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Utilizing Next-Generation Sequencing to Develop and Characterize Microsatellite Loci in Cuttlefish (Sepia pharaonis) and Cross-Amplification in Other Sepiidaes

Utilizing Next-Generation Sequencing to Develop and Characterize Microsatellite Loci in Cuttlefish (Sepia pharaonis) and Cross-Amplification in Other Sepiidaes

Liqin Liu, Maoting Wang, Zhenming Lü, Li Gong*, ChangWen Wu, Baoying Guo and Lihua Jiang

National Engineering Research Center for Marine Aquaculture, College of Marine Sciences and Technology, Zhejiang Ocean University, Zhoushan 316004, China

*     Corresponding author: gongli1027@163.com

 

ABSTRACT
The cuttlefish Sepia pharaonis, known for its economic value, is distributed in the tropical coastal waters of the Indo-pacific region. In this study, we developed twenty-one microsatellite loci for S. pharaonis through next-generation sequencing technology. A total of 100 alleles were detected, and the number of alleles per loci ranged from 2 to 9. The observed and expected heterozygosities per loci ranged from 0.000 to 0.531 and from 0.031 to 0.751, respectively. Polymorphism information content (PIC) showed that six loci were highly informative (PIC > 0.5). Five loci (CL1142, CL1770, CL2683, CL3494, CL 3674) significantly deviated from the Hardy-Weinberg equilibrium after a Bonferroni correction (P < 0.05), and none of the loci showed linkage disequilibrium. In addition, these loci were cross-amplified in three closelyrelated species. Nineteen, fifteen, and thirteen loci were amplified in Sepia lycidas, Sepia esculenta and Sepiella japonica, respectively. 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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