Qaizar Ahmed1, Fida Mohammad2, Hidayat-ur-Rahman2, Sheraz Ahmed2* and Fakharuddin2

...or various agronomic and biochemical traits in Flue Cured Virginia tobacco. Seven tobacco varieties/lines, ‘NC606’, ‘K399’, ‘Spt G 126’, ‘Spt G 28’, ‘KHG21’, ‘KHG22’ and ‘KHG24’ with contrasting traits were crossed in all possible combinations to generate 7 x 7 diallel crosses. In 2008 and 2009, all F1 hybrids along with their parent cultivars were planted in randomi...

Wan-Long Zhu* and Gao Wenrong 

...iological, hormonal, and biochemical markers indicative of thermogenic capacity. The results showed that A. chevrieri adapt to winter or under cold condition by increasing thermogenesis and food intake. The present results suggest that the observed physiological regulation from the organismal, hormonal levels to the cellular levels of this alpine small mammal were important and allowed A. chevrieri to overcome the physiological challenges of an c...

Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa

...rized using conventional biochemical tests and polymerase chain reaction (PCR), respectively. Six (6) were observed to ferment glucose, reduce tetrazolium chloride and hydrolysed casein. They had no phosphatase activity, did not produce ‘film and spots’ and neither hydrolysed arginine nor urea and were preliminarily identified as members of the Mycoplasma mycoides sub-cluster which comprises Mmm and Mycoplasma mycoides subspecies capri (Mmc). Three...

Muhammad Mushtaq, Umer Sadique, Naila Chand, Iftikhar Ahmed, Said Sajjad Ali Shah, Ijaz Ahmad, Imran Ullah and Muqadar Shah

Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1

...stimated. Moreover these biochemical parameters were confirmed by histopathological examination using hemotoxylin and eosin (H&E) and Mason trichrome stains (MTC). Immunohistochemical investigations for the expression of the proapoptotic protein (Bax) and the expression of macrosialin cluster of differentiation (CD68). APAP elevated of most of the previously measured parameters and decreased GSH, SOD, and total protein levels. Liver sections of H&E dem...

Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*

...ection. Histological and biochemical investigations were performed.Live P. chabaudi infection was accompanied with an increase in spleen weight in comparison to either the control or the gamma irradiated P. chabaudi infected group. Differential count of WBCs has revealed an increase in lymphocytes, monocytes and granulocytes count in plasma samples of the live P. chabaudi infected group in comparison to either the control or the gamma irradiated P. chabaudi in...

Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*

...es. Their morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. Their sensitivity against three phages indicated broad host range of phages SANF and SA2, and relatively narrow host range of phage SA. Phage SNAF showed an effective growth reduction of S. aureus RP isolate, compared with other bacteriophages. The bacterial challenge test in ...

Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1

...gical, physiological and biochemical characterization. 16SrRNA sequencing was performed to identify three highly resistant strains at species level. Antimicrobial activity of aqueous and methanolic plant extracts of Camellia sinensis (Green tea), Syzygium aromaticum (Clove) and Mentha piperita (Peppermint) was evaluated against identified isolates. Agar well diffusion assay was used to monitor the antimicrobial activity of these strains both in mono culture an...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...is test and furthered to biochemical analysis. Pearson’ Chi-square test at nighty five percent confidence interval was used to analyze the collected data. Antibiotic sensitivity was checked with twenty four antibiotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67%, 50/120) form of mastitis on overall milk collected data. Pathogen type was crowned by Staph. aureus with 74.5% (47/63) prevalence...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
..., hematological and bile biochemical response in buffaloes to Gigantocotyle explanatum infection. Of the 558 slaughtered buffaloes from central Punjab, Pakistan, 18.99% were infected, and morphometric analyses were made of 115 mature amphistomes and gravid eggs with traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmine, some were sectioned in the median sagittal plane and histo...
Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3
...of histopathological and biochemical values. In conclusion, EA might play an important role in repairing D-GaIN-induced liver damage both as a protective and a therapeutic agent.
...

