Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
... three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4°C for 2 h and equilibrated for 4 h at 4°C. Straws were then kept over liquid nitrogen vapours for 10 min and plunged in liquid nitrogen fo...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...ed out on Achai breeding bulls of similar age at the Semen Production Unit, Harichand, District Charsadda, Khyber Pakhtunkhwa. Collection of semen was done using artificial vagina (42°C) for three weeks. Semen was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 and 1.0%) of green tea extract. Testing was consummated for quality characteristics of post-thawed spermatozoa such as motility, viability and plasma membrane inte...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
... from two mature buffalo bulls, diluted in tris citrate egg yolk based extender and cryopreserved using standard protocol. The semen straws were thawed at 37oC and stained using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II (heating effect during incubation in tampon solution); smears were immersed in tampon either at room temperature or at 60...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...ere collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane integrit...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...mfortness experienced in bulls during semen collection by electro ejaculation method. The normal electro ejaculation method of semen collection (Method I) was compared to a modified method involving three stages of graduated electrical stimulation (Method II) in four crossbred bulls. The results showed that intensive muscle spasm, bull struggling and arc back were reduced (p ˂ 0.05), as well as the time of penile protru...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...
Nine healthy mature bulls having average age 3 ± 0.14 years, were selected in three different groups i.e., Sahiwal, Holstein-Friesian and crossbred (Sahiwal x Holstein-Friesian) to compare the semen quality. Semen samples were individually diluted with egg yolk extender and tested for individual motility, progressive motility, plasma membrane integrity, acrosomal and DNA integrity, seminal pH and dead sperm percentage in fresh and thawed sem...
Relationship of PIT-1 Gene Polymorphism with Breeding Parameters and Body Weights of Cows and Calves
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...-White Holstein-Friesian bulls and giving birth to single calf. The following information was taken from each animal: age at first insemination, age at first calving, number of semen portions attributable to successful insemination, length of pregnancy and periods of: insemination service, postpartum resting period, interpregnancy period and intercalving period. The study also determined weight of the cow and weight of the calf at calving (including gender). S...
İbrahim Aytekin1,*, Ecevit Eyduran2, Koksal Karadas3, Rifat Akşahan4 and İsmail Keskin1
...im, a total of 103 young bulls were used. In the prediction of fattening final live weight as an output variable, several continuous predictors evaluated in the current study were: withers height (WH), back height (BH), front rump height (FRH), back rump height (BRH), body length (BL), back rump width (BRW), chest depth (CD) and chest circumference (CC). Also, the breed factor was considered as a nominal predictor and fattening period (FP) was accepted as an o...
Ewa Czerniawska-Piątkowska1,*, Magdalena Skibicka1, Barbara Cioch-Szklarz1, Jolanta Karakulska2, Karol Fijałkowski2 and Sonia Hiller3
... and traditional rearing bulls had a larger mass than heifer calves. In traditional rearing, results of analysis of the swabs from the nasal cavities revealed the presence of pathogenic or opportunistic bacteria at the level of the average number of micro-organisms (11-50 CFU) or high (> 50 CFU), which negatively affected the health of the calves. However, in cold rearing there was recorded absence or presence of microorganisms in the nasal cavity in a smal...
M. Tariq Javed1, Nazir Ahmad2, Gulbeen Saleem3, Haleema Sadia4, Farkhanda Manzoor5 and Ghulam Hussain Dilbar
EFFECT OF AGE AND SEASON ON SEMEN CHARACTERISTICS OF BUFFALO BULL - A REVIEW
...aracteristics in buffalo bulls which will be helpful to researchers and allied professionals in future research or policy planning. The review is bifurcated in different sections for easy understanding of the facts. The review suggests and effect of age and season on buffalo bull semen characteristics. ...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
...emen quality of breeding bulls. A total of 8 adult (≥2 year) bulls (2 each from Jersey, Friesian, Sahiwal and cross bred) were selected with body condition score (BSC) at a scale of (0-5). The results showed that mean semen volume was significantly (P<0.05) high in Friesian bull. Sperm concentration and motility were significantly (P<0.05) high in Jersey bull while live sperm percentage was significantly (P<0.05)...
