Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*

 

...earches in Taiwan. Here, bioassay, multiplex RT-PCR and multiplex real-time RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and simultaneous infections were common in field. Uneven distributions of both viroids also occurred in different tissues of citrus. Two multiplex molecular detection methods provide the understanding of the genetic diversities among viroid isolates and quantify viroids in citrus ...

Muhammad Zahid1, Muhammad Hamid Bashir1*, Bilal Saeed Khan1 and Muhammad Shahid2

...s were used and leaf dip bioassay was conducted. Pesticides were tested for their compatibility with N. barkeri at different concentrations under controlled laboratory conditions. The results showed 25%, 30%, 70%, 50% and 65% mortality occurred by tested pesticides at field relevant doses after 144 h respectively. Missing mites data indicated repellency due to pesticides, was highest at the start of experiment, and then these acclimatized on leaf discs. Thiacl...
Muhammad Afzal1,Naveeda Akhtar Qureshi1,*, Khalid Zamir Rasib2 and Irfan Hussain1
...were evaluated in choice bioassays in laboratory and field conditions. In force feeding field bioassays, least mass loss was recorded on D. sissoo (0.19±0.07g) and maximum on P. euramericana (1.50.1g). Under choice laboratory trials, maximum mass loss difference was recorded on pair of P. euramericana and D. sissoo (0.89±0.05 and 0.14±0.01g), whereas, minimum mass loss differe...

Dipak Mandal, Paramita Bhowmik, Pronobesh Halder and M. L. Chatterjee

Determination of relative toxicity and base line data of dif-ferent insecticides against cotton mealybug (Phenacoccus solenopsis Tinsley)
...gn: justify;">Laboratory bioassay was conducted to determine the toxicity of some insecticides and their LC50 values against 3rd instar nymphs of cotton mealybug, Phenacoccus solenopsis in the Department of Agricultural Entomology, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Mohanpur during 2012. Both treated food untreated insect and treated food treated insect methods were followed. The lowest LC50 values through treated food untreated insect method were re...
Ayesha Ilyas1, Hafiz Azhar Ali Khan2,* and Abdul Qadir1,*
...tration in a free choice bioassays. Acetone, chloroform, petroleum ether and ethanol were used for extraction from leaves. Amongst the various treatments applied, the acetone extract of D. alba showed the highest repellency of 84.14%, whereas, the lowest repellency of 10.73% was observed by the chloroform extract of T. peruviana. Significant ovipositional deterrent effects of the extracts applied have been observed. Maximum oviposition inhibition...

 Abdul Rehman Roonjho*, Waseem A. Gillani**, Awais Rasool**, Naheed Akhtar**, Tariq Mahmood**, Arsalan A.***, M. Afzal*, Iqbal Khan* , M. Asghar Ranjha**** , M. Irfan**** and Javed Khan**

REPELLENCY EFFECTS OF DIFFERENT PLANT EXTRACTS TO COTTON MEALY BUG, TINSLEY (HEMIPTERA: PSEUDOCOCCIDAE)
...bug ( ) in a free choice bioassay for two weeks. The ethanol extract of was the most effective against cotton mealy bug having highest repellency (72.5%) at 500 ppm dose. The lowest average repellency (26.3 %) was observed in acetone extract of at 500 ppm dose.

...
Hafiz Azhar Ali Khan1,*, Waseem Akram2, Sumi Lee3, Shakir Manzoor1, Syed Rajab Ayub1, Khalil Ur Rehman1, Shinawar Waseem Ali1, Muhammad Bilal Chattha1 and Sumaira Maqsood1
...d via diet incorporation bioassays. Based on median lethal concentration (LC50) values, all the strains of S. oryzae and R. dominica were more susceptible to spinosad than T. castaneum strains. The resistance ratios (RRs) of field strains at LC50 values were in the range of 2.24 to 3.24 fold for T. castaneum, 3.33 to 9.00 fold for R. dominica, and 1.73 to 3.45 fold for S. oryzae. The results revea...
Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
...asitism. In vitro bioassays were used to evaluate the efficacy of T. hamatum and difenoconazole alone or its combinations against S. sclerotiorum. The results showed that T. hamatum could infect and destroy the mycelia and sclerotia of S. sclerotiorum by mycoparasitism. The cell-free culture supernatant and ethylacetate extracts from T. hamatum inhibited mycelial growth of S. sclerotiorum, with EC50 v...
Dilara A. Bukhari1,*, Naureen Fatima1 and Abdul Rehman2

 

...rstuki. The toxicity bioassays with B.t. spores proved that seven B.t. isolates harboring cry11 gene (viz., NF B.t.,1,2,3,4,5,6,7) were toxic to 3rd instar larvae of mosquito, Aedes aegypti. Among seven B.t. isolates, three isolates NF5 B.t. 7.2, NF1 B.t. 1.1 and NF2 B.t. 4.2 were found most toxic which were isolated from moist soil containing decaying cattle waste, dry waste ...

Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil

Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
...r larvae. Two laboratory bioassays, leaf dip bioassay (LDB) and topical bioassay (TB) were developed to check the efficacy of tested chemicals and essential oils. As seen from results, abamectin showed significant mortality (63.5%) of CLM larvae when topical bioassay was performed. Similarly, the percent mortality of CLM larvae was 53.8% after applicatio...

 Asad Sultan1, Rabia Ali1, Rifat Ullah Khan2,*, Sarzamin Khan1, Naila Chand1 and Ambrina Tariq3

...allotted to four dietary bioassay treatments and fed mash red and white sorghum grain with or without phytase enzyme (500 FTU/kg) for 12 days (including 4 days adaptation period). Standard lab protocols were adapted to measure proximate analysis, minerals and phytate content in grain and fecal samples. Sorghum cultivars were different in nutrient profile with red higher in protein content (11.41%). It was observed that phytase inclusion in grain increased the ...
Bushra Siddique1,*, Muhammad Tariq1, Muhammad Naeem1 and Muhammad Ali2
...days before Olfactometer bioassays. For D. rapae, mummified aphidswere collected from wheat crop. Naive females were subjected to olfactometer tests. Seven different combined treatments of semiochemicals and plant extract were applied on filter paper strips at 3% concentration. The filter paper strips were placed in arms of olfactometer. The control arms were treated with n-hexane. Data pertaining to preference of C. septempunctata and D. rapa...

Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Luqman and Muhammad Zeeshan Majeed 

... using standard twig-dip bioassay method according to Completely Randomized Design. Results showed that both factors i.e. insecticidal treatments (F 9, 245 = 146.90, P < 0.001) and time (F 4, 245 = 445.75, P < 0.01) and their interaction (F 36, 245 = 9.20, P < 0.001) had a significant effect on the mortality of mealybug nymphs. The most effective novel chemistry insecticides against D. mangiferae mealybugs were sulfoxaflor, spirotetramat, thiamethoxam...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...od was used for toxicity bioassays according to Completely Randomized Design. Mortality of mealybug individuals varied with plant materials and increased along with the extract concentration and exposure time. Botanical extracts of Azadirachta indica (neem) and Gardenia jasminoides (gardenia) were the most effective with minimum LC50 (20.00 and 42.19%, respectively) and LT50 (47.97 and 71.26 h, respectively) values followed ...
Abdul Hanan1, Talha Nazir1, Abdul Basit1, Shahbaz Ahmad2 and Dewen Qiu1,*
... and V-5) with different bioassay materials (conidia, filtrate and the binary combination of conidia + filtrate) were evaluated against green peach aphid (Myzus persicae) under control condition. Conidial bioassay of fungal isolates were evaluated with three different concentrations (1×106, 1×107 and 1×108 conidia/ml), filtrate bioassay...
Aqsa Pervaiz1*, Rukhshanda Afridi1, Zahra Pervaiz1, Rabia Masood1 and Hira Pervaiz2
...died in a static renewal bioassay for 48 hours and 96 hours (96 hours L.C-50 0.22 mg/L). The study was conducted on 40 fish that weredivided into 4 groups A, B, C and D. Group A was considered as control group.Group B, C and D were exposed to sublethal concentrations of methylmercury 0.044 mg/L, 0.055 mg/ L and 0.073 mg/L in tap water respectively. The fish exhibited tail and fin disruption, bulging of eyes, scales erosion, mucous secretion, hemorrhage, color ...
Riaz Shah1* and Margaret Appleby2
...er before spray (contact bioassay) or after the spray (residual bioassay). In contact bioassay, all of the tested pesticides killed more than 95% of the TSSM after 72 hours except diatomaceous earth (DE). Diatomaceous earth killed only 24.6+3.2% TSSM that was significantly lower than all the other pesticides used. When mites were released onto treated leaf discs (spray residues), only garl...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

Pakistan Journal of Agricultural Research

September

Vol. 32, Iss. 3, Pages 416-561

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe