Muhammad Zahid1, Muhammad Hamid Bashir1*, Bilal Saeed Khan1 and Muhammad Shahid2
...sucking pests. Different biocontrol agents are being used for the management of these sucking insect pests. Neoseiulus barkeri (Acari: Phytoseiidae) has been proved as an efficient acarine predator of sucking insect pests of different crops. This predator has also been reported from different localities of Pakistan. The main objective of this study was to screen out locally used pesticides i.e., pyriproxyfen, acetamiprid, chlorfenapyr, diafenthiuron and thiacl...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...to be the most efficient biocontrol agent by parasitizing the maximum number of eggs, whereas D. oviparasitica appeared to be the least efficient after 72 hours. The greatest numbers of cysts and eggs were found to be colonized with L. muscarium during microscopic observations. In the greenhouse experiment, L. muscarium had significant effects in reducing the nematode population in soil compared to the other treatments. In regard to the growth parameters,...
Sumra Ashraf1, Zain ul Abdin1,*, Saqi Kosar Abbas2,Rao Sohail Ahmad Khan3, Muhammad Tahir1, Sehrish Rasool1, Maryam Anwar1 and Fiaz Hussain1
...earing of this potential biocontrol agent which may be used in the successful implementation of biocontrol program for the suppression of pyralid pest insects damaging different agricultural crops of economic importance.
... 
Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram
Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
... germplasm collection of biocontrol agents were tested for their antagonistic effect on Colletotrichum gloeosporioides and C. capsici by dual culture test. Among the isolates of chilli microflora tested, Aspergillus flavus showed 70.2% inhibition of C. gloeosporioides and 54.9% inhibition of C. capsici.Among the isolates of Trichoderma sp. tested, T.virens (Tvs-KSD isolate) and T. pseudokoningii (Tpk-1) showed highest percent inhibition of C. gloeosporioides (...
B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran
Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...ned maximum attention as biocontrol agent due to the fact that it is effective against a large number of soil-borne plant pathogenic fungi, suppressive effects on some root nematodes without adversely affecting beneficial microbes like Rhizobium and capable of promoting growth of certain crops. There are two major methods of inoculum production of Trichoderma spp. viz., solid state fermentation and liquid state fermentation. In solid fermentation, the fungus i...
Sitansu Pan and Someshwar Bhagat
Biological control of plant diseases
...A considerable number of biocontrol agents have been established successfully for their bioconrol potential including strains belonging to bacterial genera such as Agrobacterium, Pseudomonas, Streptomyces and Bacillus, and fungal genera such as Gliocladium, Trichoderma, Ampelomyces, Candida and Coniothyrium. Trichoderma and Pseudomonas fluorescens are the most important and widely used bioconrol agents being used across the world for the management of several ...
Debjani Chowdhury, P. C. Paul and B. Dasgupta
Management of leaf spot of Centella asiatica (Thankuni) caused by Alternaria sp. and target leaf spot of Rauvolfia serpentina (Sarpagandha) caused by Corynespora cassicola
...litox at 0.25%) and four biocontrol agents (Trichoderma viride at 107 cfu / ml, T. harzianum at 107 cfu / ml, Pseudomonas fluorescens at 108 cell / ml and Biomonas-a formulated product of P. fluorescens preparation at 0.15% for three times at an interval of 15 days. The results revealed that the lowest percent disease incidence (PDI) of leaf spot of Thankuni caused by Alternaria sp. and highest yield in number of leaves per sq.M was recorded where carbendazim ...
A. Regupathy and R. Ayyasamy
Initiatives of papain industry by private-public-farmer link-ages in classical biocontrol program for papaya mealybug in Tamil Nadu
...ed for getting effective biocontrol agent [Acerophagus papayae (Noyes and Schauff)] through ICAR-NBAII (National Bureau of Agriculturally Important Insects)-TNAU was emphasized as the existing biocontrol agents could not give relief. Later, when the exotic parasitoid was imported by NBAII, Bangalore, the papain industry wholeheartedly supported the training programmes by deploying its field staff in release and multiplicatio...
