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Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1

 

Life Sciences International Journal Issue 7 Volume 1
...equencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these strains have shown homology with previously characterized strains from...

Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad

 

British Journal of Virology
...equencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these strains have shown homology with previously characterized strains from...

Timothy Helton

In a Mirror Dimly: Anthropology and Restoring a Sense of Presence to an Empty World
...s an object fit only for analysis discover in it a model of an objectified universe; and those that assume a universe subject only to natural law find in the religions that they study worlds devoid of others–sterile worlds populated only by selves incapable of meaningful relationships. The essay urges that those who see in religions only objects for analysis participate in the construction of worlds inhabited by zombie...

Souvik Ghosh, Nobumichi Kobayashi

British Journal of Virology
...diversity. Whole genomic analysis of RVAs has revolutionized the study of RVA genomics, providing a plethora of conclusive and vital data on the overall genetic diversity, true origin and complex evolutionary patterns of human RVA strains. In this review, we have summarized and discussed the significance of recent research outcomes obtained from whole genomic analysis of common and unusual human RVAs. 

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Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1

British Journal of Virology
...equencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these strains have shown homology with previously characterized strains from...

Yanina Panzera1*, Nicolas Sarute1, Lucia Carrau1, Jaime Aldaz2, Ruben Perez1

..., by means of a detailed analysis of the genetic diversity, evolution, and epidemiology of the virus in several countries. Our study allowed us to establish the presence of four genetic lineages in South America, being the continent with more lineages described to date, underscoring the high genetic diversity of the circulating strains.

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Shahid Ali, Munir Khan

...-10. Stochastic frontier analysis was employed for the estimation of technical efficiency levels and its determinants. Maximum likelihood estimation (MLE) technique was applied to estimate stochastic frontier Cobb-Douglas production function to ascertain the level of technical efficiency. The econometric computer software STATA was applied for the estimation. The estimated value of technical efficiency ranges from 34 to 88 per cent for the farms in the sample,...

Farhad Zulfiqar, Muhammad Ashfaq

...hawalpur. The results of analysis showed that the effect of climatic variables was found significantly higher than that of non-climatic variables. The largest impact on wheat yield was of mean maximum average temperature at the time of maturity, ceteris paribus with one oC increase in temperature the average wheat yield increased by 56.64 kg per hectare in the study area. It was deduced from the economic variable that the level of input used was les...

Mohammed Ali Hussain, Zakiya Ahmed Hassan

... three replications. The analysis of variance showed highly significant differences among maize hybrids for all the traits. The hybrid un44052 x DK took minimum days to tasseling (65.83 days) and silking (68.50 days). Hybrid zp-707 x un 44052 was short statured (155.59 cm) with desirable ear height of 62.93 cm. However, maximum leaf area produced by hybrid DK x Hs in both locations with averages 768.56, 670.26 and 719.42 cm2, respectively, while hybrid sangari...

Gerald Misinzo1,*, Tebogo Kgotlele1, Epaphras A. Muse1, Jan Van Doorsselaere2, Mikael Berg3, and Muhammad Munir4

...amels. Based on sequence analysis of the nucleoprotein (N) gene, the PPRV has been classified into four lineages. Serological investigations in Tanzania indicate that peste des petits ruminants (PPR) was introduced in 2004 in Ngorongoro district bordering Ken- ya before official confirmation of the disease in most districts of Northern Tanzania in 2008. In 2011, the presence of PPRV in goats of southern Tanzania district of Tandahimba bordering Mozambique was ...

Rashid Saleem1*, Zammurad Iqbal Ahmad2, Muhammad Abbas Anees2, Abdul Razzaq3 and Ashiq Saleem1

 

...According to statistical analysis, data revealed that plots treated with PK + inoculation with Rhizobium gave 33% higher mungbean seed yield over control. Similarly, higher values for yield contributing factors were also recorded in PK+ inoculation with Rhizobium. Partial land equivalent ratio (LER) of intercropped maize was 0.96 than respective sole crop. Maximum partial LER of mungbean (0.70) was recorded in intercropping systems. Total LER in maize + mungbe...

Galina N. Leonova1*, Ilya G. Kondratov2, Sergei I. Belikov2

 

... tick bite. Phylogenetic analysis showed that these strains are related to LI/A and LI/K strains isolated in England and Scotland.

 

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Ahmed Sultan Jatoi1*, Shahid Mehmood2, Jibran Hussain2, Hafiz Muhammad Ishaq2, Yassar Abbas3, Muhammad Akram2

 

...however, the statistical analysis revealed significant effect from week 2 to 5, respectively. Times of gain were found significant difference at week 1 to 4; however, at week 5 and 6, it was non-significant. Feed intake (g/bird/week) differed significantly during the entire study period, whereas, feed conversion ratio (FCR) at week-4 only. Imported quail strain (M) performed better in almost all the studied parameters than that of other strains of quails.

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Nisar Ahmad1*, Inayatullah Jan2, Saif Ullah1 and Sidra Pervez1

 

Aqeel Ahmad, Zammurad Iqbal Ahmed, Irfan Aziz*

 

...next to it. The residual analysis confirmed the findings. Similarly sorghum gave higher returns than mung bean when grown after lentil. The studies showed that partial budget analysis was a reliable technique for farmers to optimize fertilizer recommendations and harvest maximum returns from their investment in agricultural business.

 

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Kemi Funmilayo Omotesho1*, Isreal Ogunlade1, Opeyemi Eyitayo Ayinde2

...scale and the regression analysis. The study revealed that farmers perceived the level of accountability of extension to farmers in the study area as poor. Level of education, access to training and membership of farmer-groups negatively influenced accountability at p<0.05. Based on the findings of the study, it is recommended that agricultural extension policies that will ensure accountability of extension to farmers should be put in place. Farmers should ...

Hina Fatima1*, Muhammad Azeem Khan2

...ouglas Translog frontier analysis has been done. A primary research is conducted in the area of Rahim Yar Khan. Adoption of Modern wheat varieties is important for more profit as well as for enhancing the wheat productivity. However, modern wheat varieties have not been adopted widely in Pakistan. Farmers make use of traditional wheat varieties, whose yields are quite below the modern wheat varieties. The result of the study reveals that the parameter estimate...

Imdad Sohu1, Allah Wadhayo Gandahi2*, Ghulam Raza Bhutto2, Muhammad Salim Sarki2, Rabail Gandahi3

...bservations and chemical analysis of the soil and plant samples were recorded. The application of organic sources of farmyard manure and poultry manure in combination with inorganic NPK fertilizers had shown positive effect on chickpea plant height, number of branches plant-1, number of pods plant-1 and seed index. The growth and yield of chickpea significantly increased with the collective application of chemical fertilizers and organic manures. It is conclu...

Shahid Iqbal Awan1*, Syed Dilnawaz Ahmad2, Muhammad Amjad Ali3, Muhammad Shahzad Ahmed1, Aurangzeb Rao1

...: justify;">Multivariate analysis was used to assess the diversity in 176 wheat genotypes for morpho-physiological traits. Simple correlation coefficients indicated that grain weight had a significant positive relationship with residual transpiration, osmotic adjustment, cell membrane stability, flag leaf weight, specific flag leaf weight, while negatively correlated with specific flag leaf area. These results indicated the importance of physiological traits a...

Ryan T. Cragun1*, Joseph H. Hammer2, Michael Nielsen3

... one confirmatory factor analysis of the NRNSS supported the hypothesized two-factor solution: (a) institutional religiousness and (b) individualistic spirituality. The NRNSS also demonstrated convergent validity through theoretically-expected correlations with established measures of religiousness and spirituality (the Cross-Cultural Dimensions of Religiosity, Humanistic Morality, and Traditional Religious Morality scales). In summary, the NRNSS may work as a...

Rabnawaz1*, Muhammad Jamal Khan2, Tahir Sarwar2, Mohammad Jamal Khan3

...allowance). Quantitative analysis of the yield per unit volume of water applied was carried out using dummy-regression model. The difference in means for maize, sugarcane and tomato crop was found highly significant while in case of wheat it was non-significant. This study concludes that the public system remained more productive with high CWP at farm level for all the major crops. However, there is a great potential for increasing CWP ...

Ali Muhammad*, Muhammad Ayub

...e; 0.05. Physicochemical analysis showed that decrease was observed in ascorbic acid (15.18 to 7.21), pH (4.56 to 3.9) and TSS (5 to 2.5). Standard deviation showed the least variation in data. Results show that on nutritional basis RW1 was found best followed by RW2.

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Muhammad Nawaz Kandhro*, Habib-Ur-Rehman Memon, Muhammad Ali Ansari, Ahmed Naqi Shah

...de. The statistical analysis of data showed that interculturing, Dual Gold, and sorghum and sunflower water extracts caused significant reduction of weeds and increased seedcotton yield as compared to weedy check. The combined application of sorghum @ 15 L ha-1+ Dual Gold @ 1.25 L ha-1 resulted in weeds mortality upto 66.6%, produced seedcotton yield of 3961.4 kg ha-1 and gave net benefit of Rs. 2,20,029. Sunflower applied @ 15 L ha-1 ...

Brittany D. Rife 1,2, Marco Salemi 1,2*

E-mail | salemi@pathology.ufl.edu

...Bayesian phylogeographic analysis has recently been at the forefront of understanding epidemiological processes through the reconstruction of evolutionary history and spatial dissemination of pathogens. Infection of a single host can be viewed similarly as an epidemic spread on a microscale, creating a unique opportunity for the application of phylogeography at the intra-host level, which has until recently been limited by dataset quality a...

Rick Repetti

...ustify;">I argue that an analysis of Buddhist meditation theory and practice may be used to ground a model of the possibility of free agency that stands up against four powerful arguments for free will skepticism in contemporary analytic philosophy: Peter van Inwagen’s consequence argument, which asserts that if choices are lawfully necessary consequences of prior events, then they are unfree; Derk Pereboom’s two arguments for h...

Oladipo Elijah Kolawole*, Oloke Julius Kola, Awoyelu Elukunbi Hilda, Olasunmibo Oluwatobi Gabriel, Ogunyale Tolulope Temitope

E-mail | koladipo2k3@ yahoo.co.uk

...7.89%). The multivariate analysis between age and CD4 count shows highest prevalence in the age range 31-40 years with CD4+ count less than 200cells/μl. The seroprevalence shows there is a significant difference between age and CD4+ cell count. The result provides the evidence that Hepatitis E virus is present among HIV individuals in Ogbomoso and they are more susceptible to the virus.

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Temitope Oluwasegun Cephas Faleye1, Johnson Adekunle Adeniji1, 2 *

...ubjected to phylogenetic analysis. Both sites yielded two isolates each on MCF-7 cell line while on RD cell line one site yielded one and the other seven isolates. None of the twelve isolates replicated on L20B cell line. All were positive for both the panenterovirus 5l-UTR and partial VP1 PCR reactions while only the four isolates recovered on MCF-7 where positive for the EV-C-3Dpol/3’-UTR-PCR assay. The eleven isolates with exploitable VP1 sequence dat...

Youssef Lhor1*, Mounir Khayli1, Mohamed Bouslikhane2, Mehdi El Harrak3, Ouafaa Fassi Fihri2

Email: y.lhor@yahoo.fr

...easures and disease risk analysis, this entomological survey was performed on the risk of transmission of bluetongue virus (BTV) and identification of the main breeding sites of Culicoides. From April 2009 to March 2010, the study was carried out at 14 sites distributed across the country. Results indicated that the two most abundant Culicoides species were Culicoides imicola (94.2-95.85%) and Culicoides newsteidi (2.21-2.72%). The rate of ...

Monireh Ahmadi Bani1*, Mostafa Kamali2, Iman Borji3, Soheyla Aslani3, Francis Fatoye4

...nal method for stability analysis showed no difference between two conditions in one minute standing (p ≥ 0.05). However, the new method confirmed that stability parameters significantly decreased in five minutes standing (p<0.001). Moreover, patients were unstable in standing during the fourth and fifth minute. Taken together, diagnosis based on five minute standing using the Romberg test appear to be sensitive and accurate than one minute.

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Ali Mohammadi, Mohammad Hossein Ebrahimi

... was evaluated by motion analysis system. It has been shown that severity of pain and overall function of knee joint were improved following the use of this implant. Although it has been mentioned that KineSpring knee implant is an effective method to relieve pain and improve function in Knee OA, there are incomplete evidences to support this claim. Therefore, it is recommended to perform high-resolution clinical trials to evaluate the...

Fatemeh Kheiri

...ted by use of the motion analysis system and a Kistler force plate. The stability of subjects during standing and their energy consumption while walking were analyzed by the use of a Kistler force plate and a polar heart rate monitoring. The difference between the kinetic and kinematic between sound and prosthetic sides was evaluated by two sample t-test. The physiological cost index of amputees varied between 0.42 and 0.74 beats per minute. there was no signi...

Seyed Mohsen Mirbod

...ted by the use of motion analysis system and a force plate (Kistler). The mediolateral excursion of the knee joint decreased while walking with the knee orthosis in contrast to normal walking. Moreover, the mediolateral moment applied on the knee joint decreased significantly. This new design of the orthosis improves the performance of the subjects during walking. It carried same performance as that of the knee cage, and additionally is more cosmesis, eas...

Rehmat Ullah1*, Muhammad Zafarullah Khan1 and Kalim Ullah2

...fy;">Constraints and gap analysis of Model Farm Services Center (MFSC) approach was investigated in District Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan, during year 2015. For the present study, a sample of 306 respondents was selected based on Sekaran sampling technique while using descriptive research design. Well-prepared and pre-tested interview schedule was utilized for collection of data. Descriptive statistics i.e. frequency, percentages and rank ord...

Moorthy S. Muthuswamy

...ial guide to life. A new analysis of the 2013 Pew data shows us that religious leaders’ political and religious influences correlate with sharia’s popularity, and in turn, can correlate with politically motivated violence. In particular, the top three terrorist groups in 2013 responsible for the most killings have had religious leaders in very senior positions. Calling the sharia bluff as a core part of a counterterrorism approach may be central to...

Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4

...1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb. Our findings report the emergence of PPMV-1 in Egyptian pigeons and the potential role these wild birds can play in the transmission and evolution dynamics of PPMV-1.

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Qaizar Ahmed1, Fida Mohammad2, Hidayat-ur-Rahman2, Sheraz Ahmed2* and Fakharuddin2

... were analysed in pooled analysis and individual environments. Heterosis over better parent was determined for all traits across environments. Heterobeltiosis estimates reflected both positive and negative values, suggesting additive as well as non-additive gene actions in the expression of traits. Performance of hybrids was environment-specific. However, heterobeltiosis estimates ranged between -7.9 and 25.90 for leaf area, -6.76 and 130.8 for number of cured...

Gurrappa Naidu Govindaraj, Vinayagamurthy Balamurugan*, Habibur Rahman

...ly. Further, sensitivity analysis under Ceteris paribus, revealed a loss of INR 8058.8 million and INR 24174.1 million at the minimum (5%) and maximum (15%) incidence levels, respectively. Thus, the estimated loss due to PPR in sheep and goats is dependable till the large-scale primary survey estimates are available in India.

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Keyvan Sharifmoradi1*, Nader Farahpour2

...d in the study. A motion analysis system (Vicon Motion Analysis System) and two Kistler force plates were used to measure forces applied on the legs. Data were evaluated using independent t-test with significant point set at p< 0.05. There was no difference in cadence between left and right of normal subjects and those with PD. However, there was a significant decrease in walking speed and stride length in both sid...

Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*

 

...uenced. The phylogenetic analysis revealed that PPRVs circulating in Bangladesh belonged to Lineage IV and they formed a separate sub-cluster along with recent isolates from Nepal, Bhutan and China. On genetic analyses, it appeared that the N gene of PPRV is less conserved as compared to the F gene. Two unique amino acid substitutions (K423Q and E426G) were observed in Bangladeshi and Chinese isolates in the partial sequence of N protein.The recent Bangladeshi...

Muhammad Jurial Baloch1*, Ghulam Murtaza Channa2, Wajid Ali Jatoi2, Abdul Wahid Baloch1, Imdad Hussain Rind1, Muhammad Ahmed Arain1 and Ayaz Ali Keerio1

...dojam, Pakistan. Genetic analysis of yield traits from half diallel crosses was carried-out. Mean squares due to GCA and SCA were significant for days to 75% maturity, tillers plant-1, spike length, spike density, grains spike-1, grain yield plant-1, seed index and harvest index. Significance of GCA and SCA variances suggested that both additive and non-additive genes were controlling these characters. The magnitude of SCA vari...

 Farmanullah Khan, Samad Khan, Wiqar Ahmad and Imran Khan

... concentration. Economic analysis showed that NPK75FYM20 rewarded the maximum net income of Rs. 43108 ha-1, indicating its superiority for profitable wheat yield under rain-fed conditions. It can be concluded from these results that the application of plant nutrients from a variety of natural and manufactured fertilizer sources improve crop yield as well as augment the fertility of soils suffering from water erosion hazards.

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Hina Fatima, Muhammad Azeem Khan, Mahreen Zaid-Ullah, Abdul-Jabbar, Khurram Nawaz Saddozai

...ncy and the productivity analysis of BT- Cotton varieties with those of the conventional non-BT-Cotton varieties. The population  of  this research covers the area of Rahim Yar Khan of Pakistan. Two hundred and fifteen farms were selected from each of the NonBT-cotton farms and BT-cotton farms. The Cobb- Douglas Stochastic Frontier Analysis (SFA) has been employed to determine the technical efficiency of these grow...

Muhammad Mushtaq, Umer Sadique, Naila Chand, Iftikhar Ahmed, Said Sajjad Ali Shah, Ijaz Ahmad, Imran Ullah and Muqadar Shah

...as statistical model for analysis. Lipid profile and serum glucose was significantly improved. Lipid profile and serum glucose was significantly reduced in all treated groups. Mean cholesterol was (157.66 mg/dl), triglyceride (65.64 mg/dl), HDL (58.88 mg/dl) while no alteration in LDL contents. Significantly effect of water based infusion was notice on mean serum glucose (73.83 mg/dl) Observation of the study indicating that water based infusion of W. somnifer...

Shagufta Jabeen1, Muhammad Saqlain Raja1, Sadia Saeed1, Muhammad Mobeen Zafar1, Rizwana Abdul Ghani1, Abid Mahmood1, Muhammad Fiaz2, Pakeeza Arzoo Shiaq1, Shahid Mahmood Baig3, S.M. Saqlan Naqvi1 and Ghazala Kaukab Raja1

...stics were used for data analysis. Early age of addiction onset, low literacy rate, peer pressure, occupation type, and stress are among risk factors increasing susceptibility towards heroin addiction in our studied cohort. Drug abuse initiated at an early age seems to trigger transition to heroin addiction under the influence of environmental risk factors.

..., functional and pathway analysis of the mammary gland genes were performed to find mastitis resistant genes, which helped us to identify prime genes of mammary gland that are more resistant to mastitis, and analysis of those resistant genes helped to find the solution of mastitis and immunity related diseases.

...c Gobiinae. Phylogenetic analysis also demonstrated clade Amblyopinae included all three species and that O. lacepedii has been proved to be a much closer affinity to O

Metin Duru1*, Asuman Arslan Duru1, Köksal Karadaş2, Ecevit Eyduran3, Harun Cinli4 and Mohammad Masood Tariq5

...t and week through CHAID analysis. A total number of eighty, 56 weeks old Hy-Line White laying hens (commercial type) of nearly similar initial body weight (1360.6±14.25 g) were assigned into 5 experimental groups (control group and 4 treatment groups) each including 16 and maintained individually in cages of 35 x 40 x 45 cm. The strongest correlations were found for egg weight-egg width (r=0.910, P<0.01), haugh unit-albumen index (r=0.876, P<0.01...

Robina Karim and Dawood Jan

...stic market. This stated analysis thus supports partial acceptance of hypothesis, which states that “Pakistan’s foreign trade is expected to be largely better off once WTO’s regime is fully implemented in Pakistan and rest of the world”. When econometric analysis was carried out for pre and post WTO periods, it has been concluded that the external trade of Pakistan has been increased but not up to the...

Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand

... for antibiotics residue analysis based on electrochemical signal generation. Aptamers possesses the strong potential as receptors for the development of biosensors for antibiotics detection; therefore, a specially designed aptamer specific to an antibiotic may be suitable for this purpose. In this review, the importance of detection of antibiotic residue contamination, reported analytical methods, advancement in biosensing platform especially regarding electr...

Ambreena Hafiz1, Tanzeela Riaz2 and Farah Rauf Shakoori1*

...nce thirteen years. From analysis of metabolic profile, it was found that soluble proteins, glucose contents and free amino acids increased, whereas glycogen and lipid contents were reduced in all deltamethrin-resistant populations as compared to deltamethrin-susceptible population. Soluble Proteins were significantly elevated (79, 100 and 37%) in 4th and 6th instar larvae and adult beetles of Gujranwala, (14, 24 and 14%) in Okara and (14, 13 and 2%) in D.G Kh...

Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2 

...ype and allele frequency analysis showed that SNP G62976A and SNP G50831C of the HTT gene in chicken had some effects on reproduction traits, and the genotype GG was the advantageous genotype. Additionally, four HTT haplotypes (H1: GG; H2: GC; H3: AG; H4: AC) and their frequency distributions were estimated using the phase program. Haplotypes combinations constructed on these two SNPs of HTT gene were associated with some reproduction traits. In particular, di...

Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2

...lts of the phytochemical analysis showed that the extract has high polyphenolic content while has relative low flavonoids contents. In the antiradical scavenging property test the extract showed at 64.1 μg/ml 50% DPPH radical scavenging activity and in the MTT assay the extract has relatively low cytotoxicity where the IC50 value of extract against Vero cells was 1078.69 µg/ml. Furthermore, the extract exhibited a considerable anti-HSV-1 and anti-HSV-...

Ali Raza Awan*, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim

...b gene. The Phylogenetic analysis of the Cytb gene indicated that the Pakistani wild Rose ringed Parakeet was mono-phyletically claded with P. k. manillensis with a sequence similarity of 99.37%. Comparative analysis indicated 4 Single Nucleotide Polymorphism (SNPs) sites in the Pakistani wild Rose-ringed parakeet’s Cytb gene which tags the peculiarity to the Pakistani wild Rose-ringed parakeet. This is the first repor...

Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4

...ign: justify;">Microbial analysis of Indian flying fox, Pteropus giganteus ejecta roosting at Jinnah and Lalazar Gardens, Lahore was carried out from January, through December, 2011 and a total of twelve fungal and twelve bacterial genera were isolated. Four fungal (Candida, Fusarium, Penicillium and Saccharomyces) and two bacterial genera (Klebsiella and Nocardia) were isolated from bolus only, three fungal (Cryptococcus, Histoplasma and Trichophoton) and six...

Imed Ben Salem*, Aymen Ben Ibrahim, M’barek Chetoui and Said Nouira

...eals with morphs-genetic analysis of eight species viz. Lemniscomys barbarus, L. bellieri, L. griselda, L. limulus, L. macculus, L. rosalia, L. striatus and L. zebra; which have practically similar external morphologies although they spread in completely different geographical areas. The approach adopted was to identify landmarks on the skulls of all specimens using the tpsDig software, and then analyzing them through the program MorphoJ. Our results show that...

Atta ur Rehman1, Sher Ali2 and Sajid Malik2*

Dein Simon

...f evidentialism. From my analysis of these characteristics I conclude that the movement is fundamentalist

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Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan

...agglutinin gene sequence analysis of the two strains reveal high similarity with difference close to cleavage site (HARSSR/ GLF) and (PARSSR/GLF) for V3 and RSF/1 respectively. Results of growth kinetics in PBMCs showed that V3 replicated more efficiently comparing to RSF/1 at all-time points. Further, strain V3 induced significant up-regulation of IL-1b and IL-6 at 24 hours post infections (hpi) comparing to RSF/1. However, both strains induced significant up...

Muhammad Shafiq and Tahir Maqsood

...tion. Regarding chemical analysis concentration of boron in both wheat straw and grains increased with B application but there was no effect of fertilizer B on NPK concentration of straw and grains. These results are very heartening; signifying that for any soil, B adsorption isotherm need to be worked out for location precise fertilizer B use.

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Oladipo Elijah Kolawole, Oloke Julius Kola and Awoyelu Hilda Elukunbi

...6.36%). The multivariate analysis using age and the CD4 count showed highest prevalence (27.27%) among the age ranging from 41 to 50 years with the CD4+ count of 350- 500cells/µl. The result from this study highlight that EBV IgM is prevalent among individuals infected with HIV in Nigeria and may warrant future investigations to ascertain the impact of co-infection on public health.

...

Sezgi Arman* and Sema Isisag Ucuncu

...S-positive). Statistical analysis were confirmed that, the numbers of acidic and neutral cells of per unite square of epithelial area were significantly different.

...

Sezgi Arman* and Sema Isisag Ucuncu

...S-positive). Statistical analysis were confirmed that, the numbers of acidic and neutral cells of per unite square of epithelial area were significantly different.

...

Nighat Sultana1,2,*, Shazia Iftikhar1, Nafeesa Qudsia Hanif2 and Iffat Tahira2

...) and ochratoxin A (OTA) analysis showed that AFB1was present with high frequency in fresh (37.5%) and ensiled (41.66%) fodder, with an average concentration of 9.49 and 8.36ng/g respectively. Aflatoxin B2 was detected in only two samples (16%) (1.2 and 1.3ng/g), aflatoxin G1 and G2 were not found. Ochratoxin A was found more frequently in fresh (54.16%) than in ensiled (20.86 %) samples with a mean of 8.06 and 4ng/g respectively. Average values for total afl...

Wang Tian1, Huayong Zhang1,*, Jian Zhang2, Lei Zhao1, Mingsheng Miao3 and Hai Huang1

...canonical correspondence analysis (CCA) revealed that water temperature, Secchi disk depth (SD), total phosphorus and phytoplankton biomass were the most significant factors that influenced zooplankton. Strong correlations between SD and Brachionus leydigi, Chrysophyta and Diaphanosoma leuchtenbergianum, Cryptophyta and Theopilium, Ascomorpha ovalis, Hexarthra mira were observed during the CCA. These zooplankton species may be used as indicators of releva...

Abdul Baset1, Qun Liu1,*, Muhammad Tariq Hanif1, 2, Baochao Liao1, Aamir Mahmood Memon1,3 and Muhammad Mohsin1

...A (catch and effort data analysis) consists of Fox, Schaefer and Pella Tomlinson models and ASPIC (a surplus production model incorporating covariates) contain Fox and Logistic models. The average annual landings of S. longiceps stocks were 38 353 t while the highest and lowest 65 050 t in 1994 and 26 937 t in 2009 was recorded correspondingly. The MSY was estimated using the initial proportion (IP) value of 1 because the starting catch was approximately 100% ...

Oluwasogo David Olorunfemi, Oluwasegun Adetokunbo Adekunle, Felix Olayinka Oladipo, Temitope Oluwaseun Oladele and Oladimeji Idowu Oladele

...ther (82.5%). Chi-square analysis revealed a significant relationship between the respondents’ training needs on value addition initiatives and their age, educational level, years of experience and fish farm income. The value addition initiatives use profile of the fish farmers was still very low among the respondents and training was highly needed on value addition initiatives in seven major areas. It was recommended that robust training programmes and ...

Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

...imadzu). The statistical analysis indicated that non-significant association existed between SOD1 (rs2070424) gene polymorphism and RA (p>0.05). Results of HWE estimation indicate that allele frequencies were not deviant from HWE in RA group. The results of present study indicates that Pb and Cr concentration differ significantly between AA, AG and GG genotypes of SOD1 (rs2070424) gene polymorphism in RA patients as compared to control group (p<0.05). Th...

Neelum Andaleeb and Munir Khan

...unction and cost benefit analysis were used to arrive at the main conclusion. Results show that feed cost was the major component of total cost followed by labor cost. Average milk produced by buffalo and cow was 180 and 135 liters per month, respectively. Average total costs of milk production of buffalo and cow were Rs.12835 and Rs.10070 per month respectively whereas the average net income per liter from the buffalo and cow milk was Rs. 29 and Rs. 5 thus be...

Fida Mohammad, O.S. Abdalla, Sheraz Ahmed, Fakharuddin and S. Rajaram

...explained by the cluster analysis of environments. Clustering of five test environments based on Shifted Multiplicative Model (SHMM) exposed the major role of grains spike-1 and harvest index in production of grain yield. Though the rate and distribution of rainfall during the cropping season were the main contributing factors in the varied performance across years, however, the grain yield of synthetic derived wheat lines across different environments was not...

Amjad Ali and Abbas Ullah Jan

...A) was employed for data analysis using computer software STATA. Result illustrates that technical efficiency of sugarcane crop for the period under study in Khyber Pakhtunkhwa ranges from minimum 0.47 to maximum 0.98 with mean value of 0.84 percent. This mean that with same resource base average sugarcane growers can increase his yield up to 14% and most technical inefficient grower can increase his per acre yield up to 52% in order to achieve technical effic...

Muhammad Jamal Nasir, Anwar Saeed Khan, Said Alam and Saeed Akhtar

...al (114 households). The analysis of acquired data reveals that in 2008-09 tube well irrigated land account for 81.67 acre which decreased to 37.46 acre in 2013-14, after the dam construction. The number of parcels irrigated by tube well decreased from 158 to 75 in 2013-14. Today canal irrigation is the largest source of irrigation in the Mouza, which accounts for 56.09 acre. The analysis also reveals that the centuries old ...

Muhammad Shahzad Ahmed and Dilnawaz Ahmed Gardezi

...on, phenotypic diversity analysis and their flowering behavior sugarcane under local conditions. Two year data revealed that genotype HSF-242 showed maximum mean values for, internodes length, brix percentage and reducing sugar while S-08-FSD-19 showed maximum values for number of tillers and stem girth, whereas S-05-FSD-307 revealed maximum values for plant height and number of nodes. Analysis of variance revealed highly si...

Hina Fatima, Lal Almas and Bushra Yasmin

...ered the cost efficiency analysis of cotton-melon cropping system under tunnels. The data were collected from 150 farmers; those were cultivating cotton-melon crops in combination under tunnels. The study area was Punjab, Pakistan. The stochastic cost frontier analysis was used to estimate the allocative efficiency of cotton-melon cropping systems efficiency. The result illustrates that none of the farmers to be optimally us...

Naushad Khan1*, Shahnaz Akhtar1, Munir Khan1, Shaista Naz2, Javeria Tanveer1 and Muhammad Kaleem3

...d t-test and correlation analysis were used to analyze the data. It was found, that ZTBL credit program has overall a positive effect on maize productivity in the study area. Majority of the credit beneficiaries were small scale farmers who obtained medium term credit followed by short term credit type. Thus, higher amount of credit was disbursed under the category of medium term followed by short term. The average yield, cost and return for the maize crop sho...

Ibni Amin Khalil* and Raziuddin

...ted to combining ability analysis. For the inheritance of the studied trait (seed yield plant-1), additive as well as non-additive genetic mechanisms were found important due to significant effects for both components i.e. General Combining Ability (GCA) and Specific Combining Ability (SCA) effects. Maximum GCA was shown by L-7 and T-1; whereas high SCA was noted for L-6 and L-7 with good ability to combine with T-3 and T-1 respectively, and could be utilized ...

Muhammad Afzal1 and Murad Khan2*

...stify;">To make economic analysis of broiler poultry farms, a survey research was carried out in district Lower Dir in 2015. Four tehsils were selected namely Adinzai, Balambat, Timergara and Sumerbagh on the basis of purposive sampling technique. From these tehsils, 92 farms were selected randomly from 460 poultry farms. The sample farms were categorized into three groups i.e. small, medium and large. For analysing the data, descriptive statistics and gross m...
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...morphology and molecular analysis of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Adult P. websteri exhibit a high degree of morphological plasticity in the palp pigmentation pattern, the shape of the anterior edge of the prostomium, the shape of the major spines on chaetiger 5, and the shape of the pygidium. The COI gene sequence demonstrated that the intraspecific distance of P. websteri was 0.33%, whereas the interspecific dist...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...sequencing and molecular analysis of fusion (F) and hemagglutinin (HN) genes of NDV strain isolated from an outbreak in a peacock flock was undertaken. Proteolytic cleavage site of F0 showed a cleavage motif (112RRQKR↓F117), representative of a velogenic serotype. Moreover, based on F and HN gene nucleotide sequence analysis, the isolate clustered as genotype VII closely associated to th...
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
...ignificance with one way analysis of variance followed by Tukey’s multiple comparison test. IC50 values of licofelone were determined as 171 µM for 24 h and 140 µM for 48 h applications. Used as a positive control, 250 µM 5-Fluorouracil decreased the cell survival by only 40 % after 48 h, but licofelone treatment with the same dose and time duration decreased the number of surviving cells by 87 %. Treatments with 150, 200 and...
Mehmet Cilbiz1* and F. Banu Yalim2
...f Relative Yield/Recruit analysis estimated Emax>E, so in this context stock of O. mykiss was not overexploited in Karacaören I Dam Lake.
...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...furthered to biochemical analysis. Pearson’ Chi-square test at nighty five percent confidence interval was used to analyze the collected data. Antibiotic sensitivity was checked with twenty four antibiotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67%, 50/120) form of mastitis on overall milk collected data. Pathogen type was crowned by Staph. aureus with 74.5% (47/63) prevalence, following ...
Jun Cui, Xiaoxu Zhou, Zhicheng Wang, Derong Kong, Xuemei Qiu, Hongdi Wang and Xiuli Wang*
...ical proteins. The SMART analysis revealed that CcWap65 contains three hemopexin-like repeats (E-value < 0.05). The crucian carp Wap65 was mainly expressed in liver, with limited expression observed in intestine, skeletal muscle and kidney. CcWap65 was significantly up-regulated in the liver of crucian carp after heat stress, suggesting that increase in Wap65 gene may be related to high water temperature stress and play important roles in high...
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
...e LvCypA after real time analysis revealed that expression is in order of muscle, gill, lymphoid organ and hepatopancrease. 
...
Fermín Lopez-Uriostegui1, Jesús T. Ponce-Palafox2,*, Fabiola Lango-Reynoso1, María del Refugio Castañeda-Chavez1, Virgilio Eugenio Arenas-Fuentes3 and Fernando Vega-Villasante4
... their stomach repletion analysis. Samples were taken four-hly over a 24 h cycle. In general it was found that stomach repletion index showed both species feed 24 h, but forage activity differs in intensity in the two species. In M. tenellum repletion index was greatest during the day and M. americanum in the night.
...
Andrea Rezic1, Ivica Boskovic2, Piera Lubinu3, Marina Piria1, Tihomir Florijancic2, Massimo Scandura3 and Nikica Sprem1*
...ng discriminant function analysis and parametric test did not reveal significant differences between the sexes, while dorsal mean skull shape was statistically significant (p<0.05). The shape comparison between males and females using permutation tests with procrustes distance yielded with significant results for both skull and mandible shape. Further studies are required with increased sample size and number of landmarks so as to obtain a more accurate exp...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...ough logistic regression analysis, it was observed that, in Tamil population the CC and CT genotype carriers have higher odds of developing myocardial infarction (OR – 1.64 and  2.04 respectively) and for developing hypercholesterolemia (OR – 2.02 and 2.13 respectively). In the total population, the CC genotype carriers have significantly lower HDL-C (0.82 ± 0.04 mmol/L Vs, 0.9 ± 0.05 mmol/L (p<0.05)) and higher LDL-C (3.7 &pl...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2
...cal examinations and PCR analysis. The results showed, 20 chickens as positive for ALV-A (2.73%, 95% CI 1.7-4.2) and these chickens were found to be positive for ALV-A with the further distribution of 2.91% (95% CI 1.0-6.7), 3.70% (95% CI 1.5-7.5), 2.48% (95% CI 0.5-7.1), 3.13% (95% CI 0.6-8.9) and 1.30% (95% CI 0.2-4.6) in Anhui province of Huaibei, Suzhou, Bozhou, Bengbu and Fuyang, respectively. The present survey showed that free-ranging chickens of Anhui ...
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
... study indicate that DEG analysis of RNA-seq data help us better understand the precise mechanisms of PEDV in pig PBMC. 
...

John G. Bruno

...y for more sophisticated analysis that could lead to improved rickettsial serodiagnostics and aptamer-based vaccines.

...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...tan cities. Phylogenetic analysis indicates that M. m. musculus migrated from northern China to eastern China and that M. m. castaneus invaded from southern China. The two subspecies form a hybrid zone in eastern China. There were three major well-differentiated clades within M. m. castaneus, which were designated as CAS1, CAS2 and CAS3 and confirmed previous internal sublineage differentiation of this subspecies. Of the three clades, only...
Jun Gao1,2, Liang-Liang Yue3, Xianhuan Jiang1, Liju Ni4, Muhammad Aqeel Ashraf5, Yuxun Zhou,1 Kai Li1,* and Junhua Xiao1,*
...nd Bayesian phylogenetic analysis, highly supported all the M. fortis individuals clustering into one monophyletic lineage. Three main clades are recovered within M. fortis: (1) North group; (2) South group; and (3) GX group. The North Group distributed on the north side of Qinling Mountains-Huaihe river line as well as the South Group was on the south. It suggests this geographic barrier played an important role in differentiation of M. forti...
Adel Eid Mohamed Mahmoud*
...growth performance, meat analysis and feed intake of growing lambs. Fifty one Barki lambs with body weight of 30±0.57 kg; in three groups (17 animals, each). Experimental rations were composed of concentrate and clover hay in a 60:40 concentrate: roughage ratio.There was no effect of bakery by-products inclusion on nutrients digestibility and nutritive values except digestion coefficients of crude protein and digestible crude protein. Ruminal pH values ...
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...ter maze test. Pathology analysis of treated animals showed obvious gliosis and neurofibrillary tangles in the cerebral hippocampal area. These results suggest that the tree shrew can recapitulate the major features of AD pathogenesis. To provide the basis for further studies with this new animal model, we used high-throughput sequencing to analyze changes in the hippocampal transcriptome induced by injection of the three AD agents. These results open the door...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...ly analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response on the post-thawed spermatozoa motility, viability and membrane integrity of Achai bull.
...
Urooj Fatima1, Arslan Mehboob2, Muhammad Abid3, Muti-ur-rehman Khan4 and Tahir Yaqub4*

 

...ositive in PCR. Sequence analysis of the selected positive samples revealed that both CPV-2 and new CPV-2a strains are circulating in the domestic dog poputlation in Pakistan. This pioneer research on the molecular characterization of CPVs in dogs would establish foundations to carry out effective control strategies in future.
...
Muhammad Qadir Ahmad¹*, Farheen Ashraf Raza¹, Abdul Qayyum¹, Waqas Malik¹, Rao Wali Muhammad2, Muhammad Asif Saleem1, Amir Hamza2, Ahmad Baksh Mahar3
...ed for studied traits by analysis of variance. Pearson correlation analysis depicted significant positive association between NL and BW. SV also revealed positive association with SI. BW showed positive association with NLB, NS and SI. LA showed positive association with NL. Path coefficient analysis was utilized to partition the direct and indirect effects of different traits on BW. Path ...
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...6.0 and 7.0 mm. The HPLC analysis of apricot suggested that 5.0 gm of apricot contain 1.04 µg/ml of chlorogenic acid on dry weight basis. It is concluded that apricot may be an alternative, natural and supplemental treatment for diseases associated with these pathogens.
...
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...ain 26695. Computational analysis of the genome showed 6 transcriptionally active pseudogenes that can produce stable mRNA secondary structure compared to their functional parents. Moreover it was observed that their putative protein products will be thermodynamically stable. The sequence-based predictions suggested that the pseudogenes-derived proteins may involve in different biological functions like translation, energy metabolism, amino acid metabolism and...

M. D. Maji, N.K.Das, S. Chatterjee, A. Ghosh and A.K. Bajpai

Forecasting models of bacterial leaf spot disease of mulberry for Birbhum district of West Bengal
...down multiple regression analysis revealed that the forecasting of BLS could best be done from min temp, minimum relative humidity and number of rainy days.

...

Basudeb Dasgupta, Partha Dutta and Srikanta Das

Biological control of foot rot of betelvine (Piper betle L.) caused by Phytophthora parasitica Dastur
...luorescens over two year analysis of pooled data gave the best c : b ratio.

...

Rashid Pervez, Santhosh J. Eapen, S. Devasahayam and M. Dinsha

Characterization of entomopathogenic nematode, Stein-ernema carpocapsae from ginger (Zingiber officinale Rosc.) rhizosphere in India
...ed by ITS-rDNA sequences analysis. Phylogenetic was constructed for studying relationship with known isolates. Pathogenic potential of the isolate of S. carpocapsae (IISR-EPN 06) on the larva of shoot borer (Conogethes punctiferalis), hairy caterpillar (Euproctis sp.) and greater wax moth (Galleria mellonella) was found under in vitro condition. Further this isolate displayed high virulence on above insect species. This study reported occurrence of a isolate o...

Dhananjoy Mandal 2K. Baral 3M. K. Dasgupta

Developing site-specific appropriate precision agriculture
...ced thematic maps, image analysis, geostatistics for spatial analysis, land, soil and physiography, farmers’ socio-economic status, meteorological data, crop suitability, crop and pest management, processing strategy and transport, market arrival, demand and price are provided, farmers’ decision-making as individuals and members of a given community may become easier and more precise than without.
&nb...
Bao-Ming Ge* Dai-Zhen Zhang, Qiu-Ning Liu, Sen-Hao Jiang, Jun Cui, Chun-Lin Zhou and Bo-Ping Tang
... correlation coefficient analysis of individuals’ data indicated that there were significant correlations of abundances distribution among seasons (P < 0.050). The distribution of A. vulgare varied among different forests, and then the environmental change according to the dike age increasing should be considered as the main factor explaining such variation.
...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...nfection for biochemical analysis. The Albumin and total protein values were significantly low (P<0.05) in infected groups A and B as compared to control groups C and D on 5th and 7th day. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly (P<0.05) high in infected groups A and B. On 3rd, 5th and 9th day five birds from each group were slaughtered and histopath...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad

 

...tructural and functional analysis of mutated protein was anticipated by bioinformatics tools, which manifest mild changes in overall charge but altered post translational modifications in a way which might have a deleterious effect on ion channels anatomy and on the whole, pave ways to the genesis of cataract.
...
Fariha Imtiaz1, Muhammad Islam1, Hamid Saeed2,*, Bushra Saleem1, Maryam Asghar1 and Zikria Saleem2
...n methods. Phytochemical analysis of leaves was performed on powder and extracts of leaves using already reported methods. Mineral analysis was done using atomic absorption spectrophotometer. Hair growth promoting effects were examined using alopecia mouse model. Phytochemical analysis demonstrated the presence of higherquantities of carbohydrates, proteins and secondary metabolites in met...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...ignment and phylogenetic analysis exhibited that genes derived from the same GST class from different species share more similarity than genes of different classes in the same species. Copy number of GSTs examining showed that five classes of GST genes in common carp have undergone the gene duplications, including MGST1, GSTK, GSTM, GSTA and GSTT. Comparative genomics and syntenic analysis provided new evidences for better u...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1Nisar Ahmad3, Ayesha Hassan1, Sultan Ayaz2, Sajid Umar4, Muhammad Luqman Sohail5,*, Muhammad Shafee6, Ikramullah Khan7, Mumtaz Ali Khan1, Azmat Ullah Khan8Naimat Ullah Malik1 and Abdul Razzaq9
...ch district. Statistical analysis revealed significant difference (P<0.05) among children of different age groups with highest disease prevalence in children of less than 5 years of age (21.40%) and lowest in children of 11-15 years of age (15.99%). Cryptosporidium was significantly higher (P<0.05) in study subjects having livestock animal contact and persistent diarrhea, highlighting its zoonotic potential from livestock animals to human beings i...
Mutassim M. Abdelrahman1,*, Riyadh S. Aljumaah1, Moez Ayadi1 and Shabana Naz2
...um yield and biochemical analysis at parturition was also determined. A significant (P<0.05) increase of calcium (Ca), Zn, Co and Se levels in blood of ewes treated with bolus with a significant increase in phosphorous (P), Co and Se in the blood of their newborn lambs. Furthermore, a significantly higher (P<0.05) inorganic matter percentage in colostrum was found in treated group compared to the control. The body weight of lambs in treated group at 30 a...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...ere sequenced. In-silico analysis was performed to detect the possible disease causing effect of genetic variants with OCD. Sequencing of BDNF gene showed one change G>A at position c.196 (rs6265) resulting in a nonsynonymous change from valine to methionine. We found only one homozygous and heterozygous carrier for this polymorphism. Healthy siblings of the carrier individuals and control group were screened and found as negative for this polymorphi...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
... RNA secondary structure analysis was performed and SNP was found changing the termination loop shape in mRNA. However, to increase the selection efficacy of dairy animals, the effects of candidate polymorphism should further be screened in different environments and genetic backgrounds.
...

Naveed Muhammad, Syed Attaullah Shah*, Abbas Ullah Jan, Irfan Ullah, Muhammad Ibrahim and Salman Khan

...d. Allocative efficiency analysis reveals that machinery, seed quantity, number of irrigation, fertilizers and chemicals are underutilized, while labor and FYM are over utilized for tomato production in the study area. The study recommends training for growers and field labors, provision of short-term formal credit and subsidies on seed, chemical spray, fertilizers and other underutilized inputs/ capitals. 

...

Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3

...ntioxidant enzymes assay analysis. The data showed that as the citrus waste percentage in the feed of the broiler was increased, its feed intake and the body weight decreased but the citrus waste has non-significant effect on the feed conversion ratio of the broiler birds. The results also showed that citrus waste in the feed have positive effect on the antioxidant status of the broiler birds. The activity of the superoxide dismutase, catalase and glutathione ...
Wu Yi-qun* and Xu Xiu
...30 to 14:00. Correlation analysis on the wintering behaviors shows that there is a significant positive correlation (P < 0.05) between feeding and walking, air movement and chasing behavior, and the positive correlation between lying and preening behavior is also significant. The results also showed that weather conditions has obvious influence on wintering behavior, especially on feeding, walking, lying and standing (P < 0.01).
...
Muhammad Imran Shabbir
...ll as performing genetic analysis including prediction of patterns of inheritance in family lines and to calculate the recurrence risk for relatives etc. Punnett square is used to describe the possible combinations of paternal and maternal alleles for a particular cross. Described here is a line-dot method using ternary genotype codes to determine genotype probabilities in a very quick and simple way. Also ternary genotype codes along with gender symbols and a...
Hassen Allaya1,2,*, Abderraouf Ben Faleh1, Manel Rebaya1, Sonia Zrelli3, Abdallah Hattour2, Jean-Pierre Quignard4 and Monia Trabelsi1
...vestigation based on the analysis of morphological characteristics of Euthynnus alletteratus from two distant sampling sites (Mahdia and Zarzis) along the Tunisian coast using 11 morphometric and 3 meristic characters in order to investigate the morpholgical variation among them. Uni and Multivariate analysis of variance of 64 adult specimens showed significant differences among the means of the two studied sites for ...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and  Kui Chen2,*
...tion with bioinformatics analysis so as to understand the possible functions in response to chemical sensation behavior of taste receptors. The receptors when compared with T. rubripes, there was 99.2% identity in two kinds ORF sequences, with only 7 bases different, and there were 5 amino acids different between speculated fusion proteins. Under bioinformatics analysis, the T2R1 gene without introns was consis...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
... phylogeny and diversity analysis. In Pakistan hog deer (Axis porcinus) is an important wild species belongs to the family Cervidae. The hog deer is one of the least studied species at molecular level in Pakistan. The present study was planned to investigate its molecular phylogeny and diversity analysisusing mitochondrial cytochrome b, cytochrome c and d-loop region. Samples werecollected from di...

Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4

...lar assays. The sequence analysis of NA genes shown 99% homology with the H9N2 AIV recently isolated from Pakistan and their phylogenetic analysis revealed that all the isolates belonged to the G- lineage. Amino acid analysis of NA stalk regions of these isolates illustrated no insertion, deletion and substitutions compared to A/DK/HK/Y280/97 lineage and human isolates (A/HK/1073/99 and A/...

Sania Shaheen1*, Hina Fatima2 and Muhammad Azeem Khan3

...ala. Stochastic frontier analysis (SFA) was applied to find the results of a study. Study results reveal that direct seeding method is more profitable for dry rice farmers in terms of yield and also increases the efficiency of farmers. Area under rice crop, NPK ratio, Seed, number of irrigation hour, weedicides, insecticides and pesticide variable would significantly contributes to improve the rice production. On average technical efficiency of sample rice far...
Fareeda Tasneem1, Farah Rauf Shakoori1 and Abdul Rauf Shakoori2,*

 

...a molecular phylogenetic analysis was performed on the basis of H4 gene sequences. A phylogenetic tree was constructed using the aligned sequences of our study with 22 entries of closely related species from GenBank Data. Highly polymorphic sites were observed in FT8 strain sequence as compared to other species. Analysis of H4 gene sequences in our study showed they are closely related and behaved as a good substitute for ph...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
Ibrahim A. Alhidary1,*, Mutassim M. Abdelrahman1, Riyadh S. Aljumaah1, Abdullah H. Alyemni2, Moez A. Ayadi1,3 and Mohamed Y. Al-Saiady2
... for different metabolic analysis. At the end of the trial, six lambs from each group were randomly selected and slaughtered for rumen coloring. The results of this trial showed a significantly higher total body weight gain in lambs fed TMR1 and TMR 2. A significantly higher FCR in lambs fed TMR3 compared with all other treatments. The rumen color of lambs fed TMR2 was significantly (P < 0.01) lighter compared with all other groups except lambs fed A...

Fawad Ali1*, Hidayat Ullah2, Ikhtiar Khan1

...ities. Linear regression analysis indicates that concentration of the heavy metal (Pb, Cr, Cd, Ni, Zn) in vegetables are significantly correlated at R2 value of 0.956 at P≤0.001 with their extractable concentration in soil. Health risk index (HRI) has been found>1 for Pb in Abelmoschus esculentus while rest of the heavy metals have been noted less than 1 indicating relative absence of risk concerned with ingestion of adulterated vegetables. This study hi...
Omar S.O. Amer1,3,*, Mohamed I. Waly2,4 andSaeed A. Al-Zahrani1
...>This is a retrospective analysis of reported intestinal parasitic infections for patients visiting Prince Sultan Military Medical City, Riyadh, KSA from 2010 to 2014. Our retrospective study estimated a total 775 case out of 11110 case were infected with one or more intestinal parasites, with prevalence rate 6.98 %. The prevalence of intestinal parasitic infection during the period of study was as follows; Ascaris lumbricoides (n= 205, 1.8%), Giardi...
Saleem Khan1, Amir Hamza2, Farhadullah Khan3, M. Subhan4, Aziz Khan1, Irfan Ali Shah1, Shakirullah Khan Shakir3*
...align: justify;">For the analysis of gamma rays-induced changes in different growth related end point using cotton as a model plants, the present study was designed. For this purpose, dry seeds of four cotton genotypes (Gomal-93, Bt-131, Bt-121 and Bt-CIM-602) were exposed to gamma rays with 10, 15, 20 and 25 Kilo Radium (KR) doses sourced from 60Co. The irradiated seed samples were assumed treated seeds while non-irradiated seeds of each genotype w...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,
...rincipal Coordinate analysis. These polymorphic markers will be useful tools for investigating genetic diversity and population structure of this species.
...
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
... theCOMT gene. In-silico analysis predicted it to be damaging and disease causing. Screening of case and control group data showed no deviation from Hardy-Weinberg equilibrium with p-value 0.879 and 0.32, respectively. There was no significant difference in age (p-value 0.081), employment status (p-value 0.34) and matrimonial status (p-value 0.28) but there was a significant difference in their education (p-value 0.0002). The...
Ambreen Butt1, Uzma Malik2, Khadija Waheed3, Amna Khanum3, Samar Firdous4, Sara Ejaz5 and Fawad Randhawa2, Tania Shakoori6*
...and gravidity. ROC curve analysis was conducted to assess the suitability cobalamin as a predictive biomarker for GDM. Median cobalamin were significantly reduced in cases (108.95±92 pg/mL) as compared to controls (173±90 pg/mL) at p<0.001. Low vitamin B12 levels (β = -0.29, p=0.004) significantly predicted high fasting blood sugar levels in pregnancy even after adjusting for gestational age and gravidity. ROC curve ...

Carolina Torres Alejo1,2, Laila Andreia Rodrigues Beserra1,2, Paulo Eduardo Brandão1,2, Luis Ramiro Luna Espinoza1,2 and Fabio Gregori1,2* 

...ucleotide sequencing and analysis. Twenty-nine pools (29/59; 49%) appeared positive for AAstV, 6 pools (6/59; 10%) were positive for RVD, forty-three pools (43/59; 72.8%) were positive for ACoV, and twenty-four pools (24/59; 40.67%) presented concomitant viral infections of two or three investigated agents. The relative high frequency of occurrence of the studied viruses both in single or concomitant infection emphasizes the need for continued monitoring of po...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
...parvas. Risk factors analysis namely housing type, tick control, age and sex of animal presented significant (P <0.05) association with tick infestation and burden while type of breed showed significant association with tick infestation but was non-significant with tick burden. Topography presented inverse behavior to that of breed with tick burden and tick infestation. On the other hand, geo-location was only factor exhibiting non-significant ly associ...
Rana Muhammad Aadil1,2*, Xin-An Zeng1, Saqib Jabbar3, Akmal Nazir2, Abid Aslam Mann2, Muhammad Kashif Iqbal Khan2, Abdullah2, Amna Ramzan2
...icroorganisms and enzyme analysis. The grapefruit juice was treated with processing variables of HPP treatment (0, 150, 200 and 250 MPa), temperature (40, 50 and 60 oC), for processing time of 3 minutes and thermal treatment was done in a water bath at 70 oC for 90 seconds. In HPP treatment (250 MPa/60 °C/3 min), significant reduction was observed in microorganisms, PME and PPO whereas improvement was observed in cloud value, total ca...
Masooma Munir1,2*, Aqsa Qayyum1, Saeeda Raza1, Nouman Rashid Siddiqui1, Amer Mumtaz1, Naeem Safdar1, Sahar Shible1, Sohaib Afzal3, Saiqa Bashir4
...rink. Proximate, mineral analysis, total polyphenol content and mineral analysis of basil seeds was conducted. Result revealed that basil seeds are not only good source of fiber and protein but they provide appreciable amount of minerals and phenolic compounds. Swollen basil seeds were used to prepare beverage at three supplementation levels i.e. 0.2, 0.3 and 0.4%. Sensory evaluation of basil seed drink revealed that 0.3% ba...
Jie Wu1, Jiagui Zhu2, Ke Wang2, Dejing Cai2, Yingying Liu1, Yanzhen Bu1,* and Hongxing Niu1,*
... the principal component analysis (PCA) revealed that the first four components accounted for 79.31% of the total variance, including vegetation, interference, nest position and food factors, which best reflected the habitat site selection of the crested ibis. The crested ibis were more likely to choose higher tree nests that were closer to residential areas and in a lower slope position in the breeding season; 2) thirty-three eggs were laid in 12 nests, the a...
Nargis Bano and Khalid Mahmood Qureshi*
...UR Pakistan. Statistical analysis showed drought stress significantly reduced vegetative and reproductive growth, along with various and biochemical attributes, but augmented the leaf proline. SA application significantly increased plant fresh and dry weight, leaf area, proline and decreased root length, number of roots and water use efficiency. SA treatment therefore decreased adverse effect of stress on strawberry plants. Thus the result suggests t...
Aqsa Mehboob1, Noor Khan1,*, Usman Atiq1, Khalid Javed Iqbal2, Rafia Tayyab1, Syeda Suhaira Batool1, Hafiza Saleha Batool1, Sana Amjad1 and Mehwish Tanveer1 
...T1. Proximate analysis revealed significant differences (P≤0.05) among treatments and control for crude protein while rest of the parameters remained same. Apparent digestibility of crude protein and fat was found non-significantly different among treatments. It is concluded that addition of fenugreek in fish feed can improve growth performance and also protein content of the fish meat as well.
...

Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Wasiullah1 and Wiqar Ahmad

...ue and post-harvest soil analysis. But in this paper, data on availability of major plant nutrients, soil organic matter and plant dry weight are presented. The data revealed that cyanobacteria strains had significantly (p < 0.05) increased the availability of major plant nutrients and SOM under the saline soil conditions. Among the strains, Gloeobacter MMF-4 significantly increased the soil organic matter (SOM), nitrogen (N), phosphorus (P), potassium (K),...

Hassan Hashim Ghalib, Syed Attaullah Shah*, Abbas Ullan Jan and Ghaffar Ali 

... descriptive statistical analysis demonstrate that net-return per acre from wheat crop is highest in the Central zone because of conducive agro-climatic conditions. The estimated Cross-sectional Ricardian Model reveals that temperature and rainfall have non-linear relationships with net-return per acre. The relationship between growers’ net-return and atmospheric temperature, represented with a temperature response curve, is inverted U shaped having a yi...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...gene. After phylogenetic analysis with 3 clonal types and atypical strains, and on the basis of restriction map of HhaI and Sau3AI, our 3 isolates of T. gondii were found more closely linked to a typical strain. This is the first genetic analysis of T. gondii in Pakistan. In order to develop our knowledge about the toxoplasmosis epidemiology, further genotype analyses of T. gondii from animals and man ne...
Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem
...kers, we show a sequence analysis of (V3, V4 and V9) hyper-variable regions of the small sub unit (SSU) of different natural strains of the Pyrenomycete, Sordaria fimicola, to examine genetic diversity. Strains were taken from two contrasting environments (Natural and Harsh) from the south facing and north facing slopes of Evolution Canyon, Israel. Sequenced amplicons were introduced to the NCBI database as a query in BLASTN. The query revealed >100%...

 Zabih Ullah*, H. Rahman and Niaz Muhammad

Akerele Dare*, Ashaolu Olumuyiwa Fowowe, Sanusi Rahman Akintayo and Egbetade Adedolapo 

...onometrics (time series) analysis. Results established positive and statistically significant influence of the amount of fund apportioned to crop (p<0.01) and livestock (p<0.05) subsectors on output growth in the respective subsectors. Likewise, the amount of the fund allocated to fishery subsector on fish output has positive influence on fish output but significant only at 10% level. While increases in the amount of the fund in a given year may substant...
Nosheen Rafiq1, Asmatullah Kakar2,*, Arshad Ghani3, Asim Iqbal2, Wali Mohammad Achakzai2, Shagufta Sadozai2, Mohammad Shafiq4 and Mohammad Alam Mengal5 

Arshad Iqbal1*, Iftikhar Hussain Khalil1, Syed Mehar Ali Shah1 and Muhammad Sharif Kakar

...ated traits. Correlation analysis showed that grain yield had strong positive phenotypic and genetic correlation with heading, maturity, flag leaf area and biological yield. Spring wheat genotype SM12 had best performance for grains spike-1 and grain weight spike-1 should be further tested at different location of Khyber Pakhtunkhwa for possible release as a new cultivar.  

...
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
... infection. Based on the analysis above, we speculated that BmARM-like is involved in response against BmNPV infection.
...
Mubashir Hussain1,Safdar Ali1, Muhammad Naveed Tahir1, Ghulam Abbas Shah1, Ijaz Ahmad2*, Muhammad Aqeel Sarwar3 and Sohail Latif4
...s for the weed seed bank analysis were taken from the experimental field before wheat sowing from 0-10 cm, 11-20 cm and 21-30 cm soil depth. Two soil weed seed bank determination techniques were compared viz., sieving method and seedling emergence method. The data were collected on the seed density m-2, seed frequency, diversity of weed species and relative importance value. The input and output data was also collected to find out the socioeconomic ...
Tahira*, Muhammad Arshad, Muhammad Ayub Khan and Mubashar Ahmad Khan
...n 2014 and 2015. Cluster analysis based on WARD’s method showed considerable genetic variation among the hybrids. In the first year, thirty- six Brassica hybrids were grouped into thirteen clusters. Cluster-IV comprised of maximum number of hybrids (six). The clusters mean and standard deviation marked that hybrid in Cluster XI (CRH-80) was short durational and high yielding mainly due to more branches and pods per plant.CRH-35 in cluster-VI was high yie...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
...d using RT-PCR. Sequence analysis demonstrated significant differences between amino acid sequence of C. dromedarius and other mammalian GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that CdGSTP1-1 is grouped with the enzyme from S. scrofa. The 3D homology model of CdGSTP1-1 showed similar fold and topology with the porcine GSTpi enzyme. Gene expression
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...d by immunohistochemical analysis showed either weak (the black cashmere goat) or strong (the white cashmere goat) expression of the KIT protein. These findings suggested a relationship between mutations in KIT exon 13 and differential fur color in cashmere goats. These results lay the foundation for further research on exon 13 of the KIT gene and color regulation in cashmere goats. 
...
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...escence quantitative PCR analysis revealed that the native breed (Rongchang pig) has a better resistance to disease than Landrace. When Rongchang pig and Landrace were 0 day old, the expression level of BPI of Rongchang pig was higher than Landrace in heart, liver, spleen, kidney, small intestine, lower than Landrace in lung. When Rongchang pig and Landrace were 28 day old, the expression level of BPI of Rongchang pig was higher than Landrace in spleen (p
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*

Ehtisham Shakeel Khokhar1*, Amir Shakeel1, Muhammad Amir Maqbool1, Muhammad Waheed Anwar1, Zoraiz Tanveer1 and Muhammad Fahad Irfan2 

Guang-Xin E1, Yong-Ju Zhao1, Yue-Hui Ma2, Ming-Xing Chu2, Jia-Hua Zhang1, Zhong-Quan Zhao1, Hui-Jiang Gao2, Huai-Zhi Jiang3, Di Liu4, Li Liu5, Yan-Bin Zhu6, Wang-Dui Basang6, Luo-Bu Danjiu7, Tian-Wu An8, Xiao-Lin Luo8, Shi-Cheng He7 and Yong-Fu Huang1,*
... categories, and pathway analysis revealed that these DEGs were significantly enriched in 41 of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including signaling pathways regulating the pluripotency of stem cells, the p53 signaling pathway, and oxidative phosphorylation. The results of the present study confirmed that the selection of the dominant follicle involved the regulation of various physiological systems. These results provided helpful d...
Jie Wu1, Jiagui Zhu2, Ke Wang2, Dejing Cai2, Yingying Liu1, Yanzhen Bu1,* and Hongxing Niu1,*
... the principal component analysis (PCA) revealed that the first four components accounted for 79.31% of the total variance, including vegetation, interference, nest position and food factors, which best reflected the habitat site selection of the crested ibis. The crested ibis were more likely to choose higher tree nests that were closer to residential areas and in a lower slope position in the breeding season; 2) thirty-three eggs were laid in 12 nests, the a...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...ere subjected to one way analysis of variance. High sperm individual motility (85.12 ± 0.51%), progressive motility (20.25 ± 0.47%), plasma membrane integrity (82.25 ± 0.43%), acrosomal integrity (79.91 ± 0.51%), and DNA integrity (98.54 ± 0.14%) were observed in fresh semen in Sahiwal bulls, which were significantly (P < 0.01) higher compared with the other cattle bulls. High dead sperm percentage (51.87 ± 0...
Quang Minh Dinh
...h-frequency distribution analysis of 1,527 individuals. The male to female T. vagina ratio was not significantly different within and between seasons, and the parameters of the von Bertalanffy curve of this fish were given as L= 24.15 cm, K = 0.53 yr-1 and t0 = –0.03 yr-1. The longevity (tmax) and the growth performance (Φ’) were 5.56 yrs and...
Anan Kenthaoand Pornpimol Jearranaiprepame*
...l analyses. Multivariate analysis of variance (MANOVA) indicated highly significant differences in morphometric characters between populations (p <0.01). The first three principal axes of principal component analysis (PCA) explained 49.29% of total variance. The PCA also revealed that morphological variations related to the characters of head depth, body length, body depth, caudal peduncle length and depth. In disc...
Rana Manzoor Ahmad1,2, Abdul Majid Khan1,*, Ghazala Roohi3 and Muhammad Akhtar1
...esent study involves the analysis of enamel hypoplasia in seven extinct giraffid species to determine and compare stress periods during the early Miocene to Pleistocene ages of the Siwaliks of Pakistan. Enamel hypoplasia is a tooth malady which is caused by the deficiency of food/nutrients. The feeding deficiency is directly linked to the physiological or environmental stress. In this study occurrence of enamel hypoplasia in giraffids has been observed in spec...
Hafiz Muhammad Ishaq1, Muhammad Shahzad2, Xiaokang Wu3, Chaofeng Ma4 and Jiru Xu1,*
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...ein-Friesian cattle. The analysis included 1024 cows and 3663 calves of the Polish Black-and-White Holstein-Friesian breed (phf). The study included an analysis of one polymorphic place PIT-1 (c.1178G>A). The source data used to calculate the indicators of reproduction were collected from cows inseminated by semen of Black-and-White Holstein-Friesian bulls and giving birth to single calf. The following information was tak...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
.... Thereafter, comparison analysis was done on miR-122, miR-449a, DUSP4, PDK4 and FKBP1B mRNA expression levels in different bull testes tissues (1-day, 12-months-old and 24-months-old). In addition, genes mRNA and protein levels were detected after bull testicular Sertoli cells were transfected with miR-122 and miR-449a. Meanwhile, DUSP4, PDK4 and FKBP1B overexpression experiments were conducted. Eventually, MTT assay ...

Safdar Ali1*, Ghulam Abbas Shah1, Muhammad Naveed Tahir1, Shahid Mehmood2, Asim Gulzar3 , Ijaz Ahmad4 and Bashir Ahmad Khan5 

...P + 4 CS. The regression analysis proved that soil moisture content and bulk density have a great association with tillage systems, whereas, the correlation analysis proved that soil porosity and tillage intensity have a strong and positive correlation with wheat grain yield. In conclusion soil moisture dynamics can be improved by combine use of no-till and glyphosate herbicide (NT + GH)while soil porosity, its productivity ...

Gamalat Osman1, Mansour Galal1, Abbas Abul-Ezz2, Ahmad Mohammed1, Mohammed Abul-Ela3 and Asmaa Mostafa Hegazy2

...inatus was 700 ppm. HPLC analysis of 16 polycyclic aromatic hydrocarbons (PAHs) in the flesh demonstrated that PAHs accumulation in the treated snails was fluctuated with time. The accumulation is due to their biotransformation process during metabolism. The histopathological observations of the gills and mantle of L. carinatus showed pronounced alterations at 24 hours and proliferated at the day 15 but showed a further decline at the day 30 of exposure. These...

Manzoor Hussaina,b

...ere saved for subsequent analysis of Y-STRs. It was found that autosomal DNA profile of semen donor up to TSI 48 hours is feasible; however, chances of getting complete male DNA, partial male DNA profile and no male profile at TSI 72 hours are 17%, 67% and 16% respectively. Chances of getting complete male DNA profile becomes zero at TSI 96 hours, however, partial male DNA profile is accessible (40%) at TSI 96 hours. It is concluded that autosomal DNA STR syst...

Malak Atiq Ullah Khan* and Fida Mohammad 

...grain yield. Correlation analysis revealed significant positive association of grain yield with tillers m-2 (rg = 0.72**), grains spike-1 (rg = 0.41**) and 1000-grain weight (rg = 0.30**). Based on mean yield performance, G-79 was found as high yielding genotype and thus could be recommended for commercialization in Khyber Pakhtunkhwa. 

...

Sajid Muhammad* and Fida Mohammad 

...2 and 2012/13.GGE biplot analysis explained 79.89% (PC1=56.57 and PC2= 23.32%) of the total variation. Genotype G19 positioning on vertex in sectorE-3, E-4 and E-5, while G30 in sector E-1 and E-2 revealed their specific suitability to these environments. GGE biplotrecognizedE-4 as ideal environment, whereas G49 as ideal genotype.Thus, GGEbiplotanalysis identified G49 (Wafaq×Ghaznavi-98-3) as high yielding stable genot...
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Sara Ferrando4 and Lorenzo Gallus
...rvival rate. Biochemical analysis of fish meat showed that moisture, protein, lipid, ash and crude fiber were not significantly (P>0.05) affected by salinity level. The hematological parameters like hematocrit, cholesterol and plasma triglycerides were similar among fish fed on different salinity level (P < 0.05).
...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...of Pakistan. Statistical analysis suggested predicting variables including “local equine density per 2 KM”, “equines workings in ponds and rivers”, and “geography of the equine” were significantly (P<0.05) associated with seroprevalence of EIV. Our findings of the EIV occurrence in equine population in Pakistan, suggests an under-diagnosis of this virus in Pakistan and warrant additional investigation and continuous surve...
Yunjun Yan, Zhenming Lü, Tianming Wang, Yongjiu Chen, Jingwen Yang, Baoying Guo, Lihua Jiang, Changwen Wu and Liqin Liu*
... this LNCR. Phylogenetic analysis of 24 cephalopoda species based on the complete mitochondrial genome showed that the O. dollfusi is most closely related to Amphioctopus aegina. These results seems to support the recent notion that O. dollfusi should be considered as synonym of Amphioctopus aegina. More morphologic and molecular evidences should be involved to resolve the taxonomic status of O. dollfusi in future’s stu...
Muhammad Usman Saleem1, Saima Masood1,*, Hafsa Zaneb1, Aneela Zameer Durrani2, Asim Aslam3, Kamran Ashraf4, Habib-ur-Rehman5, Muti-ur-Rehman3 and Muhammad Shabir Shaheen6
...ate were slaughtered for analysis of the selected parameters. The results showed that carcass weight and carcass yield were significantly high (p < 0.05) in MOS+OAB compared to MOSG, OABG and CONT. The results of small intestine microarchitecture showed that villus length, villus width, villus to crypt ratio, villus surface area, acidic, mixed and total goblet cells increased significantly (p < 0.05) in birds that were fed MOS and OAB alone and in combin...
Abd El-Nasser Ahmed Mohammed*
...d samples were taken for analysis of complete blood picture. The results indicated presence of a neovascular capillary network around transplanted ovarian tissues. In addition, visible antral follicles and corpora lutea was demonstrated on the ovarian tissues upon transplantation. Histological examination indicated that transplanted young ovarian tissues restored function better than elder ones. It could be concluded that unilateral ovarian transplantation par...

Waqas Liaqat1, Mohammad Akmal1* and Jawad Ali 

...ed bed preparation. Data analysis showed that emergence m-2 affected by varieties only. Both crop phenology (i.e. days to tasseling, silking & maturity) as well as morphology (i.e. plant height, ear height, including ear length) was affected (p<0.05)by sowing dates. Likewise, yield traits (i.e. rows per ear, grains per ear, thousand grain weight) were also adversely affected by sowing dates, which decreases both biomass and grain yield. Varieties did di...

Syed Asif Imran Shah1*, Shah Jehan Khan2, Kalim Ullah1 and Obaid Ullah Sayal

...sing principle component analysis. The experiment was carried out at Cotton Research Station, Deara Ismail Khan, Pakistan during 2014-15. The triplicated randomized complete block design was followed and attributes of interest were plant height, monopodia plant-1, sympodia plant-1, bolls plant-1, plant population, seed index, boll weight, lint index, staple length, lint %, fibre fineness,staple strength, uniformity ratio and seed cotton yield. The

Muhammad Luqman1,3, Raheel Saqib2*, Xu Shiwei3 and Yu Wen3 

...d were coded on SPSS for analysis and interpretation. Results showed that there is significant difference in age and educational status of male heads and their spouses. Majority of the farm families (39.3%) earn income for their livelihoods both from farming and non-farming sources. Intensity of participation of female respondents in different crops and livestock activities was comparatively high as compared to their male counterparts. It was found that averag...

Wang Jian1, Abdur Rehman2*, Noor Khan3, Wang Zhengjia4, Ji Peng-fei5, Xie Cong6, Muhammad Niamatullah Khan Babar7 and Raheel Saqib8 

... descriptive statistical analysis, correlation test, Extend Linear Expenditure System (ELES) model, Gray relational analysis and the residual test have been used in this study.The results of the study indicates that all the rural households consumption have the increasing trend except the Engel coefficient shows the declining trend from 39.508% to 29.355%.The study of regression analysis, ...

Afsheen Masood1*, Rubab Musarrat2, Shama Mazahir3 and Adeela Ashraf4  

...cated by the correlation analysis that better greater satisfaction was associated with better quality of life. The findings carry significant implications for clinical professionals, health psychologists, community workers and the future researchers. 

...

Aneeza Jamshed1* and Syed Amir Gillani2 

...l 2014 to Sep 2014. Data analysis was done with SPSS 20. After describing the important variables, average percentages were used to find out the difference between pre and post awareness performances and t score and p value was calculated. All the students (150) were boys, 98% belonged to a lower middle class family with the age of 9-12 years. During pre-testing evaluation, students were never given the knowledge about hepatitis C, its source of transmission (...

Arooj Fatima1* and Ashfaq Ahmed

...0) when repeated measure analysis of variance (ANOVA) was applied comparing four sub groups. Eccentric exercises give better results in reducing pain intensity and gaining functional outcome with routine physiotherapy maneuvers in subjects having rotator cuff tendinopathy. Eccentric loading training for the cure of shoulder pain due to rotator cuff tendinopathy has confirmed to be more helpful in aiding pain, and fixing functional impairment in patients having...

Muhammad Yahya Noori1*, Saharish Rizwan2, Zaheer Ali3 and Shaheen Sharafat4 

...culosis. Tabulations and analysis were performed in Microsoft Excel® and online calculators. Analysis of contact data showed that only 36% of the patients had a close contact with a known Tuberculosis patient. Chi-square test did not show any association between history of presence of a contact and the patients’ gender (p>0.5). Among the cases with established exposure with a TB patient among close contacts, 17....

Bashir Ahmad1*, Ahmad-Ur-Rahman Saljoqi1, Hayad Zada2, Shahid Sattar1, Toheed Iqbal3, Saddam Hussain1 and Muhammad Saeed4 

... association. Regression analysis showed 87-96% (R2) variability due to weather parameters on the population dynamics of P. xylostella at District Haripur. 

...
Justyna Batkowska1,*, Lukasz Wlazlo2, Kamil Drabik1, Bozena Nowakowicz-Debek2, Karrar I.A. Al-Shammari3 and Magdalena Gryzinska1
...les of eggs to microbial analysis were. After 17.5 days of incubation its results were evaluated. Chicks were reared for 14 days and their livability and body weight gains were registered. The effectiveness of grapefruit juice as a disinfectant for hatching eggs was noticed in terms of reducing the development of microflora on the eggshell. Its use did not directly affect the hatching results and chick quality, however, they were similar to control groups, wha...
Muhammad Aqeel Sarwar1*, Abid Ali2, Manzoor Hussain2, Saadia3, Muhammad Khubaib Abuzar4, Ijaz Ahmad5 and Sohail Latif6
... F2, economic analysis showed that maximum net income and benefit cost ratio were obtained where (F3)75% recommended dose of NPK + 1.5 t ha-1 poultry manure was applied. Therfore, it can be concluded that 75% recommended dose of NPK + 1.5 t ha-1 PM along with Azospirillum seed inoculation could be a better management approach for sustainable wheat production.
...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
.... The spectrophotometric analysis at 474nm of extracted color both in crude form and in candies was found to be stable at 4oC and showed decay in mean lutein concentration at higher temperatures i.e. 25oC and 45oC. The microbiological study of the extracted color and candies prepared in laboratory and dyed with T. erecta extract color showed that the color extracted from T.erecta itself do not promote microbial gr...
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...d PCR-restriction enzyme analysis. Mycobacterium bovis (M. bovis) and M. tuberculosis H37Rv were used as reference strains. RAPD-PCR differentiated the strains and authenticated the high molecular diversity. PCR-restriction enzyme analysis (PRA) profiles of all isolates and reference strains were found to be the same. PRA is a valid alternative to phenotypic identification in a routine diagnostic ...

Ayesha Saeed*, Mobina Naqvi and Ahsan Javed 

...96, p=0.002). Regression analysis indicated a significant association (AOR 16.076, CI=2.381-108.564, p=0.004).Based on these studies, it was concluded that food insecurity in pregnancy is associated with low birth weight in neonates. Antenatal screening of food insecurity and timely provision food supplements through social welfare can help reduce neonatal morbidity and mortality. 

...

Zahid Akram, Saif Ullah Ajmal, Ghulam Shabbir, Muhammad Munir and Nasir Mahmood Cheema

INHERITANCE MECHANISM OF SOME YIELD COMPONENTS IN BREAD WHEAT
...grain yield plant-1. The analysis of variance depicted that 56 F1 and their parents were significantly different for all the parameters. Uniformity of Wr-Vr over arrays as well as significant deviation of regression coefficient (b) from unity confirmed the suitability of additive-dominance model to account for the data analysis. The estimates of genetic component of variation in all the traits exhibited the preponderance of<...

Sidra Majeed, Shahid Ahmad*, Asif Ali Bhatti** and Ghazanfar Abbas***

...nd 1.0 t ha- 1. Economic analysis revealed that the average net return was Rs. 6744 ha-1 with minimum loss of Rs. 7674 ha-1 during 2000-01 to a gain of Rs. 20822 ha-1 during 2004-05. The analysis of cost of production indicated that the increase was largely in the cost of tillage and harvesting due to increase in the price of diesel fuel and renting cost of farm machinery. The average net return from barani wheat using chemi...

 Babar Hussain*, Muhammad Ashfaq**, Mazher Abbas***, Khalid Mahmood**** and M. Ather Mahmood***

...tan using co-integration analysis. Co-integration results show that all gram markets are highly co-integrated in the long run. The high degree of market integration observed in this case is consistent with the view that Pakistan’s gram markets are quite competitive and provide little justification for extensive and costly government intervention designed to improve competitiveness to enhance market efficiency.

...

 Sumia Bint Zaman, Sidra Majeed and Shahid Ahmad*

...t a comparative economic analysis of alternate feasible options e.g. production of oilseeds, which are also being imported in large quantities. Temporal analysis (1950-09) for edible oil consumption, production and imports is made. Projections for edible oil are worked out up to 2030. As there have been large variations in yield of Jatropha reported by various studies conducted in India and other countries, therefore most re...

 Shazia Erum, Rashid Anwar and Shahid Masood*

...esent study, comparative analysis of total seed proteins of Kasuri methi (GI of Kasur, Pakistan) was evaluated with other Trigonella genotypes by SDS-PAGE. Results showed that at protein level Kasuri methi acquired a unique status as a G.I of Kasur. Cluster analysis (UPGMA) of 28 genotypes including both methi and methray from various agro ecological zones of Pakistan were interlinked to some extent however Kasuri methi make...

 Rubina Bano, Hassnain Shah, Muhammad Sharif and Waqar Akhtar*

...district Rawalpindi. The analysis covers the descriptive analysis of socioeconomic characteristics of the sample poultry farmers along with cost and profitability analysis. Broiler farming was done by adult males on full time basis. It was the main source of income of a family. The average flock size in the study area was 4033 birds. The study also bifurcates the cost structure and fixed c...

Halima Shoukat1*, Hafiz Sheraz Arshad2, Faiza Sharif1, Arooj Fatima1 and Filza Shoukat

...pplying repeated measure analysis of variance (ANOVA), the p-values were found to be statistically significant (p<0.001) for static stretching techniques with 30s, 60s, 90s holding times, respectively. Based on these finding, it is plausible to conclude that application of static stretching at holding times of 30, 60 and 90 seconds can significantly improve the ROM of knee extension. Stretching techniques with different holding times were equally effective ...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...health and hematological analysis of Pangasianodon hypophthalmus (initialaverage weight 32.46±0.36 g). The 40% crude protein diet was prepared by adding feed ingredients along with 0.75, 0.50, 0.25, 0.00 g kg-1 of α-amylase as T1, T2, T3 and T4 (control). At the end of feeding trial, 100% survival rate was recorded. Growth rate was significantly increased in fish fed with enzyme supplemented diets in comparison with control group...
Mustafa Sahin1,*, Esra Yavuz1 and Fatih Uckardes2
...at the use of RR and PCR analysis methods could be more accurate instead of the LS method under multicollinearity problem.
...
Umair Riaz*1, Zafar Abbas1, Qamar uz Zaman2, Muhammad Mubashir1, Mahwish Jabeen2, Syed Ali Zulqadar1, Zeenat Javeed1, Saeed-ur-Rehman1, Muhammad Ashraf1 and Muhammad Javid Qamar1
...;
...

Imtiaz Hussain*, Hassnain Shah, M. Azeem Khan, Waqar Akhtar**, Abdul Majid*** and M. Yaqub Mujahid*

Corresponding author:izhussain@yahoo.com

PRODUCTIVITY IN RICE-WHEAT CROP ROTATION OF PUNJAB: AN APPLICATION OF TYPICAL FARM METHODOLOGY
... the system. Descriptive analysis is conducted on the data collected from 29 representative farms, from 10 Tehsils of four districts of rice-wheat in Punjab having 1-68 ha farm size selected through purposive random sampling applying typical farm methodology. Rice is cultivated on 87% and fodder on 8% of the total area during kharif while wheat on 75% along with berseem on 14% of total area during rabi season. Almost 81% rice and more than 50 % wheat is harves...

  Farhat Ullah Khan, Nowshad Khan and Zaibunnisa Abdullah*

Corresponding author: farhatkhan7@gmail.com (This is part of Ph.D. work of senior author)

IMPACT OFKINNOW (MANDARIN) YIELD LOSSES ON SOCIOECONOMIC CONDITIONS OF THE FARMING COMMUNITY IN PUNJAB, PAKISTAN
...techniques were used for analysis. According to analysis 91.5% respondents reported that yield losses of kinnow fruits have negative impact on socio-economic conditions of the majority respondents of the project area. Chi-square test also shows negative link of the kinnow fruits and socio-economic uplift of the farming community in the project area. Serious attention on scientific basis to yield losses through awareness and ...

 Muhammad Arifullah, Muhammad Munir*, Abid Mahmood**, Saifullah Khan Ajmal and Ghulam Shabbir*

COMBINING ABILITY ANALYSIS OF SOME YIELD ATTRIBUTES IN INDIAN MUSTARD (BRASSICA JUNCEA L.)
...em for combining ability analysis. Data was recorded for seed yield and some important yield attributes. The results for combining ability analysis indicated significant general combining ability for most of the traits except plant height and siliqua length. UCD-8/4, KJ-119 and BRS-2 were good general combiners for yield related traits. A cross BRS-2 × UCD-8/4 showed best desired SCA for number of primary branches and ...

 Faisal Khalil, Khalid Mahmood Qureshi, Ammaz Khan, Fakhar-ul-Hassan and Nabila Bibi*

EFFECT OF GIRDLING AND PLANT GROWTH REGULATORS ON PRODUCTIVITY IN OLIVE (OLEA EUROPAEA)
...eplications. Statistical analysis of data showed that T where GA was applied @ 30 ppm caused 2 3 more pronounced effect on number of inflorescence, flower count, perfect flower percentage, fruit drop percentage, fruit set percentage, fruit harvest percentage, fruit size, fruit weight, pulp weight and oil contents followed by girdling, NAA and SA. All growth parameters were found enhanced in Uslu (V ) with exception for fruit drop percentage, fruit size and fru...

 Parvez Khaliq*, Azim Malik**, Nasir Mahmood Cheema* and Muhammad Umair***

ECONOMICS OF WHEAT BASED CROPPING SYSTEMS IN RAINFED AREAS OF PAKISTAN
...opping systems. Economic analysis of the data revealed that the traditional wheat-fallow-wheat cropping system could be economically replaced with wheat-maize fodder-wheat cropping system even under drought condition and there will be no economical loss of wheat yield when planted after maize fodder. Application of recommended dose of fertilizer -1 along with FYM @ 5 tha will enhance the yield of wheat and maize fodder. The improved cropping system of wheat-ma...

 Gulzar S. Sanghera and Wassem Hussain*

HETEROSIS IN RELATION TO COMBINING ABILITY PER SE PERFORMANCE IN TEMPERATE RICE (ORYZA SATIVA L.)

 Saima Rani and Irum Raza*

COMPARISON OF TREND ANALYSIS AND DOUBLE EXPONENTIAL SMOOTHING METHODS FOR PRICE ESTIMATION OF MAJOR PULSES IN PAKISTAN
...ing methods namely trend analysis and double exponential smoothing. Measures of accuracy (MAPE, MAD and MSD) were used as the model selection criteria that could best describe the trend of prices of major pulses such as gram, mash, masoor and mung during 1975-76 and 2009-10. Double exponential smoothing method was found to be pertinent for price estimation of major pulses in Pakistan because of smaller values of accuracy measures. Six-year's forecasts of price...

 Muhammad Yaqoob*, Rukhsana Anjum, Mumtaz Hussain and Muhammad Jahangir Shah**

GENETIC DIVERSITY ANALYSIS AND CHARACTER ASSOCIATION IN SOME CHINESE HYBRID RICE UNDER DRY CONDITIONS
...recorded. The results of analysis indicated a high range of genetic variation among various hybrids for plant height, number of grains per panicle, number of productive tillers per plant, 1000 grain weight and paddy yield. The variation for days to 50% panicle initiation, panicle length, spikelet fertility and days to maturity were however nonsignificant. Plant height ranged from 77 to 116 cm, number of grains per panicle from 152 to 449, number of productive ...

 Arshad Ali, Muhammad Arshadullah, Syed Ishtiaq Hyder, Imdad Ali Mahmood and Badar-uz-Zaman*

RICE PRODUCTIVITY AND SOIL HEALTH AS AFFECTED BY WHEAT RESIDUE INCORPORATION ALONG WITH NITROGEN STARTER DOSE UNDER SALT-AFFECTED SOIL
... (30 kg ha ). Economical analysis showed that maximum value cost -1 ratio (4.1:1) was determined with incorporation of 5 t ha wheat straw plus -1 60 kg N ha .

...

 Syed Ishtiaq Hyder, Muhammad Arshadullah, Arshad Ali and Imdad Ali Mahmood*

EFFECT OF BORON NUTRITION ON PADDY YIELD UNDER SALINESODIC SOILS
... grain yield. Economical analysis showed that maximum value cost ratio was -1 (12.5:1) with the application of 1.5 kg B ha .

...

 Wabekwa, J.W., I.A. Sodagni and F.K. Mohammad*

PHYSIOLOGICAL GROWTH AND YIELD EVALUATION IN PMINERALIZED SUNFLOWER (HELIANTHUS ANNUS L.) UNDER SUDANOSAHELIAN AGRO-ECOLOGY, NIGERIA
...in 2011 and the combined analysis. Days to 50% flowering and head dry weight were not significantly influenced by P O rates and 1000- 2 5 -1 grain weights indicated that increased P O application (40-80 kg ha ) 2 5 enhanced grain filling efficiency and this influenced weight per 1000 -1 grains during both years. The application of 40 kg P O ha increased grain 2 5 -1 yield statistically in 2010 rainy season (2289.10 kg ha ) and the combined -1 -1

 Waqar Akhtar*, Nadeem Akmal*, Hassnain Shah*, Muhammad Azam Niazi** and Ayesha Tahir*

EXPORT COMPETITIVENESS OF PAKISTANI HORTICULTURAL PRODUCTS
...ge over the period under analysis and indicates a transition from comparative and competitive disadvantage to comparative and competitive advantage during the period under analysis. Tangerines, mandarins, clem have maintained relatively higher revealed comparative advantage as compared to other categories for the whole period under analysis. Onion export has revealed comparative advantage ...

 Naheed Zahra*, Muhammad Zubair Anwar*, Sonila Hassan** and Irfan Mehmood**

INSTITUTIONAL CREDIT ARRANGEMENT AND THEIR IMPLICATION ON AGRICULTURAL INCOME IN THE SELECTED VILLAGES OF RAWALPINDI DISTRICT
...dom sampling. Regression analysis was done to obtain the results. Ordinary Least Square (OLS) method was applied to generate the results. Results of this study revealed that there exists a positive and statistically significant relationship between institutional credit and agricultural income of the farmers. The other variables such as source of credit, ownership of farming machinery, access to market and family labour also have a positive and statistically si...

 Nasia Batool*, Imtiaz Ahmad Qamar**, Imdad Hussain Mirza** and Muhammad Fateh Ullah Khan**

MIXING LESS PALATABLE GRASSES WITH UREA, MOLASSES AND EFFECTIVE MICROORGANISMS AND ITS EFFECT ON CHEMICAL COMPOSITION AND DIGESTIBILITY IN GOATS
...ses + 4% urea. Proximate analysis of samples was carried out. Crude protein content of mixtures improved as compared with sole grasses. Digestibility of HC supplemented with urea, molasses and EM in various combinations was also studied in growing goats. The highest digestibility of DM in goats was recorded in HC + 4% urea + 4% molasses treatment (85.51%) followed by HC + 4% urea (78.57%) and HC + 4% urea + 4% molasses + 1:100 EM (78.00%).

...

 Khalid Naseem*, Naseem Bibi**, Saeeda Raza*, Amer Mumtaz*, Nouman Rashid Siddiqui*, Amina Bibi*** and Muhammad Ahsan Khan****

DEVELOPMENT, CHARACTERIZATION AND EVALUATION OF HIGH ENERGY BISCUITS FOR COMBATING MALNOURISHMENT AMONG CHILDREN IN PAKISTAN
...ntation level. Proximate analysis shows that T and T get the highest values for protein, zinc and iron. Results of all treatments were in acceptable range regarding sensory evaluation. These results indicate that biscuits can be successfully supplemented with chickpea and oat. According to sensory evaluation, biscuits containing 20% chickpea and 15% oat were found to be the best among all treatments and could be a potential composition for preparing high energ...

 Md. Nazirul Islam*, A. K. Azad**, Leon O. Namuco***, Teresita H. Borromeo***, Maria Lourdes O. Cedo*** and Edna A. Aguilar***

MORPHOMETRIC CHARACTERIZATION AND DIVERSITY ANALYSIS OF A MAKAPUNO COCONUT POPULATION IN U.P. LOS BANOS
... The principal component analysis revealed that fruit, nut and husk weight accounted for 99 % of the observed variation. Twelve morphological variables, grouped the population into 6 clusters. Common characters of palm belonging to cluster I are lower weights of fruit, nut and husk with shorter fruit equatorial diameter and shorter internodes. Palms belonging to cluster II have higher weight of nut and longer equatorial diameter. Palms of cluster III are chara...

 Tabinda Qaiser*, Ayesha Tahir*, Sajida Taj* and Murad Ali*

BENEFIT-COST ANALYSIS OF APICULTURE ENTERPRISE: A CASE STUDY IN DISTRICT CHAKWAL, PAKISTAN
...o calculate benefit-cost analysis of beekeeping in Chakwal. The data was collected from 25 beekeepers of Chakwal district because this enterprise is working more in areas like Chakwal, Sargodha, FatehJang and Mardan. Beekeepers were selected randomly for case study and they were interviewed for getting the necessary data to determine the costs and return of beekeeping and assess the scope of beekeeping. The benefit cost ratio of beekeeping was 1.4709 which sho...

 Muhammad Tayyab Mudassar*, Ehsan-ul-Haq*, Muhammad Naeemullah**, Muhammad Shakeel*, Muhammad Ashfaque*, Asmat un Nisa* and Javed Khan*

ECONOMIC REARING OF CHRYSOPERLA CARNEA (STEPHENS) (NEUROPTERA: CHRYSOPIDAE) IN INSECTARY
...nopsis.

...

 Khawer Jawad Ahmad*, Asif Razzaq*, Khalida Hamid Abbasi*, Muhammad Shafiq*, Muhammad Saleem** and Muhammad Arshadullah***

THYMOL AS CONTROL AGENT OF MITES (VARROA DESTRUCTOR) ON HONEYBEES (APIS MELLIFERA)

 Abid Hussain*, Khalid Mahmood Aujla* and Sonila Hassan**

ECONOMICS OF COW MILK PRODUCTION IN SINDH AND AZAD JAMMU & KASHMIR
...ermined and cost-benefit analysis of milk production by ecological zones was also conducted. Wide variations in share of feed sources in total feeding cost per annum, milk productivity and profitability of main cow breeds across selected areas have been observed. Concentrate feeding is the main cost item for cow milk production in irrigated areas of Sindh and constitutes more than half (54 %) of the total feeding cost per annum. While, in coastal and desert ar...

 Naveed Jehan*, Khalid Mahmood Aujla**, Muhammad Shahzad*, Abid Hussain**, Muhammad Zahoor*, Majid Khan* and Ahmed Bilal*

USE OF MOBILE PHONES BY FARMING COMMUNITY AND ITS IMPACT ON VEGETABLE PRODUCTIVITY
...le averages, correlation analysis and paired t-test. Average yield of bitter -1 gourd crop was 23569 kg acre with cell phone use of 8h per season. It is perceived that low communication cost and availability of information about wholesale market prices through SMS service and low cost calling packages will help farmers and market agents in improving bitter gourd production and its marketing, respectively.

...

 Ayesha Tahir*

FORECASTING CITRUS EXPORTS IN PAKISTAN
...anned to check the trend analysis of export quantity and export value for citrus in Pakistan. The findings of the study are based on citrus export quantity and export value data from 1990 to 2011. Two models of trend analysis were applied but most appropriate model for trend analysis of the present study was quadratic trend model. Forecasting was done from 2012 to 2016.The forecasted expor...

 Saima Rani*, Hassnain Shah*, Umar Farooq** and Bushra Rehman**

SUPPLY, DEMAND, AND POLICY ENVIRONMENT FOR PULSES IN PAKISTAN
...d and production. Demand analysis revealed that own price elasticity of demand was inelastic and less than one for both pulses. This implied the supply and demand gap of pulses in the country. Thus decline in pulses consumption could be attributed to disproportionate increase in pulses price that would affect the poor segment of population in fulfilling the protein demand. Hence there is need to increase production through improving management practices and di...

 Abid Hussain*, Khalid Mahmood Aujla* and Nouman Badar*

YIELD GAP DETERMINANTS FOR WHEAT PRODUCTION IN MAJOR IRRIGATED CROPPING ZONES OF PUNJAB, PAKISTAN
... least square regression analysis of wheat production by assuming Cobb-Douglas specification reveals that the number of irrigations, usage of farm yard manure and fertilizers contribute positively and significantly to wheat crop production. Coefficients of dummy variables for cropping zones indicate that farmers in the mixed cropping zone are obtaining better yield of the wheat crop as compared to their counterparts in other selected cropping zones. These resu...

 Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****

GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY
...nts (PC) and correlation analysis were employed to identify the suitable genotypes that can be further exploited in breeding programme. Significant associations were found between yield contributing trait, boll weight and fiber related trait, staple length. Earliness related traits, like st st days taken to 1 square and days taken to 1 flower had positive correlation with each other and both these traits also showed their positive association with ginning out ...

 Samiullah*, Mehmood Shah*, Kalim Ullah**, Rehmat Ullah*** and Ibrarullah****

PROFITABILITY OF WHEAT PRODUCTION IN DERA ISMAIL KHAN
...ability was applied. The analysis of data revealed that the average cost per acre was Rs. 24716.32. Similarly average production of wheat (output) was estimated to -1 -1 be 1618.40 kg acre . Thus the gross return was Rs. 45315.39 acre , whereas the net return (profit) from wheat production was Rs. 20599.07 -1 acre . It is thus concluded that profit is under the direct positive influence of price and output of wheat grain whereas cost had negatively affected th...

 Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari* 

ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...LD-5. Consequently, RAPD analysis can be used as a marker technique to ascertain the hybrid nature of offsprings and to evaluate parental lines in terms of genetic diversity.

...

 Nosheen Zahra*, Ghulam Sarwar* and Sher Muhammad**

COMPARISON OF GYPSUM AND POTASSIUM SILICATE FOR RECLAMATION OF SALINE SODIC SOIL
...essed through laboratory analysis. After this, rice seedling (Shaheen Basmati) was transplanted in all the pots i.e., 3 plants per pot. Necessary N, P and K fertilizers were applied at the recommended rate. After crop harvest, soil samples were taken for analysis of pH, electrical conductivity (EC), sodium adsorption ratio (SAR) and exchangeable sodium percentage (ESP) of soil. All the amendments when used alone or in combin...

 Muhammad Aamir Khan*, Abdul Saboor*, Rauf-i-Azam**, Abdul Qayyum Mohsin* and Fayyaz-ul-Hassan***

IMPACT OF AGRICULTURAL TRADE LIBERALIZATION ON INCOME INEQUALITY IN PAKISTAN

 Muhammad Shahzad*, Ayesha Tahir**, Naveed Jehan*** and Muhammad Luqman*

IMPACT OF DIFFERENT PACKAGING TECHNOLOGIES ON POSTHARVEST LOSSES OF STONE FRUITS IN SWAT PAKISTAN
...hod. Multiple regression analysis was employed to find relation between post-harvest losses and different factors at three different stages. Findings of the study revealed the channel of cardboard box technology accounted for post-harvest losses of 10.49% while at farm level, losses were 2.90%, at wholesale level 1.45% and retail level the losses were 6.14%. On the other hand post-harvest losses were 14.24% in wooden box channel; in which 6.10% occurred at far...

 Sajid Ali* and Abdul Jabbar**

GROWTH AND VARIABILITY IN AREA PRODUCTION AND YIELD OF SELECTED FRUIT CROPS IN KHYBER PAKHTUNKHWA
...een used for variability analysis. The findings of the study revealed that growth in production of selected fruit remained positive with area under fruit as a major contributor during period-I. Period-II witnessed either low or negative growth in fruit production except peach. Variability in fruit production was higher in period-II as compared to period-I. Main contributor to fluctuations in fruit production during period-I was variability in area under fruit,...

 Khalid Mahmood Aujla* and Abid Hussain*

ECONOMICS OF MILK PRODUCTION OF MAJOR DAIRY BUFFALO BREEDS BY AGRO-ECOLOGICAL ZONES IN PAKISTAN
...milk prices. Econometric analysis of milk production in the country revealed that use of green fodder and concentrates contribute positively and significantly to milk production.

...