A. K. Ganguly and Uma Rao

Decades of researches in biochemical and molecular nematology at IARI
...main research areas were biochemical mechanism of resistance against phytonematodes and diagnostics of economically important plant parasitic nematodes. Specific isozymes have been identified for differentiating the important root knot nematodes species of India belonging to the genus Meloiodogyne. Further, molecular markers like RAPDs, AFLP, SSRs and PCR–RFLP of rDNA have been employed to study the molecular genetic diversity in major root knot and cyst...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...conducted to compare the biochemical and histopathological changes in response to pathogenic field infectious bursal disease virus (IBDV). The Bursa of fabricius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived anti...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...mic resources for future biochemical, toxicological and physiological studies in common carp.
...
Mutassim M. Abdelrahman1,*, Riyadh S. Aljumaah1, Moez Ayadi1 and Shabana Naz2
...ion. Colostrum yield and biochemical analysis at parturition was also determined. A significant (P<0.05) increase of calcium (Ca), Zn, Co and Se levels in blood of ewes treated with bolus with a significant increase in phosphorous (P), Co and Se in the blood of their newborn lambs. Furthermore, a significantly higher (P<0.05) inorganic matter percentage in colostrum was found in treated group compared to the control. The body weight of lambs in treated g...
Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
... hematological and serum biochemical parameters were determined to establish the reference values in the wild Indian blue peafowl (Pavo cristatus) of Thar (desert) region in the Sindh province of Pakistan. A total of 60 blood samples were collected from peafowl (30 males and 30 female) in months of March and April, 2015. The gender difference showed that RBC, MCV and MCH concentrations were significantly higher (P < 0.05) and the serum total protein ...
Mutassim M. Abdelrahman1*,Ibrahim Alhidary1, Abdullah H. Alyemni2, Rifat U. Khan3, Abdel Raouf S. Bello4, Mohamed Y. Al-Saiady2 and Ramzi A. Amran1
Kulsoom Akhter1, Tahseen Ghous1,2,Saiqa Andleeb3,*, Faiz-ul-Hassan Nasim4, Samina Ejaz5, Zain-ul-Abdin1, Bilal Ahmed Khan1 and Muhammed Naeem Ahmed1
... basis of morphological, biochemical, 16S rRNA gene sequencing, the isolates were identified as B. cereus BDBC01, B. cereus AVP12 and B. cereus NC7401. At maximum studied concentration of metal ions (250 mgl-1), in above sequence, the strains accumulated 118.2, 121.87 and 90 mg/g Cr (VI), 135, 127.5 and 146.25 mg/g Ni (II) and 120, 129.37, 135 mg/g Cd(II). Langmuir and Freundlich models were applied and found suitable to describ...
Nargis Bano and Khalid Mahmood Qureshi*
..., along with various and biochemical attributes, but augmented the leaf proline. SA application significantly increased plant fresh and dry weight, leaf area, proline and decreased root length, number of roots and water use efficiency. SA treatment therefore decreased adverse effect of stress on strawberry plants. Thus the result suggests that SA at a concentration of 3mML-1 concentration could be used commercially to improve yield of stra...
Mehmet Guvenc1,*, Ayşe Nilay Guvenc3, Ökkeş Yılmaz2 and Mehmet Tuzcu2
... a bioregulator for some biochemical parameters in fructose induced non-alcoholic fatty liver.
...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*

 

... agar and identified via biochemical tests. Polymerase chain reaction was used to detect representative virulence factors or genes, including E.coli adherence factor (K88, CS31A, afaE-8), toxins (estA, estB, Stxl, Stx2, EAST1), pathogenicity island (eaeA, irp2, ETT2) and outer membrane protein (ompA). Moreover O-antigen serotype was tested by slide agglutination test and a mouse model was built to assess the lethality ...
Aqsa Imtiaz and Abdul Rehman*
...us subtilis based on biochemical characterization and 16S rRNA Ribotyping. B. subtilis BML5 showed maximal keratinase activity at 37°C and at pH 8. An increase in keratinase activity of B. subtilis BML5 was determined in the presence of Ca2+, Mg2+, and Zn2+ while keratinase activity was inhibited by added copper ions. A 130-kDa protein was detected from feather-treated bacterial cells, sugges...

Irfan1* , Arshad Javid2 , Muhammad Altaf3 , Muhammad Shahbaz3 , and Khalid Javed Iqbal4 

...t;;">Variations in serum biochemical profile with increase in age were analyzed in turkeys (Meleagris gallopavo). Genderwise variations and effect of rearing systems i.e. free range, semi-intensive and confinements were also assessed from 1st to 6th month of age. Total sixty (n = 60) experimental birds were divided in to three groups i.e., poults having age 4-8 weeks (10 ♂...

Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2

 

...symptoms and till now no biochemical test is available for its diagnosis. It is considered one of the most costlydisease in terms of diagnosis and treatment. Its heritability content is considered to be up to 80% but still it isunknown whether it’s a pure genetic disease. It is considered a polygenic multifactorial condition where both genes and environment have roles in disease development. In the present study we selected a family withmultiple schizoph...

 Asma Akhtar, Abdul Rehman

...s lactis on the basis of biochemical tests and 16S rRNA sequence. The minimum inhibitory concentration of both isolates showed fair growth up to 1 mM of K2Te2O3. Both strains showed maximum growth at 37ºC and pH of 7. The bacterial growth in the presence of metal ions was delayed as compared to the control. High ellurite reduction potential was shown 98% (1f) and 97% (2b) after 24 h of incubation. Glucose 6 phosphate dehydrogenase (G6PDH) activity was inc...
Rabia Qurashi, Qurat ul Ane Gillani* and Furhan Iqbal*
 
... hematological and serum biochemical profile of 6 week old male albino mice. Blood samples from male albino mice (CGP55845 treated N = 14 and saline treated N = 16) were collected directly by cardiac puncture and used for estimation of hematological and some serum biochemical parameters. The hematological parameters did not show any significant variation between the two groups. From amongst serum bio...
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
...tive in regulating other biochemical parameters. However, significant role of garlic extracts; especially the ether extract (EGE) was noted in normalizing the biochemical attributes. On the basis of findings, it is recommended that the garlic ether extract should be applied in herb-drug combinations commonly used as antidiabetic.
...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
Samra Imran1,* and Afifa Tanweer2
... through anthropometric, biochemical and dietary evaluation of the participants. Results indicated that participants aged less than 18 years and those diagnosed within first year of life, had significantly healthier body dimensions. Higher family income, female gender and greater meal satisfaction was found to be associated with better biochemical indices. Most participants reported partial compliance to GFD. Compliant parti...

 Abdullah Adil Ansari* and Kumar Sukhraj**

...ificant influence on the biochemical characteristics of the soil with marked improvement in soil micronutrients. The combination treatment [VW+VC] was found better suggesting qualitative improvement in the physical and chemical properties of the soil, which is substantiated by ANOVA and composite index {Rank 1 for [VW+VC] with composite index of 9}. This biological method of crop cultivation is sustainable and improves soil health rather than conventional meth...

 Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan*

...ve and quantitative) and biochemical trait. Comparatively high variation was seen for seed germination, canopy and spikes/plant, florets/spike and leaf area. A UPGMA cluster, grouped the 9 Ocimum genotypes into two major clusters on the basis of total seed protein and phenotypic characters, except Siam Queen and Lime basil stand alone, respectively. Euclidian distance for morphological traits ranged from 3.60 to 7.26. Conversely on the basis of total seed prot...
Muhammad Kashif Maan1, Muhammad Arif Khan1, Shehla Gul Bokhari1, Muhammad Ijaz2, Hamid Akbar1, Sajid Umar 3 and Muhammad Luqman Sohail4,*
...omy.
...

 Muhammad Zahid Kiani*, Arshad Ali**, Tariq Sultan** and Muhammad Munir Ahmed***

CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM ROOT SYSTEM OF SUNFLOWER (HELIANTHUS ANNUS L) GROWN UNDER SALT AFFECTED AREA OF PAKISTAN
...rphologically as well as biochemically at National Agricultural Research Centre, Islamabad during 2011-13. It was observed that all isolates produced IAA, whereas14 strains were declared as phosphate solubilizing bacteria (PSB), eight isolates exhibited antifungal characteristics, 30 were nitrogen fixer and all of them were gram -ve. During biochemical characterization of bacterial isolates KS 15 and KS 8 produced the highes...

