In this study insecticides toxic effects of mixture of organochlorine (endosulfan) and organophosphate (chlorpyrifos) investigated on the activity of antioxidant enzymes viz. superoxide dismutase, peroxidase, catalase and glutathione S-transferase in different organs (liver, gills, kidney, brain, heart and muscle) of fish, Labeo rohita. The LC50 of chlorpyrifos+endosulfan mixture was calculated as 1.95±0.02 μgL-1 for 96 h with the 95% confidence limits. The fish expose to the mixture (1:1) for 96-h. The results obtained from this study showed that superoxide dismutase, peroxidase and glutathione S-transferase activities in the liver, gills, kidney, brain, heart and muscle were significantly (P<0.05) increased compared to control. The superoxide dismutase activity in organs of fish followed the order: liver>brain>kidney>gills>heart>muscle. The mean peroxidase activity in L. rohita followed the pattern: liver>brain>gills>kidney>heart>muscle. The glutathione S-transferse activity followed the order: brain>muscle>liver>gills>kidney>heart. However, catalase activity was significantly (P<0.05) increased in liver, gills and kidney of pesticides mixture exposed L. rohita as compared to control while it was decreased in brain, heart and muscle.