Naimat Ullah1, Sarzamin Khan2, Muhammad Mobashar3, Shahbaz Javaid4*, Tarique Tonio1 and Asif Mahmood5
...iesian Sahiwal crossbred bulls during summer season. A total of 24 Friesian Sahiwal crossbred bulls at nearly similar age and body weight were selected and allotted erratically into 3 treatment groups, denoted as A, B and C for a period of 2 months (May and June). Each group was assigned 8 bulls. Bulls in group A were not fed with any dietary fat (contro...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...stan. Semen of six adult bulls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental material at Government Cattle Breeding and Dairy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 42°C temperature. Gross and microscopic examination was carried out subsequent to the semen collection. Semen specimen with more than 70% motile characte...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buserelin acetate (Buserelin® aniMedica, Poland). After equilib...
Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2
A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
...rs of buffalo and cattle bulls were recorded. Average number of 5.55 goats per households was reported by 35% of all the households. On an overall basis, 2,365 liters of buffalo milk was computed from 600 households, while 1,033 liters (43.7%) was used in households and 1,332 (56.3%) liters was sold. About 1,868 liters of cow milk were computed from 600 households, while 915 liters (49%) of milk used in households and 953 (51.0%) liters were sold. Majority of ...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...ted from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 billion per mL conc., >60% motility) and cryopreserved using standard procedures. Percent sperm motility, liveability...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2, Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5, Hamayun Khan1 and Shabana Naz6*
...ize: small;">Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...acteristics in crossbred bulls.
...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...s were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo bull sperms. The antioxidative effect of GT was observed at the comparatively lower concentration (0.1-1.5%...
Mohammed Sirajul Islam1,2*, Nurhusien Yimer1*, Wahid Haron1 , Faez Firdaus Abdullah Jesse1, Mark Hiew Wen Han1, Mamat-Hamidi Kamalludin3, Wan-Nor Fitri1, Ubedullah Kaka4, Abdul Quddus1,5
...K and KK × Brangus bulls. Ten (10) experimental bulls with similar ages were kept under the same paddock and fed with equal quality and quantity Guinea grasses and palm kernel cakes. Body weight was measured at monthly basis by using a digital animal weighing scale. Age at sexual maturity was determined by the time of semen ejaculation and quality. T...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...ealthy Nili Ravi buffalo bulls was collected through artificial vagina and Gallic acid was added to the semen at 1 µM, 15 µM, 30 µM, 45 µM, 60 µM, and 100 µM. A total of six groups were prepared. One group was kept as control where no Gallic acid was added. After the addition of extender, semen was cooled to 4°C, filled in 0.5mL straws for 4 h and frozen in liquid nitrogen at -196 °C. The parameters evaluated were pe...
Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr
...and high fertile buffalo-bulls. Also, the relationship between protein profile in BP of adult bulls and bull calves were studied. Blood samples were taken from 3 calves (128.33±7.58 kg and ageing 6 mo.) and 10 bulls (400±37.5 kg and 24-25 months of age) and semen samples from bulls. Semen was collected once a week from all
Hamdy Abdala Elnagar, Wael Mohamed Wafa*, Abdelaziz Mustafa Sakr, Mohammed Mahmoud Hegazy
...r weight of buffalo-male bulls.
Keywords | Buffalo-males, Blood, Testosterone, Leptin, Prolactin.
...
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
...emen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63±16.54% and 52.76± 8.88%, and 53.00±7.93% and 54.08...
Nurul Isnaini*, Akhmad Kurniawan, Muhammad Nur Ihsan, Dedes Amertaningtyas, Artharini Irsyammawati, Faizal Andri
...0) and Brahman crossbred bulls (n=10) were raised under oil palm and cattle integrated farming system. Data were collected for cow and calf performance from two consequent calvings that were distributed into two groups according to the rainy days and rainfall for a year backward from the calving date. The rainy days were categorized as low (< 150 d) and high (≥ 150 d) whereas rainfall was categorized as low (< 2400 mm) and hig...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2
...ntial to become superior bulls to contribute to the strategies for developing Bali bull as a national beef bull contributor.
Keywords | Bali-polled bull, Characteristic, Kinematics, Sperms
...
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
...p>Administration GnRH to bulls is expected to improve semen quality. This study aims to determine the effect of giving the hormone GnRH in various doses on the quality of semen in Ongole Crossbreed (OC) bulls. The study used 18 OC bulls with three GnRH treatments, A (one dose/5ml/animal), B (half-dose/2.5ml/animal), and C without GnRH. The GnRH was administered once by intramuscular (IM) i...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
... preservation in buffalo bulls at animal production research institute, Sakha, Kafr El-Sheikh Governorate, Egypt. The experimental protocol was designed as four groups (Control, G1, G2 and G3). Buffalo-bulls’ semen were exposed to different concentrations of garlic extract (0, 3, 6 and 9%, respectively) to evaluate the semen quality parameters after cooling and freezing process. Data obtained showed that, progressive m...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viabi...