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...and centrifuged prior to biochemical analyses. Results showed differences in the activities of enzymes and quantities of total protein, soluble protein and FAA between strains and among concentrations. Abamectin, at LC and LC , changed the activities 10 20 /levels of TE, CE, AcP, total protein and FAA in the larvae of both the strains. The activities of alpha-amylase, glucoamylase and AkP remained non-significant at both doses in the two strains. In PAK strain...
Alaeldein M. Abudabos*, Abdullah N. Al-Owaimer, Elsayed O.S. Hussein and Mutahar H. Ali
Wei Dang1, Ning Xu1, Wen Zhang1, Jing Gao2, Handong Fan1 and Hongliang Lu 1,*
...ecific physiological and biochemical adaptations. Heat shock proteins and other molecular chaperones play specific physiological roles in such thermal adaptation. Here, we analyzed heat shock protein 70 (Hsp70) expression in six lizard species to investigate the variation in Hsp70 response contributing to thermal adaptation. At first, we collected three lizard species of the genus Takydromus from different geographical locations. We found that either th...

Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4 

... pulse rate (PR) whereas biochemical parameters determined from blood serum were glucose, cortisol and Heat shock protein-70 (HSP-70). Thermal stress increased all physiological parameterssignificantly (P < 0.001). Holstein Frisian manifested maximumincrease in RT, RR and PR (3.33, 209.50 and 59.41%) than crossbred (0.59, 40.22 and 22.0%), Sahiwal (0.78, 42.54 and 34.31%) and Achai (0.78, 39.22 and 33.85%) respectively (P < 0.001).Thermal stress increase...

Nadia Mubarik1, Aqib Iqbal1*, Iqbal Munir1 and Muhammad Arif2 

... the water relations and biochemical adaptation, resulting in improvement in the performance of plants under water deficit conditions. These results also demonstrated that senescence, which is a type of cell death program that could be inappropriately activated during drought, was also delayed in the supplemented seedlings as highlighted by a high RWC and expression of the Lhcb2 gene. 

...
Naimat Ullah1,3,*, Aneela Zameer Durrani1, Muhammad Avais1, Nisar Ahmad2, Sana Ullah3, Muhammad Shuaib Khan3, Khalid Mehmood5, Mumtaz Ali Khan1 and Ikramul Haq1
Syeda Madiha, Zehra Batool, Saiqa Tabassum, Laraib Liaquat, Sadia Sadir, Tahira Perveen and Saida Haider*

 

...-induced motor deficits, biochemical and neurochemical alterations. Rotenone was injected subcutaneously at the dose of 1.5 mg/kg for 8 days. Supplementation of curcumin (100 mg/kg/day, p.o.) was started before 15 days of rotenone injection. The effects of curcumin pre-treatment were evaluated by different motor behavioral parameters (pole test, Kondziela’s inverted screen test, inclined plane test, open field test, beam walking test and footprint test)....
Hamayun Khan1,*, Abdul Wahab1, Navaid Kazi2, Younas Muhammad1,Summaya Kazi3, Salman Kazi4, Azmatullah Khan1, Ikramullah Khan5 and Shakoor Ahmad1
...this study was to assess biochemical markers of rabbit amniotic fluid during fetal growth stage of successful pregnancy. Experiments were executed on 15 pregnant rabbits at three different time point of successful pregnancy i.e. day 18, day 25 and day 28 with commercially accessible kits for biochemical markers that includes glucose, total protein, albumin, creatinine, triglycerides, blood urea nitrogen (BUN), alanine...

Sher Nawab Khan* and Ghulam Hassan 

...or further molecular and biochemical study to explore resistance pathway. These lines could be used as a source of aphids resistance in future wheat breeding program 

...

Muhammad Suhaib1*, Ijaz Ahmad2, Masooma Munir3, Badar-Uz-Zaman1, Bushra Atta4 and Muhammad Khubaib Abuzar5 

...ts on the physiology and biochemical parameters of crop plants however lower level of salicylic acid 0.25 mM was found more efficient then the higher level of salicylic acid. Salicylic acid induced improvement is significant regardless of stress environment or normal growth conditions.
 

