal and Veterinary Sciences

June

Adv. Anim. Vet. Sci., Vol. 11, Iss. 6, pp. 864-1036

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