 Syed Asif Imran Shah*, Shah Jehan Khan**, Abdul Aziz Baloch*** ,
Obaid Ullah Sayal**, Kalim Ullah* andShujaat Ali**

COMBINING ABILITY ANALYSIS IN VARIOUS INBRED LINES OF MAIZE (ZEA MAYS L.)
...n, during 2012. Variance analysis exhibited highly significant (P≤0.01) variation among genotypes for days -1 -1 -1 to 50% silking, ear length, kernel rows ear , kernels row , kernels ear and grain yield. Mean square values due to general combining ability were -1 highly significant (P≤0.01) for all studied attributes except kernels ear and grain yield. Highly significant variations for specific combining ability of direct and reciprocal effects were obs...

 Khurram Nawaz Saddozai*, Umme Rubab* and Abass Ullah Jan* 

STOCHASTIC FRONTIER ANALYSIS OF MAIZE FARMERS IN AZAD JAMMU AND KASHMIR, PAKISTAN

 Khurram Nawaz Saddozai*, Muhammad Nasrullah* and Noor P. Khan** 

STOCHASTIC FRONTIER PRODUCTION ANALYSIS OF TOBACCO GROWERS IN DISTRICT MARDAN, PAKISTAN
...stic production frontier analysis indicate that all variables were statistically significant and have portrayed positive contribution to tobacco production except fertilizer which was found significant but has revealed inverse relation with tobacco production. The mean technical efficiency was estimated at 0.85 depicting that tobacco growers can further amplify efficiency by 15% with given level of inputs. The inefficiency model estimates demonstrate that only...

 Umar Ijaz Ahmed*, Liu Ying**, Muhammad Khalid Bashir***,
Muhammad Amjed Iqbal*, Muhammad Rizwan*, Muhammad Mazhar
Iqbal****, Muhammad Rafi Qamar***** and Aftab Nazeer******

SPATIAL PRICE TRANSMISSION IN PAKISTAN: THE CASE OF WHEAT AND RICE MARKETS
...nthly retail prices. For analysis Vector Error Correction Model and Co-integration approach were employed. Results showed that wheat and rice market prices are integrated. Some markets have positive or negative transmission shocks on each other due to the different geographical locations and transport infrastructure. All the wheat markets are adjusted to price changes in the long run equilibrium except few markets. Unidirectional and bidirectional causality ex...

 Zafar Abbas*, Ijaz Ahmad**, Adnan Shakeel**, Muhammad Abdullah***, Muhammad Islam**, Sadiq Muhammad*, Ghulam Murtaza* and Mushtaq Ahmad**

EFFECT OF PHOSPHORUS FERTILIZER AND WATER STRESS ON PROTEIN AND PHENOLIC CONTENTS IN COTTON (GOSSYPIUM HIRSUTUM L.)
...titative and qualitative analysis of total protein and phenolic compounds, respectively. Proteins were greatly affected by fertilizer treatment and water stress, but phenolic compounds remained unchanged upon fertilizer treatment. However, they were greatly affected by irrigation and water -1 stress. Crop treated with 100 kg ha P O under water stress maintained high 2 5 protein content as compared to unfertilized and no water stress treatments. However, phenol...

 Imdad Ali Mahmood*, Arshad Ali**, Armghan Shahzad**, Muhammad Asif Masud Ghumman***, Muhammad Arshad Ullah**, Tariq Sultan** and Badar-u-Zaman**

CROP RESIDUES AND PHOSPHORUS EFFECT ON YIELD AND ECONOMICS OF DIRECT SEEDED RICE AND WHEAT GROWN UNDER SALINE-SODIC SOIL
...rop harvesting. Economic analysis of both the crops was carried out to see the best treatment with sufficient economic benefits as compared to without crop residues incorporation. Maximum net benefit of Rs. 92754 for direct seeded rice -1 and Rs. 69558 for wheat grown with 80 kg P O ha application under crop 2 5 residues incorporation was determined. Among P application treatments under no crop residues incorporation, the maximum net benefit (Rs. 75874 and Rs....

 Hafiz Saqib Habib*, Arshad Mahmood Malik*, Abid Ali** and Muhammad Aamir Khan***

SOCIOECONOMIC DETERMINANTS OF RURAL HOUSEHOLD FOOD EXPENDITURES IN RAWALPINDI
...expenditures. Regression analysis showed that 23 % of the changes in the food expenditures were due to explanatory variables. Household incomes, marital status of household head and number of earners variables have significant impact on households' food expenditures. The qualification of household head, age and gender were insignificant regarding impacts on households' food expenditures. All the socioeconomic factors were positively elastic regarding food expe...

 Muhammad Qasim*, Khuda Bakhsh**, Sultan Ali Tariq*, Mahmood Nasir***, Rashed Saeed**** and Muhammad Ather Mahmood****

FACTORS AFFECTING GROUNDNUT YIELD IN POTHWAR REGION OF PUNJAB, PAKISTAN
...g with its profitability analysis. The sample farms were classified into small, medium and large farms. Farm-level crop data were gathered during two cropping seasons i.e., rabi 2008-09 and kharif 2009. One hundred and forty groundnut producers were selected for collecting data using the well-structured questionnaire from two important districts recognised for area and production of groundnut. Results showed that large farmers allocated significantly higher ar...

Sumia Bint Zaman*, Waqas Farooq*, Sidra Majeed*, Hasnain Shah*, and Abdul Majid**

ASSESSMENT OF AGRICULTURE SERVICE PROVIDERS' TRAINING ON WATER CONSERVATION TECHNOLOGIES IN POTHWAR REGION
...he training. Descriptive analysis alongwith paired 't' test was used to measure the effectiveness of training. Results indicated higher satisfactory level as majority of the participants were strongly agreed (73%) and agreed (23%) that trainings were participatory and skill development oriented and as 96% agreed or strongly agreed that these were helpful in starting the business as assessment of agriculture service providers (ASPs). It can be concluded that th...

 Tasawar Sultana*, Farah Deeba* and S.M. Saqlan Naqvi*

HIGH-THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION OF MEDICAGO TRUNCATULA IN COMPARISON TO TWO EXPRESSION VECTORS
...gume plant for molecular analysis resulted in the development of efficient Agrobacterium-mediated transformation protocols. In current study, M. truncatula transformed plants expressing OsRGLP1 were obtained through GATEWAY technology using pGOsRGLP1 (pH7WG2.0::OsRGLP1). The transformation efficiency of this vector was compared with expression vector from pCAMBIA series over-expressing same gene (pCOsRGLP1). A lower number of explants generated hygromycin resi...

 Waqar Akhtar*, Muhammad Sharif**, Abdul Hayee Qureshi*, Khalid Mahmood Aujla*** and Muhammad Azeem Khan*

COMPETITIVENESS OF TOMATO PRODUCTION IN PUNJAB, PAKISTAN
... and Southern Punjab for analysis purpose under importable scenario by using import parity price. Results of PAM model revealed that tomato production in both regions of Punjab has competitiveness under prevailing market situation as indicated by positive private profitability and private cost ratio (PCR) which is less than 1. Competitiveness difference in two regions indicated that Central Punjab has more competitiveness at farm level in tomato production. Ec...

 Dilawar Khan*, Abid Saeed*, Junaid Ahmad*, Imtiaz Qamar**, Fazal Yazdan**, Saleem ud Din** and Muhammad Tariq**

ASSESSMENT OF RIPARIAN VEGETATION IN DHRABI WATERSHED AND CHAKWAL REGION IN PAKISTAN
...ivestock production. The analysis includes assessment and role of this riparian vegetation which is very helpful for integrated watershed management in different watershed areas. Dhrabi watershed is a catchments area of Dhrabi reservoir and lies in district Chakwal. There were 88 species reported in the study area. The five plant communities recognized in the Dhrabi watershed on the basis of importance value (IV) by using line transect sampling method. On the ...

 Mubbashira Nazir*, Saira Batool*, Sultan Ali Tariq**, Abid Hussain** and Ya Sakina**

INTERDECADAL GROWTH VARIABILITY AND DECOMPOSITION ANALYSIS OF FRUIT CROPS IN PUNJAB, PAKISTAN
...results of decomposition analysis indicated a relatively stronger stimulus of area expansion in production of citrus. While yield affect remained dominant in production of mango, guava and minor fruits across decades as well as during the study period. Chances of expansion in area of fruit crops are limited due to constraint of availability of agricultural land. Thus, to keep healthy growth in fruits' production, improvements in yield levels of fruits crop are...

 Hina Fatima** and Bushra Yasmin**

EFFICIENCY AND PRODUCTIVITY ANALYSIS OF PAKISTAN'S FARM SECTOR: A META-ANALYSIS*
...ations of Pakistan. Meta-analysis in present study provides the statistical efficiency analysis as well as statistical information regarding methodological, regional and crop specific influences on farm sector of Pakistan. The data rely on the published research papers on efficiency analysis of Pakistan's farm sector from 1971 to 2014. Three models separately estimated included methodologi...

 Ummad-ud-Din Umar*, Muhammad Aslam Khan**, *Ateeq-urRehman*, Abdul Hannan***, Syed Atif Hasan Naqvi*, Azhar Ali Khan****, Muhammad Asif Zulfiqar****

DEVELOPMENT AND VALIDATION OF POTATO LEAF ROLL VIRUS DISEASE PREDICTION MODEL BASED ON ENVIRONMENTAL FACTORS FOR FAISALABAD, PAKISTAN
...ble. Stepwise regression analysis was performed to select potentially useful predictor variables for model development. In stepwise regression analysis minimum temperature, relative humidity and rainfall emerged as the main contributing environmental variables for disease development. The model performance was evaluated based on coefficient of determination (R ) and the comparison of observed and predicted disease incidence....

 Ghazala Nawaz*, Muhammad Nawaz Malik**, Muhammad Hassan
Mushtaq***, Fraz Munir Ahmad****, Ali Abdullah Shah*****, Farooq
Iqbal******, Shinawar Waseem Ali*******, Zahida Fatima******** and
Amjad Khan*********

SURVEILLANCE OF BRUCELLOSIS IN LIVESTOCK IN RURAL COMMUNITIES OF PUNJAB, PAKISTAN
...ordingly, and chi square analysis of results, the true prevalence per 1000 animals in buffaloes was (7.86%), significantly (P<0.01) higher than small ruminants (5.84%) and cattle (2.05%). Being a subtropical region, geographically significant (P<0.01) variation was observed in burden and pattern of brucellosis i.e., the highest prevalence was in Central (14.81%) followed by South (0.5%) and northern (0.4%) Punjab. Considering the high mean prevalence and...

 

Shakir Ullah*, Aish Muhammad*, Iqbal Hussian*, Hafeez-Ur-Rahman**, Muhammad Zeeshan Hyder***, Muhammad Din**** and Nizamud Din

MORPHOLOGICAL VARIATIONS IN APRICOT CULTIVARS GROWN IN GILGIT BALTISTAN PAKISTAN

 Ehsanullah*, Muhammad Amjad Shahzad*, Shakeel Ahmad Anjum*, Ali Zohaib*, Muhammad Ishfaq* and Ejaz Ahmad Warraich*

EFFECT OF DIFFERENT SOWING METHODS AND PLANTING DENSITIES ON GROWTH, YIELD, FIBER QUALITY AND ECONOMIC EFFICACY OF COTTON
...sowing methods. Economic analysis showed that economic returns and benefit cost ratio (BCR) (1.52) was elevated by flat sowing on 25 cm apart rows. In conclusion, maximum seed cotton yield and economic returns can be acquired by flat sowing with 25 cm apart rows, while, fiber quality is independent of sowing methods.

...

 Syed Haider Abbas*, Ayesha Tahir**, Muhammad Asim***, Muhammad Sohail* and Riaz-ud-Din*

PRODUCTIVITY AND PROFITABILITY OF WHEAT PAK-13 AT DIFFERENT SEEDING DENSITIES UNDER RAINFED CONDITIONS
... and SD6: 175 kg/ha) The analysis revealed that the highest chlorophyll contents (49.5), number of tillers/m (240), biological yield (7125 kg/ha), and grain yield (2660 kg/ha) were achieved at seeding density 100 kg/ha and was significantly different among various levels of seeding density. Again, the profitability analysis show the maximum gross margin (Rs. 98920/ha) and BCR (3.44) at 100 kg/ha seeding density level.

...

 Nasarullah*, Raiz uddin*, Fakhar Uddin* and Muhammad Jamal*

INTERPRETING WHEAT GENOTYPES FOR GRAIN YIELD UNDER DIFFERENT REGIMES OF KHYBER PAKHTUNKHWA
...lly grown cultivars. The analysis of variance revealed significant differences among genotypes, locations and genotype by environment interactions for days to heading, days to maturity, plant height (cm), spike length (cm), spikelets spike , 1000-grain weight and grain yield. Flag leaf area (cm displayed non-significant differences across environments and its interaction with genotypes. Mean values across the environments range between 147.17 to 174.00, 190.83...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...chicken eggs. Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis reveale...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...nt and phylogenetic tree analysis indicated that theVps26A protein of sika deer was highly similar to that of Bos taurus. Expression analysis by real-time quantitative RT-PCR revealed that Vps26A gene had a higher expression level at day 90 than those obtained at day 60 and 30.
...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2 and Zafarullah Baloch3 

... Results from regression analysis revealed that age, education and experience are strong predictors in the model to increase the knowledge level of the respondents for production, processing and marketing of the dates in district Panjgur.
 

...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1Nisar Ahmad3, Ayesha Hassan1, Muhammad Kashif Prince1, Muhammad Luqman Sohail4,*, Mian Saeed Sarwar2, Hazrat Ali2, Tahir Usman2, Asadullah Khan2, Siffat Ullah Khan3 and Shahid Zaman5
David Beckwith Greene
...odes of continuation. An analysis of modes of continuation in Haydn’s “The Creation” shows that the continuity within the humankind aria and between it and the preceding arias models what Nagel is seeking: Both a fresh grasp on the discontinuity between humankind and other moments of natural history and a new kind of continuity.
...

Jesse M. Smith

... of ethnographic content analysis consistent with the inductive logic of a constructionist grounded theory. Combing elements of rhetorical theory with a sociological view of religious movement and marketplace, I identify the major themes and evolving narrative of this American religion in relation to secular historical and cultural conditions. The data suggest the rhetorical strategies revealed in Mormon leaders’ discourse on the secular is a part of the...
Yanxiao Meng1, Guihua Wang1, Dongmei Xiong1,*, Haixia Liu1, Xiaolin Liu1, Lixin Wang1 and Jianlu Zhang2
...f geometric morphometric analysis indicated significantly morphological variation in body shape among three morphotypes based on 18 landmarks. Principal component analysis (PCA) showed that the cumulative contribution rate of the first five principal components were 72.99%. CV1 (65.77%) and CV2 (34.23%) were well explained 100% of the observed variation among three morphotypes by Canonical variate analyses (CVA). The morphol...
Asmatullah1, Chaman Ara1,*, Shagufta Andleeb2, Maha Tahir1, Beenish Zahid1 and Madeeha Arshad1
...cteristics. Morphometric analysis of the cadmium treated groups indicated a significant (p<0.05 to p<0.001) reduction in fetal body weight, crown rump length, head circumference, eye circumference, forelimb and hind limb lengths and tail size compared to control fetuses. Morphological studies of fetuses showed abnormalities like micromelia, skeletal defects, hemorrhages, displacement of limbs, open eyelids and distorted axis. Histological defects include...
 Nazia Qamar1, Sher Khan Panhwar1,* and Ralf Riedel2,*
...2015 for stomach content analysis. Analysis of prey composition was done using permutational analysis of variance (permanova), with species, life stage (juvenile and adults), gender, and weather (rainy and dry season) as factors. Patterns of empty stomachs were investigated to estimate feeding intensity. Feeding intensity was estimated with logistic regression, using the same independent v...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
...on enzyme and sequencing analysis. The in vitro expression of the truncated protein was analyzed in E. coli with a molecular weight of 38kDa determined through SDS-PAGE and confirmed by Western blotting. The recombinant truncated protein was then purified from the culture media. The immunogenicity of the protein was studied in an animal experiment on mice, in which mice were injected subcutaneously. These findings suggest that the truncated...
Aqsa Gulzar1, Tariq Mahmud1,*, Rubina Munir2 and Asma Anjum3
...ectrometry and elemental analysis. The results were in great consistency with the structures of the synthesized compounds and confirmed the formation of the targeted compounds. It was found that the compound 4a was potentially anti-inflammatory active while the compound 4b was found more active anti-cancerous agent as compared to 4a.
...
Ewa Czerniawska-Piątkowska1,*, Magdalena Skibicka1, Barbara Cioch-Szklarz1, Jolanta Karakulska2, Karol Fijałkowski2 and Sonia Hiller3
...onal rearing, results of analysis of the swabs from the nasal cavities revealed the presence of pathogenic or opportunistic bacteria at the level of the average number of micro-organisms (11-50 CFU) or high (> 50 CFU), which negatively affected the health of the calves. However, in cold rearing there was recorded absence or presence of microorganisms in the nasal cavity in a small amount (<10 CFU).
...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
... and sequenced. Sequence analysis revealed single nucleotide polymorphism (T>A at position 483) and a sheterozygous condition (T>W at position 483). Generalized linear model was used to minimize the environmental effects. The variation i.e. “W” type heterozygous condition showed a higher average daily weight gain. Sequence analysis of LEP gene confirmed the presence of genetic variability among Lohi ...
Muhammad Umair Sattar1, Aysha Sameen1,*, Nuzhat Huma1 and Muhammad Shahid2
...oduced were subjected to analysis for physicochemical parameters, functional attributes, texture (hardness), appearance (L* value) and proteolysis by RP-HPLC. Use of guar gum as fat mimetic and cheese milk homogenization significantly (p<0.01) affected the composition, functionality, texture and appearance of LFMCs. LFMCs functionality and texture also improved during ripening but appearance was negatively affected. Reverse phase HPLC results indicat...

Amjad Ali1, Rajan Shrestha2, Ghaffar Ali1, Irfan Ullah1 and Salman Khan1 

... Resource use efficiency analysis shows that marginal value product (MVP) to marginal factor cost (MFC) ratios for labor days, irrigation, urea, FYM and pesticides are more than unity indicate underutilization of these resources which underlines scope of expansion in these resources. Study results illustrate that land, seed quantity, tractor hours and DAP are over utilized which need readjustment. Study recommends improvement in extension services to guide far...

Amjad Ali1, Rajan Shrestha2, Ghaffar Ali1, Irfan Ullah1 and Salman Khan1 

... Resource use efficiency analysis shows that marginal value product (MVP) to marginal factor cost (MFC) ratios for labor days, irrigation, urea, FYM and pesticides are more than unity indicate underutilization of these resources which underlines scope of expansion in these resources. Study results illustrate that land, seed quantity, tractor hours and DAP are over utilized which need readjustment. Study recommends improvement in extension services to guide far...

Ejaz Ashraf1*, Hafiz Khuram Sharjeel1, Raheel Babar2, Muhammad Junaid1, Qamar Iqbal3, Rabia Rasheed1 and Nosheen Fatima1 

...nse rate was 21.94%. The analysis showed that training and visit program and demonstration methods were dominant approaches used in technology transfer. The mean of the results showed that low allocation of budget, massive operational area to give satisfactory coverage, less use of multimedia, unavailability of training facilities to EFS, manifold duties assigned to extension officers and top-down dimension of planning of implementation of extension approaches...
Sana Zahra
...onfirmed through linkage analysis using Short Tandem Repeat Markers (D7S2420, D7S2459 and D7S2456). p.Y556C was identified as causative mutation by Sanger Sequencing. A known missense mutation p.Y556C in exon 15 of SLC26A4 gene was identified in all the four families. It may be concluded that p.Y556C mutation is a recurrent mutation in Punjab province of Pakistan.
...
Mubashar Hussain, Muhammad Faheem Malik, Sehreen Siddique, Muhammad Umar, Tamkeen Zainab, Fatima Zafar
Humaira Jabeen and Nazia Jamil*
...ared spectroscopy (FTIR) analysis of the extracted bio-plastic indicated the presence of the C=O functional group specific for the presence of polyhydroxyalkanoates, the absorbance peaks were specifically at 1600 cm-1. Ribo-typing of all the six strains were carried out, strains identified as Exiguobacterium indicum, Bacillus acidiceler, Acinetobacter seohaensis, Serratia proteamaculans, Trabulsiella guamensis and Serratia grimesii and...
Muhammad Tariq Zahid1, Farah Rauf Shakoori2, Soumble Zulfiqar3, Khalid A. Al-Ghanim4 and Abdul Rauf Shakoori3,4*
...the medium. Quantitative analysis showed that TfCuMT is a copper inducible gene, the basal transcriptional level of which increased within 15 min of 10 µg/ml copper exposure upto 87.3 folds that later on decreased. Accordingly, maximum uptake rate was observed between 15 to 30 min of copper exposure indicating possible role of TfCuMT as copper metallothionein. T. farahensis and TfCuMT can be used as biotechnological tools for ...
Ehtisham Shakeel Khokhar*1, Amir Shakeel1, Muhammad Amir Maqbool1, Muhammad Khubaib Abuzar2, Sumaira Zareen2, Syeda Sana Aamir4 and Muhammad Asadullah4
...ng ability and heterosis analysis was done to identify the potential parents and best performing hybrids for seed cotton yield, yield contributing traits and fiber quality parameters. Six lines, viz., VH-259, IUB-222, CRS-456, AA-703, KZ-191, PB-39 and four testers PB-39, CIM-608, BH-163 and PB-900 were crossed in line × tester crossing fashion to develop 20 F1 hybrids. Analysis of variance displayed signifi...
Shagufta Andleeb*1, Shabana Shaukat1, Chaman Ara2
...logical and morphometric analysis. Liver tissues were dissected out from each group for histological analysis. Cadmium chloride induced significant developmental abnormalities such as reduced body weight and crown rump length, exencephaly, ablepharia, crossed beak, gastroschisis, crooked toes, non-alcoholic fatty liver and pyknosis. Fresh garlic juice has successfully attenuated these toxicities and increased the body weight...
Riyadh S. Aljumaah*, Mohammed A. Alshaikh, Abdulrahman Jarelnabi, Mutassim M. Abdelrahman and Mansour F. Hussein
...ies. Logistic regression analysis and estimation of odds ratio revealed significant association of the sex and location of the camels with N. caninum seropositivity. On the other hand, neither the breed nor age of the camels was significantly associated with seropositivity.Studies are needed to evaluate other potential risk factors and to assess the pathogenic effects and economic consequences of neosporosis in camels.
...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...Furthermore, correlation analysis showed a positive relationship between the mRNA expression of TLR4 and concentrations of TNF-α and IL-12. These results revealed that although there were no significant effects of LPS injection on TLR4 mRNA expression in lymphoid organs, the response to LPS may not mainly rely on the transcription of TLR4, and the downstream molecular response in the immune system may contribute more to the TLR4 sign...
Abdul Majid Ujjal2, Sharmin Akter1* and Suman Paul1 
...g winter season. Similar analysis was also done for HS and like FMD, highest prevalence (16.14%) found in Jaintapur upazila. HS prevalence also found significantly higher in young buffalos (18.56%). HS prevalence was significantly higher in male (12.73%) than female. Epidemic curves of HS showed a clear year round occurrence with peaks in summer season. Though, estimates could not expressed real disease burden because of using information of hospital register,...
Ligia Neves Scuarcialupi, Laila Andreia Rodrigues Beserra, Julia Rosas Hochheim, Rodrigo Martins Soares, Fabio Gregori*
...analyzed by phylogenetic analysis. There were 5 RVF positive samples (5.15%) in 2 broilers, 1 laying hen and 2 breeders. Only one RVG sample (1.03%) was detected in laying hen, in coinfection with RVF. This data contributes to a better understanding of rotavirus distribution in Brazil.
...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

...ent virus clusters. This analysis revealed a high evolution rate for clade 2.2.1.2. Additionally, analysis of selection pressures in the HA gene revealed a positive selection pressure. Deduced amino acid analysis revealed characteristic mutations at HA antigenic sites besides two other mutations (129∆, I151T) that were found to be stable in recent subclade 2.2.1.1 isolates from human...
Waqas Ali1,*, Arshad Javid1, Syed Mohsin Bhukhari1, Ali Hussain1, Syed Makhdoom Hussain2, Hira Rafique1
...suitable for statistical analysis and provide inappropriate picture regarding distribution, abundance and status of target species. Current review inspects different sampling techniques, various issues related to surveys design, application and ethical issues connected with these sampling techniques. It can be concluded from the literature survey that all sampling techniques have biasness related to geographical position, habitat and species under consideratio...

Muhammad Iftikharul Husnain1, Aneel Salman1, Inayatullah Jan2*, Tahir Mahmood3 

...arrying out an empirical analysis on the temperature-agriculture nexus to obtain reliable estimates of the regression coefficients. Policymakers should pay more attention to combat negative impacts of rising temperature. This will have an impact on the agriculture and hence on the livelihood of the mass population residing in South Asian countries. 

...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
... groups. Gene expression analysis revealed a significant increase in BAX expression in groups supplemented with SFF 20% and 40%, whereas the expression of BCL-2 was significantly increased only in the 40% SFF supplemented group. The ratio of Bax to Bcl-2 was significantly increased in the embryos derived from oocytes matured in 20% and 40% SFF supplemented media. HSPB1 expression was significantly increased in group matured in 20% SFF. The relative expression ...

Nadia Faqir1, Aish Muhammad2*, Ghulam Muhammad Ali2, Armghan Shehzad2, Hafeez Ur Rahman3, Farhatullah4 and Muhammad Zeeshan Hyder

... date fruit and sequence analysis of two genes (maturase k and geranyl geranyl biphosphate reductase) from chloroplast region. Analysis of variance has revealed significant variation among the studied date palm samples pertaining to the studied physical traits and chemical composition of fruit. Sequences of the maturase k gene has complete identity to the reference genome of ‘Khalas’ cultivar. While geranyl geran...

Murad Khan1* and Muhammad Afzal2 

...ltiple regression model, analysis of variance (ANOVA) and independent t test were used. The average profit of large size farm was Rs. 85228 followed by medium and small farm that were Rs. 58049 and Rs. 36090 respectively. The results of independent sample t-test indicated that there is no significant difference between the profit of the sole proprietors and partnerships. Further results of analysis of variance (ANOVA) reveal...
Leila A. Kaimbayeva1,*, Elena S. Malysheva2, Rashit Kazikhanov3 and Saule R. Kazikhanova4
... The results of chemical analysis revealed the nature of protein substances, altered рН value of the meat and the intensity of glycolysis. All these parameters were indicative of autolysis process in the meat. The levels of water-binding capacity and structural-mechanical properties of red deer meat that occurred during the course of autolysis were further confirmed by the histological changes in the meat. Specifically, it was observed that post-mo...

Abdur Rehman1*, Wang Jian2, Noor Khan3 and Raheel Saqib

...et risk. The market risk analysis of major crops such as coarse grain rice, wheat, corn, cotton and soybean are determined using the VaR (Value at Risk) model. The practical consequences indicated that the normal distribution model is not appropriate used in evaluating the major crops market risk due to various degrees of market risk in various crops such as the cotton and soybean crops with higher levels of market risk, while the coarse grain rice with the me...

Zakirullah Jan1, Shamsher Ali1*, Tariq Sultan2, Muhammad Jamal Khan1, Zahir Shah1 and Farmanullah Khan1 

... shoots and roots tissue analysis for various nutrients uptake/concentration. The data revealed that all strains of cyanobacteria had significantly (p < 0.05) improved the nutrients up-take/concentration and agronomic traits of rice crop as compared to control. Among the strains, the Gloeobacter strain (MMF-4) surpassed by augmenting all agronomic parameters of rice crop like plant height (85 cm), plant fresh weight (45 g), dry weight (24.5 g) and root dry ...

Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1 

...tes of T. harzianum. The analysis of the bi-variate data, using Unweighted Paired Groups Method Averages, divided the indigenous isolates of Trichoderma, into four well-defined clusters. These results clearly revealed that there were considerable genetic differences among the Trichoderma isolates. While, there was no correlation, found between RAPD clusters and aggressive groups (AGs). Isolates belonging to different AGs were found scattered among the four RAP...

Qasim Iqbal1, Safdar Ali1*, Muhammad Naveed Tahir1, Obaidullah Shafique1, Bashir Ahmed Khan2, Ijaz Ahmad3 and Ihsanullah Khan4  

...espectively. The Cluster analysis also determined SF 16003 as best hybrid which was at a very close distance from the group of SF16010 and SF 16002. 

...
Arshad Mahmood Malik1, Khalid Mahmood Mughal2, Sarfraz Ahmed Mian2 and Atta Ullah Khan2
...us factors identified in analysis have been further examined and measures have been suggested to improve supply of tomato in Pakistan. 

...

Saleem Ashraf1*, Ashiq Hussain Sangi1Zakaria Yousaf Hassan3 and Muhammad Luqman2

...is study was an overtime analysis of secondary data to calculate compound growth and forecast area, production and average yield in the Punjab Pakistan For this purpose, data collected from government reports for the period of 1990-2017 was criticaly examined. Empirical results indicated that area under cultivation showed negative compound growth rate while production and average yield showed positive compound growth in Pakistan. While, in Punjab, compound gro...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...confirmed by Immuno blot analysis using anti-interferon α-2 and anti-thymosin α-1 antibodies. Purified IFNα2-Tα1 demonstrated anti-proliferation activity as assessed by MTT assay. This study also showed that N-terminal fusion of SUMO withIFNα2-Tα1 is effective for its soluble expression and to make its purification process more convenient.
...

Ashraf Sayed Awaad1 and Mohamed Zaki Fathy2*  

...ss anatomy, CT and image analysis. The results showed wider lumen of the vertebral canal at L5- L6 and the space between the inner part of the vertebrae and the spinal cord is wider than those at the level of L6- S. Moreover, the space between the spinous processes in this area was 1.58 ±0.089 cm and easily to be detected. The needle was inserted properly and more easily in the epidural space of the selected new site by using US. In conclusion, The L5-L...

Samiullah*, Mohammad Aslam Khan and Atta-ur-Rahman  

... survey. For micro level analysis, five villages were randomly selected for detailed and intensive study. In addition to questionnaire survey, detailed focused group discussion was also arranged with the key stakeholders. The analysis reveals that rapid urban expansion has resulted in reduction of farmland and sub-division of landholdings into smaller land fragments. Beside urbanization, a major contributor to fragmentation ...
Nadia Saeed1,Mian Sayed Khan2, Habib Ahmad3, Muhammad Abdullah4 and Muzafar Shah5,*
...ique. After Quantitative analysis of both treated and untreated trees, it was observed that treated trees samples showed decline in nematode number as compared to untreated trees. Organic amendments effectively controlled the nematode population. Among all applied organic amendments castor oil cakes and cow dung were found more effective and saw dust was least effective. The studies showed that organic amendments were significantly effective against nematodes....

Shamsher Ali1*, Sarmad Khan1, Muhammad Jamal Khan2, Naveed ullah4, Muhammad Rashid3 and Wiqar Ahmad1 

...r emergence. Statistical analysis of the data showed that both soils and varieties significantly (p<0.05) affected per cent emergence and growth. Interactions between soils and varieties were significant (p <0.05) regarding emergence and plant heights. From the interactions it is clear that under normal soil conditions, emergence percentage of Pirsabak-2004 variety was maximum (100 %), under moderate soil salinity, emergence percentage of Siren variety w...
Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*
...Hela cells. Western-blot analysis displayed neurexophilin 1 in the cell and cell culture fluid of DF-1 cells and Hela cells, and higher expression of neurexophilin 1 in cell culture fluid of DF-1 cells than that of Hela cells. These findings suggested that DF-1 cells are effective and a better choice than Hela cells to do researches on the function of neurexophilin 1 gene.
...

Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair

...ignment and phylogenetic analysis. The eight studied consensus amino acid sequences of Meq proteins possess several amino acid mutations associated with the MDV virulence and a unique distortion in the Proline repeats (Proline-to-Alanine) at position 176 in the Egyptian MDV strains. The Phylogenetic analysis grouped the eight analysed sequences with the previously investigated Meq from Egypt (2011-2013) together with the ver...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...-PCR. We carry karyotype analysis out and find that chromosome is 2n=78 and shows normal. CAFSCs in mid period of subculture propagate fastest from cell cycle examination on Flow Cytometer and growth curve using CCK-8 kit on ELIASA. CAFSCs are induced to neuron and epithelium (epiblast), adipocyte (mesoblast), hepatocyte (entoblast).
...
Ali Zaman1,*, Abdul Haleem1, Sajjad Ur Rahman2, Adnan Amin3 Asma Saeed1, Sana Ullah4 and Naimat Ullah4
...2% (22/130). Statistical analysis confirmed a significant (P<0.05) difference of sero-prevalence between the districts. There was a significant (P<0.05) association of occurrence of the infection with the type of season, health status, vaccination, housing system, biosecurity and housing zones and non- significant (P>0.05) association with rearing system. To the best of our knowledge, this is the first sero-prevalence report of av...
Musrrat Fatima, Muhammad Saad Khan, Hamid Rashid, Asim Mehmood, Sumaira Kanwal, Muhammad Asif Rasheed and Farrukh Jamil*
Yakup Erdal Ertürk
...ARS) as a non-parametric analysis technique was preferred in the description of the influential factors and their interaction effects for each gender. Some probable factors such as age, province, education level, experience, social security, lands, and the reason at performing animal production were recorded on breeders. Also, first fattening weight and fattening period of the beef cattle were recorded. It was determined that predictive models based upon MARS ...
Zihong Chen1, Ling Xu2, Xiaona Yang2, Yaguan Zhang3* and Yuming Yang4
... multigene phylogenetics analysis involving 5.8S–ITS, nrSSU, nrLSU, EF–1α, RPB1 and RPB2. In multilocus phylogeny, M. baoshanense was grouped as a sister clade to Metarhizium majus, Metarhizium guizhouense, Metarhizium lepidiotae and Metarhizium indigoticum, but conclusively separated from these allied species with high support value. Morphological character differentiated M. baoshanense from its rela...
Nina Bao1,*, Yongguang Yin2 and Panpan Wang3
...C, respectively. IR analysis showed that the extraction has hydroxyl and amide groups, which was proved to be cerebroside. Meanwhile, bioactivity showed that the extraction has antibacterial activity, and the purified one is better than the crude one.
...
Bin Wang1, 2, Qianji Ning1,*, Qian Wang2, Wei Peng2, Tong Hao2,*and Jinsheng Sun2,*
...cused on the independent analysis of single protein, rather than a protein-protein interaction. In this work, with the systematic point of view, the subcellular location of 830 unidentified proteins were annotated based on the previously reconstructed protein-protein interaction network (PIN) of E. sinensis using the bioinformatics analysis method, which accounts for 91.9% of all un-localized proteins in the network. ...
Wael S. El-Tohamy1,*, Russell R. Hopcroft2 and Nagwa E.M. Abdel Aziz3
Selçuk Kaplan
...ater buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T1145G, T1151G and C1221T) and one monomorphic site (C1071T) in Anatolian water buffaloes. To the best of my knowledge, this is the first study that fully describes seven polymorphic sites of bubaline leptin gene in Anatolian water buffaloes.
...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
...es of growth. Microarray analysis of data at 12 vs 18 months, 12 vs 24 months, 12 vs 30 months, 18 vs 24 months, 18 vs 30 months and 24 vs 30 months uncovered a total of 10625 differentially expressed genes (P ≤ 0.05; no fold change). The dynamic impact approach (DIA) bioinformatics tool was used to uncover the most-affected biological pathways among the DEG. Ingenuity pathway analysis (IPA) software also was used to anal...
Nasreen Ishaq1, Arif Rasheed Malik2, Zameer Ahmad1, Saad Ehsan Ullah3
...ata, fingerprinting, DNA analysis, anthropometry, identification of sex, assessment of age, determination of height and blood groups identification. Among these, DNA analysis and dental data provide easiest identifications, however, these techniques are expensive and not readily available necessitating additional techniques for identification. One of such novel approach is cheiloscopy i.e. study of lip print patterns. <...

Ghulam Abbas1, Muhammad Jawad Asghar1, Muhammad Rizwan2*, Muhammad Akram1, Jaffar Hussain1 and Fiaz Ahmad1 

...ated traits. The genetic analysis of mungbean germplasm revealed high genotypic (GCV) and phenotypic (PCV) coefficients of variability for biological yield (GCV% = 31.70, PCV% = 33.58), harvest index (GCV% = 27.80, PCV% = 30.16) and seed yield (GCV% = 25.28, PCV% = 27.54). While heritability estimates were high for all the traits except days to maturity and clusters per plant. Biological yield, harvest index and seed yield depicted high estimates of heritabili...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
... outbreaks, the sequence analysis of hexon gene of 10 isolates associated with IBH and HPS was determined. Sequence alignment and phylogenetic analysis based on direct sequencing of hypervariable region of hexon gene grouped these sequences into two distinct groups, three FAdV isolates were clustered together belonging to fowl adenoviruses C species and serotype FAdV-4. The similar viruses have been commonly isolated since l...
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Haijun Cui1 and Xin Zhang2
... in group 4. Egg quality analysis demonstrated the significant increases in shell thickness among declawed treatments and yolk color in group 2. Overall feather score of layer breeders was not a significant difference, but the neck region had the lowest feather score in all groups. Preferential trimming methods were the removal of the first and third or second and fourth toes of day-old chicks in Hy-Line strain.
...
Muhammad Nusrullah*1, Muhammad Younus2 and Yasir Nasir3
.../b> | Arterial blood gas analysis is an important test for determining acid base balance of the body. Chronic obstructive pulmonary disease is characterized by chronic airflow limitation which is not fully reversible and it can lead to respiratory failure.
Objective: To determine the correlation between arterial and venous blood gases in patients presenting with chronic obstructive pulmonary disease.
Material and Methods: ...
S. Jalbani1, N. T. Narejo2,*, P. Khan2 and Y.M. Jalbani3
...
...
Ansar Ahmed Abbasi1,*, Kathrin Blasius2-4, Imtiaz Ahmed6, Hao Hu7, Sylvie Picker-Minh2-4,8, Muhammad Nasim Khan5, Khalid Hameed1, Aneela Gulnaz1, Zahid Latif5, Abdul Rauf5 and Angela M. Kaindl2-4,8
...encing and cosegregation analysis. We identified a previously described mutation c.4802C>G (p.S1601*) in the ASPM gene in both families. This study further underlines that mutations in the ASPM gene are a common cause for microcephaly in the Pakistani population and updates our knowledge regarding the frequent involvement of ASPM in microcephaly.
...

Jahangir khan* and Abbas Ullah Jan 

... and a linear regression analysis conducted to identify important determinants of households’ WTP. Results show that 93% of respondents are willing to pay a price premium of PKR. 14. Empirical findings reveal that socio-demographic characteristics like age, education and income have positive significant effects on households’ willingness to pay. Findings of the study also reveal a significant influence of employment status of the household’s ...

Kashif Akbar* and Muhammad Ayub 

... flour). Physicochemical analysis of the cookies showed the different trend from F0 to F6. Mean score for moisture content increased and the ranged between F0-F6 (3.24-3.29%), other physicochemical parameters showed decreasing trend like ash (0.80-0.73%), fat 24.23-23.94%), protein (8.20-7.68%), fiber (6.47-6.38%) while NFE increased slowly (57.86-58.60%). The color of the cookies ranged from F0-F6 (6.88-6.93), taste ranged F0-F6 (6.82-6.04) and texture ranged...

Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla

...spectively. Phylogenetic analysis revealed that Hail PPRV strains clustered in four separate groups within lineage IV indicating genetic divergence based on N gene. These findings further support the continuous characterization of PPRV strains in small ruminants to support the global eradication plan in 2030. 

...
Tanveer Hussain1,*, Masroor Ellahi Babar1, Marcos De Donato2, Abdul Wajid1, Asif Nadeem3, Zahoor Ahmad3, Waqas Ahmad Khan4, Sunday O. Peters5 and Ikhide G. Imumorin6
Wang-Dui Basang1, Tian-Wu An2, Luo-Bu Danjiu3, Yan-Bin Zhu1, Shi-Cheng He3, Xiao-Lin Luo2, Wei-Wei Ni4, Xiao Wang4, Shu-Zhu Cheng4, Jian Wang4 and Guang-Xin E4,*
...population. Phylogenetic analysis revealed that these four populations separated into two haplogroups; the first included SN, DX, and NR, and the second included JL. However, no significant divergence was found among the ecotype populations using a pair-wise difference comparison (FST). Thus, the Naqu yak has multiple maternal origins and demonstrates high diversity within 4 geographic ecotype yak populations. In addition, the yaks exhibit so...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
...used for further genetic analysis. RAPD makers (n=8) produced 245 bands (30.62±2.87 Mean±SE / primer) of different molecular weights (126-3342 bp), of which, 96 were population specific. Polymorphism was (37.71±5.29%; mean ± SE), with the highest in Muzaffarabad population (54.29%), followed by Poonch (43.67%) and Neelum (36.73%). Values of Shannon’s (I: 0.129-0.200) and Nei’s genetic diversity (He: 0.082-0.117) indices ...
Jingen Xu1, Yang Liu2, Erhui Jin1, Youfang Gu1, Qin Zhang3 and Shenghe Li1,*
...e subsets. Gene ontology analysis revealed that differential gene expression involved in biological processes, including the G-protein coupled receptor protein signaling pathway, intrinsic to membrane, cell junction and ion transport. KEGG pathway analysis showed that the neuroactive ligand-receptor interaction was significantly enriched. The results offer a foundation for exploration of immune functions and genetic control ...

Muhammad Khalid1,2 and Muhammad Naeem2* 

...ign: justify;">Proximate analysis implies percent composition of moisture, protein, lipids and carbohydrates. The purpose of this study was to affirm the proximate composition and nutritious status of Ctenopharyngodon idella. Standardized methods were used to determine the proximate composition of 72 samples. On wet weight basis, mean percentage for water was 80.76 %, ash 3.40 %, 4.31 % for fat and 11.53 % for protein in C. idella. Percent water contents showe...
Ai Guo1,2,3, Jiaguang Xiao4, Binbin Shan4, Tianxiang Gao5 and Yongdong Zhou3,*
...em repeats. Phylogenetic analysis was performed based on 13 concatenated mitochondrial protein-coding genes from 8 Coilia mitochondrial genomes. The results supported that C. lindmani at first clustered with C. nasus and C. grayii which had close relationships (d=0.028), then clustered with C. mystus which exhibited obvious genetic differentiation between C. mystus N and C. mystus S (d=0.083). C. reynaldi...

Shahen Shah1*, Manzoor Hussain1, Arshad Jalal1, Mohammad Sayyar Khan2, Tariq Shah1, Muhammad Ilyas1 and Muhammad Uzair3 

...t was concluded from the analysis of data that nitrogen at the rate of 150 kg ha-1 with sulfur at the rate of 45 kg ha-1 applied 50% at sowing + 50% at tillering significantly contributed towards the economics and reproductive traits of wheat. 

...

Sadiqa Begum1, Murad Khan2* and Noor Pao Khan

...economic and comparative analysis of wheat crop. Furthermore, two villages were selected from each district i.e. Lakarai and Barbar Opazai of district Peshawar and Khawo and Shamshad Abad of district Mardan. A questionnaire was used to collect data proportionately from 93 sample respondents. Budget allocation technique is used for calculation of cost, revenue and profit and dummy approach is used for comparative analysis whi...
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3,*
...c Gobiinae. Phylogenetic analysis also demonstrated clade Amblyopinae included all three species and that O. lacepedii has been proved to be a much closer affinity to Odontamblyopus sp. than O. rebecca. Our study theoretically provided a supplementary proof and significant information for future taxon studies.
...
Habib-ur-Rehman1*, Saima Mirza1*, Mansoor-ul-Hasan2, Qurban Ali3, Hafiz Abdullah Shakir4, Muhammad Yasir5
...ane separately. Periodic analysis for the repellent effects was carried by impregnating each filter paper (half-disc of filter papers) with micropipette at three concentrations (5, 10 and15%) of each of the plant extract. The repellence was recorded after 24, 48 and 72 h of the treatments application. The findings of experimental trials presented significant mean repellency 79.15% of T. castaneum (with methanolic extract of C. paradise)fol...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...ignment and phylogenetic analysis were performed to compare SlNPV- FSD15 based on Lef-8 with other Lef-8genes sequences clearly showed that our SlNPV-FSD15 isolate belongs to Spodoptera litura associated NPVs. The biological activities of this NPV isolates were investigated under laboratory condition. The highest mortality of S. litura was observed at early instars. Against second instars of S. litura, LC50 ...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...ng PCR while association analysis was used to test the likelihood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalosporin, lincosamide, tetracycline, macrolides and aminoglycosides. No resistance was observed against vancomycin and linezolid. Among genes involved in host immune evasion, Staphylococcus protein A (spa) was ...
Naeem Ahmad1,* and Zulqurnain2*
...ing, however an economic analysis for both strains determining benefit to cost ratio is pre-requisite before final recommendations.
...

Aftab Khan, Shahid Ali, Syed Attaullah Shah and Muhammad Fayaz 

...maize growers. Zone wise analysis of forecasting effects of temperature shows that increase in temperature by 1 °C in 2040-2050 and by 2 °C in 2060-2080 will significantly increase net revenue of maize growers in Northern and Eastern zones, but insignificantly in Central zone. An increase in temperature by 1 °C in 2040-2050 and by 2 °C in 2060-2080 will decrease net revenue in Southern zone. Government needs to encourage research institutes for...

Muhammad Faheem Jan1*, Asad Ali Khan1, Waqas Liaqat1, Haseeb Ahmad1, Muhammad Dawood Ahmadzai1 and Wazir Rehan

Nusrat Habib1*, Asim Zubair2 and Aisha Siddiqua3 

...hwa, multiple regression analysis was applied. The model pointed out that the area under sunflower have opposite relation with the seed cost, land rent, area under tobacco, area under sugarcane and price of sunflower seed, while positively related to the area under sunflower with the variables such as total operational holding, sunflower yield and sale price of sunflower. In case of determining yield of sunflower use of DAP and organic manure were significant ...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
... samples for biochemical analysis (blood glucose, cholesterol and total protein) and gills for histology were taken after 96 hrs. After acute exposure to pesticide, significant changes were observed in serum biochemistry and histology. Serum glucose and cholesterol were increased while total protein was decreased. Histopathological result reveled that gills of experimental fish was damage severely resulting Necrosis, Damaged nuclei , rupturing of epithelial ce...

Muhammad Abu Sufyan Ali, Syed Attaullah Shah*, Ghaffar Ali and Muhammad Fayaz 

...lts from Hedonic Pricing analysis show that location, environmental and agricultural characteristics of a farmland determine its price. Residential and commercial encroachment on fertile irrigated farmlands at urban fringe is alarming. More than half of the sampled transacted farmland parcels were used for residential and commercial units’ construction. Farmland potential for commercial or residential use creates a value expectation and it capitalizes in...

Qaisar Mehmood1*, Muhammad Riaz2, Maqbol Hussain Sail1 and Muhammad Moeen1 

...ear regression model and analysis of variance techniques are used which show that variety, sowing mode, nitrogen fertilizer and phosphorous fertilizer have significant effect on the wheat yield. Irrigation mode and weedisides spray also has much impact on the wheat yield. 

...
Qiang Li1,2, Guo Qiao2, Li Wang1, Jipeng Zhang1, Ruijun Li1, Ping Ni1, Yi Guo1 and Shigen Ye1,*
...h 16S rRNA gene sequence analysis and Vibrio spp.-specific PCR amplification, followed by pathogenicity determination. Results showed that the isolates belonged to 10 genera, including Vibrio (72%), Staphylococcus (9%), Pseudomonas (4%), Bacillus (4%), Vagococcus (3%), Shewanella (3%), Planococcus migula (4%), Exiguobacterium (1%), Enterobacter (1%) and Kocuria roseus (1%). Vibrio ...

Amar Razzaq1, Abdur Rehman4*, Abdul Hassan Qureshi2, Iqbal Javed3, Raheel Saqib5 and Muhammad Nadeem Iqbal6 

... to conduct the economic analysis of high efficiency irrigation systems in Punjab province of Pakistan. We also measured and compared the water productivity of modern and conventional-irrigated farms. We used primary data collected from 120 farmers located in Nurpur Thal, Bhalwal, Sargodha and Lodhran districts of Punjab province. These areas were purposively selected based on relatively higher concentration of HEI infrastructures installed on the farms. Sprin...

Sajad Ali1*, Syed Asghar Ali Shah2, Jaffar Ali3, Muhammad Nadeem Iqbal4, Arshadullah Jadoon5 and Farmanullah

...ating marketing marginal analysis and cross-tabulation were also been employed. The studied statistics of the land size of chosen pea’s farmers fluctuated amongst 0.878 to 31.26 acres having mean of 4.524 acres. Moreover, maximum merchants and Beoparis credited the output of peas from the farmers directly from the local market that is Mansehra and Haripur. It is worthwhile to mention that the public sale arrangement was not totally available in the local...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...onal level. Quantitative analysis through real time PCR demonstrated that the mRNA level increased enormously – up to 18.3 times - under copper induction. Time course study revealed a bimodal pattern of expression with two maxima, first at 15 min and second at 90 min exposure time. The role of CueO in copper detoxification as well as its laccase activity makes it suitable for biotechnological applications.
...
Asad Ullah1, Umar Sadique Khattak2, Sultan Ayaz1, Muhammad Subhan Qureshi2, Imad Khan1, Ibad Ullah Jan2, Irfan Khattak1, Raheela Taj3, Sahar Nigar4,    Naimat Ullah Khan1,Mumtaz Ali Khan5 and Muhammad Luqman Sohail6,*
...s was 5.88%. Statistical analysis revealed significant association of age (OR= 3.267; CI = 1.686-6.331) and herd size (OR = 2.600; CI = 1.421-4.760) with CCIT positivity. Similarly, induction of new animals into the herd (OR= 2.661; CI = 1.758-4.028) and sheltering the animals at night (OR= 2.448; CI = 1.568-3.882) also showed association with a positive skin test. The herd owners and animal handlers had poor knowledge regarding signs, symptoms and the zoonoti...
Qudrat Ullah1,*, Huma Jamil1, Zafar Iqbal Qureshi1, Muhammad Saqib2 and Heinrich Neubauer3
...n the serum. Serological analysis revealed overall flock-level sero-prevalence of 92.3% which varied from 88.88% in sheep and 100% in goat, while individual level sero-prevalence was 15.6% in sheep and 15.0% in goats, the difference being non-significant. A significant (P<0.05) association was found between seropositivity against C. burnetii and variables like livestock farm, type of farming, region (locality of farm), presence of ticks, anima...
Moorthy S. Muthuswamy
...to the phenomenon. A new analysis of a 2013 Pew Research Center report suggests that sharia’s popularity in a community can make religious leaders and their radical ideas highly influential, while in communities where sharia has little influence, religious leaders and their radical ideas likewise lack influence. This article suggests the following primary explanation of the phenomenon: backed by the prestige and resources of Saudi Arabia, religious leade...

Arshad Iqbal1*, Syed Mehar Ali Shah1, Hidayat ur Rahman1, Faiza Aman2 and Aziz-ur-Rahman

Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...OS. Based on biochemical analysis of the above, results showed that O2- production could be induced significantly after cold stress at 15°C and heat stress above 30°C. SOD activity showed a similar changing profile as the concentration of O2- after thermal stress between 15°C and 30°C, and GSH concentration increased significantly under both high and low temperature. The NOS activity and concentration...
Yuan Li1, Liyan Zhang2, Karhoe Loh3, Ji Feng1, Xinqing Zheng1, Puqing Song1 and Longshan Lin1,*
...A historical demographic analysis indicated that P. minor experienced a recent population expansion during the late Pleistocene period. Due to the need of P. minor to adapt to the diverse habitats, unique haplotypes were ultimately formed under the differing pressures of natural selection. This study is expected to provide a basis for future research of the population genetics of P. minor.
...

Ali Muhammad*, Yasser Durrani, Majid Suhail Hashmi, Ihsan Mabood Qazi, Muhammad Ayub and Saifullah 

...ith NaHCO3). Statistical analysis showed that storage intervals and treatments had a significant (p<0.05) effect on physicochemical and sensory quality of neutralized whey. 

...

Shaista Naz1*, Noor Paio Khan1, Naveed Afsar2 and Ashfaq Ahmad Shah3

...d data were subjected to analysis using descriptive statistics, frequencies, percentages, and rank orders. The results revealed that women were involved in buffalo, cattle, goats and sheep rearing with higher possession of small ruminants (goats and sheep), while men were found dominant in large ruminants’ possession (buffalo and cattle). Women’s higher time allocation to livestock management activities depicted their higher level of participation ...

Monsif Ur Rehman1*, Hidayat-ur-Rahman1, Muhammad Iqbal2, Iftikhar Hussain Khalil1 and Zahir Shah3 

Sher Nawab Khan* and Ghulam Hassan 

...d parameters. In diallel analysis highly significant dominant (b) and additive (a) genetic effects were detected for all the parameters except spikelets spike-1. Scaling test for the adequacy of the model was complete to partially adequate for the studied parameters. Similarly, both dominant (H) and additive (D) genetic components were observed significant for the studied characters. Moderate to high broad and narrow sense heritability was recorded for the stu...

Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq

...in females (19.44%). For analysis of hematological parameters blood samples were collected. The mean values for hemoglobin (Hb), Packed Cell Volume (PCV), Red Blood Cells Count (RBC), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), White Blood Cells count (WBC), Heterophils (%), Eosinophils (%), Basophils(%), Monocytes(%), and Lymphocytes(%) values of negative chickens were 9.43±0.49 g/...

Waqas Ali* and Mudasser Habib 

...al strains. Phylogenetic analysis (maximum likelihood) showed that lineages Pan Asia II, An Iran-05, and Group VII within serotype O, A and Asia 1 are causing majority of the outbreaks in the Pakistan during recent years. Moreover, only one residue of VP1 region was under positive selection pressure for serotype A (position 141) located in known critical antigenic region but as a whole protein was under negative selection pressure for serotype O, A and Asia 1....
Fehmeeda Fatima, Asif Nadeem* and Maryam Javed
... the results of homology analysis showed maximum similarity with sequences of goat and sheep sequences (partial) and were found to be 99.93% and 99.35%, respectively which was further confirmed by constructing phylogenetic tree which indicated that Sindh ibex, domestic goat, and sheep share a common ancestor.
...
Muhammad Babar Khawar1, Muddasir Hassan Abbasi2*, Zillay Mariam1, Nadeem Sheikh1,2*
...e way ANOVA. Serological analysis of both Group 1 and Group 2 showed a significant decrease of various components including Cl- (P=0.0023), K+ (P=0.0002), Na+ (P=0.0001), total proteins (P<0.0001) and albumin (P<0.0001) when compared against control. Similarly, hematological analysis revealed a significant decrease in WBCs (P<0.0001), RBCs (P=0.0001), Hemoglobin (P<0.0001), Platel...
Tahira Batool1*, Nabila Roohi1, Athar Mahmud
... analyzed by statistical analysis system (SAS, 9.1) software through factorial Analysis of Variance technique (ANOVA) and means were compared by Duncan’s Multiple Range (DMR) test. The findings of this study revealed L3 lysine regimen to be the best for improving the slaughter characteristics including weight of head, shanks, lungs, liver, gizzard, heart, and carcass yield comprising percent weight of breast and thigh....
Ghazunfar Rashid1, Muhammad Avais1, Syed Saleem Ahmad1, Muhammad Hassan Mushtaq2, Rais Ahmed3,*, Mahboob Ali1, Muhammad Naveed-ul-Haque4, Mehtab Ahmad5Mumtaz Ali Khan1 and Naimat Ullah Khan6
...dders; Spectrophotometry analysis of blood samples from these animals showed abnormally high levels of nitrite. In conclusion, nitrate contents were higher (>5000 ppm) in stem parts of common livestock fodder harvested in early morning and therefore, high level of nitrite found in blood of animals fed with the fodder containing high nitrate contents. This high level of nitrates in fodder crops constitutes a threat to the health and productivity of dairy ani...
Muhammad Mohsin1, Dai Guilin1,*, Chen Zhuo1, Yin Hengbin1 and Muhammad Noman2
...nd effort data. For data analysis, two specialized fishery software CEDA (catch and effort data analysis) and ASPIC (a stock production model incorporating covariates) were employed. Three surplus production models (SPMs) viz., Fox Model (FM), Schaefer Model (SM) and Pella-Tomlinson Model (PTM) were used in CEDA along with three error assumptions (EAs) viz., normal error assumption (NEA), log-normal error assum...
Saba Irshad*, Aruba Muhammad, Ammara Muazzam, Farah Sarfraz Anmol and Rehman Shahzad
... patients. Hematological analysis of thalassemic patients revealed low Hb, WBCs and platelets counts in contrast to control. The reported polymorphism was meant to be lowering the frequency of blood transfusions and to some extent responsible for diminishing the disease burden among ‘Thalassemia Major’ patients. 
...

Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3  

... in vitro. Phytochemical analysis reveals that the methanolic fraction of oil possesses metabolites like carbohydrates, amino acids, alkaloids, flavonoids and glycosides. To test the antibacterial activity of the extracted seed oil, Agar well diffusion assay was employed. Effectiveness of the oil against selected bacterial species was measured in terms of zone of inhibition in millimeters. Amoxicillin was used as a positive control. The results showed that Cla...
Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*
...-probability statistical analysis. Results indicated that an overall 63.28% (243/384) of goats were found positive for SCM, of which 58.85% (143/243) revealed Staphylococcal growth among them 69.93% (100/143) were CPS, while 30.07% (43/143) were found as CNS. Drug susceptibility against penicillins, cephalosporins, macrolides and other drugs showed that 50% of CPS and 30% of CNS were found multi-drug resistant-exhibiting resistance against more than two...

 Sadia Murawwat1, *Nageen Fatima1, Qandeel Iqbal1, Syeda Ameena Rizvi1 and Mehak Fatima1

ELECTRICAL ENGINEERING BEYOND CLASSROOM: CHALLENGES AND ANTICIPATED RESOLUTIONS
...cation in Pakistan. Data analysis imitates following problems relating to EE: dearth of professionalism in academia, insufficient educational capitals, not as much of hardware research laboratories at post graduate level, insufficient practical trainings, internships and professional skill development for improvement of hands on experience. These factors influence an Engineering graduate even after the completion of their undergraduate studies. After sorting t...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
...ibiogram and resistogram analysis were carried out through agar disc and agar well diffusion methods. Optimum temperature for growth of all bacterial strains was 37oC and pH 7 except Salmonella which had pH 6. Among all the antibiotics Oxacillin was less potent as three bacterial strains showed resistance against it while others antibiotics were more potent. Minimum inhibitory concentration of heavy metals for all bacterial isolates was different. It was concl...

 Sumera Siddique1,†, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1*, Amtul Bari Tabinda2, Muhammad Irfan1

Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
...onditions. The proximate analysis revealed that the water melon peels showed the highest amount of crude protein (which was measured by micro Kjeldahl digestion system) followed by bagasse, mango, and apple peels with respective values of as 13.38%, 3.20%, 2.69% and 2.58%. Best growth of C. tropicalis SS1 was obtained in aqueous extracts of 2% WM peels and the yeast cells attained up to 33% protein content on dry weight basis. In 2% aqueous extract of WM, the ...

Muhammad Babar Khawar, Nadeem Sheikh*

Effect of paper industry leachate on various serological indices and serum proteins of wistar rats
...nd processed for further analysis. Serological analysis revealed that leachate induction leads to significant positive variations in level of serum aspartate aminotransferases (AST) (P<0.0001), cholesterol (P<0.0001) and High density lipoproteins (HDL) (P<0.0001) while a significant negative change in triglycerides (P=0.0002) and creatinine (P=0.0370) level in both experimental groups. Alanine aminotransferases (ALT...

 Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2

Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...al and histopathological analysis.Cr+6 treated group showed severe histological changes like necrosis, cirrhosis and dehydration in liver evident by significant elevation of Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT), Alkaline Phosphatase (ALP), total protein, bilirubin, globulin, creatine and uric acid along with reduction of SGOT/SGPT, Blood Urea Nitrogen (BUN), urea and albumin as compared to control. Treatmen...

Tasleem Akhtar, Nadeem Sheikh*

Induction of acute phase in response to tacrolimus induced hepatotoxicity
...imus dose. Densitometric analysis revealed considerable elevated level of some positive acute phase proteins, specifically C-reactive protein, haptoglobin and ceruloplasmin. While albumin and transferrin levels were found to be low as compared to control animals. The results obtained in this study revealed that tacrolimus resulted in the onset of acute phase response in experimental animals owing to its toxic potential.

...

Durr-e- Samin Tahir*, Waqar Nasir, Sarah Bushra, Fatima Batool

Height trends in the population of Rabwah, district Chiniot, Pakistan and comparison with WHO standards
...lso recorded for further analysis. The R- Programming and
smoothing spline technique were applied to analyze the height trends of the collected data. The average height of
male adults is found to be 172cm (67.71" ± 3.98) and for females it is159 cm (62.55" ± 3.06).Comparison of male and
female height trend indicates that growth rate is almost the same up to 13 years, but it is higher for males after 13
years ...

Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*

Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
...trol.
Comparative analysis between experimental and control samples revealed protein fractions (KDa) of 149, 132, 101,
72, 75 and (163, 114, 91, 75, 72, respectively. Conclusively, it can be stated that extensive use of BPA in various
application cause serious health hazards, as noted by changes in serum proteins and transaminases. Therefore,
further investigation of its toxicity is necessary in order to consider it safe.

...

 Jhan Zeb, Muhammad Javed

Forecasting percentage contribution of plankton biomass towards increase in fish yield under composite culture conditions
... subjected to regression analysis in order to estimate the percentage contribution of
plankton biomass towards increase in fish yield. The correlation for plankton productivity v/s increase in fish yield was
positively significant (p<0.01) under all the treatments, showing the direct dependence of fish yield on plankton
productivity. The order of percentage contribution of plankton productivity towards increase in fish yield is T3 ≥ ...

 Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3

Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...y and
biochemical analysis [alanine amino transferase (ALT), and aspartate amino-transferase (AST)]. Liver histology
revealed that necrosis, nuclear pycnosis, hypertrophy of hepatocytes, vacuolization, nuclear atrophy and congestion
of blood vessels were observed as compare to control group. This result was also supported by the significant
increase in levels of hepatic enzymes AST and ALT in blood plasma of exposed fish as compared...

Sadaf Niaz1, Masroor Ellahi Babar2, Tanveer Hussain2, Asif Nadeem1, Misbah Hussain1, Riffat Mehboob3*, Fridoon Jawad Ahmad3

Mutation analysis of RING1 domain of Parkin in early onset of Parkinson’s disease in Pakistani patients-a pilot study
...study was to do mutation analysis of RING domain of Parkin
gene as there is no mutation reported previously. In current study, sequence analysis of RING1 domain of Parkin
protein was performed in a sample set of 30 patients (selected from different areas of Punjab, Pakistan) to find out
any Single Nucleotide Polymorphism (SNP).No SNP was detected in RING1 domain that could be related to the
diseas...

 Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1

Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
...aminases activity
analysis. TAA injection resulted in significant alterations in serum proteins and transaminases levels. Significant
hypoproteinemia and hypoalbuminemia were observed in sera as well as liver tissue of both experimental groups
compared to control. Furthermore, transaminases activity was significantly increased for both; AST (P=0.0045) and
ALT (P=0.0015) in both experimental groups when compared to control. Taking in...

 Zubair Ahmed#, Muhammad Farhanullah Khan, Habiballah Rana*

Toxicological effects of Haloxylon recurvum Bunge ex Boiss (Khar Boti) whole plant extract and novel insecticide chlorantraniliprole against maize weevil, Sitophilus zeamais Motschulsky
...ts in S. zeamais. Probit analysis revealed LD50 value for H.
recurvum plant extract were 16.47 μg/insect and chlorantraniliprole were 0.0358
μg/insect, after 24 hours. The result revealed that H. recurvum plant extract had
insecticidal potential against the stored grain pest, S. zeamais. Current study
suggests H. recurvum plant extract could be a good alternative against
conventional insecticides.

...

Madeeha Arshad, Asmatullah, Chaman Ara

Embryotoxic effects of sodium arsenate in Mus musculus
... and histological
analysis. Morphometric observations indicated significant (P≤0.05) variations
among groups in certain parameters (body weights, crown rump length, head and
eye circumferences) against control. Microcephaly, hygroma, skin hemorrhages,
scoliosis and appendage deformities among arsenic treated groups were observed.
Histological studies revealed abnormalities like spina bifida, cardiac malformations,
m...

Nabila Roohi, Mehjabeen, Samina Ashraf*

Effects of cigarette smoking on serum proteins profile in male active and passive smokers
...ent study deals with the analysis of serum total proteins and fractions
(albumin, total globulins, gamma globulins and non-gamma globulins) of active and
passive smokers. Blood samples of 180 cigarette smokers were collected from
different locations of Lahore and 60 healthy non-smokers from University of the
Punjab, Quaid-e-Azam campus Lahore, in terms of comparable age, height, weight
and socioeconomic set up. Serum protein ...

 Anber Naqvi1*#, Abdul Qadir1, Adeel Mahmood2, Mehvish Mumtaz3, Iqra Aslam1, Gan Zhang4

Assessment of polychlorinated biphenyls (PCBs) in maternal blood serum from selected districts of Punjab, Pakistan

 Abdul Majid Khan*

Review report on mammalian dental defect (enamel hypoplasia) in different groups of mammals
... /> map for comparative analysis of dental health in different mammalian taxons.

...

Khush Bukhat Zahid1*, Ghaffar Ali2, Samina Sabir1 and Mohammad Fayaz2 

...tural productivity. This analysis is useful from the stand point of policy makers and analysts as it will help them develop policies relating to improvement in agricultural sector in the context of climate change. 

...

Abid Muhammad Shah1, Safdar Ali1*, Ijaz Ahmad2, Gulzada Wazir1, Obaidullah Shafique1, Muhammad Amir Hanif1, Bashir Ahmad Khan3 and Sumaira Zareen4 

...e analyzed using Fishers analysis of variance technique and means were compared by using the least significant differences test (LSD) at 5% level of significance. Most of the studied parameters showed significant results (P≤0.05) for yield and weed indices. The results showed that highest weed control efficiency (41.86%) was recorded for hand weeding followed by Ally Max (30.8%) which were significantly different from each other; while, in case of sowing me...
Faiz Muhammad1,2, Canfeng Dou1, Zhen-ming Lü1,*, Li Gong1, Xun Du1 and Muhammad Shafi3
...bor-joining phylogenetic analysis revealed three lineages. The Dongshan population distinctly has shown separate lineage. The haplotype networking has shown that Hap1 contributed 48.051% and appeared in six populations whereas, Hap6, Hap11, Hap 16 and Hap17 were also shared by some populations, rest of the haplotypes were unique. This study solely provide species specific information on its genetic structure and population variability in Chinese waters.
...
Jun Yan Bai1,*, Yong Gang Zhao2 and Yu Qin Wang1
... for further correlation analysis with growth indexes of goats. 
...

Muhammad Shahzad Khattak1*, Amjad Khan1, Mahmood Alam Khan1, Waqas Ahmad2, Shafiq ur Rehman3, Mohammad Sharif4 and Sajjad Ahmad1 

...ere also detected in the analysis period. Several strategies for the mitigation of adverse impacts of droughts in the study region are recommended. 

...
Farhat Iqbal1, Mohammad Masood Tariq2,*, Ecevit Eyduran3, Zil-e-Huma4, Abdul Waheed5, Farhat Abbas2, Muhammad Ali2, Nadeem Rashid2, Majed Rafeeq2, Asadullah2 and Zahid Mustafa2
... deals with the Bayesian analysis as an alternative to the classical approach for estimating the body growth of Mengali sheep breed of Balochistan, Pakistan. The parameters mature weight, integration constant, maturity rate and their credible intervals of four widely-used nonlinear sigmoidal growth models were estimated through Bayesian inference. Gompertz, Logistic, Brody and Von Bertalanffy models were fitted to average monthly body weight data of (n = 412) ...
Xiao-Ying Ren1, Di Zhang2 and Wan-Long Zhu1,*
...es, principal components analysis, thin plate spline, UPGMA and Multidimensional Scaling were used. The results showed that there had significant differences in skull shapes of centroid size in Eothenomys. Orbital cavity and alveolar region had significant differences in intraspecific skulls, some differences were shown in interspecific skulls, such as the point between the anterior tip of suture nasal and premaxilla, anterior tip of suture between nasa...

Mujib ur Rahman1, Munir Khan2*, Himayatullah Khan2, Malik Muhammad Shafi2 and Haidar Ali2  

... selected. A comparative analysis of chadar and suit cloth was done regarding sale in Charsadda market and other cities of the country. In Charsadda market, 3/4 producing units sold their products, while 1/4 of the producers were involved in marketing of these products to other cities of the country. Profit per month of Chadar and Suit cloth in the local market was found Rs. 7,800/- and Rs 6,552/-, respectively. While selling these products in other cities per...

Muhamad Jawad*, Shahid Riaz Malik, Muhammad Aqeel Sarwar, Muhammad Asadullah, Israr Hussain and Rabia Khalid 

...e genotypes. Correlation analysis indicates negative correlation of yield with lowest pod height and highly significant correlation between lowest pod height and plant height. Cluster analysis performed for qualitative and quantitative traits separately. It was observed that genotypes with suitable plant type for mechanized harvesting belongs to different cluster and high yielding genotypes belongs to another cluster. It ind...

Sahar Shibli1,2*, Farzana Siddique1, Saeeda Raza2, Zaheer Ahsan3 and Irum Raza4 

...plusmn; % fiber. Mineral analysis of peanut cultivars showed 12.60±0.38 to 16.61±1.51 mg/100g Fe , 2.34±0.075 to 3.37±0.040 mg/100g Zn, 38.64±3.50 to 48.24±32.58 mg/100g Ca, 67.81±7.86 to 82.72±9.09 mg/100g Mg, 199.19±33.18 to 342.00±19.03 mg/100g Na and 1220.6±9.045 to 1411.3±1.71 mg/100g P and 841.01±50.41 to 992.98±36.10 mg/100g K. Fatty acid characterization o...
Mahboob Ali1, Muhammad Avais1, Muhammad Ijaz1, Memoona Chaudhary2, Riaz Hussain3, Amjad Islam Aqib1, Naimat Ullah Khan4, Muhammad Luqman Sohail5,*, Madiha Khan6, Mumtaz Ali Khan1, Mehtab Ahmad7, Muhammad Sajid Hasni2Imran Qaiser8, Ghazanfar Rashid1, Ikramul Haq1 and Ikramullah Khan8
...al mastitis. Statistical analysis revealed non-significant (P>0.05) difference between Cholistan desert and Suleiman mountain range (48.18% and 46.09%, respectively). S. aureus was isolated from 53.03% of the milk samples, with significantly higher (P<0.05) prevalence in Cholistan desert (66.10%) as compared to Suleiman range (57.98%). Blind quarter prevalence of S. aureus subclinical mastitis was 8.46% and 11.59% (P...
Muhammad Furqan1, Usman Ali1,*, Anjum Ara1, Muhammad Mudassar Shahzad2, Muhammad Siddique Awan3, Riaz Aziz Minhas3, Khawaja Basharat Ahmad3, Qamar Zaman Qamar4 and Naeem Iftikhar Dar5

Babar Tasim1, Tariq Masood1*, Zafar Ali Shah1, Muhammad Arif2, Ata Ullah1, Ghazal Miraj3 and Muhammad Samiullah4 

...ard methods. Statistical analysis of the data revealed significant difference for proximate composition, pH, EC and macro and micro nutrients. Biochar obtained from Sesbania stem was found high in fixed carbon content (66.9%) and volatile matter (27%) but low in ash content (4.5%). AD biochar contained lowest amount of fixed carbon (49%) and volatile matter (17%) but highest ash content (30%) among different biochar samples under investigation. All the samples...

Zakiullah1, Muhammad Farid Khan1*, Muhammad Mohibullah3, Muhammad Iqbal2, Irfanullah3, Faheemullah3, Madiha Urooj1 and Uzma Arif1 

Syeda Beenish Bukhari1, Sidra Khalid1*, Shahid Bashir1, Riffat Mehboob2, Humaira Waseem2

Shumaila Nazeer1, Iqbal Javed1*, Allah Bakhsh2, Mudassar Yasin2, Haroon Javaid3 and Kaniz Fatima1 

...Along with export margin analysis of both markets, an effort is made to quantify impact of the major determinants on export of mandarin (Kinnow) to UAE and USA. According to data properties and suitability, multiple regression analysis technique is used for UAE and binary logistic regression technique was used for USA market. According to the finding, gross export margin for USA market is Rs. 30644 per ton and percent export...
Tamoor Azeem1,*, M. Yasin Tipu1, Asim Aslam1, Sajjad Ahmed2, Salman Ahmed Abid1, Abdullah Iqbal3, Naeem Akhtar4, Muhammad Saleem4, Aamerzish Mushtaq4 and Sajid Umar4
...d for haematobiochemical analysis and thin smear stained with Field stain according to standard procedure. Out of 200 camels 46% of them have blood parasites. Trypanosoma was the most prevalent (32.5%) followed by diptelonemiasis (8%). Babesia and Anaplasma was seen in 3% camels. Prevalence of diptelonemiasis was significantly higher in young than older camels. Blood analysis revealed that blood parasite...
Muhammad Huzaifa Mehmood1, Muhammad Ahmad Iqbal2, Muhammad Daood1, Muhammad Rizwan Tariq3*, Khubaib Ali3
...cid and physico-chemical analysis were performed. The pH texture, fat, protein, blood lipid profile and PUFA concentration was significantly affected by the incorporation of oat in rabbit feed. It was concluded that the supplementation of 2% oat in rabbit feed increase n-3 PUFA in meat while improvement in serum lipid profile was observed by 4% supplementation of oat seeds. It was also observed that fat percentage in meat was also reduced by oat seed supplemen...

Muhammad Amin1, Aftab Ahmad Khan2, Abida Perveen1, Zareen Rauf1, Sher Shah Hassan2*, Muhammad Arif Goheer2 and Muhammad Ijaz2 

...r year 2000, the spatial analysis of DEVNDVI showed that there were the mild drought conditions during the month of February and intense drought conditions in the months of March and April. During the months of May to July, the eastern and western boundaries of the study area showed the development of drought conditions and these conditions become more intense in June. From August to October, the drought also advances in towards North side. In months of Novemb...
Pingping Cang1, Mingming Zhang1, Guo Qiao1,Qirui Sun1, Dehai Xu3, Qiang Li1, Xinghua Yuan4 and Wenbin Liu2,
.... Economic profitability analysis included analysis on gross revenue, break-even production, net present value, payback time, degree of operating leverage, and environmental costs under experimental conditions was conducted. Analysis results revealed that BFT system was more effective than the control system with water exchange in gibel carp culture, suggesting that BFT system could be suc...
Zhen-li Li1,2, Hui-fang Zhou2,*, Bo-ru Zhou2, Bei Liu2, Xiao-fei Jiang2 and Jian-ya Xu2

 

...by qPCR and western blot analysis. Simultaneously, transcription factors, such as c-Jun, Elk-1, Egr-1, Nur77, CREB, and transduction molecules in PKC-MAPK, Ca2+-CAM, and cAMP-PKA signaling pathways, such as PKC, p38mapk, ERK, CAM, and PKA were assessed by real-time PCR and western blot analysis. BSZYF increased the serum FSH levels suppressed by mifepristone (P<0.05). Moreover, the expression of c-Jun, Elk-1, Egr-1, CREB,...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Nosheen Fatima1, Raheel Babar3 and Ikramul Haq4  

...tain it. The statistical analysis showed weak significant association between factors responsible for biodiversity loss and two types of biodiversity since χ2 (3) = 11.338, p = 0.010 and concluded that all factors are not equally responsible for loss in both types of biodiversity. Results showed that biodiversity conservation has its role in agricultural development. By conserving biodiversity, agricultural production can be increased. The result...

Aisha Siddiqua1*, Munir Ahmad1 and Nusrat Habib2 

...perform a counterfactual analysis and Multinomial Endogenous Switching (MES) regression model to analyze and compare the impact of different adaptation strategies in isolation or in combination on yield. The primary data of ‘Climate Change Impact Survey 2012-13’ used for this purpose. Crop-specific strategies of change in the time of sowing, use of inorganic fertilizer, change in irrigation, and varietal change. Change in irrigation practice gave r...

Syed Amjad Kamal Jan* and Naushad Khan 

...ction were used for data analysis. The results indicate that all rice grower were literate while the secondary level was more than the other level, followed by intermediate level. Similarly, in category of family size the number in above 15 category was found more than the other categories followed by 8-15 category. The Cob Douglass Production function result indicate the constant value 5.434 and found significant which shows that if other variables remove fro...

Sonia1, Khuram Nawaz Sadozai1*, Noor Paio Khan2, Abbas Ullah Jan1 and Gulnaz Hameed3 

...Model” for further analysis. Major estimates of the overall 30 years data reveal that among non-climatic variables, area under wheat cultivation and seed are highly significant to the wheat yield at 1 percent level of significance. Whereas, contribution of fertilizer to wheat crop in KP province was observed insignificant. Furthermore, the computation of climatic variables depicts that temperature contributes significantly but has inverse association wit...

Ali Awais, Charassri Nualsri and Watcharin Soonsuwon* 

... subjected to regression analysis and effective doses of EMS were recorded for both genotypes. The effective value calculated for shoot and root length in Dawk Pa-yawm was 1.34% and 1.33% and in Dawk Kha 50, 1.23% and 1.86% respectively based on the reduction of the above mentioned parameters after the EMS treatment. Furthermore, the EMS response in M1 generation was also observed in the field condition by various quantitative measurements. The traits, especia...

Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1

...vely. Three weeks’ analysis of treated mungbean leaves with SA and BTH exhibited peak accumulation of phenols and defense related enzymatic antioxidants. Highest enzymatic activity was observed in treated plants followed by inoculation with MYMV. Increase in resistance by the application of SA and BTH, antioxidant enzymatic activities of SOD, POD, CAT were also increased for second week. This revealed that SA and BTH can be used as a potential source for...

Hina Ali*, Iram Shaheen and Feroza Hamid Wattoo 

...o-chemical and microbial analysis of drinking water samples of different areas of Rawalpindi. The water samples were collected into sterile containers from the different localities of Rawalpindi such as Dhokekala khan, Morgah, Scheme-III, Gulshandadan khan, Satellite town, Dhoke kashmirian, Pandora, Bahria town, University campus (Mehr water) and Air Port. The pH of PMAS-AAUR campus water ( Mehr Water) was found within WHO recommended values (pH limit 6.5-8.5)...
Jam Nazeer Ahmad1,2,*, Muhammad Jafir1, Muhammad Wajid Javed1, Sumaira Maqsood3 and Samina J.N. Ahmad1,2,*
... in Punjab. Phylogenetic analysis was also complemented to differentiate DCB identified from other countries of the world. The PCR bands obtained in gel electrophoresis amplified PCR fragments from all DCB species at 710 bp. The sequencing results and phylogenetic analysis with sequences submitted at NCBI database revealed that DCB have 99-100% similarity with Oxycarenus hyalinipennis OH1 (JQ342987.1), Oxycarenus h...

Rehmat Ullah* and Khalid Nawab 

...nary logistic regression analysis depicted highly significant negative association between adverse effects of pesticides like headache, dizziness, feeling weak, difficulty in seeing, chest pain, burning sensation, and fever with precautionary measures/Personal Protection Equipment’s recommending that these precautionary measures and PPEs must be adopted to avoid from these health concerns. 

...

Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1 

...s. However, phylogenetic analysis revealed clustering of all tested strains within sub-genotype VIg. Three isolates were assessed for pathogenicity based upon the mean death time (MDT), the intracerebral pathogenicity index (ICPI) and the intravenous pathogenicity index (IVPI) in chicken. The ICPI and MDT revealed that all tested isolates were of moderate virulence (Mesogenic) in chickens. Mature chickens showed no clinical signs or death as assessed by IVPI. ...
Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu
...antitative real-time PCR analysis indicated that the duck ChREBP mRNA is widely expressed in all examined tissues. ChREBP mRNA level was the highest in abdominal fat and the lowest in gizzard. The g.247075G>A silent mutation in exon 10 was first identified by direct sequencing approach, and resulted in three genotypes of AA, GA and GG. Association analysis demonstrated that the liver ChREBP mRNA expression level was signi...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
...=0.013). Mantel-Haenszel analysis showed that MTHFR polymorphisms confounded the effect of maternal age CHD in DS. MTHFR polymorphisms appear to be risk factors for CHD in DS.
...
Rishen Liang*, Meng Zhou, Zhenxiang Lin, Guozhang Li, Yuan Chen, Xuan Lin and Zaohe Wu
...tebrates. By comparative analysis of the two genomes, 941 variable sites (5.69%) were found. Sequence divergences of 13 protein-coding genes, 2 rRNA genes and one control region which are commonly used as molecular markers between the two species ranged from 2.4% (12S rRNA) to 11.2% (ND6), all divergence values were larger than 2%. Great variations of Cyt b and COI between P. orientalis and P. vittatus were revealed to be 6.0% and 7.4%, respectiv...

Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*  

...ively. The phytochemical analysis of leaves and roots extracts revealed the presence of alkaloids, saponins, cardiac glycosides, tannins, phenols and flavonoids etc. It is concluded that S. saligna crude extracts as well as crude saponins exhibit broad antimicrobial spectrum against various disease-causing microbes. 

...
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
...g to the findings of the analysis, it was seen that January, March, October, November and December are the months when production performance is high. When these months were ranked among themselves, it was observed that January, March and November are the first three ranks, respectively, and that the rank was also supported by the combined results of different techniques. As a result, in terms of production performance for the enterprise, it can be said that t...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...ulase activity. SDS-PAGE analysis confirmed the molecular weight of recombinant protein as 39 kDa. The protein was found thermostable which retained more than 70% residual activity with an incubation of 1.67h at 90 °C in the presence of cobalt. Presence of ionic and non-ionic detergents showed an inhibitory effect on the enzyme activity. Kinetic studies of recombinant protein demonstrated the km and Vmax values of 0.22 m...
Liyan Zhang1,2, Zhidong Zhou1,3, Haiping Li1,2, Yanlong Qiao4, 5 and Yueping Zhang1,3,*
...ty and genetic structure analysis of S. japonicus showed that these four populations exhibited moderate genetic diversity across the sampled geographical range of the species. However, no phylogenetic structure corresponding to geographic location was observed from the neighbor-joining (NJ) tree and haplotype network analyses, and gene flow and exact P test results revealed that a wide range of gene flow occurred among the four S. japonicus
Marian Kuczaj1, Ewa Czerniawska-Piątkowska2,*, Alicja Mizera1, Renata Pilarczyk2, Bogumiła Pilarczyk3 and Patryk Barton1
...ose of the paper was the analysis of the impact of selected breeding and environmental factors on the results of rearing heifers of the phf red-white variety. The research included 133 cows and heifers and their offspring, i.e. 116 calves only from single pregnancies. The calves were weighed and the zoometric measurements were carried out on the day of birth, at the age of 1 month and 6 months, and daily increments were calculated in the analyzed period...
Zhen-Yang Wu1, Guang-Xin E2,*, Hui Ran1, Da-Hui Wang1 and Tian-You Yang1
...opulations. Phylogenetic analysis revealed that these 10 populations could be separated into two clusters: the first included CD, WS, YP, JK, and ST, and the second included DJ, YJ, YH, SQ, and SN. However, there was no significant divergence between populations based on pair-wise differences (FST). Overall, the 10 populations were divided into two subgroups and two D-Loop haplotype clusters. This study showed that the different rice frog pop...

Dilshad Ahmad1* and Muhammad Afzal2

...s employed for empirical analysis and multiple stages random sampling technique used for data collection of 400 Bt and Non-Bt cotton farmers for the year of 2012-13. The results of the study have indicated Bt cotton farmers of both districts with higher technical efficiency score as more efficient than Non-Bt cotton farmer. Empirical findings specified a cropped area, seed, labor, fertilizer and irrigation were the significant variable of increasing cotton pro...

Irfan Ullah*, Abbas Ullah Jan, Muhammad Fayaz, Amjad Ali and Atta Ullah Shah 

...tics were considered for analysis. The uncompensated own price elasticity for milk, fruits, and baked products is greater than unity which suggests that these food groups are highly responsive to their own price change. Prices of these food products should be kept constant. Imposition of any sale tax could create a huge loss in consumption for these commodities. Milk, meat, fruits, other cereals, tea and coffee and baked products were treated as luxuries by th...

 Adile Tatliyer1,*, Sinan Bas1 and Serdar Yagci2

 

...tability of factor score analysis. A result showed that implementation of factor analysis was appropriate for the studied data set at each period. At each period, factors whose eigenvalues which is the characteristic roots of a square matrix with p×p were higher than 1 were elected by applying the Varimax rotation in explanatory factor analysis. In the first period...

Khadim Hussain Wagan1*, Muhammad Ibrahim Khaskheli2, Jamal-U-Ddin Hajano1 and Abdul Ghani Lanjar2 

...equence and phylogenetic analysis. There was significant variation (Df= 54, F=7.4, P=0.0000) among cfu per gram of M. phaseolina in soil samples collected during field survey at different districts of Sindh during 2015 and 2016 growing year. In soil sample maximum 41.371 cfu per gram of M. phaseolina with range of 27.00-48.40 were counted from Badin during 2016 followed by 40.2 with range of 27.00-45.20 during 2015 growing season. The plant tissues were carryi...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

...Ct method. Further analysis was done by SPSS, medcalc and Graphpad prism software. Area under curve analysis was used to get diagnosis outcome of selected miRNA panel. Upregulation of miRNA panel (mir-376c, mir-155, mir-17a and mir-10b) has been seen in the plasma samples of TNBC patient´s samples relative to non-TNBC control samples. The scatter plots and other statistical analysis

 Asad Sultan1, Rabia Ali1, Rifat Ullah Khan2,*, Sarzamin Khan1, Naila Chand1 and Ambrina Tariq3

...ted to measure proximate analysis, minerals and phytate content in grain and fecal samples. Sorghum cultivars were different in nutrient profile with red higher in protein content (11.41%). It was observed that phytase inclusion in grain increased the availability of all nutrients except crude lipids. Total tract nitrogen retention was increased by 3% in red sorghum compared to white. Minerals absorption was increased but differently in different cultivars wit...

Ihsan Ullah Khan*, Muhammad Arshad, Muhammad Ayub Khan, Muhammad Ashraf, Ashiq Saleem, Sundas Awan, Samra Azam and Shamim Ul Sibtain Shah 

Bibi Nazia Murtaza1,2, Azhar Qayum3, Shamaila Inayat Nadeem1, Naif Awdh Al-Maliki4, Abdulaziz Alamri4 and Abdul Rauf Shakoori2,5,*
... determined by in silico analysis and molecular docking of variants with GTP. MutationTaster was used for functional analysis of genetic variants and three-dimensional structure of mutant proteins were built by Swiss-Model and were further subjected to structural alignment and stability studies by I-Mutant Suite and DUET server. Both variants were predicted as ‘disease causing’ and protein stability
Faiza Jabeen1,*, Bushra Muneer2 and Javed Iqbal Qazi3
...and starch. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates belong to the same specie Anoxybacillus rupiensis with 99% similarity under Accession number KF254911 (JF82) and KC849452 (JF83). The DNA G + C contents were 56.854% and 56.754% for A. rupiensis JF82 and A. rupiensis JF83, respectively. The isolates grew over a wide range of temperatures (40-70°C) and pH (4-9). The isolates exhibited growth w...
Zhaochao Deng1, Jingchen Chen1, Na Song2, Yongzhen Li3 and Zhiqiang Han1,*
...etic structure. However, analysis of this structure has not included the South China Sea population. In the present study, we collected a total of 23 individuals from Xisha, and Nansha Islands in the South China Sea and a 503 bp segment of mitochondrial DNA control region was sequenced. 17 polymorphic sites were obtained defining 7 haplotypes in the South China Sea population. Contrary to heterogeneous expectation, AMOVA and pairwise FST valu...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
... mutations. Phylogenetic analysis by MEGA 7.0 revealed that the evolution of the tested genes is consistent with the evolution of the tested birds. The results of the selection stress test show that only a few sites in the GED region of the Mx gene are positively selected during evolution, and the majority of the amino acid sites are constrained by the strong structure and function of the protein, indicating a high risk of avian influenza infection in t...
Ashfaque Ahmed Nahiyoon1,*, Shahina Fayyaz2 and Nasira Kazi2
... and Tando Allahyar. The analysis of samples resulted in the identification of one new species of soil nematode viz., Acrobeloides gossypii n. sp., along with three new record species viz., Tylenchorhynchus ewingi Hopper, 1959; T. crassicaudatus Williams, 1960 and Pratylenchus pseudofallax Café-Filho & Huang, 1989. Description, measurements and illustrations of these species are incorporated herein.
...
Tianlong Cai1, Falk Huettmann2, Kisup Lee3 and Yumin Guo1,*
...longjiang River. The gap analysis revealed that existing nature reserves conserve merely 16.7% of the suitable habitat and 26.7% of the core habitat. However, much more suitable habitat is still located widely outside nature reserves. Based on our predictions, a total of 22 priority areas should be considered when developing new nature reserves or expanding the existing nature reserves. In addition, we suggest that seven regions located at the Korean Peninsula...

Qaizar Ahmed1, Fida Mohammad2, Sheraz Ahmed2*, Sultan Akbar Jadoon2, Imtiaz Ali2 and Ajmalud Din2 

...gh both models. Griffing analysis revealed additive type of gene action in the expression of all traits across all environments, except nicotine content. Contrarily, Hayman analysis identified both (additive and dominance) type of gene actions for yield and other traits. Similarly, Hayman analysis discriminated gene actions for various traits more specifically across environments. Based on...

Gulnaz Saleem1, Aijaz Hussain Soomro1*, Nouman Rashid2 and Mehar un Nisa Narejo3 

...of the biscuits. Sensory analysis revealed that there were no significant differences (p>0.05) amongst all treatments. The study concluded that biscuits might be prepared from lentil flour especially at the level of 21 and 28% considered most excellent by the panelists with respect to all sensory attributes. The biscuits supplemented with wheat; lentil flour at all proportion ratios was found to be acceptable for sensory quality. 

...

Aziz Ur Rahman* and Syed Mehar Ali Shah 

...stan during 2017. Pooled analysis of variance indicated significant (p≤0.01) differences among the environments, genotypes and GEI for spikelets panicle-1, grains panicle-1, 1000-grain weight and grain yield. Across four locations, AUP-30 showed the highest number of spikelets panicle-1 (242.5), grains panicle-1 (228.6) and maximum 1000-grain weight (29.0 g). Rice RILs AUP-3, AUP-30 and AUP-29 manifested the higher grain yield of 4.3, 4.2 and 4.1 tonnes ha-...

Hafiz Umar Farid1, Muhammad Zubair2, Zahid Mahmood Khan1, Aamir Shakoor1*, Behzad Mustafa1, Aftab Ahmad Khan3, Muhamad Naveed Anjum4, Ijaz Ahmad5 and Muhammad Mubeen

...0.212, respectively. The analysis indicated that parameters such as farm location near the head reach, groundwater quality and long farming experience showed negative impact on adoptability of HEIS in the study area. It was also observed that high economic returns, deployment of operator, farmer’s training and presence of farmer at his farm are the gateway towards the success of these systems. The present study provides guidelines for the policy makers a...

Tariq Shah1*, Umar Hayat1, Muhammad Suleman Bacha2 and Muhammad3 

...regression were used for analysis. It is observed during the study that livestock extension department and private livestock doctors are the key sources where farmers get advice regarding livestock production. Majority of the respondents are quite satisfied regarding the effectiveness of these advices. Results further indicate that farmers visit or contact livestock expert mainly for livestock diseases and artificial insemination. However, some of the responde...

Muhammad Nasrullah1*, Liu Chang1, Khurram Nawaz Saddozai2, Asaad Osman Khalid1, Rachel Bayisenge1 and Gulnaz Hameed3 

...ollenearity problem. The analysis of profit function is prior to our expectation and economic theories. Factor affecting analysis shows that overall results of the regression are statistically significant except farm size and fertilizers used during production. The coefficients of seed are the highest among all variables which can highly affect the productivity of the growers. 

...

Asim Khan1, Shahid Ali1*, Syed Attaullah Shah1, Aftab Khan1 and Raza Ullah2 

...ctively. For statistical analysis, panel data record for twenty years (1996-2015) across five districts of the province were scrape together from Development Statistic of Khyber Pakhtunkhwa, Pakistan Bureau of Statistic and Pakistan Meteorological Department, Khyber Pakhtunkhwa. Hausman test recommended fixed effect model (as opposed to random effect) as a fit model for given estimation. The major findings proclaim that maximum temperature has negative impact ...

Abdur Rehman1*, Iqbal Javed2, Zhang Nannan3, Muhammad Niamatullah1, Raheel Saqib4 and Allah Bakhsh

...e and adopted the system analysis method to focus on the existing problems and to put forward the significant and the specific guidelines. The results showed that the Lang fang city is located between Beijing and Tianjin stands first in the comprehensive level of ranking for the development of agricultural informatisation and remained first in the ranked of index popularisation and application of agricultural informatisation. The Tangshan city ranked first for...

Abid Ali1*, Hidayat ur Rahman1, Farhatullah1 and Zahir Shah

..., 2016. Generation means analysis (GMA) indicated presence of complementary epistasis in the inheritance of ear leaf area, ear length, grain yield and harvest index. Likewise, duplicate type of epistasis was noted in the controlling of biological yield. Duplicate as well as complementary epistasis were found responsible in controlling the expression of plant and ear height, maturity and stover yield as well. Additive x dominance type of epistasis played major ...
Bingbing Gao1, Na Song1, Zhonglu Li2, Tianxiang Gao3 and Liqin Liu3,*
...ned and used for genetic analysis. No insertion or deletion were found among 26 mutation loci and 27 haplotypes were detected. All six populations were characterized by low haplotype diversity (0.39–0.59) and low nucleotide diversity (0.00038–0.00073). The result of AMOVA and pairwise Fst values indicated weak or no genetic differentiation among different populations. Haplotype network and NJ phylogenetic trees revealed that there...
Abdur Rauf Nizami*
... a detailed petrographic analysis to ascertain its faunal assemblages in the Chichali Gorge Section. These investigations revealed that the limestones of this formation are a good repository of faunal fossils. The fossil contents found in the Samana Suk Formation display a wide range of faunal species. The documented species are comprised of: foraminifers, gastropods, pelecypods, bryozoans, echinoids, sponges, brachiopods, and corals.
...
Xiaona Cao1,3-5, Yuanyuan Ren2-5, Xiaoteng Cui2-5, Baoxin Qian2-5, Chunyan Zhao 2-5, Jie Yang2-5, Chao Su2-5,* and Xingjie Gao2-5,*
Jun Yan Bai*, Shuai Yang, You Zhi Pang, Xiao Hong Wu and Guang Lu Li
...
...

Waqar Akhtar1*, Munir Ahmad2, Nadeem Akmal1, Hassnain Shah1 and Asif Ali Mirani2 

Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
...d through Epi-Data. Data analysis was performed through SPSS. Chi-square and regression analysis were conducted. An overall prevalence of 67.3% was found. On multivariable logistic regression several health (lactation stage, number of lactations, body mass index, udder shape and milk yield), management (udder preparation and teat dipping) and bio-security (source of animal, feed sharing, manure removal and de-worming) factor...
Songhao Zhang1, Yiwen Gong1, Jian Xu1, Mou Hu2, Peng Xu3 and Yanliang Jiang1,2,4,*
...omparative transcriptome analysis via high throughput sequencing, and examined transcriptome profiles of purse red carp by comparing with Yellow River carp, of which the skin color and body shape are quite similar to wild carp. A total of 434 million reads was generated, resulted in 203,590 assembled contigs, which was used as the reference sequences. Comparing the skin and blood transcriptome files showed that 2,767 unigenes were significantly differentially ...
Hashim Ullah1, Abdur Rahman1, Rifat Ullah Khan2, Shakoor Ahmad2 and Ambrina Tariq3, Shabana Naz4,*
...uality through proximate analysis. Wazirisheephad lower cholesterol as compared to Mazai. With the increasing BCS, the cholesterol content was significantly increased in both the breeds. The dressing percentage and crude protein were higher in Waziribut the fat content was found greater in Mazai. Similarly, females have higher average fat content as compared to males. In conclusion, from the health point of view, the mutton of Waziri breed had better nutrition...

Shakeel Ahmad Anjum1, Sami Ullah1*, Muhammad Mohsin Raza2, Mohsin Raza1, Muhammad Abbas3, Ijaz Ahmad4, Malik Muhammad Yousaf2, Muhammad Zeshan5, Adeel Abbas1, Mehmood Ali Noor1 and Mohsin Nawaz1 

... booting stage. Economic analysis revealed that 0.05 M B application gave highest net income and benefit-cost ratio. Thus foliar application of 0.05 M B application (foliar) at booting stage can improve wheat productivity possibly by optimizing post-vegetative plant metabolic activities during grain filling, in contrast to late application rate. 

...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...d nucleic acids. The PCA analysis also exhibited that these three groups were well distributed in different areas. In conclusion, the microoperation of ICSI caused some changes in the metabolism of embryos. Raman spectroscopy is a valuable technology for assessing embryonic metabolism.
...
Rabia Khalid1, Saleema Bashir Shams1, Bibi Nazia Murtaza1,*, Gaitee Joshua1, Saira Mushtaq1, Hassan Al-Talhi2 and Abdulaziz Al-Amri2

Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3* 

...identified. The sequence analysis of BnSHP1-like and BnSHP2-like demonstrated that these genes are 747 bp and 735 bp in size, respectively. The nucleotide alignments revealed 98% identity of BnSHP1-like and BnSHP2-like with BnSHP1 and BnSHP2 sequences. The sequence homology was estimated to be 95 and 96% at amino acid level for BnSHP1-like and BnSHP2-like genes, respectively. The phylogenetic reconstruction of SHP1 and SHP2 homologs from other species conglome...

Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3 

...chicken flocks. Sequence analysis revealed that the analyzed Egyptian isolates identified as very virulent Infectious bursal disease virus (vvIBDV). The identity between these Egyptian isolates and the vaccine strains is ranged from 89.5%-95.6% and 88.1%-97.8% -at the nucleotide and amino acid sequence levels, respectively. The IBDV-Egy1 isolate is related to the Egyptian vvIBDV strains but with some deviations in the amino acids (259V, 263F, 290I and 302N). T...

Kamran Aziz1*, Aamir Saleem1 and Arshad Mahmood Malik2 

...carried through chemical analysis in the laboratory and litter fall production was estimated by trapper technique. Under Cedrus deodara and Pinus wallichiana canopy, the leaf litter was collected and brought back to laboratory for the further chemical analysis. The Total annual litter fall production of Cedrus deodara was 4.2209t/ha/annum while the Pinus wallichiana was estimated 4.6097t/ha/annum. It showed that there is mor...
Farah Bilal*, Abdulmohsen Alhejaily, Shahida Husnain
...CLC). Somatic mutational analysis was done by QMC-PCR following direct sequencing with sangers’ method. Only one sample detected p.E545K mutation at c.1633G>A. Another most common mutation was observed in PIK3CA exon 20 which leads to change A>G at codon 1047. This transition converts amino acid histidine to arginine. Our study concluded p.H1047R most frequent while p.E545K rarely found mutations in PIK3CA gene in NSCLC population. Pr...

Shumaila Sadiq1,2*, Abdul Saboor2, Fouzia Jamshaid3, Abdul Qayyum Mohsin2 and Azeem Khalid

...climate change. In depth analysis of variables of sub-indices suggested that weather related hazards, coupled with extreme cold and hot months were much higher in Punjab. Drought impact was found higher in KPK and Balochistan followed by Sindh. Increasing share of small farmers and food insecure population also resulted in an increase in the sensitivity of farming community almost in all agro-climatic zones. Poor farm economy and lack of education were identif...

Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan 

...on protein. Phylogenetic analysis revealed that 20 samples are genotyped as very virulent NDVclass II of genotype VIIb, 4 samples were of high identity (94%-100%) with NDV class II of genotype II (vaccine strain) and 1 sample was phylogenetically related to NDV class II of genotype I with 98% identity. Furthermore, the intracerebral pathogenicity index (ICPI) for selected 5 virulent viruses reveals velogenic features with high pathogenicity index (1.60 to 1.74...
Lusha Liu1,*, Lei Cheng2, Xingqi Zhang3, Yulong Li1 and Jianping Jiang1,*
...n primers and do genetic analysis in the Sichuan basin population of M. fissipes. Of all, 35 primer pairs (57.38%) successfully amplificated in M. fissipes, of which 14 (40.00%) were polymorphism. The observed and expected heterozygosity in the test population ranged from 0.02 to 0.92 and from 0.02 to 0.62, respectively. High transferability rates were detected in M. butleri (37.14%), M. heymonsi (45.71%), M. pulchra (57.14%)...

Wesam Mady*, Nahed Yehia, Mohammed Hassan Elhussieny, Azhar Gaber Shalaby, Moataz Mohamed El Sayed, Neveen Rabie Bakry, Asmaa Shaaban, Abdel Satar Arafa, Afaf Abdel Baset Mahmoud and Wafaa Mohammed Hassan 

...3.4.4A, the phylogenetic analysis revealed that our sequenced viruses were clustered together in group B Russian like reassortant H5N8 viruses of clade 2.3.4.4.  

...
Muhammad Shahid1, Iram Amin1, Samia Afzal1,*, Zareen Fatima1 and Muhammad Idrees2

Ayub Khan* and Ghaffar Ali 

...666 per year. Regression analysis revealed that slope of the land, family size, income, land size, education, age and perception, were the important factors influencing WTP of the respondents in the study area. These findings suggests that sufficient funds can be generated from local farming community to implement the suggested program, and in this direction the concerned government and non-government organizations can play key role in educating and mobilizing...
Yan-Bin Zhu1, Wang-Dui Basang1, Zhan-Dui Pingcuo1, Yang-Ji Cidan1, Sang Luo1, Dun-Zhu Luosang1, Yang-La Dawa1 and Guang-Xin E2,3,* 
... components factor (PCA) analysis. These analyses inferred that the population cluster data was not only consistent with the populations’ geographic habits but could also be influenced by artificial selection and feeding style. Lastly, two credible genetic backgrounds were identified from the yak populations in this study using STRUCTURE software, which corresponded to previous knowledge about different molecular genetic markers. Therefore, unexpectedly,...
Salahud Din1,*, Saima Masood1, Hafsa Zaneb1, Habib Ur Rehman2, Saima Ashraf1, Imad Khan3, Muqader Shah4 and Syed Abdul Hadi1
...iometry and radiographic analysis of tarsal bones in twenty specimens of male and female adult chinkara (Gazella bennettii). The tarsus of chinkara comprises of five bones both grossly and radiographically, settled in three transverse rows viz, tibial and fibular tarsal in the proximal, central and fourth fused tarsal in the middle row, while, the first, second and third fused tarsal in the distal row. The fibular tarsal is the largest and longest bone ...

Asad Ali Khaskheli1*, Gulfam Ali Mughal1, Gul Bahar Khaskheli2, Allah Jurio Khaskheli3, Arshad Ali Khaskheli4, Abdul Samad Magsi5, Ghulam Shabir Barham2, Arab Khan Lund5 and Maqbool Ahmed Jamali4 

Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
... the FAdVs. Phylogenetic analysis revealed two distinct clusters among FAdVs. One cluster containing 3 strains belonged to the FAdV- C species and serotyped as FAdV-4 and showed close proximity at the nucleotide level. The other cluster containing three strains belonged to the FAdV-D species and serotyped as FAdV-11. Furthermore, the sequencing analysis of detected field strains revealed the high similarity and close cluster...
Muhammad Tariq Adnan Khan*, Tariq Mukhtar and Muhammad Saeed
... Brinjal PPL. Regression analysis showed positive and significant relationships between number of galls and reductions in shoot and root lengths and weights. As the plants of moderately resistant cultivar Brinjal Jamak suffered less damage and suppressed nematode infection considerably and therefore, recommended for cultivation in root-knot nematode infested fields to abate yield losses and repress the nematode from further multiplication. 
...

Muhammad Noman, Yong Tong Mu*, Muhammad Mohsin, Aamir Mahmood Memon and Muhammad Talib Kalhoro

...A (catch and effort data analysis) and ASPIC (a stock production model incorporating covariates) are used to analyze the fishery output (FO) and fishery input (FI) data of Scomberomorus spp. to evaluate their fishery stock status in Pakistani marine waters from Balochistan coastline.Three surplus production models (SPMs) viz. Fox (F-M), Schaefer (S-M) and Pella-Tomlinson (PT-M) along with three error assumptions (EAs), log (L-EA), log norm...
Muhammad Saeed1,2,*, Tariq Mukhtar1 and Malik Abdul Rehman3
...the year. The regression analysis between temperature and number of nematodes in the soil and females in the roots showed highly significant results at both the orchards. A direct relationship was observed between nematode population and temperature. Maximum nematode and female populations were observed at a temperature ranging between 26°C to 29°C at a soil depth of 30 cm. On the other hand, minimum populations were recorded at a temperature range of ...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *  
... decreased. SAS software analysis showed that there was no significant difference in H2S, CO2 and NH3 between the three groups (p>0.05), the same results showed that N, P, K, organic matter and ammonium nitrogen had no significant difference(p>0.05). These results suggested that the inoculation with Bacillus genus bacteria improved the microbial proliferation and shortened the time that the substrate ...
Jam Nazeer Ahmad1*, Taiba Sharif3, Samina J.N. Ahmad2, Sumaira Maqsood4 and Fariha Zafar3 
...ab, Pakistan through PCR analysis using mitochondrial cytochrome oxidase I (COI) gene based primers. Phylogenetic analysis was also complemented to differentiate fruit flies identified from other countries of the world. The sequencing results and phylogenetic analysis of collected specimens indicated that sequences of B. dorsalis, B. cucurbitae and B. zonata have 99-100% simi...
Joyce Osarogie Odigie*
...istical and biodiversity analysis with the extracted data for water quality analyzed via Weighted Arithmetic Index and pollution tolerance Index via pollution sensitive group. The dominant benthos group was Chironomidae: making up 20.99%, 18.47% and 16.65% of the benthic community. The results of WQI calculated across the three study stations were above the 100 benchmark. However, the PTI values recorded at the three study stations were less than 10, correlati...

Zara Ahmad1*, Fazli Rabbi2, Muhammad Zamin3, Bushra Kiran2, Tariq Shah2 and Shahzad Kouser4 

...antia) is considered for analysis. The primary data was collected from a sample of 110 farmers in the district of Faisal Abad, Punjab Pakistan. The data was analysed using Ordinary Least Square (OLS) technique. The results show that there is a significant difference between the on-season and off-season application of pesticides to bitter guard crop. The results clearly reveal that bitter guards’ yield is significantly higher in the off-season than on-sea...

Naveed Afsar* and Muhammad Idrees 

...d the overall regression analysis of the climate change perceptions reveal that climate change is happening and long-term mean temperatures have been increased with a significant decrease in the long-term mean rainfall over the past decades. The study recommends that farmers should be trained well to more appropriately tackle the menace of climate change, extension workers should be made sure to visit the farmers in their fields on regular basis, and Governmen...

Muhammad Arshad1, Muhammad Irfan Ullah1*, Muhammad Afzal1, Mian Anjum Murtaza2, Ejaz Ahraf3, Zahoor Hussain4, Syed Muhammad Ali Zahid1 and Maryam Riaz1 

... calculated by the image analysis method using Sigma Scan Pro 5.0 software. The results of the present study showed that CLM generated larger mines; 1.64 cm2 on Grapefruit, 1.44 cm2 on Kinnow and 1.40 cm2 on Succari compared to other five cultivars. However, the percent leaf damage due to the feeding of CLM larvae was observed higher 44.2% on Fairchild, 36.5% on seedless Kinnow, 36.3% on Feutrell’s early and 35.8% on Kinnow that showed great susceptibili...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1Muhammad Faheem Malik3 and Muhammad Idrees4

 

...e extensive phylogenetic analysis on the basis of partial helicase domain (ORF1) and capsid protein (ORF2) revealed clustering of Pakistani aHEV (Pak aHEV) strains with members of Orthohepevirus B species. However, Pak aHEV strains were highly divergent from other known members within Orthohepevirus B suggesting as novel aHEV strains circulating in the population of layer chickens in the country. Detection of HEV in layer chickens may pose...

Sajjad Ali1, Atta-ur-Rahman1 and Sher Ali2* 

...died for land use change analysis by comparing 1985 and 2015 Landsat images. Secondly, four sample union councils were targeted for medium level analysis. Thirdly, seven sample villages are targeted for micro level. The Landsat images of 1985 and 2015 are obtained from the open source Global Land Cover Facility (GLCF). The analysis revealed that the cultivated area was 146 square kilometer...

Muhammad Ilyas* and Fida Mohammad 

...le to carryout stability analysis for identifying high yielding RILs with suitable stability. 

...

Muhammad Zeeshan Manzoor1*, Ghulam Sarwar1, Mukkram Ali Tahir1, Noor-Us-Sabah1, Ayesha Zafar1 and Sher Muhammad2 

...re and post-harvest soil analysis was carried out for different physical and chemical characteristics. Sorghum biomass, plant height, plant diameter and no of plants/m2 were noted and it was observed from results that leaching fraction technique proved useful to mitigate the ill effects of brackish water. All the collected data were subjected to statistical analysis.  

...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...all 3D protein structure analysis of TNF-α gene. The association between polymorphism identified within the TNF-α gene with mastitis reported in this study revealed that SNPs has potential to serve as a molecular marker for screening of mastitis resistant and susceptible Sahiwal cows.  
...
Laiba Shafique1,*, Muhammad Afzal2, Syed Zakir Hussain Shah3, Mahroze Fatima4, Huma Naz5, Saif ur Rehman1, Youchuan Wei1 and Qingyou Liu1,*
...s of muscles for further analysis. Two-way analysis of variance under RCBD were applied on obtained data. Results demonstrated that growth performance (weight gain, specific growth rate and absolute weight gain) of C. idella fingerlings showed significant improvement by feeding formic acid and vitamin D3. Result showed that C. idella fed with vitamin D3 increased the crude protein, ash and...
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
... 2013-14. A phylogenetic analysis revealed that all the isolates belonged to sub-genotype VIIi with high similarity of 97.9 to 99.8% with similar viruses in this clade. Analysis of the hemagglutinin (HA) gene sequences of the two AIV was performed and phylogenetic analysis reveals genetically closely-related to H9N2 viruses classified into Mideast group-B and sub-lineage B2. The two strain...
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
.../i>and blood samples for analysis were taken from the subcutaneous abdominal vein. The experimental rations were almost identical in chemical composition. Results of nutrient digestibility and nutritive value, as total digestible nutrients (TDN) and digestible crude protein, showed that there were insignificant (P>0.05) differences between cows fed R2, R3 and R4 compared with those fed R1 (control), except digestibility of nitrogen free extract which w...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Naimat Ullah Khan3, Muhammad Asfandyar Khan2, Kashif Prince1,*, Mahboob Ali1, Ghazunfar Rashid1 and Azmat Ulah Khan2
...nventional PCR. Sequence analysis of 73 strains from diseased lambs showed 13.10% type A, 9.52% type B and highest 20.83% proportion of Clostridium perfringens type D. Out of 108 strains from kids, 54.62% were type A, 9.25% were type B and 36.11% were type D. Clostridium perfringens type C and E were neither detected in lambs nor in kids. The association of various factors (i.e. area, housing, seasons, colostrum feeding, hygienic condition...
Muhammad Raza Khan1, Tariq Mahmood1,*, Hira Fatima1, Faraz Akrim2, Shaista Andleeb1 and Abdul Hamid1
... in the study area. Scat analysis for diet composition showed 5 domestic and 8 wild prey species in its diet menu, with major share (53 %) from livestock and relatively less (46%) from wild prey. The grey wolf has emerged as one of the major predator in the study area consuming mainly donkey, horse, cow, sheep and goats, resulting in a negative perception among the locals. Most of the depredation occurs at night time while maximum livestock are depredated duri...
Muhammad Mansoor1, Khalid Usman2*, Shahid Hameed Khan Khalil3, Abdul Mateen Khattak4, Muhammad Ehsan Elahi1, Muhammad Waqas Imam Malik2 and Amir Hamza2
..., respectively. Economic analysis revealed that processing of fresh dates fetched more money than dry dates for most of the popular varieties. However, Dhakki date being more preferred due to its unique taste and the highest fruit yield and the biggest fruit size; needs curing under controlled conditions. 
...

Adewumi Moses Olubusuyi1, Ogunsakin Temitope Rachael1, Ogunrombi Sefiu Babatunde1, Ojeamiren Iduda1, Olawole Alaba Samuel1, Faleye Temitope Oluwasegun Cephas1,2* and Adeniji Johnson Adekunle1,3 

...uencing and phylogenetic analysis. Eleven and one of the 30 pools of RD and L20B cell culture supernatants, respectively, were positive for the RT-snPCR. Nine of the 11 amplicons from RD and the only from L20B were sequenced and identified as seven EV types; Coxsackievirus A4 (CV-A4), CV-A6, CV-A13, CV-A17 (both RD and L20B), CV-B2, Echovirus 9 (E9) and Enterovirus A76 (EV-A76). Our findings suggest some enteroviruses are present in and might be replicating in...
Shad Mahfuz1,2, Shuyuan Wang1, Mo Chen1, Fei Zao1, Dong Zhen1
Zhongjun Liu4 and Hui Song1,3*
...ere subjected to one-way analysis of variance using SPSS software followed by Duncan’s test probability of p<0.05. During the experimental period no significant differences were observed in egg production, egg mass, egg weight, feed intake and feed conversion ratio (FCR) among groups. Among the egg quality parameters haugh unit, egg shell color and egg yolk color were improved (p<0.05) in FVW fed groups than control and antibiotic gro...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
... and immunohistochemical analysis to detect this pathogen in pet dogs. Molecular methods, combining three types of PCR assays, identified two strains isolated from two pet dogs as Brucella canis. Histopathological changes revealed extensive inflammation and necrosis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the detection of B. canis...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...>P<0.05). Further analysis showed that CXCL8 and its mRNA had a significant correlation in the acute phase, and it was related to the severity of the disease and the course of the disease. The correlation between CXCL8 and its mRNA was significantly decreased in the recovery phase. There was only a weak correlation between serum CXCL8 level and serum Anti-MP level in both acute and convalescent stages (r= -0.2917, P=0.0891; r=-0.278...
Shagufta Naz1, Ayesha Javed1, Ayesha Saleem1, Khadija Murtaza1, Rukhama Haq1, Akbar Hayat2 and Neelma Munir1,*
...al weight loss, pyruvate analysis and sugar contents. Gamma radiation affected these parameters at higher doses but at 0.5 kGy no significant change was observed. 0.5 kGy prouting was inhibited and microflora was reduced at this dose without affecting its nutritional and sensory qualities. So, it was proved to be the best optimized dose was 0.5kGy.
...
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
...AGE and western blotting analysis showed that the secreted expression of rhP4H was achieved successfully in P. pastoris GS115 cells. The catalytic activity of rhP4H was tested in a system that contained O2, Fe2+, α-ketoglutaric acid and ascorbic acid. Liquid chromatography coupled with tandem mass spectrometry was used to monitor the hydroxylation of proline. The results showed that rhP4H specifically hydroxylated proline res...

Muhammad Zubair1, Sagheer Ahmad2*, Awais Rasool1, Muhammad Asad Farooq1 and Ibni Amin Khalil3
 

...ations. Hence GGE-biplot analysis tool can be applied facilitate evaluation of superior sugarcane varieties for different agro-ecologies. 

...
Azra1* and Shaukat Hussain2
...ive hierarchical cluster analysis with grain yield and disease severity categorized the tested genotypes into two major clusters. Cluster 1 is further divided into three sub clusters. In sub cluster I most of the genotypes are moderately resistant with medium to high yielding potential. Sub cluster II includes highly susceptible genotypes with low yield potential. Sub cluster III has moderately susceptible to moderately resistant genotypes. However, genotypes ...
Hande Kucukonder1, Pinar Celebi Demirarslan1*, Sezai Alkan2 and Özgür Baris Bırgul3
...ith nonlinear regression analysis and grey prediction model. For this, the growth of 118 broilers was analyzed by using their weekly individual live weights from hatch to 49 day-old. In the analysis, nonlinear functions and Rolling-Grey Model (1,1) prediction method were used. The time-dependent growths of mixed sexes broilers were analyzed in the aspects of testing the parallelism of female and male growth samples, determin...
Muhammad Qasim1*, Said Qasim2, Muhammad Naeem1, Amir Nawaz Khan3 and Shahid Iqbal4
...tagons were used for the analysis. Considerable decline in physical and economic assets were noted among all the three types of fishers, whereas the total value of livelihood assets for farming fishers deteriorated from 0.40 in 2001 to 0.33 in 2016. The study recommends that concerned authorities should take initiative to create awareness regarding conservation of fishes and observe strict implementation of the fishery laws and regulations.
...
Rakhshanda Kousar1, Tahira Sadaf1*, Muhammad Sohail Amjad Makhdum2, Muhammad Amjed Iqbal1 and Raza Ullah1
...e study in hand provides analysis of Pakistan’s comparative and competitive advantage in production of top three fruits, namely mango, citrus and dates by using Revealed Comparative Advantage, Revealed Symmetric Comparative Advantage, Relative Export Advantage Index and Log of Relative Export Advantage Index. For this purpose, time series data has been collected for the period 2001-2018 from Food and Agricultural Organization Statistics, International Tr...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...nt using log-dose probit analysis. Results revealed a mortality response of termites directly proportional to the extract concentration for all treatments. The floral extracts of basil (O. basilicum) and Tecoma (T. stans) exhibited maximum termite mortality (i.e. 55.5 and 50.0%, respectively) with minimum LC50 and LT50 values, followed by the extracts of chrysanthemum (G. segetum) and African marigold (T. erecta). Overall study results suggest that above menti...

Nosheen1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...rt>muscle. Regression analysis showed a positive relationship between POD activity and duration of exposure. It was also concluded that antioxidants enzymes can be used as a valuable biomarker of oxidative stress in aquatic animals. 

...

Aamir Saleem1, Arshad Mahmood Malik2*, Najam Ul Hassan1 and Imtiaz A. Qamar

...ng the physical-chemical analysis of the soil (ph, electrical conductivity, total nitrogen, organic matter and available phosphorus), crop parameter (Number of tillers, number of leaves, plant height, fresh land dry weight and crude protein) and meteorological data regarding rain fall, temperature, pan evaporation, sunshine and wind speed for interpreting results along with their statistical analysis as fixing legume with gr...

Wajid Shah Yousafzai1, Irfan Ullah1*, Amjad Ali1, Mahmood Iqbal2 and Salman Khan1 

Muhammad Azam Niazi1* and Umar Farooq2 

...ti rice. Supply response analysis was carried out to quantify the response of farmers to price and non-price factors. Most of the literature on supply response of crops in Pakistan has relied on the assumption that the data series used for analyses were stationary while most of time series are in fact trended. This makes the results of many earlier studies unreliable. This study uses the modern time series approach of cointegration using Autoregressive Distrib...

Arifa Khan1*, Shazia Erum2, Naveeda Riaz1, Abdul Ghafoor2 and Farhat Ali Khan3 

...evaluation and proximate analysis. Results showed significant differences in all yields and phenotypic quality traits. High sprouting (100%) and plant height was observed in CIP8 (31.60 cm). CIP12 produced more number of tubers/plant (23.4 tubers) with low tuber weight while better average tuber weight was observed in CIP22 followed by CIP13 genotypes (95%) while CIP7 showed minimum sprouting (70%).CIP28 genotypeproduced maximum plant height (70.00 cm) while m...

Muhammad Naeem Khan* and Asad Jan 

...etected. In quantitative analysis, among all the solvents, ethyl acetate fraction (EAF) had highest amount of phenol (27.4 mg/g), flavonoids (15.5 mg/g) and alkaloids (3.14 mg/g), while MCE displayed maximum amount of saponins (34.3 mg/g). In the proximate analysis, nitrogen freed extract was present in higher amount (38.45±0.83 %) followed by protein (30.26±0.72 %) while crude fibers were found least in amount...

Jahangir Khan*, Syed Attaullah Shah, Khurram Nawaz Saddozai, Muhammad Fayaz, Shahid Ali, Abbas Ullah Jan and Ghaffar Ali 

Jahangir Khan1*, Abbasullah Jan1, Kar Ho Lim2, Syed Attaullah Shah1*, Aditya R Khanal2 and Ghaffar Ali1 

... Results from regression analysis showed that age of the household head and his awareness of pesticides use over fruits, household size and their total monthly income were significant determinants of their WTP. In addition, health concern and environment concern of pesticides use also influence the WTP. Findings from this study help in assessing the market potential for pesticides-free fruits in Pakistan 

...

Tanveer Ahmad1*, Muhammad Mujtaba Rafiq1, Waqas Ahmed Dogar2, Abid Mahmood Alvi3, Qumer Iqbal4, Muhammad Azam5 and Arshad Ali Khan6 

...estimated by correlation analysis. Results indicated that phosphorus application @ 150g plant-1 has improved various traits i.e. number of fruit bush-1 (4866.7), yield bush-1 (2.19 kg), single fruit weight (0.656 g), number of fruiting nodes (12.18) and number of sprouted shoots cane-1 (13.89). However, vegetative characters particularly leaf area showed negative correlation (-0.264) with the increased amount of phosphorus. Fruit diameter (p=0.993) and length...

Raheel Saqib1*, Muhammad Luqman2, Iqbal Javed3, Abdur Rehman4, Muhammad Yaseen2, Saleem Ashraf5 and Muhammad Zeeshan Majeed2 

...s of multiple regression analysis showed that problem of poverty and hunger (PPH) can easily be estimated from different livelihood assets like education, ability to produce enough food, access to rural advisory (extension) services, access to financial support structures (in the form of social safety nets) and crop diversification strategies. 

...

Betül Ayça Dönmez, Sarbesh Das Dangol and Allah Bakhsh* 

...ng the histochemical GUS analysis. The highest gusA gene transfer efficiency rate both for leaf (60%) and internode (100%) explants was observed in cv. Desiree using Agrobacterium strain GV2260, while the highest gene transfer efficiency rate for leaf and internode explants in S. chacoense M6 were obtained with AGL1. The highest callus formation for both cv. Desiree and S. chacoense M6 was obtained on cv. Desiree leaf explants, transformed via the Agrobacteriu...
Muhammad Furqan Shahid1*, Tahir Yaqub1, Muhammad Yasin Tipu2, Asim Aslam2, Saima Yaqub1, Aziz-ul-Rahman3, Muzaffar Ali1
...istics. Sero-biochemical analysis of blood collected at 7th DPI revealed an increase in aspartate amino transaminase (246-308 U/L), alanine amino transferase (31-58 U/L) and blood urea nitrogen (11.21-22.58 mg/dl). 
...

Khaled Kaboudi* 

...poultry workers. Genetic analysis demonstrated that the most of H9N2 viruses circulating in North Africa were among the G1-lineage group. The presence of mammalian and human genetic markers were reported in several H9N2 strains, indicating possible risk for public health. Control of LPAI H9N2 subtype infection is currently based on biosecurity and the implementation of vaccination in some countries. Regional control strategy needs to be performed. Creation of ...

Muhammad Israr* and Noor Khan 

...nary Logistic regression analysis was conducted over the collected data-set to estimate a grower’s probability of adopting improved seed technology and quantify the role of FSC in adoption. Result confirms that membership in FSC was the most important determinant of improved wheat seed technology adoption. Head’s education level was the other important determinant with a positive significant effect on adoption of improved seed technology. The study...
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
...producers by qualitative analysis. Three isolates (MBL-SP02, MBL-CW01 and MBL-GN02) produced maximum zone of hydrolysis with maximum activity ratio. These isolates (MBL-SP02, MBL-CW01 and MBL-GN02) produced maximum units of mannanase activity, i.e., 9.474, 7.364 and 3.568 U/ml in quantitative, respectively.These best producers were further selected for optimization of cultural conditions for mannanase production. They all produced maximum mannanase unit...
Abu ul Hassan Faiz1,*, Fakhar-i-Abbas2, Mehboob ul Hassan3 and Lariab Zahra Faiz1
...study sites. The habitat analysis of study sites and correlation of species with habitat were done through Principal Component Analysis (PCA) by using software (XLSTAT-Ecology version). The habitat was positively correlated in the two areas while the study site Harighel fields was negatively correlated. It was also found that most species except for a few were positively correlated with habitat. The infestation of pest start...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...sequencing and molecular analysis of specific regions overlapping VP1-VP2 and VP2-VP3 genes of CAV strain from outbreaks in broiler flock (n=45) were undertaken in the Punjab Province of Pakistan. The phylogenetic analysis clustered study strains of CAV in group II similar to those reported previously from China. These findings are helpful for an understanding of currently circulating strains of CAV; however, they require fu...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...issected out for further analysis. Levofloxacin induced reduction in body weight while caused a noticeable increase in liver, kidney and testis weights. Significant increase in levels of total alkaline aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and bilirubin was observed in blood plasma. Urea level showed a remarkable decrease, whereas creatinine level increased but not significantly. Histological examinations exhibited py...
Peng Chen1,2, Zuhao Huang3,Chaoying Zhu1, Yuqing Han1, Zhifeng Xu1
Guanglong Sun1, Zhen Zhang1, Dongqin Zhao4, Gang Ge1 and Luzhang Ruan1*

Mahnoor Pervez and Farkhanda Manzoor* 

...ce liquid chromatography analysis. Pollen and nectar were collected from 2016 to 2017 from different agricultural areas of Pakistan during a field survey. Pollen was collected by installing traps and nectar was collected through capillary method allied with centrifugation. The HPLC technique was used for fast and simple detection of pesticide residues of commonly used pesticides (imidacloprid, deltamethrin, chlorpyrifos, fipronil, thiamethoxam, carbaryl, profe...

Turab Ali Kaurajo1*, Huma Rizwana1, Gulbahar Khaskheli2, Muhammad Haroon Baloch1, Muhammad Naeem Rajput1, Asad Ali Khaskheli3 and Mohsin Solangi

Imran1*, Amanullah1, Muhammad Arif1, Zahir Shah2 and Abdul Bari3 

...mpared with the economic analysis and profitability of soybean the highest net returns (NR) in Pakistani Rupees (PKRs) (PKR 62.082 ha-1) were noted with the biochar amendment followed by compost (PKR 60,168 ha-1), whereas least net return NR (PKR 41,548 ha-1) was recorded with peach residues incorporation. The value cost ratio (VCR) was highest with compost application (5.48) among the organic sources followed by biochar (5.37), while the least VCR value (4.67...

Sana Munir1, Muhammad Kamran Qureshi1*, Ahmad Naeem Shahzad2, Ismat Nawaz3, Muhammad Shahzad Anjam4, Sumaira Rasul4 and Muhammad Asif Zulfiqar5 

...ing various multivariate analysis such as principle component analysis and linkage cluster analysis. Principle component analysis (PCA) exhibited that first four PC out of nineteen PC, with eigen value >1, contributed about 80.326 % of total variability. Positive contribution towards PC1 was given by seed traits such as lint/seed, seed weight/seed, bo...
Javeria Zafar1, Asif Nadeem1*, Maryam Javed1, Fehmeeda Fatima1, Wasim Shehzad1, Ghulam Abbas1, Rajput Zahid Iqbal2 and Muhammad Muddassir Ali1
...versity and Phylogenetic analysis were carried out by the MUSCLE, DnaSP and MEGA6 tools. Total of 20 variations at different positions were found in aligned sequence results. Sequence conservation was observed for the ibex population. The overall results showed a close evolutionary relationship among Capra aegagrus, Capra nubiana, Capra falconeri, Capra hircus and Capra caucasica, demonstrating that they all were evolved from same ancestor. 
...
Barkat Ali1,2, Wasim Sajjad1,2,3, Imran Khan1, Muhammad Rafiq1, Sahib Zada1, Aamer Ali Shah1 and Fariha Hasan1,*
...sed on 16S rRNA sequence analysis. Antibacterial activity of the isolates was checked against ATCC strains E. coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Seven different types of antibiotic discs were used to check the intrinsic resistance of all the isolates to various antibiotics. Interestingly, most of the isolates were found resistant while only a few were susceptible, however, the isolates Lysinibacillus ...

Iffat Nawaz1*, Farhatullah1, Fida Muhammad1, Sajid Ali2 and Ghulam Muhammad Ali

... 49% accessions. Cluster analysis was performed and 96 accessions were grouped in four clusters (G1, G2, G3 and G4) based on primary seed characters. The G4 was further divided into two sub groups i.e., G4-A and G4-B. G4-A comprised 25 accessions whereas G4-B contained 30 accessions. The G3, G2 and G1 had 17, 16 and 8 accessions, respectively. Considerable variation was found among all the groups. Conservation and characterization of this diverse germplasm is ...

Syeda Naila, Muhammad Ibrar, Fazal Hadi* and Muhammad Nauman Khan 

...der drug microscopy, ash analysis, extractive values and fluorescence analysis were also studied. Preliminary phytochemical screenings of different extracts of Oxalis pes-caprae L. showed the presence of carbohydrates, phenols, flavonoids, tannins, saponin, fats and oils 

...

Sanaullah1, Fazli Rabbi2*, Shahid Ali2, Zeeshan Khan3 and Muhammad Zamin4 

...iques were used for data analysis. The results reveal that 98 percent of the sampled tourists were willing to pay for the development and conservation in the area. Moreover, 37 percent of the sampled tourists were willing to pay Rs.100 (0.94 US$) per visit as an entry fee. The analysis further showed that an estimated amount of 421 million Rupees (3.97 million US$) could be generated as consumer surplus. The poisson regressi...
Zübeyde Kumbıçak1,* and Hatice Poyraz2
...e describe a chromosomal analysis of Drassodes bifidus and Drassodes serratichelis using a classical Giemsa staining method. We collected specimens from different populations in Nevşehir and used totally fourteen male spiders. The two Drassodes species had karyotypes comprising 10 pairs of autosomes plus sex chromosomes which were X1X20 (♂) type. All chromosomes including sex chromosomes were telocentric and relati...
Sumaira Abbas1, Muhammad Sultan Haider2,*, Fatima Kafayet1, Sana Ashraf2, Atifa Masood3 and Moazma Batool4
...us skin. Statistical analysis of results showed non-significant differences (P<0.05) among all treatments in weight gain and FCR. Maximum color intensity 1.52±0.08 and carotene concentration in lateral and tail region, 0.4±0.01 and 0.05 ±0.02 were recorded in T3 without any harmful effect. It is concluded that citrus peels are good, natural, low price Carotene source for color enhancement.
...
Saman Muhsin Abdulkareem* and Nadir Mustafa Nanakali
... removed for biochemical analysis including the level of oxidative stress biomarkers, liver enzymes, and the expression of CYP1A1 gene. The results of this study indicated that exposure to TCDD (10µg/kg/day) could significantly increase liver oxidative stress biomarkers and serum liver enzymes (p<0.05). While, pre and post treatment of QCT (20 mg/kg/day) had protective effects on hepatotoxicity induced by TCDD and could significantly decrease t...
Zekeriya Demir1 and Hatice Kaya2*
...n, according to economic analysis, the net income for hen fed 1.5% BP addition dietincreased by about 0.62% compared to control group. Therefore, bee pollen may be used in laying hen diets because of its psitive effects on FCR, serum cholesterol and lipid contents.

 

...

Noorullah Khan1*, Farrukh Siyar Hamid1, Fayaz Ahmad1, Sabaz Ali Khan2, Imtiaz Ahmed1, Muhammad Abbas Khan1, Shamsul Islam1, Abdul Waheed1, Basharat Hussain Shah1 and Hussain Shah

...of 5000 ppm. Correlation analysis revealed that SSR % and growth of kiwi seedlings are strongly associated with NFARP as we found strong correlation (P<0.001) among NFARP, SSR% and plant height. In addition, the main root length also showed moderate correlation (P<0.05) with plant height but it was not correlated with SSR%. Based on this finding, it is concluded that IBA concentration of 3000 ppm is optimum for better seedlings growth and evaluation of s...
Huanxin Zhang1,2, Hongshuo Tang3, Jun Chen1, Yu Zang1,2, Xuexi Tang1,2,* and Ying Wang1,2,*
Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8
...ccording to phylogenetic analysis, 5 IBDV strains showed characteristic amino acid signatures in the VP2 gene (A222, I242, I256, I294, S299) and classified as vvIBDV. Furthermore, the sequencing analysis of detected field strains revealed the high similarity and close clustering with vvIBDV strains isolated from neighboring countries, suggesting geographic and temporal relationships among t...
Bibi Nazia Murtaza1, Mazhar Saeed Chaudry2, Shamaila Inayat Nadeem1, Muhammad Shahid Nadeem3 and Abdul Rauf Shakoori4,*
...ime in NHL. By in silico analysis, it is anticipated to be a diseases causing or pathogenic alteration by Mutation taster and SIFT analysis. Spotting the rs112445441 in NHL is supporting the idea of cross talk between RAS-ERK-MAPK and PI13K and this could be one of the factors behind the development of resistance to the current therapy and relapse in NHL. K ras mutant isoforms, being the negative predictors for prognosis, ar...
Kadry A. El-Bakry1, Lamiaa E.M. Deef1*, Lotfy Z. Habbak1 and Samia S. El-Naeli2
... samples for biochemical analysis and liver tissues were collected and fixed in 10% formalin for histopathological studies. The present study showed that, PAT causes elevated alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and significantly increases serum level of malondialdehyde (MDA) but decrease the activity of superoxide dismutase (SOD) enzyme. On the other hand, in patuilin injected rats and treated with ginger, the activit...
Muhammad Muneeb1, Muhammad Ayub1, Sher Bahadar Khan2,*, Amjad Sohail1, Farkhanda Jabeen1, Mumtaz Ali Khan3, Amjad Khan4, Kashif Prince3, Asghar Khan5 and Irshad Ahmad6
...i. In physiochemical analysis high pH was observed in samples taken from Warsak Road (6.84) followed by Hashtnagri. High moisture content was observed in samples taken from Saddar Bazaar (51.70%) followed by Tehkal Region (50.94%) while the high Ash content was found in Charsadda Road (1.81%) samples followed by Hashtnagri (1.76%). The highest fat content was observed in samples taken from Chowk Yaadgar (17.92%) and maximum protein content was found in sam...
Erum Iqbal*, Nasir Mehmood, Nasira Kazi and Shahina Fayyaz
...encountered. Soil sample analysis revealed two and a known plant parasitic nematode species viz., Paratylenchus manilkarii n. sp., P. sindhicus n. sp.,and Pratylenchus kralli Ryss, 1982 as a new record species. P. manilkarii n. sp., is characterized by the lateral field with four incisures; stylet 28-29 µm long; vulval lips protruding with vulval flap; lip region rounded or truncated without submedian lobes, tail ventrally cur...
Sajida Rasool1, Saba Irshad1*, Neelam Saba1, Mehak Fiaz1Muhammad Sajid Hussain2, MuhammadWajid Hussain3 and Peter Nürnberg2

 

...res. Genome wide linkage analysis and whole exome sequencing revealed a novel homozygous nonsense mutation (c.204T>G) in BICD2 gene which was predicted to yield a truncated protein product (p. Glu68*). This is first nonsense mutation being reported causing HSP with complex clinical features and early onset. Further, functional exploration will be required for genotype phenotype correlation.
...

Muhammad Sohail Memon1*, Khadim Ullah1, Altaf Ali Siyal1,2, Naimatullah Leghari1, Ahmed Ali Tagar1, Khalil Ahmed Ibupoto1, Syed Tahir Ata-ul-karim3, Muhammad Tahir4 and Noreena Memon1 

...m depth desired depth to analysis the Physical chemical properties before and after the trial. Irrigation water was measured through cutthroat flume at each irrigation interval and the total water consumed by ridge ground was 500 mm. The solute transport and soil water content in the root zone was also simulated by HYDRUS-2D. The results reveal that the salts moved upward to the top (upper layers) and concentrated at the center of the ridge for treatments T2 a...

Naglaa M. Hagag1*, Mervat E. Hamdy1, Mary A. Sargious1, Sara M. Elnomrosy1, Nahed A. Ahmed1, Ayman A. Hamed1, Ahmed R. Habashi1, Essam I. Ibrahiem1, Mahmoud A. Abdel-Hakim2 and Momtaz A. Shahein

...e detected. Phylogenetic analysis of the study sequences indicates that the circulating FMDV serotype SAT2 viruses were homogeneous and related to Topotype VII. Importantly, the newly emerged viruses were closely related to strains isolated from Libya in 2012 (Topotype VII, lineage 3), with 92–93% amino acid identity, and were clearly separated from SAT2/GVII/Gharbia/Egy/2012 and SAT2/GVII/Alex/Egy/2012 (Topotype VII, lineage 2), indicating a new introdu...
Nurlybay Kazhgaliyev1*, Talgat Kulmagambetov2, Dulat Ibrayev1, Saule Bostanova1 and Zhanat Titanov1
...gus herd-mates. Chemical analysis of feedstuff was carried out in Altyndan LLP and AKA whereas physiological and clinical indicators of second-generation heifer calves were within the normal range in various seasons. The highest thermal resistance index was in animals of group I and II groups (76.4 and 78.0, respectively). Under the conditions of Akmola and North-Kazakhstan regions, the thermoneutrality or comfort temperature zone for Hereford and Aberdeen Ang...
Yan-Guo Han, Yu-Qing Han, Jia-Yuan Wu, Jia Guo, Ri-Su Na, Yan Zeng, Guang-Xin E, Yong-Ju Zhao and Yong-Fu Huang*
...
This study aimed to analysis the effect of immunisation of somatostatin (SS) DNA vaccine in maternal animals on serum related hormones in offspring. In this study, ten ewes with two newborn lambs were randomly divided into SS DNA vaccine group (Group T) and empty vector group (Group C), and these vaccines were given orally into the ewes at weeks 0, 3 and 6 of the research. Blood samples of ewes or offspring lambs were collected at week 6 or 9 of lactation...

Muhammad Bakhtiar Khan1 and Jangraiz Khan2* 

...earch study the economic analysis of Tunnel farming of off-season vegetables has been done for district Peshawar. Primary data was collected from eighty-four farmers who had grown off-season vegetables through tunnel-farming, and seasonal vegetables without tunnel and their productivity were compared. It was found that output per acre in tunnel was significantly higher than that of without tunnel. All the three vegetables viz tomato, cucumber and bottle gourd ...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...tor proteins. Sequencing analysis of the black, brown and white different color MC1R genes of Nubian goats revealed that there are three SNPs in the gene sequence, which are 219, 712 and 1160, respectively. The C/T mutation did not cause amino acid mutations; base A/G mutations occurred at position 712 and caused amino acid mutations in pure black and brown goat samples, which were mutated from tryptophan to cysteine. The results of this study have important r...

Kalpana Polisetty1* and Kiran Kumar Paidipati2 

...e change point and trend analysis for wheat production on the basis of time and spatial scale of major wheat producing states of India. This study has considered various prominent non-parametric methods i.e. Pettitt’s, Standard normal homogeneity (SNH) and Buishand’s range tests. The core purpose of these three techniques is to capture the mutation point in time series data connected to wheat production during the period 1980-2016. Consequently, Se...

Asif Yaseen1, Muhammad Israr2*, Allah Bux Khan1, Ahsan Javed3, Nafees Ahmad4 and Zia Ullah

Saleem Ashraf1, Raheel Saqib2*, Zakaria Yousuf Hassan3, Muhammad Luqman4 and Abdur Rehman5  

...respondents. Correlation analysis unveiled that most of constraints impeding growers’ benefits were inter-correlated. This implies that resolving one barrier could limit another. This study urges a need to regulate middleman in marketing system through legislation and government should develop systematic marketing channels and support to develop fruits industry or value addition. 

...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
Tanveer Ahmad1, Tariq Mehmood1, Muhammad Shahid1, Adnan Ahmad1,2, Muhammad Asim Raza Basra1, Muhammad Zia-ur-Rehman3* and Munawar Ali Munawar1
...e test (OGTT) percentage analysis on Sprague Dawley (SD) rats at the dose of 20mg/kg using glibenclamide (GC). Among these synthesized pharmacophores, six compounds exhibited percentage reduction (20.47±2.54 to 44.97±2.16 %) and (20.79±1.55 to 37±2.94 %) in blood glucose level at 20mg/kg dose compared to glibenclamide (74±3.10 % reduction) 50mg/kg dose of glibenclamide after 2 and 5 hours of oral administration respectively. ...
Muhammad Asif Gondal, Qazal Waheed, Sana Tariq, Waseem Haider, Aisha Khan, Qudsia Rasib and Haroon Ahmed*
...of ecology, phylogenetic analysis, spatio-temporal variation and diversity.
...
Chuntao Li1, Hua Liu2, Jianqing Zhang1*, Jiaqiang Zhang1, Luming Dai1, Zhihuan Zhao1,Lizhou Fang1, Ling Liu1, Jingkui Shu1 and Jiagang Feng1
...tory by human microarray analysis. Then, we selected lncRNAs and their associated mRNAs which were both differential expression in COPD vs healthy smokers, for quantitative reverse transcription PCR (qRT-PCR). A total of 3,359 lncRNAs revealed by microarray analysishave been differentially expressed between the two groups, 1,995 lncRNAs identified to be up-regulated in COPD, whereas 1,364 lncRNAs have been down-regulated. Me...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...6 isolates. Phylogenetic analysis of the tet (O) gene sequences recovered from the C. jejuni isolates revealed that the strains isolated from chickens and drinking water from the same farm were identical. However, the sequence ofthe tet (O) gene from human isolates was highly similar to that from drinking water isolates. Our findings highlight the presence of MDR Campylobacter strains in chickens and the role of drinking water as a ...
Bhandari Jyoti1,2, Yasen Shali3, Zhang Zehua4, Yao Xiaoho5, Deng Jiang3, Zhang Yaling5, Wang Cuiling5, Li Yang5, Lei Xueping5, Wang Wenfeng5, Mohammad Farooque Hassan6 and Li Can1,*
...’s M*-m regression analysis, Taylor’s power law and aggregation cause analysis showed an aggregated distribution pattern for L. m. tibetensis Chen. It is concluded that insect behavior and environmental conditions co-influence the population density and spatial distribution pattern of this pest.
...

Dilawar Khan1*, Arif Ullah2, Zainab Bibi1, Ihsan Ullah1, Muhammad Zulfiqar4 and Zafir Ullah Khan

Malak Atiq Ullah Khan1*, Fida Mohammad2, Fahim Ullah Khan3, Sheraz Ahmad2, Mian Ahmad Raza1 and Tariq Kamal

...ction (AMMI), GGE biplot analysis and stability parameters. Eighty-one wheat genotypes were evaluated during three consecutive years (2013/14, 2014/15 and 2015/16) across nine environments (sites × year combination) in Khyber Pakhtunkhwa, Pakistan. Graphical stability approaches such as AMMI and GGE provided almost similar results for identifying the high-yielding and stable wheat genotypes. The AMMI analysis identifie...

Mohammad Aquil Siddiqui1*, Muhammad Tahir Khan1, Ghulam Shah Nizamani1, Shafquat Yasmeen1, Imtiaz Ahmed Khan1, Abdullah Khatri1 and Nighat Seema Soomro2 

...ting traits. Correlation analysis of the agronomic parameters implied that number of branches plant-1, seeds pod-1 and 1000 seed weight had highly significant correlation with the seed yield of the lentil. It was concluded that the mutants AEL-40/30 and AEL-12/30 have very high yield potential, and these genotypes can be further evaluated for zonal performance in the Sindh province.  

...
Nadeem Munawar1,2*, Tariq Mahmood2, Paula Rivadeneira1, Ali Akhter2 and Saqib Mehmood2
...er 14 months, vegetation analysis was conducted using the quadrate method to record the vegetation around rodents’ active burrows at field boundaries. The dominant wild vegetation included Cynodon dactylon, Saccharum griffithii, Dactyloctenium aegyptium, Dichanthium annulatum, Desmostachya bipinnata, Imperata cylindrical, Ziziphus nummularia, Achyranthes aspera, Calotropis procera, Sorghum halepense and Capparis deciduas. This...

Muhammad Ilyas1, Sardar Ali Khan1, Shahid Iqbal Awan1, Shafiq-ur-Rehman1, Waqar Ahmed1, Muhammad Riaz Khan2, Raja Mohib Muazzam Naz2, Muhammad Mohib Ullah Khan3 and Sumaira Hafeez1* 

...ustify;">Generation mean analysis belongs to the quantitative biometric methods based on measurement of phenotypic performances of certain quantitative traits on as many as possible plant individuals in basic experimental generations. Initially, 108 inbred lines of maize were planted in two separate trails under water deficit and normal irrigation conditions in the experimental field of Department of Plant Breeding and Molecular Genetics. Selection of parents ...
Chen Shi, Jianlong Zhang and Guanghui Teng*
...ctively. The statistical analysis of the body ratios could be used for dividing the different growth stages that replaces the body weight or growth day method. It also can provide a scientific foundation for precision feeding and automatic management of individual pig that could be applied in the real farm.
...

Mudassar Rashid1, Zuhair Husnain2, Usman Shakoor1* and Muhammad Iftikhar ul Husnain

... area) had been used for analysis. Historical climate data from 1981-2015, was collected from Pakistan Meteorological Department, while data of Technology, fertilizer and cotton area has been gathered from Pakistan Economic Survey. ARDL bound testing econometric approach was employed to gauge the empirical estimates. The outcomes of cointegration shows that all climate variables have statistically substantial effect on cotton productivity in Pakistan. Both rai...

Sanaullah1*, Urooba Pervaiz1, Shahid Ali2, Mohammad Fayaz2 and Aftab Khan

Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...(9.0-9.5). The taxonomic analysis revealed that these isolates were closely affiliated with the genus Streptomyces (62.5%), Lentzae (6.25%), Pseudonocardia (6.25%), Lechevalieria (6.25%) and Nocardiopsis (12.5%). Most of the isolated strains exhibited strong inhibition against a broad range of pathogenic test organisms. The partial purification and metabolite profiling using ultra performance liquid chomatography and mass spe...
Muhammad Irfan Ullah1*, Muhammad Arshad1, Sajjad Ali2, Asad Abdullah1, Samina Khalid3, Hafiz Muhammad Aatif4, Syed Muhammad Ali Zahid1, Muhammad Afzal1 and Jaima Molina-Ochoa5
...using BioLeaf for foliar analysis. Our results demonstrated that the quantification of foliar damage using BioLeaf application was easy and accurate. This method is also comparable with other techniques like digital analysis by ImageJ software and leaf area meter devices. With these results, it is concluded that the BioLeaf application has resulted in an accurate leaf area measuring tool of foliar damage with minimum time an...
Jun Yan Bai*, Hong Deng Fan, You Bing Yang, Xu Wang, Heng Cao, Xue Yan Fu and Yu Qin Wang
Jun Yan Bai*, Xiao Hong Wu, Shuai Yang, You Zhi Pang, Heng Cao, Hong Deng Fan, Xue Yan Fu, Kun Peng Shi and Xiao Ning Lu
Jun Yan Bai1*, Xiao Ping Jia2, Xiao Hong Wu1, Guang Lu Li1, Heng Cao1, Xue Yan Fu1 and Kun Peng Shi1
... According to clustering analysis, Beijing white quail has the closest genetic relationship with Japan quail, partridge, and mallard, which are clustered in one group.
...
Mubashra Salim1, Omeira Ibrahim1, Hugo Vilhena2,3,4, Carla Maia5, André Pereira5, Maria Shahzeen1, Shabana Kalsoom1, Asim K. Mahmood6 and Furhan Iqbal1*
...uencing and phylogenetic analysis was performed in M. haemofelis infected samples. Analysis of risk factors revealed that risk of M. haemofelis and of C. Mycoplasma haemominutum infection was significantly higher during summer months than during the winter season (P ≤ 0.01 in both agents). Cats older than one year of age were significantly more predisposed to C. Mycoplasma haemominutum infectio...
Ahsan Numan1,4, Khadija Irfan Khawaja2, Abdul Basit Qureshi3Muhammad Shahbaz Yousaf4, Imtiaz Rabbani4, Hafsa Zaneb5 and Habib Rehman4*
...iple logistic regression analysis showed that the DSP was significantly associated with age of the patients (OR:1.40, CI:1.24-1.67), duration of DMT2 (OR:1.24, CI: 1.01-1.35), HbA1c (OR:1.86, CI:1.61-2.05), blood fastening sugar level (OR:1.37, CI: 1.18-1.51) and triglyceride concentrations (OR:1.13, CI: 1.02-1.33). In conclusion, the DSP was considerably higher in DMT2 patients and was significantly associated with age of patients, duration of DMT2...
Saeed Murtaza1, Abdul Sattar1*, Nasim Ahmad1, Muhammad Ijaz2Maqsood Akhtar3 and Muhammad Shahzad4
...s while milk composition analysis was done once a week. Results depicted that initially, anechoic lumen filled areas with echogenic border in cervix and uterus was found but at involution, cervix and uterus became moderately hyperechoic without any fluid filled areas. Moreover, there was non-significant effect (P>0.05) of treatment on cervix, uterine body, non-gravid and gravid horn at involution among three groups when these attained non pregnant size. Reg...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...nd kidneys were used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF group and the control group with regard to heart rate, blood pressure, serum creatinine, BUN, LDH, cytokines and urinary electrolyte levels. Furthermore, monocyte and neutrophil apoptosis, tissue luminol, lucigenin, malondialdehyde and collagen levels were found ...
Zhi Yi Jin and Shao Ming Qin*
...ots. Principal component analysis showed that safety factors (vegetation cover on both banks, distance to a mine factory and river width) were the three most important habitat factors, indicating that Scaly-sided Merganser had high requirements for shelter and safety. Twenty two species of fish belonging to eight families and three orders were identified in foraging areas of the Scaly-sided Merganser. We could identify only six fish species eaten by the mergan...
Asim Faraz*
...subjected to statistical analysis with 2×2 factorial arrangements of treatments under completely randomized design. After 120 days trial period the mean body weight and average daily gain (ADG) of male and female calves was significantly increased (P<0.05) in IMS as 80.8±2.7, 77.8±2.7 kg and 0.67±0.02, 0.65±0.02 kg/d than EMS as 64.7±2.6, 52.3±2.6 kg and 0.54±0.02, 0.44±0.02 kg/d of male and fema...

Saima Sharif*, Farkhanda Manzoor, Tasnim Farasat, Shaugfta Naz and Raheela Tabasum 

.../min/1.73m2. Statistical analysis was donedone by using SPSS. The serum creatinine of ischemic stroke patients was found to be 2.41 mg/dl compared to 0.93mg/dl in controls. The blood urea in ischemic stroke group was 67.3 mg/dl in contrast to 34.6 mg/dl in controls. The eGFR in ischemic stroke patients was calculated to be 54.62ml/min/1.73m2, compared to 85.90 ml/min/1.73m2 for controls. Prevalence of eGFR <60 ml/min/1.73m2 in patients with stroke was 63%, ...

Muhammad Asif Farooq1*, Muhammad Jalal Arif1, Muhammad Dildar Gogi1, Bilal Atta2 and Ahmad Nawaz

... subjected to analytical analysis using QuECHERS for sample preparation followed by quantification with GC-ECD. Recoveries of the samples analyzed were ranged from 78-98%. Results revealed that 75.00% samples of pesticide residues mitigation in PRMM-4 followed by PRMM-3 (66.66%), PRMM-2 (33.33%) and PRMM-1 (25.00%). Similarly, the highest number of samples (41.66%) from PRMM-4 exceeded maximum residual limit (MRL) values of the Codex Alimentarius Commission wh...
Bo Gao1,2, Fengmei Yang3, Wei Chen2, Xiaojia Song4, Xiaobo Liu5 and Dongmin Li1*
...aracteristic (ROC) curve analysis, their diagnostic threshold values were calculated. MDR1 expression levels were the highest in malignant tumor tissues, followed by adjacent tissues, and the lowest in non-cancerous tissues. Differences in expression level between varying degrees of differentiation and/or lymphatic metastasis, as well as variations in negative and positive expression between survival and recurrence times, were statistically significant. The le...
Peimin Yang*, Zongyun Hu, Yixin Liu, Guanghai Jin and Lei Wang
...five locations. Sequence analysis showed that there were 50 polymorphic sites and 20 haplotypes among five populations, and the samples exhibited high haplotype diversity (h=0.8467) and low nucleotide diversity (Pi=0.0063). Genetic distances ranged from 0.0002 to 0.0149 within and 0.0007 to 0.0140 between populations. AMOVA analysis indicated significant genetic differentiation among populations (...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*
...udy was immunophenotypic analysis of mesenchymal stem cells from dog adipose tissue during in vitro cultivation. Materials and Methods: Mesenchymal stem cells were obtained from dog adipose tissue by explant method. The cells were cultured in CO2 incubator by standard procedure. Expression of cytoplasmic and membrane proteins on dog stem cells from fat tissue at the IVth and Xth passages was examined by immunohistochemical meth...

Arjumand Nizami1, Muhammad Zulfiqar2*, Jawad Ali1, Naushad Khan2 and Imran Sheikh

...yzed using MS-Excel. The analysis of the data reflected that although canal water was available but about 80% at head, 50% at middle and 20% at tail. Thus remaining irrigation water requirement i.e. about 20% at head, 50% at middle and 80% at tail was met through tube well operation. The result from before and after AWD technology and laser land leveling technology adaption shows that 24.24% tube well operation time was reduced at the head, 19.4% reduced at mi...

Bahadar Sher Khattak1, Muhammad Kaleem1, Waqas2*, Humaira Naz1 and Muhammad Yasir

...terviews. After the data analysis, it was found that the majority of the respondents (83.3%) who were small farmers acquired and utilized micro-credit in establishing income generating entrepreneurs, purchased quality seeds, fertilizers and small scale modern technology. These innovations brought substantial enhancement in their farm productivity and income that ultimately improved those farmers’ socioeconomic position. However, 16.7% of the respondents&...
Gong Ga1,2 and SuoLang Sizhu2,*
... by immunohistochemistry analysis. Overall, we found that that eight serum samples (4.6%, 8/173) were positive to anti-HEV IgM antibody and Seventeen serum sample (9.8%, 17/173) were positive for anti-HEV IgG antibody. The HEV RNA positive rate was of 4.6% (8/173) in feces and serum. Moreover, the prevalence of HEV RNA was 9.5% (2/21) in swine liver, 4.8% (1/21) in swine spleen, 9.5% (2/21) in swine kidney, 4.8% (1/21) in swine intestine and 14.3% (3...

Muhammad1*, Muhammad Suleman Bacha2 and Syed Akhtar Ali Shah1 

...udy performs a focalized analysis and investigates the influencing factors of urban farmers’ Willingness to Pay (WTP) for organic fertilizer in District Mardan, Pakistan. The Contingent Valuation Method (CVM) was used and data were randomly collected from 384 heads of the household of urban farmers. Data was set in SPSS software version 22.0 and analyzed using descriptive statistics and Logit Model. The study revealed that a major proportion (62.5%) of s...

Noor-us-Sabah1*, Mukkram Ali Tahir1, Ghulam Sarwar1 and Sher Muhammad

...nt study. After chemical analysis, different organic amendments were used in the study. Following treatments were applied in three replicates i.e. T1- control (recommended NPK), T2- ½ of recommended NPK, T3- full dose of FYM, T4- full dose of press mud, T5- full dose of compost, T6- full dose of poultry manure, T7- full dose of sesbania green manure, T8- T2 + ½ FYM, T9- T2 + ½ press mud, T10- T2 + ½ compost, T11- T2 + ½ poult...

Fazli Ahad1, Raziuddin1, Nazir Ahmad1,2*, Muhammad Nauman1, Touheed Iqbal3, Nabeel Khan1, Fazli Hameed4 and Quaid Hussain2 

...hese traits. Correlation analysis indicated that flowering (rp=0.45), primary branches plant-1 (rp=0.49), main raceme length (rp=0.41), seeds pod-1 (rp=0.41) and 1000-seed weight (rp=0.38) had positive and significant association with seed yield plant-1. Genotypes, DH4, DH5, DH8, CA5 × DH6, CA5 × DH7, CA5 × DH3, and CA2 × DH7 displayed well genetic potential for the majority of the traits hence could be used in future rapeseed breeding ...

Zulfiqar Ali Abbasi1*, Muhammad Nawaz Kandhro1, Aijaz Ahmed Soomro1, Naimatullah Leghari2, Muhammad Ibrahim Keerio3, Ahmed Naqi Shah1 and Musrat Begum Abro1 

Nadia Mangrio1*, Muhammad Nawaz Kandhro1, Aijaz Ahmed Soomro1, Nihaluddin Mari3 and Zia-ul-Hassan Shah2

... 10H2O). The statistical analysis of pooled data of two years experiments indicated that growth, yield, quality and plant nutrients contents of sugarcane were affected appreciably (P<0.05) by Zn and B as judged to check plots. Zn at 15 kg ha-1 (soil applied) resulted in enhanced traits particularly cane yield (111.9 t ha-1), brix (23.1%), and plant nutrients content such as N (2.4 %), K (0.7 %), Zn (68.5 ug g-1) and B (31.7 ug g-1). Similarly, foliar applic...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1  
...family. The phylogenetic analysis also showed the genetic positioning of A. chukar with respect to other different reported species as well. The study gave us useful genomic information about genetic diversity in A. chukar and its phylogenetic relationships with related taxa, emphasizing on need of execution of conservation strategies to protect this unique genetic resource of Pakistan.
...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
...rect sequenced. Sequence analysis showed 94% similarity to Stenotrophomonas maltophilia which is considered to be the second most prevalent bacterial species in mosquitoe’s midgut. This observation may lead  towards the confusion that the outbreak is either of Dengue or Chikunguna virus. In conclusion, confirmatory molecular characterization of the viral genome remains controversial and further studies are needed in this respect.
...
Weihao Chen1,2, Zhilong Tian2, Lin Ma2, Shangquan Gan3, Wei Sun1,4,* and Mingxing Chu2,*

Amjad Ali1*, Lal Almas2, Syed Attaullah Shah1, Hina Fatima3 and Asim Khan1  

... Results from efficiency analysis show that age and off-farm income are quite influential socio-economic factors that affect technical efficiency of sugarcane growers in the study area. On inputs side farm size, labor, seed rate, tractor hours, irrigation and urea were found statistically significant. Therefore, based on findings, it is recommended that in order to encourage growers to apply optimal amount of these inputs and avoid uncertainty in returns, an e...
Qingzhan Ma1, Bo Wu1, Jianping Jiang2 and Zhaobin Song1,3*
...re, population structure analysis and UPGMA dendrogram suggested that there were close genetic relationships between POPA and POPE, POPA and POPG, and POPF and POPH, respectively. POPH showed serious inbreeding based on Fis analysis.
...

Saira Bano, Muhammad Naeem* and Samrah Masud 

...th (PDW) For statistical analysis, regression was applied to the data. Fish fed with 25% dietary protein level under T3 showed good health status as compared to T1 and T2 with 15% and 20% dietary protein levels. Results of the research work indicated that L. calbasu reared by feeding 25%CP showed better health status.  

...

Rabia Iqbal, Muhammad Naeem*, Samrah Masud and Abir Ishtiaq 

...al) for body composition analysis. Mean percent water, ash, fat and protein contents were ranged 80.55±0.94 to 80.55±0.94; 2.23±0.13 to 2.23±0.13, 5.85±0.55 to 6.63±0.97 and 10.59±1.67 to 11.93±1.67 in wet body weight of hybrid fish (Labeo rohita ♀ and Catla catla ♂), respectively, reared under different treatments. Mean water and fat contents showed highest percent values in T1 (15%CP), while ash and...

Akbar Hayat*, Muhammad Nawaz Khan, Ehsan Ul Haque, Ahmed Raza, Faheem Khadeeja 

...in 2013. The statistical analysis of vegetative data from trial site shown good compatibility in term of scion/root stock ratio. Maximum plant height attained in T0(Rough lemon) with max. canopy volume. All the exotic root stocks are compatible with Feutrell’s early. Objectively maximum fruit weight, fruit size, juice percentage and yield obtained in T1 Troyer citrange and T2 Cox mandarin as compared to local root stock rough lemon. Results depict that e...

Gulnaz Hameed1, Abdul Saboor1, Khuram Nawaz Sadozai2*, Ghaffar Ali2, Dawood Jan2 and Mansoor Rasheed

...ddressed is an empirical analysis of contribution of such initiatives in the welfare of masses through poverty reduction in the area. This research endeavor is designed to figure out the impact of one of such projects named as Azad Jammu and Kashmir Community Development (AJKCDP) through poverty estimation and exploring the correlates of poverty in AJ and K. Multistage sampling technique was used to draw a sample of 540 respondents from the universe comprising...
Urosevic Milivoje1, Matejevic Milosava2*, Drobnjak Darko1 and Ozkanal Umit3
... (FCI). Research and analysis of morphometric parameters are required in order to write an official breed standard and recognize this breed. Previous research on this sighthound breed has shown only average values of individual morphological parameters and differences in them depending on gender, age or place of origin. However, the premise of this research is that there are several different varieties of Tazi, depending on the proportions of the body. Mea...

Faisal Hafeez, Asad Aslam*, Ayesha Iftikhar, Afifa Naeem, Muhammad Faheem Akhtar and Muhammad Jawad Saleem 

...dition to this, economic analysis of both years exhibited the similar descending order/trend in benefit cost ratio (BCR) as above. So the conservative strategies of using non-selective and conventional insecticides for the control of sucking fauna, especially against jassid, must be transformed / amended to most selective insect growth regulators (IGR’s) and new chemistry insecticides, that are safe and harmless for nature and environment. 

...
Aamir Mushtaq1,2, Rukhsana Anwar1 and Mobasher Ahmad1,2,* 
...y and quantitatively for analysis of phytoconstituents along with anti-oxidant activity. Anti-amnesic activity was found by behavioral and biochemical studies for which male albino mice were grouped into seven groups. Mice were subjected to elevated plus maze, hole board and light dark paradigm on (day 7th and 8th), for behavioral studies after which their brain were removed by decapitation for biochemical assessment. Results indicated th...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...hen were examined. Semen analysis included viability, DNA defragmentation and motility of spermatozoa were being estimated. The addition of 2μg / mL of the GnRH analogue significantly (P <0.05) improved the percentage of live spermatozoa compared to the control group. Samples with supplemented of 2 i 4 µg/mL of buserelin has a significant positive influence on percentage of motile sperm, progressive motility and DNA damage. Improving the qua...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
...followed by western blot analysis (WB) with artificial antiserum. A total of 105 Haemaphysalis longicornis (H. longicornis) ticks were selected to prepare protein samples. Some of them were used for 2-DE, and the others were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protein spots representing 12 ORFs: 4 from ticks and 8 fro...
Hui-Ying Chen1,2*, Ping-Chuan Yin1,2, Ya-Nan Lu1,2, Hai-Yun Li1,2*and Yang Shan3
...unctionally enriched for analysis. Additionally, a protein-protein interaction network (PPI) was constructed and block analysis was performed using STRING and Cytoscape databases. A total of 248 genes were identified, of which 84 were downregulated and 164 were upregulated. Functional and pathway enrichment analyses indicated that upregulated genes were significantly involved in pyrimidine metabolism, glyoxylic acid metaboli...
Fermín López-Uriostegui1, Jesus T. Ponce-Palafox2*, Fabiola Lango-Reynoso1, María R. Castañeda-Chávez1, Itzel Galaviz-Villa1, Sergio Castillo-Vargasmachuca2 and Arturo Ruiz Luna3
...xtremes of response. The analysis of response surfaces showed that the final weight and weight gain increased as temperature increased and, in the upper end, salinity had a linear synergistic effect, with a greater effect on growth at high temperatures. M. americanum attains its best growth in freshwater and low salinities(< 11‰) in subtropical zones. 
...
Mahvish Rajput1, Abdullah G. Arijo1, Muhammad Bachal Bhutto1Rehana Shahnawaz Buriro2, Javaid Ali Gadahi1, Muhammad Naeem3 and Zubair Ahmed Laghari1*
...ar characterization with analysis of their evolutionary relationship. A total of 200 rumens from slaughtered sheep were examined and out of 200 sheep, a total of 75 (37.5%) were positive for rumen fluke infection. The results indicate that sheep were infected with the morphologically identical species indicating the only species infecting rumen. Further, it was found that the infection was prevalent in all months sampled but the highest infection rate was obse...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
...l and 16s rRNA molecular analysis identified Streptococcus iniae as the causative agent of the disease outbreak. The bacteria has a unique biochemical profile compared with most of the previously isolated strains. Antimicrobial susceptibility tests revealed that S. iniae HNM-1 is resistant to most of the important antibiotics, including Kanamycin, Amoxicillin, gentamycin, Penicillin G and spectinomycin. The bacteria is pathogenic to Chinese ...
Sohail Ahmed1,*, Muhammad Waseem Saleem1, Abid Ali1, Muhammad Shahid Nisar2, Rashad Rasool Khan1 and Abdul Rashid3

Bala Gambo Jahun1,2*, Muhammad Yamin2,3, Desa Bin Ahmad2, Muhammad Razif Mahdi2, Shamsuddin Suleiman4 and Salihu Ahmad Abdulkadir5 

H. Liu1, X.P. Tang1, R.J. Fang1,*, F.Yi2, C. Zhang2, R.Q. Yang1, F. Sun1 and S.Y. Zhou1 

...ding to linear analysis. 2) compared with the control diets, supplementing the diet with Av-Cu can decrease diarrhea incidence of piglets during the whole experimental period (P < 0.05). 3) Av-Cu has significant effect on serum Cu-Zn SOD activity on day 14 (P < 0.05). This study showed that, oral administration Av-Cu to weaned piglet has the potential to promote growth performance and antioxidant capacity, and reduce diarrhea incidence.<...

Filza Fatima Rizvi1, Muhammad Umair1, Muhammad Kahlid Jamil2*, Masooma Hassan2, Komal Qayyum1 and Syed Mohsin Raza Kazmi

...nt sources. Based on the analysis, it is estimated that the solar energy potential in Pothohar region is ~ 97321 MW, only ~ 126 MW (0.12 %) is used in Agriculture sector for different farm operations. Likewise, only 51 MW of wind energy is exploited from 63000 MW, and the potential of biogas is ~ 331, 239 m³/day (from live stocks and poultry only). It is deduced from the data that only 0.2% of total available renewable energy is used, whereas 99.8% non-re...
Mian Adnan Kakakhel1, Faryal Gohar1, Zahid Anwar2, Raza Ullah1, Muhammad Attaullah3, Shahbaz Ahmad4, Aamir Khan5, Kalimullah5*
...(ELISA), and statistical analysis carried out. Out of these tested samples, 20% were positive for both IgG and IgM against T. gondii. The positive prevalence of T. gondii was 11.11% in male dogs and 42.85% in female dogs. Further details of results are given in below in main data. The finding results indicate that the seroprevalence of T. gondii is high and it is a posing concern to pu...

Noor Islam1*, Rashid Mahmood1, Ghulam Sarwar1, Sarfaraz Ahmad2 and Saleem Abid

...es and honey yield. Data analysis showed significant difference among all diets tested and check (i. e. control colonies). Diet 2 induced the highest consumption rate (71.90 g per colony), maximum sealed worker brood area (1562.0 cm2 per colony), mean highest maximum (12 frames covered with bees per colony), and higher (9.2 kg Zizyphus spp. honey per colony) as compared with control (D 5) and other tested diets. Honey bee colonies fed over Diet 2 gave a maximu...

Iram Fatima1*, Imran Pasha1, Ambreen Saddozai2, Shahid Nadeem3, Amer Mumtaz4 and Saqib Jabbar4 

Wajiha Anum1*, Liaquat Ali1, Umair Riaz2*, Abid Ali1, Nadia Manzoor3, Laal Hussain Akhter4, Asad Ur Rahman5, Naeem Maan6 and Ijaz Ahmad

...efficiency, and economic analysis. The results revealed that the band placement method gave the highest yield in comparison to the broadcast at sowing and broadcast at first irrigation. The best P fertilizer rate was identified as 150-120-60 NPK kg/ha. It has been recommended that wheat, when sown under band placement @ 120 kg/ha, gave maximum grain yield. 

...

Muhammad Rasheed1*, Zafar Ullah1, Muhammad Ansar1, Asma Hassan1, Shahzada Sohail Ijaz2, Muhammad Hussain Shah4 and Muhammad Arshadullah

... optimistic. In economic analysis MT depicted the highest net benefit per hectare over both other tillage systems that are conventional and zero-tillage with values of 632.87 $ ha-1 and Rs.152.22 $ ha-1, respectively for oats and vetch. Forage growth and yield were improved for oats in oats-vetch mixture under minimum tillage while vetch performed well in crops mixture stands. Forage yield improved by oats-vetch mixture improved agronomic practices. Maximum fo...
Majeeda Rasheed1*, Tanveer Akhtar1, Nadia Mukhtar2, Muhammad Furqan Shahid2, Muhammad Imran3, Saima Yaqub2
...currence and risk factor analysis of PPR in small ruminants (n=1000) originating from different places in district Gilgit using Hem agglutination Inhibition (HI) test followed by risk analysis through Open-Epi software. Serum samples including goats n=500 and sheep n=500 were collected from different herds situated at Naltar lake, Tattovat, Fairy meadows, Bangle, and Naltar. Overall a comparable prevalence was identified for...
Ayesha Iqbal1, Ghulam Sarwar1, Abdul Majid Khan1*, Muhammad Tahir Waseem1, Ayesha Iqbal1, Rana Manzoor Ahmad1,2, Muhammad Ameen1
...ll and data derived from analysis of our sample was compared with previously reported data of European and Japanese wild boar. As a result of comparison of the different craniometric and mandibular parameters it was inferred that the studied specimen has larger dimensions for various parameters than the European and Japanese wild boar. The smaller cranial and mandibular values in Eurasian and Japanese wild boar may be attributed to some evolutionary, climatico...

Korkmaz Bellitürk1, Zubair Aslam2, Ali Ahmad2* and Sami ur Rehman2

...he chemical and physical analysis of raw material of goat manure, cow dung, sheep manure and their vermicompost products. The earthworms for vermicomposting, Eisenia fetida (an epigeic species), were used as the test species. The samples were collected from prepared vermicompost and raw material for chemical and physical examination. For these samples, the pH, EC, organic matter, C:N ratio, phosphorus, potassium, calcium, magnesium, iron, nitrogen, sulfur and ...

Hasaan Aziz*, Fariha Altaaf, Attia Bashir, Abdul Basit, Syed Anam Masood Gardazi and Farah Haider 

...or dog at Y549, sequence analysis showed geographically distinct pattern clustering the study strain to clade Asia-2 but clearly distinct from vaccine strains. Though limited and preliminary data, the findings enhance our knowledge towards CDVs circulating in Pakistan. Further studies are much necessary to ascertain genetic characteristics of CDVs over a wide geographical area of the country together exploring the potential reasons of vaccine failure.  

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...ies and the phylogenetic analysis of unusual human rotavirus VP4 genotype detected in Moroccan children fully vaccinated with Rotarix™. After RNA virus extraction, the rotavirus VP7 and VP4 genes were amplified. The DNA was purified, sequenced and genotypes were determined using the RotaC online classification tool. A phylogenetic tree was constructed using the Maximum Likelihood method applying the Tamura 3-parameter model in MEGA 6.06 package and stati...

Olotu Titilayo Mabel*, Adeosun Idowu Jesulayomi, Kaka Mary Oluwatosin and Oladipo Elijah Kolawole 

Hafiz Muhammad Imran Javed* and Mushtaq Ahmed 

...tbreaks for phylogenetic analysis, nucleotide and deduced amino acid sequence analyses, and a comparison of three-dimensional protein structures. The N gene ORF was 1578 nucleotide long with a single open reading frame encoding 526 amino acids. Similarly, the ORF of F gene was 1641 nucleotide long which encoded 547 amino acids. Upon phylogenetic analysis of the country isolates with the vaccine strains, it was revealed that ...

Paul Oludare Adetunji* and Safiriyu Idowu Ola 

... to Spearman correlation analysis. The result showed that the plumage colour influenced (p<0.05) the onset of puberty in the cockerels with lower age at puberty, 155.29±2.41 days and 160.09±2.41 days recorded in brown and black cockerels, respectively compared to their barred counterpart, 167.23±2.41 days. Black cocks had the highest (p<0.05) semen volume (0.84±0.03ml) and sperm output (3.47±0.13×109/ejaculate) am...
Imad Hassan1, Bilal-ur-Rehman2, Muhammad Amir2, Faheem Ali2*, Salman Ilahi2 and Amjad Ali3 
...tallation and efficiency analysis. Experimental evaluation of the proposed ESP design is carried out for the removal of particulate matter from a biomass combustor. The results shown in this paper are promising and exhibit that a simple solution can achieve above 90% efficiency. The average particulate collection efficiency of our proposed ESP reached its peak stable value of 99.1% when tested with applied voltage of 25kV at an air velocity of 0.5 m/s. Moreove...

Rana Shahzad Noor1,2*, Fiaz Hussain2, Muhammad Umair2, Waqas Aslam2, Muhammad Ameen4, Muhammad Adnan Islam3 and Muhammad Sheraz2 

 

... and to perform economic analysis. Two years study results showed that the main crop peanut yields changed between 4331.0 kg ha-1 and 5486.6 kg ha-1, while second crop peanut yields changed between 2670.6 kg ha-1 and 3388.8 kg ha-1. The highest yield for main crop and crop peanut under different tillage methods was 2097.8 kg ha-1, while the lowest yield was observed as 1649.9 kg ha-1 respectively. The fuel consumption of main crop was measured between 33.33 l ...

 Salman Khan, Irfan Ullah*, Shahid Ali and Murtaza

... 14965 respectively. The analysis showed that on average per acre yield of potato was recorded as 4,953.35 kg with net revenue of Rs. 28,261.89. The results of the Cobb-Douglas type production function revealed that the coefficients of seed, labor, tractor, urea and chemicals were positive and statistically significant having elasticities of 0.211, 0.110, 0.103, 0.073 and 0.064, respectively. The study recommends that government should subsidize the inputs and...

Abdul Raqeeb1,3, Syed Moazzam Nizami2,4*, Aamir Saleem3, Lubna Ansari3, Saeed Gulzar3, Basit Ali3, Masooma Saleem5
 

...me. However, statistical analysis pointed out several significant differences in biomass allocation among C. deodara tree due to stand characteristics, highlighting the role and importance of microenvironment in regional and national forest inventories. The study provides a comprehensive tool for estimating volume and biomass, thereby resulting in an accurate assessment of carbon sequestration and stocking in cedar dominated forest ecosystem in the region.&nbs...

Javed Iqbal1, Ali Zohaib1*, Muzzammil Hussain1, Adnan Bashir1, Muhammad Hamza2, Wardah Muzaffer3, Muhammad Tahir Latif1 and Naeem Faisal1 

...died traits. Correlation analysis revealed that grain yield was positively correlated with number of productive tillers per m2 during all years and grain weight during first year; while, negatively correlated with number of grains per spike during second year indicating that increase in grain yield was associated mainly with increase in number of productive tillers per unit area in response to seed rate. In conclusion, increase in seed rate improved the grain ...

Ehsan-Ul-Haque1, Akbar Hayat1*, Muhammad Asim1, Sajjad Hussain2, Muhammad Shakeel Hanif3, Muhammad Zubair1, Muhammad Abdullah Jamil1 and Faheem Khadija1 

...e during the fresh fruit analysis undertook prior to storage. Kinnow and Willow leaf didn’t differ significantly in juice percentage (46.33 and 43.16), total soluble solids (TSS) (11.20and 10.23), percent acid (0.69 and 0.73), total sugars percent (10.75 and 9.83), crude protein, crude fat and in DPPH activity respectively. The study depict that incorporation of salts to wax in substitution to the fungicide is an effective application to control postharv...

Kajol, A. H. Bhat, Aasha and A. K. Chaubey

Biochemical and molecular characterization of Photorhabdus akhurstii associated with Heterorhabditis indica from Meerut, India

Muhammad Ismail1, Shahid Maqsood1*, Abdul Shakoor2, Feroz Shah2 

DESIGN AND COST ANALYSIS OF MOLD MAKING FACILTY AT GHANI GLASS INDUSTRY: A CASE STUDY
... is calculated. The cost analysis of the modified layout was calculated by
giving a payback period also. The results show that the proposed layout performs efficiently for the annual demand. 

...

Muhammad Qasim Khan1, Syed Athar Masood1 

PRODUCTIVITY MEASUREMENT AND ANALYSIS OF A PUBLIC SECTOR AUTOMOBILE REBUILD ORGANIZATION
...). Detailed productivity analysis of the selected organization has
been carried out with specific focus on limitations of model when applied to public sector organizations alongside
reasons for low productivity. Results indicate that public sector organizations need to focus on cost minimization and
resource optimization to enhance productivity and output as compared to private sector organizations which focus
on cost minimization a...

Muhammad Amjad*1, Abdur Rafai1, Saeed Badshah1, Rafi Ullah Khan1, Sajjad Ahmad1 

FINITE ELEMENT ANALYSIS OF THE REAL LIFE LOADINGS ON THE TI-27NB HIP BONE IMPLANT
...d in this study. The FEA analysis was performed using commercial FEA software ANSYS. The implant model
was meshed using tetrahedral element type. The simulation result show that maximum stress occurs at the neck of
the implant for each loading case. The stress level was approximately equal to the strength of Ti-27Nb in 100 Kg,
stand up position case. 

...

Mahmood Alam Khan1, Muhammad Yaseen1, Mujahid Khan*2, Amjad Khan1 

TEMPORAL VARIATION IN GROUND WATER QUALITY DUE TO URBANIZATION. CASE STUDY: DISTRICT PESHAWAR, PAKISTAN
...ared. Comparative
analysis of both the studies showed that some of the chemical parameters values gained an increase while other got
decreased. Results also showed that electrical conductivity (EC), Cl-, SO4-2, NO3-1 were increased by 119.11%,
29.98%, 469.60%, and 59.47% respectively, while total dissolved solids (TDS), turbidity, total alkalinity, total hardness,
calcium (Ca++), HCO3-1 and Magnesium (Mg++) were decreased by 6%, 10....

Zeeshan Al Hameed1, Junaid Saleem2*, Syed Sajid Hussain1, Ahsan Abdul Ghani2, Hira Lal2 

STUDY OF INDIGENOUS FLUORSPAR AS METALLURGICAL FLUX
...gical, and spectroscopic analysis. Further, indigenous
Fluorspar was applied as flux in the ladle refining operation of a local steel mill of Pakistan and results were stated
on the basis of degree of desulphurization and different basicity indexes. 

...

Zulfiqar Ali Soomro1* 

NOISE CONTROL FILTRATION OF LATERAL AND YAW DYNAMICS FOR RAILWAY VEHICLE WHEELSET ON TRACK
... lateral and yaw dynamic analysis is observed by implementation of fuzzy
inference system through applicable member functions.

...

Sahib Khan1, Muhammad Naeem2, Tawab Khan3, Nasir Ahmad4* 

4LSB BASED DATA HIDING IN COMPLEX REGION OF DIGITAL IMAGES AND ITS EFFECTS ON EDGES AND HISTOGRAM
...histogram difference steganalysis. The
quality of the edges is preserved and a PNSR of greater than 74dB has been observed for different cover images
for the true edge based 4LSB steganography. The quality of stego images, edges can been observed qualitatively
and it be seen clearly that no significant changes are introduced due to hiding information in edges using the true
edge based steganography. 

...

 M. Ihsan Ul Haq1, Ahmad Hussain2*, Farzana Yasmeen3, Shafiq R. Qureshi4

LIFE CYCLE COSTING AND PAYBACK PERIOD EVALUATION OF A SOLAR THERMAL DESALINATION SYSTEM
... In this paper, economic analysis
of the solar thermal desalination system of saline water is presented. The unit cost of desalinated water is found to
be US$ 0.0147 per litre. The energy and emission payback (EEP) period for vacuum chamber and solar collector
has also been presented. The energy payback period of solar collector and vacuum chamber are found to be 1.3
years and 1.5 years respectively. The emission payback period of s...

Zahooruddin1*, Muhammad Altaf1, Ayaz Ahmad1, Farooq Alam1 

GRADIENT ASCENT INDEPENDENT COMPONENT ANALYSIS ALGORITHM FOR TELECOMMUNICATION SIGNALS

Muhammad Naeem1*, Sahib Khan2, Nasir Ahmad3

A DISTRIBUTED GENETIC ALGORITM AND A-PRIORI ALGORITHM FOR THE HUB AND FACILITY LOCATION PROBLEMS
...mployed in market basket analysis to understand the purchase
behavior of buyers. It has many other applications. In this study, we combine a-priori with a genetic algorithm (GA)
to solve two classical NP-hard location problems namely the Un-capacitated Single Allocation Problem (USAHLP)
and Un-capacitated Facility Location Problem (UFLP). A distributed model of the proposed algorithm has been
implemented. The performance of the algo...

Nabeel Maqsood1,2*, Afzal Khan1, Muhammad Khalid Alamgir2, Shaukat Ali Shah1, Muhammad Fahad3 

PTFE THIN FILM COATING ON 316L STAINLESS STEEL FOR CORROSION PROTECTION IN ACIDIC ENVIRONMENT
... while the compositional analysis performed through energy dispersive x-ray spectroscopy (EDX).
The morphology of the coated and uncoated substrates were also studied before and after electrochemical corrosion
test and then compared. The thickness of the coating was also examined well. The result shows the remarkable
improvement in the corrosion resistance of PTFE coating by decreasing the corrosion current density in HCL media.

...

Misbah Ullah1, Rehman Akhtar1, Sikandar Bilal Khattak1, Khawar Naeem1 and Khizar Azam2 

FACTORS IDENTIFICATION FOR COAL BRIQUETTES MANUFACTURING USING STATISTICAL EXPERIMENTAL DESIGN APPROACH
...ial design approach with analysis of variance (ANOVA) is deployed to get the
optimum values of the response variables under the given conditions.

...

Waqas, A. Imtiaz1, Yousaf Khan1, Syed Waqar Shah2 

COST VERSUS RELIABILITY ANALYSIS OF NEW TREE-BASED HYBRID PROTECTION ARCHITECTURE FOR OPTICAL CODE DIVISION MULTIPLE ACCESS SYSTEM
...> focuses on design and analysis of a novel tree-based hybrid protection architecture for OCDMA system in order to
make it economical and reliable for deployment at the access domain. It is observed that the proposed protection
architecture provides desirable (5 nines) connection availability along with minimum cost as compared to existing
tree based protection architectures. 

...

Kulsoom Iftikhar1, Shahzad Anwar1, Izhar Ul Haq1, Muhammad Tahir Khan1 and Sayed Riaz Akbar1 

AN OPTIMAL NEURAL NETWORK BASED CLASSIFICATION TECHNIQUE FOR BREAST CANCER DETECTION
...works comparative
analysis is performed to study the optimum parameters required for prime mass classification. The execution
of suggested methodology is estimated using ROC curve. The accuracy rate of developed method is 93.1% or 0.93
with sensitivity of 0.99 and specificity of 0.83 according to the receiver operating characteristic (ROC). 

...

Aqeel Nawaz1, Muhammad Ali Kamran1 

PARAMETRIC SENSITIVITY ANALYSIS OF CONVEX STAR PORT PROPELLANT GRAIN FOR SOLID ROCKET MOTOR
... profile for qualitative analysis of
sliver fraction (tail-off)and neutrality. The purpose of the study is to expand the design domain by evaluating entire
convex Star family under both neutral and least sliver content conditions.The computer program associated to it
is essentially the ballistic design analysis of the convex star grain configuration.Results showed that neutrality and
sliver fracti...

Ghani Akbar1, Muhammad Munir Ahmad1, Abdul Ghafoor1, Matiullah Khan1, Zafar Islam1 

IRRIGATION EFFICIENCIES POTENTIAL UNDER SURFACE IRRIGATED FARMS IN PAKISTAN
...> length and width. The analysis demonstrated increased understanding of the interactions of field sizes and irrigation
efficiencies, which may support irrigators’ decision making for enhancing irrigation performance at no significant
cost to infrastructure, machinery or labour. 

...

Nazish Huma Khan1, Mohammad Nafees1, Adila Bashir1, Farooq Ahmad1 

STUDY OF POLLUTION LAOD IN PAPER MILL EFFLUENTS AND ITS RECYCLING BY SUNDRY PLANTS AT HAYATABAD INDUSTRIAL ESTATE, PESHAWAR

Muhammad Ibrahim1, ShahidMaqsood1, Rafiullah Khan2, Muhammad Amjad2, SakhiJaan2 

OPTIMIZATION OF GAS TUNGSTEN ARC WELDING PARAMETERS ON PENETRATION DEPTH AND BEAD WIDTH USING TAGUCHI METHOD
...r /> determined through analysis of variance approach. The results showed that the penetration depth and bead width
were increased with increase in current and decrease with increase in torch speed. It was concluded that the arc
current is the significant factor for both the response parameters while torch angle is significant only for bead width.
The results have been verified through confirmation test for each response parameter. <...

Salman Saeed*, Akhtar Naeem Khan**, Rashid Rehan*, Sikandar Hayat Sajid**, Muhammad Sagheer Aslam*, Khan Shahzada** 

COST AND PERFORMANCE OPTIMIZATION OF PRECAST POST TENSIONED PRE-STRESSED GIRDER BRIDGE SUPERSTRUCTURES
...adsheet is developed for analysis and design of deck slab and girders using one dimensional
beam line analysis and AASHTO LRFD distribution factors. The spread sheet also calculates the overall cost of the
bridge superstructure. This spreadsheet is then used to perform a parametric study by iterating through all design
parameters. It is shown that, contrary to conventional wisdom, the cost and performanc...

Rahim Haider*, Al Mustaan Kakar*, Sikandar Bilal Khattak*, Safi ur Rahman**, Shahid Maqsood*, Misbah Ullah*, Rehman Akhtar*, Aamir Sikandar* 

DEVELOPMENT OF OPTIMIZED MAINTENANCE SYSTEM FOR VEHICLE FLEET
...ntenance strategies. The analysis is carried out on the basis of
likelihood of the failures. Five Why Analysis is used to identify the root cause. The model incorporates the inspection
method by using checklists and maintenance schedules. The system is capable to provide the basis for an advance
system development. 

...

Anwar Ullah*, Sabir Islam*, Sikandar Bilal Khattak*, Safi Ur Rahman**, Shahid Maqsood*, Misbah Ullah*, Rehman Akhtar*, Rashid Nawaz* 

MAINTENANCE SYSTEM FOR HEAVY EARTH MOVING EQUIPMENT
...ronment it works in. The analysis includes segregation, Pareto analysis and Five Why analysis. A dedicated
maintenance model is proposed which includes checklist and proper maintenance schedule. 

...

Farid Ullah Khan*, Adeel Ahmed*, Uzair Khan Jadoon*, Fahim Haider* 

MODELING, SIMULATION AND FABRICATION OF AN UNDERSHOT FLOATING WATERWHEEL
...ons. It is found, during analysis that the water flow velocity is the
major factor affecting the output power and due to availability of high flow velocity stream the parameters of the
waterwheel, such as, radius and width of wheel can be reduced considerably. For a flow velocity of 1.5 m/s, design
estimates for waterwheel producing 1 kW power are obtained with the devised analytical model. The simulations
performed for the 1 kW pow...

Suhaib*, Shahid Maqsood*, Sikandar Bilal Khattak*, Misbah Ullah*, Rehman Akhtar*, Rashid Nawaz*, Iftikhar Hussain*, Abdul Shakoor**, Khizar Azam** 

SINGLE FACILITY LOCATION SELECTIONPROBLEM FOR A PAKISTAN BASED ICE CREAM COMPANY: A CASE STUDY
...raging results. The cost analysis of each potential
location is also presented in this paper. 

...

Imran Shehzad1, Ghulam Sarwar1*, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Sher Muhammad2 and Ghulam Murtaza

...re and post-harvest soil analysis was carried out for different physical and chemical characteristics. Soil samples were collected from all the plots and analyzed for different chemical properties like EC, pH, SAR, Cl, CO3, HCO3, Ca, Na, Mg, N, P and K. Results of the experiment revealed that use of saline water irrigation impaired different chemical properties of the soil and also had negative impact on fertility status of the soil. All the collected data wer...

Muhammad Shahid1, Adiqa Kiani2* and Mahmood Shah3

Livestock Tackles the Major Sector Fluctuations in Agrarian Sector and Raises Economic Performance: A Study of Pakistan
...er focuses the ambiguous analysis of livestock sector in raising the level of economic performance. The study reveals implications of livestock in tackling seasonal fluctuations, inputs shocks and failure of crops in major sector by taking time series data from 1984 to 2017. Auto regressive distributive lag (ARDL) econometric technique was applied to conclude empirical results. Further, cumulative sum (CUSUM) and CUSUM square test was used to check the stabili...

Waqas Anwar, Iftikhar Hussain 

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...ence using multi-variant analysis for low productivity in restive regions. The author uses primary dataset and seeks feedback from the respondents through a comprehensive questionnaire containing twenty-six questions. Comparing circumstances and outcome of construction projects with remaining part of the country, the study finds that the cost of work in FATA / PATA and restive regions of Baluchistan is substantially high due to multiple factors and therefore r...

Waqas Anwar, Iftikhar Hussain 

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...ence using multi-variant analysis for low productivity in restive regions. The author uses primary dataset and seeks feedback from the respondents through a comprehensive questionnaire containing twenty-six questions. Comparing circumstances and outcome of construction projects with remaining part of the country, the study finds that the cost of work in FATA / PATA and restive regions of Baluchistan is substantially high due to multiple factors and therefore r...

Muhammad Shahid1, Adiqa Kiani2* and Mahmood Shah3

Livestock Tackles the Major Sector Fluctuations in Agrarian Sector and Raises Economic Performance: A Study of Pakistan
...er focuses the ambiguous analysis of livestock sector in raising the level of economic performance. The study reveals implications of livestock in tackling seasonal fluctuations, inputs shocks and failure of crops in major sector by taking time series data from 1984 to 2017. Auto regressive distributive lag (ARDL) econometric technique was applied to conclude empirical results. Further, cumulative sum (CUSUM) and CUSUM square test was used to check the stabili...
Kemi F. Omotesho*, Adedamola V. Adetayo, Adeniyi F. Akinrinde and Deborah A. Olabode
 
Challenges to the Adoption of Agricultural Innovations: The Case of Yam Minisett Technology in Kwara State, Nigeria

Parsa Riaz and Muhammad Naeem*

Digestive Enzymes Activity with Gut Morphometric Parameter of Carnivorous Fish Wallago attu (Siluridae, Siluriformes)
...and protease. Regression analysis of Protease has highly significant correlation with the entire factor except Total length. With the help of principle component analysis, fish weight was found another important trait would be investigated as dependent variable. These variables were selected on the basis of multicollinearity and principle component analysis interpretations. Present study m...

Hina Fatima1*, Sania Shaheen2, Lal Khan Almas3 and Sehrish Haroon4

Profit Efficiency among Transplanting and Direct Seeded Rice Producers (Case Study of Certain Rice Growing Areas of Province Punjab, Pakistan)

Ihsan Rabbi*, Sehat Ullah**, Aftab Alam**

MARKER BASED TRACKING IN AUGMENTED REALITY APPLICATIONS USING ARTOOLKIT: A CASE STUDY
...his paper, we present an analysis of marker-based tracking using ARToolKit. We investigate the
effect of marker size, distance between markers and camera, the speed of marker with respect to camera, relationship
between marker size and distance, the brightness and contrast level of a camera on tracking a single marker.
Experiments were conducted to produce the analysis of these factors.

Waqas Anwar1*, Iftikhar Hussain1

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...ence using multi-variant analysis for low productivity
in restive regions. The author uses primary dataset and seeks feedback from the respondents through a comprehensive
questionnaire containing twenty-six questions. Comparing circumstances and outcome of construction projects with
remaining part of the country, the study finds that the cost of work in FATA / PATA and restive regions of Baluchistan
is substantia...

 Sajjad Hussain1*, Nisar Mohammad1, Zahid Ur Rehman1, Noor Mohammad1, Ishaq Ahmad1,

Naseer Muhammad Khan2 and Salim Raza1
EXTRACTION OF GOLD AND SILVER BY AGITATION CYANIDATION PROCESS FROM COMPLEX ORE OF SHOUGHUR AREA CHITRAL, KYBER PAKHTUNKHWA PAKISTAN
...Based on the comparative analysis of result it is concluded that cyanidation process on roasted ore samples gives
better results as compared to un-roasted ore samples.
...

Nisar Mohammad*, Mohammad Mansoor Khan* Noor Mohammad*

CHARACTERIZATION OF TALC-CARBONATE ROCK OF MINGORA EMERALD MINES, DISTRICT SWAT, KHYBER PAKHTUNKHWA, PAKISTAN
...">by XRF spectrometer analysis. Grinding optimization study of the host rock using rod mill was also done. Whiteness of original talc carbonate rock and its magnetic content was also determined. The investigations reveal that talc content in the tal...

 Khizar Azam*, Abdul Shakoor*~ Afzal Khan*, Riaz Akbar Shah*~ Shaukat Ali Shah·

IDENTIFICATION OF VITAL VEHICLE MECHANICAL DEFECTS CAUSING ROAD TRAFFIC ACCIDENTS IN PAKISTAN
...normal;">quantitative analysis of RTA on Motorways in Pakistan caused due

 Muhammad Khurram Ali*, Raft Javed Qureshi**, Mirza Jahanzaib***

SELECTING FACILITY LOCATION USING HYBRID METHODOLOGIES IN GLOBAL PERSPECTIVE: A CASE OF CEMENT PLANT
...rucial decision requires analysis of wide range offactors with different optimization techniques. Therefore such factors were explored and prioritized from available literature and by recommendations ofpanel ofexperts followed by mathematical modelling of the said problem in four standard methodologiesincluding the one presented in another 

Farid Ullah Khan*

MINIATURE VIBRATION SHAKER FOR MEMS-SCALE VIBRATION-BASED ENERGY HARVESTERS APPLICATION
...nal to the shaker. Modal analysis of the suspension system performed in COMSOL Multiphysics®, indicates that in the first mode of the vibration the shaker’s table is perfectly moving up and down. The developed vibration shaker is characterized for sinusoidal electrical input signal. At different gain levels of the power amplifier, the shaker is subjected to a frequency sweep from 1 Hz to 1 kHz. At resonant frequency of 60 Hz, acceleration amplitudes ...

M A Khalid*, A R Ghumman**, H. N. Hashmi**, E. Tanveer*

INVESTIGATION OF PERFORMANCE AND SEDIMENT TRANSPORT OF LOWER BARI DOAB CANAL
...is on sediment transport analysis has been investigated using field data. The performance of canal has been discussed using delivery performance ratio (DPR) a well known performance indicator. The sediment laden water flow problem may be described by three equations; the one dimensional equation of motion (momentum-equation) and the flow and sediment-continuity equations. All the three equations are partial differential equations and may be solved by the finit...

Muhammad Waseem*, Irshad Ahmad**, Rawid Khan**, Bashir Alam**, Khan Shahzada**, Muhammad Javed**

STABILITY ANALYSIS OF SOIL SLOPE IN SWABI, PAKISTAN
...s study, slope stability analysis for a particular type of soil in Swabi area was carried out using the method of slices. Gravity load and seismic load were considered in the stability analysis. SLOPE/W and SIGMA/W software of Geo studio package were used for analysis under both dry and completely saturated conditions. Pseudo-static approach was used for the seismic stability

Azam Khan*, Irshad Ahmad**

TIME HISTORY ANALYSIS OF SEISMICALLY ISOLATED FIXED BASE OFFSHORE PLATFORM
...l nonlinear time history analysis (including soil and foundation modelling) has been undertaken using finite element software SAFJAC for an extreme earthquake records scaled for the DLE event.
...

Azam Khan*, Irshad Ahmad**

IN-PLACE ANALYSIS OF JACKET OFFSHORE STRUCTURE UNDER STORM CONDITIONS
... the details of In-place analysis of fixed jacket Platform BD. The numerical model is prepared using ANSYS ASAS structural analysis computer system. Beam stick model has been used, with multi-level sub-structuring technique, to model the whole structure. Appropriate dead load, live loads and quasi-static action of 100 yr storm have been applied to the structure. The structure-pile interaction

Tahir Usman*, Khan Muhammad*

MODELING OF BLASTING COST AT DEEWAN CEMENT QUARRY, HATTAR USING MULTIVARIATE REGRESSION

Muhammad Naeem*, Sohail Asghar**

A REVIEW OF FEATURE SELECTION TECHNIQUES IN STRUCTURE LEARNING
... eleven evaluators. This analysis is useful in inducing better structure from the given dataset in imparting improved performance metric for Bayesian belief network.
...

Muhammad Irfan*, Saeed Gul*, Mohammad Younas*

SOLAR STILL PERFORMANCE FOR SEA WATER PURIFICATION: A STATISTICAL AND EXPERIMENTAL STUDY
...tistically though ANOVA (analysis of variance). It was found that the water temperature in the still is the most significant parameter that largely affects the evaporation rate. The fresh water with 240 mg/L dissolved salt has been extracted from the model sea water having dissolved salts of 35000 mg/L. The efficiency of a single basin solar still is improved up to 80 percent. 
...

 Amjad Ali*, Amir Nawaz Khan*, Atta-ur-Rahman*, Muhammad Saeed*

ANALYSIS OF SITE, SITUATION AND THEIR IMPACT ON RESETTLEMENT OF PROPOSED NEW BALAKOT TOWN, PAKISTAN: AN EX POST EVALUATION OF 2005 KASHMIR EARTHQUAKE
...ttempts to carry out the analysis of site, situation and their impact on resettlement of proposed new Balakot town, Pakistan. The site analysis provides details of vulnerability to hazards, civic utilities and services. The new Balakot is a planned town and have low vulnerability to seismic hazards. The situation analysis explores the potential growth and development of the ...

Hazrat Hussain*, Muhammad Ibrahim**, Navid Ahmed***, Zia ul Haq**, Bashir Alam***

ASSESSMENT OF SEDIMENT TRANSPORT LOAD FROM GOAML RIVER AND ITS IMPACT ON THE GOMAL ZAM DAM LIFESPAN
...ata and using regression analysis a model is developed to establish a correlation between water discharge and sediment load. The model is an attempt to ascertain the rate of erosion in the catchment of the Gomal River since no generalized model has so far been developed to explain the nature of such weathering activities and all such works are situation specific. Relation between the water discharges to sediment load that it carries has bee...

Asif Nawaz*, Muhammad Zafarullah Khan, Rehmat Ullah, Khalid Nawab and Urooba Pervaiz

Investigation of Professional Competency Level and Training Needs of Field Assistants in Khyber Pakhtunkhwa
...ting farmers. Regression analysis showed that rural domicile, job experience, and higher educational level of Field Assistants have positive and significant effect on the development of their competency. Therefore, it is suggested to provide in-service training for the Field Assistants in the identified areas for the adequate acquisition of relevant skills and knowledge.

...

Fazli Rabbi1*, Muhammad Idrees2, Shahid Ali1, Muhammad Zamin3 and Hazrat Bilal4

Farmers Perceptions and Adoption of Information and Communication Technologies (ICTs) in Peach (Prunus persica L.) Production and Marketing
...tests were used for data analysis. The results showed a greater awareness level among the peach (P. persica L.) growers about ICTs. Moreover, the analysis indicated that more than 75 % of farmers are in use of a single or multiple type of ICTs. Among the sampled farmers, 70% of the farmers perceived ICTs as useful for farming activities. The analysis also revealed that 58.5% of the farmers...

Muhammad Riaz1*, Naureen Akhtar1, Salik Nawaz Khan1, Muhammad Shakeel1,2 and Ateeq Tahir1

Neocosmospora rubicola: An Unrecorded Pathogen from Pakistan Causing Potato Stem Rot
...bulin gene. Phylogenetic analysis was also conducted to determine the phylogenetic relationship of this species with other reported species of this genus. Pathogenic potential of the isolate was verified by artificially inoculating the spores of pathogen in healthy plants. Appearance of same symptoms and re-isolation of N. rubicola from infected tissue confirmed Koch’s pathogenicity postulates. Association of N. rubicola causing stem rot in potato is nev...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...dents was used for final analysis and description of the results. The instrument’s validity and reliability were checked before data collection. In-person interviews were conducted by the researcher. The collected data were analyzed by Statistical Package for Social Sciences (SPSS). Based on the results, it is concluded that majority of the farmers were not willing to adopt IFS due to their traditional perceptions. Those farmers have constraints such as ...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...dents was used for final analysis and description of the results. The instrument’s validity and reliability were checked before data collection. In-person interviews were conducted by the researcher. The collected data were analyzed by Statistical Package for Social Sciences (SPSS). Based on the results, it is concluded that majority of the farmers were not willing to adopt IFS due to their traditional perceptions. Those farmers have constraints such as ...

Mohammad Aslam Khan*, Samiullah* and Zulfiqar Ali*

DYNAMICS OF URBAN SPATIAL GROWTH AND PLANNING: A CASE STUDY OF PESHAWAR
...nd satellite images. The analysis shows that the city has been growing at a rapid pace adding about 500 ha of land annually in recent years and in the absence of planning control has seen tremendous urban sprawl. The pattern of development of urban sprawl in Peshawar is typical of most Pakistani major cities, where ribbon sprawl is common along major roads, while leapfrog sprawl is prevalent in the city’s fringes. The uncontrolled exp...

Asadullah Saleem* and Nadeem Ehsan*

STATUS OF PERFORMANCE MEASUREMENT SYSTEMS IN INFRASTRUCTURE CONSTRUCTION FIRMS OF PAKISTAN

Sikandar Khan*, Afzal Khan*, Muhammad Tahir Khan*, Muhammad Ali Kamran* and Abdul Shakoor*

DYNAMIC MODELING AND STARTING BEHAVIOR OF A SMALL HORIZONTAL AXIS WIND TURBINE
... the output torques. Our analysis shows that most of the starting torque comes from the hub section of the blade. Various changes were incorporated into ADAMS models near the hub region of the wind turbine blade which showed an increase in the staring torque due to an increasein the blade angles and chord lengths. The wind turbine designs for the selected locations of Pakistan were successfully optimizedby improving their starting behavior....

Behram Khan1, Amir Nawaz Khan2, Said Qasim3, Atta-ur Rahman4

ANALYSIS OF HUMAN PERCEPTIONS AND ADAPTATIONS TO FLASH FLOODS IN KHYBER AGENCY, FATA
...es, maps and plates. The analysis reveals that people of the area perceived about the recurrence of future flash floods during rainy season. Most of the people perceived the flash floods as it hit the study area with devastating impacts and the extent of flood damages. In spite of flood risk, the level of affinity towards living in the flood prone area was found high among the community as they have blood ties with the land, kith and kinship. In the study area...

 Uzma Nawaz1*, Muhammad Iftikhar Khan1, Muhammad Naeem Arbab1

TECHNO-ECONOMICS OF UPS SYSTEM AS BACKUP POWER SOURCE DURING LOAD SHEDDING
...cles. In particular analysis shows that the operating cost of the battery increases with the number of cycles the battery undergoes. The work will form a platform for conducting studies related to UPS, for setting standards, policy and the areas where improvement is needed.

...

 Sikandar Bilal Khattak, Iftikhar Hussain, Rashid Nawaz

EVALUATING THE SOCIAL ASPECT OF SUSTAINABLE CONSTRUCTION FOR PAKISTAN VIA ANALYTICAL HIERARCHY PROCESS
...values obtained from AHP analysis. The result shows that clean water is considered in almost all of the projects whereas the universities and other research institutes are hardly considered in any projects.

...

 Majid Ashraf, Mohammad Haseeb Zafar, Tariqullah Jan

EFFICIENT ROUTING SCHEME FOR UNIDIRECTIONAL LINKS IN MULTI-HOP NETWORKS
...etworks. The performance analysis when compared with the three routing protocols to manage unidirectional links shows that our proposed ARR scheme is superior to the AODV and AODV-Blacklist.

...

 Muhammad Qasim Khan1*, Syed Athar Masood1 and Shahzad Naeem Qureshi2

REFORMULATION OF PRODUCTIVITY IMPROVEMENT MODEL FOR PUBLIC SECTOR AUTOMOBILE REBUILD ORGANIZATIONS
...ve and quantitative analysis, an inclusive productivity enhancement model for public sector automobile rebuild organizations was reformulated. Proposed model when implemented holistically in rebuild organizations can produce positive results whereas implementation of proposed practices in piecemeal/isolation may not accrue true and optimal outcomes. The findings of this research will facilitate top and middle management to enhance productiv...

 Muhammad Shahzad1, Arif Iqbal Umar1, Syed Hamad Shirazi1, Muhammad Tariq Pervez2*, Zakir Khan1, Waqas Yousaf1

HCDP: HEPATITIS C DATA BANK OF PAKISTAN
...aphic region strains for analysis. No HCV database available to find the HCV prevalence and distribution in Pakistan. We proposed a Hepatitis C virus database for Pakistani community, Hepatitis C Data Bank of Pakistan (HCDP). HCDP will be the first database that will holds HCV sequences obtained from Pakistani strains and HCV research publication by Pakistani researchers. We proposed a Hepatitis C Data Bank of Pakistan (HCDP) with an online...

 Shakil Rehman Sheikh1*, Zafar Ullah Koreshi1, Nouman Ghafoor1, Muhammad Shahid Khalil1, Muzzamil Ghaffar1

MITIGATING CO2 EMISSIONS OF ACADEMIC BUILDINGS IN PAKISTAN USING ENERGY CONSERVATION TECHNIQUES
...ed. The measurements and analysis indicated that other measures like the use of different types of window glazing, LED Lighting, efficient gas heating, and improvement in behavior pattern could also result in substantial energy savings. The maximum achievable savings can be as much as 50% of the energy cost of the school buildings. Additionally, once extrapolated over the entire school-going population of Pakistan the CO2 emission savings c...

 Nisar Mohammad1, Mohammad Mansoor Khan1

RECOVERY OF TALC FROM TALC- CARBONATE SCHIST OF SWAT EMERALD MINES, NWFP, PAKISTAN
...uation and chemical analysis were undertaken on talc bearing rock of Swat emerald mines, aiming at finding some suitable processing technique to recover talc. A few processing tests using flotation, gravity separation and leaching methods were conducted to see the amenability of talc to enrichment by these techniques. Analysis of seven talcum powder products available in the local market including pharmac...

 Faizullah Mahar1, Syed Saad Azhar Ali1, Abid Karim1

DESIGN OF FIXED ORDER ROBUST CONTROLLER USING H∞ -NORM AND EVOLUTIONARY TECHNIQUES: COMPARISONS AND PERFORMANCE ANALYSIS
...valuated for performance analysis via extensive simulations. Simulation results and performance comparisons demonstrate the efficiency of proposed approaches.
...

 Muhammad Naeem Khan Khattak1, Hamidullah1, Shehryar2

PROBLEMS, ANALYSIS AND RECOMMENDATIONS OF THE AUTOMOTIVE VENDOR INDUSTRY OF PAKISTAN—I
...bsp;presents very useful analysis and comprehensive recommendations for solution of major problems of the automotive vendor industry. The problems of automotive vendor industry are identified as a result of interviews with vendors of the industry. The problems of car vendors are divided into five major categories: processes/ operations, supply chain, resources, testing & quality control, and poor technology climate. It includes both the...

 Adnan Ali Khan,* and Shahrukh Khalid**

CAPACITY ESTIMATION OF SIMO SYSTEMS IN THE PRESENCE OF CO-CHANNEL INTERFERENCE
...mits defined by MRC analysis are found within the estimated values of capacity using simulation. It is observed that increasing cell size and re-use distance decrease the effect of co-channel interference, however, the overall area spectral efficiency (ASE) of the system reduces due to the increase in cellular coverage area. Thus, a trade-off between capacity improvement and reduction in cell-size is suggested.
...

 Usman Ali Naeem*, Hashim Nisar*, Habib-ur-Rehman** and Naeem Ejaz*

DEVELOPMENT OF FLOOD DISCHARGE MODELS USING GIS
...eans. Also the watershed analysis of the upstream of Indus river basin was performed, thus fashioning an essential tool for running and setting up of water resources for state.
...

 Muhammad Shaheen*, Muhammad Shahbaz*, Zahoor ur Rehman* and Sajid Mehmood*

A METHOD OF DATA MINING FOR SUSTAINABLE HYDROCARBON EXPLORATION
... statistical correlation analysis. After finding exploration SI, multiple regression is used to predict optimal hydrocarbon exploration rate which will ensure sustainable energy development within the country.
...

Rafi Javed Qureshi* and Iftikhar Hussain**

INTERNATIONALIZING MANUFACTURING SMEs IN A DEVELOPING REGION: AN EXHAUSTIVE CDA-BASED EXPLORATORY APPROACH
...vant to categorical data analysis (CDA) in this paper highlights the enabling role of the exploratory factors in understanding the relationship with SME’s exporting. Categorical regression overcomes the limitations of both contingency table method and binary logistic regression in exploring this relationship, and provides substantive space to experiment and investigate the contributory role of export-enabling factors when data size is...

Rashidullah*, Irshad Ahmad**

SEISMIC HAZARD ANALYSIS OF ISLAMABAD
...for deterministic hazard analysis. Seismic zoning map of NESPAK has been utilized in the research. Earthquake catalogue contains total of 11,149 events. Earthquake events of moment magnitude (Mw>4.0) are taken for developing the recurrence relationships of selected four (04) seismic source zones. The attenuation equation of Boore and Atkinson 2008 next generation attenuation (BA08 NGA) has been used for the determination of the ground mo...

 Muhammad Waseem*, Irshad Ahmad**, Muhammad Asif Khan**

DETERMINISTIC SEISMIC HAZARD ANALYSIS AND SELECTION OF TIME HISTORIES FOR DYNAMIC ANALYSIS FOR A SITE IN SWABI
...essitates seismic hazard analysis to be evaluated and a suit of acceleration time histories be selected for dynamic analysis of the slope. Three time histories are selected in this study from real earthquakes that matches parameters with the controlling earthquake.
...

Mehwish Liaquat1*, Arshad Mahmood Malik1, Muhammad Ishaq1, Hafiz Muhammad Qasim Ashraf2 and Ismara Naseem

Sinan Uzundumlu1, Yesim Buyukates1,*, Murat Yigit2, Musa Bulut2,  Rıdvan Kaan Gurses1 and Barbaros Celikkol3
... in the cage system. MDS analysis results showed that TSS, chlorophyll-a and TP were similar throughout the sampling period. The study showed that copper alloy cage system did not have any negative impact on the marine system, compared with the limits provided by FAO, EPA, WPCR and RTMAF as well as with previously conducted studies in the region.
...
Ming-Yan Shi2, Xu-Liang Yan1, Ping-Ping Han1, Yu-Jie Tang1 and Hui-Xia Li1*
...lly-expressed miRNAs and analysis of bSMSCs target genes were then carried out using microarray and bioinformatics. The data revealed significant changes (>2-fold) in 29 out of 783 differentially expressed miRNAs, 12 of which were up-regulated and 17 were down-regulated. Bioinformatics analysis showed that 24 of these 29 miRNAs were involved in fat metabolism, cell differentiation and the development of the immune system ...

Ghulam Murtaza1, Ghulam Sarwar1*, Muhammad Ashraf Malik2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1 and Sher Muhammad3 

Guang-Hui Tan, Yi-Yu Zhang*, Yuan-Yu Qin, Lei Wu and Jie-Zhang Li
...c diversity. Association analysis revealed that two SNPs were significant association with at least four of seven lipid indexes. Allele A of CDS 216 A>G locus and allele T of CDS 681 T>A locus was favorable to improving meat quality, respectively. Three haplotypes and six diplotypes were identified by the combination of two SNPs. Diplotypes had dominantly affected on tested lipid indexes except for IMF. Diplotype H1H1 was advantageous for the improvement...

Zakeer Ahmed Khan Abbasi* and Allah Nawaz 

... change. The statistical analysis of the field data supports the assumptions about positive association between climate change awareness and climate change adaptations (R2=44.6, B=.875 and P-value=.000) and negative interaction between climate change awareness and climate change adaptation issues (R2=.318, B=-.707 and P-value=.000). Further, the role of geographic-zones and respondents’ educational level has also been found significant. Therefore, more e...

Matiullah Khan1*, Humera Arif2, Muhammad Rasheed2, Motsim Billah3 and Shoaib Ahmad

... per kg RP. The periodic analysis of the reacted RP showed that availability of P increased with the time span, irrespective of the treatments and locations. However, the highest P in all reacted RP was recorded on day 90. Beyond day 90, the P content showed a declining trend, especially in reacted RP of Paswal and Kakul. These results reveal that with the change in mineralogy of RP, the requirement of H2SO4 for solubilizing P also changes hence, separate form...

Haiyan Yang1, Hongxia Liu1, Wenlong Wu1*, Weilin Li2 and Lianfei Lyu

...hosen for drought stress analysis. All plants were grown in pots under greenhouse conditions, and were subjected to 20-day drought stress by withholding irrigation, followed by re-watering for 5 days. The changes in leaf water content (LWC), electrolyte leakage (EL), concentrations of photosynthetic pigments, protein, soluble sugar, hydrogen peroxide (H2O2), malondialdehyde (MDA), ascorbate (AsA) and reduced glutathione (GSH), activities of superoxide dismutas...
Mumtaz Ali Khan1, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Kashif Prince1*, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4Asad Ullah5, Naimat Ullah4, Muhammad Shoaib2 and Said Sajjad Ali Shah2
...eters. Hematobiochemical analysis indicated that mean erythrocyte count (RBC) and hemoglobin levels decreased while the mean leukocytes (WBC), platelets, packet cell volume (PCV) and total bilirubin levels increased significantly (P<0.05) in sheep infected with Clostridium perfringens type A. Sheep infected with Clostridium perfringens type B and type D showed significant (P<0.05) decreases in erythrocytes counts (RBC) and hemoglobin levels...
Maryam Yousaf1, Naveed Shahzad2, Zeeshan Mutahir1 and Moazzam Ali1*
...qRT-PCR and Western blot analysis showed that PC-3/Res cells also had significantly up-regulated expression of Rab21. To study the effect of Rab21 on MRP-1 mediated multidrug resistance, siRNA mediated knockdown of Rab21 was performed. Results showed that the knocking down of Rab21 decreased the drug efflux ability of PC-3/Res cells, possibly by altering the surface localisation of MRP-1. These results suggest that interruption in MRP-1 trafficking to the plas...
Muhammad Khan1, Tehmina Ameer Khan1, Aziz Ud Din2, Muhammad Fiaz Khan1, Irfan Ullah3,*, Kalim Ullah4, Sadia Tabassum1,*
...T-TL1) were targeted for analysis of mutation. Genomic DNA was extracted from buccal swab using Phenol: Chloroform: protocol. The target genes were amplified via polymerase chain reaction (PCR). The amplified gene products were sequenced and compared with the revised Cambridge Reference Sequence (rCRS) Accession-No. N_012920.1. Four mutations in 16S-rRNA gene have been identified viz mt-2552T>A, mt-1811A>G, mt-1888A>G and mt-2467A>T a...
Abdul Waheed*, Asim Faraz, Hafiz Muhammad Ishaq
... descriptive statistical analysis. The farmer’s family size averaged 6.4±1.6 with average monthly income of Rs. 14322.6±3026.4. These rural household had on the average 11.4±4.5 hens, 2.1±1.1 cocks, 6.9±4.5 chicks, and 4.5±3.4 laying hens. Majority of them kept hen in khuda (a small need-based structure like small room for keeping birds). Most of them offer grains to the desi birds and majority was aware of poult...

Murtaza1, Syed Attaullah Shah1*, Shahid Ali1, Amjad Ali1 and Asia Baig2

Economic Analysis of Maize Production in Central Khyber Pakhtunkhwa, Pakistan
...e growers. Profitability analysis confirmed high net-return for hybrid maize as compared to local maize crop. The estimated Stochastic Frontier Model-1 showed that maize yield is positively affected by hybrid seed cultivation, irrigation, organic and inorganic fertilizers application. The model predicted mean technical efficiency of 85% with a maximum possible of 97%. This indicates that 12% increase in yield is possible with the existing production technology...

Erum Bughio1, Ahmed Sultan Jatoi1*, Jibran Hussain2, Muhammad Hayat Jaspal3, Shahid Mehmood2, Hafiz Muhammad Ishaq4, Reema Bughio5 and Athar Mahmud2

Comparative Study of Carcass Traits in Four Strains of Japanese Quail (Coturnix japonica) at 3 to 6 Weeks of Age
...hnique was used for data analysis and DMR Test for comparison of means. Significant (p<0.05) differences were observed in mean slaughter and liver weight (g) in quail birds. Significant (p<0.05) differences were noted in keel length (cm) at week 3, 5 and 6 and keel angle at week-3. A significant (p<0.05) effect was observed in mean heart weight at week 3 and 6, gizzard weight at week 4, 5 and 6 and intestinal length (cm) at week 3, 5 and 6;  wher...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...garose gel. DNA sequence analysis of PCR product indicated that specimens shared a 99-100% identity with Schistocerca gregaria gregaria)(Forskål, 1775). Molecular phylogenetic analysis and evolutionary divergence study also revealed the formation of same cluster in phylogenetic tree with that of specific subspecies S. g. gregaria which furtherdeviate genetically not only from closely related subspecies (<...
He Wenyun, Zhi Yijin and Shao Mingqin*
...oad. Principal component analysis indicated that water flow speed, distance to village, river width, vegetation cover, distance to mine factory and shoal proportion were the main factors for habitat selection. The results suggested that safety was the primary factor in habitat selection. The main foraging method was up-ending (61.54%); the duration of each up-ending foraging behavior was 1.92±0.83 s and foraging frequency was 14.67±4.43 times/min...
Gregory Ejikeme Odo1,*, Juliana Ekenma Agwu1, Nkechi Nweze2, Clara Ikegbunam2,Felicia Ekeh N1, Vincent Ejere1, Ngozi Ezenwaji1, Reginald Njokuocha2, Godwin Ngwu1 and Fabian Okafor1
...ere subjected to two-way analysis of variance (ANOVA) with Duncan’s multiple comparison post hoc tests to compare the level of significance between control and experimental groups. Group A served as control in which no leaf extract was administeredwhilegroupsB,Ca

Tao He1,2,3, Cheng-jing Chen4, Jian-guang Qin3, Yun Li1,2, Rong-hua Wu1,2 and Tian-xiang Gao4,*

...ped, principal component analysis for otolith shape is not possible to distinguish L. haematocheilus stocks. Based on the Fourier coefficient, Fisher’s discriminant analysis accurately classified 76.2% specimens into correct stocks whereas the discriminant function of shape indices only correctly identified 62.8% specimens into right stocks. As the sagittae in L. haematocheilus had an irregu...
Aribah Naz1*, Uzma Badar1,Afsheen Arif2*, Zaid Qureshi1, Sondas Saeed1,Erum Shoeb1, Maqsood Ali Ansari1 and Obaid Yusuf Khan1
...ncing. The sequence data analysis of the isolated strain showed 98% homology with Vibrio harveyi species. The sequence obtained has been submitted to NCBI under GenBank Accession# KY653092. Characterization of luminescent bacteria facilitates understanding of the environmental differences that favors the stability, survival and proliferation of species in their respective habitat.
...
Zengwen Huang1,2, WuReliHazi Hazihan2*, Baheti Bodai2, Kadyken Rizabek3, Nuralieva Ulzhan3, Omarova Karlygash4, Juan Zhang1 and Yaling Gu1*
...ly 40 KDa protein. Q-PCR analysis showed that the plasmid pGenesil 10-3p-siRNA could interfere with the expression of INHα in granulosa cells to an efficiency of 83%, which was also confirmed through western Blot assay.This study successfully constructed the eukaryotic expression plasmid pGenesil 10-3p-siRNA, and confirmed that the plasmid interfered significantly with the expression of INHα gene in YM sheep. Our current findings can serve as a mod...
Yan Zhou1,2, Hai Xia Han1,2, Qiu Xia Lei1,2, Jin Bo Gao1,2, Wei Liu1,2, Fu Wei Li1,2, Jie Liu1,2 and Ding Guo Cao1,2*
... 91.75%. The correlation analysis showed that diplotypes (H1H1, H2H2 and H3H3) were significantly associated with egg production at age of 40W (E40) (P=0.0342) and egg production at age of 43W (E43) (P=0.0184). The egg production at age of 38W (E38), E40 and E43 of H2H2 chickens were all higher than those of the H1H1 and H3H3 chickens. Compared with H1H1 and H3H3 chickens, the highest mRNA levels of VLDLR were found in ovary, 6 mm - 8 mm follicle...

Muhammad Ismail1, Tufail Ahmad2, Shamsher Ali2*, Shahid Ali1, Nur Ul Haq1 and Naveedullah3

Heavy Metals (Pb and Ni) Pollution as Affected by the Brick Kilns Emissions
... were also collected for analysis of heavy metals in available locations around the chimneys in 16 plastic buckets. They were placed 3 m high from ground. The laboratory analysis of wheat leaf samples showed that the concentration of  Pb and Ni in chimney A and B as 2.90 and 1.8, 13.11 and 14.7 mg kg-1 respectively. The concentration of Pb and Ni at this stage is not in toxic range however with the continuous addition m...
Ishrat Perveen1, Yasar Saleem2 and Javed Iqbal Qazi3* 
...V detector. Quantitative analysis of the control samples (only thawed at room temperature) revealed presence of HCAs in K, S, D and B brands with respective values of 0.97, 1.09, 5.63 and 13.66 µg/g. The high thermal cookings of the RTE samples enhanced the levels of HCAs. Microwave cooking was found to produce highest total HCAs contents (0.71-35 µg/g) followed by deep-frying (1.62-22.21 µg/g) and pan-frying (1.11-8.57 µg/g) of the RTE...

Khalil Ahmed1*, Amar Iqbal Saqib1, Abdul Rasul Naseem1, Ghulam Qadir1, Muhammad Qaisar Nawaz1, Muhammad Khalid2, Imtiaz Ahmad Warraich3 and Muhammad Arif4

Use of Hyacinth Compost in Salt-Affected Soils
...wn in the rotation. Data analysis showed that gypsum and hyacinth compost remarkably improved the soil SAR, pHs, ECe, BD, HC, growth and yield characteristics of rice and wheat crops, however at the same time use of gypsum and hyacinth compost in combination proved more superior to their sole application. Hyacinth compost @ 10 and 15 t ha-1 with gypsum @ 50 % of GR performed equally in all studied parameters of rice and wheat crops and soil properties. Therefo...

Olugbenga Omotayo Alabi1*, Ayoola Olugbenga Oladele2 and Ibrahim Maharazu3

Profitability Analysis and Marketing Efficiency of Soyabean (Glycine max) Value Chain among Actors in Abuja, Nigeria
...statistics, gross margin analysis, financial analysis, marketing margin, marketing efficiency, Probit model analysis, five point Likert scale and principal component analysis. The results revealed that most (86.33%) of the soyabean marketers were between the ages of 31-60 years, about 83.33% of soyabean marketers had less than 5 years marketing experienc...

Abid Khan*, Mukhtar Alam, Yousaf Jamal and Hidayat Ullah

Appraisal of Yield and Yield Components of Wheat with Integrated Management of Nitrogen Sources
...omputed according to lab analysis from each combination except N1 and incorporated once after final seed bed preparation each year. Based on mean data over two consecutive years, N6 resulted significantly maximum spike length (9.8 cm), spikes m-2 (303), grains spike-1 (43.1), 1000 grain weight (34.71 g) and grain yield (3917 kg ha-1) showing an increase of 122.2% in grain yield over the control. Significant improvements in above-mentioned parameters were obser...

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
... Primers based diversity analysis indicates high polymorphism within the collected wilt isolates. Primers ITS 1 and ITS 4 generated internal transcribed spacer fragments that were restricted four restriction enzymes (HhaeI, MboI, BsuRI, HinfI). PCR ITS-RFLP banding pattern identified two Fusarium species i.e. Fusarium oxysporum and Fusarium solani. In conclusion, PCR-ITS-RFLP proved to be an efficient and easy technique for the identification of wilt complex p...

Rana Shahzad Noor1,2, Fiaz Hussain2, Irfan Abbas3, Muhammad Umair2, Abu Saad2, Muhammad Umar Farooq4 and Yong Sun1*

Assessing Sustainability of Rainfed Wheat (Triticum Aestivum) Production under Various Soil Tillage Systems: An Energy and Economic Analysis
...luated in term of energy analysis and economics of wheat production. Highest energy inputs obtained were fertilizer, seed and fuel + oil energies in all tillage systems for wheat sowing, respectively. Results interpretation indicated that the highest (2,300 kgha-1 and 40.56 lha-1) and lowest (1,930 kgha-1 and 8.97 lha-1) wheat yields were measured in CT and ZT farming treatments, respectively. The maximum and lowest energy values were calculated in PT (6.03) a...
Sehrish Firyal1,*, Ali Raza Awan1, Muhammad Umair Latif1, Muhammad Tayyab1, Muhammad Wasim1, Shagufta Saeed1 and Imaad Rashid2

 

...tide polymorphism (SNPs) analysis is a powerful tool for taxonomic and phylogenetic analysis. These are bi-allelic molecular markers, easy to interpret and uniformly distributed within genomes. In this study cytochrome b (Cytb) gene based SNPs analysis was employed to genetically characterize the Pakistani domestic breed Lathy pigeon. Cytb haplotype for local domestic breed w...

Paul Oludare Adetunji* and Safiriyu Idowu Ola

Comparative Productive Performance among three Plumage Varieties of Noiler Hens
... to Spearman correlation analysis. Plumage colour difference had a significant (P<0.05) effect on the onset of puberty as lower age at first egg were recorded in brown and black pullets compared to the barred pullets. Black hens had the best performance in most of the egg production indices with the values of 61.57±0.56g, 68.38±2.49%, 157±3.55 eggs, 2.39±0.05, NGN266.56±8.31 and 12 weeks (at 85.37%) for egg weight at 52nd ...

Rao Wali Muhammad1, Hafiz Muhammad Wasif Ali2*, Amir Hamza1, Muhammad Qadir Ahmad2, Abdul Qayyum2, Waqas Malik2 and Etrat Noor2

Estimation of Different Genetic Parameters in Various Safflower (Carthamus tinctorius L.) Genotypes under Field Condition
... and principal component analysis were carried out to estimate the extent of variability for studied parameters and to partition the germplasm into various cluster groups on basis of their mean performance. A value of 74% was indicated by the first three PCs, which showed the highest variability between studied parameters. Eigen values >1 contributed variability between the genotypes under field condition. Minimum and maximum heritability estimates ranged b...

Muhammad Arsaln1, Sair Sarwar2*, Rizwan Latif1, Junaid Nawaz Chauhdary3, Munazza Yousra2 and Shahbaz Ahmad2

Effect of Vermicompost and Microbial Inoculants on Yield, Soil Fertility and Economics of Wheat under Rainfed Conditions
...) contents. The economic analysis revealed that maximum net returns were found in integrated nutrient application (RDF, VC and microbial inoculants) i.e., Rs. 67,044 ha-1 for 2017-18 and Rs. 1,03,202 ha-1 for 2nd year and minimum returns in control.
...

Attiq ur Rehman1,2*, Iftikhar Hussain Khalil3 and Ihtisham Ali4

Genetic Diversity and Traits Association in Tetraploid and Hexaploid Wheat Genotypes in Khyber Pakhtunkhwa Province of Pakistan
...t genotypes. Correlation analysis revealed that tillers m-2 and spikes m-2 had significantly positive association with grain yield while grain yield had significantly strong positive association with harvest index in tested germplasm. Durum genotypes DWE3 and DWE7 performed best for yield contributing traits while spring wheat verities, Janbaz, Barsat and Shahkar outperformed others in terms of 1000-grain weight, grain yield, and harvest index, respectively. T...

Shahzad Khan*, Munir Khan, Inayatullah Jan, Mahfooz Khan and Fida Muhammad Khan

Determinants of Sugarcane Yield in District Charsadda, Pakistan
...d to perform an economic analysis and to investigate determinants of sugarcane crop in district Charsadda in the year 2018. Three villages viz. Kamran Kalay, Aspandehri, and Sarfaraz Kalay were selected purposively. Primary data were collected with face to face interview method from 41 randomly selected sugarcane growers through structured questionnaire. The selection of farmers was based on proportional allocation technique in the selected villages. For data ...

Khan Sher1*, Muhammad Subhan1, Muhammad Nisar2, Ali Hazrat2, Zahid Fazal1, Gul Rahim2, Imran Ahmad1, Riaz ul Haq1 and Shamia Bibi1

Genetic Diversity in Common Beans (Phaseolus vulgarus L.) Collected from Different Ecological Zones of Malakand Division (A Part of the Sino Japanese Region of Pakistan)
...ogram in one-way cluster analysis distributed 55 lines into 2 lineages (I and II). The former was comprised of 24% bean landraces, while the latter was comprised of 76% landraces. In correlation coefficients 45 combinations were identified having strong positive correlation. The strongest correlations (above 50%) were computed 67% of all the combinations. The principal component analysis (PCA) identified quantitative variabi...
Aatif Amin1,*, Zakia Latif2, Arslan Sarwar1, Basit Zeshan1 and Mushtaq A. Saleem1
...otyping and phylogenetic analysis were performed for the characterization of isolates as Bacillus sp. AZ-1 (KT270477), B. cereus AZ-2 (KT270478), B. cereus AZ-3 (KT270479) and Enterobacter cloacae ZA-15 (KJ728671). Phylogenetic relationship on the basis of merA nucleotide sequence confirmed 51-100% homology with the corresponding region of the merA gene of already reported mercury resistant Gram positive bacteri...
Oluwatoyin Adenike Fabiyi1*, Olubumi Atolani2 and Gabriel Ademola Olatunji3
Toxicity Effect of Eucalyptus globulus on Pratylenchus spp. of Zea mays
...nd 13C NMR) Spectroscopy analysis. Effect of application of plant phytochemicals on the planted maize was also examined in terms of growth rate and survival of Pratylenchus spp. The essential oil (ECSG/EO) exhibited significant (p<0.05) toxicity, reduced nematode population and increased grain yield (6.79 kg) compared to the standard, carbofuran (CBFN) (7.18 kg) The Eucalyptus globulus ethanol extract fractions (ECSG/EtOH) showed moderate effect, while the ...

Asif Ali Abro1* and Iqbal Ahmed Panhwar2

Impact of Various Factors on Crop Diversification Towards High Value Crops in Pakistan: An Empirical Analysis by using THI
...ained as a result of the analysis indicate a high level of crop concentration and need to be diversified towards high value crops. The ARIMA time series model (1,1,1) was used to study the impact of various factors on the Transformed Herfindahl Index (THI) of diversity. To check the impact of improved infrastructure; the length of the road was adopted and showed a positive and significant relationship with crop diversification. Per capita income, urbanization,...

Mehnaz Safdar and Urooba Pervaiz*

Constraints in Accessing Agricultural Extension Services by Rural Women: Evidence from Khyber Pakhtunkhwa, Pakistan
...to trainings. Chi-square analysis revealed that age had significant association with involvement in decision making. It means that by increasing age of the respondents, the tendency towards involvement in decision-making increases. Literacy had non-significant association with decision making and training attended. Similarly, various barriers were identified having significant impact on the probability to attend trainings. Results indicated that women extensiv...
Rubina Munir1,2*, Muhammad Zia-ur-Rehman3, Muhammad Makshoof Athar1, Noman Javid1,4, Ayesha Roohi2 and Syeda Fatima Razavi2
...established by elemental analysis. Free-radical scavenging activity of these compounds has been carried out by DPPH assay that showed these compounds to be effective radical scavengers. The compounds 7a, 7b, 7d, 7f and 7h have exhibited radical scavenging activity better than the reference compound BHT.
...
Muhammad Umer Khan1,2*, Muhammad Farooq Sabar1, Atif Amin Baig3, Arif-un-Nisa Naqvi4 and Muhammad Usman Ghani1
...ed through computational analysis. A total of 75 different haplotypes were identified in Shin people; among them, 72 were unique and 3 were shared by more than one individual. This study revealed the predominance of West Eurasian lineages in the Shin population (59.49%), followed by South Asian lineages (25.32%) and then East and Southeast Asian lineages (15.19%). Shin population presented a high genetic diversity of 0.9996 and a low random match probability o...
Amir Aziz1*, Mukkram Ali Tahir1, Noor-us-Sabah1, Ghulam Sarwar1 and Sher Muhammad2
Effect of Rice and Wheat Straw and K-Silicate Application on Maize Growth
...of interest. Statistical analysis of collected data was done using Statistics 8.1 software. From the results it was concluded that by the using the both sources of silicon (straw and potassium silicate), plant growth and yield were improved but among these sources, potassium silicate was the best.  Results showed that potassium silicate @ 200 mg kg-1 of soil gave higher values in almost all physiological and yield parameters.

...

Muhammad Siyar1, Anwar Saeed Khan2*, Fazlur Rahman2 and Muhammad Jamal Nasir2

Spatio-temporal Assessment of Reliability in Pehur Main Canal System, Khyber Pakhtunkhwa, Pakistan
...unreliable services. The analysis shows the majority of reliability values closer to 0. Rabi season has better reliability than the Kharif Miana. The spatial reliability in different sections of the system as a whole, distributaries and minors are unpredictable. This situation points towards lack of operational maintenance of the canal system.

...

Malik Abdul Rehman1*, Hafiz Muhammad Ehsan2, Tehseen Ashraf2, Zulfiqar Ali Gurmani3, Sajjad Khan3 and Mujahid Ali2

Effect of Growing Media on Plant Growth of Rough Lemon (Citrus jambhiri Lush.) and Poncirus (Citrus trifoliata)
...e was recorded. Chemical analysis of leaf was done by taking samples from treatments were analyzed for determination of N, P, K. Results indicated that stem length, stem diameter, number of leaves, leaf area and survival percentage were significant in different growing media after everyone months of transplantation. Various media i.e. peatmass+soil+sawdust, leafmanure+soil+sawdust, compost+silt+sawdust, leaf manure+silt+saw dust, compost+soil+sand was proved p...

Syeda Farzana Bibi* and Siraj ud Din

Unraveling the Bioherbicidal Potential, Elemental Analysis and Nutritional Evaluation of Crataeva adansonii Dc Leaf and Bark
...lemental and nutritional analysis of the plant parts was also done to accentuate this plant’s use in the nutritional and agricultural industry. Elemental analysis of C. adansonii stem bark and leaf was assessed through atomic absorption spectrophotometry to detect ten essential macro and microelements, including Na, Ca, Co, Cd, Cu, Mn, Zn, Pb, Ni, and Fe. In the bark part, the highest percentage of relative standard de...
Muhammad Aamir Naseer1, Amjad Islam Aqib2*, Ambreen Ashar3Muhammad Ijaz Saleem1, Muhammad Shoaib4Muhammad Fakhar-e-Alam Kulyar1, Khurram Ashfaq1, Zeeshan Ahmad Bhutta1 and Shagufta Nighat5
...factors, and comparative analysis of antibiogram of bpSA and nbpSA at various cadre. Milk samples (n=250) from cattle (n=90) and buffalo (n=160) were aseptically collected from various dairy farms and put to biofilm detection and antibiogram. Based on collected data with statistical inferences, the study found 61.60% of S. aureus from subclinical samples, while 72.73% of S. aureus were positive for biofilm with uniform hike in samples from cattle...
Ayesha Talib1,2, Khanzadi Nazneen Manzoor1, Wajahat Ali1, Maria Saeed1, Muhammad Asif Gondal1, Malik Badshah3 and Abid Ali Khan1*
...ough UV-Vis spectroscopy analysis. The electron microscopy imaging revealed that the biogenic Cu nanoparticles were spherical in shape and had a diameter of 30-40 nm. The synthesized nanoparticles showed promising antibacterial activity against MDR clinical isolates of bacteria.
...

Kecheng Zhu1,2,3, Peiying He1, Baosuo Liu1,2,3, Huayang Guo1,2,3, Nan Zhang1,2,3, Liang Guo1,2,3, Shigui Jiang1,2,3 and Dianchang Zhang1,2,3,* 

...r cloning, bioinformatic analysis and transcriptional analysis of Acanthopagrus latus myomaker (Almyomaker) were performed. The open reading frame (ORF) sequence of Almyomaker is 858 bp, which encodes a polypeptide of 285 amino acids. Moreover, phylogenetic and gene structure analysis indicates that Almyomaker is highly conserved among vertebrates. The tissue distribut...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
...valuated by western blot analysis. MTT assay revealed that spermidine decreased the viability of Hela cells in a dose-dependent manner in conjunction with disrupted morphology. Moreover, spermidine caused cell damage as evidenced by elevated LDH leakage and compromised MMP as demonstrated by flow cytometry. Concomitantly, western blot analysis showed that spermidine induced autophagy by activating the LC3 conversion and p62 ...

Fan Sigang1, Guo Yihui1* and Xu Youhou2

...the first transcriptomic analysis of C. nobilis, which will help clarify the molecular mechanisms of gonadal maturation.
...
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
...Environmental DNA (eDNA) analysis is a powerful tool within ecology for the distribution or abundance study of aquatic species. DNA extracting was an indispensable process to employ this method. In eDNA extracting of macro-organisms, DNeasy Blood and Tissue Kit was commonly used, but its price was about 80 RMB/sample, which was too expensive. Cheaper eDNA extraction kit was a potential aspect for improvement to expand further application of eDNA
Gautam Patra1*, Ana Sahara2, Sonjoy Kumar Borthakur1, Parthasarathi Behera3, Subhamoy Ghosh1, Apurba Debbarma4 and Seikh Sahanawaz Alam5
...quences and phylogenetic analysis of cyt b gene, 8 isolates of P. relictum were identified. The obtained complete nucleotide sequences of cyt b gene from P. relicum revealed100% identity among themselves while the other sequences registered in Gen Bank showed 95% to 99% similarity. In the phylogenetic analysis all the isolates of P. relictum formed a separate clade with high bootstrap values. It c...
Jian Yang1, Guangxiang Tong2, Zhipeng Sun2, Xianhu Zheng2, Weihua Lv2, Dingchen Cao2, Xiaowen Sun2 and Youyi Kuang2*
Xia Li1*, Xueliang Zhan1 and Xinjie Shi2
.... The arterial blood gas analysis showed that the arterial blood PO2 was elevated and PCO2 was decreased in the study group. The difference was statistically significant (P<0.05). It was concluded that the application of ulinastatin combined with ambroxol in the treatment of AECOPD complicated with type II respiratory failure can significantly improve the treatment effect, help patients actively improve the condition and promote body r...
Jianjun Fu1, Shili Liu2, 3, Wenbin Zhu1, Yongyi Jia2, 3, Lanmei Wang1, Yufen Yang4, Zhimin Gu2, 3*and Zaijie Dong1*
...ased on allele frequency analysis using 36 candidate parents, the number of alleles ranged from 7 to 22, observed heterozygosity ranged from 0.39 to 0.75, and expected heterozygosity ranged from 0.57 to 0.91. All loci were highly informative (polymorphic information content, PIC >0.5). We detected a significant correlation between exclusion probabilities for parent pairs (E-PP) and PIC (P<0.01). Simulation

Masoud Hassani1* and Omid Madadgar2,3

Serological Evidence of Bluetongue in Iran: A Meta-Analysis Study
... of Iran based-on a meta-analysis study. The meta-analysis study was conducted in national and international databases to find articles which evaluated bluetongue seroprevalence by antibody-captured ELISA test in livestock in Iran by searching terms including bluetongue, sheep, ovine, goat, caprine, cow, cattle, bovine, buffalo, camel, Iran and prevalence alone or in combination in both English and Farsi language. After revi...
Abdul Majid Khan1,*, Muhammad Tahir Waseem1, Rana Manzoor Ahmad2, Ayesha Iqbal1, Hafiza Imrana Naz1, Amtur Rafeh1 and Muhammad Ameen1
... teeth. The morphometric analysis of the dental specimens led us to recognize the sample as belonging to Eotragus noyei, which has been considered as the smallest and the oldest bovid in the Siwaliks. Eotragus noyei is characterized by brachydont teeth, finely rugose enamel, more inclined buccal walls of the molars and small lingual cingula. The inclination of the metaconal area has caused rotation of the metastyle in relation to the antero-poste...
Muhammad Mohsin1, Yin Hengbin2,* and Zhang Luyao3
...., catch and effort data analysis (CEDA) and a stock production model incorporating covariates (ASPIC). For initial proportion (IP) 1, CEDA estimated MSY in a range between 41000-50000 t, whereas, ASPIC calculations remained between 44000-52000 t. Furthermore, results also showed that fishing mortality (F) is increasing while biomass (B) is swiftly decreasing. Based on the results it can be concluded that Spanish mackerel is facing the risk of overexploitation...
Inga Kowalewska-Łuczak1 and Ewa Czerniawska-Piątkowska2,*
...;"> - 0.843. Statistical analysis allowed to show, in the case of SAA2 c.-84 G>C polymorphisms, cows with the CC genotype were characterized by the highest content of protein and the lowest content of fat in milk. On the other hand, for the
Xuya Zhou1, Ying Liu1, Deqin Xu1, Jie Bao1, Yaru Cao2 and Yong Jin3*
...sure meter. Western blot analysis showed that unification of two drugs caused protein expression levels of COX-2 to reduce obviously and increased protein expression levels of CYP4A1. Immunohistochemistry showed the increased expression of CYP4A1. Elisa indicated that the decreased production of PGE-2 and the increased production of 20-HETE, ET-1 and BNP in blood serum. The blood pressure of rats was increased. The results indicated that unification of two dru...

Muhammad Jamal Nasir*, Atiq Ur Rahman and Anwar Saeed Khan

Assessment of Irrigation Development in the Small Dams Command Area: A Case Study of Palai Dam, District Charsadda
...The study is based on an analysis of revenue data acquired from revenue office Tangi, between 2008-09 to 2016-17. The geospatial analysis of collected data is done using the software ArcGIS 10.5.2. The analysis revealed a tremendous increase in canal irrigated area. Before construction of the dam in 2008-09, the canal irrigated area in Palai Nasratzai was 138 acres (5.39% of the total cult...
Inam Ur Rahman1, Syed Faizan Iftikhar2, Sanam Wagma Khattak3*, Wajahat Ali1, Azra4 and Sajad Ali1
South Asian Experience of Trade Liberalization: Implications for Food Security
...onomies. The descriptive analysis revealed that Pakistan is the most and Nepal is the least food secure country in the region. Moreover, at country level the food availability and accessibility has lower values, where most of the countries are below the regional average values. While exploring the implications of trade liberalization for food security this study uses the panel data of five south Asian economies for a period of 24 years by utilizing the fixed e...

Asifa Kamal1*, Ghazala Noreen1 and Muhammad Amin2

Trends and Determinants of Fertility in Pakistan: Multivariate Decomposition Analysis (PDHS 1990-91 to PDHS 2012-13)
...ltivariate decomposition analysis and pooled cross section techniques are used to analyse the data. It is concluded from multivariate pooled analysis of three surveys that age of women at first birth, region (Balochistan), wealth index (rich), education of both spouses and survey periods (2006-07, 2012-13) has significantly negative effect on the total children ever born to women in Pakistan. Child mortality (yes), husband&r...
Yifan Ni1, Jian Liu1, Fen Wu1, Jianfeng Cai1, Jinzhi Zhang1*, Jianqing Hua2 and Jiping Fu2
...rr;Gln). The association analysis indicated that the GG genotype of MDR1 gene and the TT genotype of OPN gene had the highest values for the total number of piglets born (TNB), the number of piglets born alive (NBA), piglet weight at birth (PWB) and litter weight at birth (LWB) in both primiparous and multiparous sows of the three breeds. These results suggested that the SNP locis of MDR1 and OPN genes can be used in Marker-Assisted...
Saima Siddique1* and Zarrien Ayub2
...h-frequency distribution analysis was bimodal in different seasons with the exception of three modes in summer’2012 and one mode in autumn’2012. Estimated parameters of the von Bertalanffy growth functions include growth coeffient (K) = 0.48 year-1, theoretical asymptotic maximum size (TD∞) = 8.2 cm and hypothetical age at length zero (t0)= -0.55 year. The longevity was calculated to be 5.7 years. The natur...
Anjali Khadka1*, Subodh Raj Pandey2, Subarna Sharma Acharya3, Amrit Poudel4 and Sushma Adhikari1
Morphological Evaluation and Multivariate Analysis of Soybean Glycine max (L.) Merrill Genotypes in Western Mid-Hills of Nepal
...th grain yield. The path analysis depicts that the internodal length and days to maturity had the highest direct positive and negative effect on yield respectively. Though test weight had a significant positive correlation with grain yield, it had a negative direct effect on grain yield. The cluster analysis grouped sixteen genotypes into four clusters among which cluster I was largest with eight genotypes.

...
Abdul Hameed1*, Ihtsham ul Haq Padda2 and Abdul Salam3
Estimating Food Consumption Patterns in Pakistan by Using Ideal Demand System
...ural areas. The regional analysis indicates that the gross and net substitute impacts in urban areas are somewhat more dominant than in rural areas. It implies that urban areas household makes more substitutes relative to rural areas. These findings suggest that policy makers and public organizations should reexamine the connection between consumption, price and income for food and nutrition security and social development. It also proposes that constant price...

Muhammad Ali Shah, Faiz Ur Rehman, Abbas Mehmood, Fareed Ullah, Sayed Irfan Shah and Syed Majid Rasheed*

Combining Ability, Heritability and Gene Action Assessment in Rapeseed (Brassica napus L.) for Yield and Yield Attributes
...ecific combining ability analysis revealed non-significant results for the traits under consideration, suggesting the involvement of non-additive type of gene action. A higher broad sense heritability estimate was evident. It was concluded from the combining ability analysis of the present study that genotype 2702 was best general as well as a specific combiner for pod length (PL), seeds pod-1 (SPP), total yield (TY), and 10...

Sajid Ali1, Abdul Basit1,3*, Abdul Mateen Khattak1, Syed Tanveer Shah1, Izhar Ullah2, Noor Alam Khan3, Imran Ahmad1, Kamran Rauf1, Salman Khan1, Irfan Ullah1 and Intizar Ahmad1

Managing the Growth and Flower Production of Zinnia (Zinnia elegans) through Benzyle Amino Purine (BAP) Application and Pinching
...awar, Pakistan. Research analysis showed that zinnia plants treated with 100mg.L-1 and pinched at 6 leaves stage recorded maximum days to flowering, flower persistency, branches, leaves and flowers plant-1 and stem thickness. While in case of untreated and un-pinched plants maximum flower diameter, fresh and dry flower weight, and height of plant were reported. Hence, it is concluded form the research study that BAP application (@100 mg.L-1) reduced height of ...

Ali Zohaib1*, Habib Ullah2, Shakeel Ahmad Anjum2, Tahira Tabassum2, Muzzammil Hussain1, Mohsin Nawaz3, Ghulam Abbas4 and Sohail Irshad5

Effect of NPK Fertilization Strategies on Growth, Yield, Nutrient Use Efficiency and Economic Benefits of Relay Intercropped Wheat in Cotton
...yield and NUEA. Economic analysis also manifested that the greatest net returns and benefit cost ratio (BCR) were obtained by same NPK application timing (i.e. T4). In conclusion, fertilization timing substantially affected the yield, NUEA and economic benefits of relay intercropped wheat in cotton; and application of ½ N and all PK at sowing and ½ N at tillering was most appropriate option.

...

Imran Ahmad Rana*, Muhammad Ali Yab and Ch. Abdul Rehman

Quality Engineering Tools Implementation a Comparison of Indian and Pakistani Business Organizations
...obtained from regression analysis display that there are also differences between the critical success factors (CSFs) of six sigma implementation in Indian and Pakistani organizations. Elements like management commitment and six sigma deployment strategies were found significant in explaining variability of six sigma results in the region. Results show that the countries were found similar on effect of six sigma practices on customer satisfaction, where common...

Muhammad Kashif Khan1*, Muhammad Wasim Jan Khan2, Kaleem Ullah Jan Khan3, Muhammad Ishaq Khan4, Muhammad Faheem Jan Khan5 and Abdul Salam Lodhi6

 

Impacts of Construction Risk Attributes on Organizational Performance of Registered Construction Companies in Khyber Pakhtunkhwa, Pakistan
... on risk identification, analysis, and mitigation through various methods and techniques. The purpose of this study is to address the need of risk management practices in the construction industry of Khyber Pakhtunkhwa (KPK), Pakistan. The research work carried out with the involvement of 150 Pakistan Engineering Council (PEC) registered construction companies not only emphasizes on the crucial risk factors associated with construction projects but also insigh...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...ion, and in the subgroup analysis of Asian and Caucasian populations. The results showed that in overall population, IL-6 -572 G/C shows a significant association towards increased risk of RA in allelic (OR=0.8130, 95%CI=0.708-0.932, p=0.003), co-dominant and dominant models; same hold true for the Asian population. Stratification by ethnicity revealed that in Asian population also, the association with RA manifestation is significant. However, in a cohort of ...
Zhilong Tian1, 2, Yuqin Wang2, Jishun Tang1,3 and Mingxing Chu1*
...nges. Population genetic analysis indicated that SYNE2 gene g.73310578G>A, g.73312791A>G showed moderate polymorphism (0.25<PIC<0.5) in Small Tail Han sheep. Furthermore, g.73310578G>A and g.73312791A>G loci were closely linked in STH (r2 > 0.33). Association analysis results showed that g.73310578G>A and g.73312791A>G SNPs significantly affected litter size (P < 0.05). ...
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*

Muhammad Nawaz Kandhro1*, Zulfiqar Ali Abbasi1, Aijaz Ahmed Soomro1, Naimatullah Leghari2, Muhammad Ibrahim Keerio3, Ahmed Naqi Shah1, Ghulam Murtaza Jamro4, Nadia Mangrio1 and Shazia Parveen Tunio5

Evaluation of Irrigation Frequencies and Seed Priming with Plant Nutrients on Growth and Yield of Sunflower (Helianthus annuus L.) Genotypes
...O-1) and (Hysun-33). The analysis of data indicated that irrigation frequencies, seed priming and their interaction affected significantly (P≤ 0.05) on growth and yields of traits of sunflower genotypes. In case of irrigation frequencies, enhanced seed yield (2256 kg ha-1) was recorded in five irrigations followed by four irrigations having non-significant differences with each other. Genotype, HO-1 was found superior over Hysun-33 by producing greatest see...

Zohaib Ahmad Hassan1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Usman Saleem3, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz4, Khurshid Ahmad Mufti5 and Aamer Sattar2

Toxic Effects of Sodium Bicarbonate (NaHCO3) on Growth and Yield of Rice
... experiment. Statistical analysis of all collected data was accomplished. It was noted from the results that control (T1) proved superior with regard to plant height, fertile tillers/pot, total biomass, straw and paddy yield. All these yield contributing components indicated a declining trend in all subsequent treatment. Treatment T7 (40 SAR) proved inferior to all others in these regards.

...
Arnab Tanveer, Shabana Naz*, Azhar Rafique and Asma Ashraf
...itative and quantitative analysis. Direct smear method and fecal floatation technique was used for isolation and identification of endo-parasites. Modified McMaster technique was used to calculate Eggs per gram. The data was subjected to ANOVA and Tukey-HSD (post hoc test). Six species of endo-parasites were identified from Jallo Wildlife Park. Eimeria (7500) and Ascaris (1100) were the most abundant. Murree Wildlife park had only two species i.e...
Ehsan Kashani1, Hamid Reza Rezaei2*, Morteza Naderi3 and Nematolah Khorasani4

 

...g the past. Phylogenetic analysis successfully grouped the specimens in two distinct clades without any common haplotypes. Long-eared specimens grouped in two distinct phylogenetic groups which belong to two different geographic areas while for P. hypomelas no significant divergence happened in the species life history.
...

Zaka ur Rehman1*, Kashif Akbar1, Altaf ur Rehman1, Hassan Waqas1, Alif Khan1 and Asad Ali2

Development and Storage Stability of Diet Mango Juice
...ture for physicochemical analysis (Total soluble solids, ascorbic acid, pH, percent acidity, and sugar-acid ratio) and organoleptic evaluation (appearance, taste, flavor, overall acceptability) for three months. These were prepared with sweeteners, numbered as (T0) pure mango juice (control sample), (T1) mango juice+2.5g sucralose, (T2) mango juice+2.5g cyclamate, (T3) mango juice+2.5g aspartame, (T4) mango juice+1.25g sucralose + 1.25g cyclamate, (T5) mango j...

Ambreen Akhtar Saddozai1, Amer Mumtaz2, Naseem Rauf1, Saeeda Raza2, Nouman Rashid Siddiqui2, Muhammad Naeem Safdar2, Sahar Shibli2, Muhammad Suhail Ibrahim3*, Muhammad Akhtar4 and Muhammad Saad Rehan5

Preparation and Quality Evaluation of Soymilk Carrot Blend
...ntents and antimicrobial analysis. On the basis of quality attributes it was observed that plain and carrot supplemented soymilks remained acceptable up to 7 days at refrigerated temperature. Plain soymilk had lower TPC at both temperatures than carrot supplemented milk. pH, TSS, carotenoids and protein contents in samples were decreased with increased time at both the temperatures. Highest protein and carotenoid content was detected in 6% carrot supplemented ...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Sher Muhammad3, Usman Saleem4, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz5 and Aamer Sattar2

Impact of NaCl Toxicity on Yield and Yield Components of Rice (Oryza sativa) Grown in Aridisol
...ried out and statistical analysis was performed. It was observed from the results that addition of NaCl proved toxic for growth and yield of rice plants as well as associated parameters such as plant height, number of productive tillers, total biomass, straw and grain yields were negatively influenced by the increasing levels of sodium chloride when compared with control treatment. Injurious impact of NaCl was more pronounced as the concentration of salt incre...

Zaheer Ahmed Deho*, Saifullah Abro and Muhammad Rizwan

Assessment of Stability for Seed Cotton Yield of Cotton Genotypes Across Different Environmental Conditions of Sindh Province
...h location and stability analysis (genotype x environment interaction) was performed. The pooled analysis of variance for seed cotton yield, showed highly significant (P< 0.05) differences for varieties, locations and varieties × location interaction. The analysis depicted the significance of both the linear and non-linear components which showed the presence of individually expec...

Muhammad Rizwan1*, Jehanzeb Farooq1, Muhammad Farooq1,2, Aqeel Sarwar4, Abid Ali3, Farrukh Ilahi1, Muhammad Asif1 and Ghulam Sarwar1

Quantitative Studies in Upland Cotton (Gossypium hirsutum L.) using Multivariate Techniques
...g). Using Mahalanobis D2 analysis, these parameters were assembled into seven clusters. Among these clusters, cluster I and VII were largest each having nine and eight genotypes respectively, followed by cluster VI having seven genotypes. According to the illustrations by using hierarchical cluster analysis, total genotypes were grouped in seven clusters with 11 genotypes in cluster VI after that cluster II comprising 9 geno...
Ahmad Sadiq1, Muzafar Shah2*, Habib Ullah1, Irfan Ali1, Amir Alam3
Navid Jalil1 and Muhammad Khan3
...or control. Gender-based analysis has shown that HbA1c, RBS, DBP and SBP in male patients have significantly higher (p<0.05) than female. But in female patients the TC, TG and BMI are insignificantly higher (p<0.05) compared to male. High density lipoprotein (HDL) was found same in both male and female. Moreover, patients with HbA1c > 7 have 26.66% stroke, 4.44% angina, 2.22% ischemic heart disease and 27.77% micro vascular complications.<...
Wentao Wang1,2, Xu Lin3, Jianshu Zhuo3, Dongjie Zhang2, Xiuqin Yang3* and Di Liu1, 2*
...in combination. Minigene analysis showed that the splicing of porcine E2F3b is complicated. E2F3b isoforms are expressed in all tissues studied with high level in spleen and muscle. Both of isoforms V1 and 2, containing functional domains of E2Fs, were localized throughout cells. No functional nuclear localization sequence and export signal were characterized through site-directed mutagenesis analysis, although their existen...
Nadia Islam1, Khalil Ahmed1,*, Maisoor Ahmed Nafees1, Mujtaba Khalil2, Ishtiaq Hussain3, Muhammad Ali1 and Raja Imran1
Zhen-Yang Wu1, Li Li1, Yu-Hua Fu2, Sheng Wang3, Qing-Ming An1, Xiao-Hui Tang4, Xiao-Yong Du3,5,* and Fei Zhou6,*
... sequencing. The RNA-Seq analysis generated 63,283,784 and 63,644,062 clean reads in black and white skin, respectively. A total of 60 differentially expressed genes (DEGs) were identified, providing evidence that the different coat color skin changed considerably. Among these differentially expressed genes, there were 18 up-regulated genes and 42 down-regulated genes in black skin tissue. Further analysis showed that these ...
Ayesha Alam1,* and Labeeb Ali2
...inging attention towards analysis of sewage water. Based on information from cited literature it is estimated that an individual handling sewage water have approximately 1% chance of becoming infected with virus. Treated sewage effluent and reuse of sewage water for recycle purposes must be considered as it may lead to widespread of COVID 19 in coming decade. Thus, a qualitative risk assessment and disinfection of water supplies based on reported infection rat...
Jun Yan Bai*, Zhi Hao Dong, Hui Rong Gong, Xiao Ning Lu and Zi Heng Li
Aamer Sharif1,*, Muftooh Ur Rehman Siddiqi1 and Riaz Muhammad2
...tudy is the experimental analysis of novel runner which has the ability to maximize output power and efficiency. Computational fluid dynamic (CFD) is carried out on novel runner and ANSYS (CFX) was used to numerically analyze the water flow over the channel and formed water vortex in a conical basin. The experimental analysis was absorbed by measuring the rotational speed (rpm), torque, output power and efficiency of the vor...
Sana Mehmood1, Hafiz Muhammad Tahir1*, Muhammad Summer1, Sher Muhammad Sherawat2, Shaukat Ali1*, Sajida Naseem3
...was confirmed by barcode analysis of tissue samples. A standard barcode sequence of COI (Cytochrome c Oxidase I) was recovered from 90 specimens of spiders. Percentage accuracy of morphological identification was 92.10%. Four morphologically misidentified spiders were allotted to correct taxon after molecular identification. To describe the species diversity and richness in numerical structure, diversity indices (Shannon’s and Simpson’s) and evenne...
Vijay Lal and Muhammad Naeem*
...s been done on proximate analysis of the fish around the world but very little analysis on marine fish Terapon jarbua have been performed. Therefore, the current analysis was carried out to analyze the proximate composition of T. jarbua. 70 samples of T. jarbua ranging from 18.00-29.90 cm in length and 119.60-429.50 g in body weight were collected from Fisheries Harbou...

Muhammad Riaz Gondal1*, Sultan Ahmad Rizvi1, Aaqib Riaz2, Waqas Naseem1, Ghulam Muhammad1, Mazher Iqbal3, Humara Umer3 and Inam ul Haq3

Apposite Sowing Techniques to Optimize Productivity and Profitability of Berseem
...cing of 30 cm). Economic analysis indicated a maximum benefit-cast ratio of 3.51 under broadcast sowing in standing water as compared to control.
...

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz3, Abid Niaz2, Khurshid Ahmad Mufti4 and Muhammad Arif2

Deleterious Impact of Sodium Chloride (NaCl) Toxicity on Chemical Properties of Soil and Ionic Composition of Rice
...om each pot for chemical analysis (Na and K). Soil samples were also collected from all treatment pots and subjected to laboratory for determination of various chemical properties (pH, EC and SAR). To calculate SAR values of soil, concentration of Ca2+, Mg2+ and Na+ were also determined from all soil samples. It was observed from the results that addition of NaCl proved toxic for different chemical properties of soil as values of pH (7.23 to 8.44), EC (2.96 to...

Saba Aleem1*, Mehvish Tahir2, Iram Sharif3, Muqadas Aleem4, Muhammad Najeebullah2, Ali Nawaz1, Amina Batool1, Muhammad Imran Khan1 and Waheed Arshad1

Principal Component and Cluster Analyses as Tools in the Assessment of Genetic Diversity for Late Season Cauliflower Genotypes
... and principal component analysis measures the amount of genetic diversity in respect of several charaters and asseses the relative contribution of different traits to the total variation. In the present study, nineteen genotypes were evaluated for presence of genetic diversity through principal component and cluster analyses. The experiment was designed in randomized complete block design with two replications at the experimental area of Vegetable Research In...

Hafiz Hammad Ahmad1, Mukkram Ali Tahir1*, Noor-us-Sabah1, Ghulam Sarwar1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1 and Aneela Riaz4

Growth and Yield Response of Sunflower to Organic Amendments in Aridisol
...l software Statistix 8.1 analysis of variance technique and significant of treatments was tested using LSD test at probability level of 5%. The maximum fresh biomass (95.50 g), dry biomass (25.73 g), plant height (110.90 cm), head size (21.36 cm), number of achene haed-1 (864) and achene yield plant-1 (32.16 g) of sunflower was observed with application of NK + Rock phosphate at 7 g pot-1 + Wheat straw at 28 g pot-1 (T6) in cultivar Hysun-33. While, lowest val...
Muhammad Umar1, Muhammad Arshad2, Mubashar Hussain1*, Moazama Batool3 and Muhammad Faheem Malik1
...ultaneously hydrological analysis of surface water of Mangla wetland was also conducted. Bird census was carried out in the months of January and February for both years (2010 and 2011) when a large number of migratory birds visit the Mangla Wetland. The birds were counted by the Point Count Method from a fixed point and for a fixed time period in such a way that the Sun was always behind the observer. A total of 37,082 birds belonging to eight orders, 11 fami...
Ghulam Sarwar1, Amna Nazir1*, Muhammad Rizwan1, Eram Shahzadi2 and Abid Mahmood3
...ion, principal component analysis (PCA) and cluster analyses were performed to estimate genetic diversity in 25 cotton genotypes. Correlation analysis revealed that days to first square, flower and boll opening were positively associated with seed cotton yield. Staple length and CLCuV % were negatively associated with fiber fineness and yield respectively. In principal component analysis, ...
Imran Haider1, Muhammad Aown Sammar Raza1*, Rashid Iqbal1, Salman Ahmad1,2, Muhammad Usman Aslam1, Muhammad Israr3, Umair Riaz4, Maliha Sarfraz5, Nasir Abbas6, Salman Haidar Abbasi7, Zafar Abbas8 and Muhammad Aamer9
 
 
...grams were used for data analysis. Drought spell significantly reduced the growth development and wheat yield as compared to control treatment. Application of both growth enhancer amino acid and yeast significantly mitigated the adversarial effect of drought on the wheat as compared to the treatments in which drought was imposed without the application of yeast and amino acid. However, yeast more effectively moderated drought effects than amino acids. Maximum ...

Mansoor Qadir1*, Yousaf Khan2, Shahid Khan3, Djamaleddine Djeldjli3 and Muhanned Ismael Ibrahim Al Firas4

...f Gaussian Approximation analysis and simulation through OptiSystem. It is observed that the proposed 2D-DZ based OCDMA system supports data rate of up to 2 Gbps for relatively large number of subscribers and extended reach in comparison with the existing counterparts. 
...
Nasir Rashid1,2*, Javaid Iqbal1,2, Umar Shahbaz Khan1,2, Mohsin Islam Tiwana1,2 and Amir Hamza1,2
...hnique based on Cepstral analysis is applied primarily in area of speech recognition, mechanical parts analysis, seismological issues etc. Efficacy of EEG brain signal classification, using cepstral analysis-based features is discovered in this study. Mel-Frequency Cepstral Coefficients (MFCCs) is employed as a technique of extracting features from EEG signals. Features classification is p...
Abdul Nabi Jatt1,2*
...es was achieved by GC-MS analysis. Intriguingly, GC-MS technique showed the presence of five different AHL-signal molecules (e.g., C-6, C-8, C-10, C-12, and C-14) produced by C. freundii B1. Moreover, extracellular cellulase enzyme (exoglucanase and endoglucanase) activity wa...
Jia Shi
...stematic review and meta-analysis, a review of articles evaluating response rate to Peginterferon alfa-2a was done through the databases of Scopus, Google Scholar, Pubmed (inception - 2019). Fourteen studies reporting the response rate of HBeAg negative chronic patients’ response to Peginterferon alfa-2a were included in the study. Cochran test was used to determine the homogeneity of the samples (based on Q test and I

 Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*

...=298). Genetic diversity analysis showed that g.1500G>A and g.1694T>A belonged to an intermediate level of genetic diversity (0.25<PIC<0.5), and SNP g.2213C>G belonged to a low polymorphism level (PIC<0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T>A and g.2213C>G had a strong linkage (r2>0.33), a total of four different haplotypes were constructed and the frequency o...
Muhammad Hamayoon Khan*, Niaz Hussain Khuhro, Muhammad Awais, Muhammad Usman Asif and Raza Muhammad
 
 
...> abundance. Correlation analysis of B. dorsalis catches with respect to meteorological data revealed a significantly positive correlation of monthly captured flies with all the climatic factors such as maximum temperature, minimum temperature, R.H. and sunshine duration except the mean monthly rainfall.
...
Muhammad Zubair1, Habib Ahmad2*, Brian E. Hemphill3, Muhammad Tariq4 and Muzafar Shah5
...neighbor-joining cluster analysis and multidimensional scaling. The results remained highly consistent throughout different data reduction techniques. The consistency in phenetic affinities was even evident when they were considered in the backdrop of living ethnic groups of peninsular Indians and prehistoric inhabitants of the Indus Valley and South-Central Asia. When consideration was limited to the people of Northern Pakistan, the uniqueness of Khalil and D...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
... Based on the sequencing analysis results of 46 samples, it showed that new mutation at g.1626G>T was only detected in one individual sample. Polymorphism of bLHR/HhaI gene in the present study was not significantly associated with S/C of Pasundan cows, since the genotype TT of the studied animal was found in the lowest of S/C value than other genotype.
...
Guofang Wu1,2, Xingxing Xue1, Wenjuan Shen1, Lei Wang1,2*, Yuhong Ma1 and Jiping Zhou1
...C18. Further association analysis revealed that the L1-(GT)n locus was significantly associated with teat number (p < 0.05) and that the 152-bp allele exhibited the most positive association. Our data indicate that the L1-(GT)n microsatellite could represent a potential DNA marker with which to increase the number of teats. Thus could help to improve production in the pig breeding industry.
...

Muhammad Jahanzaib1*, Nazakat Nawaz1, Muhammad Arshad1, Haris Khurshid1, Muzamil Hussain2 and Shahid Ali Khan1

 

 

...on, and path coefficient analysis in eleven groundnut advanced breeding lines together with check vis BARD-479, using randomized complete block design with three replications at National Agricultural Research Centre, Islamabad, Pakistan. Through One-Way Analysis of variance significant discrepancies were found among the genotypes for most of the traits. In this study 100 kernels weight was found in significant positiv...
Laura Alejandra Arriola-Mosqueda1, Yadira Jiménez-Lara2, Eugenia del Carmen Prieto-Avella1, Carlos Ricardo Cruz-Vazquez3, Roberto Montesinos-Matias4, Mauricio Valencia-Posadas2, Ana Martha Cruz-Avalos5, Jaime Molina-Ochoa6,7 and César Andrés Angel-Sahagún2,*
...was determined by Probit analysis, and a chi-square test was used to determine the relationship of LC50 with variable radial growth and germination at 18 and 24 h. Germination at 18 h and 24 h ranged from 83.8–92.5% and 89.5–100%, respectively. The average radial growth ranged from 2.3–3.3 mm per day. The pathogenicity results for eggs and larvae showed 100% mortality for both biological stages. The Ma4 strain showed an outstanding...
Iftikhar Hussain1, Sardar Azhar Mehmood1, Muzafar Shah2*, Mohammad Salim3, Shabir Ahmed1, Sana Ullah1,Sidra Batool4, Nadia Saeed1 and Khalid Usman1
...il 7.35 mm. Phylogenetic analysis was performed on the bases of CO1 gene sequences. The mean intraspecific divergences of C. sphinx were 0.011%. The nucleotide composition of the sequences showed higher concentration of A+T as compared to G+C. The A+T contents were 53.1% and C+G were 46.8%. In phylogenetic tree the sequences of C. sphinx clustered together with 100% bootstrap support. The relationship between C. sph...
Rao Muhammad Ramiz1, Arfan Ahmad2*, Aamir Ghafoor2, Muhammad Avais3, Qurat-ul-Ain1 and Muhammad Zahid Iqbal3
...mals (30%). Phylogenetic analysis revealed the circulation of BLV genotype 1 in exotic breeds of cattle. Evaluation of various risk factors analysis demonstrated the strong positive association of BLV with age, herd size, parity, breeding (AI, Bull) and country of import. The animals found positive were imported from US, therefore there is a need to conduct a national epidemiological study on BLV to find out its true picture...
Tanveer Hussain, Masroor Ellahi Babar*, Akhtar Ali and Fiaz Hussain
...was selected for further analysis. Dual peaks/heterozygous conditions were identified at positions c.36 (A/C), c.87 and c.225 (Y, T/C). The phylogenetic tree was constructed through MEGA 6.1 software package using the Neighbor Joining method with a bootstrap value of 1000. Finally, the dromedary camel sequences from Pakistan were compared with corresponding genetic sequences of other animal species available on NCBI including European bison, Indian bison, Tibe...
Junyan Bai*, Zhihao Dong, Youbing Yang, Ziheng Li, Xiaoning Lu and Huirong Gong
...e: small;">A comparative analysis of meat quality traits at different segmented parts of small-tailed Han sheep was conducted. The results showed that drip losses of back and tendon were obviously lower than those at other parts (P<0.05). Cooking loss of tendon was significantly lower than that at other parts (P<0.05). The shearing force of hind leg was obviously lower than that at other parts (P<0.05). The flesh color of waist wa...

Dabesa Wegari and Fikiru Temesgen Gelata*

 

 

...rsonal observation. Data analysis was made using descriptive and inferential statistics. In the study areas, the major beef cattle value chain actors were input suppliers, producers, traders, fatteners, processors, retailers, consumers and governmental and non-governmental institutions. The core functions of the chain actors were input supply, production, marketing/trading, processing, retailing and consumption of the product.Therefore, the required recommenda...

Faiza Aman1,*, Neelam Ara1 and Syed Mehar Ali Shah2

 

 

...o first picking. Cluster analysis for quantitative traits grouped pea genotypes into five different clusters at a dissimilarity level of 0.61 which also depicted the presence of high magnitude of variation among the studied genotypes. On the basis of superior performance for maturity, yield and yield-related traits, pea genotypes Leena Pak, UAP-47, UAP-29 and Green Gold are recommended for testing across locations and for onward use in pea breeding programs.
Hafiz Hammad1, Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Abid Niaz4 and Muhammad Arif4
...l software Statistix 8.1 analysis of variance technique and significant of treatments was tested using LSD test at probability level of 5%. The maximum P accumulation (178.37 mg P pot-1), P uptake efficiency (1.90) and PE (phosphorus efficiency) ratio(747.15 g DM (g plant-1) of sunflower was observed with application of NK + Rock phosphate at 7 g/pot + Wheat straw at 28 g pot-1 (T6) in cultivar Hysun-33. While, the l...
Sheza Shehzadi1, Mohammad Umar Farooq2*, Rukhsana Kausar1, Ijaz Ali1*, Muhammad Arshad Ullah3 and Maqbool Shahbaz4
...along with the proximate analysis including protein content, crude fiber, ether extract, and total digestible nutrients. Significant results were obtained by applying ANOVA test on the data retrieved. All three factors (BP, CC and CS) showed maximum results after 60 days of growth. The result indicated that after the 60 days interval the animal unit per month gave the maximum fodder (7.0667 AU/M/Ha) that proposed to be grazed for large ruminants and then 21 sm...
Kartika Noerwijati1*, Sholihin Sholihin1, Tinuk Sri Wahyuni1, Rohmad Budiono2, Nguyen Van Minh3, Roy Hendroko Setyobudi4, Zane Vincēviča-Gaile5 and Lulu Husna6
 
 
...lones, therefore cluster analysis (the K-means cluster analysis method) was carried out to classify clones based on tuber yield and harvest index and to facilitate the selection process. The aim of this study wasto select high yielding cassava clones as selection material for the next stage of selection in the highlands. The F1 seedlings population selected, had a wide range of tuber yield. The results of this stu...
Grace Okongor, Chukwudi Njoku*, Pauline Essoka and Joel Efiong
...ltiple linear regression analysis. The result revealed that only sunshine intensity was a significant predictor of yam yield (p =0.011), whereas rain days (p = 0.332
Muhammad Tariq Chaudhary1, Saleem Ashraf 2, Muhammad Yaseen3 and Muhammad Luqman3
 
 
...ment process. Regression analysis affirmed that extra-curricular activities (P<0.05), being a sportsman, (P<0.01), higher grades in examination (P<0.05), educational environment at IATI Sargodha (P<0.01) and Rahim Yar Khan (P<0.05) were the prominent determinants of their employability. Study concludes that educational activities and co-curricular activities were equally important for the successful academic performance and earning an employabil...
Sana* and Abbas Ullah Jan
 
...time involved). Data analysis revealed that 34.7 percent of children of the sampled farm households were full-time involved and 41.9 percent were part-time involved in different agricultural activities. The most frequent of them were animals grazing, fodder cutting and carrying, crops weeding, harvesting and firewood collection. Multinomial Logistic analysis revealed that a child’s age, gender (male) and schoo...
Jiawei Hong1,2, Mingqiang Chen 1,2, Zhenghua Deng1,2, Youning Li1,2 and Yu Wang1,2*
...ical clustering heat map analysis and principal component analysis (PCA) showed that the microbial community of the high dosage group (10 mg/L) was clearly different from the other two groups. Overall, enrofloxacin at high dosage of 10 mg/L significantly altered the community diversity of noble scallop. This study characterized the variation regularity of the intestinal microbial of the noble scallop in response to enrofloxa...
Ahmad Abrar Khan* and Muhammad Idrees
...practice. The Regression analysis results reported application of chemical fertilizers, FYM, irrigation in drought periods, pruning, winter oil and Bordeaux mixture application were positively and significantly related to apricot production while simultaneously flat-headed borer and brown rot were found inversely but significantly related. The study recommends special attention to improve stone fruit production through increased contacts with agricultural exte...
Shakeel Ahmed Soomro1,2, Sikandar Almani3, Lal Bux4, Khalil Ahmed Ibupoto2, Muhammad Sohail Memon2, Farman Ali Chandio2, Shakeel Hussain Chattha2, Zaheer Ahmed Khan2, Zubair Ahmed Soomro5,Babar Mustafa Ansari6 and Kunjie Chen1*
...rror and significance of analysis of variance which is essential for testing the applicability of the model. Predicted optimum temperature and time were 63 ºC and 142.5 min respectively and predicted length, width, thickness, TKW, bulk density, true density, porosity and hardness under these conditions were 6.72mm, 3.32mm, 2.27mm, 27.38 g, 716.78 kg m-3, 1459.14 kg m-3, 51.24% and 45.26 N respectively. The results further revealed th...
Md. Sabbir Ahamed1, Md. Rayhan Chowdhury1, Md. Atik Mas-ud1, Sujat Ahmed2, Md. Shahadat Hossain1 and Mohammad Nurul Matin1
 
 

 Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*

Kamran Baksh Soomro1*, Sina Alaghmand2, Sanyogita Andriyas3, Muhammad Rehmatullah Khan4, Naveedullah5 and Amin Talei6
 
 
Roland Eric Yessinou1*, Alain Richi Kamga Waladjo2, Nestor Noudeke1, Ignace Dramou3, Justin Adinsi1, Victorien Tamègnon Dougnon4, Elsie Yélognisê Sangnidjo5, Razack Osse5, Alfred Dansou6 and Souaïbou Farougou1
...by host and country, and analysis conducted to determine pooled prevalence estimates for each category Analysis was done using the metaprop command in STATA version 15 and MetaXL software. A total of 18.111 individual samples from 11 countries, described in 34 articles were studied. Analysis of twenty-six studies indicated that the pooled prevalence was 19.0% [95% CI (15.0-23.0 %), I2&thin...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
...wild birds. Phylogenetic analysis of recent Egyptian isolates in Menofia governorate, Egypt was constructed, and showed to be of genotype VII. Eight NDV viruses were isolated from vaccinated commercial broiler flocks showing respiratory manifestation in Menofia governorate, Egypt during 2019. Those viruses showing deaths and haemorages of inoculated SPF ECE eggs, harvested allantoic fluids showed haemagglutination activity by using 1% RBCs and also haemaggluti...

 Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2

...ustify;">The biochemical analysis and the determination of the intracellular-extracellular contents of the sperm cells are important in the spermatological studies. Since homogenization processes reveal the intracellular contents of the sperm cells, we aimed to examine the optimal sonication procedure and the protective effect of the antioxidants against sonication. Four merino rams (2–3 years old) were used for semen collection, and the ejaculates were ...
A.E.S. Patrick1,*, S. Kuganathan2 and U. Edirisinghe3
...mall;">Linear regression analysis of length-weight relationships (LWRs) were studied to reveal the growth patterns of 380 specimens belonging to 10 species, 9 genera and 5 families of local fish in Vavuniya reservoir, Sri Lanka during January, 2013 to August, 2014. The regression coefficient value ‘b’ from LWR and statistical comparison from the ideal value (b=3) were used to find the growth patterns. Most of the local species showed ...

Joseph Anejo-Okopi1*, Jennifer Ifeoma Okpara1, James Mogoret Dabol1, Joshua Adetunji1, David Adeniyi2, David O. Amanyi3, Otobo I. Ujah4, Veronica David1, Patience Omaiye5 and Onyemocho Audu6

...8.9%. In the statistical analysis, the HBV-mono infected; age (p=.037), marital status (p=.001), cigarette smoking (p=.022) and condom use (p=.04) were significantly related to HDV infection. The study demonstrated high prevalence of HDV infection, which is imperative for policy makers and health care providers to strengthen the prevention measures for HDV, including the use of HBV prevention vaccine. Also, it is important to screen chronic HBV patients for HD...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

...followed by phylogenetic analysis. Positive samples were isolated on Vero cell culture and identified by TEM and immune-peroxidase technique. Betacoronavirus 1 was detected by ELISA in 6 out of 20 fecal samples (30%), PCR detected 4 out of 6 ELISA positive samples at specific M.W. band of 236 bp by electrophoresis, and one sample was sequenced and submitted on Genbank with acc.no. MW173144, further phylogenetic sequence analysis...
Bram Brahmantiyo1,*, Henny Nuraini2,3, Astari Wibiayu Putri3, Maizirwan Mel4,5 and Cecep Hidayat6 
 

...ign with unbalanced data analysis was used for quantitative traits and descriptive analysis for qualitative traits. Qualitative characteristics of hair color variation of Hyla rabbit was different than other with 7.7 % pointed on their white hair color, and hip type compact of Hycole, Hyla and New Zealand White were 90 %, 84.6 % and 76.9 %, respectively. Quantitative characteristics of chest depth of Hycole and Hyla rabbits ...
Titin Herawati1,*, Indra Adiwiguna1, Ayi Yustiati1, Iis Rostini1, Roy Hendroko Setyobudi2, Hafizan Juahir3 and Asep Sahidin1
 

... and water quality. Data analysis employed comparative-descriptive method by mapping the species and comparing the diversity of fish living in both ends of Jatigede Dam. The results indicate that six families of fish are identified, consisting of 14 species. The fish diversity of Cimanuk River upstream Jatigede Reservoir is of moderate one – 1.735≤H’≤1.909 – with low species dominance, while containing high equitability 0.61≤E≤0.7...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
 

Faheem Aslam1,*, Aneel Salman2, Inayatullah Jan3 and Sarah Siddiq Aneel4

...12) through quantitative analysis of textual data. The text mining of these water policies enables to analyze the massive amounts of information quickly, making connections and surface important links between entities. The results show that Pakistan’s National Water Policy, 2017 is more comprehensive as compared to India’s National Water Policy, 2012. India’s water policy distinctively prioritizes industrial growth at the cost of its agricult...
Jun Yan Bai*, Zhi Hao Dong, Yu Chen, Jing Yun Li, Ying Lei, You Bing Yang, Kun Peng Shi and Xue Yan Fu
Saima Siddiqui1, Ghulam Hussain Abro1, Tajwar Sultana Syed 1Abdul Sattar Buriro2, Sohail Ahmad3, Muhammad Zeeshan Majeed4 and Muhammad Asam Riaz4*

 

...ion. Stepwise regression analysis revealed that TDM, TDL and GGL (Gossypol gland on lamina) contributed 27.5%, 33.9% and 36.6% in population variation of jassid, whitefly and thrips, respectively. High TDM and TDV was considered morphological marker for jassid while low TDL, TDM and TDV was thought of as morphological marker for whitefly. The cotton variety 3-508 OP having relatively less dense but long TLL (Trichome length on lamina), TLM (Trichome length on ...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Ying Wang1, Ding Zhang1, Yu-hai Bi1,2, Ali Raza Jahejo1,Qian-qian Cheng1 and Wen-xia Tian1,*
...qRT-PCR and Western blot analysis were performed to identify the expression levels of PG-related genes (COX-2, GSTA3, PTGDS, PTGES, PTGER3 and PTGER4) in erythrocytes of H9N2 infected chickens at time intervals of 0, 2, 6 and 10 h and P < 0.05 was considered as statistically significant. Expression levels were increased significantly in case of COX-2 at 0 and 6 h, GSTA3 and PTGDS at 0 h and PTGER4 at 2 h of infection, while decreased expression was o...
Rukhsar Ali* and Ali Muhammad
 

...terval. Based on sensory analysis, the best suitable blend was selected within different ratios of mango pulp and loquat pulp. The blended pulp with ratio (60:40) got the highest mean score of judges for overall acceptability which used for further study. The different treatments were included in this study as MLBPr0 was control with no preservatives, MLBPr1 having 0.1% PS, MLBPr2 having 0.1% KMS, MLBPr3 having 0.1% ...
Muhammad Farooq1*, Allah Rakha2,Jawad Ul Hassan2, Iftikhar Ahmed Solangi1, A. Shakoor3, Muhammad Bakhtiar4, Muhammad Noman Khan5,Shoaib Khan5, Ibrar Ahmad6, Shabir Ahmed7 andWang Yunyang1*
 

...f muffins. The proximate analysis of mushroom based powder and wheat flour was also performed. After this loaf weight and volume, structural examination, sensual attributes of muffin were also performed. The effect of mushroom powder on moisture, crude protein crude fat and nitrogen free extract of muffin were non-significant and effect on ash and crude fiber were significant when 0%, 10%, 20%, 30% and 40% mushroom powder based muffin were prepared. The effect...

Mustasim Famous1*, Asim Chakrabartty Aditya2, Shahabuddin Ahmed3 and Sabuj Sutradhar2

...Judging from the overall analysis of the results, it may be concluded that production and reproduction performances of Holstein crossbred are superior to other dairy crossbred in Kishoreganj, Bangladesh.

...

Naveed Wahid Awan1, Asif Ali Abro2* and Ahmed Raza ul Mustafa3

...ough rank and regression analysis. The rank analysis was done based on crop production, the area cultivated, yield, use of fertilizer, number of tube-wells and their respective prices. It was observed that Punjab province holds tops position with respect to the factors analyzed in the study. In regression analysis, the climatic index was found negative and significant, whereas agricultural...

Raza Ullah Khan1*, Ahmad Khan1, Mohammad Zameer Khan1, Fayyaz Hussain1, Zafar Islam2 and Muhammad Asad Hameed2

...n spectrophotometer. The analysis showed the presence of hydrophobic and hydrophilic sites. The Fourier infrared spectroscopy analysis showed spectrum bends at different wavenumbers while elemental analysis show existence of different macro and micro nutrients which can be used to supplement the plant needs for nutrients by incorporation of humic substances along with commercial inorganic ...
Tai-hua Jin1, An-gang Lou2,Jiu-xiu Ji1, Cheng-dou Cui2, Long-zheng Yu2 andLi-zeng Guan1*
...Targetscan and RNAhybrid analysis software and Dual-Luciferase reporter gene system was used to analyze the regulation mechanism between miR-1468 and GH. The results showed that the GH mRNA and protein level in pituitary cells of Yanbian yellow cattle could be significantly decreased by adding miR-1468 mimics (P<0.01), while these were significantly increased by adding miR-1468 inhibitor (P<0.05). The results of bioinformatics...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...ifferences. Phylogenetic analysis and evolutionary divergence were also estimated. The sequence was submitted to GenBank (NCBI) with accession number MN712202. Our results represent a preliminary step towards the identification of polymorphisms in STAT5A gene of Nili Ravi Buffalo. Further studies are required for the association of genetic polymorphisms of STAT5A gene with fertility related traits.
...
Jing Yu1*, Jiangmei Mao1,2 and Dongliang Wang1,3
...er adopted the inversion analysis of the primary productivity (PP) in the Daya Bay, China applying the vertical generalized production model (VGPM) with ocean color and sea surface temperature (SST) provided by MODIS-Aqua. On the basis of modified VGPM model, fishery resources in the Daya Bay were evaluated according to the Tait model, Cushing model, and trophic dynamics model. Results showed that the seasonal variation of PP was obvious in the bay, the averag...
Ahmed Saud Alsaqufi1, Sheikh Mustafizur Rahman2,3*, Roshmon Thomas Mathew2, Yousef Ahmed Alkhamis1,2, Md. Moshiur Rahman3,4 and Muhammed Aslam Pathiri2
...ed for phenotypic traits analysis. The results revealed that complete darkness (24D) significantly improved the overall growth parameters (total length, standard length, caudal peduncle length, anal fin length, caudal fin length, total body depth, dorsal fin length, pre dorsal distance, pre anal distance, pre ventral distance, head width, head length, eye diameter, maxillary barbell length, inter orbital length, and specific growth rate) than all other treatme...
Hesham Saeed1*, Manal Abdel-Fattah1, Ahmad Eldoksh1, Farid S. Ataya2 and Manal Shalaby3
...nscription-PCR. Sequence analysis of that gene showed a 564-bp encoding a protein of 187 amino acids with a predicted molecular weight of 21 kDa. Basic local alignment search tool (BLAST) sequence analysis revealed that C. dromedarius IFNα gene shares high sequence identity with IFNα genes of other species, including C. ferus, Vicugna pacos, and Homo sapiens. Expression of C. dromed...
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
...puncture for biochemical analysis. Morphometric observations revealed significant decrease (P≤0.001) in body weight of mice in Al treated groups as compared to control but organs weight increased contrary to the body weight. Significant increase (P≤0.01-P≤0.001) was recorded in total alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) in liver and urea, and creatinine levels in kidneys, in Al exposed groups against con...
J.Y. Bai1,*, R.T.D. Wu2,*, Q. Zhang2, D. Bao2, L.M. Dao2 and X.H. Tian3
Rida Haroon Durrani1*, Fareeha Akhtar1, Ali Ahmad Sheikh1, Munazzah Maqbool1, Aleena Kokab1, Kiran Imtiaz2 and Muhammad Ilyas Riaz3
...tal systems. Statistical analysis revealed that temperature and seasonal variations alongside storage conditions affect not only the shelf-life of fish meat but also increase the spoilage microbiota population, hence, altering the quality profile of meat. Therefore, we can conclude that storage temperature is a crucial factor in determining the quality of fish meat.
...
Aiguo Zhou1,2, Di Sun1,2,Shulin Liu 1,2, Yongyong Feng1,2, Yue Zhang3
Yanfeng Chen4, Shaolin Xie1,2* and Jixing Zou1,2*

 

...hannidae family. And the analysis of sequences showed that there were nine haplotypes in 60 individuals, which were eight unique and one shared haplotypes. In addition, the genetic distance for inter-species of all the haplotypes ranged from 0.000 to 0.004. And the mean pair-wise genetic distance between the two color morphs were estimated to be 0.001. This indicated that the “Bicolor” and “White” types of northern snakehead belong to t...

Bushra Baig and Saeeda Yousaf*

...er and Neem.Quantitative analysis indicated 21 active componentsin Black pepper which were dominated byCaryophyllene (32.3%), Delta-3-carene (18.7%) and Cinene(15.5%). Neem showed 17 active components, among them Beta-Pinene (14.8%), Delta-Elemene (14.4%) and Palmitic acid (13.2%) were dominant. The mix-biopesticide of Black pepper and Neem in comparison to their individual impact showed the highest insecticidal efficacy against whitefly and aphids. The plots ...

Jabeen Farheen1,2*, Simeen Mansoor1 and Maria Abid1

...creased. The macroscopic analysis manifested that allura red had 95% broken-ended extremely thin and transparent root tips at 1% concentration. In the microscopic analysis, FCAs induced eight different kinds of chromosomal aberrations in which allura red significantly induced the highest frequencies of abnormal prophase, sticky metaphase, c-mitosis, sticky anaphase, and laggard anaphase at each concentration. While, dislocat...
Mohammed  Akinlabi Rufai1*, Abideen Akinkunmi Wahab2, Kamil Ayo Fasasi1, Quadri Olusegun Adeshina1 and Mary Tolulope Awotidebe1
...application. Statistical analysis conducted using analysis of variance indicated that there was no significant difference in the overall biocidal activities of H. bacteriophora at 5% confidence limit (F>0.05). Statistical test conducted on the data also supports the reliability of the data with a correlation coefficient of 0.85<R2<1.

...
Imran Tarique1*, Muhammad Ghiasuddin Shah1, Illahi Bux Kalhoro1
Zaheer Ahmed Nizamani2, Mansoor Tariq 2, Jamil Ahmed Gandahi1
Saqib Ali Fazlani3 and Benazir Sahito3
...tology and micrometrical analysis in skin of different body regions (dewlap, abdomen, scrotum / udder and thigh) of young and adult of either sex of red Sindhi cattle (Bos indicus). Skin of red Sindhi cattle showed epidermis thickness rang of 41.1-50.13 µm and dermis thickness rang of 443.97-597.05 µm in all body regions and significant (0.05) in age and sex group. The epidermis possessed strat...

Hafiz Basheer Ahmad1*, Sajida Habib2, Waqas Shafqat Chattha3, Rizwana Qamar2, Shazia Saeed2, Abdul Khaliq1 and Salsabeel Rauf2

...ed by using multivariate analysis. The results revealed that the mean values of all traits except for days to 50% flowering of maintainer lines were higher than restorer lines. The principal component analysis for B-lines showed that first eigen value equals 2.77 and represented 39.57 % of the total variability while second eigenvalue equals 1.24 and showed 17.76% of the total variability. The first two factors showed 57.34%...

Syed Ismat Hussain1, Muhammad Ramzan Asi1, Habbib Anwar1, Faqeer Ahmad1, Ateeq ur Rehman2*, Muhammad Shahid3, Syed Atif Hasan Naqvi2*, Ummad ud Din Umar2, Muhammad Asif Zulfiqar4, Memuna Ijaz1 and Hafiz Usman Shakir1

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sher Muhammad3, Muhammad Latif4, Ayesha Zafar1, Imran Shehzad1, Sarfraz Hussain5, Aneela Riaz6 and Abid Niaz2

...rom all pots. Laboratory analysis for collected plant samples was carried out. Data were statistically analyzed. Results indicated that treatment (T8) produce maximum nitrogen (3.27%), phosphorus (1.0%) and potassium (2.85%) concentration in shoots of maize plants. The same trend of improvement was noted for maximum nitrogen (3.16%), phosphorus (0.92%) and potassium (2.88%) concentration in roots of maize plants.

...

Taqi Raza1*, Kashif Nazir Qureshi2, Shakeel Imran2, Neal Samuel Eash3 and Imma Bortone4

... and Fe) and other basic analysis (pH, EC, TSS and TDS). Effluent samples were collected from the discharge of industries entering into main wastewater collecting channel located in Faisalabad city. Heavy metals contents were determined via atomic absorption spectrophotometry. Results showed that the highest pH (11.06) was recorded in textile effluent while EC (7.89mS/cm), TSS (1185.55mg/L) and TDS (6317.33mg/L) were found highest in chemical industrial efflue...

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sarfraz Hussain3, Ayesha Zafar1, Imran Shehzad1 and Aneela Riaz4

...maize plants. Laboratory analysis for collected soil samples was carried out. Data were statistically analyzed. Results indicated that treatment (T8) produce maximum plant height (115.0 cm), biomass (64.34 g) and root length (27.083 cm) of maize. Organic matter content (1.78 %), phosphorus (16.67 ppm) and potassium (213.0 ppm) concentration in soil was also increased in this treatment.

...
Suman Bhattarai1*, Subodh Raj Pandey2, Jaya Prakash Dutta3, Meghnath Timalsena4 and Rajendra Bam5
...t-test were used for the analysis of collected data. Multiple regression model revealed that family labor (p=0.68), age(p=0.007), family size (p=0.001), experience (p=0.007), harvesting (p=0.000) and maintenance of flowering plants (p=0.057) have significant effect on honeybee productivity. Labour cost and migration cost had positive coefficient and significant relation at 1% level of significance with gross return whereas expenses on baiting materials had pos...

Alim-un-Nisa1*, Sajila Hina1, Imran Kalim1, Mohammad Khalid Saeed1, Ijaz Ahmad1, Naseem Zahra1, Sania Mazhar1, Shahid Masood1, Muhammad Ashraf1, Qurat-ul-Ain Syed1 and Rabia Shad2

...5°C. Microbiological analysis of candies and beet powder at 4°C and 25°C ensure that they are safe for consumption at both storage temperatures. 

...

Chala Hailu Hussen and Fikiru Temesgen Geleta

...n model was employed for analysis. The descriptive statistics revealed that 127 sampled farmers participated in cluster crop production, while the remaining 33 were not. On the other hand, the result of probit model regression had shown that household-related factors such as being a male, economic dependency ratio, farmer experience, marketable surplus supply, and seed multiplication objectives were positively affecting the probability of participation decisio...
Amreen Zahra1, Mushtaq A. Saleem1*, Hasnain Javed2, Muhammad Azmat Ullah Khan3, Muhammad Naveed1 and Abdul Rauf Shakoori4
...g approaches. Mutational analysis in our study revealed S61A, S61M, S61Y, S61G, S61Q and M90L as the most hypervirulent mutations. This induces a selection pressure and a rate of increased virulency on Gag-Pol cleavage sites. These results significantly highlight the fact that the identified SNPs possibly contribute towards a positive selection pressure contributing to the identification of novel mutations like S61A, S61M and M90L at Gag-Pol cleavage sites har...

Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*

... 4% formaldehyde for the analysis of phytoplankton. Subsequently, the phytoplankton were counted under microscope. Result showed that water quality parameters of all fish farms were suitable for farming purpose excluding Shamimul Hassan Farm, Fish Farm Green Co. and Ghulam Ali Nizamani Farm, where high level of total hardness was recorded as 1000±2.65 mg l-1, 700±4.80 mg l-1, and 975±4.73 mg l-1, respectively. The minimum chlorophyll-a (1....

Mian Muhammad Arif* and Malik Muhammad Shafi

...ics was used for general analysis, while budgetary technique was used for profitability analysis. Ordinary Leased Square (OLS) technique was employed to find the effect of different inputs on profit of broiler poultry farms. The ANOVA tool was used to know the variations in cost, revenue and profit of various size of commercial broiler poultry farms. Due to wide spread variations in cost and return pattern of various broiler...

Rabia Iqbal and Muhammad Naeem *

...al for growth parameters analysis. Different growth parameters, such as mean weight gain, mean length gain, specific growth rate, protein efficiency ratio, feed conversion ratio and production were determined. Results revealed maximum gains in T3 (25%) feed group as compared to T1 (15%) and T2 (20%) feed groups. The lowest food conversion ratio (FCR) was also noted in the T3 (25%) group showing best feed composition of T3 (25%) feed by the tested fish fry. ANO...

Summara Abbasi* and Khalid Nawab

...ted 333 respondents. For analysis of data descriptive statistics and binary logistic model was used through the Statistical Package for Social Sciences (SPSS) and STATA. Information about dairy practices was studied and percentages recorded, about 70% of the farmers were aware of artificial insemination and vaccination while at least 51% was reported about livestock management. Improved feeding was very active as 70% of farmers were well informed followed by b...

Mehnaz Safdar1, Urooba Pervaiz1* and Dawood Jan2

...g technique. Descriptive analysis revealed that 305 women farmers were involved in rearing poultry. A 5 point Likert scale was used, showed that women farmers had various constraints out of which Pardah, mobility and lack of information source were ranked on top with Likert score value of 4.12, 4.00 and 3.94, respectively. Due to male dominant society, 62.8% of the decisions were taken by spouse. Results of Chi-square analysis
Maria Qibtia1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Sehrish Faryal1, Ahmed Mansouri2, Zeeshan Ahmad2, Muhammad Hamid3 and Ali Raza Awan1,*
...me, focuses on molecular analysis of LCT-gene from Pakistani patients with LNP.
...

Muhammad Qaddafi Khan Wali, Arif Alam* and Ikram Shah

...e used binary regression analysis to identify factors affecting food security in the target area. This investigation discloses that 90 % of the sampled households are suffering from food insecurity. From the exponential values of coefficient binary logistic regression, it is concluded that Gender, Occupation, Landholding, Income, Low income, High population, remoteness, Low landholding, and firing online of control affected food security in the mountain areas....

Muhammad Tariq Mahmood1*, Muhmmad Akhtar2, Kaiser Latif Cheema2, Mushtaq Ahmad1, Muhammad Jahanzaib Khalid2, Afzal Zahid2, Muhammad Kashif3 and Zeshan Ali4

...lot and mega environment analysis were employed to identify the most stable and high yielding chickpea advance strains. GGE biplot among PC1 and PC2 illustrated that G-10 (K-15019) was ideal genotype performing best across the environments. Higher yield potential coupled with more yield stability across all test environments was recorded in G-10 (K-15019) followed by G-2 (CH47/12), G-12 (K-15001), G-15(CH66/10) and G-16(CH53/12). Mega environment

Ayaz Ahmed* and Muhammad Zulfiqar

...c regression. Odds ratio analysis of the social and natural assets data showed that the Joint Forest Management approach had significantly increased the social and natural assets of the participants. In terms of financial asset, odds ratio in favor of ‘NTFPs income’ and ‘employment opportunities’ for JFM sample households were 2.289, 2.501 times respectively more than non-JFM sample households. Trainings imparted by forest department si...

Arshad Khan1, Mohammad Ihsan1, Mohammad Nisar1, Ali Hazrat1*, Murad Ali3, Rashid Ul-Haq3, Khalid Khan2, Karishma Gul1 and Shah Faisal1 

...tal seed storage protein analysis was 17.5%. In Band, the 14 total genetic diversity was (0.60%) followed by Band 16 (0.58%) and Band 17 (0.55%). Similarly, Band 3 showed diversity, while Band 8, 4 and 5 indicated 0.45, 0.38 and 0.35%, respectively. A cluster dendrogram tree was constructed which was divided into two linkage and further divided into seven clusters. The variation was found among the clusters. The information obtained from the study is useful in...

Muhammad Sarfraz1*, Muhammad Amjad Qureshi2, Muhammad Arshad Ullah3, Shahzada Munawar Mehdi4, Abdul Rasool Naseem1, Sarfraz Hussain1, Muhammad Qaisar Nawaz1 and Muhammad Rizwan1

...d irrigation water. Soil analysis at harvest showed that soil was improved with canal water with GR of soil trailed by saline water with GR of soil and irrigation water. The least variations in soil pH, EC and SAR were observed in control where nothing was applied. Comprehensive studies in different ecological zones with salt affected soils with variety of saline water may be grown to confer these approaches. 

...

Abdul Hameed1*, Ihtsham Ul Haq Padda1 and Abdul Salam2

...Micronutrient deficiency analysis shows that 22% of the survey households in Punjab, 30% in Sindh, 11% in KP, and 37% in Balochistan are suffering from iron deficiency. Besides, 57% of households in Balochistan, 56% in Sindh, 35% in Punjab, and 26% in KP experience deficiency of zinc. The vulnerability analysis of the survey data found 12% of the households at the national level to be extremely vulnerable and food insecure i...
Muhammad Mohsin1, Yin Hengbin2*, Qian Chong3 and Bohyun Kim4
...., catch and effort data analysis (CEDA) and a stock production model incorporating covariates (ASPIC). In CEDA, FM showed highest value of R2 (0.897) and computed maximum sustainable yield (MSY) as 2471 t. On the other hand, in ASPIC, FM also produced highest R2 value (0.931) and estimated MSY as 2867 t. Obtained results indicate that fishing mortality (F) and biomass (B) showed increasing and declining trend from 2000 to 2009, in that o...
Ghulam Raza1*, Maqsood Anawar2, Muhammad Akbar1, Muhammad Ali1, Alamdar Hussain1, Azhar Hussain3 and Tanveer Hussain4
...e characteristic habitat analysis and preference was important as the ibex move downwards during winter to cope the fodder shortage after heavy snowfall. This may expose the ibex to potential threats of predation by predators, poaching and competition for food with livestock during winter season. Therefore, the study was conducted to analyze the characteristic habitat feature and preference of Himalayan ibex during winter season in Hushe valley of Karakorum ra...
Nilgun Dogan1,* and Hakan Adanacioglu2
...itability and efficiency analysis. The cost of a unit of honey was US$3.16 and the average price paid to the beekeepers was US$6.78. The absolute and relative profits were calculated as US$3.62 and US$2.14, respectively. The efficiency of the beekeeping farms was measured using Data Envelopment Analysis. The results of output- and input-oriented constant return-to-scale (CRS) analysis indi...
Zeynep Sonmez1 and Memis Ozdemir2,*
...strate 8). After the PCR analysis of the identified gene regions of 72 head Holstein cattle, samples were separated by the high-resolution melting analysis (HRMA), and sequence analysis was applied to some randomly selected samples. New polymorphisms were identified in the determined genes and gene regions as a result of the sequence analysis. In the 1
Rahma Mohamed, Sara Nader, Dalia Hamza and Maha A. Sabry*
...athogen. Bacteriological analysis of nasal swabs of 52 diseased and healthy donkeys at different localities in Egypt revealed that the overall occurrence of Cryptococcus spp. was 11.5%. The highest proportion was in El-Fayoum Governorate (25). Phenotypic identification of Cryptococcus indicated that 13.2 % and 7.1% among healthy and diseased donkeys were positive for this pathogen, respectively.The study of the potential risk factors associated w...

Huma Qamar1, Mariam Hassan1, Muhammad Zubair1*, Adnan Arshad2, Muhammad Qusain Saeed3, Muhammad Umar3, Sundas Shahzad1, Tariq Mahmood1 and Muhammad Aftab1

... their path co-efficient analysis. Mean square for five traits was constructed which was highly significant except plant height. Correlation in plant’s height and branches, days required for flowering and days required for maturity was positive at genotypic as well as phenotypic levels. Correlation between numbers of branches and days required for flowering was positive, while negative association of branches’ number was observed with days required...

Ahmed A. Kheder

... sequences. Phylogenetic analysis results indicated that SLRSV-Eg isolate under study (acc. no. MT648777.1), showed 65.9 – 99.5% nucleotide similarity with available homologous sequences from other crops. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay which is one of the most promising molecular diagnostic techniques was applied. The amplified products were analyzed using amplification curves and electrophoresis. In this stu...

Iftikhar Jan* and Sahib Alam

...akistan. The mycological analysis of the samples demonstrated that the highest population of Aspergillus section Flavi was existed in soil (n = 236) than in maize grain samples (n = 198). Across all the four zones, the highest population of Aspergillus section Flavi was observed in AEZ-C (n = 115) whereas the lowest population was found in AEZ-B (n= 99). The isolates of Aspergillus section Flavi were then characterized for their aflatoxigenicity and nonaflatox...

Shahzad Khan1*, Munir Khan1, Arif Alam2, Ikram Shah2, Mahfooz Khan1 and Fida Muhammad Khan1

...cted to perform economic analysis and to elaborate the determinants of wheat crop in district Charsadda, Khyber Pakhtunkhwa, Pakistan in the year 2018. Three villages, namely, Aspandehri, Kamran Kalay and Sarfaraz Kalay, were purposively selected. A sample size of 41 wheat growers from farm households was chosen from these villages for data collection. Thus, selection of the farmers was based on the proportional allocation technique. However, primary data were...

 Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*

...0-70 nm in diameter. PCR analysis of
SGH homogenate with virus specific primer pair resulted in DNA
fragment with expected size as previously mention in literature. These
data demonstrate the presence of the salivary gland hypertrophy virus in
house fly population in Egypt which provides the opportunity to evaluate
the potential use of this virus to control the house fly population in Egypt

Muhammad Akhtar1, Muhammad Tariq Mahmood2*, Kaiser Latif Cheema1, Mushtaq Ahmad2, Muhammad Jahanzaib Khalid1, Amir Amin1, Javed Anwar Shah1, Zeeshan Qadeer1 and Zeshan Ali3

...ckpea lines. Correlation analysis showed that the strains possessing higher number of pods plant-1, more root length and maximum grain weight were comparatively least susceptible to moisture stress along with higher yield potential. Results regarding drought indices also indicated that relatively higher DTE, less DSI and minimum yield reduction percentage were revealed by KK-10001, KK-10015, KK-10019 and Bhakkar-2011. Therefore, these advance lines possess bes...

Ain-ul-Abad Syed1*, Zaheer Ahmed Khan1, Shakeel Hussain Chattha1, Irfan Ahmed Shaikh2, Mian Noor Hussain Asghar Ali1, Zohaib ur Rehman Bughio3, Shahzad Hussain Dahri2 and Ghous Bakhsh Buriro4

...It is inferred from this analysis that hydroponic cultivation is successful in saving water and fertilizer and increasing crop yields at the desirable limit. To enhance yield, the hydroponic model should be equipped with sensors and artificial intelligence technologies to handle difficulties related with food supplies. The hydroponic growing technology should be introduced to cities. At the time, such as when challenged with Covid-19 and the locust attack.

...

NawalM. Abdulla1, M. Haroun1*,Mohamed A. Shalaby2and Ahmed A. Elsanousi2

...v>fluids (AAF). Sequence analysis and phylogensis of the NDVQF13 strain using 150bp
primer sets revealed 112R-R-Q-K-R-F117 amino acid motif confirming velogenicity of the
strain and its relation to genotype group VII, respectively. NDVQF13 failed qualification
for sequence and phylogentic analysis using the same primers.
...
Ala’audin Hakami,1AusamaA Yousif,2* Mohamed Zaki,3 Mahmoud Ismail,4,5 Ahmed Al-Ali,5 Abdu-Rahman Al-Ankari5 
...otide-based phylogenetic analysis clustered one
African grey parrot PBFDV (Mira08) with South-East Asian and
Australian isolates indicating interspecies transmission.
...

Mokbel, Samah A.1,Ahmed K. El Attar1; Azza G.Farag2.

...uencing and phylogenetic analysis of the 16S
rRNA gene fragment from the hibiscus witches' broom phytoplasma showed 100%
homology with the same region of thehibiscus witches' broom strain thatisolatedin Brazil
(PhytoplasmaBrasiliense). The DNA sequence was submitted for the gene bank as the
first report of the Brasiliensephytoplasma associated witches‟ broom affecting hibiscus in
Egypt.
...

Maha I. El Zaafarany1, WafaaM. Badawy2, Eman M. El Salakh3

...s 28 % (15/54). Sequence analysis in a subgroup of cases indicates the prevalence of high risk HPV16. In conclusion, the present study demonstrated for the presence of HPV in Egypt in a proportion of the malignant breast tissues. This finding suggests that HPV may have a biological significance in breast carcinogenesis.

...

Amal Mahmoud1&2 and Medhat H. Hashem3

...ag1 and 7). Phylogenetic analysis of the nucleotide sequence and the amino acid of the pol or env genes revealed that 7 out of the 8 isolates were closely related to genotype D. Only Sohag 1-HCC isolate was clustered with genotype C which is known as risk factor of HCC. Multiple sequence alignment resulted in unique amino acid substitutions (V320→L and N464→T) and (I284→L, T300→I and K334→R) in pol and env genes respectively. Further p...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
... technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sharkia/2013/ FMD / (KJ210080) revealed (99–100%) identity with Egypt 2012/ and Palestinian-Gaza virus. Phylogenetic analysis of serotype O of this study from Ismailia (KJ210076) , Alexandria (KJ210074) and El-Mania (KJ210077) showed 100% identity with each other and 92% identities with Asian type O sequences from Pakistan, Iran and Turkey while FMD/A/3D/Egypt/...

Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3

...in samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conducted. The % of nucleotide identities of the Egyptian recent isolate (Egy/cairo /2014) with three previous Egyptian isolates were 98.4-98 and 98.4% respectively. Egy/cairo/14 share nucleotide identity with the vaccinal strain flury LEP and kelev with a percentage of (93.1% and 98% respectively). All Egyptian isolates have a commo...

Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3

...S1 subunit. Phylogenetic analysis revealed that seven strains were belonging to variant 2 group of IBV of great similarity with Israeli IB strains, two strains were belonging to IS/1494/06 group like and one strain was belonging to 4/91 group like. This study reports the co-circulation of different IBV serotypes in broiler sectors. Regular monitoring for evolution, epidemiology and genotyping of Egyptian IBV field strains is very important for screening the em...
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H.
M. Mazyad1
...enBank. The phylogenetic analysis of the complete
nucleotide sequence revealed that SLCV is related to other isolates of SLCV from other
governorates in Egypt (Cairo and Ismailia) as well as isolates from Lebanon (SLCV-LB2),
Palestine (SLCV-Pal), Jordan (SLCV-JO) and Israel (SLCV-IL) sharing high identities
ranging from (90% to 97%).
...

Samah A. Mokbel1, Ashraf A. Abd El-Mohsen2

...enhouse. The statistical analysis showed that cultivars and ratio of bed components and their interaction had insignificant (P>0.05) effects on minituber number and minituber weight. However, ratio of bed components and interaction with cultivars were significantly (P<0.05) affected on minituber diameter. Maximum minituber diameter (13.64 mm) was recorded for Lady Rosette cultivar treated with Vermiculite + Sand (4:1), followed by Spunta cultivar (13.53 ...

Ahmed K. El-Attar; Samah A. Mokbel; Ali H. Hamed

...ignment and phylogenetic analysis showed that the
Egyptian isolate of the OYDV is related to other isolates from Japan, Netherlands, Israel,
Brazil, China, India, and Poland with a similarity ranged from 70 to 93% among those
isolates available on GenBank. The virus was eliminated from infected onion plants using
cytokinin (kinetin) in a tissue culture line to obtain the OYDV-free plantlets. The effects
Amro A. Farrag1; Ahmed K. El-Attar1; Om-Hashem M. El-Banna2; Ibrahim A. I.2; H. M. Mazyad 1 
...phylogenetic
analysis showed a relatively high diversity among the three different isolates that the
identity ranged from 89 to 94%.
...
Aly M. Abdel-Salam1, Rehab A. Dawoud2, Amira M.E.Aly2, and Salama M. El-Saghir2
...ies. In addition, IC-PCR analysis on vitroplants
collected from tissue cultures (TC) showed the significant role of TC in spreading out
of BSV. PCR amplicons for the reverse transcriptase and RNaseH motifs of ORF III
for four BSV isolates were cloned, sequenced and submitted to GenBank. The four
accessions showed nucleotide-pairwise identity between 76-82% and amino acid
similarity between 29-63% indi...

Aly M. Abdel-Salam

... NCBI-blastn
analysis for the two isolates with four SCBV accessions retrieved from the GenBank
indicated identity range of 66-91%. Phylogenetic analysis based on amino acid
sequence identity indicated the clustering of the two SCBV in a separate branch from
the other known SCBV; indicating the special nature of the Egyptian SCBV isolates.
Close serologic relationsh...

Amira A. Mazyad1; A. A. Kheder1; Ahmed K. El-Attar1; W. Amer.2; Mahasen, H. Ismail.2; Amal, A.F.1

...uencing and phylogenetic analysis for the coat protein gene. Further insurance of SLRSV infection was performed using light microscopy which showed presence of amorphous inclusion bodies, electron microscopy and chemical analysis.

...
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1 
...sequencing, phylogenetic analysis and the
multiple alignments for the sequences of the Egyptian clones with each other and with the
other sequences of phytoplasma strains on GenBank showed that we may have two
different phytoplasma isolates infecting tomato plants in Egypt.
...

AdlyAbd-Alla1,2, MahaNada3, Francois Cousserans1, and Max Bergoin1

...>ssRNA linear genome and analysis of capsids showed two major proteins of 31 and 32 kDa
and a minor 78 kDa protein. Infection of third instar nymphs by feeding on contaminated food
induced similar disease symptoms and bioassay experiment confirmed virus concentration
response mortality. Injection of a purified virus suspension to third instar larvae of Galleria
melonella and Spodopteralittoralis failed to induce ...

Sadiq Haladu1*, Taiwo Oladoye Akande2 and Mudassir Nasir3

...dl). The result of serum analysis also showed significant difference in all the tested parameters. It was concluded that supplementation of layer diets with 2% fluted pumpkin and moringa improved the health status of the birds and also reduced the egg yolk cholesterol.

...

Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3

...) by (SNT) as well as by analysis of negative
Non Structural Protein sera by solid phase competitive ELISA for differentiation between natural
infected and vaccinated animals by detection of Non-Structral protein of FMDV by priocheck
test. Five hundred sera were collected from vaccinated cattle and buffalo from five Egyptian
governorates (Two governorates represent Delta region: El-Gharbia – Kafr El Sheikh ...

Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*

...ne strains. Phylogenetic analysis for the amplified P4p product revealed close similarity of the FPV isolate (FPLH) and fowl pox vaccine (FPVV) with the published sequences for FPV in the Genebank. Unexpectedly, PPV isolates (PPLA, PPLR) and PPV vaccine (PPVV) showed high similarity to the published FPVs-P4b sequence in gene bank and distributed under genogroup A1 in the phylogenetic tree, while the PPV isolate (PPLH) was classified under genogrop A2 with high...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...te was sent for sequence analysis, multiple alignment revealed single nucleotide substitution at the base pair number 121 from the start codon which give lead to single Amino Acid Substitution from CAG (Glutamine(Gln/Q)) which is considered as a polar Amino Acid to AAG (Lysine(Lys/K)) which is a basic Amino Acid, then the sequenced product of the local isolate was cloned Into PET 151 D topo plasmid with an N-terminal tag containing the V5 epitope and a 6xHis t...

Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1

...oteins based on sequence analysis and multiple alignment revealed single nucleotide substitution at the base pair number 121 from the start codon which give lead to single Amino Acid Substitution from CAG (Glutamine(Gln/Q)) which is considered as a polar Amino Acid to AAG (Lysine(Lys/K)) which is a basic Amino Acid which is very characteristic for the local isolate than the rest of isolate and strains.

...

A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3

...e from Saudi Arabia. The analysis showed that the highest nucleotide identities were 100% with other isolates that isolated from Saudi Arabia and was 98% with other isolates from Iran and Sudan. While the identity was 90% with all other members in the first cluster. The data refers to the isolated CYSDV virus in this study is more related to the isolates from Iran and Sudan. CP gene of CYSDV (CYSDV-CP) was cloned into the expression vector upon induction; the ...

Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2

...asis of the phylogenetic analysis of the HA gene sequences to study the genetic evolution of the NS1 gene of such viruses. The viruses were isolated from chickens and ducks in both live bird markets and backyard populations. NS1 gene was amplified, sequenced and the phylogenetic analysis of the obtained sequences revealed deletion of 5 amino acids at positions 80 to 84 in the NS1 protein along all Egyptian isolates under stu...

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

...al time RT-PCR; sequence analysis of partial S1 gene, cross-neutralization and the challenge experiment for H120 vaccinated broiler chickens were also performed. Results: Egypt/Qal/014p had close relation with that of the Massachusetts prototypes. Comparisons nucleotide and amino acid sequence of partial S1 gene showed that the recent isolate had 98.2% and 95.2% nucleotide similarities and 96.8% and 91.4% amino acid similarities with the commonly used IBV vacc...
Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2
...uencing and phylogenetic analysis of 6
representative samples were performed.
Results: Testing of 80 collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular
fluids by real time Reverse transcriptase polymerase chain reaction (rRT-PCR) revealed 65 out of 80
FMDV positive samples (81%).Typing of the highly viral loaded FMDV positive samples (40 out of
65) by conventional reverse tran...

Naglaa F.S. Awad1, Gamelat K.F. Kotb2

...T-PCR). Hemagglutination analysis of RRT-PCR positive samples were performed, also these
positive RRT-PCR samples were purified and directly partial sequenced for C-terminal VP60 gene.
Results: Only two samples were positive for partial C-terminal VP60 amplification. The first positive
sample was collected from non-vaccinated young rabbit (45-day old) and designed here as SHAH
2015, whereas the second sample was ...
Rasha M. Mahrous1,4, Ahmed K. El-Attar2, Ahlam A. AlWatban1, Sohair I. EL-Afifi3,
Nagwa M. Aref1,3
...653”. Phylogenetic analysis showed a high similarity (99%) with Oman isolate.
Conclusion: Phytoplasma strain of LWB-Eg Candidatus Phytoplasma that reported as a natural causal
agent from Lime WB strain in Egypt was classified as 16SrII subgroup.
...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
.../div>
phylogenetic analysis of the RPO30 gene and open reading frame (ORF) 103 genes. The sequences
designated Sheep pox virus was submitted to GenBank under accession number of MF443334.
Conclusion: The molecular techniques based on the RPO30 and ORF 103 genes are efficient for the
characterization and differentiation of Capripox viruses. This finding may provide new information on
the epidemiology of SPV ...

Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2

...quenced for phylogenetic analysis.
Results: FMDV RNA was detected in eight out of the 12 samples tested using real time RT-PCR.
Conventional PCR revealed that Six of the eight samples were positive using pan FMD primers.
However, using serotype-specific primers; only four samples tested positive; two samples for serotype
O, one sample for serotype A and one sample for serotype SAT2 while two samples remained
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
...ding to the phylogenetic analysis it falls into the classical group of Egyptian AI viruses
subclade 2.2.1.2 which is dominant in Egypt since 2012 till now. Therefore, the virus was identified as
HPAI according to the Intravenous Pathogenicity Index (IVPI) score (2.1). On the other hand, the
strain identity to some AI vaccine strains was determined for further characterization.
Conclusion: our experiments in this ...
Asma Kaouachi1, Mohcen Menaa1*, Abderraouf Chouaib Rebbah2 and Mohamed Cherif Maazi1
...on of digestive-contents analysis (86 contents) and field observations (during the breeding season). We identified 16 plant species of which four were recorded through on-site observations and 12 species were collected from bird crops. The biomass contained in the alimentary tract showed that fruits dominated the bird diet but the proportion of food items varied among seasons reflecting their seasonal availability in the study area. The pattern of seasonal var...
Ruqayya Bint Khalid1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Zubair Shabbir3 and Masroor Ellahi Babar4
...truction and statistical analysis, respectively. Characterization of physical and chemical properties of β-casein protein was performed by ProtParam and SWISS MODEL was utilized for protein model prediction. Sequencing results of amplified PCR products revealed total 9 SNPs including 1 in exonic and 8 in intronic regions of β-casein gene. Results represent the presence of A2 allele in milk of Cholistani Cattle of Pakistan. Multiple sequence alignment...
Abdullah Sheikh1,*, Faisal Almathen1,2 and Hairul Islam Mohamed Ibrahim3,4
...>TYR gene expression analysis from various dromedary or Arabian camel (Camelus dromedarius) phenotypes from Saudi Arabia. The four camel groups included were white, diluted (light brown, creamy and fawn), black and dark brown representing eumelanin and pheomelanin coat colors. Generally, the TYR gene expresses less in non-pigmented than the pigmented species. The results of our study showed that the mRNA from skin biopsies has low expression ...
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5
...nt origins. Phylogenetic analysis of local isolates showed a close homology with the isolates from Australia, Brazil, Turkey and Japan. It was found that aged, exotic and crossbreed cattle were more susceptible to A. marginale infection in summer season compared to the younger and indigenous cattle breeds, respectively (P˂0.01). Moreover, the study revealed widespread prevalence of A. marginale in the subtropical ...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

...Conclusion: phylogenetic analysis of LSDV at summer 2017 revealed that recent isolates has no
genetic difference compared by other isolates from Egypt or Africa during previous few years, as
LSDV affected sheep poxvirus (SPV) vaccinated cattle,
...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...uencing and phylogenetic analysis.
Results: The study revealed that 19% of our samples were positive to EHV-1 and phylogenetic
analysis showed that the Egyptian EHV-1 isolates were more than 99% similarity to European
abortogenic isolates (EHV-1 strains: Army 183 and Suffolk/48/2013). Indeed, the analysis reports that
these viruses are circul...
Othman N. O. Mansour 1, Naglaa Hagag 2, Momtaz A. Shahein 1, Sayed S. Hassan 1 Ahmed
A. EL-Sanousi 3 and Mohamed A. Shalaby3
...antibodies. Phylogenetic analysis of coronavirus ORF1a partial sequences in some positive
nasal swab samples of imported breeds (with accession number on gen bank; MH184776, MH184776
and MH184777) confirmed presence of different branch of MERS-CoV from which isolated in Middle
East.
Conclusion: MERS-CoV is amphixnoses in which both human and camels are source of infection and
cause mild to severe resp...

Ayman S. El-Habbaa1 and Mervat E. Radwan2

...in detection and genetic analysis of BEF virus among suspected cattle in
August 2017, Qualyubia, Egypt.
Methods: Partial sequence was generated after detection by reverse transcription-polymerase chain
reaction (RT-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating in this region. Sequence analysis of G-gene...
Allam A. Megahed, Badawi A. Othman, Samar S. El Masry, Abeer A. Faiesal and Mohamed A. Nasr Eldin
...>in size. Ultrastructure analysis showed different degrees of chloroplast degradation in PVM-infected
potato leaf tissues.
Conclusion: Occurrence of PVM was detected and confirmed in commercial potato seed tubers in
Egypt using the RT-PCR technique.
...

Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2

.../div>
phylogenetic analysis were performed for the AMV coat protein (CP) gene. An amplicon of the
predicted size (∼666 bp) derived from O. basilicum isolate was purified and cloned in E.Coli
into pCR®4-TOPO vector before proceeding to DNA sequencing and the alignment of
sequences.
Results: Electron microscope examination of negatively stained preparations from
symptomatic basil leaves re...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi
...rization. Also; sequence analysis of the same IBV-positive samples.
Results: flocks had shown high discrepancy of CV% values with regard to ELISA results against IB
virus. Out of 60 flocks tested; 12 were positive to the presence of IBV by rt-RT-PCR despite the fact
that the detected flocks had a history of previous IBV vaccination except for backyard chickens and
ducks. Sequence analysis...

Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi

...018 (H9N2). Phylogenetic analysis of the obtained sequence
cluster the characterized strain along with (formerly) G1-like viruses’ subtype B like those circulating
in the Middle East.
Conclusion: the AI H9N2 avian influenza virus is circulating causing major problem among chickens.
More studies need to be addressed to understand the real effect of the H9 virus on chickens and
humans in Lebanon.<...

Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2

...es. Also, a phylogenetic analysis based on the sequence of the hyper variable
region of VP2 gene was carried out.
Methods: Forty field bursa samples were collected from different localities. IBDV was detected using
RT-PCR. Sequence analysis of the variable region of VP2 gene purified from the PCR product.
Comparative phylogenetic analysis bas...

Shimaa M. Gad1, Ahmed A. Kheder1, Mohamed A. Awad 2

...uencing and phylogenetic analysis were performed to identify the
detected phytoplasmas.
Results: Phytoplasma was transmitted successfully from naturally infected gazania to healthy
ornamental plants by dodder and insect. Transmission electron microscopy (TEM) revealed that,
phytoplasma-like bodies were detected inside phloem, sieve tubes and parenchyma cells of leaf
midribs tissues in infected plants ...

Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2

...antitative real-time PCR analysis.
Results: Treatments with PGPF resulted in lower virus severity and titer than the control. GP17-2 treatment exhibited the lowest disease severity and virus titer in comparison with other treatments. Quantitative real-time PCR results showed significant increased expressions of pathogenesis related genes PR1 and PR2 relative to the control plants.
Conclusion: PGPF isolates of GP17-2, GF19-1 and SKT1 eli...

Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1

...anscription PCR (RT-PCR) analysis.
Results: Both of virus severity and titer were significantly reduced in treated plants in comparison with the control. All growth characters and yield of cucumber plants were increased in GU23-3 treated plants than the control. RT-PCR analysis revealed increased expressions of different pathogenesis related genes (Chitinase, β 1-3 Glucanase, PR1, POX, PAL and LOX) explainin...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3
...se mRNA and metagenomics analysis using MinIon technology.
Methods: A total of 34 adult female ticks were collected from three LSD clinically disease cattle that
were previously vaccinated using the Egyptian sheep pox (SPPV) vaccine, apparently healthy cattle in
contact with the diseased cattle, and one healthy animal located in a previously infected zone. LSDVspecific
PCR was used to detect LSDV in sampled ticks...

Samah A. Mokbel, Eman A. Ahmed, Hanan F. El-Kammar, Ahmed A. Kheder

...iv>Results: Phylogenetic analysis results indicated that SEA-CMV-isolate under study (acc. no.
MT491996) closely related (99.9%) to Beet-EG-CMV-isolate (JX826591), isolated from sugar beet
plants from Kafr El-Sheikh governorate, Egypt during 2012. Results of sequence analysis confirmed
the classification of isolate SEA as a member of group IA. Infection of sugar beet leaves with CMV
...

Gulnaz Ismaylova

...using geographic spatial analysis using the ArcGIS (Geographic Information System) program. 

...

Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski

..., aph-3-1 and aadE). The analysis in the third part of the study was concentrated on the antimicrobial resistance of the C. jejuni isolates towards three important antimicrobials (ciprofloxacin, erythromycin and tetracycline). The PCR method used revealed highest prevalence for Blaoxa-61 (25%), followed by CmeB and tet(O) genes (19.4%) and aadE with 13.9%. The aph-3-1 gene was not detected in none of the C. jejuni isolates. C. ...

Anila Kousar, Muhammad Naeem*, Samrah Masud, Abir Ishtiaq, Zara Naeem and Rabia Iqbal

...rometer was used for the analysis of whole-body elemental concentrations in relation to fish size under the influence of various dietary protein levels. The concentrations of K, Na, Ca, Mg, Fe, Zn, Pb and Cd were quantified for T1, T2 and T3 fish samples. An increasing trend was observed in the concentrations of studied elements with the increase of dietary crude protein in fish feed. The elemental concentration in the carcasses of studied GIFT yielded similar...

Nadeem Anwar1, Muhammad Luqman2*, Shaoib Nasir3, Moazzam Sabir1, Hafiz Bashir Ahmad4 and Saleem Ashraf5

... citrus cultivation. The analysis of Socio-economic characteristics of farmers sums up that the area under citrus cultivation is the most influencing characteristic that changes the marketing behavior of the farmers. Whereas the constraint analysis sums up that; large majority (83.6%) of farmers reported high price of inputs as a key hurdle. Majority of the respondents revealed their concern on rising inflation in the countr...

Muhammad Nasir1, Muhammad Usman Asif2*, Abdul Hayee Abid1, Qurat ul Ain Haneef1 and Muhammad Awais2

...nce. Multiple regression analysis was used to estimate the combined as well as individual impact of maximum and minimum temperature and relative humidity on population fluctuation of insect pests. The R2 values indicated that maximum and minimum temperature were the most influential factors and played a significant role in the population fluctuation of the studied insect pests as well as CLCuV. The results of the study may be helpful to the pest managers as a ...
Khawaja Adeel Tariq1*, Hadhi Hassan Khan2, Shuja Zaib Afghan3 and Arslan Raza4
...r the intersections. The analysis results of intersection with proposed modification shows improvement in Level of Service. 
...

Mian Ibad Ali Shah*, Yasir Saleem Afridi and Laiq Hasan

...hieved using descriptive analysis of the time-series data. The goal was to check the effect of various parameters (like new tests, government implemented lockdown index, etc.) on the new/ active COVID-19 cases, so policies can be devised accordingly to control the spread of the disease. The data was first converted into summary numbers, followed by carrying out the statistical analysis that yielded meaningful results. Descri...

Reema Zia* and Noor Paio Khan

...roducers to society. The analysis depicts Pakistan’s stronger position towards WTO trade liberalization in the rice sector. Pakistan would not need to change any of its policies and would benefit from trade liberalization due to the expansion of rice production when world prices of rice rise as a result of the reduction of support in other countries.

...

Iftikhar ud Din* and Yousaf Hayat

...gricultural experiments, analysis of variance (ANOVA) is the widely used statistical methods for assessing the differences among the means of more than two treatments by considering single trait independently. In reality these traits are not independent and appropriate techniques for analyzing multiple traits simultaneously, is the multivariate analysis of variance (MANOVA). This study deals with the illustration of MANOVA u...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...infertility, the hormone analysis and genetic determination of SRY gene as some of infertile human had smaller sized testis. Blood and semen samples were collected from clinically diagnosed 130 oligozoospermic males throughout D. I. Khan. The patients included had been married for 3 to 19 years, their wives had normal reproductive capabilities; the couples were living together for more than 3 years but fertilization had not occurred. Separation of sperm...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1,  Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...chicken by transcriptome analysis. Twenty specific pathogen-free (SPF) chickens were divided into two groups as control and treatment. The chickens of treatment group were inoculated with MDV, and blood samples of two groups were obtained at 14 and 22 days post-infection (dpi) for RNA extraction. Transcriptome analysis was performed to explore the immune responses of chicken erythrocytes to MDV infection at 14 dpi and 22 dpi...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
...ination. The statistical analysis exhibited a significantly higher (p< 0.05) prevalence rate in minced beef (28%) and lymph nodes (24%) as compared to the muscles (12%). The highest antimicrobial resistance was recorded against the antibiotics of penicillin group (88.5 to 93.7%), followed by the aminoglycosides (68.4 to 85.5%), tetracycline (73.9 to 78.6%), and quinolones (59.6 to 63.1%). While lowest resistance level was observed against the antibio...

Zain Ullah1*, Anwar Ali Shad1 and Saqib Ali2

... (3) using spectroscopic analysis, including mass, 1D, and 2D NMR data techniques. The antibacterial assays of the obtained six oil sub-fractions (H-1 to H-6) were accomplished against S. epidermis, E. coli, and S. aureus bacterial strains and the results ranged from 10-18 mm zone of inhibition. The GC-MS analysis of the six oil sub-fractions (H-1 to H-6) confirmed the existence of linoleic acid, palmitic acid, and oleic aci...

Sana Javeid* and Khalid Nawab

...g an interview schedule; analysis were carried out by using cross-tabulation, frequency counts, percentages through Statistical Package for Social Sciences (SPSS). To measure the poverty level of apple orchard growers, poverty score card was employed. In addition, bivariate analysis was used to examine relationship between various extension practices and poverty. Results of the study revealed that majority (60.1%) of the res...
Nianhong Huang, Yan Wu, Yuanyuan Li* and Jinhong Zhao*
... variation. Phylogenetic analysis based on the 18S rRNA gene analyzed in the present study together with other published Hepatozoon species showed that there is no significant difference in the geographical distribution and limited host specificity. This is the first time to report the infection of Hepatozoon sp. in Naja atra for the endemic species in Chinaand increases the number of known host species. Morphological and molecular ...

Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1

...nitrile. The qualitative analysis results showed that the two extracts of O. aristatus resulted from in vitro culture containing rosmarinic acid and sinensetin. When compared to the wild type, the chromatogram had a larger area than the wild type. This study provides new information regarding the secondary metabolite content of two O. aristatus from in vitro culture.

...
Syed Azmat Ali Shah1*, Asfandyar Ahmed2, Khan Shahzada3, Syed Muhammad Ali4, Akhtar Naeem Khan5 and Akhter Gul6
...a good relationship. The analysis results match very well with the experimental observations. For numerical modeling of the infill wall, a modified micro-modelling approach is selected where the head and bed joints are lumped into the bricks and an interface model was defined between these bricks representing the behavior of the mortar joint. Appropriate material models and damage criteria are employed for each mode of failure. A parametric study was then perf...

Miloudi Hilali1*, Hanae El Monfalouti1, Larbi El Hammari2, Farid Amar3, Zakaria Mennane4, Malika Haddam5, Nadia Maata6 and Badr Eddine Kartah1

...il, the physico-chemical analysis such as acidity, humidity, fatty acids and sterols was assayed. The results obtained the sample which extracted in the month of december contains a higher percentage of β-sitosterol (95.2%) and higher percentage of oleic acid C18:1 (75.4%). Time of the maturation is a factor that can significantly influence the quality of olive oil. Oleic acid, criterion of nutrition and quality of olive oil. Also, the amount of stearic a...
Shoaib Khan1, Sida Hussain2, Amir Naveed1*, Nazia Wali3, Zafar Ullah1, Muhammad Arif1, Muhammad Sadiq4 and Muhammad Asif5
...60kW. Sensitivity analysis of the designed machine is done by varying the hyper-parameters of the wind turbine generators. 
...

Hina Fatima1*, Bushra Yasmin2 and Lal Khan Almas3

...iciency and productivity analysis of bitter gourd-capsicum multiple cropping systems under tunnels in Punjab, Pakistan. Given the wide land fragmentation and high population pressure, this is the dire need of time to get farmers involved in advanced and intensive farming techniques to attain self-sufficiency in production. This study conducted the efficiency and productivity analysis by applying the parametric and non-parame...
Arshad Khan1, Mohammad Ihsan1, Ali Hazrat1*, Mohammad Nisar1, Muhammad Laiq1, Maryam Bibi1, Nasir Ali2, Ulfat Naz1, Muhammad Zakria1, Nausheen Nazir3, Adam Khan4 and Muhammad Asif Nawaz5
... 7.7 to 48g. Correlation analysis revealed that leaf width was strongly significant with leaf length (0.454**), 100 seed weight with leaf length (0.289**), seed width was also found strongly significant with seed length (0.246*), and 100 seed weight was found significant with seed length (0.236*). On the basis of Cluster analysis, all the genotypes were divided into two linkages and further divided into 7 sub clusters. Every...

Sajid Abdullah1*, Huma Naz2*, Zunera Abbas1, Uzma Nazir1, Mehrunisa Basharat1, Tanveer Ahmed3, Adnan Ahmad Qazi2

...gt;Cr>Cd. Statistical analysis revealed that different concentrations and duration of Pb, Cr and Cd have significant effect on the frequency of micro-nucleated erythrocytes. The inferences of this study will be helpful in monitoring aquatic ecosystems using fish biomarkers. 
 
Novelty Statement | The study is novel for detecting the genetic damage at cellular level of fish (Wallago attu) caused by heavy metals and industria...
Ahmet Arpacık
...i>. Based on the one-way analysis of variance, significant differences were identified in both hair length and diameter, medullary diameter and index, and hair root diameter values among the three species (P <0.05).
...
Zunaira Noreen* and Khawar Sultan
...e avian composition. PCA analysis showed four clusters based on the nature of habitat and species behavior. Agriculture land was found to be the highest (H’= 3.394) and landfill the lowest (H’= 1.414) in biodiversity. However, landfill site registered the most number of birds (>9000). Among twenty species recorded at the landfill, four species viz., house crow, common myna, grey-throated martin and bank myna, recorded >95% of the total...
Ewa Czerniawska-Piątkowska1, Iwona Szatkowska1, Daniel Zaborski2*, Wilhelm Grzesiak2, Sara Tabor-Osińska1, Małgorzata Wasielewska1Witold S. Proskura1, Wojciech Kruszyński3 and Edward Pawlina3
...otyping. A bioinformatic analysis of the P1 regulatory sequence was also carried out. Three genotypes (TT, CT and CC) were identified. The CT genotype was the most frequent (0.40, 0.50 and 0.52 in Jerseys, HO and RW, respectively). The frequencies of the CC genotype were 0.22, 0.28 and 0.31 in the RW, Jersey and HO breeds, respectively, whereas those of the TT genotype were 0.19, 0.26, and 0.32 for HO, RW, and Jerseys,...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
Hui-Ming Li1, Bi-Ze Yang1, Xue-Jun Jin1,2, Lin-Miao Li1, Hai-Ying Jiang1
Xiu-Juan Zhang1 and Jin-Ping Chen1*
...y utilized for parentage analysis in pangolins and that it has a significant application in selective breeding in endangered and valuable mammalian species.
...
Kang Chen1,2, Dekui He1, Chengzhi Ding3,4, Yintao Jia1 and Yifeng Chen1*
...nd a linear discriminant analysis were used for the SDs and NEFDs. The results of the linear discriminant analysis indicate that riverine otolith stocks had higher rates of successful classification compared with lacustrine otolith stocks, and stocks of otoliths from the Chargut Co and the Kyaring Co lakes had the highest rates of misclassification. Although a 70% classification success rate was produced by Bochu Tsangpo, th...
Aftab Raza Khan1, Azhar Abbas Khan2*, Javed Iqbal3, Arif Muhammad Khan4, Zeshan Hassan2, Hafiz Muhammad Aatif2 and Umbreen Shahzad2
...easons. Descriptive data analysis indicated a statistically significant value of p ˂ 0.05 between arthropod abundance and volume of arthropods in the diet of both adults and juveniles. Chi-square test (χ2) reflected a positive relationship between adult crop contents and different calendar months and stands. The data regarding breeding biology exhibited mean incubation period, clutch size and egg weight of 16.67±1.45, 4.0±0.58,...
Wanqiu Zhao1,2, Taoyan Yuan1, Li Chen2, Xue Du2, Weihu Chen4, Yan Fu1, Dong Niu3,* and Lizhi Lu2,*
...covery stage). Taxonomic analysis revealed that Firmicutes, Actinobacteria, and Proteobacteria were the three most abundant phyla in the duodenum of the laying geese. Cyanobacteria predominated in broody geese, followed by Firmicutes, Proteobacteria, and Spirochaetes. The microbial communities of the recovery geese were dominated by Spirochaetes, Firmicutes, Cyanobacteria, and Proteobacteria. Additionally, the microbial diversity and richness were significantl...
HuaBin Zhang1,2, YaSen Li1, Tao Pan1, Peng Yan1, En Li1, Hui Xue1 and XiaoBing Wu1,*
... by immunohistochemistry analysis. This study aims to access the possible effect of leptin in Chinese alligator. The results showed that immunoreactivity of leptin was observed in the adipocyte of white adipose tissue, the gastric gland of the stomach, the lamina propria of the intestine and the interstitial cell and seminiferous tubule of testis, leptin receptor staining was detected in the adipocyte of white adipose tissue, the gastric gland and submucosa of...
Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5
...50 genes. Bioinformatics analysis of flagellar assembly in S. typhimurium identified several conserved structural elements. In this study, FliI a flagellar protein required for flagellar assembly and involved in a specialized protein export pathway was cloned and overexpressed. ΔfliI mutant Salmonella strain was used to transform fliI overproducing plasmid pTrc99A by electroporation. Using vital dyes (Alexaflour 488), visualiz...
Yanhong Hu* and Linkai Cui
...lls and a bioinformatics analysis. The result of immunofluorescence assay showed EpFAR to be localized to the cytoplasmic region, and the observed fluorescence pattern was characteristic of endoplasmic reticulum (ER) proteins. Comparative analysis of the prediction results showed EpFAR was localized to the ER membrane. These results, combined with the localization of other FARs, suggested the importance of ER as a related su...
Chaochao Hu1,2, Xue Xu1, Wenjia Yao1, Wei Liu3, Deyun Tai1, Wan Chen4 and Qing Chang1*
...genetic and evolutionary analysis for wader, and improves our understanding of the evolutionary and taxonomic research within Charadriiformes.
...
Mengke Wang1, Shucheng Huang2, Cai Zhang1*, Haizhou Gong1
Shunan Cuan1, Shuaishuai Wang1, Qi Shao1, Wenhao Xu1, Sudan Meng1
Pengfei Li1, Yuqin Wang1 and Zijun Yang1
...tively. The histological analysis results by Oil Red O and TUNEL staining showed that lipid deposition and hepatocytes apoptosis in the MAG treated groups were significantly lower than that in the control group. Blood biochemical indexes also revealed that MAG supplementation significantly decreased the activity of alanine aminotransferase (ALT) (p<0.05), the content of total protein (TP), albumin (ALB) and globulin (GLB) (p<0.05). Furthermore, supplemen...
Jun Yan Bai*, Yu Chen, Qi Hang Hu, Zhi Hao Dong, Ying Lei, You Bing Yang, Shu Juan Zhao and You Zhi Pang
Jun Yan Bai*, Zhi Hao Dong, Shu Juan Zhao, Ying Lei, You Bing Yang, Heng Cao and Kun Peng Shi

 Yanqiang Zhou1, Lixin Wang1, Chunxiao Hao1, Xiuyun Li2, Shakeel Hussain1, Dongdong Shen1, Zhiwei Peng1, Qi`an Zhai1 and Zhijun Hou1,*

...chnique. The microscopic analysis, based on the morphology of oocysts, indicates that the present parasites are Capillaria sp. in blue wildebeest; Eimeria christenseni, E. alijevi, Trichuris sp. and one Strongy-type species in goats; Trichuris sp., Nematodirus sp., Moniezia sp., E. macusaniensis, another Eimeria sp. and another Strongy-type (different with goats) in alpacas. It was discovered that the inf...
Niaz Muhammad1, Samina Yasin1, Zunaira Fatima1, Noor ul Ain1, 
Muhammad Faizan2 and Sadaf Naz1*
...istan. Sanger sequencing analysis of FGFR3 exon 9 revealed that more than 90%cases had the c.1138G>A p.(Gly380Arg) variant. Our results suggest that c.1138G>A variant is the most common cause of achondroplasia in Pakistan, a finding which is similar to that reported for achondroplasia patients from other countries.
...
Yuli Andriani1*, Rintan Octaviana Julia1, Lintang Permata Sari Yuliadi1, Iskandar Iskandar1 and 
Yaya Rukayadi2
... quality. Kruskal-Wallis analysis was applied to analyzed the data of tail color. Data on the survival rate were analyzed using analysis of variance with the F test with 95 % level of confidence. The result showed that there was 9 076 mg kg−1 of carotenoid contained in BPLM. The inclusion of 12 % BPLM to commercial feed was able to enhance the color quality on the tail of the Swordtail fish, i.e average colo...

Ayesha Gul1, Mohammad Sayyar Khan1*, Mazhar Ullah1 and Iqbal Munir3

...ts revealed that invitro analysis of sugarcane calli against PEG may be a useful strategy for initial screening and selection of stress tolerant sugarcane lines to be used in the future breeding programs.
...

Hidayatullah1*, Sammia Mahroof1, Saleem Abid2, Naveeda Anjum3, Noor Habib4, Akhter Saeed5 and Muhammad Arshad Farooq1

...ts. Therefore, stability analysis of six chilli advance breeding lines; NARC Chilli-1, NARC Chilli-2, NARC Chilli-3, NARC Chilli-4, NARC Chilli-5, NARC Chilli-6 was carried out. F1 hybrid ‘Big Daddy’ was used as check. Plant material was figured out under Randomised complete Block Design with three replications and four locations; Chakwal, Faisalabad, Islamabad and Swat. Combined analysis of variance showed that ...

Muhammad Zubair Ishaq1, Umar Farooq1, Muhammad Asim Bhutta2*, Saghir Ahmad2, Amna Bibi2, Hafeez UR Rehman3, Umar Farooq3, Javaria Ashraf4 and Samaria Nisar4

....71 kg ha-1. Correlation analysis showed that seed cotton yield was positively correlated with the boll weight (0.738**) and number of bolls per plant (0.53**). Sowing dates in March, April, and May were grouped together in cluster no. 2, whereas sowing dates in June were grouped together in group 1. This study helps to optimize the sowing date to meet the climate changes and enhance the seed cotton yield.

...
Ahmad Wahyudi1*, Sujono Sujono2, Listiari Hendraningsih2, Ari Prima2, Zane Vincēviča-Gaile3 and 
Ivar Zekker4
Naveed Farah1*, Izhar Ahmad Khan1, Asif Ali Abro2, Jehanzeb Masood Cheema3 and Muhammad Luqman4*
...at first, a longitudinal analysis of Landsat imagery of land use was done through GIS and Remote Sensing for a time period of 2001-2016. The results of GIS analysis revealed that during the period 2001 to 2016, Faisalabad showed 24% increase in urban area and 23 % of this expansion consumed agricultural land. In Sahiwal, urban land has increased by 21 %, which encroached 13 % of agricultural land and 8 % bare land. Qualitati...

Usman Khalique*, Muhammad Zahid, Noor Fatima, Syed Jawad Ahmad Shah

...er tiller. Correlational analysis of S. graminum population with meteorological factors revealed that relative humidity (R.H) had significantly negative impact on aphid population. Whereas, temperature had significantly positive correlation with aphid population on NARC 2009 (r = 0.58Min. Temp., r = 0.7Max. Temp.). The data of Pakistani wheat varieties with least population density of aphid would be helpful to make varietal selections for future breeding progr...

Amir Muhammad Khan1, Laila Fayyaz2*, Raziuddin2, Sajid Ali1, Israr-ud-Din1, Sheraz Ahmad2, Haidar Ali1 and Ijaz Ahmad2

...ate and erucic acid. The analysis of variance (ANOVA) found significant variation among Brassica napus L. lines for all the studied morphological and seed quality traits. Furthermore, analysis of correlation revealed that 1000 seed weight was significantly correlated with primary branches on main stem, main raceme length, pods mainraceme-1, and pod length. Two traits erucic acid and pod length had significant correlation wit...
Aneela Hameed1, Faqir Muhammad Anjum2, Zia ur Rehman3, Saeed Akhtar1, Asim Faraz4*, Majid Hussain1 and Amir Ismail1
...ed milk. Minerals’ analysis of the milk samples were conducted and it was found that lactation stages have significant effect on minerals composition i.e. macro minerals (Na, Cl, K, Ca, Mg and P) and micro minerals (Zn and Cu) in milk. Oxytocin administration showed significant effect on milk minerals during various lactation stages as sodium, chloride and copper contents increased while potassium decreased. It was concluded that indiscriminate use of ox...
Muhammad Mansha1*, Sidra Yaqub1, Asma Abdul Latif2, Muhammad Wasim3 and Peter C. Thomson4
...tional child. The analysis of Her2/neu showed that its levels were higher in younger patients (≤ 42 years) than in older patients (0.884 vs 0.614 units, P= 0.049). The ROC curve indicated that the optimal test (maximum Se + Sp) occurred at HER-2 Neu values of = 0.615, where Se= 0.73 and Sp= 0.49. The AUC value for this test was 0.57, with 1 being a perfect test, and 0.5 being equivalent to random allocation. In brief, premature terminat...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...d report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. Haematological examination of the blood demonstrated a decrease in total erythrocytes, packed cell volume, haemoglo...
Nagarathinam Arunkumar*, Jainullabudeen Gulsar Banu, Nagarajan Gopalakrishnan and Arkalgud Hiriyannaiah Prakash

 

...dition, the growth curve analysis confirmed that lipase production is associated with the growth phase of the isolates. The efficacy of the lipase producing wax degrading bacterial isolates was tested against the nymphs and adults of Solenopsis Mealybug, Phenacoccus solenopsis Tinsley and the Striped Mealybug, Ferrisia virgata Cockerell infesting cotton. The results clearly indicated that the bacterial isolates A. lwoffii PSAD2 and A. b...
Liyun Chang1, Yingbin Chen1, Qian Kang1, JianhuaQin1,* and Zhiyong Liu2,*
..., followed by sequencing analysis showed that all tested cytokines were amplified. The PCR products exhibited consistent melting curves, suggesting reliable specificity of the cytokine primers. Compared with the control group, the expression levels of IFN-γ, TNF-α, IL-2, IL-4, IL-6 of infected group were all up-regulated, demonstrating an important role of cytokines in controlling C. parvum infection. Hence, these findings suggest that expre...
Muhammad Arslan Akbar1, Khalid Javed2, Asim Faraz3* and Abdul Waheed3
...s were more suitable for analysis of morphometric traits than that of simple correlation method. PCA was used to ascertain the important morphometric traits having greater variation and these traits can be helpful for improvement and in formulation of breeding plans and selection criteria for elite animals.
...

Kemi Funmilayo Omotesho1*, Philip Akintunde Fatodu1 and Toyin Benedict Ajibade2

Theophilus Miebi Gbigbi* and Rodney Akpoviri Isiorhovoja

...was 5 persons. Budgetary analysis showed a net return of ₦930,700.00 per annum and return on investment was 1.73 per naira. This means that exotic chicken production is profitable. The double log regression model result shows that cost of day old chicks, feed, drugs and vaccines, labour, shelter and equipment significantly influenced exotic chicken production. The resource use efficiency result infers that DOCs and drugs and vaccine were under-utilized while...

Farrukh Ilyas1, Durdana Qaiser Gillani2, Mudassar Yasin3, Muhammad Amjed Iqbal4, Iqbal Javed5*, Shahbaz Ahmad6 and Iftikhar Nabi7

Inam Ur Rahman1, Sanam Wagma Khattak2*, Muhammad Israr3 and Saima Hashim4

Nazeer Ahmed Lashari1*, Saghir Ahmed Sheikh2, Aijaz Hussain Soomro2 and ShamsuddinTunio3

...lon bag. The statistical analysis showed that there was substantial variance between the varieties. Culinary superiority parameter such as WAR was ranged between 2.3 (DR-82 and IR-6). Volume expansion ratio was 0.8 in DR-82 and 5.70 in IR-6 in cotton bag. Present study concludes that the moisture (%) was highly increased in coarse varieties, 1000-grain weight was higher in DR-82 at 3 months storage in all packaging. Water absorption and volume expansion ratio ...

Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1

...s, In silico restriction analysis was performed for each specie. Restriction endonuclease TfiI was selected for further analysis. Restriction fragment length polymorphism (RFLP) was performed at the same reaction conditions for all species. RFLP analysis produced species specific restriction profiles. Comparing these profiles, we have identified the species origin of meat unambiguously.&nb...

Murad Ali* and Said Wahab

... of Malakand for further analysis. The peroxide values (18.073 meq kg-1) were found significantly higher in oil medium, followed by ghee (11.96 meq kg-1) and then tallow (10.507-meq kg-1). Initial value in oil,value of peroxide was recorded as low as (5.05 meq kg-1),which significantly increased to (20.68 meq kg-1) in the 5thhour of frying. In addition, a higher acid value (1.499) recorded in ghee medium followed by oil (1.389) and then tallow (1.250), highest...

Sheikh Muhammad Azam1,2 and Muhammad Naeem1*

...Therefore, current study analysis was performed to analyze body composition of the studied fish. A total of 73 specimens of S. commersonnianus having different size, ranged 94.5 to 1183g of body weight and 20.5 to 56.9 cm in total length were randomly chosen from Arabian Sea Karachi, Sindh, Pakistan for analysis of proximate study. The mean percent values of different constituents were 73.95% water, 3.58% ash, 3.98% fat, 18....
Muhammad Zahid1, Aneela Zameer Durrani1, Muhammad Ijaz1, Khushi Muhammad2,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal3
...iomarkers. A comparative analysis of physiological biomarkers was also made. For this experimental study 30 BEF positive cattle at their first lactation were selected and equally divided into three groups A, B, and C. A fourth group “D” comprising 10 healthy cattle was made as positive control while group C was negative control. The animals in group A were treated with antibiotic (fluoreconical @ 0.08 mg Se/kg BW) and NSAID (phenylbutazone @ 8mg/kg...
Amin Arif1, Khizar Samiullah1*, Riffat Yasin2, Bilal Rasool1, Shakila Naz1, Xijun Ni3 and Saleem Akhtar1
...ological and comparative analysis, the collection is attributed to Giraffokeryx punjabiensis and Giraffa priscilla. Size variation in dentition is taxonomically important for vertebrate evolutionary point of view and this is the main reason to conduct this study at this specific site to add additional information about family Giraffidae. The fossil site has well exposed Chinji and Nagri Formation and has dated approximately 14.2-9.5 Ma. In this s...
Ishtiaq Ahmad1*, Abid Usman1, Khursheed Iqbal1 and Shams Ur Rehman2
... random-effect model for analysis. Multiple regression based methodology was developed to use fixed-effect model for both dependent variables Return on Assets and Tobin-Q variables to discover the association between Corporate Governance and Firm Performance. It is concluded that Board Size, Board Gender, Board Experience and firm size have significant the ROA and Chief Executive Duality, Board Size, Board Gender, Board Experience, and Firm size have significa...
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...cyte maturation. QRT-PCR analysis revealed that Gas6 mRNA was continuously expressed during the process of oocyte maturation, disappeared after parthenogenetic activation, re-appeared and elevated in the blastocysts stage. The following western blot analysis revealed a similar expression pattern with QRT-PCR and immunofluorescence. In conclusion, the expression pattern of Gas6 gene is closely related to oocytes matura...
Manar M. Farouk1*, Amal El-Molla1, Fayez A. Salib1 and Yousef A. Soliman2
...llae, and the phylogenic analysis of stn gene of the selected strains and the retrieved sequences from GenBank showed the relatedness of the isolated strains to the other strains isolated from different sources. Accordingly, the analysis of disease associated factors and the robust phylogeny findings provide valuable data that will be useful for implementation of preventive measures for salmonellosis in small ruminant...

Muhammad Usman Asif*, Raza Muhammad, Waseem Akbar, Mubasshir Sohail, Muhammad Awais and Javed Asgher Tariq

...018. Multiple regression analysis was used to estimate the combined effect of all weather factors on population fluctuation of pink bollworm. The R2 values indicated that weather factors cumulatively explained 76.4, 91.4 and 69.4% of variability in the abundance of pink bollworm in 2016, 2017 and 2018, respectively. The results of the study allow pest managers to focus treatment during the time of year when moths are most abundant.

...

Zhi Hao Dong1, Jun Yan Bai1*, Xiao Ping Jia2, Ying Lei1, Hui Cao1, You Bing Yang1 and Guang Lu Li1

Dilshad Ahmad1*, Muhammad Afzal2 and Asif Ali Abro3

... and employed regression analysis model to estimate credit impact on household income and livestock production. An economic technique of log-linear regression model was applied to identify the factors influencing dairy production and estimate household income difference from dairy production due to formal credit from Zarai Taraqiati Bank Limited (ZTBL). Estimates of the study indicated that agricultural credit facilitated to enhance household income 23.58%, in...
Nasir Ali Tauqir1, Asim Faraz2*, Muhammad Arif1, Abdul Rehman1Imtiaz Hussain1, Abdul Waheed2, Gabriele Marino3 and Michela Pugliese3

 

...istically. Hematological analysis indicated decreased (P<0.01) mean corpuscular volume and hemoglobin concentrations in buffaloes fed gradual decreased dietary EAA concentrations. It is concluded that the varying levels of limiting amino acids have effect on nutrient intake, utilization, milk yield and hematological parameters. These results could be used for further studies of this field.
...
Muhammad Arsalan1, Muhammad Aman Ullah1 and Abdul Waheed2,*
...8. The simple regression analysis shows that barrel depth is best predictor of live body weight.
...
He Yujiao1,2, Xie Huichun1, Lin Gonghua2,3, ZhangTongzuo2,3, Su Jianping2,3 and Du Yurong1*
... compared the clustering analysis results generated using mtDNA versus microsatellite markers. The most obvious difference between the two markers was reflected in the fact that the populations Yelashan (YLS) and Bangda (BD) represented a separate cluster based on the microsatellite data. Additionally, our studies involving the comparison of the two markers also reveal that a few of the differentiated populations from the middle region have a higher admixture....

Syeda Shazia Bokhari, Aisha Waheed Qurashi*, Roheela Yasmeen*, Fouzia Yasmeen, Nabeela Nayab, Uzma Rafi

... incubation. Qualitative analysis of exopolysaccharides (EPS) in congo red supplemented Brain Heart Infusion (BHI) agar medium showed positive results and EPS contents showed higher carbohydrate content as compared to Protein contents. It was found that the microbes associated with earthworm (L. terrestris) have significant potential of adherence and can be exploited as a bioinoculant formulation for improving soil fertility. 
 

Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4* 

...collected for laboratory analysis. Laboratory analysis including bacterial culture and biochemical tests were done to identify the causal agents of SCM and udder lesions. The overall prevalence of SCM and udder lesions were 56.8% and 32.1%, respectively. Most of the farms under study were small scale (94%) except a few were medium-scale (6%). Very few farms used quarantine and isolation shed (6%) for the disease affected cow...
Teuku Fadlon Haser1*, Muh. Saleh Nurdin2, Eddy Supriyono3, Deni Radona4, Fauziah Azmi1 , Kukuh Nirmala3, Widanarni3, Tri Heru Prihadi4, Tatag Budiardi3 and Riris Yuli Valentine5
...ity in Bogor for further analysis. Regression, chi-square, Mann Whitney test and t-test were applied to analyze reproductive biology parameters. There was no significant between-site difference in the reproductive parameters of mahseer from Atu Suasah and Lawe Melang Rivers. The sex ratio was balanced. The growth pattern was allometric negative and the Fulton condition remained stable over the six month study period. Mahseer in the 610-710 mm length class made...

Khlood G. Abdelkhalek*, Aly B. A. Badawy, Mohamed Fathi, Elshymaa A. Abdelnaby 

...pler examinations, image analysis, and blood sampling were performed on a weekly basis. Results showed that FSH level was elevated (P<0.05) firstly from 5-months to 6.5-months-old, and then a second elevation was reported at 9.5-months-old, both E2 and NOMs levels were increased significantly (P<0.05) from 8-months to 10-months-old. Testosterone level showed a significant (P<0.05) increase from 8.5-months to 10-months-old, while LH level was elevated ...
Longjun Guo*, Jiaqi Teng, Juan Wang and Yukun Chen
...nt tool for non-invasive analysis of the internal brain structure, is playing an increasingly important role in medical clinical and basic research.
...
Syed Sikandar Habib1*, Saira Naz2, Sadia Nawaz1, Iqra Ameer1, Ameer Khatoon1, Hameed Ur Rehman3, Sahibzada Muhammad Jawad4 and Haider Ali5
...Results of hematological analysis revealed that there is no significant (P > 0.05) difference in packed cell volume, mean cell volume and mean cell hemoglobin of different selected species adapted to similar environmental conditions. However, all other parameters like red blood cells, hemoglobin, mean cell hemoglobin concentration, white blood cells, lymphocytes, mid-range cells, granulocytes and other less important parameters including platelets, red cell...
Jarosław Pytlewski1, Ireneusz R. Antkowiak1 and Ewa Czerniawska-Piątkowska2*
...ng traits. The analysis confirmed significant statistical differences (P ≤ 0.05) between homozygotes (+,+) and heterozygotes (+,-) for the following traits: age at first calving (IVS5+438G>A), weights at weaning (c.1178G> A) and daily weight gain from birth to weaning (c.1178G>A). The most preferable results were found for homozygous (+,+) animals. The mutation in
Nabila Rasheed1*, Bushra Waseem1, Kanwal Haneef2, Shumaila Usman3, Madeeha Sadiq1, Tabinda Urooj1, Sarah Tarique2 and Dabeeran Zeha4
...gned for the comparative analysis of viability and healing properties of GDM affected WJMSCs (GDM-WJMSCs) with Normal controls (N- WJMSCs). Mesenchymal stromal cells were isolated from Wharton’s jelly of human umbilical cords from normal and gestational diabetic mothers. Cell viability was tested by trypan blue exclusion method and wound healing potential by scratch assay method. Healing of the scratch was observed at different time intervals (0h, 6h, 24...
Jhan Zeb1*, Saiqa Tufail1, Nausheen Saboohi1, Zafar Samuel2, Asher Azeem3, Yaseen Amir4 and Sehrish Akram5
...ed diets upon fatty acid analysis showed almost complete absence of linoleic acid, α-linolenic acid, ecosapentanoic acid (EPA) and docosahexanoic acid (DHA). However, these fatty acids were found to be present in the flesh of L. rohita at the end of the experiment. The sum of saturated fatty acids (SFA) was higher as compared to the sum of monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) in L. rohita flesh. Regardless...

Vladimir Safonov 

...nvestigated. Biochemical analysis of the blood was also performed to estimate the functional state of the cow’s organism. In animals from the selenium-deficient region, the microelement content did not exceed 44.2–81.9% of the level in animals from the satisfactory region throughout all studied groups. Besides, an increased accumulation of lipid peroxidation products was observed. In particular, malonic dialdehyde increased by 17.9–96.6%. Als...
Xiaoyun He1, Yongfu La2, Jinxin Wang1, Ran Di1, Qiuyue Liu1, Xiangyu Wang1, Wenping Hu1 and Mingxing Chu1*

...he results of haplotypes analysis indicated that H2, H7 were preponderant haplotype in high prolificacy sheep breed. The litter size of TT genotype was significantly higher than that in CC genotype at T1235C loci in STH sheep (P<0.05). The current study provides evidence in sheep for genetic markers that might be used in breeding program. 
...

Muhammad Waryam Warraich1, Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Fakhar Mujeeb3, Muhammad Zeeshan Maznoor1, Aneela Riaz4 and Sarfraz Hussain2

... carried out followed by analysis in the laboratory. It was observed that use of press mud @ Yield 1 % in integration with SOP as chemical K fertilizer performed as best treatment by improving yield (4.11 t/ha) and yield contributing parameters like maximum plant’s height (95.10 cm), length of spike (11.04), number of fertile tillers (5.67), 1000 grains weight (39.26 g) and grain count per spike (51).

...

Vahid Mollasadeghi1,2* and Samaneh Elyasi1,2

...rs Agria. The results of analysis of variance of the evaluated traits showed that the effect of PVY virus treatment was significant on chlorophyll a, b, leaf area and stem length at 1% and 5%, respectively. In addition, the effect of Pars Humic seven on chlorophyll a, b, leaf area and stem length was significant at 1% level. The results of the interaction effect of PVY virus treatment in Pars Humic were significant only for chlorophyll a, b and leaf area at 1%...
Samaria Nisar1, Tariq Manzoor Khan1, Muhammad Ahsan Iqbal1, Rahmat Ullah2, Muhammad Asim Bhutta3*, Saghir Ahmad3, Amna Bibi3, Hafeez ur Rehman4, Umar Farooq4 and Muhammad Zubair Ishaq5
...tantial variation in the analysis of variance (FS). The goal of this research was to quantify variability and conduct a correlation analysis to see potential for selection in the F2 population. Plant height (cm) was found to have a positive relationship with boll weight, bolls/plant, fiber strength, fiber length, and fiber fineness when the correlation coefficient was calculated. The bolls/ plant revealed a positive correlat...
Aiguo Zhou1,2,4, Shaolin Xie1,2, Zhuolin Sun1,2, Yongyong Feng1,2, Shulin Liu1,2, Di Sun1,2, Yanfeng Chen3 and Jixing Zou1,2*
... performed morphological analysis among five Channa species. The principal component analysis showed that the cumulative contribution rate of five principal components reached 78.928%, and tail shank (TS) and head length (HL) were the main contributors to the first principal component (35.435% contribution rate). The scatter diagram of the principal component analysis showed that wh...
Ali Ikhwan1*, Dian Indratmi1, Faridlotul Hasanah1, Manar Fayiz Mousa Atoum2,3 and Irum Iqrar4,5
...entrifuge. The metabolic analysis was performed using Gas Chromatography-Mass Spectrometry (GC-MS) and comparing the data with the National Center for Biotechnology Information (NCBI) database to obtain specific metabolites. The inhibition test was done with one control (P0: chemical fungicide) and two-level of treatment, i.e., the concentration of purple Cleome extract (P1: 80 % and P2: 40 %), then ANOVA and HSD test were carried out to find out the best trea...

Prima Mei Widiyanti1*, Mirnawati B Sudarwanto2, Etih Sudarnika2, Raphaella Widiastuti1 

...nd application of hazard analysis and a critical control point (HACCP) in the food industry.

Keywords | Antibiotic, Enrofloxacin, Heating, HPLC, Milk 

...

Abbas M. Al-Azab1*, Ahmed A. Zaituon2, Khalid M. Al-Ghamdi3, Fahd M. Abd Al Galil4,5 

...The obtained statistical analysis showed that the availability of the adult and juvenile stages of A. aegypti occurred throughout the year in different densities depending on the climatic conditions and the effectiveness of the breeding sites. The density of adult were 2.61 (Ghuleel), 2.55 (Al-Jameiah), 2.25 (Al-Balad), 2.47 (Al-Safa) and 1.86 (Al-Nazlah Al -Yamaneyyah). Whereas the density of immature stages were 5.03 (Ghuleel), 4.01 (Al-Balad), 2.48 (Al-Jame...

Nedžad Hadžiomerović1*, Ozan Gündemir2, Senad Kovačević1 

...&H. The morphometric analysis was performed on the 11 measurements and geometric analysis evaluates shape variation between two species with ten landmarks. The study revealed that the mandible of the jackal was longer and of massive bone compared to fox mandibles. The most significant difference was the total length, the indentation between the condylar and the angular process, and the measurement from the aboral alveolu...
Tabinda Urooj1,*, Bushra Wasim1, Shamim Mushtaq2, Syed Nudrat Nawaid Shah1, Lubna Faisal3, Moazzam Ali2, Nabeela Rasheed1 and Syed Faizan Ali Rizvi4

Imran Ali Chandio1, Muhammad Nawaz Kandhro1*, Qamaruddin Jogi1, Ghulam Murtaza Jamro2 and Siraj Ahmed Channa3

...HO-1 and Hysun-39). Data analysis revealed nitrogen fertigation, genotypes and their interaction substantially (P≤0.05) affected growth and yield traits of sunflower. Greater seed yield (kg ha-1) and the oil content (%) were documented in N10 = 125 kg N ha­­­­­-1: three splits (⅓ broadcasting at sowing time + fertigation- ⅓ at 1st and ⅓ at 3rd irrigation), followed by N8 = 125 kg N ha­­­­­-1: two splits (½...

Muhammad Iqbal Jakhro1*, Nadeem Sadiq1, Javed Ahmed Abro1, Amanullah1, Fateh Muhammad2, Maqbool Ahmed3, Syed Ishtiaq Ahmed Shah3 and Qasid Hussain4

...stem. As regards to soil analysis for nutrients concentration, it is concluded from this study that the observed from various of parameters which is growth and developments of olive saplings and soil nutrient status improved through mineral fertilizer when applied in soil during the two periods (three months), besides treatment 5 (NPK 50:25:25) was the superior in this concern.

...

Ali Zohaib*, Muzzammil Hussain, Iftikhar Ahmad and Adnan Bashir

...110 g per tray. Economic analysis exhibited that maximum net returns and benefit cost ratio (BCR) was achieved by using 90 g seed per tray. In conclusion, 90 g seed per tray improved the grain yield by enhancing number of productive tillers and grain weight of fine basmati rice; hence, could be adopted for better productivity and economic benefits of mechanically transplanted fine basmati rice.
...

Muhammad Rizwan1*, Jehanzeb Farooq1, Amjad Farooq1, Muhammad Farooq1,2, Ghulam Sarwar1, Muhammad Nadeem1, Muhammad Riaz4, Muhammad Rafique Shahid3, Hafiz Ghazanfar Abbas1 and Muhammad Kashif Shahzad Sarwar1

...ults were noticed in the analysis of variance. Further investigation in correlation analysis revealed that there was comendable positive association among leaf chlorophyll contents, seed cotton yield (SCY), No. of sympodia, seed index and boll weight(g). Principal Component Analysis (PCA) demonstrated that only 02 out of 08 PCs showed eigen value greater than 1. The contribution of first t...

Rehman Shahzad1, Saba Irshad1* and Faisal Amin2

...ed to perform mutational analysis. Phylogenetic analysis revealed that B2 sub-lineage is endemic in the country as all isolated H9N2 strains belongs to this clade of G-1 lineage. Two isolated H9N2 strains A/Chick/Pak/303/2018 and A/Chick/Pak/436/2019 contain six glycosylation sites instead of seven as found in their ancestral strains. A comparative analysis of neuraminidase amino-acids seq...

Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2 

...ugh which a phylogenetic analysis was conducted for six isolates showing a high degree of similarity between them and those imported from Genbank. hilA, spvC, stn, and qacED1 genes were detected in 75, 16.67, 66.67, and 50% of Salmonella serotypes, respectively. These findings signify the role played by chicken-ready meals, as well as their handling, in the high rate of multidrug-resistant Salmonella isolates and the risks it poses to public health.

Ke...

Saleem Ur Rahman1, Muhammad Irfan Yousaf1*, Mozammil Hussain2, Khadim Hussain1, Shahid Hussain1, Muhammad Husnain Bhatti1, Dilbar Hussain1, Aamir Ghani1, Abdul Razaq1, Muhammad Akram1, Iqra Ibrar1, Muhammad Shakeel Ahmad1, Shoaib Anwar Kohli3, Muhammad Abubakar Siddiq4

... conditions. Correlation analysis indicated that some high temperature stress indices i.e., STI, MP, GMP and HARM had a strong positive correlation with kernel yield under normal (Yp) and high temperature stress conditions (Ys). Biplot analysis further unveiled that two local maize hybrids i.e., YH-5507 and YH-5427 were the most productive, stable and heat tolerant while YH-5532, P-1543 and NK-8711 showed poor performance un...

Ho Shi Hui1, Eng-Keng Seow2, Nurul Huda1* 

...ture and texture profile analysis were compared. Ultrasound treatment had caused a significant (p<0.05) change in pH, foaming capacity, and the folding test score of egg white gel. However, texture profile analysis showed that the hardness, springiness, gumminess and chewiness of egg white gel had decreased after ultrasound treatment. Ultrasound-treated duck egg white for 40 minutes showed a significantly (p<0.05) high...

Rehman Shahzad1, Saba Irshad1*, Malik Saddique Mehmood1 and Faisal Amin2

...gment. Phylogenetic tree analysis showed sequences from 2015 to 2017 form a single evolving clade. Valdar residues conservation scores by multiple sequence alignment showed the C terminal region of NEP protein is conserved while C terminal region of effector domain (ED) of NSI protein exhibit mutations. These mutations are enhancing the total hydrophobicity of the molecules. Hydrophobicity was calculated by using Kyte and Doolittle method. High hydrophobicity ...

Tintin Rostini1*, Irwan Zakir1, Danang Biyatmoko2 

...ined were analyzed using analysis of variance while differences between treatments were further analyzed with Duncan’s test. The results showed that yeast + curcuma (TR3) supplementation had a significant performance by increasing consumption, dry matter digestibility, organic matter, crude protein, as well as neutral detergent (NDF), and acidic detergent fiber (ADF), average daily body weight gain, feed conversion, and blood metabolic profile (total pro...
Lin Ye, Jie-Lan Jiang*, Jia Xu, Rui-Ting Liu, Yin-Qiu Tian, Qing-Qing Zang, Jia-Yi Cao and Ming-Guang Mao*
...vidus. SignalP and TMHMM analysis showed that TrCLCN5-deduced protein was a type III transmembrane protein and lacked a typical signal peptide. Conserved Domain analysis revealed that there was a Voltage_CLC domain and two CBS domains located in the TrCLCN5 deduced protein. qPCR analysis showed that TrCLCN5 was highly expressed in the intestine, kidney and liver, and up-regulated in gills ...

Muneeb M. Musthafa1,* and Fauziah Abdullah1,2,3

...ough with some diversity analysis. The Margalef index for Gunung Besar Hantu, Fraser’s Hill and Gunung Angsi showed 9.210, 7.214 and 6.777 respectively while Shannon-Weaner index ranged between 2.546 to 3.083. Evenness is very high at Gunung Angsi (0.930) and Fraser’s hill showed moderate level (0.51). Simpson diversity index is ranging from 0.859 to 0.946 while Shannon Weiner diversity index showed very close values for Gunung Besar Hantu and Gunu...

Khadim Hussain Memon1,*, Amir Mahmood Memon2, Tahira Jabeen Ursani3, Abdul Manan Shaikh1 and Sapna Waheed Memon1

...t being over-fished. The analysis of current exploitation rate E= 0.384/year was also indicative the sustainable fishery stock in the Indus River as it was lower than current fishing mortality.

...

Kashifa Naghma Waheed*, Tariq Rashid, Sikender Hayat, Muhammad Azeem and Sajid Ali Naqvi

...ubjected to Multivariate analysis of variance (MANOVA) to find out the statistically significant difference (p<0.01) between two treatments for their response to fish growth. The post-hoc test revealed that growth of Major carps was significantly higher with greenhouse as compared to without greenhouse (at p < 0.05). Growth and survival rate of Catla catla were found more pronounced as compared to the other two carps under greenhouse cover. The results f...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

..., SNAP cPL and blood gas analysis suggested that the dog have pancreatitis and chronic compensatory alkalosis. The X-ray examination revealed pleural effusion of the dog; B-ultrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary dia...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

..., SNAP cPL and blood gas analysis suggested that the dog have pancreatitis and chronic compensatory alkalosis. The X-ray examination revealed pleural effusion of the dog; B-ultrasound showed nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary dia...

Yin-Hua Wang1,2, Yi-Bing Zhu1, Li Jiang1, Bin Du3 and Xiu-Ming Xi1*

...to the study drugs. Data analysis will adopt an intention-to-treat approach. This trial will demonstrate the probability that remifentanil can reduce the duration of MV in long-term ventilated patients in critically ill patients compared to fentanyl.

...

Masouras P.K.1, Nikolaou K.1,2, Laliotis G.P.1*, Koutsouli P.1, Bizelis I.1 

...estimated using an image analysis approach. Samples of Greek Red had higher a* value (P<0.05) compared to those obtained from crossbreds. Female samples revealed higher (P<0.05) values of L* compared to male. Significant correlations were observed between some of the determined meat quality parameters. A strong correlation (r = 0.798; P<0.01) was noted between IMF and marbling content. The regression analysis betwee...

Abdul Haleem1*, Ghulam Hassan1, Arshad Iqbal2, Fahim Ullah Khan3, Muhammad Sajid3, Farhad Ahmad4, Rafi Ullah Khan5 and Mohammad Ilyas2

Kubilay Ucar1, Görkem Oruk2 and Sait Engindeniz1*

Altaf Mahmood1*, Muhammad Athar Khan2, Saima Parveen3, Tanveer Hussain4 and Ayesha Azad5

Seble Mulugeta1 and Amenu Leta2*
...logit model were used to analysis the data . The descriptive result revealed that a total of samples 37 (17.6%) were adopter of modern energy sources, 50(23.8%) were adopter of both traditional and renewable energy technology, and 123 (58.6%) were user of traditional energy source. The binary logit model revealed that age, family size, education, income, number of livestock owned, land size holding, and training were significant to adopt technologies. The mult...

Syukriah Syukriah1, Ulinnuha Nur Faizah2, Hendry T.S. Saragih3*, Rizki Fitrawan Yuneldi4, Soenarwan Hery Poerwanto5, Raden Roro Upiek Ngesti Wibawaning Astuti5, Stephan Immenschuh6 

...ng data the histological analysis of the mother’s and offspring’s liver and spleen were prepared by using the paraffin method. There were sigificant results in the parasites levels and the increase in P. berghei infection followed an increase of oxidative stress in mothers and offspring mouse in the treatment and control groups. The liver and spleen of mothers have been affected with P. berghei infection, however, there are still no effects on the ...

Seon-Ho Kim1, Mahfuzul Islam1,2, A-Rang Son1, Sang-Suk Lee1* 

....05). From Metataxonomic analysis, Bacteroidetes and Firmicutes were the most abundant bacterial phyla in both breeds and diets. Bacteroidetes was more abundant in the steers received HF diet while Firmicutes was increased with the HC diet. All four groups of steers had a distinct bacterial community with higher relative abundances. The principal component analysis (PCA) represented that the overall rumen bacterial community...

Muhammad Abdullah1, Sana Zahoor1*, Akhtar Ali2 and Masroor Ellahi Babar3

...r -238 and -308 genotype analysis by the ARMS technique and statistical analysis showed that there was association between asthma and TNF-α polymorphism at -238 G>A (p < 0.0001, OR 0.48) and -308 G>A (p < 0.0036, OR 0.26). 

...

Yao Zou, Shien Ren, Miao Xu, Nannan Liang, Xuxin Zhang, Chongxuan Han and Xiaoning Nan

... variation using one-way analysis of variance and cluster analysis in four species of zokor including Eospalax cansus, Eospalax rothschildi, Eospalax baileyi, and Myospalax aspalax. We also used principal component analysis and dichotomy to explore key measurements which could reflect skull differences to the maximum. In addition, we tested the correlations between environmental factors an...
Syed Makhdoom Hussain1,*, Hina Gohar1, Muhammad Asrar1, Muhammad Mudassar Shahzad2, Azhar Rasul1, Majid Hussain3, Muhammad Zubair ul Hassan Arsalan1, Nisar Ahmad4 and Aqsa Sharif1
...n of minerals, proximate analysis and hematology were determined using standard methods and formulae. Highest minerals absorption (Ca, Na, K, Fe, Cu, P, Mg and Al) was observed in the fish fed at 400mg/kg of polyphenols in canola meal based diets. Similarly, best hematological parameters (RBCs, WBCs, PLT, Hb, PCV, MCHC, MCH and MCV) as well as proximate composition (crude protein, crude fat, ash, moisture and carbohydrates) were noted in fish group fed on 400m...

Shuangye Wang1,2, Yunlin Zhao1,2, Zhenggang Xu2,3,*, Junzhi Chen3, Guiyan Yang3, Song Wang2 and Kangkang Jiang2

 

...lculated and the overlay analysis with seasonal home range data was employed. The results showed: (1) in seasons, the home range size of winter was the largest while it was the smallest in summer. It was also found that home range of dry and wet periods in 2017 was larger than in 2016 for the same period; (2) The milu population lived together in the released area. They even moved to outside of Dongting Lake in summer and moved back in autumn with the water le...
Damat Damat1*, Roy Hendroko Setyobudi2, Juris Burlakovs3, Zane Vincēviča-Gaile4
Devi Dwi Siskawardani1, Rista Anggriani1 and Anas Tain5
...ation variables included analysis of water content, ash, carbohydrate, protein, fat, antioxidant activity, fiber content, water absorption index, color intensity, and sensory evaluation. The data were analyzed by ANOVA and continued according to Duncan’s multiple range test. The results showed that all formulas of analog rice produced from arrowroot starch, with the addition of seaweed pulp and spices, fulfill the chemical and physical properties of padd...

Raheela Naz1*, Muhammad Aftab1, Ghulam Sarwar2, Ana Aslam1, Qudsia Nazir1, Asifa Naz3, Abid Niaz4, Farah Rasheed1, Amina Kalsom1, Nisa Mukhtar1, Sadia Sultana1, Ifra Saleem1, Arfan ul Haq1, Muhammad Arif1, Aamer Sattar1, Sarfraz Hussain5 and Muhammad Adnan Rafique6

...urthermore, the chemical analysis showed the maximum quantity of N, P and K in wheat grains obtained from T6. This study concluded that split application of fertilizer at various stages of wheat crop produced better yield as well as wheat quality. The joined addition of NPK at sowing time along with 2% spray of N and K after 30 and 45 days of sowing is the best approach for the increase in wheat growth and yield.
...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...long with haematological analysis, include the replicative and expressive activity of FeLV evaluation in the infected organism cells, innate immune factors for antivirals evaluation, interferon system (IFN) in particular, molecular guard factors for cell genome integrity evaluation (p53, etc.). Methods. In domestic cats with a previously established diagnosis of «FeLV-infection and chronic leukaemia» (n = 10) and healthy control cats (n = 6), we ex...

Noura El-Shahat Attia1*, Abd El-Khalek Ramadan El-Sheikh1, Mohamed Omia Siam2 

... taken for hematological analysis, along with taking serum samples for biochemical analysis. Rectal temperature, heart, and respiration rates were not significantly differed between the two groups. Packed cell volume (PCV), total erythrothetic count (TEC), white blood cells (WBCs), serum glucose, total proteins and serum Zn were significantly decreased. Moreover, while copper (Cu) and iron (Fe) were significantly increased (...

Ahmad-Ur-Rahman Saljoqi1, Sumayya Amin1, Muhammad Salim1*, Taufiq Nawaz2 and Farida Anjum3

...tify;">Pesticide residue analysis experiment of three different pesticides used against Helicoverpa armigera was conducted in tomato crop during 2019. The experiment was carried out in (RCBD) Randomized Complete Block Design. There were four treatments i.e., Cypermethrin, Imidacloprid, Lamda cyhalothirn and control. Each treatment was replicated three times. Lowest mean percent fruits infestation (2.84%), highest yield per treatment (9.56 kg) per plot, highest...

Saima Parveen1, Altaf Mahmood2*, Ayesha Azad3, Sajid Umar4, Nosheen Shoukat6, Mirza Muhammad Arsalan Azam5, Qurat-Ul-Ain5 and Nausheen Akhtar Malik1

...ected for collection and analysis of disease data. Five years’ data of these randomly selected farms revealed highest (44.64%) crude morbidity during monsoon season followed by 23.92%, 22.12% and 17.49% for winter, spring and post-monsoon seasons respectively. The highest (14.90%) prevalence was recorded for new castle disease followed by infectious bursal disease (11.79%), pullorum disease (11.17%), colibacillosis (8.71%), infectious bronchitis (7.87%),...

Javed Khan1, Abdul Majid1, Mohammad Nisar2*, Ali Hazrat2, Nausheen Nazir3, Muhammad Zahoor3, Mohammad Ihsan2, Azhar Hussain Shah1, Muhamad Ajmal Khan4 and Muhammad Yahya1

...ograms. Based on cluster analysis all the genotypes were divided into two main lineages; lineage A which is further divided into 3 clusters (C1, C2, and C3), and lineage B, further divided into 2 clusters (C4 and C5). The principal component analysis estimated the total variations in the range of 51.43 to 100% with an Eigenvalue of 0.25. It was concluded from the results that Alnus nitida L. genotypes available in Pakistan h...

Zhang Dong-jie1, He Xin-miao1,2, Wang Wen-tao1,2 and Liu Di1,2*

...s were sequenced and the analysis yielded 8,988,338 non-redundant SNPs plus 1,231,680 InDels. A phylogenetic tree was constructed and a principal component analysis (PCA) was performed based on previously published SNP data from 66 individual pigs. Both analyses indicated the Min pig fell between the European and Asian pigs, while the Northeast wild boar was closely related to the Asian domestic and wild pig breeds. Selectiv...

Muhammad Niamatullah1, Abdur Rehman1* and Raheel Saqib2

...ng procedure. Regression analysis as a statistical method was used to assess the impact of tube well irrigation on agriculture production, household annual income, poverty line, kinds of land, sources of irrigation and inputs used on land. The significant (P<0.05) impact of household annual income of farmers was observed on agriculture production. The significant (P<0.01) impact of household annual income of farmers had kept farming community above pover...
Hadia Gul1, Abdul Haleem Shah1, Ricardo Harripaul2, Anna Mikhailov2, Ejaz Ullah Khan3, Wasim Shah3, Nisar Ahmad3, John B Vincent2,4 and Muzammil Ahmad Khan3*
...y from Pakistan. Genetic analysis was performed through microarray genotyping and homozygosity-by-descent (HBD) mapping, whole exome sequencing (for mutation identification) and Sanger sequencing (for variant segregation). Homozygosity analysis revealed a 1.2 Mb HBD region on chromosome 15 between markers rs4778147 to rs8036234 (chr15:27752745-28962131bp), which harbors the previously reported OCA2 gene. The subsequent whole...
Anjum Ara1, Usman Ali1, Muhammad Furqan2*, Zulfiqar Ali2
Muhammad Mudassar Shahzad3, Khawaja Basharat Ahmed4
Riaz Aziz Minhas4, Waqar Ahmed2, Zakir Hussain2 and Mian Aman Ullah2

Hafiz Ghulam Muhu-Din Ahmed1, Aziz Ullah2, Muhammad Asim Bhutta3*, Amna Bibi3, Hafeez-ur-Rehman4 and Umar Farooq4

...utes. Results from radar analysis, performance designated that the root related attributes were the most sensitive and important attribute followed by shoot related attributes. Chakwal-50 wheat variety had highest dry root weight (1.256g), dry shoot weight (0.41g) and fresh shoot weight (2.83g), while had minimum shoot length (10.87cm). The genotype Ass-11 possessed highest fresh root weight (4.93g) while the genotype Pasban-90 shown maximum value for root len...

Hussain Ali1, Shahid Sattar Khan1, Fazal Maula2, Said Hussain Shah3* and Misbah Uddin1

...d in JP-5 variety. Yield analysis showed that maximum yield (2780 kg ha -1) was recorded in the Fakher Malakand variety. Data regarding the efficacy of different insecticides showed that maximum percent biological efficacy against rice stem borer (52.21%) was observed in Lambda-cyhalothrin, while minimum percent biological efficacy against rice stem borer (14.91) was recorded in Lufenuron. In case of the insecticides, minimum yield (1790 kg ha-1) was recorded ...

Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Kaiser Latif Cheema2, Abdul Ghaffar3, Imtiaz Ali4, Muhammad Jahanzaib Khalid2 and Zeshan Ali5

...ics, principle component analysis and cluster analysis were employed to detect the most genetically variable and high yielding chickpea genotypes. D2 statistics extracted higher values for standard deviation and coefficient of variation indicating that the studied genotypes possess considerable amount of genetic variation in performance of studied different traits. Principle component analysis...
Abdul Jabbar1, Muhammad Tariq1*, Asim Gulzar1, Tariq Mukhtar2 and Tayyaba Zainab3
...through Probit and Logit analysis (POLO) software. All the plant extracts and their green synthesized AgNPs caused the maximum mortalities of 3rd and 4th larval instars of C. pipiens after 96 hours. With the increase in time intervals and concentrations, the mortalities increased showing direct positive relationships between mortalities and time intervals and concentrations. The lethal and sub lethal effects of plant extracts on the mean development of larvae ...

Lifang Zhang, Xiuyue Zhang and Bisong Yue*

...een 0.602 and 0.923. The analysis of Genepop and Popgene showed that a relatively high level of genetic diversity was revealed: the observed number of alleles was 10.0781; the effective number of alleles was 5.2746. The observed heterozygosity was 0.6882, and the expected heterozygosity was 0.7791, Nei’s expected heterozygosity was 0.7661, Shannon’s information index was 1.8056. Most loci were found to be deviated from HW test. A higher level of ge...

Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1* 

...42%. The bacteriological analysis was carried out using conventional cultural and molecular means (cyclic polymerase chain reaction; cPCR) targeting the invA gene. The isolated Salmonella culture revealed higher resistance incidence up to 100% against amoxicillin-clavulanic acid (AMC; 30 μg), ampicillin (AMP; 10 μg), and nalidixic acid (NAL; 30 μg), 90% against enrofloxacin (ENR; 5 μg), and 80% against doxycycline HCL (DO; 30 μg). The convention...

 Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4

...oncentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiated osteoblast cells derived from isolated rBM-MSCs by flowcytometric analysis was studied by histochemical staining with Alizarin Red and von Kossa to confirm the osteogenic differentiation process. Also, cell viability assay by MTT was used to measure the number of viable osteoblast cells cultured with scaffolds extracts. Also, the antitum...

Xuelong Chen1, Honghai Wang2, Wenchao Li1, Weina Guo1 and Yanping Qi1*

...ducted a systematic meta-analysis aimed at exploring BHV-1 prevalence in yaks of the Chinese Qinghai-Tibetan Plateau (QTP). To complete this analysis, we identified all relevant articles on this topic in both English and Chinese literature databases published through March 20, 2020. Studies were not included in this analysis if they were reviews, repeats of previous studies, included incon...

Shahid Sherzada1,2*, Muhammad Naeem Khan1 and Masroor Ellahi Babar2

...t study aimed at genetic analysis of by Cirrhinus mrigala using mitochondrial DNA marker cytochrome oxidase I (COI). Genomic DNA isolated from whole blood was used to amplify and sequence a short region of COI gene in mitochondrial DNA. The identification of sequenced samples was done by NCBI (98-99%) and BOLD (99%) databases. The sequence data analysis represented 15 variable polymorphic sites and 4 haplotypes. Mean haploty...
Fei Kong1,2, Qingjun Zhu1, Fanrong Xiao1, Jacob Mueti Ngwawa1, Hongxing Zhang2 and Haitao Shi1*
...om natural habitats, the analysis result showed that the species diet was noted to be primarily omnivorous and comprised 23 prey taxa classified into nine groups: plant (4), fish (1), frog (1), earthworm (1), insect (9), mollusk (3), shrimp (2), and other miscellaneous items.

...

Sajjad Khan*, Naila Chand, Abdul Hafeez, Nazir Ahmad 

...lowed during statistical analysis while difference in means was calculated through Tukey’s test. Results indicated that supplementation of GA at 1.5% significantly (P<0.05) improved feed intake, body weight gain, feed conversion ratio (FCR), European Production Efficiency Factor (EPEF) while no effect on livability of broiler chickens. High level (1.5%) of GA significantly (P<0.05) increased the relative weight of heart, liver and gizzard while no ...

Fatma Desouki Mohammed Abdallah

...ource) then, statistical analysis of animal characteristics is of great importance. The objective of this paper was to explain and apply an important statistical method called principle component analysis to extract new carcass trait components of Japanese quail from old variables. The idea of this method is that it forms a new variable (linear combinations of them) by reduction the dimension of the data for a large number o...

Zahid Iqbal*, Farhat Ullah Khan and Jalal-ud-Din

...tan by using time series analysis. Among different appropriate models like (1,0,0), (1,0,1), and (2,0,0) it is based on Normalized Bayesian criterion (NBIC), Akaike information criterion (AIC), Hannan-Quinn and Schwarz Criterion that Autoregressive (AR) model (1,0,0) is best. The Brock, Dechert and Scheinkman (BDS) test is also applied on the residuals of model (1,0,0) for checking the linearity condition before forecasting. The forecasted values for the year ...

Houria Bouazzara1,2,*, Rachid Chaibi 1,2, Farouk Benaceur 1,2,3, Amira Nouioua4 and Laura Bruno 5

...e research duration. CCA analysis indicates that WT, pH, NH4+, NO3-, PO42- and conductivity were responsible for most differences in zooplankton organisms. Finally, we concluded that all sampling area were oligosabrobic indicating the good environmental quality of Wadi M’zi River.

...

Hilarius Yosef Sikone1*, Budi Hartono2, Suyadi2, Bambang Ali Nugroho2 

Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar 

... controls, hematological analysis revealed significant variations (p<0.05) in total leukocyte count, hemoglobin, and mean platelet volume and significant variation (p<0.01) in neutrophils and platelet count. The biochemical analysis revealed a significant rise in serum activity of aspartate aminotransferase (p<0.05), and blood urea nitrogen, blood creatinine, lipase and alkaline phosphatase (p<0.01) in IBD dogs. ...

Fan Da1,3, Zheng-Yong Wen1,2*, Xiao-Dong Wang4 and Yu Luo5

...ertebrates. Phylogenetic analysis suggested the evolutionary process of fish ucp2 was consistent with species evolution, and the pvucp2 shared a close relationship with electric eel ucp2. Quantitative PCRs showed that pvucp2 was extensively expressed in all detected tissues, with the highest expression in liver. Two-week fasting significantly decreased while refeeding dramatically increased the hepatic pvucp2 transcriptions. These findings suggested that fish ...

Moh Zali*, Nur Ihsan, Bambang Ali Nugroho, Kuswati, Umi Wisaptiningsih Suwandi, Riszqina, Suyadi

Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella
...ccess rate of 100%. RFLP analysis of NCOI cutting restriction enzymes shows that there are two bands at 66bp and 40bp. This shows that the normal allele frequency is one and the mutant allele is 0 with a heterozygous genotype frequency of 0% in the population. According to the result, the polymorphism that appears in the CD18 gene by PCR-RFLP method in all samples shows the substitution mutation type G base to C transversion and is not related to the BLAD muta...

Amal Hamad1, Ashraf M. Abu-Seida2*, Faisal A. Torad2, Nahed S. Thabet3, Shabaan M. Gadallah1

...iochemistry. Statistical analysis was carried out by paired samples t-test. Unlike Nalbuphine HCl, Fentanyl citrate and Tramadol HCl, Meloxicam induced better analgesia, no significant changes in total leukocytes, neutrophil, eosinophil, lymphocyte and monocyte counts, serum levels of total protein (TP), albumin, urea, creatinine, creatinine clearance and serum enzymatic activities of alanine transaminase (ALT) and aspartate transaminase (AST) along the whole ...

Shewangizaw Wolde1,2*, Tadele Mirkena1,3, Aberra Melesse1, Tadelle Dessie4, Solomon Abegaz5 

...e. Data were analyzed by analysis of variance. SRSR male chicken was the highest (p<0.05) and LL male chicken was the lowest (p<0.05) in final body weight and feed intake. Feed conversion ratio was best (p<0.05) for LL male chickens. Survival rate of male chicken was not affected (p> 0.05) by genotype. Weights of slaughter, drumstick, thigh, breast, skin, gizzard, neck and total edible carcass were highest (P< 0.05) for SRSR and lowest (P< 0....

Hilaire S.S. Worogo*, Thierry O.T.L. Offoumon, Cham D.A. Alabi, Urbain Tchokponhoué, Yaya Idrissou, Alassan S. Assani, Fade Soule, Christophe Iwaka, Ibrahim Alkoiret Traoré 

Magdy M. Fahmy1, Nisreen E. Mahmoud 1*, Mohamed R. Mousa2, Manal M. Zaki3, Elshaimaa Ismael3, Mai Abuowarda1 

...ions and physicochemical analysis of water revealed deterioration of water quality indicating marked pollution. Parasitological examination of fish samples showed high total prevalence of 68.18% with 10 different ectoparasite species (4 Crustaceans, 4Monogeneans and2 Protozoans) of which the crustaceans recorded the highest prevalence (63.67%). In the present study, Manzala Lake and its corresponding fish farms considered new localities for the detected crusta...

Abdul Wadood Khan1, Sabahat Subhan1*, Asif Ali Abro2 and Riaz Shahid3 

...ulture productivity. The analysis of TFP of the agriculture sector shows a significant variation in the TFP growth, due to climate change patterns, public interference, and macroeconomics strategies. The study recommends a dire need to increase capital stock, skilled labour, and cultivated land to boost and enhance agriculture growth as these are the key factors for the agricultural TFP and economic growth of country’s.
...

Hafeez-ur-Rehman1*, Umar Farooq1, Muhammad Asim Bhutta2, Saghir Ahmad2, Muhammad Akram2, Muhammad Rafiq Shahid2, Hammad Hussnain2, Muhammad Shahid2, Muhammad Mehmood Iqbal2, Ali Raza2 and Muhammad Iqbal2

Ahmed Abdel-Rady1,2, Walaa Mostafa3* 

...tion in this study. Data analysis reported that there was a significant effect for the season and sex on the trichostrongyle infection level; the highest nematode infection level was observed in the cold months (December-April) with a prevalence of 36.44% and lowest prevalence (15.17%) in hot months (May-September) (P<0.05). Females were found to be more prone to infection (31.48%) than males (14.61%) (P<0.05). Young sheep less than 6 months old were sl...

Doaa Khairy1, Mohamed Ali Osman2 and Fatma Abdel Mohsen Mostafa1*

...s mixture. Phytochemical analysis of aqueous neem leaf extract demonstrated the occurrence of a number of flavonoids (21) and phenolic compounds (23) including Hespiridin, Naringin, Rosmarinic acid, Pyrogallol, Salicylic acid and Gallic acid which are implicated in plant preservation and hence induce strength against nematode infection. The results of current study indicated that aqueous leaf extracts of neem, moringa and canola parts in mixture can act as pla...

Yaruq Jabeen1, Nida Ansari1, Haroon Rasheed1, Muhammad Asif Rasheed1,*, Muhammad Awais2, Muhammad Ibrahim1, Sumaira Kanwal1, Aqsa Khalid3, Manzoor Ahmad Zahid4 and Farrukh Jamil1,*

...seven steps. For docking analysis of several ligands, AutoDock Vina is a time-consuming tool. In order to make AutoDock Vina more efficient and smart way, a platform has been developed in this study. It is designated “Let’s Dock”. By using this platform, we can perform a docking analysis of several ligands into a protein with AutoDock Vina by just submitting ligands and protein pdb files. Apart from thermod...

Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr 

...trifugation, and protein analysis was performed by using SDS-polyacrylamide gel electrophoresis. Results showed the presence of the molecular weight of proteins of 71, 52 and 31 kDa in BP and SP; 63, 33, and 19 kDa-proteins in SP, and 28 - 30 kDa-proteins in BP is in association with bull fertility (high semen quality). Also, 48, 18 and 16 kDa-proteins were present in the BP and SP of low fertile bulls. Proteins with 16, 30, 34, 35, 36, 37, 63, 64, 175, and 31...

Rana A. Ali1, Abd-Elraheim A. Elshater2, Heba A. Mohammed3*, Mahmoud Elshazly4 

...ollected for biochemical analysis. Also, the pancreas was dissected for histopathology. Results: biochemically, there was a significant increase in hepato-renal enzymes, cholesterol, glucose, and insulin in the STZ group, while, the other treated groups showed an improvement in such enzymes. Histopathology, STZ induced necrosis, and degenerative changes with vacuolation. Treated groups displayed moderate to mild vacuolation and congestion of the blood vessels...

Tiyyabah Khan1, Hafiz Azhar Ali Khan1*, Muhammad Rizwan Khan1, Muhammad Umer1, Adnan Akhter1 and Waseem Akram2

...espectively. Correlation analysis revealed a highly significant and positive correlation between mortality of T. castaneum and fungal infection inhibition of both fungal species on wheat grains. Mortality of C. ferrugineus had only a highly significant and positive correlation with the infection inhibition of A. parasiticus (p<0.01). In conclusion, the tested insecticides proved effective in controlling C. ferrugineus and T. castaneum with indirect effect o...

Ayu Cusiayuni1, Risan Khaerani Nurfatahillah1, Rakhmad Perkasa Harahap2, Komang Gede Wiryawan3, Dwierra Evvyernie3, Anuraga Jayanegara3* 

...ogenesis by using a meta-analysis approach from published articles. A total of 89 data points from 24 articles were compiled in a database, consisted of various information, i.e., level of lauric acid, microbial population, rumen fermentation characteristics, total gas production, methane production, and digestibility. The data obtained were analyzed by using the mixed-model methodology. Results showed that the addition of lauric acid reduced methane productio...

Zunaira Noreen* and Khawar Sultan

... and principal component analysis (PCA) results showed that out of 29 species using forest as habitat; 13 were “generalists”, 6 species were “rare to classify”, 9 species were “poplar specialist” and only one species was “Eucalyptus opportunist.” Common myna and house crow were the most abundant species. Black kite, house crow, and common myna were found to be making nests on trees only. A clear pattern of habita...

Pan Ziyi1, Ambreen Iqbal1, Gao Zhen1, Liu Juan1, Fang Xibi2, Jiang Ping1* and Zhao Zhihui1

...d through bioinformatics analysis that bta-miR-12059 can bind to 3’UTR of the DHCR24 gene. Our results indicate that single nucleotide polymorphisms might be used as a molecular marker for marker-assisted selection in beef cattle breeding.

...

Taghreed Mohamed Nabil1, Randa Mohamed Hassan1, HebatAllah Hamdy Mahmoud2, Mohamed Gomaa Tawfiek2, Usama Kamal Moawad1* 

...ll as the morphometrical analysis of all parts of the adult turkey hens’ (Meleagris gallopavo) oviduct during the egg production period. For this purpose, twenty four adult turkey hens were used. Gross examination and measurements of the oviduct compartments were obtained, and four specimens for each portion were used for scanning electron microscopic examination. Histologically, pieces of different oviduct portions were subjected to routine histological...

Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*

...s for genetic population analysis. SNP markers that are suitable for non-invasive samples are important to wild population investigations in endangered species. Based on the whole genome sequences of Macaca thibetana, we successfully developed 26 SNP loci that were sensitive to non-invasive samples, then based on which, genetic diversity and population structure of three populations of M. thibetana were estimated. Our results showed the novel SNP loci were pol...

Roshana Mukhtar1, Shaheen Shahzad1*, Sajid Rashid2, Maryam Rozi2, Madiha Rasheed3, Imran Afzal4 and Pakeeza Arzoo Shaiq5

...s in UROS gene. Sequence analysis revealed a pathogenic missense mutation (c.935T>C [p. L237P]) in the exon 10.The sequence was further analysed in-silico to determine the effect of pathogenic mutation on protein structure. In-silico analysis and comparison between UROSL237P and UROSWT 3-dimensional structures revealed remarkable changes in the binding site of Urogen (3-[7, 12, 18-tris (2-carboxyethyl)-3, 8, 13, 17-tetrak...

Abdul Malik Ahmed, Bhargav Bhushan Nath, Ayub Ali, Jiten Sarma, Bipul Phukan, Imtiaz Ahmed* and Jyotirmoyee Das

...ales over males. Monthly analysis data of Gonado Somatic Index (GSI) showed two peaks with one in April and another in August suggesting Gudusia chapra breeds twice a year. The species was recorded to be highly fecund with fecundity ranging from 7,095-48,238. The relationship between fecundity and variables viz total length, body weight and ovary weight were observed to be linear and highly significant (r =0.979).

...

Guo-dong Zhao1,2*, He-ying Qian1,2, Yi-ling Zhang1,2, Gang Li1, 2, Jian Tang1, 2 and An-ying Xu1,2*

...stem. Pathway enrichment analysis was carried out using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Furthermore, some selected differentially expressed genes (DEGs) were verified by qRT-PCR. Our results can facilitate the overall understanding of the mechanism of silkworm’s tolerance to pesticides and provide a new control strategy of Lepidoptera pests.

...

Muhammad Altaf1* Arshad Mahmood Abbasi2, Muhammad Shoaib Amjad3, Sadia Naseer1 and Muhammad Umair4

Junyan Bai*, Jingyun Li, Yu Chen, Zhihao Dong, Youbing Yang and Ying Lei

Thobela Louis Tyasi1*, Lebo Trudy Rashijane1, Kwena Mokoena1, Kagisho Madikadike Molabe1, Madumetja Cyril Mathapo1, Victoria Rankotsan Hlokoe1, Lebelo Joyceline Selala1, Masixole Maswana1 and Lubabalo Bila2
... Pearson correlation and analysis of variance (ANOVA) were used for analysis. Correlation results indicated that egg weight had a statistical significant correlation (P < 0.05) with egg quality traits. Egg weight displayed a positive highly significant correlation with EL (0.82), AW (0.67) and SW (0.62), respectively. The findings suggest that EL, AW and SW might be used in selection to improve EW of Potchefstroom Koekoek...

Ahmed M. Darwish1*, Hassan R. Darwish1, Dalia M. Mabrouk1, Mohamed A. Abdelhafez1, Ahmed M. Abdel-Salam2, Ibrahim E. Mohamed3, Ibrahim M. Farag1 

...en validated by sequence analysis. The results of SSCP-PCR showed a monomorphic pattern for the β-LG gene and a polymorphic pattern for the LEP gene. The sequence analysis showed that the β-LG gene has one genotype without any single nucleotide polymorphism (SNP) while the LEP gene has two genotypes (CC and CT) with one SNP at position 194 C>T. The statistical analysis of milk...

Abida Shehzadi*, Muhammad Shafique and Ahmad Ali Shahid

...STR) haplotype diversity analysis of 143 healthy unrelated male individuals from Jat population of Pakistan. Seventeen Y-STR loci were simultaneously amplified through AmpFISTR®YfilerTM PCR amplification kit. Evaluation of statistical parameters for forensic importance revealed in recognition of 124 unique haplotypes with diversity value of 0.996. Locus DYS385a/b demonstrated highest power of discrimination, polymorphism information content and gene divers...

Kafeel Ahmad*, Muhammad Siqaf, Gul-E-Rana Abid, Ramla Somayya and Umama Qasim

Nadeem Raza1, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Ali Ahmed Sheikh2, Muhammad Usman1*, Quratulain Mujahid3, Muhammad Zahid Iqbal1 and Muhammad Rizwan4
...7% (10/405). Risk factor analysis showed that gender, age and tick infestation are significantly (p < 0.05) associated with occurrence of borreliosis in camels. This study may play an important role in the transmission of borreliosis in understanding of other animal species as well as humans in Pakistan.

...

Azra*, Muhammad Awais, Alam Khan and Muhammad Shoaib

...n attempt of econometric analysis of the investment situation in Pakistan for the period 1990-2017. The variables including domestic credit, national savings, lagged investment (IV), imports, and the real exchange rate were found to be the major factors affecting investment in Pakistan. Among all, the savings coefficient is positive and insignificant because of savings in Pakistan had no healthy pattern. Exchange rate has adversely affected the investment beca...

Thobela Louis Tyasi1*, Lubabalo Bila2, Nkgaugelo Kgasago3, Siza Mthi4 

...ation were used for data analysis. Results showed that the mean live body weight of females and males was 2.11±0.05 and 2.76±0.12 respectively. Correlation analysis revealed that body weight (BW) was positively correlated with heart girth (HG) and rump height (RH) in males, while in females, BW was positively correlated with RH, body length (BL), withers height (WH) and shoulder height (SH). The findings of the...

Ouedraogo Oumar1,2, Tianhoun Denté Fidèle2,4*, Séré Modou3, Kaboré Adama2, Tamboura H. Hamidou2 and Belem Adrien Marie Gaston4

...ions. The results of the analysis of the data collected during these inspections revealed that 1,716 cattle of local breeds, consisting of 875 females and 841 males, were slaughtered and checked by the veterinary inspection officers. At post-mortem inspection, 30 cattle were found to have tuberculosis, giving an overall prevalence of 1.75%. This prevalence showed no significant difference (P˃0.05) between breeds, sex, age and body score of the animals while i...

Muhammad Tahir Latif1*, Muzzammil Hussain1, Ayesha Latif2, Majid Hamid Bajwa1, Iftikhar Ahmad1, Ali Zohaib1, Naeem Faisal1 and Muhammad Hamza3

...bility and profitability analysis of mechanically transplanted rice (MTR) in comparison to conventional manual transplanting (CT). Convenience and snow-ball non probability sampling method was used due to less adoption rate of MTR with a sample size of 240. Among targeted MTR growers 72% had 6-row riding type mechanical transplanters (MT), 18% had 4-row walk after type MT and 10% had 8-row riding type MT. Super Basmati and PK 386 were found more adopted variet...

Judith Kiptoo1*, Daniel Mutisya2, Paul Ndegwa1, Ruth Amata3, Lucy Irungu4 and Rotich Godfrey5

...trans. Factor regression analysis results showed that modest rainfall amounts favoured high density populations of PPNs on citrus roots where soil types of Rackers in Baringo and Luvisols, Ferralsols and Cambisols in Machakos County were dominant. Tylenchulus, Meloidogyne and Helicotylenchus species were most abundant in Kitale, Taita-Taveta, Kilifi and Kwale. The results presented here show how possible population density of PPNs in varied soil class types, r...

Jiang Wu*

...Staph. aureus cells. ATP analysis kit and EnSpire microplate reader were used to detect the intracellular ATP concentration of 4 foodborne pathogens. According to our results of all the tested samples, 38 (28.57%) were found to have Staph. aureus, 27 (20.30%) had Salmonella, 46 (34.59%) had E. coli, and 22 (16.54%) had L. monocytogenes. Staph. aureus had MIC of 0.55±0.18μg/ml and MBC of 1.13±0.11μg/ml; Salmonella had MIC of 0.25±0.13...

Maria Qibtia1, Sehrish Faryal1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Ahmed Mansovuri2, Zeeshan Ahmed2, Muhammad Hamid3 and Ali Raza Awan1,*

... Pakistani patients. Our analysis has evaluated the association between the development of LNP trait and two common variants in intron 13 and 9 (13910-C/T and 22018-G/A) along with the P-value significance level. Therefore in this study, we aimed to access genetic predisposition and clinical manifestations of LNP and LP in 80 subjects (30 subjects with LP and 50 LNP). The presence of T-13910, C-13913, G-13915, G-13907, C-3712, C-13779, A-13937, G-14009, C-1401...

Noora Hassan Hezam Al-Aqmer1*, Zain Aamir2, Muhammad Farooq Hanif3, Soumble Zulfiqar4, Sibgha Zulfiqar1, Mateen Izhar5 and Abdul Rauf Shakoori4*

...ment length polymorphism analysis. After restriction, samples were run on 2% agarose gel followed by visualization under UV light. Data analysis was done using IBM SPSS 24. We found that the distribution of ApaI genotypes was 28 (42.4%), 27 (40.9%), and 11 (16.7%) for the genotypes AA, Aa, and aa in responders and 22 (33.3%), 26 (39.4%), and 18 (27.3%) in non-responders. The allelic distribution was 83 (62.9%) and 49 (37.1%)...

Man-Seok Shin1, Hyun-Ju Cho2 and Eun-Jae Lee3*

...rson’s correlation analysis. Using MaxEnt, breeding area was estimated using the 10 selected variables in Daejeon. The area under the receiver operating characteristic curve (AUC) was 0.950, which was the average value through 10-fold cross-validation to estimate the model reliability. The potential breeding habitat for egrets and herons was estimated to be 106.69 km2 (19.76% of the total area) in Daejeon. Within the estimated potential habitat, 11.82 km...

Aiman Al Mufarji, Abd El-Nasser Ahmed Mohammed* 

...ed and were subjected to analysis through hematology and plasma biochemistry analyzers. The hematological parameters include red and white blood cells (RBCs & WBCs), hematocrit (Ht) and hemoglobin (Hb) values. The plasma biochemistry parameters include plasma glucose, proteins, liver and kidney functions and minerals values. The results indicated that organic M. oleifera leaves contain protein (28.28%), carbohydrate (47.82%), fat (7.57) and fiber (28.35%)....

Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4 

...to be positive. Sequence analysis showed two CPV-2c, six new CPV-2b, and ten new CPV-2a variants. In the CPV-2 studied, several unique and existing mutations have been discovered, indicating the emergence of CPV-2c variation in Iraq.  Genetic differentiation study based on the genetic variation on the VP2 gene has shown that (CPV-2/Sul) strain belongs to new CPV-2b and CPV-2c, however, the (CPV-2/Erbil & CPV-2/krk) strains belong to new CPV-2a. A Phyl...

Luke Chukwudi Ali*, Nnanna Ephraim Ikeh, Bright Chigozie Amaefule, Amarachi Linda Obinna, Ndubuisi Samuel Machebe 

... 3 times using a one-way analysis of variance in a completely randomized design (CRD). Water and feed were made available ad-libitum to the bucks. Diets containing 0, 15, 30 and 45% DCSM were compounded and fed to bucks in T1, T2, T3 and T4, respectively. Prior to semen collection, rabbit bucks were trained three times per week for semen collection with an artificial vagina (AV). At the end of the 10 weeks, semen collection and evaluation were carried out. Thi...

Momotaz Khanom*, Sheikh Mustafizur Rahman, Muhammad Yousuf Ali, Md. Shahin Parvez, Al-Hasan Antu, Md. Nazmul Ahsan 

...< 0.5). Length-weight analysis revealed that the growth of larvae in all treatments showed negative allometric growth (regression slope b ˂3). The highest b value (2.64) was observed in larvae fed with FF. Proximate analysis showed that larvae fed with FF had significantly higher protein content than the other treatments (p<0.05). Water quality parameters remained optimum throughout the experimental period. The feeds ...

Tabassum Ara Khanum, Nasir Mehmood* and Shahina Fayyaz

...tan, several soil sample analysis revealed one new and one known free-living soil nematode species, Neorhabditis andrassyii n. sp., and Poikilolaimus oxycercus which represents a new record of this species from Pakistan. Neorhabditis andrassyii n. sp. is characterized by having male (1109-1222) µm long body, spicules 32-44 µm long, gubernaculum slightly curved, boat shaped with flat distal end eight pairs of bursal papillae were present. Tail (46-6...

Jawad Abdullah Butt1*, Yasmin Nergis1, Ahmad Hussain2 and Mughal Sharif1 

...re fuel. In the coal ash analysis, it can be realized that mostly ash contents are comprised of organic substances and the major compounds are SiO2, Fe2O3, Al2O3, and CaO. In Thermo Gravimetric Analysis (TGA) analysis, Pyrolytic heating rate and temperature were established to have important effects on the pyrolysis of Thar Coal. Kinet...
Ateeq-Ur-Rauf1*,Wisal Khan1, Muhammad Salman Rafi2 and Nauman Khan3
...level was used for trend analysis. Study revealed the fact that except substation Astore all other substations’ precipitation trend is reciprocating temperature trend. Strangely for substation Astore the precipitation trend imitates the temperature trend. It was also found that temperature is changing in three of the four seasons on an annual basis during the study period and there is a variation in the precipitation and Temperature trend patterns among ...

Hamidur Rahman1*, Asghar Ali Kamboh1, Nazar Ali Korejo2, Atta Muhammad Memon3, Rashid Ali Korejo3, Manzoor Ahmed Chandio1 

...respectively. Breed-wise analysis exhibited that 8% and 16% in Lassi breed, 23%, 30% in Kachhai, 10%, 5% in Kharani and 8%, 8% in Brahvi were found positive through RBPT and cELISA respectively. Likewise, area-wise investigation showed that 17.1% and 22.8% in Talli, 13% and 20% in Kurak, 10% and 10% in Khajjak, and 6.6% and 6.6% in Dephal areas were found positive through RBPT and cELISA respectively. Statistical analysis ex...

Abdul Majeed1, Zhenlin Liang2, Lixin Zhu2, Chunli Liu2, Muhsan Ali Kalhoro1,* and Faisal Saeed1

...ctronic length-frequency analysis (ELEFAN) method was used to estimate the VBGF parameters, which were L∞ = 39.9 cm (FL), K = 1.6 yr-1. However, total mortality (Z) was estimated using a length-converted catch curve analysis and found at Z = 5.31 yr-1 with a 95% confidential interval (CI = 4.77-5.84). Natural mortality was estimated at M = 2.16 yr-1 using an average temperature of sea 26○C in Pakistani waters, though...

Jae-Kang Lee, Hyun-Su Hwang, Tae-Kyung Eom, Dong-Ho Lee and Shin-Jae Rhim*

... species for statistical analysis, the striped field mouse (Apodemus agrarius) and the Korean field mouse (A. peninsulae). A. agrarius preferred microhabitat with dense ground vegetation, whereas A. peninsulae preferred understory vegetation. Ground vegetation was reduced as slope gradient increased, but understory vegetation was not affected by slope gradient. The results highlight that the A. agrarius population was influenced indirectly by the negative effe...

Nausheen Irshad1, Maria Akhter1, Tariq Mahmood2*, Faraz Akrim3, Muhammad Rafique Khan1 and Muhammad Sajid Nadeem4

Tariq Mahmood1*, Shakeela Ismail1, Faraz Akrim2, Muhammad Farooq1, Nadeem Munawar1 and Muhammad Raza Khan1

...vasive technique of scat analysis. Results showed grey wolf being distributed at an elevation range of 3921 m to 4282 m above sea level (asl) in the park. Scat analysis showed 7 wild and 6 domestic prey species in its diet, with approximately 47 % contribution from livestock and 53 % from wild prey. Most frequently consumed domestic prey were donkey, yalk, goat and sheep, while among wild prey golden marmot (Marmota caudata)...

Feng Xu, Weijing Fan and Guobin Liu*

...al-time quantitative PCR analysis. The dual luciferase experiment was applied to verify the targeted relationship betweenNEXN-AS1 and miR-410-3p. HUVECs were transfected with NEXN-AS1 overexpression vector, and then treated with ox-LDL for 24 h before detecting the activity, apoptosis rate and oxidative stress level of HUVECs. The low concentrations (25, 50, 100 μmol/L) of rutin had no toxic effect on HUVECs, and the activity of HUVECs was significantly red...
Ade Djulardi1*, Robi Amizar2, Tigor Sanjaya1
...er feed cost (IOFC). The analysis of variance showed that supplementation of expired milk powder in the ration had no significant effect (P>0.05) on ration consumption, daily egg production, egg weight, egg mass, and ration conversion at 20% and 18% protein levels. Based on the study results, it can be concluded that the addition of expired milk powders up to a dose of 0.50% did not affect the performance of quail aged 6-9 weeks. Numerically, higher income ...

Chandni Wajid1, Abdul Hameed Baloch1, Ahmed Nawaz Khosa1*, Hubdar Ali Kaleri2, Nasrullah Bangulzai1, Sarfraz Ali Fazlani1, Haleema Sadia3, Saeeda Kalsoom4, Muhammad Bilawal Arain2, Wassem Ali Vistro2 

...ectively. For PROP1 gene analysis, the blood sample were collected from the selected sheep breeds and processed for DNA extraction. Primers were designed using Primer 3 software and PCR was performed. PCR products were sequenced and analyzed for observing genetic variation in PROP1 gene of these four sheep breeds of Balochistan. The result for PROP1 gene analysis showed 7 variations including 3 frame shift and 4-point mutati...

Mimi Syazwani Jaapar1, Eric Lim Teik Chung1,2*, Nazri Nayan1, Mamat Hamidi Kamalludin1,2, Mookiah Saminathan3, Kalai Vaani Muniandy2, Muhammad Hazziq Mohd Hamdan1, Shokri Jusoh1, Faez Firdaus Abdullah Jesse2,4 

...ere subjected to one-way analysis of variance (ANOVA) and Duncan’s Multiple Range Test to determine the level of significance among treatments. The net gas production and gas production kinetics demonstrated notable changes (P < 0.05) among treatments. Besides, only the acetic acid and total VFA showed significant differences (P < 0.05) for the rumen fermentation characteristics. Generally, the T3 diet consisted of 60% B. decumbens diet demonstrate...

Asim Zubair1*, Iffat Batool2 and Ghulam Akbar Malik3

...gather data. Statistical analysis was done to see the either NGOs were playing their best role in Agricultural Sector or not. To examine the research hypothesis, a chi square test was also used. The findings revealed that NGOs had a favorable impact on agriculture.

...

Noor Muhammad Khan1,2, Tariq Mahmood Khalil1,3*, Rashid Rehan2 and Iftikhar Zeb4

...wnstream. The wastewater analysis showed that HM concentration exceeds the limits of thresholds values. For phytoremediation the T.l was tested in both on-site (in-situ) and laboratory conditions (ex-situ) while A.d was applied only in an ex-situ setup. For T.l, the average uptake of copper (Cu) was 5.40 and 6.46 mg kg-1, similarly for the lead (Pb) average uptake was 4.55 and 4.55mg kg-1, while for nickel (Ni) the average uptake was, 2.80 and 2.76 mg kg-1 in ...

Adimabua Mike Moemeka1, Ufuoma Godstime Sorhue1*, Sylvanus Ikenna Omeje2, Lawrence Bratte2, Raphael Onainor1, Okpara Oghenesuvwe2 

...l + 25% maize. Chemical analysis showed higher nitrogen-free extract (NFE) and lower crude protein content in boiled red sweet potato meal than in the maize. Nutrient digestibility was significantly different (P<0.05) among treatments. The average total weight gain ranged from 13.22kg in T2 to 17.18kg in T1 followed closely by 16.44kg in T3. There were significant differences (P<0.05) among the treatments for all the performance characteristics studied,...

Muhammad Bakhtiar1*, Fayaz Ali Niaz2, Asim Muhammad2, Mamoona Munir3, Wajiha Seerat4, Sadiqullah Khan5, Ghulam Yaseen6, Muhammad Noman Khan7 and Asma Bibi8

... source of sulfur. After analysis of the data the results revealed that Fertilizer applied plots showed best performance in all measured parameters as compared to control plots. In the case of N in 160 kg N ha-1 treated plots, maximum protein content, glucosinolate content, and erucic acid (percent) as well as grain yield was recorded, except oil contents which was higher at 20 kg N ha-1 plots, while on other hand S application, whereas Sulfur applied @ 40 kg ...

Naseem Sharif1,2*, Imran Muhammad Siqqique2, Muhammad Kashif Raza3, Urwa Irshad1, Muhammad Ikhlaq Khan4, Ammara Noreen4 , Muhammad Ahsan Qureshi1, Mohsin Abbas5, Muhammad Maaz Aziz5, Sitwat Riaz5, Komal Aslam5 and Naseem Akhtar6

...ed based on multivariate analysis. Significant diversity was counted for selected morphological and biochemical traits like leaf length (1.8-5.4cm), thorn length (0.6-2.9 cm), fruit weight (1.88-4.72g), fruit length (8.83-19.34mm), fruit width (11.03 -22.74mm), stone weight (0.32-1.09g), TSS (5.9-13.2%) and vitamin C contents (131.2 to 165.56mg/100g). Most positive correlation was noted between leaf length and leaf width (r=0.897) whereas, the correlation betw...

Nawal Abd-elhafez Hassanain1, Raafat Mohamed Shaapan1*, Ahmed Maher1, Ahmed Badwi Yousif2, Gomaa Desoky Emam2 

... blood culture and stool analysis) and brucellosis (Rose Bengal test ‘RBT’ and also standard agglutination test ‘SAT’) a similar time. The results showed that 29 of 504 examined cases (5.75%) were RDTs positive while all examined blood samples were negative for malaria by microscopic blood film examination. Out of the 29 positive RDTs cases 11 were positive for brucellosis (2.18%) and 18 for typhoid fever (3.57%). Our finding concluded ...

Asim Faraz1, Muhammad Yaqoob2, Nasir Ali Tauqir3, Hafiz Muhammad Ishaq1, Ayman Balla Mustafa4, Amir Ismail5, Muhammad Arslan Akbar6, Abdul Waheed1* and Muhammad Shahid Nabeel7

... 18°C for laboratory analysis. Commercially available ELISA kits were used to measure serum progesterone and estradiol levels. Progesterone and estradiol levels were found significantly high (P<0.05) with the value of 3.46±0.25 ng/ml at day 14th and 4.45±0.34 ng/ml at day 21st in G2 (Pregnant animals), while in non-pregnant group serum P4 concentration was recorded to be 1.05±0.29 ng/ml which is considered as basal level of progeste...

Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2** 

...utated. The phylogenetic analysis clearly highlights the aggregation of the sequenced AIV H9 isolates with the Egyptian H9N2 viruses G1 lineage in the group B and the Israeli strains. Although the strains are low pathogenic, the presence of mammalian binding amino acid markers indicated their public health concern. Overall conclusion indicated that AIV (H9N2) still circulates among poultry farms in the Southern Egypt. Furthermore, isolated strains were closely...

Rana Aamir Shehzad1, Ghulam Sarwar1*, Sabir Hussain Shah2, Mukkram Ali Tahir1, Noor-Us-Sabah1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1 and Imran Shehzad1

...re and post-harvest soil analysis was performed. The results showed maximum increase in nitrogen (N) (0.91 ppm) and potassium (K) contents (2.59 ppm) in wheat crop and maximum increase in all plant parameters were recorded in T11 [T2 + all P from PROM (phosphorus rich organic manure)] followed by T13 (T2 + all P from phosphocompost). In case of soil characteristics, application of recommended NPK from chemical fertilizer (T2) recorded the highest pH (8.66) of ...
Bulkaini*, Syamsuhaidi, Yusuf Sutaryono, Djoko Kisworo, Sukirno, Sukarne, Tapaul Rozi
...d regression correlation analysis. The results showed that carcasses with large size categories 77.67%, medium size 17.51% and small size 4.81%, while quality, which includes quality I, was 63.06%, quality II was 32.68%, and quality III was 4.26%. Broiler chicken pure meat products circulating in the traditional markets of Lombok and Sumbawa were respectively 54.21% and 57.83%. Conclusion: Carcass characteristics and pure meat production of broiler chickens ci...
Yasmin M.M. Mahmoud
...d on control (T0). Blood analysis and hematological characteristics were also estimated. It is recommended to replace berseem hay with dried broccoli by-products up to 20 % in the diet of lactating goats regarding their digestibility, productive performance, blood parameters and hematological profile.
 
Keywords | Goats, Broccoli wastes, Productive performance, Digestibility
...

Sri Melia1*, Indri Juliyarsi1, Yulianti Fitri Kurnia1, Evy Rossi2, Hurriya Alzahra3

...identification of LAB by analysis of the base sequence of the 16S RNA gene. The whey for this study was collected in Lasi Farm in Agam Regency, West Sumatra, Indonesia. The data was subsequently subJected to descriptive analysis. The bacteria obtained were rod-shaped that were catalase negative and homofermentative. Lactic acid bacteria were found to have antibacterial activity against pathogenic bacteria such as E. coli, Ps...

Oluwaseun Adetarami1*, Oluwatoyin Olagunju2, Babatunde Adebayo Oyebamiji1, Adegboyega Abel Odeyemi3 and Sina Basil Johnson4

...stionnaire. For the data analysis, statistical tools such as budgeting technique and logitistic regression were used. Results of the budgeting technique showed that each farmer of the FBS approach had a mean profit of ₦ 81,134.65 with a cost benefit ratio of 1.09. The results of logistic regression revealed that the age of cocoa farmer, level of education, extension contact, farm size and cocoa annual income are major determinants influencing the utilization...

Saima Mehar1 and Salma Javed2*

...formed for phytochemical analysis and to assess the nematicidal and nematostatic potential of crude extract of Withania coagulans. It is known for its withanolide class of chemical compounds. The biological assessment of two primary fractions of W. coagulans crude extract, chloroform and butanol, is the focus of the current study. Crude extracts were tested against Meloidogyne incognitain vitro conditions. The second stage juveniles (J2) were exposed at 24, 48...

Amal Ramadan Fawy1, Hussein Yousef Ahmed2, El-Sayed S.E. Shabana3, Mohamed Abdelfattah Maky4*

...y. Proximate and quality analysis were conducted. Group D of Egyptian beef burger had a greater protein than other groups as well as being the best in terms of quality. Furthermore, six beef burger formulations with different amounts of beef fat and vegetable oils were prepared and stored at 4 ± 1°C for 21 days. Analysis of samples showed that fat content was significantly lowered in F1 and F2 (beef fat partially ...
Amreen Zahra1*, Mushtaq A. Saleem2, Hasnain Javed3, Muhammad Azmat Ullah Khan4 and Abdul Rauf Shakoori4
...ecular and computational analysis of the major genetic constituents, Gag-Pol region of HIV-1 for the very first time in Punjab-Pakistan including three regions of outbreak i.e. Kot Imrana Sargodha, Faisalabad and Gujrat. Drug resistance mutations such as T12A, I13A, K14R, I15V, K20I, T31S, E35D, M36I, N37D, R41K, K43X, R57K, Q61H, H69K, K70R and L89M were observed across several sites across the whole length of PR region among the isolates. A significant acces...

Xing Lu1, Mahmoud A.O. Dawood2, Fan Wu1, Hua Wen1, Wei Liu1, Juan Tian1, Ming Jiang1, Li-Juan Yu1, Xiang Li3, Ning Xu3 and Hong-Wei Liang1*

...erum biochemical indices analysis revealed that dietary L-carnitine at 400 mg kg-1 had markedly reduced triglyceride (TG) concentration (P < 0.05). The elevated expression of hepatic igf1 gene at the transcriptional level was positively correlated with dietary L-carnitine levels on the growth performance of Chinese soft-shelled turtle. Furthermore, dietary L-carnitine supplementation significantly up-regulated the mRNA levels of superoxide dismutase 1 (sod1...
Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1
...us, Cattle, Phylogenetic analysis, 5`UTR
...

A. El-Shemy1*, Hoda M. Mekky2, M. A. Bosila2, A. M. Allam1, Kh. M. Elbayoumi2, M. M. Amer3 

...tide sequences alignment analysis of the 3D gene exhibited 92.5%–98.6% homology between our sequenced isolates. The three examined isolates had 77.4%–80.2% nucleotide similarities with the most frequently used DHAV-1 vaccine in Egypt. However, the phylogenetic relationship using the partial 3D protein revealed that the isolated Egyptian strains in this study were distant from the vaccine strain used in Egypt. In conclusion, three isolates of DHAV-1...
Baila Ahmad1, Muhammad Ammar Khan1*, Zulfiqar Ahmad1, Rana Muhammad Bilal2 and Asghar Ali Kamboh3
...Finally, the correlation analysis showed that industrial, as well as consumer acceptability were influenced by dietary Zn through moderating roles of pH and WHC.

...

Limei Yuan1, Kong Yang1* and Dechun Jiang2*

...ection and biogeographic analysis of these species.

...

Madiha Khalil1, Amjad Farooq1, Asim Faraz2*, Abdul Waheed2, Nasir Ali Tauqir3, Riaz Hussain Mirza2 and Hafiz Muhammad Ishaq2

...subjected to statistical analysis using fixed effect models. Independent variables were year of birth, season of birth, year of calving, season of calving and parity. Age of animal (linear and quadratic effects) and lactation period were fitted as co-variables. Dependent variables were lactation performance traits (lactation milk yield, dry period, etc.). The results of all traits showed that the year of calving had a highly significant (p<0.01) effect on l...

Khalid Javed Iqbal1, Irfan Baboo2*, Arshad Javid3, Noor Khan4, Hamid Majeed5, Azra Anwer1, Zahid Farooq2, Muhammad Altaf6 and Moazam Jalees7

...eriod of 96 hours by the analysis of their haematological parameters. Fish were segregated into three groups (10fish/treatment). The iron oxide nano-particles at 0.25g/l doze were administered orally to treatment T1 and intravenously from caudal vein of fish to treatment T2. After 96h of exposure period, the blood was collected from fishes by the caudal vein puncture method into tubes containing anticoagulants EDTA and transferred immediately to the laboratory...

Abdul Ghaffar*, Niaz Hussain, Muhammad Nadeem, Khalid Hussain, Muhammad Aslam, Mudassar Khaliq, Muhammad Irshad, Zubeda Parveen and Muhammad Younas

...d to principal component analysis (PCA), correlation, and path coefficient analysis. Analysis of variants revealed good variation among the accessions for the data recorded traits. Principal coefficient analysis differentiates all the traits into six PCs. Two components expressed more than one Eigenvalue which collectively contributed 80.12% towards the ...

Khalid Khan1*, Ahmed Farhan Saeed2, Sanam Wagma Khattak3 and Saima Liaqat4

...ient test and regression analysis are carried out. The study accentuated various factors which are responsible for the adaptation of poultry technologies and their standard practices. The study found that the lack of education, training, availability of credit and information, managerial skills, low income, and market accessibility are the key impediments to the adoption of advanced technologies and standard practices in the poultry business in the district. N...

Dede Kardaya*, Dewi Wahyuni, Elis Dihansih 

...ata were subjected to an analysis of variance and a Duncan’s multiple range test. Results revealed that meat cooking loss and fat content were significantly lowered (P<0.05) in ducks treated with treatment rations. No significant differences were found in other physical and chemical quality parameters. It was concluded that supplementation of 2, 4, and 6% AGLM reduced cooking loss and fat content of spent layer duck meat.

Keywords | Antioxidan...

Mostafa El-Sebelgy1*, Hanafy Madbouly2, Sabry Tamam2, Nagwa Ata1, Kawther Zaher1

 

...-free) shotgun proteomic analysis. The resultant peptides were used along with RefSeq proteins and primers for multiple sequence alignment. Multiple extrinsic proteins [NS1, NS3 and VP3 of African Horse Sickness Virus (AHSV) and NS3 of Equine Encephalosis Virus (EEV)] were detected and at the same time, integration events were examined in this study. This research work entails unpredicted integration of foreign non-BTV viral proteins (NS1, NS3 and VP3 of AHSV ...

Muhammad Ajmal Khan1, Muhammad Yahya2*, Ali Hazrat2, Javed Khan3, Saeed Jan4, Tabinda Nowsheen2 and Inam Ullah5

...ro;L). The phytochemical analysis of methanolic leaves extract of T. camphoratum showed 2.80% alkaloids, 4.90% flavonoids and 1.80% terpenoids content. The antibacterial activity of the methanolic extract showed high effectiveness against Gram-positive bacteria (MRSA) with 23 mm zone of inhibition (ZOI), whereas against Gram- negative bacteria, S. typhi, E. coli and P. aeruginosa, with ZOI observed was 22, 18 and 20 mm, respectively. Antioxidant activity evalu...
Tahreer Mohammed Al-Thuwaini*, Ali Basim Abd Al-Hadi
...Awassi ewes. Association analysis of lamb sex with body measurements of pregnant ewes, including singleton and twin, showed that male pregnant Awassi ewes had higher body dimensions than the female pregnant Awassi ewes. There was the highest correlation (P ≤ 0.05) between lamb sex with live body weight and body dimensions of ewes with single and twin pregnancies. In conclusion, twin and single pregnancy ewes with heavier live body weights and higher body me...
Ali Basim Abd Al-Hadi*, Tahreer Mohammed Al-Thuwaini, Mohammed Baqur Sahib Al-Shuhaib
..., CG, and GG. Sequencing analysis revealed a novel mutation c. 25760691 C>G in the CG genotype. Association analysis revealed significant differences (P ≤ 0.05) between CC and other genotypes in reproductive hormone levels. Estrogen and progesterone were significantly higher (P≤0.05) and follicle-stimulating hormone (FSH) levels were lower in the CC genotype (85.10 ± 3.11) (pg/ml), (8.82 ± 0.52) (ng/ml...

Lyudmila Yakovlevna Rodionova*, Irina Valeryevna Sobol, Lyudmila Vladimirovna Donchenko, Artem Vasilevich Stepovoy  and Andrey Georgievich Koshchaev

...of physical and chemical analysis of plant materials were used. The biochemical parameters of pumpkins were studied: the content of dry substances, sugars, titratable acidity, the content of ascorbic acid and carotene. Data on the content and fractional composition of pectin substances in different parts of pumpkins are presented. The kinetics of changes in the content of pectin substances in pumpkins after harvesting and storage for 90 days are shown. To asse...

Muhammad Jamil1*, Kamran Javed1 and Imran Akhtar2

...udy. Principal component analysis, along with agglomerative hierarchical clustering tools were employed. Twenty-four recently bred upland cotton strains bearing diversified origin were configured in triplicate following Randomized Complete Block Design (RCBD). The data for novel attributes were recorded. Analysis of variance results indicated that a significant level of variation was existing among the strains for disease in...

Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*

...DS-PAGE and Western blot analysis. The purified protein was then used as a coating antigen to establish an indirect ELISA method for detecting the T. gondii antibody in pet cats, whose reaction conditions were optimized. The SAG2 gene was successfully cloned into a pET-21a (+) prokaryotic expression vector, and the recombinant plasmid pET-21a-SAG2 was obtained. The size of the recombinant protein was approximately 19 kDa, and it was expressed mainly in the for...

Muhammad Furqan and Zulfiqar Ali

...hmir, Pakistan. The diet analysis was done through crop contents of kalij pheasants, threats were assessed through field surveys, communities meeting, and data from 250 questionnaires during April 2020 and March 2021. Based on analysis of threats data, conservation management strategies are recommended. The diet analysis showed that mostly the kalij consumes plant matter as the major diet....

Qian Chong1, Muhammad Mohsin2*, Zhu Ting1, Chen Qiqi1 and Ana Mehak3

...f computer-based fishery analysis software, i.e., catch and effort data analysis (CEDA) and a stock production model incorporating covariates (ASPIC). In the principle initial portion (IP = 0.9), CEDA output showed higher values of maximum sustainable yield (MSY) (480000 – 614000 t) as compared to ASPIC (468000 – 583000 t). Similarly, R2 values followed the pattern of estimation with MSY. Research findings signpo...
Fei Wang1, Xiaofen Hu1, Feng Wen2, Xiaoen Tang3, Shanshan Yang1, Shengwei Zhong1, Zuohong Zhou1, Xu Yuan1 and Yong Li1,*
...ariety of bioinformatics analysis was performed to evaluate its structural characteristics. CDS of vasoactive intestinal peptide, encoding 132 amino acids, is 399 bp long in Wahui pigeon. Vasoactive intestinal peptide probably belongs to a hydrophilic peptide without any signal peptide and transmembrane structure, and mainly plays its biological function due to occupying 89.6% in cytoplasm. Furthermore, it consists of one N-glycosylation site and 17 phosphoryl...

Tasya Nur Karina1, Osfar Sjofjan2, Tri Eko Susilorini2, Muhammad Halim Natsir2*

... five replications. Data analysis from this study Analysis of Variance (ANOVA) and Duncan’s Multiple Range Test (DMRT). The results show that the treatments had no significant effect (p >0.05) on production performance and egg physical quality. To sum up, using date palm (Phoenix dactylifera L.) has positive effect on the growth performance and egg quality of ISA BROWN laying hen because can be alternative feed ingr...

Hairul Islam M. Ibrahim1,2, Abdalla A. Sayed1,3, Abdel Naser A. Ahmed4,5 and Emad A. Ahmed*1,6

...aluated by histochemical analysis. T-cell population and cytokines levels in myelin oligodendrocyte glyco-protein (MOG) challenged PCB treated cells were estimated using flow cytometry and ELISA kits. Apoptotic and neuronal markers were evaluated using quantitative real time PCR and protein blots. Results showed that PCB inhibited the infiltration of inflammatory cells and improved the myelin protective proteins. It also positively regulated the antioxidants a...

Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2

...olates with phylogenetic analysis reveals the full identity between the Egyptian isolates from the outbreaks and other EHV-4 strains available in databases proving wide distance with other EHV-8 and EHV-1. The study recommends rt-PCR as a screening test for the tentative diagnosis of EHV-4 in epidemiological studies and n-PCR as a sensitive differential test for the virus detection. The virological studies and molecular assays confirm the involvement of EHV-4 ...

Muhammad Akram1*, Muhammad Imtiaz Shafiq2, Amber Malik3, Farmanullah Khan4, Munir Ahmad Bhinder5 and Muhammad Sajjad6

...ng informed consent. The analysis of the three allelic variants GSTM1, GSTT1, and GSTP1 was carried out through PCR-based amplification. Amplification of GSTM1 and GSTT1 was performed using the specific primers designed by Primer-3 software. GSTT1 and GSTMI genotypes were determined by comparing the sizes of amplified PCR product of genotypes with β Globulin gene, used as internal standard and 100-bp DNA ladder. GSTP1 genotype was determined using the PCR...

Xinran Wang1,2 and Lizhi Zhou1,2*

... was compared by one-way analysis of variance (low disturbance: F = 1.854, P < 0.001; moderate disturbance: F = 1.854, P < 0.001; high disturbance: F = 1.637). The relationship between disturbance intensity and vigilance mode was determined by analyzing the influence of disturbance intensity on the vigilance mode of the crane group. Anthropogenic disturbance intensity had a significant influence on vigilance mode of the wintering cranes. However, there w...

Agus Mulyono

...hrough color and texture analysis of Java sparrow beak image with classification method using ANFIS and determine the level of accuracy. In this study there are 3 stages, namely preprocessing, learning stages and testing stages. The results of the Java sparrow sex detection accuracy through texture and color analysis of beak images using the ANFIS method are 90%. This shows that texture and color can be used to detect the se...
Samreen Fatima* and Muddasir-Uddin
... 2019 are considered for analysis. Various forms of GARCH-M models are applied by inserting conditional standard deviation, conditional variance, or conditional log variance in the conditional mean equation. Moreover, enhancement in the forecasting performance by combining the GARCH-M and ANNs (hybrid model), developed GARCH-M-ANNs model could be seen clearly. As per Akaike Information Criterion (AIC) and Schwarz Bayesian Information Criterion (SBIC), the stud...

Mehroze Amin1, Afifa Yaqub1, Qindeel Fatima1, Rabail Hassan Toor1,3 and Abdul Rauf Shakoori1,2*

Jiacheng Du and Yuping Cao*

... outcomes, and ROC curve analysis of serum AMH levels in the predictive value of IVF-ET clinical pregnancy in PCOS patients. Subjects in Group A showed significantly lower FSH, LH, and T levels (P<0.05) than Group B. Compared to patients in Group B, patients in Group A also showed significantly higher rate in implantation rate, high-quality embryo rate, biochemical pregnancy rate as well as clinical pregnancy rate (P<0.05), while the incidence of early m...

Jie Zhang, Na Li, Tingting Guo, Dandan Hu, Xiaofeng XU and Lili Zhang*

...16S rDNA high-throughput analysis. The rumen fluid was collected from 9-month-old Tan sheep that were either house-fed or grazed. The results showed that the diversity of rumen flora in the grazing group was significantly higher than that in the house-raised group (P < 0.01). In the grazing group, the abundance of 96 genera was significantly higher than that in the house-raised group (P < 0.05), whereas in the house-raised group, the abundance of only 24...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
...s provided by expression analysis of the G9a gene and genes associated with cell proliferation, tumor suppression, and apoptosis. The gene expression of fibroblast growth factor 1 (FGF1), enolase 2 (ENO2), cyclin D1 (CCND1), catalytic subunit of AMP-activated protein kinase (AMPKα2), RNA polymerase II transcription elongation factor (ELL2), and pro-apoptotic Bcl-2 homology 3-only protein (BIM) in MCF-7 breast cancer and normal human embryonic kidney HEK-...

Gawhara Ahmed-Abdelmonem1*, Zeinab Aboezz2, Ahmed Habashi1, Saad Sharawi2 

...gene. Through phylogenic analysis of three FMDV serotype A isolates, it has been deduced that two isolates were clustered to Asian Iran-05 topotype with close similarities (99.42-99.57%) to the local vaccine strain, while the other one belonged to African topotype genotype IV (GIV) and was genetically different from the local vaccine strain with 85.65% identity. These findings support the suggestion of adding the African topotype GIV as a supplementary vaccine...

Xiangli Dong1,2, Shilin Mikhail Borisovich2, Jiji Li1,*, Jianyu He1, Zeqin Fu1, Yingying Ye1, Julia N. Lukina3, Olga V. Apalikova4 and Jianshe Zhang1

... real-time PCR (qRT-PCR) analysis indicated that MRC1 and MRC2 mRNAs were expressed in eight different large yellow croaker tissues, and that their expression was up-regulated by Vibrio anguillarum challenge. Here, we performed membrane protein analysis and epitope analysis to select MRC1 and MRC2 protein fragments suitable for antibody production. We then PCR amplified L.c-MRC1 and L.c-MR...

Muhammad Hassan Naseem1, Muhammad Mansha1*, Muhammad Triq1 and Peter C. Thomson2

Zaidi Zona, Zulfiqar Ali*, Rida Ahmad and Irfan Zainab

...ata, principal component analysis (PCA) was done by using XLSTAT and excel 2019 to study the factors affecting the water quality. The eigenvalues obtained after PCA from zone 1 to zone 5 were 5.40, 2.40, 1.90, 0.28 and <0.28, respectively. The higher eigenvalue of zone 1 suggests large dispersion of data in this region. Furthermore, the findings in the study indicate that warm water, higher conductivity, DO, high nitrate concentration, and high salinity man...

Nguyen Hai Quan, Nguyen Huu Van*, Nguyen Thanh Thuy, Vo Thi Minh Tam, Le Duc Thao, Le Duc Ngoan 

...ay 0 and 21 for chemical analysis. Results indicated that after 21 days of ensiling, the pH value of silage in 8 treatments were lower than 4.5. There was no effect of additives and their level inclusions on ensiled sunflowers nutritive values. Change in organic matter was found with 0.7-2.3% unit in all treatments. In general, whole Aguara 6 sunflower can be ensiled and has a potential for ruminants.

Keywords | Sunflower, Biomass yield, Nutritive valu...

Mutassim Abdelrahman1, Ibrahim Alhidary1, Majdi Bahaddi1,2, Mohsen Alobre1,3, Riyadh Aljumaah1 and Rifat Ullah Khan4, *

...re collected for mineral analysis by inductively coupled plasma optical emission spectrometry. Results indicate that there is no significant difference (P >0.05) between the concentration of all the elements in all lambs’ tissues, except for the concentration of aluminum in kidney and rumen fluid, as well as Mn in rumen fluid. Moreover, Mn concentration in rumen fluid was significantly (P < 0.05) decrease with zeolite supplementation, with signific...

Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5

...ontrol. In genetic model analysis, we found strong association (OR, 0.4 (0.181 to 0.94), p = 0.03) of GG genotype with male with EPTB as compared to female in gender stratified dominant model. Furthermore, in context of different ethnic groups of Pakistani subpopulation, we found Punjabi speaking’s to be significantly at higher risk (OR, 2.16 (95%CI, 1.0910 to 4.2901), p = 0.02) of developing TB as compared to other ethnic categories. Strong association ...

Qi Zhuo, Yuanchun Yao, Meisongzhu Yang*, Jinhua Chen and Miao Tian

...this study. Western blot analysis of miR-216b-5p and HDAC8 protein expression in tissue samples; reverse transcription quantitative PCR (RT-qPCR) real-time analysis of miR-216b-5p, HDAC8 mRNA expression in cell lines; MTT detection of cell proliferation; wound healing test and transwell assay were used to evaluate the ability of breast cancer cells to metastasize and invade; colony formation test was conducted to detect the ...

Zhang Yan1,2 and Muhammad Mohsin3*

...A (catch and effort data analysis). For the principal input (IP) value of 0.9, the former software suggested a maximum sustainable yield (MSY) of around 65,000 tons (t), whereas the latter one calculated MSY as 71,000 t. In ASPIC, coefficient of variation (CV) and R2 for Fox Model (F-M) and Logistic Model (L-M) remained at 0.113, 0.982 and 0.055, 0.895, respectively. Meanwhile, in CEDA, MSY outputs remained 72,082 t and 80,723 t (on average) for F-M and Schaef...

Victoria Rankotsane Hlokoe, Thobela Louis Tyasi* 

...t 

...

Febriza Dwiranti1, Nur Fadhilah2, Ursula Paulawati Maker2, Priyo Sambodo3* 

...rentiation by laboratory analysis. Descriptive morphological data and quantitative data were analyzed using ANOVA testing. The adult cuscus appeared to have more spots (the male spotted pattern was larger than that of the female) than the juvenile cuscus, where the body pattern had not formed spots (spread). Based on such an obvious spot pattern, the sexes of S. papuensis could be distinguished by males having larger spots than females. The S. papuensis juveni...

Khurram Sheraz* and Taj Ali Khan

...ext-align: justify;">The analysis of the meteorological indices provides valuable information about the evolution of climate during past and may predict its reflection in the future. Analysis of trends in meteorological variables is challenging as they are of non-stationary nature and may include both stochastic and noise components. The aim of this research is to integrate the signal processing technique Discrete Wavelet Tr...

Muhammad Jamil1*, Muhammad Hussnain Babar2, Aamir Hussain3, Rana Abdul Hameed Khan3, Hammad Hussnain4 and Saghir Ahmad4

...ative interaction (AMMI) analysis illustrated that additive effects of genotypes, environments and multiplicative effects (GEI) were significant at (p<0.01). Test sites accounted for the biggest share (74.1%) of the variability, followed by GEI (17.2%) and strains (8.7%), respectively. The first four interaction principal components (IPC) were significant at (p<0.01) wherein the first two IPCs captured (68.4%) of the GEI cumulatively. Strain ST06 (FH-492...

Auzair Javaid Butt, Irfan Ullah*, Shahid Ali, Khurram Nawaz Saddozai and Jahangir Khan

...ler → Consumer. The analysis shows that marketing-margins of potato growers, wholesalers and retailers is estimated as 31.8 percent, 24.1 percent, and 42 percent respectively. Similarly, the share of producer in consumer rupee is obtained as 31.3 percent, respectively. Marketing margin and cost indicated that by reducing the huge margin of retailers the share of growers and wholesalers can be raised in the prevailing marketing channels. The research findi...

Desy Cahya Widianingrum1*, Himmatul Khasanah1, Melinda Erdya Krismaputri1, Susan Barbara Patricia Sembiring Meliala2 

Djoko Kisworo*, Martina Safitri, BRD Wulandani, Bulkaini, Syamsuhaidi 

...nalyzed according to the analysis of variance followed by the Least Significant Difference test (LSD). The parameters measured were the egg’s weight, air cavity, pH, and Haugh Unit of chicken eggs that were stored at room temperature for six weeks. The results showed that immersion in SSF and storage decreased (P<0.5) the HU and egg’s weight. While the air cavity and pH increased significantly (P<0.05). The treatment of 0% SSF, 10% SSF and 20...

Nuraini Nuraini*, Yuliaty Shafan Nur, Ade Djulardi, Robi Amizar, Yesi Chwenta Sari

...at. The Phase 2 variance analysis results showed that the use of Tmc in the diet had no significant effect (P> 0.05) on feed consumption, egg production, egg weight, egg mass, and feed conversion. The study concluded at stage 1 that 100% tofu dregs medium was the best treatment to improve the nutrient content of the Tenebrio molitor caterpillar. Stage 2 concluded that the Tenebrio molitor caterpillar could be used up to 12% in laying quail rations (substitu...

Sri Suharyati, Siswanto, Madi Hartono, Kusuma Adhianto* 

...perm were analyzed using analysis of variance ANOVA. The results showed that the administration of vitamin E and L-carnitine exerted a very significant effect on motility (P<0.01), the concentration, and the percentage of live sperm (P<0.05) but did not affect the sperm volume and abnormalities (P>0.05). In conclusion, the combination of vitamin E and L-carnitine gave the best results on the sperm quality of the Jawarandu Goat.

Keywords | Jawa...

Ali Wahdan1*, Mahmoud Ezzat1, Fatma Youssef2, Manal Munier2, Elsayyad M. Ahmed3, Marwa Abo Hashem1  

...nce factors with genetic analysis of recovered isolates. A total of 350 random samples were collected from animals and hospital acquired infected patients. The recovered isolates were identified biochemically by vitek2 compact, confirmed by PCR and tested for sensitivity against varieties of antimicrobials. Three multi-drug resistant isolates carried ica from human abscess (A9), sputum of dog (A12), and cow mastitic milk (A13) were sequenced with other genom...

Gudeta Nepir Gurmu, Tade Bitima Mulisa, Alemu Lenco Gemechu, Kegna Gadisa Amena and Gemechu Nedi Terfa*

Caiyun Ma1, Hebao Wen1, Lu Li2, Gaofeng Liu1, Chunjing Wang1, Yu Guo1, Shiji Fang1, Hongyu Wang1* and Changqing Liu1*

...wth curves and karyotype analysis, respectively. The results showed that the CSPCs of Tibetan mastiff displayed self-renewal ability, proliferative potential, and hereditary stability. Moreover, the CSPCs of Tibetan mastiff also positively expressed recognized surface antigens of human CSPCs. Additionally, CSPCs exhibited differentiation multipotency, including osteogenic, adipogenic and chondrogenic potential in vitro. These findings may propell CSPCs applica...

Madumetja Cyril Mathapo and Thobela Louis Tyasi*

...ssion were used for data analysis. Phenotypic correlation outcomes indicated that SC had a positive statistically significant (P<0.05) correlation with BW (r = 0.425) and non-significant correlation with BL (r = 0.108), HG (r = 0.082), RH (r = 0.038), WH (r = 0.097) and SH (r = 0.280), while TL had non-significant correlation with BW (r = 0.009), BL (r = -0.351), HG (r = -0.147), RH (r = -0.108), WH (r = -0.086) and SH (r = 0.052). Regression

Fatin Khalil*, Harinath Yapati, Zainab Al Blallam, Ronia Jose 

... biochemical, hematology analysis and production performance of Naeemi sheep were affected by the seasons and stage of production and growth.

Keywords | Naeemi sheep, Seasonal effect, Intensive management, Reproductive performance, Baseline data. 

...

Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3

...firmed with Western Blot analysis. The study demonstrates the expression and purification of HDAg recombinant protein of HDV local isolate in the bacterial expression system. The purified protein has potential to be useful as an antigen in Diagnostic ELISA and as a vaccine candidate.

...

Lei Xu1, Qin Yang2 and XiaoYan Zhang3,*

...s of Pearson correlation analysis revealed that the level of sclerosin in serum was in positive correlation with age, serum phosphorus, iPTH, FGF-23, AACS, SPB and Creatinine (CREA) (P < 0.05), but in a negative correlation with the levels of 25(OH)D and calcium in serum and eGFR (P < 0.05). Results of logistic regression analysis revealed that AACS, eGFR, FGF-23, iPTH, serum phosphorus and 25(OH)D were the independent...

Sajjad Ali1*, Atta ur Rahman1 and Sher Ali2

...satellite imageries. The analysis showed a persistent increase in built-up areas from 1990 to 2020 as the built-up area increased by 57090 hectares. However, the barren land indicates a decreasing pattern of growth, decreasing by 102452 hectares in Peshawar valley. The analysis disclosed a consistent increase in the Production of crops. On the other hand, the per capita share of inhabitants in crop production showed a decrea...

Muhammad Halim Natsir*, Osfar Sjofjan, Yuli Frita Nuningtyas, Danung Nur Adli, Lizar Basnelda, Dimas Frisky Apriyanto, Wahyu Tri Utami 

...ffect (p < 0.05), the analysis continued with least significant differences (LSD). The result showed significantly difference (p < 0.01) on villus height and significant difference (p < 0.05) on microbial population. In contrast, the result showed no significantly difference (p > 0.05). In conclusion, combinations of nano-fountain clerodendrum and avocado seed flour help to increasing villus height and adverse microbial on Lohmann broiler chickens....

Olufemi Bolarin, Sijuade Adebukola Adebayo and Sola Emmanuel Komolafe*

...The result of regression analysis showed that coefficient value of farm size (p=0.017) and membership of cooperatives (p=0.013) positively enhanced resilience building used to mitigate the effects of climate change. The study further averred that inadequate finance, traditional belief, and inadequate access to new technologies were serious constraints encountered by yam farmers. The study recommends that government in collaboration with development agencies sh...

Zulqarnain Haider1*, Muhammad Akhter2, Syed Sultan Ali1, Tahir Latif1, Rana Ahsan Raza Khan1, Awais Riaz1, Tahira Bibi1, Muhammad Ijaz3, Qasim Raza1, Mohsin Ali Raza4, Samina Sarfaraz1, Muhammad Iqbal1 and Muhammad Rafiq1

...nments. Test-environment analysis indicated that genotypes PK10324-1-1 and PK9444-8-1-2 were best suited for 2017 season, genotypes PK10967-30-1, Chenab Basmati and PK 10355-13-2-1 were best suited for 2018, genotypes PK9966-10-1 and PK 10434-6-2-1 were best suited for 2016 season, whereas genotype PK10029-13-2-1 performed equally well in 2017 and 2018. Principal Component Analysis (PCA) also displayed stability in performan...

Onwusiribe Ndubuisi Chigozirim*, Okpokiri Chibuzo Ikechukwu, Agu-Aguiyi Fortune Nneka and Oteh Ogbonnaya Ukeh

...uired data. For the data analysis, descriptive statistics and correlation analysis were used to analyze the data. The findings revealed that the socio-economic status of rural farmers, especially income and education are significant factors influencing their access to institutional support and adaptation to climate change. This study suggests that the government take a multi-stakeholder approach to addressing climate change,...

Hafiz Qaisar Yasin1*, Dora Marinova2 and Muhammad Naveed Tahir3

Muhammad Jamil1*, Ghulam Sarwar2, Imran Akhtar3, Ghayour Ahmad4 and Saeed Ahmad4

...ative interaction (AMMI) analysis procedure was followed for the data analysis. The analysis of variance revealed that strains, environments and genotype into environments interaction (GEI) results were significant at (p≤0.01). Further, the GEI sum of squares (SS) was comprised of (20.5%) out of total variability. Seven interaction Principal Component Axis (IPCA) were found significant ...

Shandana* and Ayesha Khan

...duction. Krushkal Wallis analysis showed that there was highly significant difference in respondents’ perception about the effect of mobile, telephone, television and radio on production. The study suggested that extension agents are required to create awareness of the use of ICTs among the farming communities. Department of agriculture extension needs to establish an ICT based program so that innovative agricultural information might be communicated to ...

Hafiz Kashif Ali1, Iftikhar Ahmad1, Mujahid Ali2*, Zahoor Hussain3, Muhammad Ather Nadeem4, Malik Abdul Rehman5, Barkat Ali6, Muhammad Iftikhar7

Muhammad Ikram Ullah

...omozygosity linkage. The analysis for genotype assessment was carried out, and three homozygous regions were identified. Whole exome sequencing was carried out to identify the mutation in putative gene/s. The data was arranged, and MAN2B1 was selected for the DNA sequencing. Protein homology was analyzed by the pymol tool to predict the effect on the mutant protein. A novel missense mutation c. 2710A>T; p.904Tyr>Ser was detected, and co-segregation

Juweria Abid1, Shakeel Ahmad2, Humaira Wasila3, Tooba Asif4, Muhammad Farooq5* and Attaullah Jan6

... for further biochemical analysis. Samples were then analyzed for minerals content, total phenolic compounds, total flavonoids and antioxidant activity. Physicochemical characteristics, proximate composition and energy density were also estimated. Statistical analysis showed significant differences (p <0.05). Fat content (6.73±0.06%) and total solid (15.21±0.01%) were found highest in Buffalo milk. Mineral <...

Shakeel Ahmad1*, Humaira Wasila2, Juweria Abid3, Nazir Muhammad4 and Hazrat Usman5 

...ry were used for mineral analysis. Total phenolic compounds were evaluated by the Folin-Ciocalteu method. The aluminum chloride colorimetric method was used for evaluating total flavonoids contents. Antioxidant activity was assessed by the DPPH method. The results showed that moisture was high in Buttermilk (92.15±0.13 g/100g), ash and proteins in Manpasand (5.71±0.03, 25.36±0.04 g/100g respectively), carbohydrates in Mardani Paida (79.69&...

Othman E. Othman1*, Lingjiang Min2, Amira M. Nowier3  

...or functional enrichment analysis and the results declared the strong relation between methylation patterns of DMGs and fertility trait. It is concluded that DNA methylation patterns of Zaraibi goat ovaries may be responsible for difference in ovulation rate trait between high and low fertility groups through their important roles in folliculogenesis and oocyte ovulation rate. Also, this study declared the opposite association between the methylation levels an...

Sonia Kanwal1, Muhammad Naeem1*, Zaib Un Nisa1, Rabia Farhat1 and Zia Ur Rehman2

...ortisol. The statistical analysis was done by two way ANOVA and Duncan Multiple Range Test (DMRT). T3 and T4 showed significantly different results  during various stages of lactation in Lohi sheep and Beetal goats. However, Vitamin E, Vitamin C and Cortisol did not reveal significantly different results during lactation in both ewes and does. It could be postulated that the evaluation of vitamins and hormones during various stages of lactation were good ...

Yêyinou Laura Estelle Loko1*, Joelle Toffa1, Azize Orobiyi1, Gbèblonoudo Anicet Dassou2, Rolande Okpeicha1, Dieudonné Gavoedo1 and Alexandre Dansi2

...elation path coefficient analysis. The tested soybean varieties showed a variation in physical seed characteristics. A differential susceptibility of soybean varieties to C. maculatus was observed, with the white seeded variety Whéwhé having longest and largest grains was the most susceptible. Based on the Dobie susceptibility index, the Yovoton variety was proved to be resistant to C. maculatus attacks. While, Kecheke, Houeton, Adjaton and Vovoh...

Ghulam Qadar* and Khalid Nawab

...ogical competencies. PCA analysis also showed that high age, more service length and diploma holder category of agriculture school teachers has negative significant correlation with teaching methodological competencies. It is concluded that the AJandK education department may positively be considered Refresher courses and new teaching staff in the agriculture education system of education department.

...

Ishtiaq Ahmad1,2* and Muhammad Akbar Anjum1

...analyzed in multivariate analysis. Cluster analysis indicated that 78 genotypes were grouped into five clusters where cluster-I comprised of (19) genotypes, cluster-II contain (8) genotypes, cluster-III contain (21) genotypes, cluster-IV contain (6) genotypes and cluster-V contain (24) genotypes. PCA analysis based on first two components for morphological attributes of Capsicum frutescens...

Yue Ren1, Ting Jia2, Hao Zhang1, Zhengkun Wang1 and Wanlong Zhu1*

Hasan Çelikyürek

...o the results of the GLM analysis that birth type had no significant influence on testicular traits or on any of the body measurements except body length. Live weight gain had significant impact (p<0.001) on all traits. Age also had significant impact on lambs’ body length, chest width, and chest depth (p<0.001), a significant effect on rump height and chest girth (p<0.01), and a significant effect on withers height (p<0.05).

...

Muhammad Jamil1*, Muhammad Ihsan Ullah2, Taj Muhammad2, Syed Waqar Hussain Shah3, Khezir Hayat4, Muhammad Zahid Aslam5 and Abdul Sattar6

...erval of two weeks. Data analysis depicted that sowing dates influenced all study traits significantly except for staple length. Similarly, genotypes and interactive effects were found significant. Genotype VH-351 yielded the highest (2523.3 kg ha-1) when sown in 1stMarch, while VH-402 gave the lowest seed cotton yield (209 kg ha-1) on the planting date of 16th June. The finest lint-bearing micronaire value (3.9656) was obtained by normal season sowing in 1stM...

Khalid Khan1*, Mahmood-ul-Hassan2, Ihsan Ullah1, Haris Khurshid1, F.Y Saleem Marwat1, Salman Saleem1, M. Jehanzeb1 and Zubair Ahmad1

...ological traits. Cluster analysis, based on morphological traits revealed five major groups. Maximum cluster distance was observed in group I and V, while minimum cluster distance was observed in group IV and V. Highest emergence (33.7 m-2) was noticed for ICSA220XICSR90, highest leaves per plant (11.68) were reported of hybrid combination ICSA220XICSR90, maximum leaf to stem ratios (0.02) was recorded for hybrid combinations ICSA233XICSR90, high stem weight (...

Jiangbo Shao1, Donglai Zhu2, Shengqiang Zou1, Guohong Ge1 and Ju Huang1*

...rehensive bioinformatics analysis. We found that SOX9 mainly participated in heat stress response, protein folding as well as metabolism and biosynthesis of amino acid. SOX9 correlated with HSPA1B were negative to the progression in hepatocellular carcinoma patients. These results suggest that SOX9 participated in hepatocellular carcinoma progression through targeted regulation of HSPA1B that might provide novel insights in hepatocellular carcinoma therapy.

Zahin Anjum1*, Mubashra Tarana1, Shaista Ali1, Faryal Yousaf1, Amina Rahat1 and Sumbla Yousaf2

...Pb and Ni). In addition, analysis of five electrolytes (Ca, Na, K, P and Mg), moisture content, protein, lipids and ash were carried out. The samples were obtained from five different sites of the University Campus, Peshawar- Pakistan. The Atomic Absorption Spectrometry was employed for determination of Cu, Zn, Pb, Ni, Ca and Mg. Furthermore, a spectrophotometer was also used for the purpose of phosphorus (P). However, the analysis

Sajida Batool*, Sitara Shameem, Kainat Malik and Aneela Iram

...cessed feed. Statistical analysis revealed that the average cross-sectional area (ACSA) of Bowman’s capsule was significantly (P<0.05) increased in mice raised on feed that is directly exposed to microwaves radiations while other groups showed no significant change in Bowman’s capsule as compared to control. ACSA of glomeruli and renal tubules significantly increased in direct (P<0.001) and in the plastic (P<0.01) group as compared to the ...

Zheng-Yong Wen1, 2*, Chuan-Jie Qin1,2, Bin Li1,2, Rui Li1,2 and Xiao-Tao Shi3*

...trapods. Genetic synteny analysis revealed that lepB had specifically lost in siluriformes teleosts. Phylogenetic analysis showed that fish lineage contained two clades of leptinA and leptinB, and the pvleptin was grouped into leptinA clade and shared a close relationship with its counterpart in P. fulvidraco. Tissue distribution analysis showed that pvleptin was widely distributed with th...

Sekobane Daniel Kolobe, Tlou Grace Manyelo*, Jones Wilfred Ng’ambi, Munyadziwa Felicia Dorcus Nemauluma, Emmanuel Malematja  

Qamar Javed Iqbal1*, Zainab Masood2, Asad Abbas3 and Sidra Munir4

...was accomplished by FTIR analysis which shows peak of epoxy group at 845cm-1.

...

Lobna M.A. Salem, Nashwa O. Khalifa, Marwa O. Abd El-Halim* 

...cies was examined by the analysis of forward and reverse sequences of the ITS2 of the rDNA gene that amplified in 300bp. Out of 286 slaughtered animals {88 sheep, 26 goats, 68 cattle, 25 buffaloes and 79 camels} in the regions of Qualyobia, 51 (17.8%) had Fasciola spp. morphologically {27/51(52.9%) were identical to F. hepatica while 18/51(35.3%) were identical to F. gigantica, 3/51(3.9%) were larval stages and 3/51(3.9%) mixed infections}. DNA from 10 flukes ...

Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4

... RT-PCR and Western Blot analysis showed that the expression of TNF-α, IL-6, IKKβ, NF-κB increased significantly after the establishment of the model; after the administration of DHEA, the levels of TNF-α, IL-6, IKKβ, NF-κB were down-regulated, and the difference was statistically significant compared with the model group (P<0.05). It is concluded that DHEA could regulate estrogen levels in rats through the estrogen-intesti...
Farzana Bashir2, Aysha Saleem1, Khalid Iqbal Khichi2, Rubina Nelofer3* and Rauf Ahmad Khan2
...were tested by means the analysis of variance (ANOVA) with 95% confidence limits. The antibacterial susceptibility was optimized for flow rate, bacterial count and column height. High regression coefficient between the variable and response (R2=99.4%) showed good evaluation of experimental data by polynomial regression model. The optimum conditions suggested by the model for the variables such as flow rate, bacterial load and column height were 46 ml/min, 262 ...

Syeda Batool Zehra1, Abdullah G. Arijo2, Aly Khan3, Nasira Khatoon1* and Samina Waheed1

...o key factors: molecular analysis and statistical analysis. When traditional morphological techniques used with, molecular biology technologies it has proven to be effective in distinguishing closely related species. Statistical analysis is used to determine the intensity of infection in relation to age and gender. This study will focus on children who are suspected of being afflicted with...

Jie Li, Gaofu Wang, Xiaoyan Sun, Lin Fu, Peng Zhou* and Hangxing Ren*

... and immunohistochemical analysis. This study provides a theoretical basis for further clarifying the biological function of the KITL gene in goat.

...

Murad Ali Rahat1*, Muhammad Israr2, Hakim Khan1, Ishtiaq Hassan1, Mohammad Islam1 and Muzafar Shah3

... samples collection. The analysis showed that brown eye color individuals were greater in number than blue and intermediate eyes color individuals. Moreover, Irisplex system predicted 100% blue and brown eye color while issue in predicting in intermediate eye color. The data of the current study indicated the six Irisplex SNPs the rs16891982 and rs1800407 SNP are significant association with eye color. Conclusion: This study showed that the most prevalent huma...

Man Wang1 and Fengmei Yang2*

... based on bioinformatics analysis. GEPIA, UALCAN, cbioportal and other tools were used to analyze the correlation between TOP2A gene expression and methylation, prognosis and immune cell infiltration in databases. The expressions of TOP2A gene and protein in tumor tissues were higher than those in normal tissues, and the differences were statistically significant (P<0.05); TOP2A gene expression was positively correlated with immune cells infiltration (P<...

Ishtiaq Ahmad1*, Muhammad Nafees1, Maryam2, Irfan Ashraf3, Ambreen Maqsood4 and Muhammad Saqib5 

Ty Nguyen, Giang Thi Huong Phung, Lan Le Thuy Hoang and Giang Van Tran*

...morphometric and genetic analysis of M. cunnesius in this study contributed the documents for identification and the genetic diversity, which are the basis for further research about M. cunnesius in breeding, species conservation and management at the Tam Giang Cau Hai Wetland Reserve. Research results showed that this species distributed in the North has a size ranging from 101.7 to 160.7 mm, with an average of 137 mm, corresponding to a weight of 10.6 - 35.6...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

...lected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best concentration of sericin and curcumin was successfully achieved and was used in further studies. In the group that was given carrageenan alone, the number of erythrocytes, hemoglobin and platelets were decre...

Sehrish Ashraaf1, Hafiz Muhammad Tahir1*, Chand Raza1, Ayesha Muzamil1, Farman Ahmad Chaudhary2, Muhammad Summer1 and Aamir Ali1

...lected for hematological analysis to observe the mean count of erythrocytes, hemoglobin, platelets, white blood cells (neutrophils, monocytes, eosinophils and basophils) in control and experimental groups. Based on the preliminary results a mixture of best concentration of sericin and curcumin was successfully achieved and was used in further studies. In the group that was given carrageenan alone, the number of erythrocytes, hemoglobin and platelets were decre...

Sherif Abdelghany1, Hossam Mahrous Ebeid2*, Ahmed Abdelkader Aboamer2, Mohamed Ali Radwan1, Rania Agamy1 

...a through survey and lab analysis for milk (Fat: protein ratio and MUN) and feeds samples could help to set new and practical standards that fit small-scale farms at the districts level as well as adapt commercialized diagnostic tools for feeding practices in rural areas.

Keywords | Rural Regions, Dairy Farms, Feeding Practices, Milk Analysis, Milk urea nitrogen, Smallholders 

...

Mahmut Islamoglu

... the multiple regression analysis, it was determined that the equation Y = -132.796 + 02.233 T + 11.646 P [where Y= Sunn pest (Number), T= Temperature, P= Photoperiods (H)] is appropriate. This model was determined to have the best correlation (r = 0.755, r2 = 0.571, n = 122, P = 0.000). Moreover; It is determined that air temperature and photoperiod affect the migration of Sunn pest positively, while other parameters are not statistically significant.

...

Widiastuti Ardiansyah1,3, Osfar Sjofjan2*, Eko Widodo2, Suyadi Suyadi2, Danung Nur Adli2 

... comprised a descriptive analysis and a completely randomized design with six treatments and four replications with eight Mojosari laying ducks in each experimental unit. An extraction and maceration were employed during the early screening of the bioactive compound of turmeric. The treatments consisted of T0 = basal diet, T1 = basal diet + 0.2% turmeric, T2 = basal diet + 0.8% turmeric, T3 = basal diet + 0.2% turmeric + 0.1% probiotics, T4 = basal diet + turm...

Ansar Abbas1*, Muhammad Aman Ullah2, Abdul Waheed3 and Muhammad Asif4

...However, the comparative analysis shows that the CHAID algorithm performed well as compared to the MLR (CHAID vs. MLR: R2 % = 79.38 vs. 73.27; Adj-R2 %= 78.70 vs. 72.50; SD ratio=0.45 vs. 0.51; RMSE= 3.15 vs. 3.58; RAE= 0.12 vs. 0.14; MAD= 2.41 vs. 2.74; CV%=13.28 vs. 15.11; AIC =638.50 vs. 708.28; r= 0.89 vs. 0.85). Consequently, it is hoped that the results of the study on the morphological characterization of Thalli sheep might be a good reference for next ...

V. Sunitha*, V. Vasudeva Rao, V. Ravinder Reddy, P. Venkateshwarlu, A.V.L.N. Ramalingeswar Rao and B. Naresh

...recorded. The month wise analysis for the year 2015-17 showed that the diet of the spotted owlet closely related to availability of prey in the study area. The occurrences of insects were dominant during the month of April to September and from October to March the rodents were predominant. The overall analysis showed that the spotted owlet diet consists of predominantly insects followed by rodents, reptiles, amphibians (Opp...

Jianmin Liu1, Jiancheng Zhang2, Peijuan Wang3,* and Moslem Lari Najafi4

...30 cases). A comparative analysis is made on sex hormone levels, ovarian artery blood flow, ovarian volume and number, treatment efficacy, glucose metabolism, ovulation and fertility in each group. There are statistical differences in sex hormone level, ovarian arterial blood flow, ovarian volume and number, treatment efficacy, glucose metabolism, ovulation and fertility in each group (P<0.05). The total effective rate of new addition congrong tusi pill is ...

Muhammad Mansoor1, 2, Sheheryar1 and Shahid Ali Khan1*

...aximum canopy level. The analysis was carried out in randomized complete block design (RCBD) with three replications of split plot arrangement; varieties (NM-2011, Inqelab Mung, Dera Mung, and NM-98) were allocated in the main plot and sub-plots were held at various flowering flushes. The analyzed data revealed that the percentage of pods plant-1, weight pod-1, 1000 grain weight, grain yield, and harvest index were significantly affected by different varieties...

Muhammad Mansoor1, 2, Sheheryar1 and Shahid Ali Khan1*

...aximum canopy level. The analysis was carried out in randomized complete block design (RCBD) with three replications of split plot arrangement; varieties (NM-2011, Inqelab Mung, Dera Mung, and NM-98) were allocated in the main plot and sub-plots were held at various flowering flushes. The analyzed data revealed that the percentage of pods plant-1, weight pod-1, 1000 grain weight, grain yield, and harvest index were significantly affected by different varieties...

Bilal Saeed Khan1*, Muhammad Arslan1, Abdul Ghaffar2, Muhammad Farooq2, Saghir Ahmad3, Sami Ullah4 and Awais Rasool5

... The pearson correlation analysis demonstrated that most mite families had a negative correlation with morning and evening relative humidity while the positive correlation with maximum and minimum temperature. The results showed higher Shannon diversity index was observed in Mana Ahmadani during October and February (2.04 and 2.03, respectively) whereas the least value was observed in Basti Halla during February (1.60). Finally it could be concluded that farme...

Neni Widaningsih1,2*, Budi Hartono2, Hari Dwi Utami2, Eni Siti Rohaeni3 

...persons. Methods of data analysis carried out include; qualitative analysis and quantitative analysis. Qualitative analysis is intended to find out the characteristics of marketing institutions, and the marketing channels of buffalo cattle. While quantitative analysis is intended to find out margins and farmer share an...

Hams M.A. Mohamed1*, Katreen K.G.2, M.W. Abd Al-Azeem1, Faysal A. Wasel2, Ahmed M. Abd-Eldayem3 

...ted to 16S rRNA sequence analysis and identified as L. innocua (three isolates) and L. welshimeri (two isolates). The antibiotic susceptibility profiling revealed multidrug resistance of L. monocytogenes strains against several antimicrobials in addition, they harbored antibiotic resistance genes, including. ampC, aad6, tetM each present in 100% of our isolates and mefA (37.5%). Furthermore, different virulence genes, including the most often found virulence-a...

Amber Khalid1*, Farah Ashfaq1, Shahzadi Farah1, Amna Akbar1, Farkhanda Manzoor1, Muhammad Babar Khawar2, Saira Riaz3 and Safina Ashfaq3

...tinine and Serum albumin analysis was done by using automated chemistry analyzer. Results concluded that the experimental subjects have shown a significant difference (P<0.00) in HDL level as compared to control subjects. Result of t test has shown significant increase (P<0.00) in total cholesterol and triglycerides level. Results have shown that there is highly significant correlation between body mass and serum creatinine level as P < 0.05. Serum al...

Muhammad Jamil1*, Rao Sohail Ahmad Khan2, Rana Abdul Hameed Khan3, Khezir Hayat4, Saeed Ahmad5, Abdul Sattar6, Umair Faheem7, Hira Saher8 and Ayesha Bibi8

...ative interaction (AMMI) analysis results depicted that maximum variability was due to environments (63.9%) followed by GEI (24.1%). The first two interaction principal components (IPCs) were squeezed with 75% of variability due to GEI. The genotype GN01 (SLH-Chandi) produced the maximum seed cotton yield (2181 kg ha-1) and was the winner in this trial. The site EN03 (Faisalabad during 2020-21) produced the highest seed cotton yield (2541 kg ha-1) with a (-6.8...

Hafiz Ghulam Muhu-Din Ahmed1*, Aziz Ullah2, Muhammad Asim Bhutta3, Ammara Yasmeen4, Hafeez ur Rehman5 and Umar Farooq5

Humaira Gul1*, Muhammad Junaid Yousaf1*, Fawad Ali1, Mamoona Rauf1, Farhad Ali2 and Iqthedar Ali3
...oportion whereas in soil analysis moisture, hygroscopic water, pH, cation exchange capacity (CEC), chlorides, organic matter were significantly (P<0.05) increased for the soily soil in same proportion that is of sandy soil. This reveals that Barley should be grown in sandy soil for its better growth and productivity. The least growth parameters were noted on soil with more clay retaining most of moisture and inhibiting growth due to soil pH.

...

Naheed Zahra*, Muhammad Qasim, Muhammad Azam Niazi, Muhammad Ishaq and Mubbashira Nazir

Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1 

...followed by phylogenetic analysis according to the year examined, mortality rate, positive results of RT- qPCR with low Ct value (H5 and N8 were 15 and 20 respectively). The phylogenetic tree revealed that H5N8 field isolate clustering in clade 2.3.4.4b. The histopathological hallmarks, in H5N8 naturally infected ducks, included acute inflammatory, necrotic and vascular reactions that involve different tissues. The immunohistochemical characterization of viral...

Muhammad Nadeem1, Muhammad Rizwan2,3*, Tanveer Ahmad4, Muhammad Kashif5, Aneela Zameer Durrani2, Muhammad Ali6, Asghar Abbas7, Safder Imran8, Sidra Saher9, Syeda Fakhra Waheed10

...surveillance (PDS). Data analysis showed that FMD, theileriosis, and HS are the most important, of which FMD was found to be most common diseases in study area. Other main livestock health problems observed during study was respiratory syndrome, mastitis, enterotoxaemia, tick and mite infestations and pox disease. There was no proof of rinderpest occurrence since long in area of examination. Participatory disease surveillance presented to be a worth full techn...

Naila Shahzadi1, Hafiz Muhammad Tahir1*, Azizullah1, Muhamamd Farooq Bhatti2, Shaukat Ali1, Muhamamd Summer1 and Farman Ahmad Chaudhary3

... is first of its kind of analysis for feed supplements of natural products such as sericin and honey have improved the quality and quantity of silkworm and silk cacoons.
...

Samantha Shiuan Erl Lee1, Nurul Huda2*, Yetti Marlida3, Eng-Keng Seow4 

...trength, texture profile analysis, foaming capacity and stability, and emulsification stability were compared. Ultrasound treatment had caused a significant decrease (p<0.05) in pH and expressible moisture, apart from improving the gel strength and folding test score of egg white gels. Improvement of gel strength was substantiated by the significantly higher (p<0.05) hardness and springiness of egg white gels. Hardness and springiness of ultrasound-treat...
Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3
Shahrokh Mojarradgandoukmolla* and Hasan Akan
...h ethanol. Phytochemical analysis of seeds and leaves of these taxa was performed by GC-MASs and their morphological evaluation was performed by SEM electron microscopy. Hematological and biochemical variables such as hepatic enzymes activity, glucose, lipid profile, ESR, CRP, renal function tests, and oxidative biomarkers were measured. Histological evaluation was also performed. Among the hematological parameters in the treated groups, only lymphocytes showe...

Khunsa Khakwani1*, Muhammad Asif1, Zaheer Ahmed2, Ghulam Sarwar1, Yasmeen Abbassi2 and Ghulam Murtaza2 

Mochamad Rizal Umami1, Zainuri*2, Sebastiana Viphindrartin2 and Rafael Purtomo Somaji2

...tive methods and content analysis. The study found that the COVID-19 pandemic formed a new pattern of sugarcane trading system, switching from a contractual trade model to a free market model (redeemer). The model refutes the theory stating that the contractual model will be more efficient than the free market model due to its lower transaction costs. The proposition (substantive theory) produced in this study states that in conditions of sugarcane commodities...

Jinzhao He1, Pengfei Feng2, Junqi Qin2, Yun Teng1, Xu Luo2*, Zigui Chen1*, Huawei Ma2* and Dayan Zhou1

...omparative transcriptome analysis was used to screen the candidate genes linked to the skin color differentiation of pearl white red tilapia. Among them, the body color of Guam red tilapia changed when the water temperature dropped to 16-14°C, and continued to drop to about 11°C, it was discolored in a large area reaching above 90%. According to the differential analysis: Tyrosine Kinase STYK1, HSP70, HSP30 and Trans...

He-Cai Zhang, Tian-Ge Hu, Chang-Ying Shi, Guang-Wen Chen* and De-Zeng Liu

...ip and haplotype network analysis indicated that there was no obvious correspondence between the haplotypes and the geographical locations, revealing no obvious phylogeographic pattern. Analysis of molecular variance (AMOVA) suggested that the genetic differentiation of D. japonica in Taihang Mountains was very great (FST=0.405, P<0.01). This significant genetic variation mainly occurred within populations (59.52%), follo...

Uzma Zulfiqar1, Mahroze Fatima2, Syed Zakir Hussain Shah3*, Muhammad Afzal1, Shazia Shamas3, Sadia Roshan3, Moazama Batool4 and Muhammad Bilal5

...l in the diet. Moreover, analysis of fatty acids profile showed decrease in saturated and monounsaturated fatty acids (MUFAs). While, polyunsaturated fatty acids (PUFAs) were increased in all vitamin E supplemented diets compared to control group. Furthermore, vitamin E level of 80.62 mg/kg was found optimum for maximum growth performance in L. rohita fingerlings, beyond which no increase in growth was observed.

...
Dong-Min Hou1, Ting Jia2, Hui-Juan Wang1, Zheng-Kun Wang1 and Wan-Long Zhu1*
...ed out by bioinformatics analysis. The results showed that there were significant differences in the expression of 2879 genes in cold acclimation group compared with that of control group, 1181 were up-regulated and 1698 were down-regulated in cold acclimation group. Pathway analyses showed that the differentially expressed genes were significantly enriched in oxidative phosphorylation, steroid biosynthesis, glycerophospholipid and fatty acid metabolism pathwa...

Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*

...ollected for biochemical analysis, and sex organs were dissected out for histological preparations. Administration of testosterone has significantly increased the body weight and decreased the gonad weight in both sexes. It drastically affected the histology of testes and ovaries, causing a reduction in the sperm count and motility with a parallel reduction in the acid phosphatase activity. It also directly reduced the concentration of free testosterone and es...

Muhammad Saif-ur Rehman1, Faiz-ul Hassan1* and Muhammad Sajjad Khan2

...ttle breed. Phylogenetic analysis showed the relatively high similarity indices among 10 cattle breeds (ranging from 83.85% to 89.62%). The highest similarity was observed between Dhanni and Lohani (89.62%), followed by Sahiwal and Red Sindhi with 89.44% similarity. However, the lowest similarity was observed between Cholistani and Tharparkar (83.85%). The cluster analysis of UPGMA showed two major clusters: Cluster A having...

Saima Rubab1,3*, Ghazala H Rizwani2, Arjumand Iqbal Durrani3, Iram Liaqat4*, Urooj Zafar5, Mahjabeen6, Farah Batool7, Noor-E- Seher3, Naveera Younas3 and Ayesha Sadiqa8

...and water. Phytochemical analysis of all extracts showed the presence of polyphenolic compounds, flavonoids, alkaloids, glycosides and carbohydrates in all plant parts with varied strength. Phytochemical analysis showed comparatively high percentage yield of ethanolic extract of all plant parts, hence was employed for evaluating the antimicrobial potential against 11 Gram positive bacteria, 9 Gram negative bacteria, 2 yeasts...

Ahmed Fathy1, Rania A. Hassan2, Essam S. Soliman3* 

...care. Since discriminant analysis as a parametric test is more powerful than its non-parametric alternatives, we aimed to evaluate the performance of the single discriminant analysis in classification between Baladi buffaloes and crossbred using clinical epidemiological measures of some productive and reproductive traits such as milk yield (MY/kg), days in milk (DIM/days), age at first calving (AFC/months), calving interval ...
Chunlan Shan1, Chaoying Liu1, Qin Lu1, Guowen Fu2, Syed Aftab Hussain Shah3, Rana Waseem Akhtar4, Ru Zhao2, Libo Gao2, Chang Liu2, Shushu Miao2, Hongdan Wang2 and Hong Gao2*

 

Amal G. Abdelrahman1, Nabil A. Yassien1, Hussein M.H. Mohamed1, Khaled S. Tolba2, Heba H.S. Abdel-Naeem1* 

...ed to proximate chemical analysis, measurement of deterioration criteria, and sensory quality. The results revealed that all enzyme-treated samples significantly increase protein content and significantly decrease the fat content, pH, and thiobarbituric acid value with a significant improvement in raw and cooked sensory attributes as compared to their counterpart control samples. Such effect was more pronounced in spent hen meat patties treated with a mixture ...

Ilhama G. Kerimova1*, Viktor A.Krivokhatsky2, Merve N. Aydemir3 and Lala N. Mamedova4

... first report on the DNA analysis of the lacewing insects of Azerbaijan. A total of 25 species of antlions, mantidflies and owlflies were DNA barcoded.
...

Muhammad Azam1*, Shaista Naz1*, Malik Muhammad Shafi2, Muhammad Jamil Afridi3 and Ahmad Waheed4

...ical techniques for data analysis. It has been found that majority of the farm households (65%) were food secure, still the issue of food insecurity prevailed among the 35% of the farm households. The positive determinants included family type, monthly income, and small ruminants, while tenancy and household size were found as the negative determinants of food security. The issue of food insecurity has been coped through the adoption of various strategies like...

Adrial Adrial1*, Rudy Priyanto2, Salundik Salundik2, Ahmad Yani2, Luki Abdullah3 

... supported by laboratory analysis. Reproductive performance data were obtained through direct interviews and recording cards. Soil and forage samples were taken from the main forage source locations. A total of 138 blood samples were taken through the jugular vein from Bali cows and their crossbred cows with different physiological status. Soil and blood serum samples were analyzed in laboratory using Atomic Absorption Spectrophotometry (AAS) method, while for...

Müge Aliye Hekimoglu and Sırma Sönmez

...survival rate. The image analysis method was used to determine skin colour. The chemical analysis method was used to determine the activity of enzymes. The results showed that supplementation of 2000 mg/kg of dietary significantly increased weight, length and specific growth rate (P < 0.05). While no change in colour was observed in the control group at the end of the experiment, it was determined that carnitine could hav...
Xiao Zhang1, Guoqing Shen1, Yang Wang1, Pengdan Huang1,2, Yan Shui2, Zenghong Xu2 and Huaishun Shen1, 2*
...ng data. Based on a Kmer analysis, the P. clarkii genome size was projected as approximately 3.6 Gb. A total of 336,275 SSRs (simple sequence repeats) identified motifs were identified. There were 167,999 the most common type di-nucleotide repeat motifs (49.94%). The second most common repeats were the tri-nucleotide ones (131,405, 39.09%). A total of 312,454 microsatellite loci had flanking sequences. A subset of 56 primer pairs was randomly selected for the ...

Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza

... test, PCR, and SDS-PAGE analysis for the confirmation of methicillin resistance. The farm-associated and individual animal-associated risk factors were analyzed to check the association with S. aureus and MRSA-associated subclinical mastitis. The results revealed an overall molecular prevalence of 28.70% for S. aureus among which MRSA-associated mastitis was found 47.62% prevalent. The SDS-PAGE analysis depicted the presenc...

Carla Meneguin Barbosa1*, Fabio Gregori2, Cairo Monteiro1, Thais Helena Martins Gamon1, Luciano Matsumiya Thomazelli1, Tânia de Freitas Raso2 and Edison Luiz Durigon1

...uencing and phylogenetic analysis support these results.

...

Barbosa Carla1*, Gregori Fabio2, Thomazelli Luciano1, Oliveira Amanda1, Araújo Jansen1, Ometto Tatiana1, Marcatti Roberta3, Nardi Marcelo3, Paludo Danielle4, Utecht Nathalia1 and Edison Durigon1

...ubmitted to phylogenetic analysis. From total of 8 confirmed samples, 3 clustered to Deltacoronavirus genus and 5 to Gammacoronavirus. Two of those samples showed partial sequences of other regions of CoVs confirming their genotyping to Gammacoronavirus genus and proximity to important poultry diseases causative agents.

...

S. Samina and Y.I. Erum†

... Allen, 1950. Population analysis of nematode fauna encountered from Kurram Agency was also determined.

...

N. Hajra

...curbitaceae. Biochemical analysis including carbon and nitrogen profiles was taken into account.The estimation of carbon profile comprising of carbohydrate, glucose, sucrose and total soluble sugars and nitrogen profile comprising of proteins, amino acids protease and proline. Results showed that carbon profile in plant treated with AM fungi have high to low and varied amount of carbohydrates, sucrose, glucose and total soluble sugars in different parts of the...

H. M. Hassan, M. M. Tantawy*, A. M. Younes and M. O. Sayed

...plolaimus spp. Community analysis of these plant parasitic nematodes showed variability in nematodes population densities, frequencies, biomass and importance values in different Districts during 2012 and 2013. Root-knot nematodes Meloidogyne spp. was the most prevalent genus in the entire plant parasitic nematode community having highest relative frequency (0.78 and 0.83) in both years, respectively. The lowest relative frequencies (0.31 and 0.40) were record...

W. M. El-Nagdi,1†, Z. , E. Ghareeb2 and E. M. Zayed3

...ltiple regression linear analysis for fodder beet yield showed that root weight per plant, gall index, number of leaves per plant and dry weight per plant were the most important contributing traits to root yield (R2= 94.76%). Hence, the selection among these traits would be accompanied by high yielding and more effective for the improvement of fodder root yield in the same conditions.

...