...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...riculture farmlands. The biochemical and physiological characterization along with the molecular identification by 16S rRNA gene sequencing confirmed that caries isolates are pathogenic S. mutans and soil isolates belong to the genus Streptomyces. The antibiotic resistance profile of S. mutans illustrated resistance to optochin. The antimicrobial screening of Streptomyces against S. mutans showed that these strain are the pro...
Huma Naz1,*, Sajid Abdullah2, Sidra Naz3, Sidra Abbas2, Wardah Hassan2, Shakeela Perveen2, Moazama Batool1, Laiba Shafique2
... that the behavioral and biochemical parameters can be used as a valuable bio-marker to identify the toxic effects of pollutants in aquatic systems. 
...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...as aimed to evaluate the biochemical effects of λ-cyhalothrin on carbohydrate metabolism and macromolecular concentrations of 4th and 6th instar larvae of a stored product pest, Trogoderma granarium. The LC50 values of λ-cyhalothrin for 4th and 6th instar larvae of Lahore population was 15.93 and 13.76ppm respectively, while 19.07 and 16.21ppm were for the 4th and 6th
Muhammad Nazar Aftab1, Ahmed Ali1, Muhammad Asad1, Sadia Fatima2 and Furhan Iqbal2,*
...ed toxicity on the hematobiochemical profile of male albino mice. Eight week old male albino mice were used as experimental animals and were divided into two groups. First group was treated with 80mg/Kg body weight of AlCl3 for 16 days while control group was treated with saline solution for the same period of time. Complete blood count were determined in both experimental treatments at the end of dose supplementation. Our results revealed that oral...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
...cters, Gram staining and biochemical tests. The identified isolates were quantified on blood agar and confirmed through PCR. Toxins were extracted, quantified, formalized and adjuvanted with aluminium hydroxide gel. Safety, sterility and stability of the toxoid were ascertained. Bacterin toxoid of C. perfringens type D was procured from Veterinary Research Institute Peshawar. The immunogenicity of toxoid vaccines and bacterin toxoid vaccine were studied...
Imran Taj1,*, Ferhat Abbas1, Zafar Ahmad1, Mohammad Kamran Taj1, Deedar Ahmad2, Zahoor Ahmed2, Ghulam Mohammad2, Ajaz-ul-Haq1, Farooq Shahzad1 and Sabiha Azam3
...tified through different biochemical tests and PCR. Antibiograms and lab animal trials were also carried out on local isolates of C. perfringens type D. Out of 204 analyzed samples, 89.18 % were found positive while 10.82 % were negative for C. perfringens type D. The division wise distribution showed that Quetta, Zhob, Sibi and Kalat were more effected zones of the Balochistan province. Sheep were more effected (54.78 %) than goats (34.4 %). ...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
... days. Blood samples for biochemical analysis (blood glucose, cholesterol and total protein) and gills for histology were taken after 96 hrs. After acute exposure to pesticide, significant changes were observed in serum biochemistry and histology. Serum glucose and cholesterol were increased while total protein was decreased. Histopathological result reveled that gills of experimental fish was damage severely resulting Necrosis, Damaged nuclei , rupturing of e...

Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil

Nimra Naseer, Adeela Fatima and Imran Sajid*
...creened biologically and biochemically for their potential bioactive secondary metabolites against MDR bacterial pathogens. The identification of strains was done by morphological, physiological and biochemical characterization and by 16SrRNA gene sequencing. For biological screening different methods including cross streak, agar plug and well diffusion method were performed. Seven Streptomyces strains including NWMD-...
Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...talyzed by NOS. Based on biochemical analysis of the above, results showed that O2- production could be induced significantly after cold stress at 15°C and heat stress above 30°C. SOD activity showed a similar changing profile as the concentration of O2- after thermal stress between 15°C and 30°C, and GSH concentration increased significantly under both high and low temperature. The NOS activity and c...
İnan Kaya1,*, Muhitdin Yılmaz2, Müge Mavioğlu Kaya3,Abdulsamed Kükürt4 and Mahmut Karapehlivan5
... taken from the fish for biochemical studies at the end of the study period. Oxidative stress index (OSI) in plasma were evaluated as well as AST and ALT levels in the liver. Plasma OSI and liver AST levels in the groups with carbaryl and 2,4-D were compared with the control group, and they were found to be significantly higher. As a result, it was concluded that carbaryl and 2,4-D administered under of LC50 value caused liver damage by increasing t...
Farah Mansha, Abdul Rehman*
... sp. on the basis of biochemical tests, and showed optimum growth at 37°C and pH of 7. All the bacterial isolates showed highest enzyme activity at pH 7. Isolates H4 and M4 showed maximum enzyme activity at 55°C while H1 showed highest activity at 70°C. Chitinase activities exhibited by H1 and M4 are stimulated by the presence of Zn2+ ions, while the presence of Ni2+,Cd2+ and Cu2+ did not show any pos...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
...ferent morphological and biochemical tests were performed to identify and characterize bacterial pathogens. Among isolated strains, two belong to genus Staphylococcus spp., other two were Pseudomonas spp. and one strain belonged to genus Salmonella spp. Effect of various parameters such as temperature and pH on growth of pathogens were also studied. Antibiogram and resistogram analysis were carried out through agar disc and agar well diffusion methods. Optimum...

