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The Effect of Hemodialysis on the Expression of CXCL8 and its mRNA in Neutrophils of the Patients with Chronic Renal Failure

The Effect of Hemodialysis on the Expression of CXCL8 and its mRNA in Neutrophils of the Patients with Chronic Renal Failure

Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1

1Department of Aetiology and Immunology, Medical College, Anhui University of Science and Technology, Huainan 232001, China
2Department of Medicine, the First Affiliated Hospital of Anhui University of Science and Technology (the First People’s Hospital of Huainan), Huainan 232007, China

Jian Wang and Zhi Liu have contributed equally to this work and should be considered as co-first authors.

*      Corresponding author: wangjian8237@sina.com

 

Fig. 1.

The electrophorogram of total RNA from PBNs.

Fig. 2.

Expression of CXCL8 mRNA in the PBNs of chronic renal failure. M, DL2000; 1 and 2, GAPDHC and CXCL8 of normal control; 3, 4, 5 and 6, GAPDH and CXCL8 of CRF without infection (patient 1 and patient 2); 7, 8, 9 and 10, GAPDH and CXCL8 of CRF with infection (patient 3 and patient 4).

Fig. 3.

levels of CXCL8 in serum (A) and CXCL8 mRNA in PBMCs (B) of chronic renal failure.

Fig. 4.

The counts of neutrophil in peripheral blood of the patients with chronic renal failure.

Fig. 5.

Relationship of CXCL8 in serum and its mRNA in PBNs in patients of chronic renal failure (before hemodialysis).

Fig. 6.

Relationship of CXCL8 in serum and its mRNA in PBNs in patients of chronic renal failure (after hemodialysis for 12h).

Fig. 7.

Relationship of CXCL8 in serum and its mRNA in PBNs in patients of chronic renal failure (after hemodialysis for 3d).

Fig. 8.

Neutrophil and its major receptors. Neutrophil can express CXCR1, CXCR2, FcR and other a variety of chemokine receptors, which may combine with the corresponding ligands, and further participate in local and systemic inflammatory damage and repair. IP-10, interferon-inducible protein-10; Mig, monokine induced by the interferon-gamma.

Fig. 9.

The main process of hemodialysis and its activation on neutrophils and mononuclear macrophages. When multiple toxic metabolites in the blood stream were filtered by hemodialyzer, the activity of neutrophils and mononuclear macrophages in the circulation gradually increased.

Pakistan Journal of Zoology

April

Vol. 51, Iss. 2, Pages 399-799

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