Search for Baculovirus

Interference of Maternal Derived Immunity Against Vaccination with Baculovirus H5 and ND Inactivated Vaccine

Mahmoud Said^1*, Abd El Satar Arafa¹, Mohammed A. Rohaim^2* and Hussein A. Hussein²

...40) were immunized using baculovirus (H5 and ND) inactivated vaccine via subcutaneous route. Birds (n=20) were then either challenged with 106 EID50 using Egyptian avian influenza H5N1 and Newcastle disease genotype VII. Comparative blood profiling in vaccinated and challenged, non-vaccinated and non-challenged and non-vaccinated and challenged indicated that MDA for H5N1 gradually decreased from 5.1 to 0.4 log2 and disappeared with in a month. While MDA for N...

The Efficacy and Persistence of Spodoptera littoralis Nucleopolyhedrovirus (SpliMNPV) Applied in UV Protectants against the Beet Armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) under Saudi Field Conditions

Sukirno Sukirno^1,2, Muhammad Tufail^1,3,*, Khawaja Ghulam Rasool¹, Said El Salamouny^{1, 4}, Koko Dwi Sutanto¹ and Abdulrahman Saad Aldawood¹

Infection with Spodoptera litura NPV Reduces Food Consumption and Weight Gain of Spodoptera litura Larvae

Ghulam Ali^1,3, Wopke van der Werf² and Just M. Vlak^1,

...v>...

Genomic Homology and Mixed Interactions of Baculoviruses Infecting the Egyptian Cotton Leaf Worm Spodoptera Littoralis In Vivo and In Vitro

Omaima Khamiss

... However, some sequenced baculovirus genomes from closely related viruses are structurally very similar and share overall nucleotide sequence identities in excess of 95%. This work focuses on the comparative essential basis studies to better know complex interactions between baculoviruses infecting the pest in question (Spodoptera littoralis) in relation to stability or mechanism of infection in the same host multiple...

Engineered Norovirus-like Particle as a Vaccine Candidate

Shawki, Khaled K.; Carter, M.J.; Alnashar, Nariman M., El-Farrashl, Mohamed A. and Taher, Sahar

... was expressed using the baculovirus system and the obtained purified protein was examined by EM which showed that the new engineering approach is permitting some form of structural assembly. Our study points a way to produce a more pan-reactive vaccine in future studies by producing antisera directed predominantly at the most conserved S-domain of Norovirus capsid.

...

Impact of Polyethylene Glycol on Newly Established Ovarian Cell Line (Sl-Omi) from Leaf Worm of Spodoptera Littoral's and on Viral Infection

Khamiss l , Omaima; El Helaly , Alexandra and Abol Ela 3, Said

...An infection with Sl NPV baculovirus (Nucleopolyhedrovirus) was carried out on (SL Omi) new established cells in the presence of PEG treatments which demonstrate that PEG has affected the penneability of cells to the NPV infection and enhances the viral replication.

...

Synthesis of the Bovine Rotavirus Major Neutralization Antigen (VP7) Using A Baculovirus Expression System

El-Sabagh, I.M.; Hussein, H.A.; Amer, H.M.; El-Sanousi, A.A.; Reda,I.M. and M.A. Shalaby

...NCDV was inserted into a baculovirus transfer vector under the control of the polyhedrin promotor. A recombinant baculovirus carrying the VP7 gene was constucted through homologous recombination between the baculovirus transfer vector carrying the VP7 gene and Autographa californica Nuclear Polyhedrosis Virus (AcNPV). Infection of Spodoptera frugiperda (SD) cells with

Baculovirus Expression of the Bovine Coronavirus Nucleocapsid Protein in Spodopetra Frugiperda Insect Cells

Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.

...ein was carried out in a baculovirus expression system. The specific RT-PCR product of N gene was cut and extracted from gel using DNA gel extraction kit (Millipore). Eluted DNA was successfully cloned in pBlueBac4.5N5-His TOPO TA baculovirus transfer vector and transformed in chemically competent E. coli. A modified colony PCR assay was utilized to identify the positive bacterial colonies that harbor the recombinant plasmid...