Joseph Andrew Bracken

Metaphysics for a World in Evolution
...rce and ongoing field of activity. The One can then be readily integrated into a hierarchical ordered system of fields within fields at all levels of existence and activity within nature. In the final part of the article, I indicate how this new field-oriented understanding of the relation between the One and the Many may help to solve controversial issues in natural science and Christian systematic theology.

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Muhammad Mushtaq

E-mail | mushtaq@uaar.edu.pk

... 25% reduction in burrow activity was achieved in case of groundnut – maize (1:1) grain bait loaded with 2% strychnine alkaloid and supplemented with 5% saccharin and a 10% reduction could be achieved without saccharin supplementation. In case of without pre-baiting trial, a 10% reduction in burrow activity was recorded in case of saccharin supplemented groundnut – maize grain bait impregnated with strychnine alk...

Asma Noshad1, Mudassar Iqbal1*, Zafar Iqbal1, Hamida Bibi2, Saifullah3, Salma Bibi4, Hamid Ullah Shah1

...valuate the insecticidal activity of the ethyl acetate extracts from fruit bodies, mycelia and fermentation filtrate obtained from Pleurotus ostreatus also known as the oyster mushroom. The percent mortality of Macrosiphum rosae (rose aphids) and the LC50 values were calculated and used as an indicative of insecticidal potential. The maximum mortality of aphids was achieved at 80 µg mL-1 and the LC50 for the fruit body ex...

Rabnawaz1*, Muhammad Jamal Khan2, Tahir Sarwar2, Mohammad Jamal Khan3

...ter than any other human activity. In Pakistan, the contribution of irrigated agriculture is more than 90%, thus the sustainable water supply to meet the food and fibre demand of the ever increasing population is a big challenge. Increase in crop water productivity (CWP) is one of the most important options for sustainable use of water resources in semi-arid environment. A field study was conducted during Rabi 2010-11 and Kharif 2011 crop s...

Youssef Lhor1*, Mounir Khayli1, Mohamed Bouslikhane2, Mehdi El Harrak3, Ouafaa Fassi Fihri2

Email: y.lhor@yahoo.fr

...dicator of the infecting activity of C. imicola. Collectively, the distribution patterns and emergence of BT can be predicted by the abundance of the Culicoides species in a specified locality so that control measures
can be implemented well in time to contain the disease spread.

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Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2

E-mail | Tomas.Jinnerot@sva.se

... which showed no cross-reactivity in silico or when tested with a panel of bacterial isolates. The limit of detection was determined to 4x103 bacteria per mL of nasal swab sample and less than 10 copies of target DNA per PCR reaction. Out of 23 isolates of B. bronchiseptica tested, one isolate from a hedgehog was not amplified. Sequencing of the 16S rDNA showed that the isolate was similar to strains of both B. ...

Mudasir Irfan Dar*, Fareed Ahmad Khan and Farha Rehman

E-mail | irfanmudasir@gmail.com

...The antioxidative enzyme activity of SOD, APX and GR showed increasing trend in a dose-dependent manner, attaining maximum at 40 g.a.i ha-1 of Imidacloprid treatment at day 7 after treatment (DAT) followed by 3 and 15 DAT, respectively. On the contrary, the CAT activity was increased at lower concentrations of Imida- cloprid but showed a dose dependence decline at higher concentrations of the insecticide at all the observed ...

Ali Mohammadi, Mohammad Hossein Ebrahimi

...mited degree of physical activity. However, most of young subjects are not recommended to be treated with this method owing to high level of physical activities. KineSpring knee implant system was designed for young subjects with knee OA to reduce knee pain, improve stability and knee function. The aim of this review article is to screen for the available information on knee implant system and to propose a suitable remedy for effective mana...

Murtaza Ali*, Yasser Durrani and Muhammad Ayub

...ensory parameters (water activity, ash content, percent acidity, pH, moisture content, reducing and non-reducing sugars, taste, texture, color and overall acceptability). Statistically minimum decrease was observed in water activity, pH, moisture content and organoleptic characters. While, stable increase was noted in percent acidity and ash content during storage. It was concluded and recommended on the basis of mentioned f...

Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1

...ed for its anticoccidial activity against Eimeria paillata induced infection in mice jejunum. Three different doses, 300, 600 and 900 mg/kg were used after infection of mice with sporulated oocysts. A dose of 300 mg/kg reduced the number of oocyst output in mice faeces by about 56.8 % and also improved the body weight. In addition, the root extract decreased the number of parasitic stages and the number of goblet cells in the jejunal villi in the infect...

Wan-Long Zhu* and Gao Wenrong 

...protein contents and COX activity both in liver and brown adipose tissue, suggesting that A. chevrieri was more sensitive to cold than that of photoperiod. Together, these data suggested that A. chevrieri mainly depend on increasing thermogenic capacity to cope with cold or winter condition.

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Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa

... They had no phosphatase activity, did not produce ‘film and spots’ and neither hydrolysed arginine nor urea and were preliminarily identified as members of the Mycoplasma mycoides sub-cluster which comprises Mmm and Mycoplasma mycoides subspecies capri (Mmc). Three (3) of the 8 Mycoplasma isolates were identified as Mmm using the IS1296F/R(all) and MM450/MM451 primer sets. The 574 bp product obtained by MM450/MM451 primer set gave two fragments (3...

Ai Ming Zhou1,2, Guang Wen Liang2, Ling Zeng2, Yong Yue Lu2 and Yi Juan Xu2*

...d the ghost ant foraging activity both on plants and the ground. Ant diversity in fire ant-infested plots was significantly reduced compared with in fire ant-free plots. Compared with in the no-ant plots, the colony growth rate of mealybug significantly increased, and the parasitism of mealybug was obviously decreased, both in fire ant-infested plots and in fire ant-free plots. Colony growth rate of mealybug in fire ant-infested plots was greater than fire ant...

Ayanda Opeyemi Isaac,1,* Oniye Sonnie Joshua2 and Auta Jehu2

 

... abnormalities like hyperactivity, abnormal swimming, restlessness, loss of equilibrum and haemorrhage. Observation of opercular ventilation count (OVC), tail fin movemant rate (TMR) and air gulping index (AGI) showed a marked difference between control and exposed fishes, indicating that the herbicides negatively impact on these parameters. These behavioural and morphological anomalies became more pronounced with increasing concentrations of the herbicides. M...

Ambreena Hafiz1, Tanzeela Riaz2 and Farah Rauf Shakoori1*

...nt of enhanced metabolic activity.

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Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2

... DPPH radical scavenging activity and in the MTT assay the extract has relatively low cytotoxicity where the IC50 value of extract against Vero cells was 1078.69 µg/ml. Furthermore, the extract exhibited a considerable anti-HSV-1 and anti-HSV-2 activities at the concentration of 25 μg/ml. In conclusion, our findings indicate that the methanolic extract of Capsicum annuum could be used in combination with standard drug to treat herpes infection.

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John G. Bruno

... was significant cross-reactivity between the various captured serotypes, each of the anti-LPS aptamer candidates showed a preference for its cognate E. coli serotype. Results suggest that these aptamers may be useful for enriching specific E. coli pathogens during aptamer-magnetic separation in food samples, leading to a greater ability to detect these serotypes if coupled with genetic identification techniques such as PCR or gene probe hybridization.

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Dein Simon

...undamentalism Project: Reactivity, dualism, absolutism and inerrancy, apocalypticism. Additionally both underscore the role of evidentialism. From my analysis of these characteristics I conclude that the movement is fundamentalist

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Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

...s still unknown. Reduced activity of antioxidants enzymes because of polymorphisms in antioxidant genes and variations in levels of metals and minerals creates oxidative stress may play some role in progression and pathogenesis of RA. The objective of this study was to investigate the role of Superoxide dismutase 1 (SOD1) gene polymorphism [rs2070424 (–251A/G)] in RA and to compare the levels of metals and minerals among its various genotypes in RA patie...

Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1

...ies level. Antimicrobial activity of aqueous and methanolic plant extracts of Camellia sinensis (Green tea), Syzygium aromaticum (Clove) and Mentha piperita (Peppermint) was evaluated against identified isolates. Agar well diffusion assay was used to monitor the antimicrobial activity of these strains both in mono culture and mixed culture. Streptomycin sulphate (10 μgml-1) and Amphotericin B (5 mgml-1) were used as posit...

Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

Rashid Mahmood1*, Saima Asad2, Ghulam Sarwar1, Waqar Ahmad1, Ziyad Abdul Qadir1, Ammara Balouch1 and Muhammad Khalid Rafique1
...er to study the foraging activity of honeybees in the field condition, the number of pollen foragers returning to the hive entrance was recorded for 10 min duration on hourly basis between 0800 h to 1600 h for 3 days per week. The data was collected at 0800–1000 hours (early morning foraging activity), 1000-1200 hours (late morning foraging activity), 1200-1400 hours (Noon foraging <...

Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki

...howed moderate to high reactivity, suggestive of domestic pigs’ exposure to tick-bite. Antibody reactivity’s were found in eight of the 10 states studied and all the states have favourable climatic conditions for soft ticks’ survival and national parks harbouring wild swine (warthogs) able to maintain the developmental cycle of these ticks. This provides some evidence of interaction between the host and the...
Fermín Lopez-Uriostegui1, Jesús T. Ponce-Palafox2,*, Fabiola Lango-Reynoso1, María del Refugio Castañeda-Chavez1, Virgilio Eugenio Arenas-Fuentes3 and Fernando Vega-Villasante4
...es feed 24 h, but forage activity differs in intensity in the two species. In M. tenellum repletion index was greatest during the day and M. americanum in the night.
...
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
Memoona Akber1, Muhammad Mushtaq1,*, Muhammad Sajid Nadeem1, Amjad Rashid Kayani1 and Noor-un-Nisa2 
...ter season. Reproductive activity revealed that the males were recorded reproductively active throughout the year while more than 50% specimens of females were recorded inactive throughout the year; yet lactating and pregnant females, and scars in the oviduct were recorded throughout the year indicating that Norway rat reproduces the year around in the urban environment of Rawalpindi, Pakistan.
...

Muhammad Waqas1*, Anwar Ali Shad1, Omaira Bashir2 and Mohsin Iqbal3

...t (87.52 %). Antioxidant activity of carotenoids from tomato peel was assessed by measuring Rancimat’s test and diphenyl-1-picrylhydrazyl (DPPH) by using 50 to 200 mg/kg carotenoids in sunflower oil and spaghetti. The sunflower oil containing 50 to 200 mg/kg carotenoids showed higher antioxidant activity in comparison to 200 mg/kg Butylated hydroxytoluene (BHT). Optimum cooking time, its weight and swelling index were ...
Xiang Nong1,2,3,*, Yao-jun Yang1,2, Guang-you Yang3, Feng-zheng Chen2, Mei Tang1,2 and Gang Wang2
...i>. The insecticidal activity of stigmasterol was weaker than that of ageraphorone compound obtained from E. adenophorum Spreng. Through preliminary separation. However, in the experiments, we found an interesting phenomenon, that is, the dead state of mites treated with stigmasterol was similar to that of mites of the positive control group treated with fenvalerate. Furthermore, the dead state of mites treated with stigmasterol was different from t...
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...cluded the antibacterial activity of apricot (Prunus armeniaca L.) and its by-products. The aim of the study were to test the activity of chlorogenic acid isolated from apricot cv. Habi, against different food borne pathogens and quantification of the chlorogenic acid (CA) using High Performance Liquid Chromatography (HPLC). The chlorogenic acid was isolated using resin. The recovered amount of compound was calculated...

A. K. Chaubey and Satyandra Kumar

Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...Petri plates. Antifungal activity was tested by dual culture of wilt fungus with antagonist fungus and bacteria isolates in PDA media plates. In in-vivo test soil was amended with antagonist and wilt fungal and bacterial isolates 2 x 106 CFU/ml and 2 x 108 CFU/ml respectively as talc formulation @ 0.2% (w/w). Twenty one days old tomato seedlings were transplanted after dip treatment in antagonist fungal and bacterial CF in earthen pots filled with amended soil...

S. Subharani, S. S. Thorat, N. Abem, L. Amit Kumar and T. K. Singh 

A database on parasitoid of insect pests of crops in Manipur, India
... distribution, period of activity, parasitoid behavior and its biology etc. Image of the parasitoid species are also provided for helping in easy identification of the species. Keywords: Database, insect-pest, parasitoids, Manipur
 

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Madhuban Gopal, Rajesh Kumar and Arunava Goswami 

Nano-pesticides - A recent approach for pest control
...ealed many fold improved activity of nanopesticides against fungi and mites as compared to commercial sulphur.

 

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Goutam Mondal

Plant growth promoting activity of some indigenous Tricho-derma isolates and their field performance against sheath blight of rice in old alluvial zone of North Bengal
...gal and their biocontrol activity were tested in dual culture and found effective against soil-borne plant pathogens, namely, Fusarium oxysporum f. sp. ciceri (Padw.) Matuo. and Rhizoctonia solani Kühn. Efficacy in plant growth promoting activity of all the isolates was also tested on cauliflower (Brassica oleracea var botrytis L.), chilli (Capsicum frutescens L.) and tomato (Lycopersicon esculentum Mill.) with culture ...

A. Sajeena and T. Marimuthu

Efficacy, stability and persistence of Ganosol, a Ganoderma based fungicide against plant pathogens
...tability. The antifungal activity of the formulation persisted up to seven days after spraying the formulation on rice plants. The formulation retained its 100% antifungal efficacy up to three months of storage. Surprisingly, spraying cowpea plants with the formulation as simultaneous spray reduced the number of lesions produced by Groundnut Bud Necrosis virus in cowpea by 88.39% when compared to the inoculated control, suggesting the antiviral potential of th...
Zhensheng Liu1,2, Hui Gao1,2, Jiapeng Dong1, Mingming Zhang3 and Liwei Teng1,2,*
... We studied the seasonal activity of blue sheep from May 2010 through August 2012 to test the activity budget hypothesis, which predicts that females spend more time on feeding and that the activity synchronization of same-sex groups is higher than for mixed-sex groups. Our results support the hypothesis. Both males and females spent most of their time on feeding and moving. Feeding behavi...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...luation of antibacterial activity of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin. Camel raw milk produced 2.4 mg/mL of lactoferrin. The lactoferrin isolated from camel milk exhibited promising antibacterial activity against E. coli and Staph...

Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3

...f the broiler birds. The activity of the superoxide dismutase, catalase and glutathione peroxidase in the broiler blood was increased as the citrus waste level in the feed of the broilers was increased. The citrus waste addition in the feed of the broiler have non-significant effects on the growth parameters, however it has positive effects on the antioxidant enzymes profile of the broilers.

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Aneela Qureshi1, Ammara Ainee1*, Muhammad Nadeem1, Masooma Munir1,2*, Tahir Mahmood Qureshi1, Saqib Jabbar2
...ying fiber content water activity, texture, color, moisture, fat, protein, ash, NFE and fiber content changed significantly (p<0.05). Gross energy level in cake which is desirable by consumers was reduced but high fiber cake was dark colored, low in volume and with increased hardness. Treatment (T3) containing 5.6g grapefruit albedo powder shows significantly (p<0.05) higher sensory scores in term of color (8.02±0.50), flavor (8.04&plus...
Shuangye Wang1, Yunlin Zhao1, Zhenggang Xu1,2,*, Li Li3, Liang Wu1, Choucang Duan1 and Jiao Peng1

 

...d after 13:00 hours when activity behaviour increased. The adaptation of Milu was documented to a certain extent, it was found that the animal’s behavioural rhythms changed during acclimation to the new habitat. It is likely that resting and feeding behaviours were influenced by temperature.
...
Saba Irshad1,*, Ayesha Ashfaq1, Ammara Muazzam1 and Abida Yasmeen2
...diarrheal and anticancer activity of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.), used in Pakistani cuisine to enrich flavour and to treat infectious wounds. Their crude ethanol and methanol extracts were tested against five important bacterial and fungal strains. Both extracts showed maximum antibacterial activity against B. subtilis, E. coli and S. aureus. All alcoholic...

Wali Khan1, Naushad Khan2 and Shaista Naz2*

...ngaged in the beekeeping activity. It also provided multiple sources of employment mainly in honey transportation and marketing, thus beekeeping not only served as an income generating activity but also an employment generating activity in present and in future. The major constraints in beekeeping were the expensive inputs, pest attack, lack of modern production and rearing techniques, and...
Muhammad Abdullah1,*, Rashid A. khan2, Muhammad Rafay3, Tanveer Hussain3, Tahira Ruby4,Fariha Rehman5, Sangam Khalil3 and Sohail Akhtar6
...osystem. To analyze this activity present work was planned to collect the preliminary information about habitat requirements and different breeding aspects of Cattle egret (Bubulcus ibis). The fieldwork was performed for two years (2012, 2013) at three different stations in the surrounding area of Faisalabad. Based on results it was concluded that Cattle egrets are colonial breeders and colonies were monospecific with no other ardeidae members nesting i...

Jawad Anwar* and Zafar Iqbal 

...tigate the antibacterial activity of Trichoderma harzianum against phyto and human pathogenic bacterial strains. T. harzianum was grown under different conditions and their extracts were evaluated, using disc diffusion method against tomato wilt causing bacteria (Xanthomonas campestris and Clavibacter michiganensis). Extracts that resulted in strong activity were fractionated into acetonitrile and ethyl acetate followed by t...
Furqan Sabir1, Muhammad Tayyab1,*, Bushra Muneer2, Abu Saeed Hashmi1, Ali Raza Awan1, Naeem Rashid3, Muhammad Wasim1 and Sehrish Firyal1
...>TN showed maximal activity at 80°C in 50 mM sodium acetate buffer pH 6. Presence of Fe3+ or Cu2+ showed an enhancing effect on the PHYTN activity. Thermostability studies demonstrated that PHYTN retains 88% residual activity when the protein was incubated at 80°C for 1.5 h in the presence of 1.5 mM Fe3+. The enzyme exhib...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*

 

...rug that can inhibit the activity of cytochrome P450 aromatase, so as to affect the production of estrogen. In the present study, to detect the effects of letrozole on gonad differentiation, 5dph fry of carp were exposed to different concentrations of LET. At 130 dph, sex ratio was recorded. The results showed that continuous exposure to LET induced the changes of sex ratio. High concentration of LET can promote male-biased sex ratio. In order to reveal the mo...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...itro anti- bacterial activity of Manuka honey against NDM-1 producing clinical isolates of Pakistan. A total of five carbapenem resistant bacterial species; E. coli, K. pneumoniae, E. cloacae, A. baumannii and P. aeruginosa, were isolated and identified using VITEK 2 compact system. Carbapenamase and metallo-β-lactamase (MBL) production by these isolates was confirmed by modified Hodge’s test and disc potentiation method, respectiv...
Rana Muhammad Aadil1,2*, Xin-An Zeng1, Saqib Jabbar3, Akmal Nazir2, Abid Aslam Mann2, Muhammad Kashif Iqbal Khan2, Abdullah2, Amna Ramzan2
...total anthocyanins, DPPH activity, total antioxidant capacity (TAC), total flavonoids (TF) and total flavonols as equated to thermal and control, while ºBrix, pH and acidity remain unchanged in all HPP treatments with control and thermal. After this, we suggested that HPP treatment (250 MPa/60 °C/3 min) has a potential against thermal to progress the value of grapefruit juice and could be applicable at industrial scales.
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Zahid Mehmood*, Alam Zeb and Muhammad Ayub 

...c compounds, antioxidant activity, ascorbic acid) and organoleptic characteristics (flavor, color, texture and overall acceptability). The results revealed decrease (P<0.05) in pH, ascorbic acid, total phenolic content, antioxidant activity while increase in acidity, total soluble solids during six months storage. Decrease in score of organoleptic attributes was recorded during the storage. The results revealed that (P<...
Ayesha Aihetasham1,*, Muhammad Saeed Akhtar1, Maryam Umer1, Khalid Zamir Rasib2 and Muhammad Imran Din3
Mahreen Yahya, Noor Abid Saeed*, Sajid Nadeem, Muhammad Hamed and Sajid Shokat 
...d reduced photosynthetic activity. Photosynthetic rate and chlorophyll contents were significantly higher in insecticide treated plots. Maximum cost-benefit ratio was found (1:9.2) in NW-1-8183-8 when treated against aphids. Wheat varieties, Galaxy-13 and Faisalabad-08 were found tolerant against aphid damage. Wheat variety Lasani-08 should be avoided because it was found susceptible to aphid infestations, and give low net yield. It is inferred that infestatio...

