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Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review

Inam Ul Haq1*, Humara Umar2, Naeem Akhtar3, Muhammad Azhar Iqbal2 and Muhammad Ijaz1

1Barani Agricultural Research Institute (BARI), Chakwal 48800, Pakistan; 2Centre of Excellence for Olive Research and Training at BARI Chakwal, Chakwal 48800, Pakistan; 3Pakistan Oilseed Development Board (PODB), Islamabad 44000, Pakistan.

*Correspondence | Inam Ul Haq, Barani Agricultural Research Institute (BARI), Chakwal 48800, Pakistan; Email:


Micropropagation is one of the most significant applications of biotechnology in the field of horticulture. This technique is used for proliferation of olive (Olea europaea L.) tree which is globally cultivated for centuries and its value added products are consumed worldwide. The traditional propagation techniques of olive are uneconomical with wastage of time while micropropagation of olive may expedite the development of important characteristics like biotic and abiotic resistance with high yield and premium quality olive oil. However, the commercial micropropagation of olive trees is currently not enough because of production cost and globally; it is mostly used for research and development on various aspects of olives. In this review, efforts are made to document the different techniques for successful initiation of olive micropropagation techniques because presently in Pakistan this technique is used at very limited research and development scale besides having basic infrastructure and necessary facilities. Therefore, authors planned to document different critical stages of olive micropropagation like; media preparation, culture establishment, shoot and root proliferation and acclimatization which would be applied for micropropagation of olives at Barani Agricultural Research Institute (BARI), Chakwal. Resultantly, olive expansion through micropropagation can be predicted in near future for sustainability of olive sector in Pakistan.


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Pakistan Journal of Agricultural Research


Vol. 35, Iss. 1, Pages 1-258


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