Isolation and Characterization of 25 Polymorphic Microsatellite Markers of Sepiella japonica
Jiaxin Guo1, Xiumei Zhang1,2, Xiaoyan Wang2, Liqin Liu2 and Tianxiang Gao1,*
Sepiella japonica was once one of the major fisheries in Zhejiang province of China. The availability of highly polymorphic markers was important to conduct the conservation of S. japonica. In this study, next-generation sequencing and de novo assembly were used for potential useful microsatellite markers obtaining of S. japonica. A total of 120 microsatellite markers were designed and tested, 25 primer pairs showed polymorphism among S. japonica individuals. Number of alleles, observed and expected heterozygosity per locus ranged from 2 to 8, 0.083 to 0.922 and 0.042 to 0.866, respectively. The PIC ranged from 0.04 to 0.829. These markers will be useful in evaluation germplasm and genetic composition in the further research.