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The Expression of Cytoplasmic and Membrane Proteins in Dog Adipose-Derived Stem Cells on Different Passages During Cultivation in Vitro

The Expression of Cytoplasmic and Membrane Proteins in Dog Adipose-Derived Stem Cells on Different Passages During Cultivation in Vitro

Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*

1Department of Physiology, Pathophysiology and Immunology of Animals, National University of Life and Environmental Sciences of Ukraine, 03041 Kyiv, Ukraine
2Department of Experimental Cell Systems, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, 03022 Kyiv, Ukraine
3Hospital of Veterinary Medicine, 03022 Kyiv, Ukraine 
4Institute of Biological Basis of Animal Production, University of Life Sciences in Lublin, Poland

*      Corresponding author: magdalena.gryzinska@up.lublin.pl

Fig. 1.

Native unstained culture DADSCs: a) IVth passage, 100×; b) Хth passage, × 200.

Fig. 2.

Immunophenotypic characterisation of dog adipose derived mesenchymal stem cells: a) control; b) E-cadherin-positive cells; c) actin-positive cells; d) N-cadherin-negative cells; e) CD44; f) Pan cytokeratin; g) Vimentin-positive cells x 400.

Pakistan Journal of Zoology

August

Vol. 52, Iss. 4, Pages 1225-1630

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