Goutam Mondal
Plant growth promoting activity of some indigenous Tricho-derma isolates and their field performance against sheath blight of rice in old alluvial zone of North Bengal
...f North Bengal and their biocontrol activity were tested in dual culture and found effective against soil-borne plant pathogens, namely, Fusarium oxysporum f. sp. ciceri (Padw.) Matuo. and Rhizoctonia solani Kühn. Efficacy in plant growth promoting activity of all the isolates was also tested on cauliflower (Brassica oleracea var botrytis L.), chilli (Capsicum frutescens L.) and tomato (Lycopersicon esculentum Mill.) with culture filtrate of
Someshwar Bhagat and Sitansu Pan
Comparative ecological behaviour of some pre- and post-tsunami isolates of Trichoderma harzianum and T. viride from Andaman & Nicobar Islands
...gical behavoiur of these biocontrol agents. All the isolates showed better parasitic ability and rhizosphere colonization when the competition of other organisms withdrawn compared to natural and sun dried soil. Both competitive parasitic behaviour and rhizosphere colonization was low in post-Tsunami isolates of Trichoderma. The chlamydospore inoculum was found best in percentage colonization of sclerotia of boh R. solani and S. rolfsii, followed by mycelial a...
Dhananjoy Mandal 2K. Baral 3M. K. Dasgupta
Developing site-specific appropriate precision agriculture
..., pest management, (BCA) biocontrol agent balance, and energy balance, economics ~ each with several variables. We are also providing ground and prepare to provide PA –specific data to the farmers. By Return per Rupee invested Rice-Potato-Pumpkin1, Cucumber-Cabbage-Basella alba, and Groundnut–Brinjal+Brinjal sequences were suitable for resource-rich growers, whereas Okra-Chilli+Chilli2 , and Black gram–Parwal+Parwal sequences were suitable fo...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...s for food preservation/ biocontrol, this study was designed to isolate lactoferrin from milk of camel. Cation exchange resin (CM-Sephadex C-25) was used for the isolation of lactoferrin using the fast protein liquid chromatography. The lactoferrin was further characterized through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Disc diffusion method was applied for the evaluation of antibacterial activity of lactoferrin. Minimum inhibit...
Gadeeyya G and Ratna Kumar P.K.
...ich may became promising biocontrol agents. A total of 7 weed plants include Commelina benghalensis, Cyperus rotundus, Crotalaria verrucosa, Digera muricata, Sida cordifolia, Ipomoea pestigridis and Trianthema portulacastrum were selected for in vitro studies. Fungal isolates namely Ascochyta cypericola, Bipolaris sp., Chaetomium globosum, Colletotrichum capsici, Colletotrichum sp., Curvularia lunata, Curvularia tuberculat...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...identify alternates like biocontrol agents, plant extracts etc. In the present study the extract of Citrullus colocynthis in different solvents were evaluated on second instar larvae of H. armigera under laboratory studies for toxicity, sublethal and antifeedant effect. The result showed that ethanol based extract was the most effective to control the H. armigera followed by the ethyl acetate. The sublethal concentration of ethanol ...
Hina Safdar1,*, Nazir Javed1, Sajid Aleem Khan1 and Muhammad Arshad2
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1, Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
...>Baculoviruses are known biocontrol agents of several crop pests, however, their activity is deteriorated rapidly when exposed to the sunlight. Many UV protectants were used to improve the efficacy of these viruses under field conditions. The objective of the current study was to evaluate the effectiveness of plant extracts to improve the persistence of Spodoptera littoralis multiple nucleopolyhedrovirus (SpliMNPV) against beet army worm, Spod...
Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1
Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1
...acteria can be used as a biocontrol agent to manage other fungal diseases of agricultural crops also. ...
Imran1*, Amanullah1, Muhammad Arif1, Zahir Shah2 and Abdul Bari3
Sumaira Akram1, Sajid Aleem Khan1*, Nazir Javed1 and Saeed Ahmad2
...t egg hatching and among biocontrol agents Paecilomyces lilacinus (Thom) Samson (PL) produced highest egg hatching while, Trichoderma harzianum Rifai (TH) caused lowest egg hatching. In plant extracts the highest egg hatching was recorded in clove at S concentration and lowest by neem extracts at S/10 concentration. Cartap and rugby produced 100 % juvenile mortality at S concentration. After 48 h the treatment TH + PL caused highest juvenile mort...
Sohail Ahmed1,*, Muhammad Waseem Saleem1, Abid Ali1, Muhammad Shahid Nisar2, Rashad Rasool Khan1 and Abdul Rashid3
...d out the integration of biocontrol agents with plant chemical and insecticide against Thrips tabaci L. (Thripidae: Thysanoptera), release of Coccinella septempunctata and Chrysoperla carnea, spray of Neem Seed Kernel Extract (NSKE) 5% and spinosad 240 SL (Tracer) were integrated in all possible combinations, thus, making a total of 15 treatments including separate application of each above and a control where none was applied except water...
