Submit or Track your Manuscript LOG-IN

Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Synthetic Oviductal Fluid Medium with Isologous Oviductal Epithelial Cells Improves the Development of In Vitro Produced Nili Ravi Buffalo (Bubalus bubalis) Embryos

Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*

1Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar 25000
2Animal Physiology Laboratory, Department of Zoology, PMAS-Arid Agriculture University, Rawalpindi 46300
3Department of Zoology, University of Education, Jauharabad Campus, Jauharabad-41200
4Buffalo Research Institute Pattoki, Kasur, Lahore 54000
5Department of Animal Reproduction, Riphah International University, Lahore 54000

*      Corresponding author: shamim@uaar.edu.pk.com

 

ABSTRACT

The objective of the current study was to investigate and compare the role of buffalo oviductal epithelial cell (BOEC) co-culture and conditioned culture media, on in vitro development of Nili Ravi buffalo embryos. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium alone, Group II: SOF + co-culture, Group III: Conditioned SOF, Group IV: M199 alone, Group V: M199 + co-culture and Group VI: Conditioned M199 for in vitro culture (IVC). The percentage of embryos capable of crossing 8-16 cell block and reaching morula stage were higher (P < 0.05) in Group II and Group III compared to other treatments. Both treatment groups were equally effective (P > 0.05). Results also indicated that synthetic oviductal fluid (SOF) medium was found better than M199 when co-cultured and conditioned with buffalo oviductal epithelial cells for culturing buffalo embryos. Conditioned SOF is recommended to be used as IVC media in future studies as it eliminates contamination problem, remove batch to batch variation, long-lived and is readily available.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe