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Utilization of Emulsiflex Prepared Escherichia coli Strain EC4 Oxyrase for Improved Cultivation of Anaerobic Bacteria by using Hungate Technique and Determination of its Kinetic Parameters

Utilization of Emulsiflex Prepared Escherichia coli Strain EC4 Oxyrase for Improved Cultivation of Anaerobic Bacteria by using Hungate Technique and Determination of its Kinetic Parameters

Muhammad Usman Ahmadand Ikram-ul-Haq*

Institute of Industrial Biotechnology, GC University, Lahore-54000

*      Corresponding author: ikmhaq@yahoo.com

 

Fig. 1.

Phylogenetic tree showing evolutionary relationship between isolated E. coli strain EC4 (oxyrase strain) and some reference strains. The number at the branch nodes indicates bootstrap values (%) based on 1000 replication. The scale bar represents 0.0005 nucleotide substitutes per position.

Fig. 2.

Effect of time on oxyrase activity (emulsiflex mediated prepared membrane fragments); 57°C temperature, pH 8.5 and 25mM lactate as H+ donor. Y-error bars represents the standard deviation among the triplicates. Each value is an average of three replicates, data labels followed by different letters differs significantly at ∝=0.05.

Fig. 3.

Effect of initial concentration of H+ donors on oxyrase activity; time 1.5 min, 57°C temperature, pH 8.5. Y-error bars represents the standard deviation among the triplicates. Each value is an average of three replicates, data labels followed by different letters differs significantly at ∝=0.05.

Fig. 4.

Comparison and effect of Cys-HCl and E. coli oxyrase on growth pattern of Anaerobaculum hydrogeniformans OS1 (A), Akkermansia muciniphila (B), Bilophila wadsworthia (C) and Roseburia intestinalis (D). Y-error bars represents the standard deviation among the triplicates. Each value is an average of three replicates, data labels followed by different letters differs significantly at ∝=0.05.

Fig. 5.

Double reciprocal Lineweaver-Burk plot between inverse of V and inverse of different concentrations of various substrates for emulsiflex prepared oxyrase membrane fragments at 57°C, pH 8.5 and time 1.5 min.

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Pakistan Journal of Zoology

August

Vol. 51, Iss. 4, Pages 1203-1598

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