K. Abbasi 1 , R. Wick2† and D. Zafari1
Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis
...f the four isolates. The biocontrol potential of 10 selected isolates was assayed in water-agar in vitro and under greenhouse conditions. L. muscarium had the most potential and Fusarium solani was the least based on the number of parasitized juveniles and eggs in water-agar. Greenhouse trials showed T. atroviridae and B. bassiana had the highest dry root weight and tuber yield....
Iftikhar Jan* and Sahib Alam
...genic strains for use as biocontrol agent. A total of 434 isolates of A. flavus were isolated from soil and stored maize grain samples collected from four agroecological zones (AEZ) i.e., AEZ-A, AEZ-B, AEZ-C and AEZ-D of Khyber Pakhtunkhwa (KP) province of Pakistan. The mycological analysis of the samples demonstrated that the highest population of Aspergillus section Flavi was existed in soil (n = 236) than in maize grain samples (n = 198). Across all the fou...
Azra Nadeem1*, Shaukat Hussain2, Saeedullah2, Asma Akbar3, Zahoor Ahmad4, Maryam Tariq5, Robina Karim6 and Muhammad Huzaifa7
...ut Trichoderma viride as biocontrol agent in controlling black scurf was investigated under controlled conditions. Data were recorded for disease severity, incidence, tuber weight, number of tubers/plant, fresh root weight and plant height at the time of harvest of the crop. The biocontrol agent (T. viride) showed lowest disease incidence (53.73%) and severity (1.27) at 8% moisture level. There was a reduction of 43.33% and ...
Samreen Khan*, Salma Javed, Tabassum Ara Khanum, Nasira Kazi
...in captioned district as biocontrol agents against insect pests of agricultural systems. One of the alternative method against insecticides for better management of plant health under greenhouse and field circumstances in integrated pest management program. Being potential of these nematodes as biopesticides may be distributed to farmers to reduce use of insecticides already in practice and to control hazardous effect of chemical pesticides on both crops and h...
Muhammad Asif1*, Ahmad Ali Shahid1,2 and Nasir Ahmad3
... lucidum is an efficient biocontrol agent to reduce the disease incidence. Crude protein extract was also found beneficial to control the pathogen. The results for minimum inhibitory concentration (MIC) of crude protein extract reveal that 5mg/ml is the concentration, which inhibited 100% growth of A. solani. It was concluded that either mycelia or crude protein extract of G. lucidum could be used for pathogen inhibition.
...
Rida Haroon Durrani1, Ali Ahmad Sheikh1*, Masood Rabbani1, Muti-ur-Rehman Khan2, Aneela Zameer Durrani3, Salman Ashraf1, Muhammad Anas Naeem1, Muhammad Usman3 and Tauqeer Mahmood4
...is promising pre-harvest biocontrol approach to curtail host-adaptive antibiotic resistant Salmonella. ...
Kanwal Hanif1, Dilbar Hussain1, Qurban Ali1, Asad Aslam1*, Tamsila Nazir1, Muhammad Saleem1, Muhammad Faheem Akhtar1, Muhammad Zubair2, Najuf Awais Anjum3 and Muhammad Umar Qasim1
... wasps that are powerful biocontrol agents against a variety of insect pests. These biological control agents retain their importance due to their easy mass rearing, high searching ability and effectiveness against many crop insect pests. In this study the toxicological effect of seven insecticides viz Flubendiamide (Belt 480 SC)@ 500 ul/l, Pyriproxyfen (Priority 10.8 EC) @ 2000 µl/l, Chlorantraniliprole + Thiamethoxam (Voliam Flexi 300 SC) @ 800µl...
Shahid Iqbal, Muhammad Aslam Khan, Muhammad Atiq*, Nasir Ahmed Rajput, Muhammad Usman, Ahmad Nawaz, Ghalib Ayaz Kachelo, Azeem Akram and Hadeed Ahmad
...uch as chemical control, biocontrol, use of Phyto-extracts and nanotechnological approaches have been introduced to combat this disease. The synthetic fungicides are used to curb the disease incidence. Pathogen have developed resistance against various chemicals that are generally utilized to overcome this disease. Because of its antifungal potential against mango anthracnose, among these strategies, nanotechnology is a rapidly evolving discipline that is gain...
V. Sunitha*, V. Vasudeva Rao, V. Ravinder Reddy, P. Venkateshwarlu, A.V.L.N. Ramalingeswar Rao and B. Naresh
A.H. Choshali1, S. Rezaee2, S. Jamali3, H. Reza3, Zamanizadeh4 and F. Rejali5
...as a suitable option for biocontrol of the root-knot nematodes....