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...fferent ages. Three Aceh bulls were involved in this study. The semen was collected from each bull aged 6 to 10 years and the fresh and frozen semen quality was evaluated directly. The results showed that the bull age significantly affects semen volume, pH, sperm concentration, total sperm, total motile sperm, and frozen semen production of Aceh cattle (p < 0.05). However, it was found that there was no significant change in the sperm motility, before freez...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
...ected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was filled in 0.5 ml French straw at 4°C and kept over liquid nitrogen vapors for 10 min. Then straws were plunged into liquid nitrogen (-196°C) ...
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
...South Sulawesi. Two Bali bulls and two Simmental bulls aged 5-6 were used. The parameters observed in the fresh semen were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were motility, viability, abnormality, intact plasma membrane, acrosome status, DNA integrity, and movement patterns. Viability and abnormality parameters were s...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
...the development of local bulls and Indonesia is no exception, especially in South Sulawesi Province. One of local bulls in which developed for approximately last ten years is Bali-polled bull. Thus, the selection of polled bulls is crucial, mainly in modern livestock management. The aims of this study to identify sperms post-thawing quality and proteomic plasma semen as fertility performan...
Gulzhan Kalenovna Mussayeva1*, Alikhan Meldebekovich Meldebekov2, Nurgul Alikhanovna Meldebekova2, Gulshat Imanzhankyzy Shaykamal1, Batyrkhan Azimkhanovich Buralkhiyev2, Nurkuisa Mirzabekovich Rametov3 and Kanat Zhumanov4
... artificial insemination bulls from the line whose offspring are most productive and physiologically more adapted to the climatic, technological and forage conditions of the farm. When selecting, special attention should be paid to the strength of the hoofed horn and the duration of commercial use. For the given purposes it is recommended to use such bulls of the Wis Burke Ideal line whose average milk productivity exceeds t...
Gilyan V. Fedotova1,2, Marina I. Slozhenkina1, Ivan F. Gorlov1, Aizhan A. Kaidulina1, Alexander A. Mosolov1, Daria A. Mosolova1, Mohammadreza Poorghasemi3 and Alireza Seidavi3*
...Kazakh white-headed (IV) bulls were housed and fed under the same conditions. The bulls used were all classed as Elite. The feeding rate was intense. Steers in all groups were raised in the feedlot throughout the year. Growth rate of the crossbred steers was greater than the purebred steers. This was reflected in higher yields of the most valuable parts of the carcasses, the rump and saddle. Simmental crosses were distinguis...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
...-Belgian-Blue-Crossbreed-bulls aged four years old were kept in a mooring cage and served with forage and concentrate (CP 16%) at 10% and 3% to the body weight, respectively. There were also added some egg yolk citrate diluent of 2.9% (v/v), Sodium citrate dihydrate buffer, 100 mL of distilled water, and an egg yolk mixture to a proportion of 20% egg yolk 8% (v/v) glycerol (Merck, Germany), penicillin (1,000 IU/mL), and streptomycin (1,000 mg/mL). Sperm was di...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
... weeks from five buffalo bulls aged 3.5–5 years. The collected semen was pooled and distributed into five equal fractions, each fraction was diluted with Tris-extender at each level of PEE (PEE0, PEE1, PEE2, PEE3, and PEE4). Semen was gradually diluted at 37 °C with each extender at a rate of 1: 10, equilibrated for 4 hours at 4 °C, packaged in French straws, and frozen in liquid nitrogen at -196 °C. Evaluation of semen was achieved p...
Ebban Bagus Kuntadi1, and Amam Amam2*
...ing heifers, steers, and bulls. The observation involved 4,238 Brahman Cross cattle. The data were analyzed using descriptive analysis and independent sample t-test using SPSS 26.0 software. The results revealed that the weight loss of the imported cattle on the MV Diamantina ship was 12.92 ± 5.66 kg per cattle, Barkly Pearl was 9.98 ± 5.05 kg per cattle, and MV Gudali Ex was 14.32 ± 4 .20 kg per cattle, while the weight loss of Brahman Cr...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...cifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice weekly at the Semangga District Animal Health Center from August to September 2023. Semen diluted to a minimum of 25 million/mL is packaged in 0.25 mL straws. Treat Andromed® as a comparison, P0 tris egg yolk (TYE), P1 TYE+MYE 1 mL, P2 TYE+MYE 2 mL. The parameters observed include fresh semen quality, sperm motility, sperm viability, sperm membrane integrity...
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