Urooj Shakoor* and Syed Attaullah Shah

...thod, and a binary logit analysis was conducted to identify its determinants. Data set of 3035 rural households, extracted from Pakistan Social and Living Standard Measurement survey (2018-19), was grouped into three geo-climate zones (Northern, Central and Southern zones). Results from data analysis revealed that 46.6 percent of the sampled rural households were multidimensional poor and the derived Multidimensional Poverty...

Shafia Riaz1, Khalid Abbas1*, Tanveer Ahmed1,2*, Huma Naz3, Hina Amjad1 and Shahbaz Ahmad1

...tal length (mm). Genetic analysis revealed significant differences based on allelic frequencies, heterozygosity, inbreeding and genetic distance among hatchery genotypes. Impact of G×E interaction is illustrated by results where genetically diverse FQB genotype performed maximum under similar cultural environment among all genotypes.

...

E. Mahdikhani-Moghadam†, J. A. A. Bub, S. B. Chery and S. Alvani

...as of the province. Soil analysis results in the identification of 5 species of mononchid
nematodes viz., Anatonchus kashmirensis, A. tridentatus, Clarkus papillatus, Mylonchulus brachyuris and M.
paitensis. Among them, A. kashmirensis is recorded for the first time in Iran.
...
A.M. El-Baha1, A. A. El-Sherbiny2†, M. Z. M. Salem1, N. Shaarawy3 and N. H. Mohamed3
...ing J2 viability. Probit analysis results showed median
inhibitory concentration (IC50) values of 412.7 and 615.9 mg/l for C. citriodora and E. camaldulensis leaves EOs,
respectively. In addition, the lethal concentrations (LC) causing 50% J2 mortality (LC50) of C. citriodora and E.
camaldulensis oils were 235.9 and 327.7 mg/l, for C. citriodora and E. camaldulensis leaves EOs, respectively.
Essetial oils were an...
K. Nasira, S. Shamim and F. Shahina†
...itoring. Detailed sample analysis revealed the presence of forty nematode species
including three new species viz., Bathyeurystomina minima Nasira, Shahina & Shamim, 2014, Belbolla
longispiculata Nasira, Shahina & Shamim, 2014 and Metoncholaimus siddiqii Shahina, Nasira & Shamim, 2015
while six species were reported as new records of Pakistan. These nematode species belong to 36 genera, 27 families
an...

H. Ravindra1, N. Adivappar2, M. Sehgal3 D. M. Soumya4 and H. B. Narasimhamurthy4

...mples were collected for analysis of nematode infestation. Coriander
plants also exhibited poor growth showing stunting and chlorosis due to severe infestation. This is the first report of
root-knot nematode from Karnataka.
...

K. Nasira† and S. Shamim

...ng sites of Sindh. After analysis it was found that different parameters of
community structure of fresh water nematodes varied considerably at different surveyed sites. Nematode community
analysis revealed that frequency (absolute frequency, relative frequency), density (relative density) and prominence
value were variable for nematode population within localities. Relative density of fre...
A.W. Aseffa1, F. F. Addisu2, G. N. Roge3, L. T. Hadis4, T. B. Abera5, M. G. Gero6 and B. H. Meressa1†

S. Anwar1, K. Wieczorek1 and E. Inselsbacher2

...y, a detailed amino acid analysis based on Ultra Performance Liquid Chromatography (UPLCTM) was performed. The results showed significant increase in arginine, proline and glutamine amounts in syncytia. Ammonium ions were also increased as an important precursor in arginine, proline and glutamine biosynthesis. In addition, branched chain amino acids and amino acids of shikimic acid based pathway were also enriched in syncytia. This study opens a door to unders...

Shaista Naz1*, Muhammad Arif2 and Akhtar Ali3

...d were reviewed. Content analysis has been used for analysing data and the study found that women have higher participation in livestock management as compared to crop production due to the easy accessibility of livestock and the quick outcome in the shape of milk and milk products for home consumption and sale to earn cash. Furthermore, women were found to have higher participation in the indoor livestock management activities like animal feeding, serving wat...

Muhammad Nadeem1*, Aneeta Rehman1, Masooma Munir1*, Hira Fatima2, Faiqa Malik1, Aqsa Iqbal1 and Alaiha Asif3

...l, microbial and sensory analysis characteristics. The proximate analysis of date bars supplemented with water chestnut indicated that the moisture, crude protein, fat, fiber, ash and NFE contents for treatments ranges from 5.45-7.27%, 4.02-5.25%, 1.77-2.81%, 1.48-2.97%, 1.28-1.93% and 82.69-83.09%, respectively. The microbial analysis of fruit bars showed that mean values of TPC changed g...

F. Shahina† and G. Mehreen

...e examined using genetic analysis by ITS
rDNA and 12S mtDNA. Phylogenetic analysis of these isolates was inferred by using four different methods i.e.,
Maximum Evolution (ME), Maximum Likelihood (ML), Maximum Parsimony (MP) and Neighbor Joining (NJ)
based on the two makers. Sequence composition and phylogenetic analyses of these isolates showed closeness with
Steinernema  ...

S. Ahmed†, A. Munir, S. Hameed, S. Asad, M. Fayyaz, M. Zakaria and M. Umer

S.A. Khan†, M. Abid and F. Hussain1

...een Ward’s cluster analysis of hatching egg and larval mortality in water and methanol extract of
different seaweeds showed significant difference. Methanol extract was more effective as compared to water extract.
In conclusion, use of seaweeds is positive sign against harmful microorganisms which are responsible for
considerable losses in agriculture yield.
...

D.A. Darban†, S.R. Gowen, B. Pembroke, F. Hussain1 and R.A. Memon2

...nto the soil. Regression analysis was
carried out to compare the degradation of P. penetrans infected females cadavers in different time intervals over two
crop cycles. There were highly significant decreases in the total number of egg-masses per plant between the 3
weeks and other treatments. Infected females (%) over both crop cycles were compared by the estimated coefficients
of th...

O. A. Fabiyi

... mortality. The infrared analysis
of the fractions revealed the presence of anhydride and carbonyl stretching frequencies at 3638, 1702, 1735 and
1771 cm-1. The Nuclear Magnetic Resonance (NMR) of the fractions showed signals that agreed with ozoic and
daniellic acids. Preliminary phytochemical screening of the leaves revealed the presence of flavonoids, steroids,
terpenoids, reducing sugars, phenols, alkaloids, ...

Belema Robert1, Favour Welenya1, Deborah Achi1, Cynthia Onyeagwara1, Soala Obie Minimah2, Ebele Anulika Obichi2, Chidinma Charity Amuzie1* 

...eni.
Statistical analysis: Prevalence of infection was computed following standard formula. Recent finding and conclusion: It is concluded that waterleaves sold in and harvested from the selected locations are commonly infected with S. stercoralis larvae. It is hence recommended that special care be taken in the cleaning and washing of this vegetable, possibly with saline water, to limit the chances of human infection.

Keywords | Strongyloidias...

Jax Vincent Gamulo, Maye Pearl Bolina, Jessica Serena Brion, Via Crishiela Nicole Dela Rosa, Roxanne Francesca Maglaya, Carl Lexter Tan, Aimee Caye Chang* 

...tematic review with meta-analysis (SR-MA) explored the potential of phytotherapy against fascioliasis and as alternative to commercially available anthelmintic drugs. Eligibility criteria for inclusion and protocol was defined for systematic publication database searching. Final reference database consisted of eight (8) published journal articles with a total of 106 Fasciola flukes, published between years 2001 to 2021. The mortality time between tropical plan...

Yuejing Yang1,2, Mengbin Xiang1,2, Zhengshi Zhang3, Minrui Zhou1, Bingjie Hu1, Tingsen Jing1, Zhe Li1, Fenglin Liu1, Hui Luo1, Qinglu Li1* and Hua Ye1*

...owth traits, correlation analysis between 31 SNP markers and growth traits in S. prenanti was analyzed using 164 samples with the same growth conditions. Principal component analysis showed that the body weight accounted for 94.50% of the variance, the eigenvalue was greater than 1, and the accumulative variance was more than 85%. So, the body weight was the first principal component of the growth traits for S. prenanti. Ass...

Mohsin Shad1, Muhammad Usman2 and Qurratulann Afza Gardner1*

...logenetic and mutational analysis. Polymorphism in the mitochondrial cyt b gene helps in studying the biodiversity and is a valuable tool for the identification of species. Mutations in the cyt b gene produce abnormal protein leading to deficiencies in the complex III (coenzyme Q), resulting in defective oxidative phosphorylation and consequently effects other metabolic pathways. In the present article, we comprehensively compare the biodiversity of cyt b in b...

Rukhsana Anwar1*, Ammara Abd-Us-Sattar1, Afifa Noor2, Kanwal Ashiq1,3 and Shah Jahan4 

...tive days. Real-time PCR analysis of hepatic CYP450 subtypes, CYP1A2, CYP2C9, and CYP3A4 was carried out. Moreover, phase II drug-metabolizing enzymes; GST-specific activity, total glutathione, superoxide dismutase, catalase, and malondialdehyde were determined in liver homogenates. The phytochemical analysis of extracts revealed the presence of many important bioactive compounds. Real-time PCR results showed that aqueous ex...

Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4

...ualitative phytochemical analysis showed the presence of active compounds. The antibacterial potential of various plant extracts was evaluated using well diffusion and disc diffusion assay against various disease-causing microorganisms. In the case of well diffusion assay, the plant exhibited a maximum 16mm inhibition zone against B. subtilis a minimum 10 mm inhibition zone against P. aeruginosa, with acetone extract. The same extract seems inactive against K....

Ahmad Abrar Khan1*, Ghulam Qadar1, Asif Ali Abro2 and Muhammad Awais3

...The result of regression analysis revealed that attack of insects/pests i.e. aphids, armyworm, mites, thrips and fruit fly were found non-significant while cutworm, whitefly and leaf miner have significant negative effects on per acre yield of tomato. Inappropriate and cultural practices, high dependency on agrovet dealers and fellow farmers were the key factors of yield losses. It is recommended to spray on the proper time along with follow-ups and physical c...

Malik Muhammad Yousaf1, Wali Muhammad1, Muhammad Mohsin Raza1*, Mumtaz Hussain1, Muhammad Jahangir Shah1, Bashir Ahamad1, Annum Sattar2, Hera Gull3, Sonia Sumreen4, Malik Waqar Yousaf5, Nazakat Nawaz6 and Nazim Hussain7

...CV). Principal component analysis showed that first three PCs reflected eigen values more than one where cumulative value was 61.78% of the total variability. The first one accounted as highest proportional value of total variability in all PCs and in remaining PCs lesser amount of variation. High values of heritability referred to broad sense genetic advance possessing higher values in plant attributes under study witnessed that these parameters abide by the ...

Malik Muhammad Yousaf1, Wali Muhammad1, Muhammad Mohsin Raza1*, Mumtaz Hussain1, Muhammad Jahangir Shah1, Bashir Ahamad1, Annum Sattar2, Hera Gull3, Sonia Sumreen4, Malik Waqar Yousaf5, Nazakat Nawaz6 and Nazim Hussain7

...CV). Principal component analysis showed that first three PCs reflected eigen values more than one where cumulative value was 61.78% of the total variability. The first one accounted as highest proportional value of total variability in all PCs and in remaining PCs lesser amount of variation. High values of heritability referred to broad sense genetic advance possessing higher values in plant attributes under study witnessed that these parameters abide by the ...
Aulia Evi Susanti1*, Rudy Priyanto2, Muladno2, Dewi Apri Astuti3, Lucia Cyrilla2
...lications. A descriptive analysis was conducted to evaluate the corporate model’s production system and business management. Production analysis was analyzed using ANOVA and data were processed using R studio software version 4.1.2. Furthermore, the observed parameters include average daily gain (ADG), dry matter intake (DMI), pregnancy rate, the ratio of male and female calf births, mortality rates, production costs a...

Eman S Ramadan, Mohamed E Ali, Mohamed A Elkhiat 

...raphy, serum biochemical analysis as well as rapid feline infectious peritonitis (FIP) test and Rivalta test for the diseased cats. On basis of these results, six cats diagnosed with feline infectious peritonitis (effusive form) involving liver injury. Serum microRNA-122 was estimated by real-time polymerase chain reaction in all cats. Effusive form of FIP with liver injury was manifested by abdominal distension with fluid, anorexia, icteric buccal mucous memb...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...to 50 wk of age. One-way analysis of variance (ANOVA) including a polynomial test was carried out to explore the linear and quadratic contrasts of increasing the BSFL levels on all the parameters. The results of this study showed a linear improvement (p < 0.05) in the egg production (by 3.38 percent points than control), egg weight (by 1.54 g than control), feed conversion (by 20% than control), and egg quality traits, such as Haugh unit, yolk color, shell ...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Mohammad A. Al-Mahaish4, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel5
...alyzed using the one-way analysis of variance (ANOVA) with a polynomial test to explore the linear and quadratic effects of increasing the MWM levels into the broiler diets. The results of this study showed a linear increase (p < 0.05) in the final body weight, body weight gain, carcass weight, dressing, and meat yellowness (b*) of broilers as the dietary MWM level increased. In addition, linear and quadratic increasing yields of liver, abdominal fats, and ...

Tilahun Bekele* and Abebaw H. Georgis

...dings of the statistical analysis exhibited that the majority of the response variables under consideration were significantly (P<0.01) influenced by seed rate, variety, and interaction of both factors except for days to maturity, plant height, and thousand seed weight. Based on the over-year combined analysis, the highest capsule number per plant (10.4), yield per plant (2.46), and total yield (703.9kg/ha) were noticed f...
Seyedmohammad Karimi1, Mohammad Darvishi2*, Mohammad Barati3, Ramin Hamidi Frahani4, Mohammad Hassan Kazemi5
...ent t-test were used for analysis of data. P < 0.05 was considered statistically significant. No significant difference was seen between case and control groups regarding age, gender, BMI and severity of the disease at the time of hospital admission (P > 0.05), which shows a random classification of two groups. The mean AST, ALT, ALP and CBC of patients in case group decreased compared to the control group but the difference was not statistically signifi...

Tariq H.Y. Al-Maliky*, Mahmood S. Hashim and Amal S. Al-Sheraa

...sults of the statistical analysis showed that the shrimp M. affinis (A) in salinity 3 was significant (P>0.05) between the average weights of all the average weights during all periods, while there were no significant differences (P<0.05) between the average weights of the 15 and 30-day periods. In treatment (B) 15, significant differences (P>0.05) were found between all periods of the experiment. Shrimp M. nipponense in salinity 3 (C) treatment, ther...

Hasina Basharat1, Muhammad Ramzan Ali2*, Aziz Ahmed2, Rehana Kausar2 and Shamim Akhter1

... based upon cost benefit analysis, feeding at 4% rate was considered best. The economic profitability for the culture of African catfish in earthen ponds showed that net profit is increased on increasing feeding level from 2 to 4% of wet fish body weight, however, upon further increase in feeding level from 6 and 8%, the feed cost was increased that decreased the net profit. So, it was concluded that the feeding level of 4% wet fish body weight was optimum for...

Sepri Reski*, Ridho Kurniawan Rusli, Montesqrit, Maria Endo Mahata

...data were analyzed using analysis of variance (ANOVA), and differences between treatments were tested using the Duncans Multiple Range Test (DMRT). The study’s results were that the administration of Turbinaria murayana seaweed fermentation products with different levels showed a very significant effect (P≤ 0.01) on the cholesterol content of egg yolks. However, no significant effect (P≥0.05) on egg weight, shell thickness, shell percentage, yolk p...

Salahud Din

...borders. The descriptive analysis indicates, no statistically significant (p>0.05) differences between the lateral and medial phalanges of all measured parameters. All the measured parameters for the digits of the forelimb were statistically significantly different (p>0.05) between male and female adult Chinkara except greatest Length (Lg) of the phalanx media of the forelimb. Similarly, significant differences (p>0.05) were present in all the studied...

Hidayat Ullah1, Sadia Tabassum1, Sultan Ayaz2, Shumaila Noreen1, Atta Ur Rehman1, Naveed Akhtar3, Muhsin Ali3, Zaib Ullah3* and Sajid Mahmood1,4

...and 16SrRNA phylogenetic analysis of Rhipicephalus haemaphysaloides and Rhipicephalus microplus showed similarity with already reported species in neighbor countries of study area. Number of tick specimens and infestation rate shows direct correlation with temperature but it shows fluctuation with humidity. Current study will be helpful in tick’s controlling strategies, goats and sheep breed selection and future research in this area.

...
Aadil Sultan, Mohsin Ahmad Khan*, Nadeem Ahmed, Muhammad Hassan, Rashid Bhatti, Hafsa Naeem, Muhammad Islam Khan, Saad Tahir,  Samia Afzal* and Ahmad Ali Shahid
...subsequent densitometric analysis was done using ImageJ software. The difference in protein expression of all three populations was analyzed by using one-way ANOVA. Clones with double and triple copies of genes showed an increased expression up to 3.8 and 3.1 folds as compare to the clones having single copy of gene. Gene multimerization strategy can be used to enhance production of recombinant proteins in bacterial expression system.

...

Ghada A. Ibrahim1*, Mohamed Sayed Helal1, Nahed Abd Elhafeeze Kamoura2, Mohamed Samir3,4, Amira Mohamed Mazid5, Mohamed F.M. Farag6

..., the hemato-biochemical analysis of 100 blood samples (50 from naturally infected and 50 from apparently healthy cattle) revealed significant decreases in the haemobiogram parameters levels meanwhile, leucogram picture total proteins, globulins, urea, creatinine levels were significantly increased in klebsiella-infected animals. An alarming increase in MDR and ESBL klebsiella bovine infections necessitates a controlled use of antibiotics in cattle farms and w...

Muhammad Shahid1*, Muhammad Idrees1, Afza Rasul2, Iram Amin1 and Samia Afzal1

...ion. Logistic regression analysis revealed that liver enzymes (ALT, AST, ALP, γGT, albumin), blood cell count (globulin, platelets, MCHC RBC, WBC, monocytes% and lymphocytes%), lipid profile (HDL, LDL, VLDL, TC) were significant predictive factors in HCV infection. Similarly, ROC analysis suggested that ALT, AST, ALP, MCHC, TC, and VLDL variables have the potential to discriminate HCV infection from healthy individuals...

Rana Aamir Shehzad1, Ghulam Sarwar1*, Sabir Hussain Shah2, Mukkram Ali Tahir1, Noor-us-Sabah1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1, Imran Shehzad1 and Usman Saleem4

...re and post-harvest soil analysis were performed. The growth and yield traits were recorded and data were analyzed using LSD at a 5% level of probability for significance differences. Results of the research intimated that highest height of plant (71.42 cm), shoot dry weight (10.74 g), length of spike (13.33 cm), tiller numbers in a plant (7.16), weight of dry root (0.335 g), root length (11.68 cm), weight of 1000 grains (37.88 g) and wheat biomass (48.63 g) w...

Songruo Tao1, Cuiyi Liao1, Jinju Peng1, Yuexia Ding1,* and Yi Ma1,2,*

...ibosomal DNA restriction analysis (ARDRA) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) method. The results showed that the number of Operational Taxonomic Units (OTUs) types decreased with the increase of florfenicol concentration, and the percentage of OTUs number to bacteria were the lowest at 100 mg·kg-1 florfenicol concentration after 21d treating, which was 8.33%. The phosphorus-solubilizing bacteria were...

Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5 

...s spectrometry (LCMS/MS) analysis was used to assess the confirmation profile protein of seminal plasma. The conception rate from artificial insemination was used to determine the fertility rate (AI). This investigation revealed that the quality of the sperms from Bali-polled bulls and Bali-horned bulls did not substantially change after thawing (p > 0.05). However, sperm acrosome integrity was considerably (p < 0.05) higher in Bali-polled bulls than in ...

Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1 

...n to Immunohistochemical analysis was done on the proliferating cell nuclear antigen (PCNA), hypoxia-inducible factor 1-alpha (HIF-1), PPAR, and cyclin D1. Results: Mice treated with Cis+Pio HD showed higher levels of SOD, GSH, p-ERK1/2 and p38-MAPK, as well as lower levels of MDA and tumor growth factor beside the higher apoptotic activity. Furthermore, Cis+Pio HD reduced the expression of PCNA and HIF-1 and increased the expression of PPARγ, which impr...

Imdad Hussain Soomro1, Gulfam Ali Mughal1, Naeem Rajput1, Rameez Raja Kaleri2,6*, Deepesh Kumar Bhuptani3, Raza Ali Mangi4, Ghulam Mustafa Solangi5, Sheva Dhari6, Abdul Wahid Solangi6 and Zoya Parveen Soomro

... weight gain statistical analysis showed that non-significant (P≥0.05) difference between groups from 1st to 4th week. However, showed significant (P≤0.05) effect was recorded from fifth week to eight week of age, respectively. Final weight was also significantly higher in B group when compared with A group. Statistical analysis showed non-significant (P≥0.05) difference between the groups from 1st, to 5th week. How...

Safriyanto Dako1, Nibras Karnain Laya1, Syukri I Gubali1, Ari Ardiantoro2, V.M Ani Nurgiartiningsih3, Gatot Ciptadi3, Desinta Wulandari3, Suyadi Suyadi3*

...or DNA extraction for an analysis fragment in the microsatellite DNA region using ILSTS017, HEL13, and BM1818 primers. A total of 74 alleles were identified across entire populations. The expected heterozygosity ranged from 0.407±0.216 (Bali) to 0.716±0.050 (Gorontalo), and the observed heterozygosity ranged from 0.471±0.084 (Bonebolango) to 0.778±0.222 (PO). F statistical analysis includes FIS 0....

Ghulam Jelani1, Muhammad Haris Raza Farhan2, Rida Asrar3, Muhammad Ahmad1,4*, Chanda Naseem5, Zafar Ali Khoso6, Majid Hussain Soomro7* 

...eview with a descriptive analysis to identify the chronic respiratory poultry disease, method of prevention, their seasonal incidences, and the treatment by the use of good antibiotics and supportive therapy.
 

...

Nasratullah Baloch1*, Naeem Tariq Narejo2, Hamida Narejo3, Muhammad Farooq Hassan4, Muhammad Hanif Chandio5, Faheem Saddar6, Dharti Shahnawaz Thebo7, Ghulam Abbas8 and Shahnaz Rashid8

...ber 2016. For month-wise analysis of zoo- and phytoplankton was performed by using net of 25 µm mesh size. For the qualitative and computable examination of planktons, the 50 liters of bay water was strained and residue fixed with 5% formalin in sampling bottles. The 99 genera of both the planktons were recorded, which included 52 zoo- and 47 for phytoplankton from the experimental area. Among the zooplankton, Copepods were found to be rich 65% followed ...

Shabana Noureen1, Shirmeen Ijaz2, Saad Ullah3, Iqra Shafique Abbasi4 and Shakeel Ahmad5*

...was used to do a content analysis of tweets. The content analysis reflected three major symptoms of social anxiety including behavioural, cognitive, and physiological. The tweets have discussed different domains that were coded into four major themes (i) Complications linked with social anxiety, (ii) Desire to overcome social anxiety, (iii) Disclosure of social anxiety in tweets, and (iv) Supportive tweets about social anxie...
 
 SADAF SARFRAZ1*, SAFDAR AMEER1, REENA AMBREEN1, SHOMAILA SIKANDAR 2, SHAISTA NAWAZ3 AND YASIR SALEEM
 
 ASMAT MEHMOOD, ZAEEMA KHAN AND NAILA MALKANI* 
...ss were determined. Data analysis indicated that oral cancer prevalence in Pakistan is increasing year after year, with the growth being particularly pronounced in the country's male population. From 2010 to 2016, the overall prevalence was 0.1 percent. The most common age of diagnosis for oral cancer was 45-65 years, and the standardized cancer incidence rate was 633.411 i.e. 8/1000000 with a confidence interval of 95%. The study concluded that oral cancer is...

SAIMA SHARIF*1, SHAGUFTA NAZ1, RIDA IRAM1, FARKHANDA MANZOOR1, TASNIM FARASAT1 and MUHAMMAD SAQIB2

...pecifically. Statistical analysis was done by using SPSS (version 13). Among the 500 subjects, according to haemoglobin level 130 subjects (26%) were normal whereas 370 (74%) were anaemic. It was found that 120 (24%) were mild anaemic, 151 subjects (30%) were moderate anaemic, and 99 subjects (20%) were severely anaemic. It has been concluded that the prevalence of anaemia was considerably high in children below the age of 12 years living in rural communities....
Jameel Ahmed1,2*, Mohammad Masood Tariq1, Nadeem Rashid1, Asim Faraz3*, 
Majed Rafeeq1, Irfan Shehzad Sheikh1, Mudassar Jahan1,2, Abdul Fatih1,2
Masroor Ahmad Bajwa1, Ifrah Jameel1, Muhammad Ali1 and Saira Iftikhar4
...ere subjected to genetic analysis using ovine molecular markers where fifteen sheep SSR genetic markers were employed for describing multiple-allelomorphism. The average observed number of alleles (Na), effective number of alleles (Ne) and Shannon’s Information Index were 2.07 ± 0.79, 1.86 ± 0.69 and 0.54 ± 0.29, respectively. Heterozygosity was described by the average, observed and expected heterozygosities whose values were found ...
Jian-Cheng Zhai1,2, Sheng-Nan Wang1,3, Qiang-Hui Wang1, Yan-Ling Xia1, Wei-Shi Liu1, Ya-Jie Yin4 and He-Ping Li1*
... Based on bioinformatics analysis, the regions of IGF1 (-50bp ~ +615bp), KGF (-285bp ~ +207bp) and NGF (-388bp ~ +109bp) were selected for studying the methylation status. The antler mesenchyme of male reindeer was the only methylation sample in IGF1 (2.23 ± 0.92%), significantly higher in methylation level than that of female reindeer and sika deer (0.01<P<0.05). Methylation levels of KGF in antler mesenchyme were highly significantly higher in m...

Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3

...c activity. Colorimetric analysis was done to determine the vitality of HepG 2 cells for 24 h after treatment with methanolic seed extract and minimum inhibitory concentration was calculated. HepG2 cell were transfected stably to express nonstructural proteins NS3 and NS5A of HCV 3a genotype. Western blot and RT PCR confirmed stable protein expression. Immunofluorescence assay was done to check subcellular localization of these nonstructural proteins. The CC50...

Amber Khalid1*, Amjad Rashid Kayani1, Muhammad Sajid Nadeem1, Muhammad Mushtaq1, Mirza Azhar Beg1 and Surrya Khanam2

... information on the diet analysis of Indian gerbil through stomach contents. Samples were collected from different localities of Pothwar during different seasons. Stomachs were separated during autopsy. Slides from stomach contents were prepared and were compared with reference slides. Relative frequency of every food item observed in the stomach was calculated as percentages. The results showed that Indian gerbil predominately consumed plant matter in its die...
Syeda Fakhra Waheed1, Asim Aslam1*, Muti-ur-Rehman Khan1, Kamran Ashraf2 and Beenish Zahid3
... followed by phylogentic analysis. A total of 4 isolates were sequenced based on partial VP1 and hyper-variable region of the VP2 genes simultaneously. According to phylogenetic analysis, the study isolates genotype A3B3 were identified as predominant strains in country backyard poultry. Concerning the identity matrix analysis of VP1 representative part, study isolates shared (87-88% nt; 9...

Zhe Lin1, Yumo Li1, Yuchen Wang1, Yuechen Li1, Ke Pei1, Guangfu Lv2, He Lin1* and Zhun Yu3*

...gets. Pathway enrichment analysis showed that there were 20 critical pathways. The results revealed that OR could increase the total swimming time, increase the survival time of enduring anoxia, and regulate monoamine neurotransmitters such as 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), norepinephrine (NE) and dopamine (DA). Western blot analyses indicated that OR may decrease the phosphorylation of p38. In conclusion, the results revealed...

Yang Hu, Jingxin Ding, Beilei Xu, Xiangming Sun, Guoyu Li, Hui Song, Zheng Zong and Wenlan Li*

...blished by UPLC-Q-TOF-MS analysis. The estrogen-like activity of TGs was evaluated by determining the proliferation rate of Michigan Cancer Foundation-7 cells. The spectrum-effect relationship was analyzed by serum fingerprint of TGs to characterize the activity in vivo and identify the active compounds of TGs. In the results, a total of 75 chemical compounds of TGs were identified in serum, including 11 parent molecules and 64 metabolites. The serum fingerpri...

Muhammad Shuaib1*, Abdul Hafeez1, Ziaul Islam2, Assar Ali Shah3 and Saad Ullah1

...layer on, feed proximate analysis, egg quality, and economies during peak egg production period with different phases (phase-1=week 29 to 32, phase-2=week 33 to 36, and phase-3=week 37 to 40). One hundred and sixty golden misri (Brown) laying hens of age 28 weeks were sourced and reared for 40 weeks. There were 4 groups with 4 replicates per group containing 10 birds each. Corn-soybean meal diet (Basal Diet) was offered to the control group (A), while groups B...

Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi 

Riko Noviadi, Dwi Desmiyeni Putri, Gusma Gama Maradon*, Agung Adi Candra, Nani Irwani, Gadis Apriani, Made Guntur Candra Adinata, I Made Krisnanda

...nd has economic value by analysis results price BEP of Rp. 25,645.35, Product BEP of 21.38 kg, IOFC of Rp. 734,032.90, R/C value of 1.44, and B/C value of 0.44.
 
Keywords | Economic, Maggot, Metabolic, Processing, Productivity
...

Yusmarini Yusuf*, Usman Pato*, Shanti Fitriani, Emma Riftyan, Evy Rossi, Diky Arma Fauzi, Ghina Ismadiah, Miftahul Hidayah, Windy Sabiliani

...yzed statistically using analysis of variance, and differences between treatments were analyzed using DNMRT at the 5% level. The results showed that the application of various preservatives might impact the quality of fishballs during the first 30 days of frozen storage. Even though the chemical and sensory parameters of fish meatballs without preservatives that were kept at freezing temperatures showed the count of microorganisms that had exceeded the quality...

Doungnapa Promket1,2,4*, Khanitta Pengmeesri2,4, Jennarong Kammongkun3, Thassawan Somchan2

...espectively. Statistical analysis results discovered significant associations between the three candidate genes (NPY, DRD2 and VIP). Egg production 270EN, 360EN and E_M of BB genotype were higher than bb genotype for the NPY gene (P < 0.01), while CC and TC genotypes of the DRD2 gene were associated with high 270EN, 360EN and E_M (P < 0.01). The DD genotype had higher 270EN, 360EN and E_M compared to ID and II genotypes, whereas other egg production trai...

Omnia M. Khattab1, Morcos I. Yanni2, Hala K. Abdelmegeed2, Mahmoud Eliwa1, Naglaa M. Hagag1, Sara M. Elnomrosy1

...nced Partially. Sequence analysis results showed no variation in their sequence across all isolates when compared to the published FPV genome, which could imply that FPV appears to be in genomic stasis as compared to other Parvoviruses. Genomic stasis appears among the three Egyptian samples in this study under the GenBank accession number OP594200, OP574201, and OP574202, in addition to the high similarity of the isolates the vaccine strains of EU498680 and E...

Faradji Khalil1*, Slimani Noureddine2 and Senoussi Abdelhakim3

...y methods and the use of analysis means adapted to the situations encountered. In this context, the means used to carry out this work are based on documentary research, surveys and observations. Based on field investigations, three main categories of livestock breeders were distinguished the farmers surveyed have at least an education level thanks to the reforms carried out by the Algerian state in the field of rural development and that this profession did no...

Siti Azizah1*, Salsa I Latifah1, Irfan H Djunaidi1, Anif M Wati1, Achadiah Rachmawati1, Siti Hamidah2 

... descriptive qualitative analysis. The results show that wives are productive in households, including on-farm, off-farm, and non-farm sectors. The contribution of wives who perform productive roles is essential. It is at 29.06%, with an outpouring labor of as much as 78,128 man-days/year and a productivity level of 112,515 IDR per man-day.
 

...

Abubakar Sadiq Muhammad1,2, Latiffah Hassan1, Saleha Abdul Aziz1, Zunita Zakaria1, Hassan Ismail Musa1, 2*, Maswati Mat Amin3 

...mial logistic regression analysis found a significant association between soil water content and ST (sequence type) 84 (OR = 0.833, 95% CI: 0.708 to 0.980; p=0.027) when compared to ST51. This showed a unit increase in soil water contents was associated with a 1.2 times increase in the odds of recovering ST51 compared with the odds of recovering ST84. Also, a statistically significant protective association was recorded between water pH and ST84 (OR=0.401, 95%...

Muhammad Ahsan Riaz1, Ayesha Riaz2, Muhammad Asif Mahmood3, Muhammad Uzair Mukhtar3*, Zaib Un Nisa1, Beenish Ijaz4 and Muhammad Shahid Rasool5

...y Faisalabad for further analysis. The larval morphological measurements were carried out using a stereomicroscope, which included changes in head length and width, thoracic length and width, abdominal length and width, and total length of the larva. Every month, the fourth instar larvae (n=36) were investigated for body size measurement. The results showed that low temperatures of breeding water significantly increase (P≤0.05) the body size, head, thorax a...

Ahmad Jupri1*, Yuliana Vofi2, Faturrahman2, Immy Suci Rohyani1, Ernawati1, Bulkaini3, Djoko Kisworo3, Wardatul Jannah4 

...oups. Therefore, further analysis is needed to determine the content of other bacteria in the water at the Joben spring, Pesanggrahan Montong Gading, East Lombok. 

...
M. A. Abou El- Nasr1, Kh. A. Dougdoug1, Hayam S. Abdelkader2, and Rehab A.
Dawoud2
...onfirmed by western blot analysis.

...

M. A. Amer1; M. H. El-Hammady2; H. M. Mazyad1; A. A. Shalabyl and F. M. Abo-El-abbas

...sted by PCR, restriction analysis, and translation. Analysis by in vitro translation using western blotting assay on nitrocellulose membrane using monoclonal antibodies verified that the PVY-CP gene correctly encoded and expressed a protein reacting with PVY antibodies. The CP gene, approximately 32 KDa reacted successfully with P VY specific monoclonal antibodies in dot blot immunobinding assay (DIBA) as well as in western ...

Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2

...and electron microscopic analysis confirmed the production of mature infectious virus particles underlining the conclusion that a full infection cycle was completed in this system. Both the structural viral proteins nucleoprotein (N) and the envelope glycoproteins GI and G2 and the nonstructural viral proteins NSs and NSm were accumulated to amounts sufficient for detection and cytopathological analysis. Electron micrograph ...

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

...lowed by electrophoretic analysis of the extracts on 5% polyacrylamide gel. A reverse transcription-polymerase Chain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA product of approximately 360 bp was successfully hybridized with digoxigenin labeled PSTVd cDNA probe.

...

Sahar. A. Shoman1 and B. A. Othman2

...ftware program. Sequence analysis of the amplified fragment revealed a high conserved sequence homology located at the 3'-terminal region of the 126KDa (ORF1) sequence of the published TMV strains.  Detection of the TMV-E using RT-PCR raises the availability of species-specific primers for the TMV-E that will be helpful for diagnosis in mixed infection. In addition, it will be helpful in further molecular characterization of this strain.

...

Yun Zhang*

...sion electron microscope analysis revealed that exosomes could be significantly observed in the PE group. The expression of surface markers that included CD63 and CD81 were dramatically upregulated in PE group. The protein level of ET-1 and Ang-II were significantly increased in PE group, and the upregulated ET-1 and Ang-II expression were highly correlated with PE progression. The present study demonstrated that the exosome protein ET-1 upregulated the expres...

Xiaoying Liang1,2, Xiaofang Jiang3, Honglin Jia1, Ru Zhang1, Li Gao4* and Qing Yang5*

...n CD4+ T cells. However, analysis of regulatory T cells revealed the opposite results. The decrease of Th2 cells, cytokine proteins, and genes was reversed by a DNA methyltransferase inhibitor in vitro and in vivo, which also increased the allergen sensitisation status and decreased the inflammatory response. Epigenetics has an impact on disease occurrence. Epigenetic regulation in T cells contributes to the development of experimental asthma, which can be tre...
A.l. Abd El-Fattah; A.s. Saclik M.M. El-Kholi; I.A. Åbdcl-Hmnid and M.A. Madkour
... obtained after SDS-PAGE analysis of the purified virus preparation. The SCMV-E-RNA was isolated from virus-infected sugarcane tissues and then used as a template for reverse used transcription-polymerase to amplify the coat protein chain reaction (CP) gene (RT-PCR) Of SCMV-E to amplify strain. the ThecDNA that directly amplified cp gene was used as a template using the internal primer combination in PCR for confirmat...
Zhao Fulin1,2, Liang Chen2, Tao Lin1, Jiang Yanting3, Ouyang Yina3, Hong Qionghua3* and Chu Mingxing1*
...P> 0.05). Association analysis indicated that there were no significant correlation between FGFR1 gene g. 12120297A> G locus and EPB41L5 gene g. 64266715G> C locus and litter size, litter weight at birth and weaning (P> 0.05). There were significant correlation between EPB41L5 gene g. 64237881A> C and g. 64266710 G> C and litter size (P< 0.05), and were no significant correlation and litter weight at birth (P> 0.05), there were no signi...

Wuritunashun*, Burenbatu, Narenqiqige, Jiuguniang, Eerdunduleng, Shuanglian Wang, Cuiqin Gong, Hashengaowa, Huizhi Jin, Baiwurihan and Chunhaizi

...egulated. Bioinformatics analysis of these differentially expressed proteins revealed that they play a critical role in immune-related responses. Parallel reaction monitoring validation also substantiated the results of the LFQ proteomics approach by analyzing the expression of LBP and SERPINA10. This work identifies the proteins and protein-related molecular pathways that are responsible for the efficacy of Zhenbao pill in the management of HSP.

...

Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1  

...ion, immunohistochemical analysis of CYP3A4, MRP-1, and c-MYC proteins and expression of miRNAs were assayed. Results: Findings indicated that CSO treatment led to a suppression in hepatotoxicity, nephrotoxicity and cardiotoxicity induced by DOX in male albino rats evidenced by a significant enhancement in the activity of ALT, GGT, LDH, AST, also creatinine and uric acid. CSO induced antioxidant activity GSH, CAT and inhibited MDA. Histopathological exami...

Adel B. Salama and Reham M. Sabry*

...ercentage and fatty acid analysis of pot marigold (Calendula officinalis). A Randomized Complete Block Design (RCBD) was used to arrange the treatments in three replicates, (H): petal harvesting and (C): without petal harvesting (control). Petal harvested plants produced a significantly much more harvestable total flower yield of 297-350 per plant than control with 48-51 flower/plant. Further, petal harvesting has significant effect on the flowers number and i...

Tajul Islam Mamun, Jillur Rahman, Mahmudul Hasan, Md. Jamal Hossain, Md Wakil Mahmud and Kamruz Zaman

...me provides an empirical analysis of FIP in the city of Dhaka. The results demonstrate an unambiguous correlation between age and neutering status. This finding will contribute to develop future investigations.

...

Thobela Louis Tyasi1* and Widya Pintaka Bayu Putra2

...nd PCA were utilized for analysis of data. The correlation results revealed that chest girth had a positively high statistically significant association (P < 0.01) with rump height ( r = 0.74) and withers height (r = 0.57), and a positive statistically significant association (P < 0.05) with face length (r = 0.40), body length (r = 0.47) and sternum height (r = 0.48). Three principal components (PC’s) were obtained in Nguni cows that explain about ...
Widya Pintaka Bayu Putra1, Herman W Raadsma2, Indriawati1, Slamet Diah Volkandari1 and Endang Tri Margawati1*
...orrelation model through analysis of variance (ANOVA) method. A total of 650 records of LS were used in this study, representing in the progeny of fourteen (14) rams collected from year 1999 to 2004 at Cimanglid Research Park, Bogor, Indonesia. This study showed that the average of LS in sheep samples was 1.34±0.51 with h2 value of 0.04±0.06 (low). However, six Merino rams (43%) had positive of EBV value. Moreover, the data records of LS from eac...

Yong Bin Zhao1, Hui Song1, Guo Gong Yi1*, Xi Lian Hao1, Bai Wang2, Zhi Wen Chen2

...justify;">Stable isotope analysis of feathers is widely used in bird ecology. Generally, studied birds should be captured to collect feather samples in a way that clarifies the attribution of feather samples. As some endangered birds cannot be captured, feather sampling is the only feasible method for isotopic research. However, whether it can be used for research on individuals remains to be clarified. In this study, the values of δ13C and δ15N of...

Hassan Raza1, Muhammad Kashif Afzal2, Muhammad Luqman1*, Tahir Munir Butt3, Muhammad Yaseen1 and Muhammad Umer Mehmood1

...ware were considered for analysis. According to the results, about 51% of the respondents in the study belonged to old age (above 45) and about 53.2% of the respondents were having matric education. Furthermore, results from correlation analysis showed that education, the number of olive trees and motivation to grow olive trees have a highly positive significant relationship with the level of awareness about olive cultivatio...

Muhammad Kefayatullah* and Said Wahab

...ity during the period of analysis. The data regarding various parameters were recorded at each 7days intervalup to 28 days of storage. The samples T1 (control), SA1 (1 % sodium alginate), SA2(2 % sodium alginate), MC1(1 % methyl cellulose), MC2(2 % methyl cellulose), P1(1 % pectin), P2(2 % pectin), BW1(1 % bees wax), BW2(2 % bees wax), CC1(1 % calcium chloride) and CC2 (2 % calcium chloride) were studied for TSS, pH, acidity, ascorbic acid, reducing sugar, non...

Abid Hussain1*, Hassnain Shah1, Muhammad Nadeem Iqbal2 and Tariq Sultan3

...ermined through marginal analysis. Unfortunately, Pak-seeder technology could not be demonstrated effectively due to few institutional and technical limitations. Thus, technical issues in its manufacturing as well as its use are highlighted. The study is based on the outcomes of six demonstration sites of micronutrients’ use for citrus and thirteen sites for vegetables’ production in tunnels viz. chili peppers, bell peppers, cucumber and bottle gou...

Asmaa A. M. Abdel-Ghany1, Ahmed N. El Taweel2, Yassmin Moatasim2, Nagwa S. Ata1, Amany Adel3, Ayman Hany El-Deeb4, Ahmed Kandeil2, Mohamed A. Ali2*, Hussein Ali Hussein4* 

...e sequencing and genetic analysis. Although both of H5N8 viruses were closely related to clade 2.3.4.4b, they were genetically distinct and had genetically different constellation forms. This study highlights the need of active surveillance of AIVs in backyard production sector for assessing the epidemiology, evolution, and diversity of currently circulating viruses in Egypt.
 

...

Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi 

...cificity using molecular analysis and sequences. A total of 100 fecal samples were collected from quails in various parts of Baghdad/ Iraq from January to September, and they were then tested for the presence of Cryptosporidium spp. using molecular methods (PCR), followed by DNA sequencing. The overall infection rate was 37% (37/100) of the samples were Cryptosporidium-positive by using PCR. Three of five quails whose 18S gene was amplified by nested PCR and s...

Roy Malindo1, Anuraga Jayanegara2, Osfar Sjofjan3, Siti Chuzaemi3*

...scriptive statistics and analysis of variance (ANOVA) using general linear model (GLM) was carried out using SAS OnDemand for Academics (ODA, Cary, NC, USA). The results indicated that the average dry matter supply was 49.6% of the cattle requirements, with 21.8% derived from forage and 27.8% from concentrate. The average daily gain was 0.4 kg/head/d, with males gaining 0.4 kg/head/d and females 0.3 kg/head/d. Based on age, the average daily gain of calves, yo...

Sukandi Sukandi1,4, Djoni Prawira Rahardja2*, Herry Sonjaya2, Hasbi Hasbi2, Sudirman Baco2, Sri Gustina3, Kirana Dara Dinanti Adiputra1

...analyzed using factorial analysis to compare Bali cattle (horned and polled) and the time factor of measurement (morning and afternoon), followed by Duncan’s test. The results showed that horned and polled Bali cattle were significantly (P<0.05) different in rectal temperature, erythrocytic counts, hemoglobin levels, and hematocrit values but not in skin surface temperature, respiration rate, pulse rate, and leukocyte counts. The time factor of measur...

Edwin Ormachea V1,2*, Bilo Calsin C1,2, Eyner Aguilar S3, Buenaventura Ormachea V4, Henry Gonzales C1, Yecenia M. Masias G5

...udy was to determine the analysis of principal components for morphological characteristics in Creole sheep. A population of 380 sheep classified by age was used and body weight data and the following morphological measurements were recorded: Head length; Head width; Head depth; Ear length; Ear width; Neck length; Neck perimeter; Height at the withers; Longitudinal body diameter; Thoracic perimeter; Bicostal diameter; Sternal dorsal diameter; Rump width; Rump ...

Bambang Hartoyo1*, Tri Rachmanto Prihambodo1,2, Wahyuningsih3, Sri Rahayu1, Fransisca Maria Suhartati1, Muhamad Bata1, Efka Aris Rimbawanto1

...xt-align: justify;">Meta-analysis of lactobacillus was built to evaluate the performance, histology and microbiota of lactobacillus on digestive tract of broilers. A database was built from previously published article from internet reporting lactobacillus as feed additives in broilers. Articles were strictly selected according to evaluation of title, abstract and parameter which used in the study. Database collected was statistically analyzed using the mixed ...

Abdul Hassan1*, Arshad Farooq1, Muhammad Ishaq2, Ghulam Sadiq2 and Asif Nawaz3

...sing principal component analysis approach and regression analysis. The data were collected randomly from 130 sampled vegetable growers comprised of 65 respondents each from Nowshera and Charsadda districts through pre-tested interview schedule during financial year 2020-21. Principal Component Analysis was used for determining the risk and coping strategies of the farmers. Moreover, Ordin...

Munawar Lal1*, Naeem Tariq Narejo2, Muhammad Hanif Chandio1, Faheem Saddar3, Hameeda Narejo4, Ghulam Dastagir5, Ghulam Abbas6 and Shahnaz Rashid6

...0.98). The length-weight analysis values and the coefficient of condition showed that the growth of the five dominant species from Nurri Lake, District Badin, Sindh, Pakistan, was nearly ideal and satisfactory. The relative condition factor (Kn) was found to be fluctuating between 0.98 and 1.01 in all five dominant species. Results indicated that the values of length-weight and coefficient of condition analysis were ideal an...

Nawfal Hammadi Jasim1, Yasmeen Jassim Mohammed2 , Jala Amir Salman Alahmed1, Majdy Faisal Majeed3* 

...hemical and histological analysis . Results: Comparison to the Control Group, the ratio of ALT in the blood serum significantly increased in groups 1 and 2 of MPH-treated rats, meanwhile, the ratio of AST remained unchanged significantly in Group 1. In the liver, MDA and GSH-PX rates were significantly increased (P≤0.05) in 1 and 2 groups compared to the 3 groups (the control), while SOD level significantly rises only in Group 2, also did not appear MPO v...

Muhammad Mukhtar1*, Lely Ummi Wakhidah2,Mohammad Zubair Hippy3 

...of beef cattle. The data analysis techniques used are Location Question (LQ) analysis, LQ Shift Share, Analytic Hierarchy Process (AHP) and SWOT (Strength, Weakness, Opportunities, Threat) analysis. The result of this study indicate that beef cattle are livestock that have regional advantages (base sector) and are a progressive sector in the development of beef cattle in Gorontalo because ...

Endang Baliarti1*, Muh Auliya Rozzaq2, Adi Tiya Warman2, Sigit Bintara3, Tri Satya Mastuti Widi1, Diah Tri Widayati3, Bayu Andri Atmoko4, Tristianto Nugroho1 

...s. Data were analyzed by analysis of variance followed by Duncan’s Multiple Range Test, Pearson’s correlation, principal component analysis (PCA), canonical discriminant analysis (CDA), and canonical correlation analysis (CCA). Results showed that Bali Cross (Limbal and Simbal) have higher body morphometric (P<0.05) but lower reproductive ...

Javeria Khourshid*, Ghulam Abbas, Shahnaz Rashid, Asma Fatima* and Abdul Malik

...riod. Comparative growth analysis of both fish species revealed that OxyAqua organic is highly suitable for enhancing the biological performance of fish in captivity. This study is recommended for intensive and semi-intensive fish cultivation technology development, water quality improvement and fish health management in aquaculture.

...

Budi Guntoro1*, Agung Triatmojo1, Bambang Ariyadi2, Nguyen Hoang Qui1 

...thrax and FMD. The risks analysis was performed by the great knowledge of farmers (65.7% and 77.9% respondents) to Anthrax and FMD and the dissemination of knowledge for FMD as well as a positive attitude for both Anthrax and FMD. However, the knowledge of Anthrax dissemination was risk (65.01% farmers had inappropriate practices). It could be concluded that Yogyakarta’s cattle farmers are susceptible pose a risk from FMD prevention practices. Besides, f...

Winda Sartika1*, Budi Hartono2, Hari Dwi Utami2, Lilik Eka Radiati2 

...itative descriptive data analysis. The results of the study show that the resilience of the beef rendang industry during and after the Covid-19 pandemic caused good collaboration and cooperation between the institution chain involved; supply begins with the provision of beef raw materials in terms of information flow, financial flow, and product flow. Vertical collaboration from rendang raw material suppliers strengthens the supply chain network to distribute ...

Hafiz Muhammad Walayat Ali1, Hafiz Basheer Ahmad1*, Zaheer Ahmad Nazar2, Muhammad Akhlaq Mudassir1, Mahmood-Ul-Hasan1, Abdul Khaliq1 and Muhammad Shahzad Afzal1

...RCBD during 2017-18. The analysis of variance revealed significant variations in cane weight, sugar recovery and smut incidence for both years. During the evaluation 84 and 80 clones were found resistant to smut in plant and ratoon crop respectively. Principal component analysis showed that first eigenvalue was equal to (1.78) and represented that (35.6%) of the total variation. The 2nd eigenvalue (1.077) and represented of ...
Muhammad Zahid Rashid1, Amina1*, Hafiz Wasif Javaad2, Muhammad Asim Rashid3 and Amina Rashid3
...ically assessed by using analysis of variance and means of treatments were compared by employing LSD test. Twenty different date palm cultivar seeds were put in sterilized soil. According to the outcomes, the Amber variety of date palm performed better than other date palm varieties in terms of many physical characteristics, including highest survival rate (100%) and plant height (126.5 cm), stem girth (38.3 cm) and leaf area (116.1 cm2). Another variety, Mabr...

Abdulaziz A. Alaqil

...e analyzed using one-way analysis of variance and multiple hoc Duncan’s test. The findings of the current investigation displayed a significant (p < 0.05) reduction in the productive performance traits, including the total feed intake, final body weight (FBW), BW gain, and feed conversion ratio in the EC group by approximately 7.7, 22.5, 30.6, and 33.3%, respectively, relative to the group C. The spleen, thymus, and bursa indexes as relative weights o...

Thekra Fadel Saleh, Omar Younis Altaey*

...stains for histochemical analysis. Dissecting microscope measurements and histological measures were taken, and immunohistochemical analysis was performed to evaluate B lymphocyte distribution. The study found that in Muscovy ducks, the left caecum was longer and heavier than the right caecum, and the proximal portion of both caeca was wider and had thicker walls compared to the middle and distal portions. Histologically, th...

Fakhra Nazir1*, Sahar Fazal1 and Fakhar-i-Abbas2

...ication and phylogenetic analysis through DNA barcodes using mitochondrial COI gene (cytochrome c oxidase subunit I) was aimed for samples of birds collected from different regions of Pakistan. Mitochondrial DNA was successfully extracted from keel tissue and Folmer region of COI gene comprising ~650 bps was amplified using universal primers and PCR products were confirmed by 1% agarose gel electrophoresis. Sequencing was carried out by Sanger’s method a...
Umara Amir ud Din1, Waqas Ahmed Khan1*, Khurrum Shahzad1,2, Basharat Ali3, Misbah Hussain1,4 and Fazli Rabbi Awan4
...y PCR assay. Statistical analysis showed that subjects with DN had higher (67%) frequency of ID genotypes and the ID genotype carriers also exhibited higher levels of creatinine (6.2±3.9 mg/dL) and urea (99±41 mg/dL). Moreover, logistic regression analysis also indicated that ACE ID genotype carriers had a 2.5-fold higher risk of DN as compared to the II genotype carriers. Hence the present study shows a strong...

Achmad Firman1*, Sondi Kuswaryan1, Lilis Nurlina1, Muhamad Hasan Hadiana1, Marina Sulistyati1, Unang Yunasaf1, Dwi Cipto Budinuryanto2, Iman Trisman3

...sults determine that the analysis of Key Performance Indicators (KPIs) for the SIWAB/SIKOMANDAN program shows that the performance category was good and excellent. The proves that the five aspects assessed by KPI’s analysis, namely frozen semen distribution, cow acceptors, cow pregnancy, S/C rate, and calf’s birth are in accordance with the targets set. Meanwhile, the economic impact arising from this program cou...

Zahra Abdulameer Al-Zayadi1, Hana Kadum Shanan1, Al Salihi Karima Akool2

...nd sent for histological analysis. No abnormal histological features were seen in liver and kidney sections from the control and other mice groups before various treatments. The various treatment groups revealed diverse histopathological changes compared to the control groups. Various histological features were also seen in low, moderate, and high-dose Sc-RE treatment groups, accompanied by regeneration related to the treatment dose. Severe damage comprising n...

Rameez Raja Kaleri1,2*, Hubdar Ali Kaleri1, Raza Ali Mangi3, Deepesh Kumar Bhuptan4, Sana Noor5, Zainab Lanjar6, Hubdar Ali Kolachi7 Abdul Wahid Solangi1 and Sheva Dari1

...he standard procedure of analysis of variance. The results showed that the average birth weight of both Rakhshani and Harnai breeds was 3.17 ± 0.61 and 2.91 ± 0.51, respectively. The average weaning weight was also higher for Rakhshani than Harnai, with 15.21 ± 2.43 and 14.37 ± 1.27, respectively. The average body weight at 6 months and 12 months was also higher for the Rakhshani breed. The results showed a statistically significant...

Lu Xie1,2,3,4,5, Ye Gu1,2,3,4, Qiang Liu1,2,3,4, Hongzhang Shen1,2,3,4, Yifeng Zhou1,2,3,4, Jiangfeng Yang1,2,3,4, Xiaofeng Zhang1,2,3,4* and Jinyu Huang1,5*

...l curves and correlation analysis by GEPIA supported the significant roles of SET7/9 and E2F1 in the progression of HCC. Functional enrichment analysis suggested that the SET7/9-E2F1 axis is involved in the regulation of cell cycle, DNA repair and replication, and gene transcription. Our results implicated the potential of SET7/9 in combination with E2F1 as novel therapeutic targets and prognostic biomarkers in hepatocellula...

Mati Ullah1*, Muhammad Rizwan2, Ali Raza3, Xianlin Zhao4, Yanling Sun4, Sarah Gul5, Muhammad Ihtesham Waheed6, Muhammad Nadeem Khan7, Alvina Gul8, Sami Ullah Jan9* and Chao Huang4*

... to 2888. The pan-genome analysis of the three strains identified a total of 5449 genes with 377 as core genes. The functional characterization by subsystem category distribution analysis revealed “Carbohydrates” is the largest subsystem followed by “protein metabolism” while the biological pathway analysis by the Kyoto encyclopedia of genes and genomes (KEGG) datab...

Ruobing Han, Shanshan Gui, Xunwu Zhao and Heping Li*

...ition, genetic structure analysis was carried out. The results showed that a total of 67 alleles were detected, and the average number of alleles at each locus was 6.7. There was high genetic diversity but severe inbreeding in the white-red deer population, and there is no father-mother-offspring relationship or father-offspring relationship in white-red deer population. Our study provides the essential data support and reference for the conservation and susta...

Aditya Chowdhury Avi1,4*, Md Tariqul Islam1,4, Md Saiful Bari2,4, Rebeka Sultana Swapna Khandoker3, Goutam Buddha Das1 

...s. Data for cost-benefit analysis revealed significantly increased net profit in FO-treated groups. However, abdominal fat increased linearly with increasing levels of FO supplementation. The serum HDL level was increased, whereas cholesterol, LDL, and triglyceride levels were significantly decreased with increasing levels of FO in the broiler diets. It was concluded that supplying 2% or 3% would be effective in both increasing market weight and improving the ...

Falah Mahmood Hameed1*, Asaad Khalaf Talal AL-Shuwaili2, Eman Jawad Jabber3, Mayada Sahib Hassan1  

...wounds, for histological analysis at the 0, 3, 7, and 14-day post-wound production and treatment durations. The findings showed that the speed of wound healing in treated rabbits were faster than non-treated rabbits. Additionally, the findings showed that treated groups had improved cellularity and expanded angiogenesis compared to non-treated group. Conclusion: The histological results supported the hypothesis that chitosan enhances and promotes the developme...

Khalid Mahmood1*, Arslan Lodhi1, Muhammad Asim2, Muhammad Adeeb Babar3 and Muhammad Akbar Khan1 

... Following morphological analysis, measurements, and comparison to previously described fossils, these specimens are assessed up to species level and identified as belonging to Gazella lydekkeri, Selenoportax lydekkeri, S. vexillarius, Tragoportax punjabicus, T. salmontanus, and Pachyportax latidens. The presence of these bovid species indicates moderate taxonomic diversity of bovid fauna in the Dhok Pathan region of the Siwaliks of Pakistan.

...

Amr N. El-Shahat1*, A.M. Abdul Azeem1 and Mohamed H.M. Abd el Megid2

...were identified by GC/MS analysis including 1.8-cineole, Linalool and sabinene which represent the main volatile oils of bay leaf. Supplementation of BLE with HCD for 10 weeks resulted in reduction in the levels of some lipid contents (total cholesterol, triglycerides, Low-density lipoprotein-cholesterol and very Low-density lipoprotein-cholesterol), tumor necrotic factor-alpha and interleukin-6 as well as reduced activity of hepatic and cardiac enzymes. Also,...

Yufang Liu1,2, Guiling Cao3, Yujing Xie3 and Mingxing Chu1*

...ing to the gene function analysis in KOG database, the known genes were clustered into 10 subdivisions in A group, 8 in E group and 9 in B group under three categories, mainly in posttranslational modification, protein turnover, chaperones, energy production and conversion, and transcription process. Pathway analysis in KEGG pathway database of the known genes revealed that the five pathways that most differentially expresse...

Ahmad Noor and Malik Muhammad Shafi*

...istic model was used for analysis. The study discovered lack of surface water resources and ground water extraction satisfied the community needs and rainfall was only recharge method. Boreholes pumping technique was used mostly for irrigation. The Logit model investigated the impact of climate change was responsible for 73 percent variation in groundwater level. These climate factors included precipitation (Rainfall), temperature, humidity and delayed seasons...

Fadzlin Afiqah A. Samad2, Amirul Faiz Mohd Azmi1, Muhamad Affan Ab Azid1, Hafizin Mu’izz Zalazilah1, Syakirah Zulkifli1, Izreen Edriana Mohd Jasmi1, Muhammad Baqir Irfani Rahimin Affandi1, Mohd Zamri Saad1, Md Zuki Abu Bakar1, Agung Irawan3,7, Adib Norma Respati4, Anuraga Jayanegara5,7, Sadarman Sadarman6,7, Hasliza Abu Hassim1,2,7*. 

...is study presents a meta-analysis of 20 independent studies to investigate the relationship between forage to concentrate (FC) ratio, nutrient constituent, and nutrient intake on milk production and milk component in dairy buffalo. A dataset comprised of 89 comparisons from multi-species of buffaloes were analyzed according to a linear mixed model methodology with explanatory variables declared as fixed effects and individual study as random effects. The resul...

Salvator Minani1,2*, Eric Nsengiyumva1, Anatole Bigirimana1,2, Arnaud Cubahiro1,2, Dieudonné Ntakirutimana1,2 and Vénuste Bizoza1,2

... bile ducts. Statistical analysis was done using R software. A total of 265 domestic ruminants including 69 cattle, 190 goats, and 6 sheep were inspected to determine the presence of Fasciola spp. in their livers. The prevalence of Fasciola spp. was 13.04% (95% CI: 5.10-20.99) in cattle, 3.16% (95% CI: 0.67-5.64) in goats and 0.00% in sheep. The difference between the prevalence of Fasciola spp. in cattle and goats was statistically significant (OR= 4.60 (95% ...

Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1* 

...alyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 10 µM, 30 µM and 50 µM genistein treatment in the basic diluent tris aminomethane–egg yolk. Progressive motility post-thawing at 1 hour and 2 hours was highest at genistein concentration 30 µM (P<0.05). The effect of the antioxidant genistein on tris aminomethane - egg yolk diluent gave a significant effect on sperm viability in the Before Free...

Dina A. Yousif, Mohamed M.T. Emara, Marwa R.S. Abdallah*  

... parameters, and sensory analysis. The use of both extracts improved the overall sensory attributes, eliminated the unfavorable mutton flavor, improved the fatty acid composition, and produced a more stable color with a higher tenderness score. It has been concluded that the aqueous extracts of Mentha piperitae folium and Zingiber officinale can be utilized to improve the quality of old-aged mutton and facilitate its use in further processed meat products with...

Antonius Agung Wiono1, Maria Elizabeth Nana Wijayanti1, Dhika Yonika Primacitra1, Trioso Purnawarman2, Andriyanto3, Aulia Andi Mustika3, Amaq Fadholly3* 

...matology and biochemical analysis. Results: PyhtoceeTM dose 200 g/ton of feed had higher final weight and body weight gain with lower FCR, increase the valuesof RBC, HTC, lymphocytes, HDL and lowest mortality ratesamong the groups. While, the triglycerides, cholesterol, LDL and cortisolvalues were lower compared to others group, also the data of SGPT and SGOT values were similar to normal control. Conclusion: The data showed that PhytoceeTM 200 g/ton of feed h...

Yanping Li, Fei Xu, Yongming Wang*, Yunyun Lv, Jinrong Shi, Biwen Xie, Wenyao Cai and Dian Liu

...on. Population structure analysis and UPGMA tree indicated S. superciliaris and S. superciliaris ♀ × S. reevesae ♂ were clustered together that comprised of clade I, and S. reevesae and S. superciliaris ♂ × S. reevesae ♀ were clustered together that comprised of clade II, suggesting the close genetic relationship between the offspring and their female parents. This study provides reasonable comparison of genetic diversity and potential appl...

Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*

...ted to the two-way ANOVA analysis to determine the significant differences between species/breeds. The acidity, density and fat in Talesh sheep’s milk was higher than milk from other species/breeds (P<0.01). Also, the amount of protein in Talesh sheep’s milk was significantly higher (P<0.05) than all animals tested with the exception of Talesh buffalo milk. The pH of Saanen goat’s milk was higher than the pH of milk from all other speci...

Muhammad Aijaz1, Nasir Mahmood2, Ghulam Mujtaba3 and Imran Riaz Malik1*

...ts (n=100) for molecular analysis of the human IL-10 gene mutation at the -1082 (G/A) position. Extraction of genomic DNA was done from patients and healthy subjects. An allele-specific polymerase chain reaction determined polymorphism at a position (-1082 G/A). PCR products were analyzed by agarose gel electrophoresis to detect polymorphism. Further analysis was done by sequencing the PCR products from some randomly selecte...

Siswanto Imam Santoso*, Agus Setiadi, Wahyu Dyah Prastiwi 

...al perspective, economic analysis, and environmental viability. 

...

Muhammad Salman Ikram1, Tahir Mehmood1,2*, Sehrish Firyal3, Huma Sattar4, Shagufta Saeed3, Fareeha Nadeem3 and Muhammad Imran Mahmood Khan5

...extensively used for DNA analysis of such cases with limited information of autosomal DNA profiling, difficult to obtain data or identification has to be made from Y-chromosome markers. This review describes the uniqueness of structure and relative position of Y-STRs loci, history of Y- chromosome, role of genetic markers like Y- Short tandem repeat (Y-STR) and Y- Single nucleotide polymorphism (Y-SNP), and reveal the importance of next generation sequence (NG...

Abdulaziz A. Alaqil*

...l procedure with one-way analysis of variance (GLM-ANOVA) was used to analyze the data. The results showed a significant (P<0.05) increase in the BT, H/L ratio, MDA, and CORT, and over-expression of HSP70 in the hens of LPS group. Also, significant (P<0.05) impairments were perceived in the feed consumption and conversion, and in the egg weight and production rates. In contrast, the elevated stress markers and HSP70 expression in the challenged bird...

Desi Kurniati Agustina1,3, Suyadi Suyadi2*, Veronica Margaretha Ani Nugiartiningsih2, Kuswati Kuswati2 

...roximately 4°C. Data analysis was conducted at the Veterinary Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia. A statistical analysis was conducted using analysis of variance using Proc Mixed with general linear model (GLM) using SAS studio for academics Online Edition. This study showed the Haematological profile and leucocyte d...

Altaf Ur Rahman1, Muhammad Ajmal Khan2*, Ali Hazrat3, Awais Farid4, Muhammad Yahya3* and Inam Ullah5

Abdur Rauf1*, Muhammad Jawad1, Farooq Jan1, Muhammad Qayash2, Muhammad Yasin4, Samrin Gul5, Wisal Khan3, Ikramullah Khan1, Farkhanda Bibi1, Wajid Khan6, Tanweer Kumar6, Muhammad Arif6 and Khilwat Afridi7

...-days interval. A pooled analysis across the same environment revealed a significant difference (P ≥ 0.01) for all the parameters i.e., days to heading, days interval, plant height, flag leaf area, tillers m-2, spike length, spikelet spike-1, grain spike-1, thousand grain weight, grain yield and biological yield across three planting dates i.e., early, normal, and late respectively. Differences between wheat genotypes and genotype-environment interaction we...

Abdur Rauf1*, Farooq Jan1, Muhammad Qayash2, Zia-ul-Islam3, Rizwanullah1, Ikramullah Khan1, Muhammad Shuaib4, Muhammad Khalid5 and Samrin Gul6

....01%). The phytochemical analysis revealed the presence of alkaloids, saponins, flavonoids, and phenols in the ethanolic crude extract.

...

Abdur Rauf1*, Muhammad Sadiq1, Farooq Jan1, Muhammad Qayash2, Wisal Khan3, Ikramullah Khan1, Khilwat Afridi4, Muhammad Shuaib5, Muhammad Khalid6 and Samrin Gul7

...d 1000-grain weight. The analysis of variance showed significant differences for all the mentioned parameters (P ≤ 0.05). In local germplasms, all parameters revealed significant results excluding the 1000-grain weight, while the exotic germplasms also showed significant genetic variation except spikelets per spike and 1000-grain weight. The local varieties took more time for days to heading (Paseena-17, 147.7 days), more spikelets per spike (Khaista-17, 24...
Umer Farooq1,2, Nimra Murtaza2, Abubakar Siddique1, Bilal Saleem1, Obaid Ur Rehman1, Nageen Zahra1, Muhammad Uzair1, Muhammad Naeem Riaz1,3* and Muhammad Ramzan Khan1*

Luh Gde Sri Surya Heryani1*, Ni Luh Eka Setiasih1, Ni Nyoman Werdi Susari1, I Made Merdana1, I Gusti Ngurah Bagus Tri Laksana2, I Wayan Nico Fajar Gunawan2 

Komal Javed1, Sohaib Muhammad1*, Zarghaam Khan1, Sehar Fatima1, Hassan Nawaz1, Mahrukh1, Tahira Khalid1 and Shariat Ullah2

...en for the synecological analysis of weeds of wheat, rice and sugarcane crops of Tehsil Muridke, District Sheikhupura, Pakistan. Sampling was done randomly with the help of a quadrat method (1m2). For each crop, sixty quadrats were taken from each village i.e., twenty quadrats from each selected site. The data was gathered after every fifteen days during the course of crop. For the determination of Importance Value Index (IVI) percentage frequency, density and...

Arshad Javaid1*, Iqra Haider Khan1, Malik F. H. Ferdosi2, Aneela Anwar3 and Mujahid Manzoor4 

...t was subjected to GC-MS analysis. Major compounds in the flower extract were germacrene D (12.44%), n-hexadecanoic acid (12.15%), and caryophyllene (11.28%). Moderately occurring compounds included docosane (7.68%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (6.92%), pentadecane (6.20%), cyclopentanone, dimethylhydrazone (5.93%), dotriacontane (5.91%), humulene (5.82%), octadecanoic acid (4.05%), cis-vaccenic acid (3.72%), heptadecane (3.38%), and hep...

Salman Ahmad1*, Muhammad Asim1, Muhammad Zeeshan Majeed2, Muhammad Atiq3, Yasir Ali4, Hafiz Bashir Ahmad5, Malik Abdul Rehman6 and Naeem Akhtar7

...rrelation and regression analysis were used to determine the relationship of ACP with CG, and the relationship of environmental factors with ACP. Surveys were conducted to record the incidence of CG and estimate the yield losses caused by it. The study of population dynamics of ACP revealed that ACP population was higher between 7th to 14th April and 11th to 18th September, during 2017 and 2018. While, there was less ACP population between February 10th to 2nd...

Zia Gul1, Muhammad Sajid1, Muhammad Noman Khan1*, Faheem ul Haq1, Zohra Nawaz1, Muhammad Bakhtiar2, Sajid Siddique1, Komal Aslam3 and Muhammad Irshad1

... three replications. The analysis of data showed that organic manure and irrigation intervals significantly affected most of the growth and yield parameter of Aloe vera plant. However, irrigation intervals exhibited the highest leaves (number) (9.9) plant-1, plant height (34.5 cm), leaf length (36.8cm), leaf area (173.5 cm2), leaf breadth (4.0 cm), leaf thickness (3.1 cm) leaf weight (181.3 g), number of suckers (5.5) plant-1, amount of gel (149.3 g) leaf-1, a...

Kazi Afsana Homayra Orchy1, Mst. Antora Akter1, Nelema Yesmin1, Md. Moshiur Rahman Khan1, Marzia Rahman2, Md. Mahmudul Alam1* 

...rmed in the histological analysis of biopsy sample. Regarding the liver and kidney functions, the serum levels of liver enzymes (ALT, AST and ALP) and kidney function biomarkers (creatinine, BUN) showed a significant increase in burn patients of the saline-treated control animals, whereas, the animals treated with hPRP exhibited the normal range of these functional biomarkers. On the basis of morphology, histopathology, and biochemical <...

Walaa I. Mohameden1, Haidy G. Abdel-Rahman2, Ibrahim M. Hegab3* 

...ological and biochemical analysis. Results revealed an overall improvement in animal health, skin conditions, and liver and kidney functions. Significant (P < 0.05) increases in plasma proteins, zinc, and copper were recorded, while malondialdehyde, TNF-α, and MMP-9 significantly (P < 0.05) decreased. Furthermore, buffalo calves showed significant ( P < 0.05) increases in both ingestive and locomotor activities, while grooming and lateral recumb...

Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*

... performed transcriptome analysis of cattle embryos based on a single Cell RNA-Seq. Bovine sex-controlled semen for artificial insemination were used to obtain different stage embryos: bovine 8 cell XX embryo, 8 cell XY embryo, 16 cell XX embryo, 16 cell XY embryo, morula XX embryo, morula XY embryo, blastocyst XX embryo, and blastocyst XY embryo. A sequencing library was constructed by the Smart-Seq2 amplification. The transcriptome was sequenced by Illumina ...

Yakun Ge

...nvironment. Quantitative analysis of ROS was performed using flow cytometry and median fluorescence intensity detection. Cell proliferation was detected by MTT method. Combined treatment promoted the production of ROS. The cell viability of the combined treatment group was lower than that of the control group at 24h, 48hs and 72h (P<0.05). Compared with the control group, the expression level of E-cadherin mRNA in the combined treatment group increased (P&l...

Amirul Faiz Mohd Azmi1,4,Wan Nur Hanani Wan Roslan1, Athirah Zawani Zulkifli Amin Rashid1, Siti Aisyah Mohd Hafiz Ngoo1, Mohd Hezmee Mohd Noor1, Muhammad Azrolharith Rashid1, Mohd Zamri Saad2, Danung Nur Adli5, Hasliza Abu Hassim1,3* 

..., pH, and meat proximate analysis. The results revealed that the body weight, average daily gain, dressed weight and pH value were significantly differ (p<0.05) in treated group 3 compared to the control without soybean waste. The carcass crude protein and fat were also significantly (p<0.05) better. In conclusion, adding soybean waste into quail diet for up to 30% could be a potential protein source that enhances the growth and improves meat quality of ...

Yao Xixi1, Zhou Rui2* and Ma Yinshan3

...ilk fat. The correlation analysis was studied in order to find out the influence of forage and rumen fluid parameters on the content of CLA in milk of grazing yak. The results showed that the phenological phases significantly affected the content of lauric acid, myristic acid, palmitic acid, stearic acid, linoleic acid, linolenic acid, total fatty acid, polyunsaturated fatty acids (PUFAs) and total volatile fatty acid (tvfa) (P<0.05), and these were signifi...

Bushra Ainy Dars1*, Naeem Tariq Narejo1, Muhammad Hanif Chandio2, Hamida Narejo3, Majida Parveen Narejo4, Faheem Saddar5, Ghulam Dastagir6, Ghulam Abbas7 and Shahnaz Rashid7

...easurements and meristic analysis that there was a single homogenous population of Clarias gariepinus, available in ponds. The culture of this species may produce a significant role in generating revenue in the country.

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Nadia Jabeen1, Abdul Mubeen Lodhi1*, Rehana Naz Syed1, Muhammad Ali Khanzada2 and Alia Gul3

...c Pakistan. Phylogenetic analysis of the obtained sequences was done based on the maximum similarity method using Mega 6.0. The spore was smaller as compared to Marasmiellus scandens (6.2-8.7 µm). The newly described species i.e., Marasmiellus agrianum N. Jabeen and M. Lodhi differs from other species based on the molecular study as well with few morphological characteristics; size, color, shape, and texture of pileus, and stipe, and the molecular charac...

Ertika Fitri Lisnanti1,4, Widya Paramita Lokapirnasari2*, Eka Pramyrtha Hestianah3, Mohammad Anam Al Arif2, Zein Ahmad Baihaqi4,5 

...Data were analyzed using analysis of variance (ANOVA), if the results were significant, continued with the LSD test. The value is considered significant if P < 0.05. Blood collection from the axillary vein of broiler chickens which is put in an EDTA tube. The parameters measured were: the number of leukocytes, erythrocytes, hemoglobin levels, hematocrit, platelets, lymphocytes, monocytes, granulocytes, types of leukocytes and blood glucose levels in broile...

Sagir Hussain1, Erum Iqbal2*, Nasira Kazi2, Sher Wali Khan1, Qamar Abbas1 and Abdul Razaq1

...Baltistan, Pakistan. The analysis of samples yielded a new nematode species and two new reported species belonging to the order Tylenchida as new geographical records for Pakistan. Hemicycliophora pyri n. sp., is characterized by the broadly rounded lip region with two indistinct annuli, closely fitting sheath with 231-247 body annuli, stylet 95-100 µm long, non-raised and non-separated labial disc and gradually tapering long tail. Psilenchus vincigurrae...

Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2* 

...sion: The study provides analysis for the 5EG-QH19 wild-type RABV genome, elucidates some key structural features of the AF4 lineage dominant in Egypt and supports the recently proposed division of AF4 lineage into two sub-lineages (AF4a and AF4b). Divergence from SAD B19 vaccine strain sequence was also reported but the exact effect of the observed genetic changes in an infectious RABV particle dictates in-vivo studies. 

...

Elihasridas, Mardiati Zain*, Roni Pazla, Simel Sowmen, Qurrata Aini 

... at 39ºC/48 h. The analysis of variance following a 5 x 4 randomized block design was used. The results revealed a significant (P<0.05) increase in the digestibility of ammoniated aromatic lemongrass waste in the rumen. The degradation of ammoniated aromatic lemongrass waste was found to be significantly (P<0.05) higher when supplemented with cassava leaves and mineral zinc (T3) compared to others. However, it was observed to be significantly (P<...

Jaisy Aghniarahim Putritamara1*, MB Hariyono1, Budi Hartono1, Hery Toiba2, Hamidah Nayati Utami3, Moh. Shadiqur Rahman2, Tina Sri Purwanti1

...ral Equation Model (SEM) analysis. Data were collected from a survey with 210 business people selected using a purposive method. The first inclusion criterion is running a micro, small, or medium enterprise (MSME) in the beekeeping industry (with business sizes determined based on the number of workers, assets, and turnover as stipulated in the Law of the Republic of Indonesia No. 20 of 2008 concerning MSMEs. The second criterion is having been in business for...
Tabark F Ahmed, Nibras Z. Yahya*
 
.... Furthermore, time-kill analysis showed that ASCORBIC/CIP combination exhibited highest synergistic and bactericidal effect. It was determined that in vitro study against E. coli O157 in the treatment of urinary tract infection, the combination of ascorbic acid and ciprofloxacin was both more potent and safer than the combination of ascorbic acid and erythromycin. From this study concluded the combination of ciprofloxacin, and erythromycin against isolated E....

Pham Tan Nha*, Le Thu Thuy

...d the FG treatments. The analysis of chicken manure microorganisms revealed that the G1.5 treatments exhibited the highest levels of Lactobacilli, while the G1.5 treatments demonstrated the most favorable results with regards to lower levels of E. coli and Clostridium. Overall, the results indicated that incorporating 1.5% (DM) Black Garlic into the diet of Tre chickens between 6 to 13 weeks of age produced the best outcomes.
 
Key...

Khalid M. Al-Syaad1,2

...ollected for biochemical analysis, and tissue samples (liver, kidney, and testes) were collected for histopathological examination. The results showed that the presence of CC increased (P<0.001) the levels of liver enzymes (ALT; alanine aminotransferase and AST; aspartate aminotransferase), creatinine and urea in the blood serum and lipid peroxidation product (TBARS). While decreased (P<0.001) the total antioxidant capacity (TAC) and number of spermatozo...

Lichun Jiang1,2*, Lan Zhu1, Meiqi Li1, Xinyue Bao1, Zhenkun Zhao2, Haifen Qin2 and Wei Chen3*

...g genes and phylogenetic analysis, Elaphodus may have a sister relationship with another deer group Muntiacus and they belong to the monophyletic genus. This study depicted mitochondrial genome characteristics for E. cephalophus and deepened the understanding of the phylogenetic relationship of Cervidae.

...

Ramanathan Baluchamy* and Gnanamani Radhakrishnan

...s were subjected for the analysis of biochemical compounds such as organic carbon, nitrogen, phosphorus, potassium and calcium. The site which is occupied by millipede (site I) showed remarkably high quantity of nitrogen (2.14%), phosphorus (23.10%), potassium (0.24%) and calcium (0.83%) than the site without millipedes (site II) during the month of November 2016. Similarly, an ideal C/N ratio (20.05) was observed in site I which favours the plant growth, in t...

Sumei Zeng and Yong Zhang*

...s were selected by theme analysis method based on the opinion of managers working in industries that are using IOT. The research method is applied in terms of purpose and is descriptive in terms of data collection tools. The results showed that Detection of Insemination Time (DIT) and Early Detection of Diseases (EDD) applications are more important, respectively.

...

J. Ma1, F.B. Zhang1,2, J. Li1, Z.Y. Lv1, C. Zhang1, L. Mao1 and Y. Zeng1*

...microscopic examination, analysis of similarity, and the Amundsen graphical method. A total of 26 food categories were identified, among which algae and organic detritus were the dominant food source for S. dabryi. Animal prey contributed relatively low proportions to the food of S. dabryi. Seasonal variations of food composition of S. dabryi were significant (P<0.05). Hierarchical cluster analysis shows that the food com...

Kiran Afshan1*, Sobia Baseer1, Shanza Kiran1, Ghulam Narjis2 and Sabika Firasat1

...bodies level. Univariate analysis and odds ratios were calculated to determine the strength of association. The overall T. gondii infection was 60% with LAT, 52% IgG, and 56.26% with IgM ELISA. The seroprevalence was 7.2%-15% among the five study districts of KP province. The toxoplasmosis was found significantly (p<0.001) higher in 34.7% to 44.2% of pregnant women as compared to non-pregnant. The high prevalence of infection was recorded among women belong...

Moustafa M. Zeitoun 1,3; Mohamed M. Mansour 2,4* 

...m all camels for hormone analysis. Results; Blood testosterone level was lower (p < 0.05) in control “fertile” than that in infertile camels (3.05 ± 0.12 vs. 5.17 ± 0.24 ng/mL). Similarly, lower (p < 0.05) testosterone levels were found in the seminal plasma of control (1.29 ± 0.22 ng/mL) compared to infertile (2.78 ± 0.29 ng/mL) dromedaries. However, there were no differences (p > 0.05) in aldosterone levels be...

Rosidi Azis1, Gatot Ciptadi2, Sri Wahjuningsih2, Dwi Nur Happy Hariyono3, Yuli Arif Tribudi4, Veronica Margareta Ani Nurgiartiningsih2* 

... and multiple regression analysis were performed in a Matlab R2021a. The quality of fit of the models was evaluated using the coefficient of determination (R2) and root mean squared error (RMSE). The results showed that BW had a positively high significant correlation (P < 0.01) with HH (r = 0.756), BL (r = 0.754), and CG (r = 0.877). The stepwise regression results showed that the model using three predictors (BW = -159.57+0.44HH+0.69BL+1.38CG) was the bes...
Nabila Rasheed1, Shumaila Usman2*, Madeeha Sadiq3, Bushra Wasim3, Kanwal Haneef4, Hafsa Shafqat3, Tabinda Urooj3 and Saman Rashid4
 
...er characterization, the analysis of GDM effect on WJMSCS proliferation rate was analyzed by calculating the population doubling time of the cells at different passages (P0-P3). Moreover, the expression of the important cell cycle genes (CDCA2, CDCA8, CDC20, and CCNA2) was analyzed by quantitative PCR to determine the effect of GDM at gene level. The isolated cells expressed the stem cell specific marker CD90 which confirmed that the isolated cells are WJMSCs....
Hari Prashad Joshi, Kuaanan Techato, Khamphe Phoungthong* and Keshav Raj Panthee
...g (ARDL) Model. The data analysis from 1990 to 2019 showed that annual mean annual temperature and fertilizers have a long-term, favorable and significant impact on rice production. Whereas cultivation area has a short-term significant impact. Precipitation and cultivation area did not have a long-term effect on rice production. The study recommends prioritizing the expansion of rice cultivation area and area-specific practices for adapting to climate change f...

Imbang Dwi Rahayu1, Ali Mahmud1*, Wahyu Widodo1, Adi Sutanto1, Apriliana Devi Anggraini1, Devi Dwi Siskawardani2, Wisnu Nurcahyo3, Tri Untari3

...analyzed using a one-way analysis of variance. As a result, the treatments did not affect (p>0.05) the total plasma protein (TPP), globulin, cholesterol, high-density lipoprotein (HDL), aspartate aminotransferase (AST), and all parameters of the blood profile. However, the herbs’ addition significantly (p<0.05) affected albumin, triglycerides (TG), low-density lipoprotein (LDL), alanine aminotransferase (ALT), and malondialdehyde (MDA). Adding herb...

Mudawamah Mudawamah1*, Sumartono Sumartono1, G. Ciptadi2, E. Susanto3, Y. Hartoyo4, L. Affandhy5,6

...ntitative and laboratory analysis, with statistical analysis using ANOVA and LSD with SPSS Statistic 9 software. The research design used descriptive with total sample of 60 heads (morphometry and physiological status) and 24 heads (total protein).  There are three breeds of sheep: Sapudi, Dormas (Dormer x Sapudi), and Suffas (Suffolk x Sapudi). Sapudi, Dormas, and Suffas Ewes with only one child each lambing were abbre...

Peter Amuguni Mbato*, Wanzila Usyu Mutinda, Noah Kipkoech Maritim, Nicholas Edwin Odongo 

...nsitivity. Relative risk analysis (RR) was used to determine the association between confinement as a risk factor and bacterial infection. Birds in the FRPS had lower body weights that probably results less pressure on their skeleton. Treatment D birds had severe foot pad lesions (P < 0.05) compared to the other treatments at weeks 6, 7 and 8 and the severity decreased with increasing duration of FRPS. Complete confinement treatment was significantly associ...
Sri Rahayu*, Muhaimin Rifa’i and Widodo 
... a computational biology analysis to determine the role of GFD9 in mammalian ovarian function. We constructed a three-dimensional model of GDF9 using I-TASSER and human GDF9 protein sequences obtained from UniProt. We also analyzed GDF9 gene expression levels using GENEVESTIGATOR. Furthermore, we identified the proteins that interact with GDF9 using the Biological General Repository for Interaction Datasets and investigated their network formation using the ST...

A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2

...-red (FTIR) spectroscopy analysis and Transmission Electron Microscope (TEM). The rats (28 rats) used in this study randomly divided into 4 groups each of 7 animals; Control group, SeNPs-CP group (0.5 mg/kg B.Wt. / day /30 days orally), BPA group (50 mg/kg B.Wt. / day /30 days orally) and BPA- SeNPs-CP group. The results showed that BPA injection led to an obvious increase in the activities of liver enzymes, elevation of hepatic oxidative stress (a significant...

A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2

...-red (FTIR) spectroscopy analysis and Transmission Electron Microscope (TEM). The rats (28 rats) used in this study randomly divided into 4 groups each of 7 animals; Control group, SeNPs-CP group (0.5 mg/kg B.Wt. / day /30 days orally), BPA group (50 mg/kg B.Wt. / day /30 days orally) and BPA- SeNPs-CP group. The results showed that BPA injection led to an obvious increase in the activities of liver enzymes, elevation of hepatic oxidative stress (a significant...
Mohamed A. Abu El-Hamd1*, Abdelslam M. Metwally2, Zahya R.  Ghallab2, Wael A. El-Hamady and Mohamed A. El-karamany1
... low fertility. Economic analysis revealed that the PGF2-GnRH protocols were the least expensive (L.E. 65/animal), while the OvSynch treatment was the most expensive (L.E 95/animal).

...
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
...VR) of VP2 gene. Genetic analysis of the PCR amplicons has revealed that 3 samples belonged to very virulent IBDV (vvIBDV). The genotyping of Egyptian vvIBDv indicate progressive evolution compared with previously isolated strains which indicates persistence of vvIBDv in Egyptian poultry environment. In conclusion, theses data reveal the success and nonstop evolution of the vvIBDv in the Egyptian environment. This is why we must do further

Kandiah Pakeerathan*, Konesalingam Jeyavithuyan, Aruchchunan Nirosha and Gunasingham Mikunthan

...t and Person-correlation analysis were performed to identify the best treatment combination at P < 0.05. The results were significant among them at P < 0.05. Bio-fortified vermicompost’s pH range was 7.28-8.11, C/N ratio was 25.01-9.96, and OMC was 22.66-51.53. Nitrogen, P and K ranges were 1.06% to 2.1%, 0.73%-1.87%, 0.654% to 1.38%. Trichoderma viride + P. fluorescens enriched showed lower DSI. The highest growth, yield parameters, and lower Fusa...

Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1 and Iqra Nazir3

... was confirmed using PCR analysis. Afterward, the growth kinetics of the mutant was compared with parent RB51. The mutant bacterial growth significantly reduces (P< 0.05) as compared to the parent non-mutant confirming its auxotroph inability towards purine pathway biosynthesis which serves as a crucial factor for disease induction and survival inside the host system revealing enhanced attenuation in the deleted mutant. However, after supplementation of pur...

Jemi Diporianto1,4, Denny Widaya Lukman2*, Yusuf Ridwan3 

...lth Code (TAHC) for risk analysis. The entry assessment was based on the Biosecurity Import Risk Analysis Guidelines 2016 Australia, while the uncertainty level was determined based on the guidelines provided by The European Food Safety Authority (EFSA). The final assessment of the risk of Trichinella spp. introduction through the transportation of wild boar meat via Bakauheni Port indicated a moderate likelihood with low un...

Shaista Ilyas1, Safdar Ali1, Amer Habib1, Misbah Ali1,2, Muhammad Ahmad Zeshan3*, Yasir Iftikhar3, Muhammad Usman Ghani4,5 and Muhammad Umair1

...rrelation and regression analysis were performed to find the relation of environmental factors and disease development. There was a positive relationship between disease severity (Cercospora leaf spot disease) and relative humidity, wind speed and rainfall. The results revealed that there was negative correlation between maximum temperature and disease development. It could be concluded that the application of nutrients alone and in combination caused signific...

Arnold Christian Tabun*, Ferdinan Suharjono Suek, Cardial L Leo Penu, Johanis A. Jermias, Thomas Lapenangga  

...ng district based on DNA analysis using the cytochrome b (CYT B) gene. The methods used CYT B gene base methods of DNA analysis such as DNA extraction, Polymerase Chain Restriction, DNA sequencing, and identification of DNA sequences nucleotide. Bali cows with sorrel, black and white coat colors are used with an age range of 3-5 years. DNA sequencing conducted multiple alignments, the identification of the genetic distance, ...

Kyu-Jung Kim and Shin-Jae Rhim*

...ltiple linear regression analysis revealed close relationships between stream structure variables and major waterbirds. Waterbird community conservation requires wetland-based management programs that are applied to habitat complexes at the landscape scale to support the needs of waterbirds. The results of this study can help with the conservation of habitats and landscapes in urban streams. Further research should be conducted on the conservation of waterbird...
Burarah Arooj1, Zeeshan Mutahir1, Moazzam Ali1, Mohsina Akhter2, Malik Siddique Mahmood1, Arslan Hamid3 and Mahjabeen Saleem1*
...e protein-ligand docking analysis and molecular dynamic simulation indicated that the interactions of amino acids Asn 204, Glu 277, Leu 278, Asp 312, Glu 314, Gln 372, Tyr 374, Trp 463, Trp 467 from GH18 catalytic domain with (NAG)2 molecule are involved in the enzyme catalytic mechanism. This is the first time a thermostable chitinase from Paenibacillus sp. Y412MC10 has been cloned, expressed heterologously, and purified.

...

Nida Sadaqat1, Sheheyar Ahmed Khan1, Amna Bibi1, Sadaf Zahra1, Muhammad faisal Salamt1, Noreen Latief2 and Fatima Ali1*

...°C for PCR and ELISA analysis. The therapeutic effects of a NAC supplementation for a short period of time on rat skin wound healing were investigated. The NAC treatment promoted an improvement of wound healing by reducing the oxidative stress, improving wound closure, reducing tissue inflammation with no significant change in blood parameters. There were no significant differences in weekly and final body weights and no significant changes observed in hem...

Nadeem Akmal1, Abid Hussain1*, Muhammad Yousaf2, Waqar Akhtar1 and Hassnain Shah1

...ade by applying economic analysis technique. As, Basmati-Super and Basmati- 386 are main rice varieties grown in the country. Thus, this study is based on data of 46 purposively selected farmers for the crop season 2021, including 30 and 16 farmers of Basmati-386 and Basmati-Super, respectively. Cost comparison of the crop produced through mechanical and conventional manual transplanting techniques revealed that costs of raising the nursery and its transplanti...

Omar Berkani1*, Souheila Slimani2, Nora Sakhraoui3 and Cherif Abdennour1

...ion, vas different semen analysis and plasma FT4 level were measured at week 10. Results showed that under long photoperiod, the sexual activity of the control group lasted for 06 weeks, characterized by a significant growth in the testicular volume, followed by spontaneous gonadal regression up to week 10. Azoospermia in control birds was noticed, with a significant increase in FT4 level and a fall of flying feathers in the 8th and 10th weeks, which assert th...

Saad Tahir, Nadeem Ahmed*, Mohsin Ahmad Khan, Muhammad Akram, Rabia Abbas and Kausar Malik

...estern blot, and RP-HPLC analysis were also conducted, which confirmed the 98% purity of the product with 40% yeild having a molecular weight of almost 111kDa. The purified fusion protein (HSA-SK) was also verified via western blotting by using anti-streptokinase antibodies. Clot lysis assay was used to estimate the biological activity of HSA-SK. For half-life estimation, proteolytic and thermal stability of HSA-SK was also checked.

...

Saime Betül Baygeldi1, Barıs Can Güzel1*, Ramazan Ilgün2 and Zait Ender Özkan1

...lectron microscopy (SEM) analysis. In addition, by applying the silicone plastination technique to the tongue, it is aimed to compare the SEM images before and after plastination. German mast geese used as study material were obtained from goose breeders. After the micro-anatomical features of the German goose tongue were determined, SEM (FEI-Quanta; FEG 250, USA) analyzes were made and their general structures were photographed. Then, silicone plastination pr...

Dhurgham K. Seger1, Rahman H. H. Al Qasimi2, Azhar A. Jaffar3, Salah H. Faraj4, Ahmed I. Ateya5* 

...f the multiple alignment analysis of the sequence of SSTR1 gene indicated the presence of one mutation 264 A>G. The obtained mutation produced three genotypes, namely AA, AG, and GG. The frequency of the genotypes AA, AG and GG were 0.50, 0.17 and 0.33, respectively. While the frequency of the A and G alleles were 0.58 and 0.42, respectively. There were significant impacts of SSTR1 gene polymorphisms and the weights of carcass characteristics including hear...

Nazia Haider, Asia Bibi* and Nahid Kausar

... Anti-B and Anti-D). The analysis of haematological indices was done by using automated haematological analyzer (Sysmex Co. Japan). The mean maternal age in this study was 30.35±7.94 years for cases and 28.53±6.59 years for controls. Highest frequency of miscarriages 90 (30%) was observed in cases of age group 35-39 while lowest frequency of miscarriages 30 (10%) was observed in age group 20-24. Whereas, highest frequency 94 (47%) and lowest 2(1%...

Di Zhou1, Qingmeng Long1*, Rong Yang1, Xiaoshan Tan1, Jun Li1, Mingyan Tang1, Zhonghai Zhao3, Ye Ao2, Zhinan Zhou1 and Changxue Chen1

...justify;">We used RNAseq analysis to identify key genes affecting reproductive traits for the Guizhou black goat. We utilized mRNA collected from uterine, ovarian and fallopian tube tissues of goats with the same pedigree and analyzed the results using bioinformatics and qRT-PCR. We found 8120 differentially expressed genes (DEGs) in ovarian tissues (3205 up- and 4915 down-regulated), 5255 DEGs in the fallopian tubes (1317 up- and 3888 down-regulated) and 5180...

Wei Fang1,2,3, Jiawei Hong1,2,3,4, Zhengyi Fu1,2,3, Jing Hu1,2,3, Gang Yu1,2,3, Zhenhua Ma1,2,3* and Humin Zong5*

...pathways and SSR feature analysis. The results showed that a total of 42.40 Gb data were obtained from transcriptome sequencing, and 38,817 Unigenes were obtained after assembly and de-redundancy; Using BLAST to annotate the assembled unigenes with seven functional databases (NR, NT, GO, COG, KEGG, Swissprot and Interpro), a total of 23,417 unigenes were annotated (accounting for 61.14%); A total of 253 differentially expressed genes (DEGs) were screened, with...

Khadija Begum, Azizunnessa and Md Ahaduzzaman*

...multivariate statistical analysis was done to study the prevalence and associated risks of different reproductive disorders. Results show that 12.41% (382/3079) reproductive disorders in does were recorded during the study period. The major reproductive disorders were anestrous (72.25%, 276/382), followed by retained placenta (6.8%, 26/382), and repeat breeding syndrome (4.19%, 16/382). Significant variation in prevalence estimates was observed considering the...
Muhammad Abdul Mannan1,2, Sharmin Chowdhury2, Md Abul Hashem3,4 and Md. Hazzaz Bin Kabir1,5*
.... Through the sequencing analysis, 45 nad4 genotypes and 10 ITS-2 genotypes were discovered. The prevalence of Haemonchus contortus isolated was not influenced by demographic factors such as age, gender, breed, and seasons in the study. Our results highlight the state of our knowledge on the spread of H. contortus parasite among small ruminants in Chattogram slaughterhouses.

...

Nabi Ullah, Abdul Samad Mumtaz*, Lubna Anjum Minhas, Muhammad Kaleem, Rooma Waqar, Amber Jabeen and Ayesha Hanif

...d water physico-chemical analysis revealed that phytoplankton diversity greatly affects by various factors like Total soluble salts, Total Alkalinity, pH, TDS, EC, Temperature, Carbonate CO3-2, Bicarbonate HCO3-1, Chloride Cl-1, Sulphate SO4-2, NO3-1, Ca+2, Mg+2, Na+1, K+1, BOD and COD, Turbidity. The supreme supportive water physico-chemical parameters for the algal development was recorded are the Temperature, pH, and the presence of nutrients like NO3-1, Na...

Komal Mukhtar*, Saba Yasin, Husna Ahmad, Muhammad Amir Iqbal and Nabila Roohi

...was used for statistical analysis. There was a significant (P < 0.05) decrease in RBC in the painters as compared to the control group. Red cell indices such as HGB, HCT, MCH and MCHC were significantly (P < 0.05) reduced in painters. On the contrary, PLT, MPV, WBC, LYM and Gran count were substantially (P < 0.05) elevated in the painters. In conclusion, chronic paint exposure predicts future illnesses such as anemia and cancer. 

...

Aryaf Mahmood Sabea1*, Majida A. Al-Qaiym2 

...ks. Immunohistochemistry analysis showed that expression of CD4, CD8, and CD19 on Tand B lymphocytes and CD68 on macrophages were affected inversely by hypothyroidism. Selenium supplementation synergies levothyroxine in reestablishment the activity of lymphocytes and macrophages. In conclusion, the present study demonstrated that thyroid hormones had regulatory role for innate and adaptive, and in order to reach the best result of treating hypothyroidism an...

Mustafa M. Khalaf*, Rana A. Salih 

...ollected identically for analysis of the liver enzymes and hepatic oxidative stress indicators. Cyclophosphamide significantly increased malondialdehyde (MDA), liver biomarkers (ALT, AST, ALP, and TP), and prothrombin time. Otherwise, glutathione (GSH), and total protein (TP) level was substantially reduced in the control group. Studies have demonstrated quercetin’s capacity to decrease cyclophosphamide’s effects on (MDA, ALT, ALP, TP, and AST...
Qiangsheng Wu1, Yumei Yin2, Hui Gao1, Baoding Chen3, Jianjun Yu2, Jian Zhang4, Donglai Zhu5, Fang Liu6, Guohong Ge7 and Ya-Mei Dang8*
...d through bioinformatics analysis. The results showed that the functions of these 1495 differently expressed genes enriched in cell cycle, oxidation-reduction and drug metabolic process. Additionally, the top 10 core genes of these differently expressed genes were mainly participated in cell cycle and strongly correlated with worse prognosis of survival, which may be potential diagnostic and therapeutic targets of HCC.

...
KHALID JAVED IQBAL1, ARSHAD JAVID2, NOOR KHAN3, IRFAN BABOO4, AHMED ALI5, KASHAF
MOHSIN6, AZRA ANWER7, USMAN ATIQUE8, MUHAMMAD ALTAF9, DILAWAR HUSSAIN10 & ASMA
CHAUDHRY11
.../div>
survey data, analysis techniques were carried out to achieve attitude
measurement for a set of scales. Significant percentage effects were
found with age, profession and education of fish consumers. Percentage
of those fish consumers found to be higher which were with age of <25
(43%), master passed (40%), students (49%). Quality, quantity and
effectiveness of fish consumption were also...
MISBAH RAZZAQ, MUHAMMAD RAIS*, MUHAMMAD ARSLAN ASADI, SALEHA ABBASI &
ABDULLAH IBRAHIMv
...ntent flushing and fecal analysis. Of the recorded 17
dietary items, ten (59%) were of plant origin and seven (41%) were of
animal origin. Pyrus pashia (80%) and Isodon rugosus (0.37 ml) were
recorded as the most frequent plant species in fecal samples and
stomach contents, respectively. The majority of insects consumed by the
lizard species were Coleopterans (0.22 ml) followed by Hymenopterans
(0.18 ml) and Hemipteran...

RUBAB ZAFAR KAHLON*& IBTISAM BUTT

...
suggests a detailed analysis of the possible environmental and
anthropogenic factors which played a role in the decline of bird richness
in the study area.
...

SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN

...ents
...

MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR

... be used for the routine analysis of
levofloxacin in injection dosage form. The proposed method is free
from expensive solvents, chemicals and time consuming steps and
can be used in quality assurance laboratories of pharmaceutical
industries.
...

Salam N. Aritonang*, Elly Roza, Rizqan, Winda Habsani 

...nd shoulder height. Data analysis was carried out descriptively based on arithmetic mean, standard deviation, and coefficient of variance. The result showed that the body weights of male and female buffalo crosses at <6, 7−12, 13−18, and 19−24 months were 210.26 and 196.48, 304.14 and 288.42, 399.46 and 394.12, and 430.85 and 423.16 kg, respectively. The chest circumference of male and female buffalo crosses at ages ≤ 6, 7−12, 13&...

Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1 

...ntration. Phytochemicals analysis indicated the presence of alkaloids, phenol, saponins, flavonoids, tannins, and glycosides. The test organism was more sensitive to C. limonum compared to C. aurantium. The MICs ranged from 1.56 to 12.5mg/ml; MBCs, (3.125 - 25mg/ml) and MBC/MIC ratios (2.0-4.0). Furthermore, a time killing curve of each 4x MICs and 8x MICs concentrations of juice extract showed a distinguished bactericidal effect at 6th hr. Therefore, the use ...

Abdul Latif Rind1*, Shahid Hussain Abro1, Rameez Raja Kaleri2,3, Ghulam Mustafa Solangi4, Raza Ali Mangi5, Muhammad Anees Memon6, Depeesh Kumar Bhuptani7, Sana Noor8, Zainab Lanjar9, Zahid Ali Mangrio10 and Abdul Wahid Solangi

...entify PPR antigens. The analysis of PPR prevalence was carried out according to breed, sex, age, and Taluka. The overall PPR prevalence was found to be 20%, with the highest prevalence rate (50%) observed in Jam Nawaz Ali Taluka and the lowest (7.5%) in Shahdadpur Taluka. The prevalence was higher in goats (30%) than in sheep (10%). Female animals had a higher prevalence than males, with a prevalence of 25% in female goats and 7% in female sheep. The prevalen...

Hazrat Usman1, Shakeel Ahmad2*, Salah Uddin3, Humaira Wasila3 and Yasser Durrani4

...ach Tehsil. The research analysis focused on various parameters, including pH, turbidity, electric conductivity, total hardness, sodium, potassium, calcium, magnesium, carbonates, bicarbonates, chlorides, and fluorides. The recorded average value ranges for each parameter provided insights into the characteristics of the well water samples. For example, pH values ranged from 6.87 to 7.53, indicating acidity or alkalinity levels. Turbidity, a measure of water c...

Qasim Ali1, Mudssar Ali1*, Muhammad Awais Ahmad1, Asif Sajjad2 and Shafqat Saeed

...scutellaris. Comparative analysis revealed that fruit dimensions, weight, pulp content, and seed count per fruit were notably improved in instances where insect pollination was facilitated, in contrast to cases where no insect visits were allowed. Furthermore, chemical properties were also found to be superior in scenarios involving free insect visits. The study underscored the efficacy of A. dorsata and syrphid flies (E. balteatus and I. scutellaris) in terms...

Abdiaziz Idiris Mohamud1,2*, Yonis Abukar Mohamed1,3 and Shafii Abdullahi Mohamed1,4

...ntextual considerations, analysis of different production systems, and exploration of the intersection of animal welfare and zoonotic disease prevention, is necessary for evidence-based decision-making. By leveraging research, promoting evidence-based interventions, and strengthening policy and governance frameworks, Somalia can enhance animal welfare, support sustainable livelihoods, and contribute to overall sustainable development in the country. The integr...

Ali Mosa Rashid Al-Yasari1, Zahid I. Mohammed2, Haider S. Almnehlawi3,4, Ali F Bargooth5, Nawar Jasim Alsalih1, Huda F. Hasan6*, Mohenned A Alsaadawi1 

...ups, immunohistochemical analysis revealed a significant reduction in insulin secretion cells. In contrast, considerably more glucagon-producing cells were seen in the STZ group. Data from the camel milk group demonstrated its capacity to lessen the effects of STZ, but it did not completely eliminate the consequences of diabetes as there were still some disparities between the camel milk group and the control group. In conclusion, although camel milk didn&rsqu...

Hui Fang*, Kai Xue and Teng Yang

...twork. In this study, an analysis of miRNA-mRNA networks was conducted to identify miRNAs linked to autosomal dominant polycystic kidney disease (ADPKD). Fifty RNA samples from Gene Expression Omnibus (GEO) database were obtained to identify differentially expressed miRNAs (DEMs) and genes (DEGs) in the progression of ADPKD. Functional annotation and enrichment analysis of DEG were performed. iRegulon was used to construct t...

Jyotsana Shakkarpude1*, Aditya Mishra2, Deepika D. Caesar2, R.K. Sharma3, Anand Kumar Jain2, Sanju Mandal2, Rajesh Kumar Vandre4, Danveer S. Yadav5, Bhavna Ahirwar6 and C.P. Solanki6

...ollected from animal for analysis of superoxide dismutase, total antioxidant capacity, HSP70, albumin/globulin ratio and immunoglobin G. The overall mean values of plasma superoxide dismutase, total antioxidant capacity and immunoglobin G of high betaine group was numerically higher than other groups which differed (p<0.05) significantly between groups. The overall mean value of plasma HSP70 of high betaine group was minimum followed by low betaine group an...

Shafi Muhammad1, Bibi Nazia Murtaza2, Aftab Ahmad1, Muhammad Shafiq3, Nurul Kabir4 and Hamid Ali1*

...pecially chemokines. IHC analysis revealed significant changes in the architecture of liver especially an abnormal expression of alpha (α)-SMA and the activation and transmigration of Kupffer cells towards the septa.

...

Sidra Mumtaz1, Sidra Arshad1, Rizwan Ullah Khan1, Muhammad Usman Rashid2 and Naila Malkani1*

...clear. Therefore, a meta-analysis was performed to analyze their aberrant expression in breast tumorigenesis. A thorough literature search was performed and 73 relevant studies were identified which were further scrutinized and finally 26 studies covering 4553 subjects were included for analysis. A random effect model was applied and correlation was calculated using odds ratios (OR) at 95% confidence intervals (CI). Combined...

Lu Liu1, Xiang Zhao2, Yue Qin2, Tianxiang Gao3 and Tianyan Yang3*

...tification. Phylogenetic analysis based on PCGs suggested that genus Lophius was more closely related to Lophiodes than Antennatus.

...

Wafa Akram1, Shahan Azeem2, Shafqat Shabir1, Haroon Akbar1*, Warda Gill3 and Muhammad Imran Rashid1*

...ed by PCR and sequencing analysis. DNA sequencing of the amplicon revealed close homology with Leishmania sequences available in GenBank. Alignments and phylogenetic analyses of the Leishmania infantum from a tiger in Pakistan indicated 94-100 % identity with Leishmania from animals and 98.8-100% with Leishmania from humans origins suggesting the need for screening of animals before transporting, and of humans before taking care of captive animals, in order to...

Jaweria Iqbal1, Muhammad Tanveer Altaf2, Muhammad Faheem Jan3, Waqas Raza4*, Waqas Liaqat5, Ikram ul Haq6, Amna Jamil7, Sameer Ahmed1, Amjad Ali2 and Arif Mehmood8

...for SSR primers. Cluster analysis revealed a high level of genetic similarity for ISSR (average 0.92) and EST-SSR (average 0.85) among cotton genotypes. The mean polymorphic information content (PIC) value was 0.25 for ISSR, whereas it was recorded 0.48 for EST-SSRs. Confusion probability (Cj) exhibited a negative association with discriminating power (Dj), while Dj displayed a positive association with PIC. Marker discriminating statistics showed that EST-SSR...

Nur-E-Farjana Ilah1, Md. Joynal Abedin2, Esmout Jahan Alice1, Taiba Akter Laboni1, Mst. Shahinur Khatun1, Most. Shakila Sarmin1, Md. Ashekur Rahman1, Obaidur Rahman1, Md. Akhtarul Islam1, Muhammad Ali Fardoush Siddquy3 and Md. Yeamin Hossain1*

...h (b~3). The statistical analysis conducted in this study revealed a highly significant LLR (p<0.001) with an r2 value exceeding 0.944. The form factor (a3.0) was found as 0.0155, while the size at first sexual maturity (Lm) was determined as 12.36 cm TL. The natural mortality (Mw) was calculated as 1.57 y-1. A major connection between KR and BW for this species, indicating that KR was an exceptionally significant in revealing the health of A. atropos. Ther...

Slamet Widodo1*, Mohammad Ikhsan Shiddieqy1, Teguh Wahyono2, Yeni Widiawati1, Zultinur Muttaqin1

Mervat M. Fath- Allah, Amal A. Ahmed, Hala A. Amin

... /> nucleotide sequence analysis of the polyprotein gene of PVY-ME2 (the present Egyptian isolate)
showed 98% homology with the PVY-isolate strain N of Poland. In turn the PVY-ME2 showed low
homology with the other characterized Potyvirus members. Furthermore the PVY-ME2 had much
more distantly related (91%) to the previously published Egyptian and France isolates. The WMVME2
isolate (Egyptian isolate) has a high similarity (98%) w...

Lawrence Olusola Oparinde1*, Adewale Isaac Olutumise2,3 and Ademola Adegoroye

...del and Gini coefficient analysis. Findings from this study revealed that the adoption of agroforestry technology increased income inequality among male and female crop farmers. Also, the difference in the income inequality level between adopters and non-adopters of agroforestry technology among female farmers was more than what was obtained among male farmers. Income inequality among female crop farmers was more than that of male crop farmers, while more male...

Shafqat Ullah1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Raheela Taj3, Fatima Syed3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Muneeb Islam7 and Sumaira8

...ca Inc). The statistical analysis was through IBM SPSS (Statistics.V22.X86). The present study concluded that selenium improves the intestinal morphology i.e. villus height, villus width, villus surface area showed better growth(P≤0.05) in the selenium supplemented groups. The FCR initially did not differ (P≥0.05) among the groups but toward the end it was comparatively better (P≤0.05) in selenium supplemented groups. The relative body weight of diffe...

Siddique Ahmad1*, Basit Ullah1, Sajid Ali2, Ali Zaid1, Zeeshan Ahmad3, Muhammad Usaid1, Muhammad Zeeshan1 and Saif Ullah3

... irrigation. Statistical analysis was done combined over bio-aab. The principle beneficial microorganisms (BM) in bio-aab contain photosynthetic bacteria, lactic acid bacteria and yeast. These BM produced useful substances such as amino acids, nucleic acids, lactic acid, bioactive substances, hormones, enzymes and sugars. All these substances promote active cell and root division, plant growth, productivity, suppress harmful microorganisms, and enhance the dec...

Salma Sharif1, Rana Arsalan Javaid2*, Abid Majeed2, Muhammad Shahzad Ahmed2, Qurat ul Ain Sani2, Faiza Siddique2, Muhammad Arshad3 and Niaz Ali

...hed those of the cluster analysis quite closely. Breeders may now use these findings to create high-yielding rice varieties as well as novel breeding techniques for rice development.

...

Shiferaw Demissie Tola1, Diriba Muleta2, Fassil Assefa2 and Beira Hailu Meressa1*

...ge; 8 J2 (g soil)-1. The analysis of Seinhorst’s yield loss model indicated the highest tolerance limit (T=0. 64 egg + J2 (g soil)-1) recorded for leaf number, while the relative minimum yield (m) of 0.91 and 0. 86 were the highest m values for root length and shoot height, respectively. Furthermore, the maximum multiplication rate (a) and population densities (M) were estimated as 8813.2 and 3420.1 (eggs and J2 (g soil)–1), respectively. Therefore...

Michelle Anne Diano1,2, Loel Dalan1,2, Neil Pep Dave Sumaya3 and Nanette Hope Sumaya1,2*

...cal characterization and analysis of the D2-D3 expansion segments of the 28S rDNA sequence. The D2-D3 rDNA sequence alignment of the four isolates, B2R1 (MT459236), B2R2 (MT459237), B2R3 (MT459238), and B3R1 (MT459239), do not exhibit any nucleotide differences with each other or with previously described populations of C. briggsae from China (MN519141), Germany (EF417140), and the USA (AY604481, JN636061); therefore, they are conspecific. This is the first re...

Moazzam Baig*, Sohaib Ahmed and Arz Muhammad Umrani

... and through descriptive analysis while collecting data from core area. Despite the fact that many individuals go to urban places to work, such as Dubai and the Arab Emirate. Further, statistics and reports show that the government has made a little advancement in the area’s growth in a few successive years. They don’t have any clear guidelines for protecting the forest in the Sherani district. The people are using the forest resource to meet their...

Aamir Shakeel1*, Anwar Ali2, Tahir Iqbal1 and Ziad Raza1

...e changing landuse. This analysis examines the dynamics of Land Use Land Cover (LULC) changes in Kaghan Valley between 2004 and 2012 with range of observation of 4 years i.e., 2004, 2008 and 2012. The study reveals distinct shifts in various LULC categories, encompassing cropland, forest land, grassland, other land, settlements, and wetlands. Notable trends include a decrease in cropland area by 13.72%, possibly due to urbanization and shifts in agricultural p...

Mahfouz M.M. Abd-Elgawad 

...r parametric statistical analysis. Basically, they can help in unraveling the complexities of ecosystem dynamics into quantifiable metrics. Yet, their merits and demerits, discussed herein, should be considered by researchers and stakeholders. As they should be tailored to fit the intended goal of application, related modifications to derive more accurate and appropriate conclusions for nematode management are reviewed in this study. For instance, iteration im...

I Nyoman Sumerta Miwada1*, I Nyoman Sutarpa Sutama1, Agus Susilo2

...arch method used one-way analysis of diversity (at p<0.05) and continued with the Duncan test using the SPSS version 25 system. The results showed that increasing the level of Moringa leaf extract had no effect on pH, water content, and fat but significantly affected protein and ash content (p<0.05). In the color aspect, the L* and a* values ​​significantly decreased (p<0.05), but the b* values ​​significantly increased. Texture profile

Roberto Gallegos-Acero1, Ali William Canaza-Cayo2,4*, Francisco Halley Rodríguez-Huanca1, Rubén Herberht Mamani-Cato3, Abigail de la Cruz1, Júlio Sílvio de Sousa Bueno Filho4 

...e method of genealogical analysis method. ENDOG program v.4.8 was used for the calculation of the following parameters of the genetic structure such as: average inbreeding coefficient (F), average relatedness coefficient (AR), the effective numbers of founders (), effective number of ancestors (), generation interval (GI) and the pedigree completeness, the ENDOG program v.4.8 was used. The F and AR were 0.06% and 0.40%, respectively, The number of ancestors th...

Nurul Isnaini*, Dedes Amertaningtyas, Hanief Eko Sulistyo, Aulia Puspita Anugra Yekti, Faizal Andri

...(starter). A statistical analysis was conducted using analysis of variance using Proc Mixed with general linear model (GLM) using SAS studio for academics Online Edition (https://odamid-apse1.oda.sas.com/SASStudio/). Comparing two results, it can be seen that the different enriched media gave gradually significant differences (p < 0.05) in the egg weight, number of eggs of BSF success to hatch, hatchability, length, width...

Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2

...the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4th day of observation showed that the addition of ascorbic acid as NEZ at a dose of 20 mg/100 ml into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentag...

Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2

... were analyzed using the analysis of variance (ANOVA) followed by Duncan’s multiple range test to determine the difference among the treatments. The current study showed that adding 15% sugarcane (T2) improved the semen parameters compared to the other treatments with 54% progressive motility, 74.8% viability, and 8.8% total abnormalities at 5°C for 4 days of storage. 15% sugarcane juice in egg yolk tris-buffer diluent preserves Kacang goat diluted s...

Irfan Safdar Durrani*, Noreen Asim and Ammar Sohail

... and sequenced. Sequence analysis using Plant Care online database revealed 32 different regulatory elements in Nipponbare while 31 different regulatory elements were found in Dilrosh-97. The cis-acting elements were found to be involved in plant hormonal inductions, developments and biotic and abiotic stress response. Notably, the OsGLP12-3 gene promoter from Dilrosh-97 was found to contain higher number of TATA-box and CAAT-box as compared to Nipponbare, ref...

Qi Jiayi1, Huang XuMiao2, Lin Wei3, Ao YaHui3, Zhao Wenting2* and Cao Chunhao3*

... KEGG pathway enrichment analysis genes were given functional identification. In total, there were 102 differentially expressed immune-related lncRNAs which were identified, and there were four genes (USP30-AS1, U62317.1, ZEB1-AS1 and MIR205HR) which were screened from the gene signature to form a prognostic signature model. Based on the survival analysis results, patients with high-risk scores had poor survival outcomes. To...

Abir Ishtiaq1*, Abdul Ghaffar2, Maria Younas1, Tasveer Ishtiaq1, Zara Naeem3 and Muhammad Naeem4*

...a private fish farm, for analysis of body composition parameters’ variables in relation to body size and condition factor. Mean body weight and total length was measured and condition factor of farmed collected fish was calculated as 556.45±28.24 g, 34.78±0.60 cm and 1.30±0.02, respectively. On wet weight basis, mean percentage for water, protein, fat and ash was found 74.70±0.76%, 16.92±0.66%, 4.60±0.16% and 3.7...

Tanvir Hussain1,2*, Kafeel Ahmad2, Muhammad Bilal Zia1, Hammad Uddin1, Khalid Hussain1, Muhammad Inamulla Khan3 and Said Akhtar Khan1

...opulations while cluster analysis among the populations clearly showed two groups i.e., BOR with NAR and BAR with MLJ. The population of ANP was grouped separately with the cluster of BAR and MLJ. The AMOVA analysis revealed high genetic variation (90%) within and low (10%) among populations. Further, Mantel’s test explored that geographic distribution was not correlated to genetic distances among the populations. The ...

Afshan1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Tahira Tayyab1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Syed Weqas Ali7 and Assad Ullah8 

... centrifuged for further analysis. The redox status and thyroid hormones were measured through commercially available kits. The current study resulted that selenium supplementation improved(P≤0.05) the catalase level in the liver and muscles which fight against the free radical and reduce oxidative stress. In the blood serum the catalase and Malondialdehyde (MDA) both shows significant (P≤0.05). In conclusion I assume that 0.3mg/kg of selenium supplement...

Imtiaz Khan1*, Abdullah1, Muhammad Ibrahim1, Muhammad Ishfaq Khan1, Saima Hashim1, Shomayela Afzal2 and Khalid Nawab3 

...nefit ratio (%).The data analysis revealed that the herbicide Pendimethalin showed the lowest A. tenuifolius Cav. density (6.10 m-2), fresh weight (1.30 kgm-2) and dry weight (0.43 kgm-2) , while the control plots had the highest density, fresh weight and dry weight of A. tenuifolius Cav. Data regarding chickpea parameters, Pendimethalin showed the maximum plant height (46.66 cm), number of nodes (32.33 plant-1), number of branches (10.33 plant-1), leaf area i...

Waqas Ahmad*, Muhammad Naeem

...hondrial COI gene. BLAST analysis confirmed 100% identity of Notopterus notopterus with reference sequences belonging to NCBI GenBank database. Genetic diversity was analysed by using PCR amplification of 75 specimens of N. notopterus with five RAPD markers. RAPD markers data indicated high genetic diversity 0.5832 in Satluj while lowest genetic diversity 0.2173 in Chenab. High genetic polymorphism 60.52% observed in Satluj while lowest genetic polymorphism 23...
Waheed Ahmad1, Muhammad Tayyab1*, Bushra Muneer2*, Abu Saeed Hashmi1, Mansurud Din Ahmad3, Shagufta Saeed1, Muhammad Nauman Aftab2, Sehrish Firyal1, Muhammad Wasim1, Muhammad Azam4, Muhammad Talha5 and Ali Raza Awan1

Kristina Morkūnienė*, Rūta Insodaitė, Laimutis Kučinskas, Renata Bižienė 

...tion method. Statistical analysis was performed with SPSS Statistics 20 and PLINK software. SNP at position 7454459 (Asn → Thr) of Mblk-1 gene mutant allele A was more common in untreated domestic and wild honey bees (18.90% and 8.14% respectively; p=0.001) compared to treated domestic bees which persistently infected with the disease. Regression analysis showed that recessive AA genotype of this polymorphism significan...

Mukkram Ali Tahir1, Noor-us-Sabah1*, Muhammad Waryam Warraich1, Ghulam Sarwar1, Muhammad Aftab2, Fakhar Mujeeb3, Muhammad Zeeshan Maznoor1, Aneela Riaz4 and Sarfraz Hussain2

... carried out followed by analysis in the laboratory. All collected data was statistically analyzed for interpretations. It was depicted from results that use of carbon sequestering fertilizer (press mud @ 1 %) along with commercial K fertilizer (SOP) performed superior by resulting in maximum K content in shoot (2.13 %), K content in root (1.12 %) and K content in soil (330 ppm). Similarly, highest fresh weight (42.43 g) and dry biomass (14.37 g) of wheat were...

Muhammad Nauman Arif1, Muhammad Mansha1* and Tanveer Hussain2

...riation and phylogenetic analysis using ATPase 6/8 genes of mitochondrial DNA. The blood samples from hog deer were collected and DNA was extracted by using in-house organic methods. PCR was used to amplify a total of 880 bp of overlapping genes, which were then sequenced. Bioedit software was used to align, and edit the sequences, and single nucleotide polymorphisms (SNPs) were evaluated. The MEGA X-based maximum likelihood method was employed to study the ge...

Faiza Naeem1, Muhammad Farooq Sabar1, Muhammad Usman Ghani2,*, Qurat Ul Ain1 and Qurat-ul-Ain Zafar1

...gh complimentary network analysis. Protein-protein interaction studies were conducted using String (https://string-db.org/) and genes were ranked on a centrality score basis using Cytoscape (https://cytoscape.org/index.html). IL4, IL13, IL10, IL27, ADAM33, TBXA2R, FCER1β, ORMDL3, IKZF3, LRRC3C, GSDMA, GSTP1, GC, STAT6, CD14 and ACE are asthma-associated genes, significantly reported in the Pakistani population. Pathway-connector provided a network of fort...

Hassan Anwar1, Talha Anwar2, Khurram Muaz­3, Muhammad Riaz4* and Rabia Anwar5

.... We applied statistical analysis and image analysis. Statistical significance results are achieved using MANOVA. The image analysis for texture revealed similar results as those observed for chapatis made from similar flour. Our image analysis also proved the gluten’s sticky binding property, i.e., filling the porous structure of chapatis.

...

Aniqa Nasir1, Rashid Masih1, Shazma Massey1*, Laiba Arshad2, Sabi Ur Rehman2, Irva Waqar1 and Anwar Khalid3

...is determined by thermal analysis by using advanced isoconversional method. Isoconversional method is used to determine kinetic triplet including activation energy, pre-exponential factor and mechanism of thermal degradation. The prepared dressings were subjected to thermal analysis. Thermogravimetric analysis showed that the degradation of wound dressing occurs at 225˚C - 400˚C. Thus, t...

Khanitta Pengmeesri1,2* Thassawan Somchan1 , Doungnapa Promket1,3 

...uality records underwent analysis with four treatments: 10 weeks age of CBN (10CBN), 12 weeks age of CBN (12CBN), 10 weeks age of YWN (10YWT), and 12 weeks age of YWN (12YWT). Findings highlight distinctive shear force in breast meat across breeds, with YWN displaying higher levels. Younger chickens show more cooking loss in the breast. While thigh breed effects are absent, shear force fluctuates with age, higher in older birds. Meat quality between purebred a...

Sara H. Zughayyar*, Amer H. Gyad 

...Subsequent phytochemical analysis revealed the presence of tannins, carbohydrates, phenols, resins, flavonoids, saponins, alkaloids, coumarins, terpenes, and glycosides, while steroids and proteins were absent. The extract displayed antidiarrheal activity comparable to loperamide, showing a significant reduction in intestinal content volume and weight (P≤0.05). In conclusion, the ethanolic extract of Prosopis farcta L. fruits demonstrated promising antidiar...

Muhammad Farooq Bhatti1, Hafiz Muhammad Tahir1*, Aamir Ali1, Hooria Ashraf Khan1, Rabia Fajar Ali2, Ayesha Muzamil1, Fariha Munir3, Fatima Ijaz1, Rizwan Khurshid3

Sidra Hafeez1, Tayyaba Sanaullah2, Hafsa Naeem3, Mah Noor Hassan4, Muttalib5, Farhana Kausar6, Muhammad Salman Hameed7*, Muhammad Anayat Ullah8, Abdul Samad9, Memoona Bashir10 and Sadaf Shabbir11

...l primers ITS1/ITS4. The analysis of polygenetic of an amplified product revealed that black rot caused by D. bryoniae in pumpkin.

...

Jinwen Liu, Hong Li, Jinhua Zhang, Jianping Li and Xiujuan Yan*

...ne-way ANOVA and Pearson analysis was performed to process the data. The results showed that, the interactions between above- and below-ground herbivores had a negative effect on corn. Belowground herbivore grubs inhibited the aboveground herbivores of aphids, and aboveground herbivores prevented the increase of grubs, whereas plants regulated population change through interaction with above- and below-ground herbivores, thus reducing the survival rate of abov...

Ismail Demircioglu1, Yasin Demiraslan2, Barıs Can Güzel3*, Ali Koçyigit4 and Aysegül Demircioglu5

...were used for the dorsal analysis of cranium and heads of 8 adult Hamdani and 9 adult Awassi sheep were used for lateral analysis of cranium. In the dorsal and lateral comparison of the breeds, the first principal component (PC1) explained 41.905% and 39.078% of the total shape difference, respectively. When the procrustes coordinates were examined, it was found that the samples were both dorsally and laterally mostly simila...

Siswanto Imam Santoso*, Agus Setiadi, Maulina Syafa’ati Ningrum 

...oilers chicken. The data analysis method used was descriptive statistical analysis. Data were analyzed using the formula for production costs, total revenue, and profitability, BEP, payback period (PP), and R/C ratio. The statistical analysis used was the normality test and the one-sample t-test. The results of this study show that the broiler farm business in Kaliwungu District, Kudus Reg...

Khansa Jamil1, Muhammad Ramzan Khan1, Asad Jan2 and Ghulam Muhammad Ali1

...were identified by GC-MS analysis. The phytochemical screening showed that the methanolic extract of the whole plant was rich in alkaloids and tannins. Zones of inhibition around the bacterial colonies show the antibacterial activity of the plant. Extract of the targeted plant was effective against all test organisms Escherichia coli, Bacillus, Staphylococcus, and Pseudomonas that revealed methanolic extract of medicinal plant Caralluma possesses high antimicr...

Yu Song1*, Yueping Wei2 and Peng Wang3

...ies. Principal component analysis (PCA) revealed the similarity of samples at four growth stages was significantly low (P <0.05). The dominant taxa phyla for bacterial community were similar in different growth stages. The LefSe analysis indicated that 32 distinctly taxa that were abundant among four stages with a linear discriminant analysis (LDA) score higher than 3.5. The α-div...

Rudhy Gustiano1*, Iskandariah Iskandariah2, M.H. Fariduddin Ath-Thar1, Gleni H. Huwoyon4 and Deni Radona3

...e from sharing component analysis was Central Java population (73.3%), while the lowest was South Sumatra population (16.7%). The genetic relationship showed that the first cluster represented by populations from South Sumatra, Lampung, East and West Jawa. Even the hatching phase was the most critical phase in the early development of snakeskin gourami but the early development performance of fertilized eggs, embryos, and larvae showed no differences between o...

Wei Wan1, Xiao-Li Wang1, Shu-Juan Wang2, Qin Si2 and Li-Qing Duan1*

...yde. Pearson correlation analysis revealed a significant negative correlation between GST inhibition rate and number of antagonistic binary mixtures. In conclusion, our findings suggest that 2-ethylimidazole and cuminyl alcohol have high toxicity against P. sinica. L-carvone was the best synergist, and binary mixtures of l-carvone with dihydrocarvone, cuminaldehyde, cuminyl alcohol, d-carvone, and estragole showed potential as control agents against P. sinica....
Muhammad Furqan1*, Zulfiqar Ali1, Muhammad Mudassar Shahzad2, Rida Ahmad1, Faraz Akrim3, Imad-ul-Din Zangi4
...ble habitat mapping. The analysis for the contribution of environmental variables through Jackknife test showed that temperature was the prime environmental variable. Results revealed that out of total, 4388 km2, 406.03 km2 area was calculated to be highly suitable for kalij pheasant. Identification of hotspots and potential habitats for kalij pheasant can be considered as an important initiative to conserve the species.

...
Qingsen Ran1,2, Manjing Li3, Jiayin Han3, Lifang Wang3, Han Wang3,4, Shaobo Liu5* and Yanping Wang1*
...ough gene set enrichment analysis (GSEA) and Cox regression models. The K-M method was used to determine the difference of OS rate with high and low risk scores. The accuracy of risk scores were determined based on receiver operating characteristic (ROC) curve. Hierarchical analysis and Cox regression were performed to assess the correlation between CC risk score and clinical symptoms. Moreover, qPCR was used to verify the e...
Muhammad Javed Iqbal1, Farah Rauf Shakoori1*, Bushra Muneer2 and Abdul Rauf Shakoori3
...30Q and R173C. In-silico analysis of SNPs (PIK3CA, AKT1, MTOR and PTEN genes) confirmed that these mutations were responsible for cancer in respective patients under study.

...

Shehar Bano Bhatti1, Muhammad Irfan Malik2, Rabia Faryad Khan1, Mahmood Ul Hassan2, Muhammad Shahbaz Aslam3 and Zaigham Abbas1*

...was used for statistical analysis. HLA DRB1* 0701-02 and HLA DRB1* 1301-04 showed a positive association with atopic asthma having an odd’s ratio (OR) of 2.173 and 3.564 respectively. While HLA DRB1* 1101-04 and HLA DRB1* 1201-02 showed a negative association having OR of 0.4853 and 0.4299 respectively. HLA DRB1* 0701-02 had a positive association with family history (p = <0.0001*, OR= 3.641, 95% CI= 1.852-7.257) and female gender (p= 0.0003). Total I...

Mubashar Hussain*, Habiba Razaq, Muhammad Faheem Malik, Kiran Aftab, Jaweria Riaz and Somia Liaqat

...ties Percentage (SIMPER) analysis and diversity indices were compared among those three croplands. We found that Pheropsophus lissoderus was the most abundant and dominant species in all three study sites whereas Brachinus bilineatus and Galerita lecontei were recorded only in tehsil Phalia, Pheropsophus verticalis, and Dioryche subrecta in Malakwal. Pheropsophus was the most abundant and dominant genus representing six species in all three sites. Overall Shan...
Moustafa A. Zaghloul1*, Mohamed F. Azooz1, Saleh E. Ali1*, Heba M. Soliman1, Maha M. Sayed1, Mohamed H. Kafafy2 and Alaa R. Morsy1
...uencing and phylogenetic analysis of GPCR, RPO30, P32 and EEV glycoprotein genes of Lumpy Skin Disease Virus (LSDV) recent isolates in Egypt, as well as to use artificial intelligence to predict the immunogenic landscape of circulating lumpy skin disease in the Egyptian cattle dairy sector, which will lead to universal blueprints for multiepitope lumpy skin disease vaccine designs. A total of 40 skin nodule samples were collected from clinically affected cattl...
Akram A. Salama1, Moustafa A. Zaghloul2*, Ahmed A. Zaghawa1, Mohamed A. Nayel1, Ahmed M. Elsafey1, Mohamed F. Azooz2, Hanem S. Harb1 and Walid S. Mousa1
...was 94.1%. The alignment analysis revealed a distinct 27-base deletion in the vaccine strains. Our results can be used to better understand the epidemiology of LSDV in Egypt and to build an effective disease control programme. Furthermore, our findings emphasize the importance of continuous surveillance and characterization of circulating LSDV strains, as well as the need to improve the DIVA strategy for distinguishing the vaccine strains from field viruses.
Hanaa H.A. Gomaa1*, Dalia Y.A. Amin1, Mona A. Ismail1, Basma Hamdy2, Khaled A. El-Dougdoug3
...me and ultrathin section analysis revealed that improved phytosanitary of cell and tissues of FLV infected fig plants treated with ChNPs and BM, as well as improved shoot length, leaf area and dry weight. Biochemical markers as indicators for systemic acquired resistance were significantly increased including total proteins, salicylic acid, phenol, proline, Peroxidase, polyphenol oxidase and superoxide dismutase activities Nanochitosan and biomagic were treate...

Zhimin Wu1,2, Yaping Yang1, Arshad Zahoor1, Aftab Shaukat1 and Ganzhen Deng1,2*

...elected with correlation analysis and single factor analysis of variance. The posterior lung field angles and the thoracic indexes showed no correlation in both healthy group and unhealthy group (r<0.3). The posterior lung field angles and the thoracic indexes between healthy with unhealthy dogs showed low correlation (r<0.5). The thoracic indexes between both groups were no significantly different (p>0.05). Only ve...

Usama Mahalel1, Barakat M. Alrashdi1, Ibrahim Abdel-Farid1, Sabry El-Naggar2, Mohamed Hassan3, Hassan Elgebaly1 and Diaa Massoud1,4*

...nation and liver enzymes analysis. Immunohistochemical investigation showed that the administration of M. forsskalii extract at a low dose downregulated the expression pattern of P53 and upregulated the expression of Bcl-2 in liver tissue. In conclusion, our findings indicated that the methanolic extract of M. forsskalii at a low dose (100 mg/kg BW) attenuated the hepatotoxicity induced by CCl4 through upregulation of Bcl-2 expression and restoring liver enzym...

Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1

...sion electron microscopy analysis classified SEPL01 as a Siphovirus and SEPL13 and SEPL20 as Myoviruses. In liquid culture, phage virulence activity was rapid and high at a high (100) multiplicity of infection with phage SEPL01 depicting the best activity among all. Compared to the solophage suspensions at high MOI, the phage cocktail comprising all three phages did not let the bacteria regrow until 24 h overcoming bacterial resistance. All the three bacteriop...

Taidong Wang1, Xiaowei Huang1, Jian Huang2, Guangfu Lv3 and Zhe Lin1*

...2 software, and topology analysis was performed on the PPI network. KEGG signaling pathways were analyzed using DAVID database, and molecular docking verification was performed on the core targets after topology analysis. Subsequently, 24 rats were randomly divided into control group, model group, wild high-dose group and wild low-dose group, 6 rats in each group. Results of in vivo experiments showed that high-dose group (P...

Rasema Majeed, Alaa Kamil Mahmood* 

...d diabetes. histological analysis was assessed. The results showed that first group (Control negative) administered distilled water showed no abnormal lesion, while second group (Control positive) is diabetic without treatment showed destruction and necrosis of islet cells, congestion and edema between their acini. Third group was administered 81.7 mg/kg BW of ginger ethanolic extract orally for 45 days showed normal section of islet cells shows moderate necro...

Lijiao Wang1, Haibin Chen2*, Hongyan Yu1, Zexian Fu3 and Jianjun Zhao2*

Suneela, Sajida Mushtaq*, Sadia Maalik, Asma Waheed Qureshi and Moazama Batool
...bundance. By statistical analysis  significant difference (p-value< 0.005) was found in presence of diversity in different months of sampling. Shannon index was maximum in the month of May (H’=2.819). Canonical Corresponding Analysis (CCA) determines the impact of different environmental factors on presence of different species.
...
Budi Utomo1*, Amaq Fadholly2,3, Endang Tri Margawati2, Alek Ibrahim2, Ristaqul Husna Belgania1, Muhammad Fajar Amrullah1, Rimayanti1 and Hermin Ratnani1
... SRY gene is used in the analysis of genetic diversity for sex determination based on the paternal pathway (Y chromosome). The DNA samples used for this study were 10 frozen straw of Limousin cattle and 10 samples of Madrasin cattle. The method used in this research was a descriptive analysis by duplex polymerase chain reaction and analyzing the results of the SRY gene sequencing of limousine cattle and Madrasin cattle. In t...
Sudhan Chandran1*, GB Sreekanth2, Geetanjali Deshmukhe1 and  Ashok Kumar Jaiswar1
... data, linear regression analysis was performed to calculate a and b values for two fish species to establish length-weight relationships with respective coefficient of correlation and the 95% confidence intervals. A new maximum total length was recorded for two creek associated mangrove fishes, viz., Escualosa thoracata (10.1 cm) and Bregmaceros mcclellandi (10.5 cm).

...

Rahmat Elahi1, Gulnaz Parveen1*, Nazara1, Faryal Ali2, Nain Tara3 and Saba Iqbal1

... significant difference) analysis showed interesting results. By targeting and treating the maize seeds with castor oil, it could potentially help mitigate the adverse effects of these fungi and contribute to a healthier and more productive crop. This study aimed to investigate the effectiveness of castor oil in managing seed-borne diseases caused by these fungi. This is a promising direction for future research and application in agricultural practices to ens...

M. Azalou1,3*, C. C. Kpomasse1, A. S. Assani3,4, I. T. Alkoiret3,4, Wéré Pitala1,2  

... Multiple correspondence analysis (MCA) and ascending hierarchical clustering (AHC) identified three groups of goose farmers. Group 1 was composed of primary and secondary level goose farmers whose main activity was trade (56.1%); group 2 was composed of secondary level herders whose main activity was agriculture (59.38%); and group 3 included public servants (27.59%) with university-level education. As for the feeding of the geese, Group 3 fed more feed than ...

Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2 

...ts were revealed by FTIR analysis of CPNPs. The SiO2NPs’ FTIR spectrum shows two vibrations that can be attributed to SiO2’s Si—O—Si and Si—O vibrations, respectively. Thirty-two Female rats that had reached adult were randomly split into four groups: Control; T1: received oral 200 mg/kg of SiO2 - NPs were gavaged orally to this group of rats. T2: received oral 100 mg/kg of CPNPs and Sio2NPs. T3: received oral 100 mg/kg of ...
Johan Sukweenadhi1*, Stefan Pratama Chandra1, Finna Setiawan2, Christina Avanti2, Kartini Kartini2, Arief Koeswanto3 and Deok-Chun Yang4
.... Based on a statistical analysis of the average wound area, epidermal reconstruction, and TIME-H scores, significant differences began to occur from day 12 to day 14. The average wound area, epidermal reconstruction, and TIME-H scores in the diabetic mice group that were given a dermal patch formulated with silver nanoparticles synthesized using Plantago major L. extract were not significantly different from the normal group and the ACA and ACA Ag grou...

Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni

...med. In the phylogenetic analysis, the three sequences and 80 sequences of HCV different genotypes from GenBank were used. Nucleotide variations were found in 7 (3.14%) of the 223 nucleotide positions analyzed. The variations were observed in the nucleotides (74, 92, 112, 113, 133, 172, and 180). Nucleotide insertion was seen in one sequence in the nucleotide position of 212. Root neighbor-joining (NJ) tree revealed that the three HCV samples could be classifi...

Yousifl ,3 , Ausama A. and Al-Naeem , Abdelmohsen A.

...btained with restriction analysis and underlines the need for a review of some of the vaccines used to control CMLV.

...

El-Bramawyl , M . A. S. A. and El-Beshehy2, E. K. F.'

...on's plants. Our results analysis confirms that the Fl 's was completely resistant and the F2's was segregated a clear to been 3 resistant: 1 susceptible, while the F3's families confirmed the F2's families segregation, which suggests as a dominant character controlled by a single locus. This resistance character in faba bean considering has a good potencial for direct use in commercial faba bean breeding or for transfer to other faba bean genotypes.

...

Elbeshehy, E. K.F. and Sallaml, A.A.A.

...erological and molecular analysis. The isolated virus gave positive reaction with CMV antiserum but not with antibodies of WW and SqMV using DAS-ELISA. CMV was able to infect different host plant species including squash, pumpkin, pepper, bean, Chenopodium amaranticolor and cowpea showing foliar symptoms of mosaic, deformations and necrotic and chlorotic ring spots, that resemble those induced by CMV. SDS-PAGE test showed various distinguishable sole novel pro...
Nassarl , Entsar A.; El-Dougdoug , Kh. A.; Osmanl , M.E; Dawoud3, Rehab
A. and Kinawy l , Aliaa H.*
...serological and sequence analysis of the coat protein gene demonstrated that the virus represents an isolate of the Tobamoviridae Family. The isolated virus was nominated as TMV Chrysanthemum Egyptian isolate (TMV-Ch-EG). This virus isolate caused severe disease symptoms in Chrysanthemum plants with mosaic, mottling and flower discoloration. The virus was purified biologically using serial transfer of the single local lesion technique on Nicotiana gultinosa. T...

Soliman*, Ahmed M.; Mahmoud**, Sabry Y. M. and Dawood*, Rehab A.

...in GenBank. The sequence analysis showed that; nucleotide sequence of OYDV-EG [Egyptian isolate (HM473189)] had 82-96% similarity compared with ten reported OYDV isolates. Thus, a method of identification and detection by RT-PCR of OYDV was established. This study also aimed to obtain OYDV-free plants from infected garlic plants using cloves subjected to electrotherapy, thermotherapy, chemotherapy or meristematic dissection followed by in vitro culture. A comb...

Sharawil , S.S.A. and Abd El-Rahim2, I.H.A.•

...tein gene and phylogenic analysis properties, by matching with other reference world recorded isolates. The gene sequenced a 322 nucleotide cDNA fragment of the fusion protein gene was obtained. The isolates showed unique phylogenic analysis had not previously been identified internationally and defined as Egypt 2009. In addition; by using the obtained sequences generated from this gene coding protein; Egyptian PPRVs were gr...

Zein Salwa N; Abd El-khalik, Samaa; Khatab  , Eman A.A.H and Azzam4,Clara R.

...o TMV-S. The statistical analysis indicated significant differences among irradiation doses on plant height; all used doses increased plant height comparing with the control. Inoculation with TMV-S caused decreases in plant height over all other factors. Number of leaves differed significantly according to the used cultivars, gamma ray doses and T&W-S. The highest number of leaves were found during M2 generation, The differences in head diameter between cu...

*El-Helaly, Sahar H.; ** Ahmed, Amal A.; * Awad, M.A. and ** Soliman, A.M.'

...as confirmed by sequence analysis of their coat protein (CP) gene. The sequence of the coat protein gene (CP) of AMV was determined from cDNA clones. The CP gene was cloned into pGEM-T Easy vector, and transformed into Escherichia coli (E. coli) strain DH5a. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The analysis<...

Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi

...s of nucleotide sequence analysis of I I detected viruses confirm the existence and circulation of the H5Nl in Sharkia governorate along the period of study and no H7 viruses were detected. The serum samples collected from different farms were divided into 4 groups based on the date of collection. HI results showed that in the first group (samples collected between December 2006 to March2007) 43% of the tested samples were of HI titers of 6 log 2 oc more which...

Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty

...nbsp;  phylogenetic analysis of haemagglutinin (HA) and neuraminidase (NA). genes of 2006 strains revealed that these isolates are located in Euro•Asio-African lineage which contains Al H5Nl strains from Egypt, Turkey, Romania, Iraq, Mongolia, Iran, Korea and Nigeria and differed from those strains isolated in South-Eastern of Asia which contains Cambodia, Thailand, and Viet Nam Al H5Nl strains. The phylogenetic analysis

El-Tarabili M. M., El-Shahidy M. S., Rifaat M. M., Kania S. A. and Abdelwahab Shahira A.

...p. Nucleotide sequencing analysis of selected cases from cattle and deer showed that the similarities among the detected positive samples and published one ranged between 98-100% for both. The high prevalence of MCF in cattle and deer shows the significant economic burden of the disease in these animal species.

...

Hassanein, S.A.*; Ibrahim, A.K**; Mervat, M.M.* and   Nahed,K.A.*

...d Polymorphic DNA (RAPD) analysis. Antigenic comparison using VN test and their effect on MDBK cell culture detected no difference, thus demonstrating the close antigenic relationship between the examined herpesviruses. Using the restriction endonucleases BamHI and EcoRI revealed no difference between the examined five viruses. But by the use of Hindlll restriction endonuclease and RAPD, fingerprint patterns were capable of differentiating the analyzed five vi...
Ashraf M. Metwally*, Ausama A. Yousif*k , Iman B. Walaa A.   Attia M. Samy * , and Ismail M. Reda *
 
...ed samples. Phylogenetic analysis of the sequenced PCR product and deduced amino acid sequences of IBDV Giza2008 VP2 demonstrated the continued circulation of very virulent IBDV (vvIBDV). The mutations reported in Giza2008 demonstrate that Egyptian field viruses are isolating from their European ancestors.  Some of the aa mutations have lead to a change in some of the exposed regions of the viral protein. Our findings explain the continued presence of vvI...

A-New-Whitefly-Transmitted-Geminivirus

... subjected to biological analysis, the viral causal agent vector of the two types of symptoms was found to be Bemisia tabaci (Gennadius). Based on diagnostic host species and back inoculation assay two different types of symptoms were found. The first consists of identical Tomato yellow leaf curl virus (TYLCV) symptoms such as severe leaf chlorosis and distortion on tomato plants. named TYLCV-Giza isolate, and the other one showed yellow mosaic symptoms on tom...

Seham A. El-Zeedy1, Dalia A. Abd El-Moaty1, H. A. Hussein2 and M. A. Shalaby2

...as confirmed by sequence analysis or these RT-PCR products of the hyper variable region and the deduced a.a sequence 183-360, has the highest identity on both nucleotide and amino acid levels 99.4% and 98.8% to the classical strains Cu-l, D78, and PBG-98, while Giza/2000 has the highest identity 98.3-98.1% on nucleotide level and 98.3% on a.a level to the vvIBDV. The multiple alignment of the two Egyptian isolates with different IBDV serotype I strains showed ...

M.S. Wassel1; Elham A. El-Ebiary1; Soliman, Y.A.l and El-Sayed, M.M.2

...nimals using its protein analysis and Western blotting.

...

Huda M. Hamid1, Fadia W. Al-Azawi2* and Zaid F. Makki3

...ation. In this study, an analysis of hydrology characteristics of Iraqi dams has been presented which include DAM flow direction, mesh flow, agricultural area improvement, waterway division, and basin determination. These characteristics provide valuable insights into country’s water resources management, including the optimization of water use for different purposes and the strategies’ development to deal with water scarcity and drought. The

Rawaa Saladdin Jumaa

...Iraq to look at P4b gene analysis of pigeon poxvirus virus in locally infected pigeons with crust lesions in the eye and nostril cere. The DNA extracted from the collected samples was amplified using conventional PCR by amplification of the P4b gene. The positive samples had been inoculated on chorioallantoic membrane (CAM). Thickening of CAM were seen in the first passage, whereas pock lesion at the second (2nd) and third (3rd) passages. The chicken embryo fi...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

...t antigen, bioinformatic analysis of the primary structure of T. evansi GM6 antigen from several regions was carried out. The protein sequence was reverse translated to the nucleotide sequence, after which the gene was synthesized using solid-phase method. The gene fragment, encoding the GM6 protein, was inserted or cloned into the pET28 expression vector after synthesis. The obtained sequences were checked by sequencing for correspondence to the matrix molecu...

Zhengfei Wang1*, Chenchen Shen1,2, Yiping Zhang1, Dan Tang1,3, Yaqi Luo1, Yaotong Zhai1, Yayun Guan1, Yue Wang1 and Xinyu Wang1

Syed Majid Rasheed1*, Tauseef Ali1, Saad Jan1, Faiz ur Rehman2, Muhammad Ali Shah3, Zahid Hussain1 and Abdul Waheed4

...bing ability and genetic analysis in Okra. Twenty-eight okra genotypes (5x5 diallel crosses) and checks were screened for high yield and early maturity in a randomized complete block design. Highly significant differences were observed for most traits, except stem diameter and marketable fruit diameter. Among genotypes, G10 flowered earliest (41.5 days), G22 had longest internodes (3.59cm), G16 had heaviest fruits (9.65g), and G14 had longest and thickest mark...
Raed M. Al-Atiyat1,2*, Gamaleldin M. Suliman3, Khaled Abu-Alruz4, Firas Al-Zyoud5, Amer Mamkagh6, Ahmed M. El-Waziry7, Abdullah N. Al-Owaimer8 and Rifat Ullah Khan9
...e multivariate technique analysis was utilized for assigning and discriminating three Saudi sheep breeds, Naemi, Najdi and Hari based on live body and carcass traits. The traits were body height at wither and rump, body length, heart girth, body depth, head length, ear length, body weight at slaughtering, empty body, dressing percentage, hot carcass, cold carcass, and head weight which had a significant effect on breed. The phenotypic associations between the ...

Nejra Dučić1, Senad Kovačević1, Faruk Tandir1,2, Rizah Avdić2, Ermin Šaljić3, Anel Vejzović2, Nedžad Hadžiomerović2* 

...r cats. The morphometric analysis was performed on 20 parameters. Our study revealed smaller values (6.25 ± 0.31 mm) for the distance from the infraorbital canal to the root of premolar compared to Persian and Australian domestic cats. The present study provided a valuable information about the location of the cranial nerves on the skull and mandible of the domestic shorthair cats, which can be useful during dental procedures.  

...

Muhammad Wasif Gulzar* and Jawad Hussain

...trol, and a risk-benefit analysis. We think AI tools should only be used in clinical settings once they have undergone thorough validation. Once that is done, we think they should be continuously checked in clinical settings. Furthermore, it is imperative that veterinary practitioners receive precise instructions and guidelines for the best possible use of these tools. 

...
Zeynep Karapinar1* and Mehmet Özkan Timurkan2
...otyping and phylogenetic analysis of the positive samples were performed. It was observed that five of the FPLV positive samples obtained were in the G3, one sample was in the G1, and one sample was in the CPV-2b group. FPV was investigated for the first time in Balıkesir province and the data of the feline parvovirus sequence was defined to Genbank. Current phylogenetic information about the FPV in Turkey was obtained in the present study.

...

Sarah G. Yousef1*, Ahmed Shehta2, Hend M. El Damaty1 and Hussein A. Elsheikh3

...uencing and phylogenetic analysis revealed that our strains (ON816021 and ON816022) were 100% identical showed complete identity with MAP-C (M. paratuberculosis of cattle origin) as Japanese (CP066812) and German strain (CP022105). Overall, the findings highlight the potential of the IS900 gene-based molecular technique for MAP detection in small dairy cattle farms.

...

Sajjad Khan*, Naila Chand, Abdul Hafeez and Nazir Ahmad

...ental trial. Statistical analysis was performed by SPSS following CRD design and data was expressed as means along with SEM after performing Tukey’s test. Results indicated significantly high feed intake, body weight gain, improved (P<0.05) FCR, livability and EPEF in group D followed by C, B and E. Same pattern of improved weight of heart, liver, gizzard, pancreas, bursa, spleen and thymus was recorded for the groups studied. Significantly higher (P&...
Asad Ullah1, Hayat Ullah2, Muhammad Lateef3, Imrana Niaz Sultan4, Afrasiab Khan Tareen4 and Muhammad Waseem Khan4,5*
...TB suspects in the final analysis. The results of GeneXpert were compared with Mycobacteria Growth Indicator Tube (MGIT) and Ziehl-Neelsen (ZN) staining. In comparison to MGIT and ZN staining the sensitivity of GeneXpert with 95 % confidence interval (CI) was (99.7 %, CI 0.98-0.99) and (95.1 %, CI 0.92-0.97) respectively. The positive and negative predictive values with 95 % CI were (97.1 %, CI 0.94-0.98) and (99.7 %, CI 0.98-0.99) when results of GeneXpert we...

Lingang Dai1,2,3, Xiang Chen1,2,3, Jiajin Huang1,2,3, Jiali Xu1,2,3, Meimei Xiao1,2,3, Jiajing Chen1,2,3 and Yong Ruan1,2,3*

...P<0.01). Polymorphism analysis of the PGAM2 gene showed that there were polymorphic loci in the 3’UTR (2435A→G), and there were significant (P<0.01) or highly significant (P<0.05) differences in some traits between the GG and AG or AA genotypes. Conclusion: The production performance of male goats is better than that of female goats in F3 generation hybrid goats, and the slaughtering performance of F3 generation hybrid goats was significant...

Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4 

...e analysed using one-way analysis of variance (ANOVA). A Descriptive Statistical test used to analyze the proportion of sperms. The results showed that different concentrations of sexing media showed no significant difference (P>0.05) on motility, viability, abnormality, membrane integrity, acrosome integrity, and movement patterns of spermatozoa in treatment between T1, T2 and T3 treatments both in the upper and lower fraction. In the movement pattern of s...

Ahmed M. Manthoor, Ali H. Saliem* 

...aratus and phytochemical analysis of extract. While in the second step, the antibacterial effects of the extract as compared with ciprofloxacin, as well as zone of inhibition, minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), by using 4, 8, 16, 32, 64 and 128 mg/ml concentration of the extract, while 1.562, 3.125, 6.25, 12.5, 25, 50, 100, 200, 400, 800 µg/ml concentration of Ciprofloxacin, on the other hand study me...
Geeta Moolchandani1, Jaiperkash Moolchandani2*, Nasima Iqbal3
Syed Mohsin Turab4, Muhammad Kashif5 and Lubna Farooq6
...ting factors. Stratified analysis showed that over all neonates of female gender were associated with having more frequency of HIE. Maternal anemia was found to be significantly associated with the occurrence of HIE. The frequency of HIE risk increased with increasing maternal age. Lowest the serum pH level, highest was the frequency of HIE risk factors. Prolonged 2nd stage of labor was significantly associated with lower neonatal weight, overall, very low and...

Taleeha Roheen1, Shagufta Kamal1*, Shazia Anwer Bukhari1, Ghulam Mustafa1 and Saima Rehman2

...udied using phylogenetic analysis. Morphological and biochemical analysis showing the presence of different species of novel methanogenic archaea and sMMO-producing methanotrophic bacteria in rice paddy soil samples. The evolutionary analysis indicated a phylogram with five monophyletic groups (MPG-I to MPG-V). Results revealed that three isolated species (Sphingomonas sp. MG-1, Sphingomon...

Skeikh Mustafizur Rahman1*, Mst. Shirin Sharmin Khan1, Yousef Ahmed Alkhamis2,3, Roshmon Thomas Mathew2, Md. Moshiur Rahman1, Mohammed Monirul Islam4, Md. Asadujjaman5 and Md. Golam Sarower1

...mparable. The regression analysis revealed that fish price increased with the increase of their protein contents. The study has provided the nutritional information of these selected fishes which are mostly consumed by the general coastal inhabitants in Bangladesh.

...
Nael Abutaha, Fahd A. AL-Mekhlafi*, Khalid Elfaki Ibrahim, 
Mohammed S. Al-Khalifa and Mohamed A. Wadaan
...fied using phytochemical analysis and gas chromatography-mass spectrometry (GC-MS). The cytotoxic effect was also evaluated using normal human umbilical endothelial cells (HUV-EC). The larvicidal assay was performed on the third instar stage as recommended by WHO. The phytochemical analysis showed the presence of alkaloids, sterols, and phenolic compounds. The GC-MS analysis revealed the p...

Seyed Ahmed Reza Hashemi1, Teymour Aminrad1, Asadullah Ali Muhammad2*, Mastooreh Doustdar3 and Parastoo Mohebi Derakhsh3

...rtificial neural network analysis indicated that two parameters, phosphate and nitrate, had the greatest impact on sea cucumber biomass. To implement a maximum sustainable yield method for this fishery in Iran.

...

Tinda Afriani1*, Jaswandi1, Yurnalis1, Putri Oktavially1, I Made Merdana2 

...rmone variations through analysis of the exon 5 gene. This study aimed to identify the characteristics of qualitative and quantitative traits and growth hormone patterns in crossbreeds between Pesisir and Friesian Holstein (FH) cattle. A total of 60 crossbreeds of Pesisir and FH cattle were used. The method employed involved a molecular analysis of growth hormone in the exon 5 gene using Polymerase Chain Reaction (PCR), alon...
Syed Tayyab ul Mazhar1, Hasnain Khan1, Uzma Afzal1*, Shazia Usmani1 and Tariq Mahmood2
... data science, sentiment analysis, natural language processing, psychology and others.
...
Syed Tayyab ul Mazhar1, Hasnain Khan1, Uzma Afzal1*, Shazia Usmani1 and Tariq Mahmood2
... data science, sentiment analysis, natural language processing, psychology and others.
...

Alveena Izhar1*, Basit Ullah2, Rabia Asghar1, Aqeel Ahmad1 and Hassam Bin Mujahid1

...ubplots. The statistical analysis of the experiment showed that various phosphorus levels enhanced all the quantitative parameters of the radish crop. Maximum, plant height (65 cm), number of pods plant-1 (187), pod length (7.9 cm), pod diameter (3.70 mm), seed pod-1 (8), 1000 seed weight (22.6 g), seed yield plot-1 (25.8 g), seed yield (170.5 kg ha-1) and seed germination viability (97.8) with less number of days to 50% flowering (32) were noted in the plants...

Ram Prasad Ghimire

.... The study included the analysis of fodders in the laboratory for nutrient composition, the feeding experiment determining fodder intake and weight gain monitoring in the goats, and a subsequent in-vivo experiment determining the apparent digestibility of fodder nutrients of those browse species. Both of the experiments were conducted in Randomized Complete Block Design (RCBD) with six treatments and five replications on native hill goats of Nepal. The fodder...

Theresia Nur Indah Koni*, Yeria Banoet, Welhelmina Wahon, Cytske Sabuna, Melkianus Dedimus Same Randu, Yanse Yane Rumlaklak, Tri Anggarini Yuniwati Foenay

...n were analyzed by using analysis of variance and continued with Duncan’s multiple range test. The results of this study indicated that the anaerobic fermentation process for 21 days increased the crude protein content, and decreased the crude fiber and tannins of banana peels. The level of fermented banana peels had a significant effect (P<0.05) on body weight gain, feed intake but had no significant effect (P>0.05) on feed conversion. Body weight...

Aimal Zeb1, Abdur Rauf1*, Nabila Bano2, Muhammad Qayash3, Muhammad Yasin4, Ikramullah Khan1, Syed Abidullah1, Wisal Khan5, Muhammad Asmat Ullah2, Abdul Ghaffar Khan6 and Samrin Gul

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

...ed with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in 10 replicated experiments. The results showed that sperm motility, viability, and plasma membrane integrity were significantly higher (P<0.01) when 2-3 mM BHT...

Henny Leondro1*, Peni Wahyu Prihandini2, Yuli Arif Tribudi3, Aju Tjatur Nugroho Krisnaningsih1, Dimas Pratidina Puriastuti Hadiani1, Agung Kusuma Wijaya4, Dyah Tuwi Ramsiati2, Mochammad Chanafi2, Wahyuni Indah Wulansari2

...aceous legume, Proximate analysis, Biomass production, Nutritional value, Animal feed
...
Yongtao Xu1, Dandan Wang1, Xiaolong Hu1, Minling Li1, Ming Tang1, Wuhua Liu2, Jianwen Zhan2 and Weiwei Zhang1*
...cies left in China. Diet analysis is one of the core contents in studying animal habitat requirements, and a study of their diet could provide valuable reference for species conservation and management. The metabarcoding trnL regions of the chloroplast genome from 30 fecal samples of Taohongling sika deer were amplified with universal primers and sequenced by high-throughput. We found that 28 out of 30 valid samples had a total of 677,856 valid amplified seque...

Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Vincentia Trisna Yoelinda1, Sarmin1, Yuda Heru Fibrianto1, Pudji Astuti1

...homogeneity, followed by analysis through the T-test method. The results indicated an increment of testosterone and corticosterone hormones among experienced Stone Magpie birds after the contest; however, these changes did not reach statistical significance. The mean values, both before and after the contests, of testosterone levels in the amateur group did not show a significant difference. However, corticosterone levels in this group showed significant diffe...

Pramudya Andiana*, Moch. Geerhan Miraja Syahdan, Khothibul Umam Al Awwaly, Abdul Manab

...e analyzed using one-way analysis of variance followed by Duncan’s multiple range test. The results showed that different concentrations of the combination of bromelain and papain had a significant effect (P<0.01) on pH, soluble protein concentration, peptide concentration, degree of hydrolysis, IC50 of DPPH, reducing power, iron and copper chelating ability, and α-amylase inhibitory activity. CHP1 showed the best bioactivity with a DPPH IC50 va...

Abdeljabbar Nassiri1, Mouloud Lamtai1*, Inssaf Berkiks1,2, Hajar Benmhammed1, Sidi Mohamed Coulibaly1,3, Miloud Chakit1, Laila Ibouzine-dine1, Abdelhalem Mesfioui1, Aboubaker El-Hessni1

... the PFC for biochemical analysis, specifically measuring nitric oxide and lipid peroxidation. The results indicate that, in both genders, MD generates an anxiogenic and depressive effect compared to the control group, which are linked to an increased OS in the PFC of rats, with greater impact during adolescence and adulthood. Interestingly, most measures showed sex differences in responses, particularly in the MD group. Our findings suggest that MD causes sho...

Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2

...esults from the PCR-DGGE analysis showed differences in bacterial diversity, density, and banding patterns along the gut of buffaloes. Higher bacterial diversity, density, and banding patterns were observed in the foregut and hindgut as compared with the midgut. PCR-DGGE fingerprints further revealed that samples from the foregut and hindgut shared similar banding patterns. Although the midgut was the least diverse segment, there were unique bands that can be ...

Qamer J. Jadoaa, Raffal A. Omar*

...ne healing, Histological analysis, Intraosseous injection
...

Frennada Lu’fatul Azizah1, Osfar Sjofjan2*, Eko Widodo2

...ected and analyzed using analysis of variance (ANOVA). The experimental data on the use of BSF oil as a feed additive yielded significantly different results (p < 0.05) for the feed conversion ratio (FCR) and performance index (IP) of broiler chickens. In summary, the use of different BSF oils has the potential to be employed as a natural feed additive for broiler chickens.
 
Keywords | Alternative growth promoters, Black soldie...

Yude M Yusuf1,2, Rudy Priyanto1*, Jakaria1

...n=24) were collected for analysis. The effects of different genotypes were tested using one-way variance analysis, followed by Duncan’s multiple range test. The parameters analyzed were body weight (BW), chest circumference (CC), body length (BL), and shoulder height (SH) at birth, weaning (205 days), and yearling (365 days). The principal component analysis was performed to find the...

Babi Kyi Soe1*, Toe Win Naing2, Su Lai Yee Mon1, Nay Chi Nway3, Hiroshi Sato4

...red by blood biochemical analysis, whereas total protein and albumin were low. To our best, this study is the first molecular evidence for the presence of Anaplasma marginale infection in cattle in Myanmar, with a sequence similarity range between 98.8 and 100%. By understanding one of the major tick-borne pathogens (TBPs) in Myanmar, possible control measures might be implemented not only to minimize the transmission but also to increase the farm productivity...

Irfan Ullah1, Asad Ullah2*, Tahira Tayyeb1, Rafiq Ullah1, Muhammad Hanif1, Faiza Khan3, Imad Khan2, Raheela Taj4, Fatima Syed4, Shumaila Gul5, Muhammad Sadeeq6, Muneeb Islam7, Arsalan Khan8 and Khudija Ghani9

...es at 1000 rpm for serum analysis and anticoagulant added tube for whole blood. The serum was stored at -20°C until further analysis. Biochemical parameters were measured through commercial kits. The result of the current study showed that the values of Alanine transaminase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP) were higher significantly (P≤0.05) in negative control group (B) as compare...

Eluzia A. Barus*, Khansa N. Salsabilah, Achmad Firman, Linda Herlina

... 2,187,762,256. The SWOT analysis results show that Camara Farm’s business development strategies should focus on building expansion, better management and price-contractor, higher quality breed, feed, and human resources, business diversification, and technological advancement.   Overall, the Camara broiler farm business is profitable in the short term and has potential to provide even higher benefits in a long run.
 
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Munifah Siti Amira Yusuf1, Norsida Man2*, Nur Bahiah Mohamed Haris1, Ismi Arif Ismail3, Siaw Shin Yee1 and Tengku Halimatun Sa’adiah T Abu Bakar1,4 

...alysed using the PLS-SEM analysis method with the help of SMART-PLS software version 3.0. Findings revealed a positive association between all CIPP factors and Effectiveness. These findings showed that the DOA should prioritise process and product as the agriculture extension provider (stakeholders). This study suggests that policymakers should improve the implementation of urban agriculture programs in context, input, process, and product at a community level...

Shahid Iqbal1, Arshad Khan, Ali Hazrat1*, Gul Rahim, Mohammad Ihsan1, Umar Zad Gul1, Maryam Bibi1, Khadija Bibi1 and Muhammad Mukhtiar2

... Coefficient correlation analysis was computed for all the quantitative traits, where a positive correlation was recorded for plant height (0.37), leaf length (0.18), and seed per plant (0.127). Biomass per plant shows a positive correlation with leaf length (0.118), leaf width (0.27), and seed per plant (0.22) whereas a negative correlation was found in petiole length (-0.044), plant height (-0.002), pod length (-0.59) and pod per plant (-0.99). Principal com...
Sijie Jian1,2,3,4, Wei Sun1,3, Jia Chao1,2, Na Rong1,4, Xiang Liu1,2,3,4* and Chen Chen1,2,3,4*
...ines. Slp bioinformatics analysis showed that anti-Slp serum might provide cross-protection to resist bacterial infection in fish. Slp was obtained by molecular cloning, expression and purification, and the expression conditions were optimized. In mice immunized with Slp, the immune-related factors of LZM and AKP were enhanced (p < 0.05), and a specific antiserum titer (1: 3200) was obtained that had immune recognition effects for A. hydrophila and P. fluor...

Perinçek Seçkinozan Seker

... the evolutionary dating analysis, the divergence of C. capreolus populations in Europe started at the beginning of the Pleistocene (approximately 2.72 mya). This analysis also demonstrated that the divergence of Turkish roe deer began at approximately 0.58 mya. Given the existence of, shared haplotypes, the relative positions of Turkish roe deer haplotypes in the topologies of the ML and BI phylogenetic trees and evolutiona...
Fen Wang1,2, Lin Zuo1,2, Xiang Zhou1,2, Zhu Chen1,2, Xiao Na Xu1,2, Suo Fei Ji1,2, Guan Jun Hou1,2, Cheng Jun Zhu2, Ye Zhang3, You Feng Su4, Gendong Jin5, Jia Jia Wang1,2, Yuan Gao6, Guang Tong Song1,2* and Ye Lin Jiang1,2*
...omposition. Our nutrient analysis results indicated that both types were high-quality aquatic products with high protein and low fat contents. The amino acid contents in the muscle of type A were significantly higher than those of type B (P < 0.05), but no significant differences were observed in the calipash. Additionally, the amino acid score (AAS) in the muscle of type A was >1, which was significantly higher than that of type B (P<0.05). The conte...
Aatif Masood Ahmad Khan1, Masood Rabbani1*, Arfan Ahmad1, Muhammad Wasim2 and Sohail Raza1
...utine, culture-based PCR analysis and isolated Av. paragallinarum can be grown in BHI/SN to achieve maximum biomass, a trait required for vaccine production, using brain heart infusion broth media.

...

Rogia SA Gomez*, Said H Mbaga 

...to a principal component analysis (PCA), enabling three groups of farmers to be distinguished. The productivity of each group was assessed based on the economic information collected from the farmers. Overall, the results showed that the number of birds per flock was less than 1000 on farms ranging in size from 81.2 to 206.3m2. Farmers used more family labour (89.7%) to raise broilers. The grouping of respondents showed that 59% of them (group 1) were beginner...

Fazal Ur Rehman1*, Saeeda Fatehullah1, Ayaz Qadir1, Suleman Ahmad2, Siddique Usman2, Muhammad Asif2, Mukhtiar Ahmed1, Sunaina Rafi3, Muhammad Mohsin2, Ayesha Amanullah4, Faisal Shahzad2, Hasnain Raza2 

Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*

... for immunohistochemical analysis. The findings revealed that CCoV vaccine induction in the treatment group resulted in duodenal mucosa haemorrhage, necrosis, and severe inflammation. Conversely, in the group treated with Lactobacillus acidophilus, tissue damage was inhibited, demonstrating the protective effect of the probiotic. Lactobacillus acidophilus effectively mitigated lung, liver, and duodenal damage by reducing the expressions of TNF-α and IL-1...

Moazama Batool1*, Saima Naz2*, Sheeza Bano1, Sadia Nazir3, Ghulam Abbas4, Ahmad Manan Mustafa Chatha5, Maria Lateef2 and Fatima Yasmin2

...rohita, employing probit analysis. Over a period of 90 days, mortality served as the primary toxicity criterion. The experiments were conducted under consistent conditions of pH (7), temperature (28.00.00°C), and water hardness (198.00 mgL-1), with three replicates for each dosage in the tests. Significant variations were noted in the LC50 values and lethal responses for both fish species. Catla catla exhibited mean LC50 and lethal concentrations of 55.78&...

Sandriana J Nendissa1,2, Meta Mahendradatta3, Zainal3, Februadi Bastian3

...t of fish meatball. Data analysis revealed that the addition of Tomi-tomi fruit tannin extract concentration was effective in enhancing the quality of tuna fish balls. The results of the analysis of variance indicated a significant impact (p >0.05) of Tomi-tomi fruit tannin extract treatments at various concentrations on the water content and protein of tuna fish meatballs However, there is no significant difference on th...

Feri Eko Hermanto1, Yuli Frita Nuningtyas1, Filoza Marwi1, Fajar Shodiq Permata2, Agus Susilo1, Muhammad Halim Natsir1*

...y promising targets. Our analysis pinpoints Interleukin-6 (IL-6) as the central protein governing gut health and mucosal immunity. Further exploration reveals that several bioactives, primarily from Teak Leaves and Ginger, form tight interactions with IL-6. This interaction presents an opportunity to influence the IL-6 signaling pathway, ultimately modulating mucosal immunity. Notably, ß-sesquiphellandrene, Luteolin 7-O-diglucuronide, Verbacoside, and Qu...

Ediset1*, Jaswandi2, Fuad Madarisa1, Amrizal Anas1, Rizqan2

...hod and a secondary data analysis approach. The population is 100 buffalo breeders who have adopted the AI. innovation. In comparison, the number of samples determined by the Proportional Sampling technique is 50 breeders from 5 sub-districts in Padang Pariaman Regency. Data analysis was carried out in a quantitative descriptive manner, namely the adoption rate of A.I. was measured by the percentage of adoption, and the succ...

Nadya Indriyani1, Sri Murtini2*, Sri Darwati3, Cece Sumantri3

... (HI) tests. Descriptive analysis was performed on production performance data and humoral immunity to estimate phenotypic correlation of the statistical method of Pearson correlation analysis. The results of IgY examination in men aged 27 weeks were 9.49 ± 1.37 mg/mL and IgY in women 9.36 ± 2.89 mg/mL. AbNDV ages 0 and 2 weeks at 1.34±0.95 and 1,370.95 LOG 2 GMT. At the age of 12 weeks, the body weight ...

Kashif Abdaal, Aneeza Batool, Muhammad Tariq Navid*, Saeed Ahmed, Asma Saleem Qazi, Waseem Safdar, Haidar Ali, Mobeen Ur Rashid, Somia Rafaqat 

...afety and post-marketing analysis (social and economic concerns). All these steps are important for a vaccine that leads to its success in preventing infectious diseases. It is crucial to address these challenges to make safer vaccines for effective infectious disease control and prevention to save humanity. Addressing challenges in vaccination coverage and gaps is crucial for global health. Vaccines have played a crucial role in global health preventing dise...

MUHAMMAD ASLAM1*, ZAHRA NOREEN2, AMINA ASGHAR1, ABRAR HUSSAIN2, RASHAD MEHMOOD3, MUHAMMAD USMAN KHAN4, KAYNAT SALEEM5, MUHAMMAD TAHIR HUSSAIN6* & ASMA CHAUDHARY7

...ontier molecular orbital analysis has been executed at B3LYP/6-311+G(d,p) level of theory. The global reactivity parameters were explored using the energy of frontier molecular orbitals. Natural bond orbital analysis has been carried out at B3LYP/6-311+G(d,p) level of theory to discover hyper conjugative interaction and stability of the title molecule. Moreover, the product showed the reverse urease inhibition activity.

...

LAILA SHAHZAD*1, ARIFA TAHIR2 & FAIZA SHARIF3

...84 annually. Descriptive analysis showed that 83.5% and 40.5% of respondents observed change in temperature and increase in annual rainfall respectively. Local residents of the area were more vulnerable to climate change and natural hazards due to their poor socio-economic conditions and previous disasters history. In order to adapt to change in climatic conditions, mitigatory measures should be taken to improve their living conditions. Deforestation should al...
ZAFAR IQBAL KHAN1*, KAFEEL AHMAD1, KHALID NAWAZ2, MUHAMMAD NADEEM3, ASMA ASHFAQ1,
BABAR MUNIR1, HAFSA MEMOONA3, MADIHA SANA3, FARZANA SHAHEEN1, NAUNAIN MEHMOOD4, HIRA MUQADAS4, MAHPARA SHEZADI5, IJAZ RASOOL NOORKA6, HUMAYUN BASHIR1,
MUDASRA MUNIR1, ILKER UGULU7 & YUNUS DOGAN7
... to the wheat plant. The analysis reveals that regular monitoring of sugar industry effluent is necessary to prevent the excessive buildup of metals in food chain which has broader implications in sustainable agricultural water management.

...

MUHAMMAD MANSHA1, MAIRA HAFEEZ1, QAMAR ALI1, ABRAR HUSSAIN1, MUHAMMAD TARIQ1 & ATIF YAQUB2

NAILA MALKANI*, IRFAN ISHAQUE, KHALID SHAHBAZ, RIZWAN ULLAH KHAN, ARIFA SHABBIR & ATIF YAQUB

...profile and histological analysis. The results showed that the values concerning liver function enzymes were significantly altered in wild-caught fish as compared to farm raised fish. Also, significant difference was noted in serum creatinine and urea values between the two groups. Histology of liver and kidney tissues revealed signs of chronic damage in the wild captured fish as characterized by lymphocyte infiltration and altered morphology of functional uni...

AAMIR MAHMOOD1, ZAHOOR AHMAD SAJID* & SHEZA AYAZ KHILJI2

... and pot experiment. The analysis spread out in a totally block design (randomized) with ten replicates for each salt (0 and 120 mM NaCl) and SA (0, 0.1, 0.5 and 1mM) treatment. The commercially available cultivar (cv. Faisal) of maize was planted in earthen pots for 15 days. After fifteen days of growth, seedlings were irrigated with saline water (120 mM NaCl) and SA was applied for 60 days simultaneously. To observe the germination, seed were also primed wit...

SHAGUFTA ANDLEEB1*, ASMATULLAH2, SHABANA SHAUKAT1 & SIDRA NAWAZ2

... Eosin. The histological analysis revealed that metformin could cause adverse malformations like pyknosis, necrosis, and steatosis.

...

Wesam Jasim Hansh 

...asurements and sequences analysis of 28S rRNA gene. Fasciola flukes were isolated from the livers of cows and buffaloes slaughtered in the municipality of AL-Nassiriyah abattoir during July to November 2022. Sixty Fasciola flukes including (30 from cows and 30 from buffaloes) were used in morphological study. Genomic DNA was extracted from 30 Fasciola flukes (15 from cows and 15 from buffaloes) and used to amplified the 28S rRNA gene and amplicons were sequenc...
Jaime Vera Chang1, Arianna Torres Coronel2, Luis Vásquez Cortez2,3, Kerly Alvarado Vásquez2,3 and Frank Intriago Flor4*
 
...ell and texture). In the analysis of variance (ANDEVA) it is concluded that in the bromatological analyzes T2 contains the greatest amount of energy with a value of 403.57kcal/100g, in T1 a value of 0.46% protein was determined and in T4 8.51% mg/ potassium. 100 g were obtained; These variables are important indicators for an energy drink. In the sensory evaluation of the drink, the non-parametric Kruskal-Wallis test was applied, showing that the best treatmen...

SYEDA NADIA AHMAD1, MEHWISH NASIR1, AQSA NAUREEN1, SHAGUFTA ANDLEEB4, KAUSAR RAEES3, TAHIR ABBAS2, ASMA YOUNIS1 & KHAWAJA RAEES AHMAD*1

...al sections. Statistical analysis has shown significant (p<0.001) variations in mean follicular size of thyroid indicating dose dependent depletion of colloid (thyroglobulin). Mean Cross-Sectional Area (MCSA) of follicular cells showed increase in DZ groups as compared to the control with maximum rise at 2.5 and 5mg exposure levels while the MCSA of their nuclei remained more-or-less constant. Statistical analysis has sho...

SYED SHAHID IMRAN BUKHARI1*, IQRA AZAM1, MUDDASIR HASSAN ABBASI1, MEERAB DAUD1, NADEEM SHEIKH2, ANDLEEB BATOOL3, RABIA MAHMOOD2 & MARYAM MUKHTAR2

...g gene β-actin. Gel analysis showed changes in the folds of IL-6 in hepatic mRNA compared to control. These finding led to the conclusion that Alloxan can induce an APR, which is evident by rise in expression of IL-6. Hence, extensive use of this food shiner should be avoided. Proposal of the current work was to determine the influence of Alloxan monohydrate on IL-6 gene expression, which is a major inflammatory marker.

...

MUBEEN RIAZ1, KIRAN ZAHID1, SUMAIRA ASLAM CHOHAN1, MUHAMMAD ASHFAQ*2, MUHAMMAD ALI2, RIFFAT SIDDIQUE1, NUDRAT ANEES1 & FARAH KHAN1

...ed products. During this analysis, two primers (OPH_01_F and OPH_01_RC) produced a total of 10 bands. Six of the bands were monomorphic i.e. 02 with the control plant, 02 with the 25mM salt stressed plant and 02 with the 50mM salt stressed plants. While with 75mM and 100mM salt stressed plants polymorphic bands were observed showing genetic variation. Profiles generated by the third primer i.e. OPI_05_R showed 4 monomorphic bands with control and NaCl stressed...

NAUSHEEN MAZHAR*, ALI IQTADAR MIRZA**, SAHAR ZIA*, ZAYANAH SOHAIL BUTT*, MEMUNA GHAFOOR SHAHID*** & ALI IMAM MIRZA**

*SHAJAHAN BAIG1, MUSHTAQ AHMAD SALEEM1, MUHAMMAD AZMAT ULLAH KHAN1 ZEESHAN NADEEM2, SUFIAN AHMED2, MEMUNA GHAFOOR SHAHID3 & TANZEEM AKBAR CHEEMA3

...ges, such as comparative analysis of (chemical) GM and non GM foods. These ways of identification of alterations commonly include DNA/RNA microarrays, metabolomics and protein profiling methods. These profiling methods are very useful but still studies on sensitivity, specificity and substantiation are needed. Furthermore, bioinformatics studies may be quite useful for the successful application of these profiling methods to analyze the safety of genetically m...

ASADULLAH KHAN1, MUHAMMAD AYAZ KHAN1, ALIA NAZ1, ABDULLAH KHAN1 & NAUREEN AURANGZEB1

...rrelation and regression analysis. Significant (P<0.05) variation in Pb and Cd concentrations in lichens along different roads were found. It was concluded that traffic density was responsible for Pb and Cd accumulation in lichen. Present findings further highlighted that there was significant variation in the chlorophyll content which amplify the heavy metal accumulation and subsequently inhibited growth of lichens.

...

*ARIFA ZEREEN1, ALMAS JAHAN1,SHEIKH SAEED AHMAD2 & ZAHEER-UD-DIN KHAN3

...wo Way indicator species analysis (TWINSPAN) and Canonical Correspondence Analysis (CCA) were used. Vegetation data obtained from forty quadrats was compiled. Thirty eight plant species belonging to twenty one families were recorded. TWINSPAN bifurcated the flora of entire study zone into two main communities that had been again separated into smaller i.e., sub communities. Canonical correspondence a...

FOZIA HUMAYUN1, ZARRIEN AYUB2, MUHAMMAD SAMEE HAIDER3 & SYED ABID ALI1*

...fication and biochemical analysis (Amino acid profiling). Macro/micro morphology of the shells was also studied by scanning electron microscopy and energy dispersive X-ray spectrometry. The analysis of nutritional status of the three Siphonaria sp. revealed quite similar profiles among the three Siphonaria sp with the presence of nine essential and nine non-essential amino acids. Our results successfully established the meth...

SIDRA MOQADDES*¹, RABIA ZULFIQAR1, MAWRA GOHAR1 & HINA QAISER1

...pectively. Socioeconomic analysis of data revealed that maximum number of patients visiting hospital for UTI belongs to villages (53%) followed by small cities (42%) and developed cities (5%). It is concluded that young females having low socioeconomic status and less awareness about hygienic measures are at great risk of UTI.

...

KOUSAR PERVEEN1*, MUHAMMAD AFZAL1, SANA SEHAR1 & SYED AMIR GILANI2

...elects nurses as unit of analysis because patients deal the most with nursing staff during their stay in the hospital. The findings of current study revealed that nursing staff have comprehensive understanding of a compassionate care behaviour. Nursing staff actively pursue to provide compassionate care to patients in distress which is motivated by some personal and professional factors. In addition, current study tries to explore the organizational factors th...

ZUBIA RASHID1, ZULFIQAR ALI MIRANI2, SITWAT ZEHRA1, SYED MUDDASSAR HUSSAIN GILANI3, ARIF ALI CHISHTI1, ABID AZHAR1 & SADDIA GALANI1*

.... Quantitative bacterial analysis of chicken gut revealed supplementation of PFA significantly increased (p<0.05) beneficial bacterial (Lactobacillus spp. and Enterococcus spp.) and reduced (p<0.05) pathogenic bacterial (E. coli and Campylobacter spp.) population versus AB and CON group. Overall, statistical profiling of gut bacteria depicted numerically increased beneficial bacteria in PFA group (79%) followed by AB (72%) and CON (65%) in chicken gut. I...

Uqba Mehmood

...f spore germination. The analysis of Spo mutants (strains which lack Sporulation (Spo) gene or have its inactive form) revealed that many of these strains did not produce extracellular proteases. Some evidence for the involvement of serine proteases in sporulation has been provided by inhibitor studies. Nevertheless variation in germination response of germination defective strains with different nutrients was recorded in ALA system, but overall responses of g...

ALIA NASEEM, *SUMERA IQBAL & KHAJISTA JABEEN

...d plants. In comparative analysis compost appeared as more beneficial for okra than farmyard manure. Overall, the application of compost at 5 percent was found to be effective in improving yield characters, chlorophyll and sugar contents of Cr affected okra plants.

...

1*AFIRA WAQAR, 2ADNAN RAUF, 3BUSHRA CHAUDHRY &4IKRAM-UL HAQ

...iate methods for genetic analysis.

...

Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1

...re analyzed with two way-analysis of variance. The result showed that equilibration time treatment and differential temperature of thawing yield good results on the motility and viability of Gaga chicken sperm on all treatments (P>0,01). Equilibration time of treatment for 2 hours was capable of preserving Plasma Membrane Integrity (PMI) and acrosomal integrity with a thawing temperature of 38 °C for 30 seconds or a 60 °C Thawing temperature for 5 s...

Karima Akool Al-Salihi1*, Luay Jumaah Jihad2, Abbas Najm Aldin Saleh3

... Iraq. A cross-sectional analysis was designed and the participants were invited to answer an online questionnaire on veterinary medical online education. Data of reported diseases were collected from veterinary hospital and ministry of health authorities. This study showed aggressive distribution of external parasites particularly ticks, and other diseases such as Theileria, Anaplasmosis, FMD, three-days sickness, contagious ecthyma, sheep pox, clostridium di...

Huda K. Khassaf1*, Zainab A.H. Al-Mousawi1, Sawsan A. Ali2, Anwar Nather Seiwan3

... measured. A statistical analysis of the thyroid gland tissue’s histological alterations was conducted. According to the findings, rats that got both SAL and ALA had higher levels of serum thyroxin T4, Free T3, and Free T4 than rats that did not get alA (p≤0.05). As well as decreased total bilirubin, Na+, and Ca+2 levels and increased K+ and Cl- levels (p≤0.05). Furthermore, ALA is an effective therapeutic medication that improves the histological ...

Tri Anggraeni Kusumastuti1, Ikuo Kobayashi2, Ahmad Juwari3, Lovin Dika Antari4*

...production centers. SWOT analysis is used to support a description of the condition of internal factors (strengths and weaknesses) and external factors (opportunities and threats) of animal husbandry in two countries. Data analysis was carried out descriptively and quantitatively. Primary and secondary data were collected from July to September 2023. The results showed that economic losses due to FMD in cattle in Miyazaki Pr...

Youfang Li1, Xu Li2, Shaik Althaf Hussain3, Jayasimha Rayalu Daddam4 and Zhigang Chen5*

...ne and serum biochemical analysis, tissue anti-oxidant, miR-21 as well as morphological examination. We found that administration of Sodium oxalate induced induced oxidative stress, alteration in the serum as well as urinary biochemical alterations as well as spiked expression of miR-21. The alterations were reversed in all the treatment group especially EGCG and EGCG-Quercetin. The reversal potentials of combinational therapy were found to be more effective t...

Firda Arlina1*, Mangku Mundana1, Linda Suhartati1, Agmelia Jasfaria2

... descriptive statistical analysis. The results showed that the qualitative variety of the Randah Batu Kokok Balenggek chicken reared by the Kokok Balenggek chicken Farmers in Solok Regency, West Sumatra, based on the Biriang color of 48.33%, the yellow/white color of the beak and shank of 96.67%, and Rantak Gumarang crowing type of 75%, the sound level ranges from 3-7 with an average of 4.63 ± 1.49. Meanwhile, the high variation in quantitative characte...

Ebban Bagus Kuntadi1, and Amam Amam2*

...alyzed using descriptive analysis and independent sample t-test using SPSS 26.0 software. The results revealed that the weight loss of the imported cattle on the MV Diamantina ship was 12.92 ± 5.66 kg per cattle, Barkly Pearl was 9.98 ± 5.05 kg per cattle, and MV Gudali Ex was 14.32 ± 4 .20 kg per cattle, while the weight loss of Brahman Cross heifers was 12.71 ± 6.19 kg per cattle, of steers was 12.15 ± 3.86 kg per cattle, a...

Wasan Ali Hasan1*, Manal Abdulkhaliq Ibrahim2 

...lung tissue histological analysis was conducted. The eosinophils, neutrophils, mononuclear cells, and total white blood cell count were all increased (P<0.05) in the positive control (B_group) when compared to the negative control group. Both D and E_groups, when treated with famotidine, showed a significant reduction (P<0.05) in the eosinophils, neutrophils, mononuclear cells, and total white blood cell count. Additionally, the histopathological examina...

Srisukmawati Zainudin1,3, Hartutik2, Edhy Sudjarwo2, Osfar Sjofjan2*

...ected and analyzed using analysis of variance (ANOVA). The results showed significant differences (p < 0.05) in nutrient digestibility when skipjack tuna offal meal was used as a fish meal replacement. In conclusion, fermented skipjack tuna offal meal was recommended as a source protein for local chickens.
 
Keywords | Fatty acid, Local chicken, Nutrient digestibility, Skipjack tuna, Omega
...

Md. Asadujjaman1, Md. Yeamin Hossain2*, Most. Farida Parvin3, Most. Shakila Sarmin2, Fairuz Nawer2, Wasim Sabbir4, Md. Ashekur Rahman2, Nur-E-Farjana Ilah2, Md. Joynal Abedin5, Md. Abdus Samad6 and Gitartha Kaushik7

Shahabaz Ul Haq1, Khurram Ashfaq1, Arsalan Khan2*, Adeel Khalid1, Shahrood Ahmed Siddiqui3,4, Raheela Taj5, Asad Ullah6, Hidayatullah Soomro7 and Muhammad Wasim Usmani8*

Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4  

...are (χ2) statistical analysis revealed that factors such as the age of chickens, seasons, floor condition of the chick’s house, flock size, bird health status, and practices for dead bird disposal showed no significant associations (p > 0.05) with the prevalence of the disease. In conclusion, this study helps to fill a knowledge gap in the prevalence and distribution of NDV in the Barishal district of Bangladesh, provides a framework for future lo...

Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4  

...are (χ2) statistical analysis revealed that factors such as the age of chickens, seasons, floor condition of the chick’s house, flock size, bird health status, and practices for dead bird disposal showed no significant associations (p > 0.05) with the prevalence of the disease. In conclusion, this study helps to fill a knowledge gap in the prevalence and distribution of NDV in the Barishal district of Bangladesh, provides a framework for future lo...

Muhammad Shuaib1*, Abdul Hafeez1, Woo Kyun Kim2, Aamir Khan3 and Abubakar Sufyan4

...s of the feces proximate analysis showed significantly higher crude protein during phases 1 and 2, while crude fiber during phase1 and 3 for the SH treatment groups. The crude fat had a significantly higher value for the control group than all SH treatment groups during all (three) phases. The ash amount was significantly higher in the 3 and 6% SH groups during phase 1 while 6% and 9% SH groups during phase 3 than in all other groups. The control group had (P&...

Muhammad Adnan1*, Muhammad Nauman Khan2,3, Barkat Ullah2, Faisal Zaman2, Alevcan Kaplan4, Hubert O. Dossou-Yovo5, Sajid Ali Khan Bangash6, Sana Wahab7, Mehreen Ghazal8 and Muhammad Hassan Sarfraz9

...e replicate. Through EDX analysis, it was noticed that the boron stress treatment exhibited the maximum number of elements detected in (T2, T5, and T6) wheat crops followed by (T0, T1, and T3). A total of 17 elements were detected and observed in wheat including Ca, S, K, Si, Na, Mg, Fe, Al, Cu, Cr, Cl, Mn, B, C, N, Co, and Ni in different treatments of B. It has been determined that the amount of each element varies according to the amount of B applied. Thus,...
Qun Lu1,4, Yang Zhou1, Hangyu Lin1,4, Zhong tang He1, Feifan Ma1, Yonghua Zheng1,2, Hongyu Tang1,2 and Tao He1,2,3*
... The linear discriminant analysis of otolith shape-index analysis can effectively distinguish the otolith shape of the two kinds of fish. The highest content of five chemical elements in otolith is Ca, and the other four elements are Mg, Sr, Ba, and Mn in turn. The results of one-way ANOVA show that, the molar ratio of each element to calcium (Mg/Ca, Ba/Ca, Sr/Ca and Mn/Ca) showed that there was significant difference betwee...

Zhenkun Zhao1,2, Ziniu Alimo2, Xinyue Zhao2, Haifen Qin1, Buddhi Dayananda3, Lichun Jiang1,2* and Wei Chen4*

...racters for phylogenetic analysis. Current research of avian phylogeny should focus on adding molecular markers and taxa samples and use both effectively to reconstruct a better resolution for disputed species.

...

Ali Hassan1, Javaria Ashraf1*, Salman Wahid1, Kamran Alyas1, Samaria Nisar1, Sadia Kanwal2, Nadia Hussain Ahmad2, Amna Bibi2 and Rameen Nawaz3

...ng ability and heterosis analysis are the important biological phenomenon that has been used to select the top performing parents and hybrids in different crop plants. In cotton, heterosis of morphological and yield related traits using line × tester design is very important to identify best performing parents in future cotton breeding program. Therefore, in this study five lines (MNH-1020, MNH-1035, FH-152, CIM-632 and BS-20) were crossed with three tes...

Shishir Sharma1*, Suk Bahadur Gurung2, Ritesh Kumar Yadav1, Bibek Phulara1 and Laxmi Prasad Joshi1

...zing principal component analysis and Ward’s clustering. Correlation assessment revealed a negative relationship between disease parameters and grain yield. According to the principal component analysis, three of the nine main components were significant (eigenvalues > 1) and accounted for 44.60%, 15.30%, and 13.60%, respectively of the total variance. PC1 comprised parameters including percent disease index (PDI), ...

Muhammad Usman1, Majid S. Hashmi1, Ayaz Ahmad1*, Fawad Ahmad2 and Zahid Alam1

...rage period. Statistical analysis confirmed that this particular formulation had a substantial impact on both the physicochemical and sensory characteristics of the drink. For commercialization of the product large scale evaluation should be done to validate these findings in addition to microbial analysis

...

Jin Jiao1, Jin Song1, Yuanyuan Cui1, Yilin Wang2, Yanling Li1, Jing Li1, Yan Su1, Xiaojing Du3, Yan Wang3, Guiping Sun3 and Minxiang Wei4*

... the multiple regression analysis showed that anxiety, depression and sleep disturbance jointly explained 44.8% of the variation in fatigue. To conclude sleep disturbance, depression and anxiety could be influencing factors of fatigue in patients with MI, which affect the rehabilitation process of MI, particularly in elderly patients. Medical staff should consider educating the impact of anxiety, depression, fatigue and sleep disorders on MI and how to determi...

Shahzad Aslam1*, Amjad Rashid Kayani2, Muhammad Irfan Ashraf3, Muhammad Azhar Jameel2 and Kiran Sahar4

...n combination with fecal analysis and visual observation for food composition. Both macroscopic and microscopic fecal analysis were carried out using 150 fecal pellets, collected from study area. The microhistological fecal analysis showed the presence of 29 dietary plant species as compared to 30 plant species observed in the field. No animal derived food component was noticed in the diet...

Philip O. Akporhuarho1, Ufuoma Godstime Sorhue1*, Adimabua Mike Moemeka2, Onyinye Stella Onwumere-Idolor3, Jonathan Ujomu1, Emmanuel Abadah1, Jennfer Aaron1

...rial for gene expression analysis. The feed intake data showed no significant changes (P>0.05);likewise the final weight, feed conversion ratio and feed efficiency, while weight gain was significantly different (P<0.05) with values of 1986.74 ± 13.54 (T2) to 2202.59 ± 53.34 (T1). Sensory attributes varied significantly (P<0.05) and improved with increased inclusion of Aframomum chrysanthum seed meal (ACSM). There were also no significant...

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

...cine strain, Correlation analysis, Clostridium chauvoei
...
Yanis Cruz-Quintana1*, Ana María Santana-Piñeros1, Byron Manuel Reyes-Mero1, Leonela Griselda Muñoz-Chumo1 and Lenin Cáceres-Farías1,2 
... host. Histopathological analysis showed the presence of slight hyperplasia and thickening of the lamellae of parasitized fish. The fish from El Coca farm showed significantly lower average sizes and weights than the fish from the other two farms, confirming the susceptibility of the smaller fish to these parasites. This is the first record of T. heterodentata for P. brachypomus in its entire range of distribution, and the first report of Tric...

Shah Zeb Khan1, Ali Hazrat1*, Farman Ali Khan2, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar3, Muhammad Ajmal Khan4 and Hayat Ullah1

...ansform (FT-IR) spectral analysis which gave distinct absorption of flavonoids functionalities. In antimicrobial studies, CR exhibited strongest antibacterial activity against E. coli, S. flexneri and S. aureus with inhibition zone of 25±0.75, 21±0.35 and 18±0.21 mm respectively. The same fraction showed very potent antifungal effect against A. niger and A. flavus with inhibition zones of 19±0.45 and 18±0.39 mm respectively. ...

Muhammad Ayyub, Mitha Khan*, Syed Abdul Malik, Sakhawat Ali, Syed Shamsullah, Mujeeb ur Rehman, Ikhlaq Ahmed, Ameer Uddin, Habibullah Kakar, Mohammad Zahid, Khalil Ahmed, Mana Khan, Mukhtiar Ahmed, Muhammad Rafiq Khetran, Zia ul Haq and Muhammad Azam

...epeats. The results from analysis of variance exhibits significant differences at (p<0.001 and 0.005) for all studied parameters. In hybrids, Benazir×Raskoo had the highest average value (92%) and in parents, TD-1 (89.66%) found best among hybrids. Similarly, correlation coefficient results indicated significant association among all genotypes for all characteristics. Most of the parameters among crosses showed highly positive significant heterosis. S...

Sarhad Journal of Agriculture

March

Sarhad Journal of Agriculture, Vol.40, Iss. 1, Pages 01-262

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