Isolation and Characterization of Microsatellite Markers for the Sable, Martes zibellina (Mammalia: Mustelidae)
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,*
The number of wild sables has declined dramatically in recent years due to the high value and increasing demand of their fur. In order to evaluate the genetic diversity and population structure for the conservation and management of sables (Martes zibellina), we developed six microsatellite markers using a method of fast isolation by AFLP of sequences containing repeats (FIASCO) from them in this study. The primers designed by Primer Premier 5.0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged from two to five and the mean number of alleles was 3.67. The observed and expected heterozygosities ranged from 0.227 to 0.545 and 0.201 to 0.630, respectively. The polymorphic information content value ranged from 0.181 to 0.573. Null allele frequency varied from -0.0548 to 0.0563. The sets of loci proved to correctly discriminate populations using Principal Coordinate analysis. These polymorphic markers will be useful tools for investigating genetic diversity and population structure of this species.