 Sumera Siddique1,†, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1*, Amtul Bari Tabinda2, Muhammad Irfan1

Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
...sults indicate important biochemical attributes of the abundantly produced fruit wastes and their efficiency for economic production of C. tropicalis SS1 which could be used as SCP supplementing animal feed’s formulations.

...

 Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2

Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...idney were collected for biochemical and histopathological analysis.Cr+6 treated group showed severe histological changes like necrosis, cirrhosis and dehydration in liver evident by significant elevation of Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT), Alkaline Phosphatase (ALP), total protein, bilirubin, globulin, creatine and uric acid along with reduction of SGOT/SGPT, Blood Urea Nitrogen (BUN), urea and albumi...

Riffat Mehboob1*, Sami Ullah Mumtaz2, Zoya Manzoor3, Sajid Abaidullah2, Fridoon Jawad Ahmad1

Deranged biochemical and hematological profile of septicemia patients in Mayo hospital, Lahore

 Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3

Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...as selected to study the biochemical and histological
alterations in liver of silver carp. The LC50 1.6 μg/L was determined. Fish (n=6) were dividing into 4 groups A (control)
B, C and D (experimental). Experimental groups B, C and D were exposed to 25%, 50% and 75% of LC50
respectively for acute exposure. The blood and liver tissue sampling was done after 96hrs for histology and
biochemical an...

Dilara Abbas Bukhari1, Abdul Rehman2*

Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus
Haniya Mazhar1, Naaz Abbas2, Tehseen Zamir3, Zahid Hussain1, Syed Shahid Ali4*
...following morphological, biochemical and molecular characterization. Best medium for lipase production was olive oil and mustard oil cake and the best carbon sources were fructose and maltose whereas yeast extract was found to be the best nitrogen source, showing 55U/mL enzyme activity. The best inoculum size for the growth of this strain was 4-5%. Optimum pH was 8 and best temperature was 40oC for the reported strain. Addition of divalent Mg2+...
A.M. Abdul Azeem, A.N. El Shahat* and Ashraf M. Mounir
...ls extract (GPE) against biochemical alterations of lead induced testicular and hepato-toxicity in rats. From the results, it was obtained that gamma irradiation induced significant increase in the total phenolic content and antioxidant activity of pomegranate peels powder. Concurrent treatments of lead-injected rats with GPE induced significant reduction in serum activities of liver enzymes, level of hepatic and testicular lipid peroxidation and plasma levels...
Muhammad Ijaz1, Shahid Hussain Farooqi1, Rahmatullah2, Amjad Islam Aqib1, Sadaqat Ali3,*, Awais Ghaffar1, Ahmad Ali1 and Sehrish Saleem1
...tocrit and various serum biochemical parameters (serum electrolytes, trace and ultra-trace elements) in foals. A total of 105 diarrheic foals (n = 35 horse foals, n = 35 donkey foals, and n = 35 mule foals) were selected regardless of their etiologies. Additionally, 12 healthy foals (n = 4 horse foals, n = 4 donkey foals, and n = 4 mule foals) as control animals were selected for this study. Packed cell volume (PCV) and serum electrolytes, trace and ultra-trac...

Fouzia Tabssum1, Qamar uz Zaman2*, Yinglong Chen3,4, Umair Riaz5*, Waqas Ashraf6, Ambreen Aslam2, Nusrat Ehsan2, Rab Nawaz2, Humera Aziz7 and Shamim ul Sibtain Shah8 

...nd water potential), and biochemical attributes (SOD, CAT, MDA, EL and T CHL). Under field conditions, exogenous application of optimized dose of proline both at seedling establishment and vegetative stage significantly improved the performance of rice cultivars by improving tillering dynamics, plant water-relations, chlorophyll pigments, photosynthetic pigments, morphological and yield related traits of rice cultivars. Proline application improved the efficie...
Tamoor Azeem1,*, M. Yasin Tipu1, Asim Aslam1, Sajjad Ahmed2, Salman Ahmed Abid1, Abdullah Iqbal3, Naeem Akhtar4, Muhammad Saleem4, Aamerzish Mushtaq4 and Sajid Umar4
...ed to investigate haematobiochemical alterations in camels during haemoparasitism. Blood samples were collected from 200 camels of either sex and different ages from district Attock. Samples were processed for haematobiochemical analysis and thin smear stained with Field stain according to standard procedure. Out of 200 camels 46% of them have blood parasites. Trypanosoma was the most prevalent (32.5%) followed by dip...
Tafail Akbar Mughal1, Muhammad Zubair Saleem2, Shaukat Ali3,*, Khawaja Khurshid Anwar1, Muhammad Majid Bashir1, Muhammad Babar4 and Muhammad Adeeb Khan1
...ctate dehydrogenase) and biochemical components like glucose, urea, lipids, cholesterol and protein contents both in the liver and blood while DNA and RNA contents only in liver. The administration of CCl4 resulted in increase in plasma ALAT and decrease in LDH. Vitamin E pre-treatment abolished CCl4-induced changes in the activities of these enzymes. Blood glucose content was increased while cholesterol content was decreased. Vitamin E p...
Fariha Latif* and Muhammad Javed
...div>The potential use of biochemical techniques along with antioxidant parameters has gained considerable attention due to their use as biomarkers to assess contaminants impact on fish at earlier level of exposure. Fish from different species has diverse ecological needs and respond differently to the environmental stress conditions. Thus, the sensitivity, bioaccumulation and antioxidant capacities of three major carps Catla catla, Labeo rohita a...
Muhammad Usman Ahmadand Ikram-ul-Haq*
... basis of microscopy and biochemical testing. E. coli strain EC4 was screened as best oxyrase producer with activity of 0.41±0.008U/mL/min with 41% reduction in dissolved oxygen at pH 7.5, temperature 37°C, 25mM lactate as H+ donor after 20 min. This strain was later on confirmed by 16S ribotyping procedure. Oxyrase was used for improved cultivation of anaerobic bacteria by employing Hungate Technique where its antioxidant potentia...
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
...ological, physiological, biochemical, molecular and genetic characteristics of the plants. As the information about the relationship between physico-morphic characteristics of commercially grown okra cultivars and resistance or susceptibility to Helicoverpa armigera is lacking, therefore, in the present study relationship between physico-morphic characteristics and resistance or susceptibility of okra cultivars to H. armigera was evaluated. The h...
Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu
...ith these measured serum biochemical indexes except for LDL-C. Significant additive effects were detected for all detected serum biochemical indexes except for LDL-C between allele A and G, and significant dominance effects for TP. These findings suggested that ChREBP might be a crucial candidate genes for the selection of duck lipid content.
...
Shehzad Ghayyur1,3, Sadia Tabassum1, Munawar Saleem Ahmad2Naveed Akhtar1 and Muhammad Fiaz Khan1,*
... hematological and seral biochemical components of Oreochromis mossambicus. RBCs, Hb and Hct decrease significantly (p < 0.05) indicating an anemic condition, while the WBCs and platelet counts increased significantly (p < 0.05) in all the treated groups compared to the control group. Among the seral biochemical parameters, the level of blood glucose, cortisol and cholesterol were significantly increased (p < 0.05),...
Bibi Nazia Murtaza1,2, Azhar Qayum3, Shamaila Inayat Nadeem1, Naif Awdh Al-Maliki4, Abdulaziz Alamri4 and Abdul Rauf Shakoori2,5,*
...ansformative capacity by biochemical and signalling readouts in these patients. Dynamical implications and functional impact of variants were determined by in silico analysis and molecular docking of variants with GTP. MutationTaster was used for functional analysis of genetic variants and three-dimensional structure of mutant proteins were built by Swiss-Model and were further subjected to structural alignment and stability studies by I-Mutant Suite and DUET ...

Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1 

...ght microscopy and other biochemical tests. The bacteria were tentatively identified as Pseudomonas, Streptomyces, Bacillus and Micrococus. These bacteria were checked for their antagonistic activity against Fusarium oxysporum f.sp. alium cepa. They all were found to inhibit fungus showing inhibition zone 4.09 -74.97%. Hence, on the basis of these studies, it can be concluded that these bacteria can be used as a biocontrol agent to manage other fungal diseases...

Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3 

...le was confirmed through biochemical testing. The quarter wise prevalence of subclinical mastitis was 25%. Within this, contribution of Gram-negative bacteria was 68% and that of Gram-positive bacteria was 32%. Among 30 positive samples, percentage prevalence of different bacterial species was: E.coli (37%), S. aureus (23%), Pseudomonas (20%), Streptococcus (10%), Proteus (7%) and Salmonella (3%). The study reported high percentage of E. coli in cases of subcl...
Yuyu Wang, Gangchun Xu*, Zhijuan Nie, Quanjie Li, Nailin Shao and Pao Xu*
...rowth performance, serum biochemical parameters, digestive enzymes activity and antioxidant status of largemouth bass (Micropterus salmoides) reared in an in-pond raceway system (IPRS). Fish (initial average body weight: 35.68±2.12g) were randomly allotted to in-pond raceways (26.2m×5m×2.5m) stocked at two stocking densities (68 and 114 fish/m3, respectively). Fish were fed twice daily (08:00 and 17:00), and the daily ratio...
Syeda Shazia Bokhari*, Ghashia Qayyum, Uzma Rafi and Aisha Waheed Qurashi
...tified through different biochemical tests and morphological characterization. Bacterial strains were screened and those showing positive results for their adherence ability, autoaggregation and coaggregation response were used for the vermicomposting process. Both of these strains were inoculated for vermicomposting and whole of the setup was maintained for 60 days.The results showed that microbes were characterised and identified as Bacillus cereus an...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
...ned by morphological and biochemical study. Only one strain DUV1 was confirmed by 16S rRNA gene sequencing as D. vulgaris (accession number: KY698020). It showed significantly reduced biofilm formation (52%; p<0.05) by test tube method compared to liquid interface coverslip assay (35%) and congo red assay. D. vulgaris was treated with various concentrations (5, 40, 80 and 100µM) of salicylidene acylhydrazide and 5-15 µM of protease...
Zil-e-Huma1, 2, Abdul Malik Tareen3, Kaleem Ullah3, Tauseef M Asmat4,Abdul Samad4Asim Iqbal2, Mohammad Zahid Mustafa3*,Irshad Ahmad5 and Sadeeq ur Rahman6
...olonial characteristics, biochemical profile and specie specific PCR. Multiplex PCR was applied to differentiate among the five main diarrheagenic E. coli pathotypes (enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and enterohemorrhagic E. coli (EHEC). Results show that the virulent EAEC strain of E. coli was found to be the most p...
Constance Obiageli Ejilibe1, Helen O. Nwamba2, Ifeanyi Chinedu Atama3,*, Chiamaka Lynda Ani2, Ifeanyi Oscar Aguzie3, Josephine C. Madu3 and Christopher Didigwu Nwani3
...e study investigated the biochemical responses of Bufo regularis tadpoles exposed to two widely used agricultural pesticides, Butaforce® and Termex®. The Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total protein (TP) concentrations in homogenized muscle samples was determined by standard procedures. ALT, AST and ALP increased significantly in response to concentrations of...

Chukwuemeka Calistus Okolo1*, Ikenna Onyema Ezeh2, Chinelo Nnenna Uju3 and Nwakaego Ernestina Nweze1 

...nt. Parasitaemia, haematobiochemical, and oxidative stress markers were determined. At day 13 PS, there were no significant (p< 0.05) variations in mean parasitaemia of groups A, B and D. At day 16 PS, the parasites had cleared from the peripheral blood of rats in groups A and B, but remained detectable in group D. Although group D had significantly higher total erythrocyte count and haemoglobin concentration compared to groups A--C, and E, no significant v...

Veterinary Sciences: Research and Reviews

June

Vol. 5, Iss. 1, Pages 1-32

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