Search for Apoptosis

Increased Oxidative Stress and Apoptosis in Splenic Tissue of Lupus-Prone (NZB/NZW) F1 Mice Infected with Live but not Gamma Irradiated Plasmodium chabaudi

Mostafa A. Abdel-Maksoud^1,2, Fathy A. Abdel-Ghaffar², Azza El-Amir², Gamal Badr³ and Saleh Al-Quraishy¹*

Comparative Study on Induction of Apoptosis in A549 Human Lung Adenocarcinoma Cells by C2 Ceramide or Ceranib-2

Gökhan Kuş^1,*, Hatice Mehtap Kutlu², Djanan Vejselova² and Emre Comlekci³

...ts by trypan blue assay, apoptosis inducing ability by annexin V and JC-1 staining and ultrastructural alterations by transmission electron microscopy. Our data clearly shows that ceranib-2 is more effective than C2 ceramide in promoting apoptosis. The cytotoxic effects of these components cause ultrastructural changes in A549 cells.

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Licofelone Inhibits Proliferation of Rat Hepatoma Cells

Pinar Oztopcu-Vatan¹, Gokhan Kus², Emine Inan³, Melek Gunindi Korkut³, Selda Kabadere^4,* and Ruhi Uyar⁴

...urvival rate and induces apoptosis in a hepatoma cell line (H4IIE). We determined in vitro survival rate with MTT assay, the morphological changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey’s multiple comparison test. IC₅₀values of licofelone were determined as 171 µM for 24 h and 140 µM for ...

Construction and Characteristic Analysis of Omp10 Deletion Mutant of Brucella abortus

Tiansen Li¹, Meiling Huang², Zhen Wang¹,Fei Guo³,Hui Zhang^1,* and Chuangfu Chen^1,*

...ome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results showed the omp10 mutant was dramatically attenuated for survival in macrophages and mice than the parent strain 2308. With decrease in the spleen/body weight ratios of mice infected with omp10 mutants were noted, inhibition of phagosome-Lysosome fusion was dramaticly impaired, and profound increase of

The Predicted Target Genes of miR-122/449a Validation and Effects on Sertoli Cells Proliferation

Lihong Qin¹, Guoliang Zhang¹, Chaojie Zhu¹, Jian Wu¹, Zhihui Zhao^2,* and Yumin Zhao^1,*

...ration, differentiation, apoptosis, and involve in the development of sperm. Meanwhile, DUSP4, PDK4 and FKBP1B were also found to participate in testis development and spermatogenesis. In this study, online software and luciferase reporter gene system were used to predict and verify the target relationship between miR-122 and DUSP4/PDK4, miR-449a and FKBP1B. Thereafter, comparison analysis was done on miR-122, miR-449a...

Effects of Follicular Fluid on Developmental Competence and Gene Expression of in vitro Fertilized Sheep Embryos

Mousa O. Germoush^1,*, Mohsen G. Al-Mutary², Ahmad R. Al-himaidi³, Muath G. Al-Ghadi³, Daisaku Iwamoto^3,4, Yousef Al-anazi³, Aiman Ammari³, Javed Ahmad^3,5, Abdulaziz Al-Khedhairy^3,5

...A transcripts related to apoptosis (Bax and Bcl-2), embryo development (LAMA1, IL-6 and FGF4) and stress (HSPB1) were examined. The results showed that 10% and 20% SFF supplementation exerted no effect on maturation and cleavage percentage whereas 40% SFF supplementation significantly decreased maturation and cleavage. SFF supplemented at 10% concentration showed a significant increase in blastocyst development when compared with other groups. Gene expression ...

Atresia and Apoptosis in Pre- and Postovulatory Follicles of Sharptooth Catfish (Clarias gariepinus, Burchell, 1822)

Şehriban Çek Yalnız ^1,* and Erdal Yilmaz²

...n mammals is mediated by apoptosis, which is a natural occurring cause of cellular death. However, its function in fish is largely unknown. In order to discuss the possible role of apoptosis in fish ovarian follicular atresia and postovulatory regression, the pre and postovulatory follicles (POF) of the freshwater teleost, Clarias gariepinus were observed by light microscopy. The germinal vesicle and the cytoplasmic o...

Possible Antiproliferative Effect of Tolfenamic Acid on Human LNCaP Prostate Cancer Cell Lines

Rıfat Ertekin¹*, Mete Ozkurt¹, Gokhan Kus² and Selda Kabadere¹

...ory medicine stimulating apoptosis in cancer cells like pancreas, oesophagus and lungs. This study aims to determine the role of TA in cell viability and apoptosis in glioma cells (T-98G) and androgen-sensitive human prostate cancer cell line (LNCaP). In our study, 1, 5, 10, 25, 50, and 100 μM doses of TA were applied to T-98G and LNCaP cell lines for periods of 24 and 48 h. Subsequent cell vitality was tested with MTT At...

Protective Effects of Xingnao Enema on Blood-brain Barrier Disruption in a Rat Model of Intracerebral Hemorrhage

Kun Wang^1,2, Yinglin Cui², Xu Zhao² and Changjiang Hu¹*

...he detection of neuronal apoptosis was measured by TUNEL assay. Using immunofluorescence, the expression of claudin-5, ZO-1 and VE-cadherin was detected. The expression of DNA methyltransferase 3b (DNMT3b) and Matrix metalloproteinase-9 (MMP-9) was evaluated by western blot. And microRNA-29b was identified using quantitative real-time PCR. 3). Compared with the Model group, the group treated with XN had a significantly reduced number of dead neurons in hippoca...

Piceatannol Inhibits Akt Activation, Induces G2/M Phase Arrest and Mitochondrial Apoptosis and Augments Cisplatin Efficacy in U2OS Osteosarcoma Cells

Zijuan Li^1,2, Rong Ma², Muhammad Khan³*, Chenchen Liu², Xiaolin Cui² and Yongming Li^1,2*

...ry was used to determine apoptosis and cell cycle profile. Western blot was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase arrest and apoptosis. Further studies showed that piceatannol induces mitochondrial apoptosis as shown by Bcl-2 family proteins modulation. Finally, piceatannol significantly enhanced a...

Piceatannol Inhibits Akt Activation, Induces G2/M Phase Arrest and Mitochondrial Apoptosis and Augments Cisplatin Efficacy in U2OS Osteosarcoma Cells