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Phylogenetic Reconstruction of Ropalida brevita (Vespidae: Polistinae) using 12S rRNA and Cytochrome Oxidase 1 (Co1) Genes

Phylogenetic Reconstruction of Ropalida brevita (Vespidae: Polistinae) using 12S rRNA and Cytochrome Oxidase 1 (Co1) Genes

Muzafar Shah1,*, Mian Sayed Khan1, Muhammad Ather Rafi2, Habib Ahmed3 and James M. Carpenter4

1Department of Zoology, Hazara University, Mansehra-21300, Pakistan
2National Insect Museum, Department of Plant and Environmental Protection, NARC, Islamabad, Pakistan
3Department of Genetics, Hazara University, Mansehra-21300, Pakistan
4Division of Invertebrate Zoology, Richard Gilder Graduate School, American Museum of Natural History, New York NY-10024, USA

*     Corresponding author: muzafarphd@hotmail.com

ABSTRACT
A total of 67 samples of Ropalida brevita were collected from District Abbottabad, Pakistan during 2011 to 2013. Species was confirmed by taxonomic key, which are belonging to subfamily of Polistinae. Phylogenetic tree were reconstructed using 12 SrRNA (12S) gene showing homology of 73% compared with gene bank data while on the basis of cytochrome oxidase 1 (Co1) gene 93% homology were recorded. It was concluded that Co1 gene is more reliable than 12S rRNA gene for confirmation of R. brevita position in the Vespidae family.

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Pakistan Journal of Zoology (Associated Journals)

August

Vol. 49, Iss. 4, Pages 1151-1546

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