 Zeenat Bano1,*, Sajid Abdullah1, Waqas Ahmad2, Muhammad Anjum Zia3 and Wardah Hassan1

...so exhibited highest SOD activity in all the organs as compared to farm and hatchery fish. The activity of CAT enzyme was higher in kidneys and gills of farm fish compared to the liver of hatchery fish. The maximum POD activity was recorded in liver, kidney and gills of hatchery fish. This study reveals that fish could acquire higher uptake of metals due to excessive pollution of heavy met...
Carolina A. Bonin1,2, Eric A. Lewallen2,3, Andre J. van Wijnen3, Marta Jussara Cremer4 and Paulo C. Simões-Lopes5*
...ing as the most frequent activity in the estuary, totaling nearly two thirds of all records. We also identified two sites of Guiana dolphin habitat preference in our study area, where sightings remarkably totalled > 62% of observation records. These sites (especially Guaraqueçaba Bay) were not only important for feeding, but also for S. guianensis socialization. The detection of these key areas should facilitate both local and broad-scale effo...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...rvive and maintain their activity for several hours in tissue samples. For this study piglets without colostrum feeding were selected. Primary cell culture and subculture was performed from the intestinal epithelial cells of piglets. Then the effects of intestinal trefoil factor (TFF3) in intestinal disease was explored. Porcine intestinal epithelial cells were treated with lipopolysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 m...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2 
...eks for growth promoting activity. Increased mammary growth was observed in test female non lactating mice, as compared to controls when placental lactogen was applied. Increase weight gain for visceral tissues like kidney, liver, muscle and lungs was also observed, as compared to control subjects. It was also recorded that the exogenous supply of bubaline PL reduces fat cells in the hypodermal layer of skin.
...
Toshina Mushtaq, Muhammad Javed and Sidra Abbas*
... 30 days. The peroxidase activity was found to be significantly increased both in liver and kidney after ZnCl2 treatments. Statistically significant increase in the liver and kidney peroxidase activity of 96-h LC50 exposed fish were found as 0.621±0.004UmL-1 and 0.219±0.002UmL-1, respectively as compared to the control fish liver (0.123±0.002UmL-1) a...
Aqsa Imtiaz and Abdul Rehman*
...howed maximal keratinase activity at 37°C and at pH 8. An increase in keratinase activity of B. subtilis BML5 was determined in the presence of Ca2+, Mg2+, and Zn2+ while keratinase activity was inhibited by added copper ions. A 130-kDa protein was detected from feather-treated bacterial cells, suggesting its role in degrading feather. ...
Saleh S. Alhewairini
...ot affected with minimal activity in both applications compared with control. Moreover, no mortality of adults emerging from pupae was recorded and they only showed detectable reduction on their movement in both field treatments. Finally, this study would recommend the use of oxamyl in managing and controlling the infestation of RPW.
...
Tariq Mahmood*1, Syed Wasif Ahmed Shah1, Muhammad Rais1, Hira Fatima1, Faraz Akrim1 and Muhammad Sajid Nadeem2
...e monitored for breeding activity of the particular bird species through clutch size, incubation period, and hatching success. Sixty-two active nests of various bird species were recorded; common myna Acredotheris tristis, house crow Corvus splendens, Indian robin Saxicoloides fulicatus and black drongo Dicrurus macrocerus preferred Kikar Acacia nilotica and Eucalyptus camaldulensis for nesting purpose while black kite...
Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1
... FPase (2.526 IU/ml/min) activity was found at pretreatment conditions of 0.6% NaOH conc., 10% biomass loading and 4h reaction time while CMCase (2.803 IU/ml/min) at 1% NaOH conc., 15% biomass loading and 6h of reaction time. The proposed model was found highly significant as revealed by their F-values, P-values and R2 values. Results suggest the efficiency of Bacillus subtilis K-18 for cellulase production from eucalyptus leaves and it would be fruitful for f...

Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3
 

...ment. The maximum CMCase activity was 1.757 1U/ml/min under conditions of 0.6% acid concentration, 10% substrate concentration, and resident time of 4h. The maximum FPase activity was 2.015 1U/ml/min under conditions of 0.8% acid concentration, 10% substrate concentration, and time of 6h. This showed that peanut shells could successfully be used as substrate for cellulase production in submerged fermentation.

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 Asma Akhtar, Abdul Rehman

...te dehydrogenase (G6PDH) activity was increased to 279% (1f) and 150% (2b) when ompared to the respective control indicating the ability to tolerate tellurite tress. Differential pattern of bacterial proteins in tellurite stress was obtained by SDS-PAGE. Both bacterial strains can be utilized to convert toxic tellurite into its elemental form from the contaminated sites.

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Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
...ide on peroxidase enzyme activity in the fish, Labeo rohita. For this purpose, four groups of Labeo rohita (one-year old) were exposed to different treatments as 96-h LC50, 2/3rd, 1/4th and 1/5th of LC50 concentrations of iron chloride for 30 days in the glass aquaria with three replications for each treatment. After 30-day exposure of iron chloride, the fish organs viz. kidney and liv...
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
...p<0.001) hypoglycemic activity with no considerable effect on mean body weight of rats (ns, p>0.05) as compared to diabetic rats. The AGE treatment has significantly reduced the blood glucose level (56%), however, the EGE treatment was found effective in normalizing the lipid contents of diabetic rats. The black cumin oil treatment has resulted in significant (p<0.001) increase (48%) in body weight of diabetic rats, but found less active in regulating...

Farah Rauf Shakoori, Momina Shokat, Faiza Saleem and Tanzeela Riaz

...enzyme possessed maximum activity. Two bacterial isolates XPB-GS02 and XPB-CW01 with maximum activity ratio showed 18.3 and 12.2 U/ml xylanase activity, respectively. Maximum xylanase was produced in medium M1 containing xylan after 48 hours of incubation. Maximum amount of enzyme was produced by XPB-GS02 at 45ᵒC, pH 9 using 1% xylan concentration, while XPB-CW01 produced maximum amo...
Tasleem Akhtar*, Muhammad Asif Aziz, Muhammad Naeem, Muhammad Sheraz Ahmedand Imran Bodlah
...orsata (0.98%). Peak activity of the insect visitors was observed at the mid of the day i.e., 12:00 pm. The activity of managed A. mellifera started to increase from the third week of blooming (20th Jan, 2015) and reached to maximum in the sixth week (10thFeb, 2015). Among the abiotic environmental factors, temperature had a strong, significant and positive correlation with the foragin...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...her it has antibacterial activity. The lethal dose and toxicity determination of the color extracted from marigold showed that it is safe for consumption as no clinical symptoms were observed in rabbits after giving the maximum dose of 1000ml of color.
...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
..., EB 11, EB 13 with hyperactivity as compared to wild bacterial strain. Out of the total 15 isolates of EtBr-treated strains, EB4 gave the maximum enzyme activity (7.3 ± 0.005 U ml-1) with a zone diameter of 6 mm. While UV6 was found to be the best UV-induced mutant with an enzyme activity of 20.333 ± 0.006A U ml-1 and 16 mm of zone diameter. ...

 Muhammad Asim, Muhammad Asif, Muhammad Munir* and Muhammad Aslam**

...ODI-3 retained more radioactivity as compared to Williams-82 in inoculated as well as uninoculated plants. The stem and roots of Williams-82 plants had a percent distribution of ABA that was higher than NODI-3. The phloem sap of Williams-82 had higher accumulation of 3H-ABA at all stages after treatment. The basal levels of ABA in phloem sap were lower in NODI- 3 than in Williams-82. Inoculation further accentuated this difference. The major changes in ABA lev...

 Rahila Nazli, Tehmina Sohail, Bushra Nawab and Zahra Yaqeen*

...tigate its antimicrobial activity against gram +ve and gram-ve bacteria. The study was also extended to some species of fungi. Results showed that the activity was more pronounced against gram +ve organisms and fungi. Maximum inhibition activity was calculated against all the groups of organisms, which was between 0.5 and 1mg ml-1 against bacteria and 2.5 mg ml-1 against fungi. It is there...
Promy Virk
...aluate the anti-diabetic activity of the prepared nanopcomposite. Antidiabetic potential of orally administered bulk aqueous extract of Trigonella (TBE) and Au/Ag nanocomposite with Trigonella seed extract (TN) on streptozotocin (70 mg/kg) induced diabetic rats was assessed for a period of 7 weeks. Metformin was used as a standard drug. The results of Zetasizer and Transmission and Scanning Electron Microscopy images showed the presence of spheri...
Abul Hassan Faiz*, Fakhar-i-Abbas and Lariab Zahra Faiz
...effects of rat burrowing activity on, plant biomass, saturation, pH, electrical conductivity, organic matter, potassium and phosphorous concentration. These significant differences in soil composition have long-term effects on ecosystem sustainability and productivity of vegetation.
...
Amtul Jamil Sami1,*, Sehrish Bilal1, Madeeha Khalid1, Muhammad Tahir Nazir1 and A.R. Shakoori2

 

...ct of saponins. The AChE activity of T. castaneum was inhibited by the saponins and follows competitive inhibition kinetics. In case of A. mellifera enzyme activity was not inhibited. In vitro and in vivo inhibition was observed for T. castaneum at larval stages, in dose dependent and time dependent manner. LC50 was determined to be 0.7ppm. To investigate the different effects of sap...
Nazish Shaukat1, Muhammad Javed1,Faiza Ambreen2* and Fariha Latif1
...ct of lead on peroxidase activity (antioxidant enzyme) in the gills and liver of Labeo rohita. Experiment was conducted in the laboratory by exposing four groups of Labeo rohita to different doses of PbCl2 viz. 96-h, 2/3rd, 1/4th and 1/5th of LC50, separately, for 30 days and compared with control group. After 30 days, fish was sacrificed and their gills and liver isolated to determine...

Akhlaq Ahmad, M. Anwar Arain*, Rahila Nazli**, Syed Anser Rizvi***,
Mubarik Ahmed* and Farzana Ibrahim****

Corresponding author: dr.akhlaqahmad@yahoo.com

COST EFFECTIVENESS OF UNDER-SHEET AND WHOLE GODOWN FUMIGATION OF BAGGED WHEAT
...showed no sign of insect activity during the 8 months storage. From the study, it is concluded that under-sheet fumigation of stacks of bagged wheat is a very cost effective and feasible method for protection of stored cereals, and need to be implemented at the provincial and national levels.

 

...

 Ambreen Akhtar Saddozai, Saeeda Raza* and Shahzad Arshad Saleem**

MICROBIAL COUNT AND SHELF LIFE OF STRAWBERRY JUICE
...uence of microbiological activity on different quality factors of strawberry juice during storage. Strawberry was purchased from local market and its juice was kept for two weeks at room temperature to check its shelf life. Total plate count (TPC), pH, flavor, color, yeast and moulds were checked weekly. Quality changes of strawberry juice during storage can be caused both by microbiological and physiological processes. At day 0; pH 3.2, color bright 2 -1 red,...

 Abid Hussain*, Khalid Mahmood Aujla* and Sonila Hassan**

ECONOMICS OF COW MILK PRODUCTION IN SINDH AND AZAD JAMMU & KASHMIR
...on is profitable farming activity in irrigated areas of Sindh and mountainous-AJK with benefit-cost ratios of 1.5 and 1.4, respectively. While, benefit-cost ratios of cow milk production were 1.0 and 0.7 in desert and coastal areas of Sindh, respectively, indicating subsistence nature of cow farming in these ecologies.

...

 Muhammad Sharif*, Muhammad Azam Niazi* and Abdul Jabbar**

SEAFOOD EXPORTS: CHALLENGES AND WAY FORWARD
... most important economic activity along the coastline of provinces of Sindh and Balochistan. This paper assesses current stock of seafood production, seafood exports, direction and future prospects of seafood exports from Pakistan. The production of inland and marine fish was 729,000 t in 2012-13. Not only seafood output in the country is low but dwindling to the disastrous level. Export of seafood has increased from 131,620 t (a value of US$ 315.5 million) in...

 Mazher Abbas*, Irfan Mehmood*, Arshed Bashir*, Muhammad Ather Mehmood* and Sonila Hassan* 

WOMEN COTTON PICKERS PERCEPTIONS ABOUT HEALTH HAZARDS DUE TO PESTICIDE USE IN IRRIGATED PUNJAB
...marily a female specific activity in all cropping zones of Pakistan. Women cotton pickers mostly belong to poor rural society involved in this labour force to feed their families. Cotton pickers in Pakistan face some serious health related problems due to heavy use of pesticides on cotton crop. The present study was designed to investigate the problem faced by women cotton pickers and their role in household decision making. Overall 150 women cotton pickers we...

 Muhammad Zahid Kiani*, Arshad Ali**, Tariq Sultan** and Muhammad Munir Ahmed***

CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM ROOT SYSTEM OF SUNFLOWER (HELIANTHUS ANNUS L) GROWN UNDER SALT AFFECTED AREA OF PAKISTAN
...owed maximum bio-control activity (fungal growth inhibition) than other isolated strains.

...

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...AK strain larvae, the TE activity was inhibited with depletion of total protein contents and elevation of FAA contents. In FSS-II larvae, the effect of abamectin on levels of α-amylase, glucoamylase, AkP, total protein and soluble protein remained non-significant. The activities of TE and AcP were reduced at both doses, while activities/levels of CE reduced at LC and FAA increased 10 at LC . It is concluded that abamectin affected the overall body 20 fun...

Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***

ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
...his study, the antiviral activity of Viro Care GZ-08™ was checked up invitro, in-ovo and in-vivo. The cytotoxicity assay of the product was performed using Vero cell line. All the trials revealed that the stock solution and 1:2 dilution of GZ-08™ had some antiviral activity as well as were cytotoxic. As the concentration decreased, cytotoxicity as well as antiviral activities were lost. Based on these findings, i...

 Sameera Arshad*, Iftikhar Hussain*, Maqsood Anwar* and Sarwat Naz Mirza*

HABITAT SURVEY FOR RECOGNIZING BIRD ATTRACTANTS AROUND BENAZIR BHUTTO INTERNATIONAL AIRPORT, ISLAMABAD, PAKISTAN
...ighly conducive for bird activity which in return is a serious safety concern for aircraft operation. Outcome of this study could be incorporated into off airfield bird management programme for monitoring bird activity and land use practices around the airfield.

...

 Ambreen Akhtar Saddozai*, Amer Mumtaz*, Saeeda Raza* and Shahzada Arshad Saleem** 

MICROBIAL COUNT AND SHELF LIFE OF PHALSA (GREWIA ASIATICA) JUICE
...ill two weeks. Microbial activity in phalsa juice increased while organoleptic attributes such as texture (mouth feel), flavour, taste, colour and overall acceptability of phalsa juice were decreased during the study period. The pH and TSS value were decreased from 3.99 to 3.54 and from 11.22 to 9.55, respectively after 2 weeks -1 -1 storage. Total plate count also showed decline from 6.2×10 to 3.2 x 10 -1 cfuml whereas yeast and mould counts increased s...

Sumia Bint Zaman*, Waqas Farooq*, Sidra Majeed*, Hasnain Shah*, and Abdul Majid**

ASSESSMENT OF AGRICULTURE SERVICE PROVIDERS' TRAINING ON WATER CONSERVATION TECHNOLOGIES IN POTHWAR REGION
... included as part of the activity. The information for paper is collected from opinions of the participants before and after the training. Descriptive analysis alongwith paired 't' test was used to measure the effectiveness of training. Results indicated higher satisfactory level as majority of the participants were strongly agreed (73%) and agreed (23%) that trainings were participatory and skill development oriented and as 96% agreed or strongly agreed that ...

 Tasawar Sultana*, Farah Deeba* and S.M. Saqlan Naqvi*

HIGH-THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION OF MEDICAGO TRUNCATULA IN COMPARISON TO TWO EXPRESSION VECTORS
...peroxide dismutase (SOD) activity in transformed progeny. 

...
Anum Rafique, Sajid Abdullah, Khalid Abbas, Huma Naz* and Wardah Hassan
...treated fish had highest activity as 508.33 and 427 UmL-1 while highest specific activity was 1105.06 and 1055.55 Umg-1 with 1.98 and 2.70 fold purification, respectively. Recovery was 70% and 68% in kidney of control and Pb+Cr treated fish, respectively. After characterization it was observed that SOD had wide range of pH i.e. 4.0-8.5 and maximum activity of 5...
Rabia Faiz and Dilara A. Bukhari*
...rmining the insecticidal activity of cyt positive B.t. strains against 3rd instar larvae of mosquito. Larval mortality was noted after 24 h and GCU B.t. 4 (400± 1.15) was found to be the most toxic against 3rd instar larvae of mosquitoes. Spores LC50 values of other isolates were 451± 0.90 (GCU B.t. 1), 511± 0.85 (GCU B.t. 2), 525± .013 (GCU B.t. 5), 582&plus...
 Nazia Qamar1, Sher Khan Panhwar1,* and Ralf Riedel2,*
... showed a higher feeding activity. The IRI showed fish, followed by crustaceans, to be the most important food item for S. commersonnianus and S. tol. This study is to offer baseline data toward implementing a fishery in Pakistan for current and future generations.
...
Zhiping Hu, Hussain Ahmad, Jingfei Zhang, Lili Zhang and Tian Wang*
...thione peroxidase enzyme activity was significantly increased in T1 group compared to CON group at 42d. Malondialdehyde content of liver in T1 group was lower than that of CON group. These results suggested that dietary supplementation of 10 mg/kg natamycin had a positive influence in broilers.
...
Chuanpeng Zhou1, Heizhao Lin2,*, Zhong Huang2, Jun Wang1, Yun Wang1 and Wei Yu2
... hepatic pyruvate kinase activity peaked 6 h after oral administration in both doses of glucose groups and thereafter started to decrease 24 h after oral administration. While the hepatic PEPCK and G6Pase activities decreased 9 h after oral administration in both doses of glucose groups and then returned to the level at 0 h (P<0.05). These results suggested that glucose oral administration resulted in prolonged hyperglycemia and high level of hepatic...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
...enzyme exhibited optimum activity at 55 °C between pH 8-9. The effect of metal ions on the activity of AMYSBS showed that Co2+ remarkably enhanced the enzyme activity and 500µM was recorded as optimal Co2+ concentration for the maximal activity of AMYSBS. Presence of ionic (SDS) and nonionic (Tween-20...
Shahzad Mahmood1, Memuna Ghafoor Shahid1, Muhammad Irfan2, Muhammad Nadeem3*, Quratulain Syed3
...C and attained 96% activity at 40oC for 70min. Metal profile showed that α-amylase activity was enhanced in the presence of Ca2+, Cs1+, Mn2+ and Co2+, whereas inhibited in the presence of Na1+, Mg2+, Ag1+ and Cu2+. Enzyme kinetics revealed that crude enzyme exhibit Km and Vmax of ...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...nation of its biological activity. SUMO-IFNα2-Tα1 was successfully expressed in soluble form with IPTG induction to final concentration 0.5 mM at 37oC for 4 h and purified using affinity chromatography. SUMO tag was removed from SUMO-IFNα2-Tα1 by SUMO protease and recombinant IFNα2-Tα1 was collected in flow through by affinity chromatography. The MW (~23 kDa) of IFNα2-Tα1 was determined by 12 % SDS-PAG...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...nsible for antimicrobial activity and biofilm dispersion. Overall the results indicated that natural extracts from these Streptomyces strains and their active components can be exploited for the preparation of anti-caries products like mouth washes and tooth pastes etc.
...
Hongqun Li1, Xiaoli Liu2,*, Zhenmin Lian3,RenheWang4, Yongbin Wang4, Yongyao Fu1 andDingyi Wang5
...st that moderate logging activity and prohibition of local peoples’ firewood collection in the core areas may provide some optimal nest habitat for the brown-eared pheasant.
...
Huma Naz1,*, Sajid Abdullah2, Sidra Naz3, Sidra Abbas2, Wardah Hassan2, Shakeela Perveen2, Moazama Batool1, Laiba Shafique2
...ral changes and catalase activity in liver, kidney, gills, brain and muscle of the fish were also studied. The mean 96 h LC50 and lethal value for C. mrigala was determined as 47.56±0.04 and 93.89±0.07 mgL-1, respectively. The selected concentration of Fe+Ni+Pb+Zn concentrations showed strong and direct relationship with, fin movement followed by mucous secretion, hyperactivity and ...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...nificantly reduced while activity of trehalase were significantly increased after treatment with sub lethal dose of λ-cyhalothrin as compared to control. The metabolic derangements induced by sub-lethal dose of λ-cyhalothrin suggest that infestation caused by T. granarium in godowns could be overcome by calculating lethal dose of λ-cyhalothrin.
...

Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1* 

...d better anticlostridial activity in terms of reduced CP counts in intestine and alleviation of intestinal damage caused by infection. In conclusion, the EOs are effective feed additives to treat and/or prevent NE in poultry. Further studies on the effect of EOs on other enteric infection, intestinal microbiota, poultry feed components, and poultry meat quality are required. 

...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,* 
...kaline phosphatase (ALP) activity and increase in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in the serum along with decrease in level of antioxidant enzymes and significantly increase in the MDA contents in TD afflicted chickens compared to control group. The SM administration to TD affected birds significantly ameliorated lameness, stimulated ALP level with a decrease in ALT and AST contents, increase in antioxidant parame...
Abdur Rahman1,*, Ibrahim Sadi Cetingul2, Ismail Bayram2, Cangir Uyarlar2, Abdil Burhaneddin Akkaya2, Eyup Eren Gultepe2, Hikmet Keles3, Aykut Ulucan4 and Zafar Hayat1
...s may have the potential activity to modify the gut morphology and can result in better performance of the quail by increasing the nutrient absorption from the intestine.
...
Souheila Slimani1,*, Sonia Hamouda1, Chahrazed Souadi1, Sara Silini2, Cherif Abdennour2 and Leila Delimi3
...ng photoperiodthe sexual activity of the control lasted only 04 weeks, characterized by significant increase in the testicular volume, followed by spontaneous gonadal regressionup to week 10. Consequently, an azospermia and lack of germ cells in control birds was noticed,which confirm the testicular regression.In the treated groups, thiram delayed the refractory phase along the experimental period, but testicular weight were superior in the treated pigeons com...
Nina Bao1,*, Yongguang Yin2 and Panpan Wang3
...rebroside. Meanwhile, bioactivity showed that the extraction has antibacterial activity, and the purified one is better than the crude one.
...
Changqing Zhao*, Zhuochi Chen and Yubin Li
...jerky; the acid protease activity was slightly higher than the neutral protease activity in the fermented jerky; four and fifteen volatile compounds were detected for the non-fermented and fermented jerky, respectively. The important compounds affecting the flavor of fermented jerky included DL-Glutamic Acid, Phenylethyl Alcohol, 1-Octen-3-ol, Nonanal, Pentanal, 3-Methyl-Butanal, and 2-Pentyl-Furan. The study results demonst...
Yang Yang1,2, Yun Liu1, Mingxu Zhou1 and Guoqiang Zhu1,*
...> caused decreased QS-II activity.
...
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
...op pests, however, their activity is deteriorated rapidly when exposed to the sunlight. Many UV protectants were used to improve the efficacy of these viruses under field conditions. The objective of the current study was to evaluate the effectiveness of plant extracts to improve the persistence of Spodoptera littoralis multiple nucleopolyhedrovirus (SpliMNPV) against beet army worm, Spodoptera exigua(Hübner) under harsh sunny field c...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...7 showing maximum lipase activity of 71±3.55 U/g, was selected. Resistance in the hyper producing strain was developed by exposing it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optimum conditions at 30°C temperature, pH 7, and incubation period 64 h, the lipase activity was increased up to 124±2.48 U/g. The crude enzyme was purified by ammonium sulfate preci...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...to enhance antimicrobial activity against these pathogens.
...
Adem Aksoy1, Yakup Erdal Ertürk2, Selim Erdo─čan3, Ecevit Eyduran3 andMohammad Masood Tariq4,*
...erformed only beekeeping activity. The four most influential predictors in the MARS were age of enterprise (100%), number of full beehives (100%), other races (97%), and other works except for beekeeping (90%). The best performance order was MARS (r=0.920) > CART (r=0.619) > multiple linear regression (r=0.286), which indicated that MARS outperformed other approaches. MARS reflected that the main and interaction effects of socioeconomic (age of enterpris...

Muhammad Zahid1, Naeem Iqbal1, Sohaib Muhammad2*, Summiya Faisal3, Wajid Mahboob3, Makhdoom Hussain4 and Zaheer ud din Khan2 

...rates. Nitrate reductase activity and total soluble proteins were increased with increase in sugar treatments under drought. Osmotic and water potentials were reduced under drought but foliar glucose sprays of 10 mM and 50 mM applied at reproductive phase significantly reversed the adverse effects of drought. Gas exchange characteristics including CO2 concentration, transpiration and photosynthesis rates were raised by glucose treatments under irrigated and no...
Kiran Aqil1, Muti-ur-Rehman Khan2, Asim Aslam2, Aqeel Javeed5, Rizwan Qayyum1, Farooq Yousaf,1 Farkhanda Yasmeen1, Muhammad Luqman Sohail3 and Sajid Umar4,*
...current study, antiviral activity of six dilutions of Nigella sativa (N. sativa)alcoholic extracts against the PPRV was investigated in vitro. Vero cell lines infected with PPRV were treated with six dilutions alcoholic extracts of N. sativa (200, 100, 50, 25, 12.5 and 6.25 µg/mL). The antiviral effects were judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric (MTT) assay. Plaque reduction assay was ...

Qaisar Jamal1,Salman Ahmad1, Nazma Habib Khan1, Sobia Wahid1, Muhammad Ikram2, Sadia Rehman2, Syed Basit Rasheed1 and Akram Shah1,* 

...gate in-vitro anticancer activity of two transition metal compounds of derived Schiff bases; NM-3 (Tetrakis (2-{(E)-[(2- {[(Z)-(2-hydroxynapthyl) methylidene]amino} phenyl)imino] methyl}phenol) Copper(II) and NM-4 (Tetrakis (2-{(E)-[(2- {[(Z)-(2-hydroxynapthyl) methylidene] amino}phenyl)imino]methyl} phenol) zinc(II)), against human THP-1 leukemia cell line in-relation to miltefosine (standard chemotherapy). Mean % inhibition for NM-3 against THP-1 was higher ...
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
...ge with no fall of phage activity.
...
Faxiang Wang, Yan Chen, Sai Chen, Xianghong Li, Jian Yu, Jianhui Wang and Yongle Liu*
...e Ca2+-ATPase activity of protein decreased gradually during the storage period. The high MW proteins easily degraded during the cold storage, and the proteins with low MW were relatively stable but still gradually degraded. So the key time point for cold storage of grass carp is approximately 4-6 days.
...

Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...ixture on catalase (CAT) activity in gills of carnivorous fish Channa striata was observed. Fish were exposed to sub-lethal concentration (1/3rd of LC50) of DM+END mixture for 14-day. CAT was partially purified by using ammonium sulphate precipitation technique and kinetic characterization was also performed against different pH and temperature. CAT activity was measured spectrophotometrically at A240 nm. Results showed that...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...shown to possess laccase activity. In this study, we have cloned and over-expressed the CueO of locally isolated Klebsiella pneumoniae KW strain. The CueO protein was purified to homogeneity by nickel affinity chromatography. Enzyme assays of CueO protein with phenolic substrates revealed its laccase activity. The kinetic studies showed Km value of 0.2µM, kKcat 0.68 S-1 and Kcat/km 1.2S-1
Nimra Naseer, Adeela Fatima and Imran Sajid*
...ignificant antimicrobial activity against MDR bacterial pathogens. Secondary metabolite profiling of each methanolic extract by TLC and HPLC-UV signified the presence of unique and diverse metabolic compounds. The study predicts that the seactinomycetes strains are a potential resource to cope up with evolving multi drug resistant pathogens. Furthermore, these secondary metabolites can be identified by comparative studies and these actinomycetes can be grown o...

Zulqarnain and Zafar Iqbal* 

... their potent antifungal activity against the Fusarium wilt of tomato. Six distinct fungal species including Penicillium sp., Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Alternaria solani and Pythium aphanidermatum showed growth inhibition (%) against the pathogen. Penicillium sp. showed maximum growth inhibition (63.85 ± 3.26 %) and its extract showed 9.20 ± 0.14 mm zone of inhibition at 1000 mg·L-1 against the Fusarium ...
Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...peroxide dismutase (SOD) activity, glutathione (GSH) and adenosine triphosphate (ATP) concentration, as well as nitric oxide synthase (NOS) activity and nitric oxide (NO) concentration catalyzed by NOS. Based on biochemical analysis of the above, results showed that O2- production could be induced significantly after cold stress at 15°C and heat stress above 30°C. SOD ac...
Mehwish Aftab, Tariq Mahmud*, Muhammad Asim Raza Basra, Aqsa Gulzar, Sumaira Basharat and H.M. Junaid
... base showed antioxidant activity against diphenylpicrylhydrazyl (DPPH). The results showed that metal complexes are good antioxidant agents as compared to Trolox (standard) and ligand was less active as compared to Trolox. The Schiff base ligand and its metal (III) complexes showed antibacterial activity against E. coli, S. aureus by disc diffusion method and antifungal activity ag...
Ashraf M. Mounir, A.N. El shahat* and A.M. Abdul Azeem
...ammatory and antioxidant activity. Gamma-irradiation is used for decontamination of Boswellia tears to achieve satisfactory microbiological quality and public health safety. Hence, examining the effect of gamma-irradiation on the contents of Boswellia oleo-gum resin and also investigating the role of gamma-irradiate frankincense aqueous extract (GFAE) against ISO-induced MI in rats were the two aims of this study. The total phenolic content and t...
Farah Mansha, Abdul Rehman*
...es showed highest enzyme activity at pH 7. Isolates H4 and M4 showed maximum enzyme activity at 55°C while H1 showed highest activity at 70°C. Chitinase activities exhibited by H1 and M4 are stimulated by the presence of Zn2+ ions, while the presence of Ni2+,Cd2+ and Cu2+ did not show any positive effect. However in H4, presence of Zn&sup...

Asma Sohail1*, Aina Rafique2, Kashif Sarfraz Abbasi1 and Maryum Arif3 

... potential antimicrobial activity. Effect of drying methods (sun-, oven- and freeze drying) on antioxidant and antimicrobial activities of date fruit powders from three varieties Zahidi, Aseel and Muzafati were investigated. The DPPH activity was significantly higher in Muzafati sundried date powder, whereas maximum phenolic and flavonoid contents were significantly higher in Muzafati freeze dried date powders. Results sugge...

Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1 

... reduce their biological activity on heating, volatilization, and other parameters like oxidation and UV rays. This limits the commercial application of these oils. To overcome such problems encapsulation could be a gentle approach. Prior to encapsulation, oil extraction was done by using five different solvents including ethyl acetate, n-hexane, methanol, diethyl ether and chloroform in which ethyl acetate yielded 53% oil. Pumpkin seed oil was encapsulated us...
Muhammad Khan
...its potential anticancer activity. This review will mainly focus on the anticancer activity, cellular targets and anticancer mechanism of PSD-A which may help the further design and conduct of research and repositioning it for oncological clinic.
...

Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3  

...d in vitro antibacterial activity against two phytopathogenic bacterial species (Clavibacter sp. and Xanthomonas sp.) and a human pathogenic bacterium (Escherichia coli) in 2016. Experiment was carried out by using complete randomized design with three replications in vitro. Phytochemical analysis reveals that the methanolic fraction of oil possesses metabolites like carbohydrates, amino acids, alkaloids, flavonoids and glycosides. To test the antibacterial
Tünay Konta┼č A┼čkar1,*, ┼×inasi A┼čkar1, Olga Büyükleblebici2 and Murat Güzel3
...or myeloperoxidase (MPO) activity in CVL. Therefore investigation of this enzyme activity in dogs with leishmaniasis may be used for the diagnosis and inflammatory changes in dogs with VL.
...

Hareem Mohsin, Azka Asif*, Warda Fatima, Sarooj Nadeem, Anjum Nasim Sabri

Assessment of biocalcification by the water absorption test using microorganism from oil contaminated site
...nditions produced by the activity of the enzyme urease allowed the reaction between calcium and carbonate ions that leads to the precipitation of the calcium carbonate. The water absorption test was performed to check the presence of a protective layer that provides resistance to water penetration and corrosion to cement. The samples treated with calcium and urea for one hour were found better as shown by less absorption of water compared with the untreated s...

 Muhammad Mohsin Ahsan1, Hafiz Muhammad Tahir2, Muhammad Khalid Mukhtar1

Effect of lunar cycle on active population density of scorpions, their potential prey and predators
...f the lunar cycle on the activity of O. odonturus. Young (Immature)
individuals of all scorpion species were always high in numbers at night, even under moonlight conditions. The
activity of scorpion prey had been always high during dark nights. No difference in numbers of scorpion predators
(shrews, lizards, centipedes, mice and rats) was noted in the presence or absence of moonlight, but they were

 Mirfa Murtaza1, Sajid Abdullah1, Wardah Hassan1*, Khalid Abbas1, Huma Naz1, Muhammad Anjum Zia2

Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
...ign: justify;"> The activity of digestive enzymes was determined in Hypophthalmichthys molitrix, Catla catla and Cyprinus carpio fed
with different feed ingredients. There were two glass aquaria (3×2×2 ft) for each species and ten fish in each
aquarium. Fish in one aquarium were fed on rice polish and fish in second aquarium were fed on corn gluten @ 3 %
of their wet biomass for 70 days. Results showed that fish wet weight...

Muddasir Hassan Abbasi1, 2, Noor Fatima1, Syed Shahid Imran Bukhari1, Asma Rashid khan3, Nadeem Sheikh2*

Variations in proteins and transaminases following experimental induction of Bisphenol A in mice
...n and aminotransaminases activity. The sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was also run to analyze protein bands. Statistically significant
increase in total protein contents, albumin and aminotransaminases were noted in sample in comparison with control.
Comparative analysis between experimental and control samples revealed protein fractions (KDa) of 149, 132, 101,
72, 75 and (163, 114, 91, 75, 72,...

Dilara Abbas Bukhari1, Abdul Rehman2*

Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus
...hecked for antimicrobial activity against bacterial strain, Bacillus subtilus. Biochemical techniques, thin layer chromatography, high performance liquid chromatography and gas chromatography/mass spectrometry, were employed to study fungal exudate. A total of fifteen different metabolites were detected in the sample by GC/MS. The detected metabolites could be classified into chemical groups of 2 esters, 4 alcohols, 3 benzoic acids, ketone, aldehyde, phenyl an...

 Haniya Mazhar1, Naaz Abbas2, Zahid Hussain3, Amir Sohail4, Syed Shahid Ali4*

Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
...tion. The maximum enzyme activity (34.93 U/ml) was shown by B. subtilis using SSF with soya bean meal. The lipase production by other low cost agro-industrial waste, including sunflower meal, wheat bran, oat bran, rice bran and sugar cane bagasse was 23.83, 12.17, 10.40 10.00 and 16.23 U/ml, respectively. The lipase production by B. subtilis using peels of different fruits, including banana, orange, water melon and melon as carbon source, was 27.17, 21.37, 10....

 Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1

Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
... and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume of normal saline solution. Rats of 12h and 24h group were killed after 12 and 24h of TAA administration
respectively. Bl...

 Mohsin Arshad1, Muhammad Irfan*2, Asad-Ur-Rehman1, Muhammad Nadeem3, Zile Huma3, Hafiz Abdullah Shakir4, Quratulain Syed3

Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification
...ve maximum
CMCase activity (8.0 ± 0.32 IU) after 48h of fermentation period at 37oC with
agitation speed of 140 rpm by using medium components III (M-III) and also
medium M-IV. The highest enzyme production was observed in the following order;
sugarcane bagasse > wheat straw > rice husk, wheat bran > soybean meal >
corncobs. The crude CMCase enzyme was applied for saccharification of
agricultural was...
Haniya Mazhar1, Naaz Abbas2, Tehseen Zamir3, Zahid Hussain1, Syed Shahid Ali4*
...ons and lipolytic enzyme activity shown by the bacterial isolate (PCSIR NL-37) from the soil of Lahore (Punjab, Pakistan) are being reported. Lipolytic bacterial strains (100) isolated from soil and water samples from oil industry, mobile oil filling areas and kitchen wastages were screened on tributyrin agar and rhodamine agar plates. The strain PCSIR NL-37 was identified as Bacillus cereus following morphological, biochemical and molecular characteriz...
Muhammad S. Waqas, Ali A.Z. Shoaib, Xinlai Cheng, Qianqian Zhang, Asem S.S. Elabasy and Zu-hua Shi*
... during the time of male activity, may be an effective practice for the management of this pest.
...
A.M. Abdul Azeem, A.N. El Shahat* and Ashraf M. Mounir
...c content andantioxidant activity of pomegranate peel powder as well as to evaluate the effect of gamma-irradiated pomegranate peels extract (GPE) against biochemical alterations of lead induced testicular and hepato-toxicity in rats. From the results, it was obtained that gamma irradiation induced significant increase in the total phenolic content and antioxidant activity of pomegranate peels powder. Concurrent treatments o...

Akshay Sharma*, Mohit Mahajan, Madhumeet Singh and Pravesh Kumar 

...scularity and functional activity of corpus luteum present on the left ovary. Ultrasonography was employed at weekly interval to judge the viability of embryo (up to 42 days), fetus (after 42 days) and late embryonic mortality, if any. Sexing of the twins was done on 56th and 70th day post artificial insemination and two male twins were confirmed on the basis of genital tubercle, median raphe, scrotal and penis impression. With the aid of ultrasonography and c...
Fatih Korkmaz1, Veysel Parlak2, Özgür Kaynar3, Arzu Ucar2, Gonca Alak1,* and Muhammed Atamanalp2
...ease in bacterial growth activity, physicochemical values (TVB-N, TBARS and pH) and prolonged shelf life in a natural preservative dependence were observed in the fillets. Generally all the test parameters, such as microbiological, physicochemical as well as the lowest mean values were identified in the coatings of the fillets with turmeric and cardamom (p<0.05). Control and quinoa groups resulted in increased number of bacteria and chemical values compared...
Jair Millán-Orozco1,*, Jersson Millán-Orozco2 and Luz María Tejada-Ugarte3
...i>P<0.05) grazing activity was observed during September (38.9±11.4 min/h), while in May (27.1±14.3 min/h) was the month with least activity (P<0.05). Within days, the highest (P<0.05) grazing activity was observed at 06:00, 12:00 and 18:00 h (34.9±12.0, 31.8±12.9 and 34.5±10.6 min/h, respectively), while 09:00 and 15:00 h (28.6...
Aqeel Mehboob, Syed Muhammad Zaka*, Muhammad Sarmadand Maryam Bajwa
...as oviposition deterrent activity against the S. litura was found when the above mentioned botanicals were tested.
...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
...xplore the antibacterial activity against the four cariogenic bacteria in terms of acid producing and biofilm. The antibacterial activity of capsaicin was evaluated by the minimal bactericidal concentration, change of pH in the first 12 h and OD values of biofilms. Besides, fluorescent images of exopolysaccharides and living bacteria of Streptococcus mutans were measured with confocal laser scanning microscopy (CLSM)....
Zhi YiJin1, Shao MingQin1,*, Cui Peng2 and Chen Bin1
...w time was budgeted, and activity rhythms were established in wintering Mandarin Ducks (Aix galericulata). This was done using instantaneous scan sampling in the Poyang Lake watershed in the Jiangxi province, China. The results showed that the Mandarin Ducks’ predominant behaviors were resting (36.56%), swimming (21.75%), foraging (20.88%), and maintenance (15.58%), indicating that during the winter, Mandarin Ducks spend most of their time accumul...

 Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2

...method. The anthelmintic activity was analyzed by adult motility assay (AMA). Levamisole was used as positive control to compare the efficacy of CEE of both plants. Adult worms were treated with different concentrations i.e., 2, 4, 6 and 8 mg/ml of ethanolic extract and motility of the worms was examined after every two to eight hours. Both A. lebbeck and C. sinensis exhibited 88% and 95% mortality at 6 & 8 mg/ml after 8 hrs of treatme...

Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1

...dants. Highest enzymatic activity was observed in treated plants followed by inoculation with MYMV. Increase in resistance by the application of SA and BTH, antioxidant enzymatic activities of SOD, POD, CAT were also increased for second week. This revealed that SA and BTH can be used as a potential source for the activation of resistance for better management of MYMV by improving protection level by the induction of systemic acquired resistance. 

