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Isolation and Characterization of 19 Polymorphic Microsatellite Markers of Sepia esculenta

Isolation and Characterization of 19 Polymorphic Microsatellite Markers of Sepia esculenta

Xiangbin Meng1,Tianxiang Gao2, Chunhou Li3, Na Song1, Lu Liu1, Liqin Liu2 and Xiumei Zhang1,*

1Fishery College, Ocean University of China, Qingdao, China
2National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316004, China
3Key Laboratory for Exploitation & Utilization of Marine Fisheries Resource in South China Sea, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China

ABSTRACT
Sepia esculenta is an economically important marine cephalopoda. The availability of highly polymorphic markers will be important to promote the conservation of this species. We performed next-generation sequencing and de novo assembly to obtain potential useful microsatellite markers for S. esculenta. Among 80 tested microsatellite markers, 19 showed polymorphism among S. esculenta individuals. The allele number of all polymorphic microsatellite markers ranged from 6 to 10. Expected and observed heterozygosity varied from 0.260 to 0.904 and from 0.292 to 0.861, with an average of 0.741 and 0.607, respectively. The polymorphism information content (PIC) ranged from 0.566 to 0.823. These markers will be useful in further studies and will give new insight into conservation and efficient management of this species.

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Pakistan Journal of Zoology

August

Vol. 50, Iss. 4, Pages 1199-1600

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