Molecular Identification and Sequence Analysis of Dusky Cotton Bug, Oxycarenus hyalinipennis (Hemiptera:Lygaiedae) Infesting Cotton Field in Pakistan
Jam Nazeer Ahmad1,2,*, Muhammad Jafir1, Muhammad Wajid1, Sumaira Maqsood3 and Samina J.N. Ahmad1,2,*
The identification of dusky cotton bug, Oxycarenus hyalinipennis (Hemiptera:Lygaeidae) is difficult because of its close resemblance to other bugs of genus Oxycarenus. Molecular identification and characterization of dusky cotton bug (DCB), Oxycarenus hyalinipennis (Oxycarenidae: Hemiptera) was done through Polymerase chain reaction and sequencing. In PCR, mitochondrial cytochrome oxidase I (COI) gene based primers were used for identification of this pest collected from various cotton fields in Punjab. Phylogenetic analysis was also complemented to differentiate DCB identified from other countries of the world. The PCR bands obtained in gel electrophoresis amplified PCR fragments from all DCB species at 710 bp. The sequencing results and phylogenetic analysis with sequences submitted at NCBI database revealed that DCB have 99-100% similarity with Oxycarenus hyalinipennis OH1 (JQ342987.1), Oxycarenus hyalinipennis OH2 (JQ342988.1) reported from USA. This is the first report of molecular identification and DNA barcoding of Oxycarenus hyalinipennis infesting cotton from Pakistan. DCB is becoming alarming pest, so, this study will be highly helpful to observe DCB correct identification for management.