...
Fariha Latif* and Muhammad Javed
...red in terms of catalase activity in the gills, liver, kidney, muscles and brain. Among the fish species, C. mrigala accumulated significantly higher concentration of all metals as compared to L. rohita and C. catla. Bioaccumulation of all metals among the organs followed the order: liver>gills>kidney>brain>muscles, however, metals followed the trend for their accumulation: Cd>Cr>Cu>Pb. Antioxidant (catalase)
Muhammad Usman Ahmadand Ikram-ul-Haq*
...st oxyrase producer with activity of 0.41±0.008U/mL/min with 41% reduction in dissolved oxygen at pH 7.5, temperature 37°C, 25mM lactate as H+ donor after 20 min. This strain was later on confirmed by 16S ribotyping procedure. Oxyrase was used for improved cultivation of anaerobic bacteria by employing Hungate Technique where its antioxidant potential was compared with common reducing agent Cysteine-HCl (Cys-HCl). Four anaerobic bacterial...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
...mg/ml. The antimicrobial activity was assessed by disc diffusion method. Cephalosporin production was estimated by using spectrophotometric technique.
...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...rated the optimal enzyme activity at 90°C and pH 4.8. The presence of cobalt enhanced the cellulase activity and 2.5 mM cobalt was recorded the optimal concentration for the maximal cellulase activity. SDS-PAGE analysis confirmed the molecular weight of recombinant protein as 39 kDa. The protein was found thermostable which retained more than 70% residual
Iqra Bano1,*, Moolchand Malhi2, Pershotam Khatri3, Saeed Ahmed Soomro2, Hira Sajjad4, Ambreen Leghari5, Muhammad Awais2, Safia Kandhro6 and Shakeel Ahmed Lakho5 and Munaza Soomro7
...athione peroxidase (GPx) activity as well the testicular GPx activity was significantly increased in SY as compared to C. Furthermore, the serum T-SOD activity was significantly elevated in SY as compared to C. Moreover, the T-SOD activity in testicular tissues was also significantly increased in SY as compared to C. The results revealed that the SY supp...
Tahira Moeen Khan* and Amat ul Mateen
...ignificant antibacterial activity against four bacterial strains three gram negative (Escherichia coli, Proteus mirabilis, Salmonella) and one gram positive (Clostridium tetani). The minimum concentration of leaf extracts needed against these bacterial strains were also calculated. The present study indicated that Melia azedarach and Morus nigra leaf extracts can be used successfully for nanoparticles synthesis and CuO as significan...

 Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2 and Muhammad Anjum Zia3

...r purification, specific activity of enzyme was 1750.13 and 1272.72 U mg-1 in kidney of metal exposed and control fish, respectively. The enzyme from metal exposed kidney was 2.68 fold purified with 58.73 % age recovery. Results of characterization showed that SOD had broad range of pH from 4 to 8.5 but show maximum activity at pH 7 and 7.5 for stressed and control fish, respectively. At 30°C
Jie Yang
...r bacteria. Bactericidal activity of human PGLYRP-1, PGLYRP-3, PGLYRP-4, and PGLYRP-3:4 for both Gram-positive and Gram-negative bacteria require Zn2+. In addition, for killing of Gram-negative bacteria, Zn2+ cannot be replaced by other cations, but for killing of Gram-positive bacteria, Zn2+ can be partially replaced by Ca2+, which have been proved. Then, the effect of human PGLYRP1 on several human diseases (inflam...
Muhammad Shahid Nadeem*, Maryam A. Al-Ghamdi and Jalaluddin Azam Khan
...However, the glutaminase activity of enzyme can cause serious side-effects triggering a search for highly specific and more stable enzyme. In the present study the gene consisting of 972bp coding for 323 amino acids was PCR amplified from Geobacillus thermodenitrificans DSM-465 was PCR amplified and recombinant plasmid pET22b-Asn was prepared. L-asparaginase was produced in BL21 (DE3) RIL codon plus strain of E. coli as heterologous protein under...
Muhammad Nasir1,*, Ata-ul-Mohsan2, Munir Ahmad2, Shafqat Saeed3, Muhammad Asif Aziz2, Muhammad Imran4 and Umer Ayyaz Aslam Sheikh4
...estris during flight activity period and solitary phase of development. Results showed that survival rate and colony initiation rate of the queen was highest at 28 ºC and 30 ºC. Different parameters in the initial stage of colony growth like pre-oviposition period and emergence timing of the first worker were observed best at 28 and 30 ºC. Number of workers produced in the first brood and in the total lifespan of the colony was also observed...
Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2, Wardah Hassan3, Moazama Batool4, Shakeela Perveen5, Sadia Maalik4 and Sajida Mushtaq4
...fos) investigated on the activity of antioxidant enzymes viz. superoxide dismutase, peroxidase, catalase and glutathione S-transferase in different organs (liver, gills, kidney, brain, heart and muscle) of fish, Labeo rohita. The LC50 of chlorpyrifos+endosulfan mixture was calculated as 1.95±0.02 μgL-1 for 96 h with the 95% confidence limits. The fish expose to the mixture (1:1) for 96-h. The results obtained from this st...
Fan Yi1,2, Xiaobin Yang1, Shigen Ye1, Hua Li1 and Ruijun Li1,*
...es count, serum lysozyme activity, phagocytic rate, phagocytic index, respiratory burst activity of head kidney cells, compared with the basal feed group (P<0.05 or P<0.01). The challenge test results also showed that the 0.5% and 1% HCT feeding had higher immune protection against Edwarsiella tarda infection; and the relative protection ratios were 60% and 50%, respectively. It was also obtained t...
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
...ein. Higher level enzyme activity was recorded at 25°C, pH 7.0 when the cells were induced with 0.5 mM IPTG with 22h post induction incubation. Supplementation of LB medium with 1% glucose and yeast extract enhanced the production of recombinant thermostable cellulase. Enzyme showed strong potential for its use in paper and poultry feed industry. Under the optimal conditions we could able to produce 48 U/mL of recombinant cellulase.
...

Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1 

...d for their antagonistic activity against Fusarium oxysporum f.sp. alium cepa. They all were found to inhibit fungus showing inhibition zone 4.09 -74.97%. Hence, on the basis of these studies, it can be concluded that these bacteria can be used as a biocontrol agent to manage other fungal diseases of agricultural crops also. 

...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...rified polygalacturonase activity was found to be 4.5 and stable within pH range 3.5-5.5. Temperature dependent studies revealed temperature optimum of enzyme to be 40°C and stable up to 60°C. Among substrates, polygalacturonic acid was established as the best substrate for polygalacturonase showing its specificity in the hydrolysis of polysaccharide galacturonate chain. The presence of Na+1 and K+1 enhanced polygalacturonase
R─▒fat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...o PCR results, caspase-9 activity increased incrementally in groups given 50 μM of TA. The increase in the apoptosis of LNCaP cells may be due to the rising amount of TA concentration. In our study, in 25, 50, and 100 μM TA groups, cyclooxygenase-2 (COX-2) decreased incrementally compared to the control group. Considering overstimulation of COX-2 paths in emergence of cancer or in metastasis, this study shows TA has an antiproliferative and stimulating e...
Ailing Huang, Lingyu Meng, Wei Zhang, Junyan Liu, Guangyao Li, Huihua Tan, Wen Lu and Xialin Zheng*
...n other four pesticides; activity of glutathione-s transferase (GST) was inhibited by abamectin, while the trend was in the opposite direction for other four pesticides; activity of superoxide dismutase (SOD) was promoted by beta-cypermethrin and chlorpyrifos, and inhibited by abamectin and bisultap; Activity of catalase (CAT) was promoted by beta-cypermethrin, chlorpyrifos and bisultap, i...
Yuyu Wang, Gangchun Xu*, Zhijuan Nie, Quanjie Li, Nailin Shao and Pao Xu*
...eters, digestive enzymes activity and antioxidant status of largemouth bass (Micropterus salmoides) reared in an in-pond raceway system (IPRS). Fish (initial average body weight: 35.68±2.12g) were randomly allotted to in-pond raceways (26.2m×5m×2.5m) stocked at two stocking densities (68 and 114 fish/m3, respectively). Fish were fed twice daily (08:00 and 17:00), and the daily ration feed was 4% of the body weight. No signi...
Wang Zaigui1,*, Guo Panpan1, Ye Miao1, Sun Linghong1, Zhang Hongfu2 and Liu Chaoliang1,*
...coon quality and amylase activity of silkworm, Bombyx L, we got the following results: (1) In the larval duration of 3rd instar silkworm, average weight gain (AWG) and feed conversion ratio (RFC) were not significantly affected with the use of B. subtilis, but the remaining amount of mulberry leaves (RAML) was significantly decreased contrasting to the blank group (P<0.05). (2) In the larval duration of 4th instar si...
Iqra Jaffar, Zainab Sehzadi, Muhammad Adeel, Khalid P. Lone and Mehwish Faheem*
...r and gills by analyzing activity of glutathione-S-transferase (GST), catalase (CAT), glutathione (GSH) and by measuring lipid peroxidation (LPO). Catalase activity increased significantly in liver and gills of fish exposed to 10μg/l of DBP. Level of GSH and LPO increased significantly in gills while a non-significant decrease was recorded for GSH and GST in liver. These results showed that exposure to low concentrations ...
Rabia Khalid1, Saleema Bashir Shams1, Bibi Nazia Murtaza1,*, Gaitee Joshua1, Saira Mushtaq1, Hassan Al-Talhi2 and Abdulaziz Al-Amri2
...Associations of physical activity and sedentary behaviors with the tendencies of metabolic syndrome were evaluated. The experimental and laboratory values for BMI, total cholesterol (TC), total triglycerides (TG), high density lipoproteins (HDL), low density lipoproteins (LDL) and fasting blood glucose (FBG) were used to demonstrate the incidence of metabolic syndrome. Overall, 39.025% of bank employees and 22.53% of randomly selected control individuals have ...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
... motility, mitochondrial activity, membrane integrity and arosome integrity. The results illustrated that compared with controls, above-mentioned indices of sperm were significantly improved under concentrations of Vitamin C (400 mg/L) and Vitamin E (600 mg/L) (P < 0.05). Moreover, physiological indices of donkey semen were significantly higher than the control groups (P < 0.05) when Vitamin C (200 mg/L) and Vitamin E (200 mg/L) were presen...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
...l cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48 h) was more active, being able to kill 100% of the Acanthamoeba cysts.The results showed the cysticidal activity of T. ammi essential oil, LC50 values estimated to be 73.31, 61.69, ...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...n hepatic catalase (CAT) activity of fish, Channa striata exposed to the sub-lethal concentration (1/3rd of LC50) for 14 days. The CAT was partially purified by using ammonium sulphate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control ...
Muhammad Naveed Sheas1, Haris Rasool2, Muhammad Nouman Rafique2, Muhammad Rizwan Tariq1*, Abid Muhammad1, Khubaib Ali1
...od swings, aversion from activity and apathy which affects the thoughts, behavior and feelings of wellbeing. Honey and curcumin, traditional folk medicines, are thought to steadfast the defense mechanisms of body against oxidative stress which is a key factor in elevating depression. This study is designed to reveal the efficacy of honey and curcumin against depression in rat model. 24 male wister rats were randomly divided into 4 groups. After the trial, bloo...
Xuefeng Cao1, Guangneng Peng1, Xiaobin Gu1, Changliang He1, Guizhou Yue2, Jun Shi3,* and Zhijun Zhong1,*
...and it showed no cross-reactivity with other pathogens. Sensitivity testing showed the minimum detection limits of real-time (RT)-PCR were 7.44×101 copies·μL-1 (CPV) and 4.20×101 copies·μL-1 (CDV). DARPM detection of CPV with DNA showed a minimum detectable of 1.53×101copies μL-1, while the minimum detectable amount from the virus culture supernatant was 6.70×...

Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain

...roteolytic and lipolytic activity with skim milk and Tributyrin agar (TBA plate) assay method. From the collected samples, a total of 20 bacterial isolates (PAK-01 to PAK-20) were exhibiting psychrotrophic physiology. About 8 isolates were capable of proteolysis in alkaline range of pH; and 6 bacteria exhibited lipolysis. Based upon primary and secondary qualitative screening, One isolate (PAK-01) exhibited maximum proteolysis on 1 % skim milk while another is...
Syed Makhdoom Hussain1*, Nisar Ahmad1, Azhar Rasul1, Muhammad Mudassar Shahzad2, Muhammad Latif3, Muhammad Zubair Ul Hassan Arsalan1, Muhammad Umair1  and Hafiza Hina Shafqat1
...g absorbance and surface activity, so the aim of research was to estimate the impact of Cr nanoparticles on growth performance, nutrient digestibility and hematological parameters of C. catla fingerlings fed nano- Cr particles supplemented sunflower meal based diets. The experiment was consisted on seven test diets on the basis of supplementation of nano Cr graded levels (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg Kg-1). Chromic oxide was used as an ine...
Khansa Nazir1,*, Tariq Mukhtar1 and Humayun Javed2
...t study, the nematicidal activity of silver nanoparticles (AgNP) was investigated against the most destructive root-knot nematode (Meloidogyne incognita). The maximum mortality of juveniles was recorded at a concentration of 100 mg/ml followed by 75 mg/ml of AgNP. The minimum mortality was recorded with 25 mg/ml of AgNP. With the increase in concentration, there was a corresponding increase in the mortality of juveniles showing a direct relationship bet...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...ted enzymes, the working activity of separated enzymes was checked by performing desizing test for alpha-amylase and biopolishing test for cellulase. The noteworthy results of desizing and biopolishing test has promoted the significance of unidirectional freezing technique (UFT).
...
Emre Barlas1 and Elif Yamaç2,*
...ion of cave entrance and activity of cave values were determined. Seven out of 10 recorded bat species were evaluated as new records for the area. There was a significant preference for caves which were situated at lower altitude, far from paved road and close to water source. Bats were mostly found in longer caves. Caves with entrance oriented to northwest and southeast were preferred by bats. Undertaking effective conservation measures to maintain especially...

Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3

...aximum level of xylanase activity (9.29 Uml-1), CMCase (7.37 Uml-1), laccase (13.57 Uml-1) and effluent decolorization (69.68 %) were observed on 7th day during shake flask studies.

...
Xiaopeng Tang1,2, Rejun Fang3,4,*, Guochang Pan1,2 and Kangning Xiong1,2,*
...tate dehydrogenase (LDH) activity of isolated small intestine at time on 0 min, 30 min, 60 min, 90 min and 120 min, to provide a appropriate operation time for Ussing chamber test. Then, a total of 20 Loman laying hens with similar weight (1.25±0.13 kg) were divided into 1 of 5 treatments: i) control group (0 ng/mL EGF), ii) 50 ng/mL EGF group, iii) 100 ng/mL EGF group, iv) 150 ng/mL EGF group, and v) 200 ng/mL EGF group for using chamber test. The...
Nouf Alharbi*, Mai Elobeid and Promy Virk
...peroxide dismutase (SOD) activity in liver, malondialdehyde (MDA) levels in liver and serum, and histopathological evaluation of liver and kidney. Results showed that low dose of quercetin was significantly (p≤0.05) more effective than the high dose. Low dose was more efficacious in reducing Cd accumulation in the tissues, reversing the effects of Cd toxicity on blood profile, and on the CAT and SOD activity in the liver ...

Muhammad Arshad1, Muhammad Irfan Ullah1*, Muhammad Afzal1, Mian Anjum Murtaza2, Ejaz Ahraf3, Zahoor Hussain4, Syed Muhammad Ali Zahid1 and Maryam Riaz1 

...ltivars caused by mining activity of CLM during summer 2016. The total leaf area and mine area per leaf were calculated by the image analysis method using Sigma Scan Pro 5.0 software. The results of the present study showed that CLM generated larger mines; 1.64 cm2 on Grapefruit, 1.44 cm2 on Kinnow and 1.40 cm2 on Succari compared to other five cultivars. However, the percent leaf damage due to the feeding of CLM larvae was observed higher 44.2% on Fairchild, ...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...as significant antigenic activity and can potentially be used as recombinant vaccines against HCV infection.
...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
...manganese on antioxidant activity in the fish, Labeo rohita. Fish were acclimatized for two weeks in the cemented tanks prior to the start of experiment. Four groups (n=10) of one year old fingerlings of Labeo rohita were exposed to 96-hr LC50 and sub-lethal concentrations (2/3rd,1/4th and 1/5th of LC50) of manganese for a duration of 30 days in the glass aquaria of 70L water capacity. After ...
Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
...wn to exhibit anticancer activity in various human cancer cells. However, the anticancer mechanism of piceatannol is not well studied. In the meantime, cisplatin (CDDP) is the first line treatment for some tumors. The present study was aimed to explore the cellular targets and anticancer mechanism of piceatannol in human osteosarcoma U2OS cells. Cell proliferation was measured using CCK-8 assay and colony forming assay. Flow cytometry was used to determine apo...
Muhammad Suleman1,*, Abu ul Hassan Faiz2, Muhammad Shahbaz2 and Ayesha Riaz3
...xylanase showed specific activity of 41.22 IU/mg protein. Partially purified xylanase showed protein content of 80.6 mg/ml. The novelty of this study is basic sources used are indigenous and cheap for production of xylanase.
...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...encer. The antibacterial activity of the protein monomer in antlerplate was studied by disc diffusion method, and the antibacterial ring diameter of each antibacterial agent was measured to determine its antibacterial ability. The molecular mass and purity of this polypeptide was 18.970kDa and 90%. Amino acid sequence analyses indicated that the N-terminal amino-acid sequence of this monomeric peptide was AAQSLLACGAARLYKIIQQWPHYRR. The results showed that unde...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...ding capacity and alters activity of GH. Therefore, the GH and GHR genes are considered important candidate genes. DNA samples of Marecha camel were collected from the Camel Breeding and Research Station at Rakhmani Bhakar, Pakistan. Two significant polymorphic sites were identified in GH gene and three in GHR gene. Out of these, T1720A polymorphism in GH and A211927G polymorphism in GHR genes changed the amino acid from leucine to histidine and methionine to ...
Mohammed Al Bratty1,*, Hassan A. Alhazmi1,2, Desam Nagarjuna Reddy3, Abdul Jabbar Al-Rajab3, Sadique A. Javed1 and Zia ur Rehman1,4
...remarkable antimicrobial activity against certain strains of bacteria and yeasts. In general, significant antimicrobial activity was recorded against gram positive bacteria (zone of inhibition: 16.3±0.35–30.6±0.11 mm; MICs: 1–4 μg/ml); while gram negative bacteria (zone of inhibition: 10.1±0.23–17.2±0.13 mm; MICs: 4–16 μg/ml) and yeasts (zone of inhibition: 9.1±...
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
...ve medium. Antibacterial activity of different compounds was observed against the pathogenic bacterial isolates. Minimum Inhibitory Concentration (MIC) test of different kinds of honey was performed against the isolated bacterial pathogens. The antibacterial activity of leaf extract of Azadirachta indica (Neem Plant) was also checked against the pathogenic bacterial isolates. The bacterial pathogens isolated from eye ...

Sameera Arshad1*, Arshad Mahmood Malik2 and Iftikhar Hussain3 

...ing periods of high bird activity.  

...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*

 

...estigated. Dehydrogenase activity was detected by a lactate dehydrogenase assay kit; superoxide dismutase (SOD) activity and malonaldehyde (MDA) concentration were detected by a total superoxide dismutase test kit and a malondialdehyde test kit. Genomic DNA and RNA were extracted from cells; Methylation was inhibited by treating HL7702 cells with 5-aza-2’-deoxycytidine (AZA), and the level was analyzed via the methylat...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
...tains the solubility and activity of the α subunit. In this study, two plasmids were used to produce an active human P4H tetramer (rhP4H) in Pichia pastoris. The P4Hα was cloned into pPICZαA, and PDI was cloned into PHIL. Real-time PCR showed that the P4H gene was expressed at the mRNA level. SDS-PAGE and western blotting analysis showed that the secreted expression of rhP4H was achieved successfully in P. pastoris GS115 cells. ...
Rafiq-ur-Rehman1*, Zahoor Ahmad1, Wiqar Ahmad2, Muhammad Mansoor3 and Shah Masaud1
...nd environmentally sound activity in crops production. Response of mungbean cultivar (Inqalab Mung) inoculated with four strains of rhizobium (TAL-634, TAL-169, NARC-BK and NARC-M) was evaluated during two consecutive years 2015 and 2016 at the Agricultural Research Institute, Rata Kulachi, Dera Ismail Khan (31º 49/ N latitude and 70º 55/ East longitude), Khyber Pakhtunkhwa, Pakistan. The current experiments were carried out in a randomized complete ...

Sarhad Journal of Agriculture

September

Vol. 35, Iss. 3, Pages 663-1019

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