L. Yue, S. Ahmed†, Q. Liu and H. Jian
... justify;">The effect of biocontrol agents comprising Bacillus sp., and Streptomyces rubrogriseus on the management of the root-knot nematode Meloidogyne icognita was investigated along with a nematicide Fosthiazate on tomato susceptible cultivar cv. Baiguoqiangfeng. The S. rubrogriseus culture filtrates (CF) showed maximum mortality of second stage juvenile (J2) followed by Bacillus sp., at 12 h and 24 h duration of exposure time as compared to the control tr...
I.K.A. Ibrahim1 and Z. A. Handoo2†
...leurotus ostreatus), the biocontrol agent Bacillus thuringiensis, the bionematicide abamectin, and the nematicide fenamiphos on Mi 1 on rice cv. Sakha 101. All the applied control treatments were effective in reducing nematode infection on rice plants....
R.R. Arain, R.N. Syed, A.Q. Rajput, M.A. Khanzada1, N.A. Rajput and A.M. Lodhi1†
...ed that incorporation of biocontrol agent like T. harzianum and botanical products
such as neem extract against root-knot nematode provided good results and can provide an alternate of chemical
pesticides which will be ecofriendly as well.
...
Hina Safdar1,2*, Nazir Javed2, Sajid Aleem Khan2, Muhammad Zeeshan Majeed3, Arif Mehmood3 and Muhammad Arshad4
...des (EPNs) are effective biocontrol agents against different insect pests. However, very little work has been done in Pakistan on the symbiotic bacteria associated with EPNs and their metabolites. In this laboratory study, two bacterial isolates i.e. Xenorhabdus spp. associated with EPN Steinernema glaseri (Steiner) (Steinernematidae) and Photorhabdus spp. associated with EPN Heterorhabditis bacteriophora (Poinar) (Heterorhabditidae), were evaluated against ar...
A. N. Mahar, N. D. Jan†, A. Q. Mahar and S. R. Gowen
Muhammad Rizwan1*, Bilal Atta1, Muhammad Arshad2, Sohail Akhter3,
Muhammad Tahir3, Misbah Rizwan1, Muddassir Ali1 and Arshad Makhdoon Sabir1
...tempunctata as a natural biocontrol agent....
Muhammad Nadeem1, Jamshaid Iqbal2, Tariq Mustafa3, Gul Rehman2, Muhammad Faisal Shahzad2, Muhammad Younas4,5*, Aftab Ahmad Khan6, Ameer Hamza2, Abdul Ghaffar1 and Muneer Abbas1
...F) are currently used as biocontrol agents and are alternatives of synthetic insecticides in sustainable agriculture. The bio-efficacy of entomopathogenic fungi (EPF); Metarhizium anisopliae (PacerMA), Verticillium lecanii (Zimm) (Mealikil-VL), Isaria fumosorosea (Wise) and Beauveria bassiana (Bals) (Racer-BB) were investigated against the mung bean thrips, Megalurothrips distalis Karny (Thysanoptera: Thripidae). Evaluations were based on thrips population, pe...
Mahmoud Mohamed Ahmed Youssef and Wafaa Mohamed Abd - El-Hameed El-Nagdi
Mahmoud M.A. Youssef1, Wafaa M.A. El-Nagdi1*, Hassan Abd- El-Khair2, Usama S. Elkelany1, Mahfouz M.M. Abd-Elgawad1 and Mona G. Dawood3
Sukhpreet Kaur Sidhu1*, Gurkirat Singh Sekhon1, Sachin Kumar1, Tejdeep Kaur Kler1 and Anureet Kaur Chandi2
...d ecological services as biocontrol agents corresponding to Rabi and Kharif crops. Results have shown that foraging frequency of black drongo (Dicrurus macrocercus) was the highest followed by green bee-eater (Merops orientalis), wire-tailed swallow (Hirundo smithii), common swallow (Hirundo rustica) and house swift (Apus affinis). Study results have highlighted the biocontrol potential of insectivore avian species along wit...
Aalaa S. Saad1*, Taghreed H. A. Ali2, Nayerah Alatfeehy3, Dalia Elmasry4
...at natural product-based biocontrol techniques have the potential to be developed to control the development of L. monocytogenes in chicken and chicken products. This result indicates that using natural and bio-based products can effectively manage diseases in the food industry. ...
*AMNA SHOAIB, AROOJ SHEZAD, ARSHAD JAVAID, SUNDUS AKHTAR & ZOIA ARSHAD AWAN
